Fatty acid production and associated gene pathways are altered by increased salinity and dimethyl sulfoxide treatments during cryopreservation of Symbiodinium pilosum (Symbiodiniaceae)
In this study, we investigated the role of increased salinity on FA production and the genes involved in FA biosynthesis and degradation pathways during the cryopreservation of Symbiodinium pilosum. Overall, there was a twofold increase in mass of FAs produced by S. pilosum after being cultured in medium with increased salinity (54 parts per thousand; ppt). Dimethyl sulfoxide (Me2SO) led to a ninefold increase of FAs in standard salinity (SS) treatment, compared to a fivefold increase in increased salinity (IS) treatments. The mass of the FA classes returned to baseline during recovery. Transcriptomic analyses showed an ac...
Source: Cryobiology - February 1, 2024 Category: Biology Authors: Joseph K Kihika John K Pearman Susanna A Wood Lesley L Rhodes Kirsty F Smith Matthew R Miller Juliette Butler Ken G Ryan Source Type: research
Enhancing cryopreservation survival in Petunia × Calibrachoa 'Light Yellow' callus: Insights into material characteristics and genetic integrity
Cryobiology. 2024 Jan 29;114:104846. doi: 10.1016/j.cryobiol.2024.104846. Online ahead of print.ABSTRACTPetunia × Calibrachoa 'Light Yellow' (× Petchoa 'Light Yellow') is a kind of perennial herbaceous flower obtained through intergeneric hybridization of Petunia and Calibrachoa with high ornamental value and wide application, facing challenges in seed acquisition. Expanding propagation through tissue culture is an economically efficient means. Hence, establishing an effective procedure for the storage of callus is essential for × Petchoa 'Light Yellow'. Cryopreservation is an effective method for the in vitro propagati...
Source: Cryobiology - January 31, 2024 Category: Biology Authors: Yuanshan Zhang Xiangxin Guo Junyan Song Guanqun Chen Xiaohui Shen Source Type: research
Preserving frozen stallion sperm on dry ice using polymers that modulate ice crystalization kinetics
In this study, we determined critical temperatures for storage of cryopreserved stallion sperm. We evaluated: (i) effects of cooling samples to different subzero temperatures (-10 °C to -80 °C) prior to storing in LN2, (ii) stability at different storage temperatures (i.e., in LN2, dry ice, -80 °C and -20 °C freezers, 5 °C refrigerator), and (iii) sperm cryosurvival during storage on dry ice (i.e., when kept below -70 °C and during warming). Furthermore, (iv) we analyzed if addition of synthetic polymers (PVP-40, Ficoll-70) modulates ice crystallization kinetics and improves stability of cryopreserved specimens. Sper...
Source: Cryobiology - January 31, 2024 Category: Biology Authors: Laura Uhlmannsiek Hang Shen Heinke Eylers Gunilla Martinsson Harald Sieme Willem F Wolkers Harri ëtte Oldenhof Source Type: research
Cysteine supplementation pre-freeze and post-thaw improves integrity and reduces oxidative stress in cryopreserved ram spermatozoa
This study aimed to determine the effect of concentration and timing of cysteine supplementation on the integrity and function of cryopreserved ram spermatozoa. Nine ejaculates were collected from three Texel rams then cryopreserved and supplemented with cysteine (0, 0.5, or 1.0 mg/mL) added pre-freeze (PF), post-thaw (PT) or pre-freeze and post-thaw (PF + PT) generating seven treatments: 1) control 0 mg/mL, 2) PF 0.5 mg/mL, 3) PF 1 mg/mL, 4) PT 0.5 mg/mL, 5), PT 1.0 mg/mL, 6) PF + PT 0.5 mg/mL and 7) PF + PT 1.0 mg/mL. Sperm motility, viability, acrosome integrity, ROS production and penetrability through artificial cervi...
Source: Cryobiology - January 29, 2024 Category: Biology Authors: Ahmed Kafi Muhammad Khalid Tharangani Herath Claire Kershaw Source Type: research
Cryopreservation with DMSO affects the DNA integrity, apoptosis, cell cycle and function of human bone mesenchymal stem cells
Cryobiology. 2024 Jan 19;114:104847. doi: 10.1016/j.cryobiol.2024.104847. Online ahead of print.ABSTRACTCryopreservation (CP) enables pooling and long-term banking of various types of cells, which is indispensable for the cell therapeutics. Dimethyl sulfoxide (DMSO) is universally used as a cryoprotectant in basic and clinical research. Although, the use of DMSO has been under serious debate due to significant clinical side effects correlated with infusions of cellular therapy products containing DMSO, the effect of CP with DMSO on the cell properties and functions remains unknown. Here, we experimentally found that the CP...
