Effect of cryopreservation on motility, DNA integrity and gene expression in grey mullet, Mugil cephalus sperm
This study documents the effect of cryopreservation on motility, DNA integrity, and gene expression in Mugil cephalus sperm. Fresh sperm were cryopreserved using V2 extender (V2E) or 0.3 M glucose, each in combination with one of three cryoprotective agents (CPAs), i.e., 10 % of dimethylsulfoxide, ethylene glycol, or glycerol, all at once. After two different storage (7- vs 60- day) periods in liquid nitrogen, sperm samples were thawed. Single-cell gel electrophoresis was used to detect the DNA integrity. Heat shock proteins (HSPs), HSP70, HSP90 and glutathione peroxidase (GPx2) genes mRNA expression levels was documented ...
Source: Cryobiology - January 14, 2024 Category: Biology Authors: Ramachandran Balamurugan Sundaram Karthik Venkatesan Arul Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4;114:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellula...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
Sperm plasma membrane ion transporters and male fertility potential: A perspective under the prism of cryopreservation
Cryobiology. 2024 Jan 4:104845. doi: 10.1016/j.cryobiol.2023.104845. Online ahead of print.ABSTRACTIntracellular calcium homeostasis plays a crucial role in spermatozoa by regulating physiological functions associated with sperm quality and male fertility potential. Intracellular calcium fine balance in the sperm cytoplasm is strictly dependent on sperm surface channels including the CatSper channel. CatSpers' role is to ensure the influx of extracellular calcium, while intracellular pH alkalinization serves as a stimulus for the activation of several channels, including CatSper. Overall, the generation of intracellular ca...
Source: Cryobiology - January 6, 2024 Category: Biology Authors: Sevastiani Antonouli Valentina Di Nisio Christina Messini Maria Samara Andres Salumets Alexandros Daponte George Anifandis Source Type: research
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical CA with the Visual-ICE (Boston Scientific) system, a clinically approved and widely utilized device. We found that increasing the duration of freezing from 1 to 2 min increased ice radius from 3.44 ± 0.13 mm to 5.29 ± 0.16 mm, and decreased the minimum temperature achieved from -22.8 ± 1.3 °C to -45.5 ± 7.9 °C. Furthermore, an additional minute of freezing increased the amount of cell death within a 5 mm radius from 42.5 ± 8.9% to 84.8 ± 1.1%. Freezing at 100% intensity leads to faste...
Source: Cryobiology - January 3, 2024 Category: Biology Authors: Maura R Vrabel Christopher C Fesmire Matthew J Rich Robert L Kobrin Michael B Sano David A Zaharoff Source Type: research
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical CA with the Visual-ICE (Boston Scientific) system, a clinically approved and widely utilized device. We found that increasing the duration of freezing from 1 to 2 min increased ice radius from 3.44 ± 0.13 mm to 5.29 ± 0.16 mm, and decreased the minimum temperature achieved from -22.8 ± 1.3 °C to -45.5 ± 7.9 °C. Furthermore, an additional minute of freezing increased the amount of cell death within a 5 mm radius from 42.5 ± 8.9% to 84.8 ± 1.1%. Freezing at 100% intensity leads to faste...
Source: Cryobiology - January 3, 2024 Category: Biology Authors: Maura R Vrabel Christopher C Fesmire Matthew J Rich Robert L Kobrin Michael B Sano David A Zaharoff Source Type: research
A novel in vitro model of clinical cryoablation to investigate the transition zone for focal tumor ablation
In this study, we explored the relationship between temperature, ice growth, and cell death using a novel in vitro model of clinical CA with the Visual-ICE (Boston Scientific) system, a clinically approved and widely utilized device. We found that increasing the duration of freezing from 1 to 2 min increased ice radius from 3.44 ± 0.13 mm to 5.29 ± 0.16 mm, and decreased the minimum temperature achieved from -22.8 ± 1.3 °C to -45.5 ± 7.9 °C. Furthermore, an additional minute of freezing increased the amount of cell death within a 5 mm radius from 42.5 ± 8.9% to 84.8 ± 1.1%. Freezing at 100% intensity leads to faste...
