Is cryoablation still suitable for advanced non-small cell lung cancer after failure of first-line chemotherapy? A multicenter, prospective, randomized-controlled trial of eighty-seven patients
Cryobiology. 2024 Feb 20:104864. doi: 10.1016/j.cryobiol.2024.104864. Online ahead of print.ABSTRACTThe aim of this study was to investigate the therapeutic effect of cryoablation treatment in advanced NSCLC patients who had failed first-line chemotherapy. Eighty-seven patients from ten hospitals in China were enrolled into the study, forty-four patients received cryoablation treatment plus basic treatment (experimental group), and forty-three patients had basic treatment alone (control group). Follow-up was performed once every three months until the end of the study or the death of the patient. The primary endpoints were...
Source: Cryobiology - February 22, 2024 Category: Biology Authors: Yu Chen Xueni Fang Dan Wang Quanwang Li Kerui Zhang Yuan Li Jinghua Li Haoyue Pang Zhiqiang Cheng Chunyang Zhang Caiyun Zhang Wuwei Yang Baorang Zhu Huanfang Fan Changhui Han Yonghui An Lingling Zhang Baoping Luo Siqi Zhang Taiying Lu Yuanyuan Meng Qinshu Source Type: research
Exploring the relationship between pollen viability and inclusion in Paeonia lactiflora after cryopreservation
Cryobiology. 2024 Feb 20:104867. doi: 10.1016/j.cryobiol.2024.104867. Online ahead of print.ABSTRACTPollen, as the male gametophyte, carries half of plant genetic information and is an important source of germplasm. The cryopreservation of pollen can not only preserve germplasm, but also solve the problem of time and space barrier in crossbreeding. So it is of great significance to explore the mechanism of pollen viability maintenance after cryopreservation. In this paper, 10 cultivars of Paeonia lactiflora with different fresh pollen viability that did not change after cryopreservation were taken as objects and the effect...
Source: Cryobiology - February 22, 2024 Category: Biology Authors: Mengting Zhu Ying Peng Jianzhou Gao Ruifen Ren Yingling Wan Yan Liu Source Type: research
Effect of a magnetic field on droplet freezing and frost formation on cold surfaces
Cryobiology. 2024 Feb 15;114:104866. doi: 10.1016/j.cryobiol.2024.104866. Online ahead of print.ABSTRACTThe effect of a magnetic field on condensed droplet freezing and frost formation was investigated using visualized experimental devices in this study. The size, shape, freezing time of droplets, and frost shape on the magnetic field considerably differ from those on the nonmagnetic surface. Moreover, the magnetic field could suppress droplet freezing and frost formation. The magnetic field suppression effect on droplet freezing and frost formation was analyzed according to the polarity characteristics of water molecules....
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Yujun Gou Yi Qin Jiachun Li Wenbo Zhang Jiacheng Liu Zhe Wang Source Type: research
Cryopreservation of the collector urchin embryo, Tripneustes gratilla
Cryobiology. 2024 Feb 15:104865. doi: 10.1016/j.cryobiol.2024.104865. Online ahead of print.ABSTRACTThe collector urchin, Tripneustes gratilla, is an ecologically important member of the grazing community of Hawaii's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, T. gratilla has also been used as a food source and a biocontrol agent against alien invasive algae species. Due to overexploitation, habitat degradation, and other stressors, their populations face local extirpation. However, artificial reproductive techniques, such as cryopreservation, could provide more consistent seedst...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Charley E Westbrook Jonathan Daly Brian Bowen Mary Hagedorn Source Type: research
Effect of a magnetic field on droplet freezing and frost formation on cold surfaces
Cryobiology. 2024 Feb 15;114:104866. doi: 10.1016/j.cryobiol.2024.104866. Online ahead of print.ABSTRACTThe effect of a magnetic field on condensed droplet freezing and frost formation was investigated using visualized experimental devices in this study. The size, shape, freezing time of droplets, and frost shape on the magnetic field considerably differ from those on the nonmagnetic surface. Moreover, the magnetic field could suppress droplet freezing and frost formation. The magnetic field suppression effect on droplet freezing and frost formation was analyzed according to the polarity characteristics of water molecules....