Source: Cryobiology - January 21, 2024 Category: Biology Authors: Yanqin Ding Shuo Liu Jianting Liu Shenglin Jin Jianjun Wang Source Type: research
Irreversible lipid phase transition detected in a porcine oocyte at chilling
Cryobiology. 2024 Jan 17;114:104850. doi: 10.1016/j.cryobiol.2024.104850. Online ahead of print.ABSTRACTUnder physiological conditions, the membranes and lipid droplets of germ cells are in a conformationally disordered phase. Typically, during cooling, lipids undergo the transition to ordered phases and, upon heating, melt into a disordered phase. In this communication, we report the lipid phase transition in lipid droplets observed in porcine oocytes. Upon cooling, a sharp lipid phase transition from conformationally disordered to ordered state was detected within the temperature range between 20 and 15 °C. Subsequent h...
Source: Cryobiology - January 19, 2024 Category: Biology Authors: K A Okotrub A N Omelchenko E A Chuyko S Y Amstislavsky N V Surovtsev Source Type: research
Effects of alpha lipoic acid supplementation on post-thaw quality of drone semen
This study aimed to determine the effect of alpha-lipoic acid (ALA) on post-thaw quality of bee semen. In the study, semen from sexually mature drone were collected. A series of experiments were carried out in which the retrieved semen was diluted with diluents containing different ALA concentrations or without ALA supplement (control). Cryopreserved sperm were thawed, and evaluated for motility (phase-contrast microscope), plasma and acrosomal membrane integrity, mitochondrial membrane potential, and DNA fregmantation. The results obtained showed that the highest motility after thawing was observed in the groups containin...
Source: Cryobiology - January 19, 2024 Category: Biology Authors: Ahmet Aktar Selvinar Cakmak Mehmed Berk Toker İbrahim Cakmak Kemal Ba ğci Ümit Can Uzun Selim Alcay Source Type: research
Effect of type and concentration of antioxidant on sperm motility, viability, and DNA integrity of climbing perch Anabas testudineus Bloch, 1792 (Pisces: Anabantidae) post-cryopreservation
Cryobiology. 2024 Jan 17;114:104851. doi: 10.1016/j.cryobiol.2024.104851. Online ahead of print.ABSTRACTSperm quality is preserved through the crucial involvement of antioxidants, which play a vital role in minimizing the occurrence of reactive oxygen species (ROS) during the cryopreservation process. The suitability of the type and concentration of antioxidants are species-dependent, and this study is crucial in order to improve the quality of the climbing perch sperm post-cryopreservation. Therefore, this study aimed to determine the best type and concentration of antioxidants for cryopreservation of climbing perch Anaba...
Source: Cryobiology - January 18, 2024 Category: Biology Authors: Siti Maulida Kartini Eriani Nur Fadli Mohd N Siti-Azizah Filiz K Kocabas Mehmet Kocabas Martin Wilkes Luvi S Handayani Sri R Rahayu Zainal A Muchlisin Source Type: research
Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm
This study documents the effect of cryopreservation on motility, DNA integrity, and gene expression in Mugil cephalus sperm. Fresh sperm were cryopreserved using V2 extender (V2E) or 0.3 M glucose, each in combination with one of three cryoprotective agents (CPAs), i.e., 10 % of dimethylsulfoxide, ethylene glycol, or glycerol, all at once. After two different storage (7- vs 60- day) periods in liquid nitrogen, sperm samples were thawed. Single-cell gel electrophoresis was used to detect the DNA integrity. Heat shock proteins (HSPs), HSP70, HSP90 and glutathione peroxidase (GPx2) genes mRNA expression levels was documented ...
Source: Cryobiology - January 14, 2024 Category: Biology Authors: Ramachandran Balamurugan Sundaram Karthik Venkatesan Arul Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4;114:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellula...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical CA with the Visual-ICE (Boston Scientific) system, a clinically approved and widely utilized device. We found that increasing the duration of freezing from 1 to 2 min increased ice radius from 3.44 ± 0.13 mm to 5.29 ± 0.16 mm, and decreased the minimum temperature achieved from -22.8 ± 1.3 °C to -45.5 ± 7.9 °C. Furthermore, an additional minute of freezing increased the amount of cell death within a 5 mm radius from 42.5 ± 8.9% to 84.8 ± 1.1%. Freezing at 100% intensity leads to faste...
Source: Cryobiology - January 3, 2024 Category: Biology Authors: Maura R Vrabel Christopher C Fesmire Matthew J Rich Robert L Kobrin Michael B Sano David A Zaharoff Source Type: research
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical CA with the Visual-ICE (Boston Scientific) system, a clinically approved and widely utilized device. We found that increasing the duration of freezing from 1 to 2 min increased ice radius from 3.44 ± 0.13 mm to 5.29 ± 0.16 mm, and decreased the minimum temperature achieved from -22.8 ± 1.3 °C to -45.5 ± 7.9 °C. Furthermore, an additional minute of freezing increased the amount of cell death within a 5 mm radius from 42.5 ± 8.9% to 84.8 ± 1.1%. Freezing at 100% intensity leads to faste...
Source: Cryobiology - January 3, 2024 Category: Biology Authors: Maura R Vrabel Christopher C Fesmire Matthew J Rich Robert L Kobrin Michael B Sano David A Zaharoff Source Type: research
