A review of recent developments in the application of nanostructures for sperm cryopreservation
Cryobiology. 2024 Mar 28:104890. doi: 10.1016/j.cryobiol.2024.104890. Online ahead of print.ABSTRACTIn the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased osmotic pressure, ice crystal formation, and thermal shock, therefore decreasing the quality of sperm. The nanostructures due to their inherent features such as reactivity, high uptake, active surface area, and antioxidant activity, have contributed to modifying freezing prot...
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Saeed Zandiyeh Hamid Kalantari Ali Fakhri Maryam Nikkhah Baadal Jushi Janani Marjan Sabbaghian Source Type: research
On the universality of viscosity in supersaturated binary aqueous sugar solutions: Cryopreservation by vitrification
We present the construction of the Supplemented Phase Diagram and the non-equilibrium nature of the undersaturated-supersaturated kinetic transition. The description of its thermodynamic nature is achieved through the study of behavior of their viscosity as temperature is lowered and concentration increased. In this work, we find a universal character for the viscosities of several sugar water solutions.PMID:38555011 | DOI:10.1016/j.cryobiol.2024.104886 (Source: Cryobiology)
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Soledad Ruiz-Matus Patricia Goldstein Source Type: research
A review of recent developments in the application of nanostructures for sperm cryopreservation
Cryobiology. 2024 Mar 28:104890. doi: 10.1016/j.cryobiol.2024.104890. Online ahead of print.ABSTRACTIn the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased osmotic pressure, ice crystal formation, and thermal shock, therefore decreasing the quality of sperm. The nanostructures due to their inherent features such as reactivity, high uptake, active surface area, and antioxidant activity, have contributed to modifying freezing prot...
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Saeed Zandiyeh Hamid Kalantari Ali Fakhri Maryam Nikkhah Baadal Jushi Janani Marjan Sabbaghian Source Type: research
On the universality of viscosity in supersaturated binary aqueous sugar solutions: Cryopreservation by vitrification
We present the construction of the Supplemented Phase Diagram and the non-equilibrium nature of the undersaturated-supersaturated kinetic transition. The description of its thermodynamic nature is achieved through the study of behavior of their viscosity as temperature is lowered and concentration increased. In this work, we find a universal character for the viscosities of several sugar water solutions.PMID:38555011 | DOI:10.1016/j.cryobiol.2024.104886 (Source: Cryobiology)
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Soledad Ruiz-Matus Patricia Goldstein Source Type: research
A review of recent developments in the application of nanostructures for sperm cryopreservation
Cryobiology. 2024 Mar 28:104890. doi: 10.1016/j.cryobiol.2024.104890. Online ahead of print.ABSTRACTIn the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased osmotic pressure, ice crystal formation, and thermal shock, therefore decreasing the quality of sperm. The nanostructures due to their inherent features such as reactivity, high uptake, active surface area, and antioxidant activity, have contributed to modifying freezing prot...
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Saeed Zandiyeh Hamid Kalantari Ali Fakhri Maryam Nikkhah Baadal Jushi Janani Marjan Sabbaghian Source Type: research
On the universality of viscosity in supersaturated binary aqueous sugar solutions: Cryopreservation by vitrification
We present the construction of the Supplemented Phase Diagram and the non-equilibrium nature of the undersaturated-supersaturated kinetic transition. The description of its thermodynamic nature is achieved through the study of behavior of their viscosity as temperature is lowered and concentration increased. In this work, we find a universal character for the viscosities of several sugar water solutions.PMID:38555011 | DOI:10.1016/j.cryobiol.2024.104886 (Source: Cryobiology)
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Soledad Ruiz-Matus Patricia Goldstein Source Type: research
A review of recent developments in the application of nanostructures for sperm cryopreservation
Cryobiology. 2024 Mar 28:104890. doi: 10.1016/j.cryobiol.2024.104890. Online ahead of print.ABSTRACTIn the 1970s, sperm cryopreservation was presented as a unique route to fertility preservation. The ability to cryopreserve sperm from all species is challenging. The sperm cryopreservation process encompasses various cellular stresses such as increased osmotic pressure, ice crystal formation, and thermal shock, therefore decreasing the quality of sperm. The nanostructures due to their inherent features such as reactivity, high uptake, active surface area, and antioxidant activity, have contributed to modifying freezing prot...
Source: Cryobiology - March 30, 2024 Category: Biology Authors: Saeed Zandiyeh Hamid Kalantari Ali Fakhri Maryam Nikkhah Baadal Jushi Janani Marjan Sabbaghian Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells
This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Yusuke Hayashi Yuki Uno Masahiro Kino-Oka Hirokazu Sugiyama Source Type: research
Cryopreserved leukapheresis material can Be transferred from controlled rate freezers to ultracold storage at warmer temperatures without affecting downstream CAR-T cell culture performance and in-vitro functionality
This study aims to determine the optimal temperature range for transferring cryopreserved leukapheresis materials from two distinct types of controlled rate freezing systems, Liquid Nitrogen (LN2)-based and LN2-free Conduction Cooling-based, to the ultracold LN2 storage freezer (≤-135 °C), and its impact on CAR T-cell production and functionality. Presented findings demonstrate that there is no significant influence on CAR T-cell expansion, differentiation, or downstream in-vitro function when employing a transfer temperature range spanning from -30 °C to -80 °C for the LN2-based controlled rate freezers as well as fo...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Jiaming Wei Katherine Chaney Woo Jin Shim Heyu Chen Grace Leonard Sean O'Brien Ziyan Liu Jinlin Jiang Robert Ulrey Source Type: research
Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells
This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104...
Source: Cryobiology - March 21, 2024 Category: Biology Authors: Yusuke Hayashi Yuki Uno Masahiro Kino-Oka Hirokazu Sugiyama Source Type: research