Source: Cryobiology - January 3, 2024 Category: Biology Authors: Maura R Vrabel Christopher C Fesmire Matthew J Rich Robert L Kobrin Michael B Sano David A Zaharoff Source Type: research
EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application
Cryobiology. 2023 Dec 27;114:104843. doi: 10.1016/j.cryobiol.2023.104843. Online ahead of print.ABSTRACTCoolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To overcome this challenge, we present our pilot study by introducing the EtOH-LN workflow, which demonstrates the feasibility of using 95 % ethanol as a safer and easily accessible alternative to existing coolants for LN-based c...
Source: Cryobiology - December 29, 2023 Category: Biology Authors: Yong Chiang Tan Muaatamarulain Mustangin Nurwahyuna Rosli Wan Syahira Ellani Wan Ahmad Kammal Nurismah Md Isa Teck Yew Low Pey Yee Lee Dinesh Kumar Chellappan Razman Jarmin Zamri Zuhdi Azlanudin Azman Chik Ian Nursuhadah Mohamed Yusof Lay Cheng Lim Source Type: research
CPA toxicity screening of cryoprotective solutions in rat hearts
Cryobiology. 2023 Dec 27:104842. doi: 10.1016/j.cryobiol.2023.104842. Online ahead of print.ABSTRACTIn clinical practice, donor hearts are transported on ice prior to transplant and discarded if cold ischemia time exceeds ∼5 h. Methods to extend these preservation times are critically needed, and ideally, this storage time would extend indefinitely, enabling improved donor-to-patient matching, organ utilization, and immune tolerance induction protocols. Previously, we demonstrated successful vitrification and rewarming of whole rat hearts without ice formation by perfusion-loading a cryoprotective agent (CPA) solution pr...
Source: Cryobiology - December 29, 2023 Category: Biology Authors: Casey J Kraft Bat-Erdene Namsrai Diane Tobolt Michael L Etheridge Erik B Finger John C Bischof Source Type: research
EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application
Cryobiology. 2023 Dec 27;114:104843. doi: 10.1016/j.cryobiol.2023.104843. Online ahead of print.ABSTRACTCoolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To overcome this challenge, we present our pilot study by introducing the EtOH-LN workflow, which demonstrates the feasibility of using 95 % ethanol as a safer and easily accessible alternative to existing coolants for LN-based c...
Source: Cryobiology - December 29, 2023 Category: Biology Authors: Yong Chiang Tan Muaatamarulain Mustangin Nurwahyuna Rosli Wan Syahira Ellani Wan Ahmad Kammal Nurismah Md Isa Teck Yew Low Pey Yee Lee Dinesh Kumar Chellappan Razman Jarmin Zamri Zuhdi Azlanudin Azman Chik Ian Nursuhadah Mohamed Yusof Lay Cheng Lim Source Type: research
CPA toxicity screening of cryoprotective solutions in rat hearts
Cryobiology. 2023 Dec 27:104842. doi: 10.1016/j.cryobiol.2023.104842. Online ahead of print.ABSTRACTIn clinical practice, donor hearts are transported on ice prior to transplant and discarded if cold ischemia time exceeds ∼5 h. Methods to extend these preservation times are critically needed, and ideally, this storage time would extend indefinitely, enabling improved donor-to-patient matching, organ utilization, and immune tolerance induction protocols. Previously, we demonstrated successful vitrification and rewarming of whole rat hearts without ice formation by perfusion-loading a cryoprotective agent (CPA) solution pr...
Source: Cryobiology - December 29, 2023 Category: Biology Authors: Casey J Kraft Bat-Erdene Namsrai Diane Tobolt Michael L Etheridge Erik B Finger John C Bischof Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