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Yujun Gou Yi Qin Jiachun Li Wenbo Zhang Jiacheng Liu Zhe Wang Source Type: research
Cryopreservation of the collector urchin embryo, Tripneustes gratilla
Cryobiology. 2024 Feb 15:104865. doi: 10.1016/j.cryobiol.2024.104865. Online ahead of print.ABSTRACTThe collector urchin, Tripneustes gratilla, is an ecologically important member of the grazing community of Hawaii's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, T. gratilla has also been used as a food source and a biocontrol agent against alien invasive algae species. Due to overexploitation, habitat degradation, and other stressors, their populations face local extirpation. However, artificial reproductive techniques, such as cryopreservation, could provide more consistent seedst...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Charley E Westbrook Jonathan Daly Brian Bowen Mary Hagedorn Source Type: research
Effect of a magnetic field on droplet freezing and frost formation on cold surfaces
Cryobiology. 2024 Feb 15:104866. doi: 10.1016/j.cryobiol.2024.104866. Online ahead of print.ABSTRACTThe effect of a magnetic field on condensed droplet freezing and frost formation was investigated using visualized experimental devices in this study. The size, shape, freezing time of droplets, and frost shape on the magnetic field considerably differ from those on the nonmagnetic surface. Moreover, the magnetic field could suppress droplet freezing and frost formation. The magnetic field suppression effect on droplet freezing and frost formation was analyzed according to the polarity characteristics of water molecules.PMID...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Yujun Gou Yi Qin Jiachun Li Wenbo Zhang Jiacheng Liu Zhe Wang Source Type: research
Cryopreservation of the collector urchin embryo, Tripneustes gratilla
Cryobiology. 2024 Feb 15:104865. doi: 10.1016/j.cryobiol.2024.104865. Online ahead of print.ABSTRACTThe collector urchin, Tripneustes gratilla, is an ecologically important member of the grazing community of Hawaii's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, T. gratilla has also been used as a food source and a biocontrol agent against alien invasive algae species. Due to overexploitation, habitat degradation, and other stressors, their populations face local extirpation. However, artificial reproductive techniques, such as cryopreservation, could provide more consistent seedst...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Charley E Westbrook Jonathan Daly Brian Bowen Mary Hagedorn Source Type: research
Effect of a magnetic field on droplet freezing and frost formation on cold surfaces
Cryobiology. 2024 Feb 15:104866. doi: 10.1016/j.cryobiol.2024.104866. Online ahead of print.ABSTRACTThe effect of a magnetic field on condensed droplet freezing and frost formation was investigated using visualized experimental devices in this study. The size, shape, freezing time of droplets, and frost shape on the magnetic field considerably differ from those on the nonmagnetic surface. Moreover, the magnetic field could suppress droplet freezing and frost formation. The magnetic field suppression effect on droplet freezing and frost formation was analyzed according to the polarity characteristics of water molecules.PMID...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Yujun Gou Yi Qin Jiachun Li Wenbo Zhang Jiacheng Liu Zhe Wang Source Type: research
Cryopreservation of the collector urchin embryo, Tripneustes gratilla
Cryobiology. 2024 Feb 15:104865. doi: 10.1016/j.cryobiol.2024.104865. Online ahead of print.ABSTRACTThe collector urchin, Tripneustes gratilla, is an ecologically important member of the grazing community of Hawaii's coral reefs. Beyond its ability to maintain balance between native seaweeds and corals, T. gratilla has also been used as a food source and a biocontrol agent against alien invasive algae species. Due to overexploitation, habitat degradation, and other stressors, their populations face local extirpation. However, artificial reproductive techniques, such as cryopreservation, could provide more consistent seedst...
Source: Cryobiology - February 17, 2024 Category: Biology Authors: Charley E Westbrook Jonathan Daly Brian Bowen Mary Hagedorn Source Type: research
DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
Cryopreservation-induced delayed injury and cell-type-specific responses during the cryopreservation of endothelial cell monolayers
Cryobiology. 2024 Feb 11:104857. doi: 10.1016/j.cryobiol.2024.104857. Online ahead of print.ABSTRACTThe cryopreservation of endothelial cell monolayers is an important step that bridges the cryopreservation of cells in suspension to that of tissues. Previous studies have identified clear distinctions in freezing mechanisms between cells in suspension and in monolayers, as well as developed novel protocols for monolayer cryopreservation. Recently, our group has shown that human umbilical vein endothelial cell (HUVEC) and porcine corneal endothelial cell (PCEC) monolayers grown on Rinzl plastic substrate can be cryopreserved...
Source: Cryobiology - February 13, 2024 Category: Biology Authors: MingHan Yu Leah A Marquez-Curtis Janet A W Elliott Source Type: research
Effects of taurine, cysteine and melatonin as antioxidant supplements to the freezing medium of Prochilodus brevis sperm
This study aimed to test taurine, cysteine, and melatonin on the freezing of Prochilodus brevis sperm and assess its effects on post-thawed sperm quality. Sperm was collected and seven pools were formed (n = 7). They were diluted (1:9) in standard medium (5% glucose, 10% dimethyl sulfoxide and 5% egg yolk) supplemented or not (control) with taurine (0.3, 1.0, 3.16 or 10.0 mM), cysteine (0.3, 1.0, 3.16 or 10.0 mM) or melatonin (0.6, 1.12, 2.0 or 3.56 mM). Post-thawed sperm was evaluated for kinetic (total motility, velocities, and percentage of rapid cells), morphology and membrane and DNA integrity. Differences were found ...
Source: Cryobiology - February 12, 2024 Category: Biology Authors: Thais Maia Torres Priscila Silva de Almeida-Monteiro Renata Vieira do Nascimento Silvio Alencar C ândido-Sobrinho Carla Tatiana Nascimento Sousa Yasmim Maia Ferreira Kamila Teixeira de Paula Carminda Sandra Brito Salmito-Vanderley Source Type: research
