CPA toxicity screening of cryoprotective solutions in rat hearts
Cryobiology. 2023 Dec 27:104842. doi: 10.1016/j.cryobiol.2023.104842. Online ahead of print.ABSTRACTIn clinical practice, donor hearts are transported on ice prior to transplant and discarded if cold ischemia time exceeds ∼5 h. Methods to extend these preservation times are critically needed, and ideally, this storage time would extend indefinitely, enabling improved donor-to-patient matching, organ utilization, and immune tolerance induction protocols. Previously, we demonstrated successful vitrification and rewarming of whole rat hearts without ice formation by perfusion-loading a cryoprotective agent (CPA) solution pr...
Source: Cryobiology - December 29, 2023 Category: Biology Authors: Casey J Kraft Bat-Erdene Namsrai Diane Tobolt Michael L Etheridge Erik B Finger John C Bischof Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
Evaluation of the effects of hydroxytyrosol on human sperm parameters during cryopreservation
Cryobiology. 2023 Dec 16;114:104840. doi: 10.1016/j.cryobiol.2023.104840. Online ahead of print.ABSTRACTHuman sperm cryopreservation is a routine procedure in assisted reproductive technology, but it has detrimental effects on different sperm parameters due to oxidative stress. Our objective was to assess the impacts of hydroxytyrosol (HT), as an antioxidant, on human sperm parameters following cryopreservation. In the first phase, 20 normal human semen samples were cryopreserved using the rapid freezing method with different concentrations of HT including 0, 50, 100, 150, and 200 μg/mL. In the second phase, 20 normal sem...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Mojtaba Baharsaadi Maryam Hezavehei Abdolhossein Shahverdi Iman Halvaei Source Type: research
Determining the optimal time interval between sample acquisition and cryopreservation when processing immature testicular tissue to preserve fertility
Cryobiology. 2023 Dec 16;114:104841. doi: 10.1016/j.cryobiol.2023.104841. Online ahead of print.ABSTRACTThe cryopreservation of immature testicular tissue (ITT) prior to gonadotoxic therapy is crucial for fertility preservation in prepubertal boys with cancer. However, the optimal holding time between tissue collection and cryopreservation has yet to be elucidated. Using the bovine model, we investigated four holding times (1, 6, 24, and 48 h) for ITTs before cryopreservation. Biopsies from two-week-old calves were stored in transport medium and cryopreserved following a standard slow-freezing clinical protocol. Thawed sam...
Source: Cryobiology - December 17, 2023 Category: Biology Authors: Shiyan Tang Celine Jones Jill Davies Sheila Lane Rod T Mitchell Kevin Coward Source Type: research
