Fed-batch fermentation of Mucor circinelloides reveals significant improvement in biomass and lipid accumulation through performance evaluation, chemical analysis, and expression profiling
This study aimed to improve the lipid and biomass yields of Mucor circinelloides WJ11 by implementing four different fed-batch fermentation strategies, varied in time and glucose concentration (S1-S4). The S1 fermentation strategy yielded the highest biomass, lipid, and fatty acid content (22 ± 0.7 g/L, 53 ± 1.2 %, and 28 ± 1.6 %) after 120 and 144 h, respectively. The γ-linolenic acid titer of 0.75 ± 0.0 g/L was greatest in S3 after 48 h. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to analyze the transcription of key genes involved in lipid accumulation. The glucose-6-phosphate deh...
Source: Bioresource Technology - March 7, 2024 Category: Biotechnology Authors: Hassan Mohamed Tahira Naz Qing Liu Shaoqi Li Xiuwen Wang Yuanda Song Source Type: research
Molecular biology of glucose-6-phosphate dehydrogenase and UDP-glucuronosyltransferase 1A1 in the development of neonatal unconjugated hyperbilirubinemia
Glucose-6-phosphate dehydrogenase (G6PD) deficiency and variants of the UDP-glucuronosyltransferase 1A1 (UGT1A1) gene are the most common genetic causes of neonatal unconjugated hyperbilirubinemia (NUH). In this review, we searched PubMed for articles on the genetic causes of NUH published before December 31, 2022, and analyzed the data. On the basis of the results, we reached eight conclusions: (1) 37 mutations of the G6PD gene are associated with NUH; (2) the clinical manifestation of G6PD deficiency depends not only on ethnicity but also on the molecular mechanisms underlying the deficiency (and thus its severity); (3) ...
Source: Pediatrics and Neonatology - March 3, 2024 Category: Perinatology & Neonatology Authors: Yi-Li Hung, Pi-Feng Chang, Ching-Shan Huang Source Type: research
Molecules, Vol. 29, Pages 1065: Exploration of the Diversity of Vicine and Convicine Derivatives in Faba Bean (Vicia faba L.) Cultivars: Insights from LC-MS/MS Spectra
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Jenny Renaut
While numerous Fabaceae seeds are a good nutritional source of high-quality protein, the use of some species is hampered by toxic effects caused by exposure to metabolites that accumulate in the seeds. One such species is the faba or broad bean (Vicia faba L.), which accumulates vicine and convicine. These two glycoalkaloids cause favism, the breakdown of red blood cells in persons with a glucose-6-phosphate dehydrogenase deficiency. Because this is the most common enzyme deficiency worldwide, faba bean breeding efforts have focused on developing cultivars with low levels of these alkaloids. Consequent...
Source: Molecules - February 29, 2024 Category: Chemistry Authors: Kjell Sergeant Simon Goertz Salma Halime Hanna Tietgen Hanna Heidt Martina Minestrini C édric Jacquard Stephanie Zimmer Jenny Renaut Tags: Article Source Type: research
Molecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes
Parasitol Res. 2024 Feb 29;123(2):146. doi: 10.1007/s00436-024-08155-8.ABSTRACTLeishmaniasis is a neglected disease mainly affecting low-income populations. Conventional treatment involves several side effects, is expensive, and, in addition, protozoa can develop resistance. Photodynamic therapy (PDT) is a promising alternative in treating the disease. PDT involves applying light at a specific wavelength to activate a photosensitive compound (photosensitizer, PS), to produce reactive oxygen species (ROS). Curcumin and its photochemical characteristics make it a good candidate for photodynamic therapy. Studies evaluating ge...
Source: Cell Research - February 28, 2024 Category: Cytology Authors: Luciana Maria Cortez Marcolino Juliana Guerra Pinto Isabelle Ferreira Bruno Henrique Godoi Renata de Azevedo Canevari Juliana Ferreira-Strixino Source Type: research
Molecular effects of photodynamic therapy with curcumin on Leishmania major promastigotes
Parasitol Res. 2024 Feb 29;123(2):146. doi: 10.1007/s00436-024-08155-8.ABSTRACTLeishmaniasis is a neglected disease mainly affecting low-income populations. Conventional treatment involves several side effects, is expensive, and, in addition, protozoa can develop resistance. Photodynamic therapy (PDT) is a promising alternative in treating the disease. PDT involves applying light at a specific wavelength to activate a photosensitive compound (photosensitizer, PS), to produce reactive oxygen species (ROS). Curcumin and its photochemical characteristics make it a good candidate for photodynamic therapy. Studies evaluating ge...
Source: Cancer Control - February 28, 2024 Category: Cancer & Oncology Authors: Luciana Maria Cortez Marcolino Juliana Guerra Pinto Isabelle Ferreira Bruno Henrique Godoi Renata de Azevedo Canevari Juliana Ferreira-Strixino Source Type: research
Functional effects of an African glucose-6-phosphate dehydrogenase (G6PD) polymorphism (Val68Met) on red blood cell hemolytic propensity and post-transfusion recovery
DISCUSSION: This novel mouse model of the common G6PD A- variant has impaired antioxidant capacity like humans and low G6PD activity may reduce survival of transfused RBCs when irradiation is performed.PMID:38400625 | DOI:10.1111/trf.17756 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Ling Wang Elizabeth R Rochon Sebastien Gingras Benjamin E Zuchelkowski Derek J Sinchar Elimira Alipour Julie A Reisz Minying Yang Grier P Page Tamir Kanias Darrell J Triulzi Janet S Lee Daniel B Kim-Shapiro Angelo D'Alessandro Mark T Gladwin Source Type: research
Functional effects of an African glucose-6-phosphate dehydrogenase (G6PD) polymorphism (Val68Met) on red blood cell hemolytic propensity and post-transfusion recovery
DISCUSSION: This novel mouse model of the common G6PD A- variant has impaired antioxidant capacity like humans and low G6PD activity may reduce survival of transfused RBCs when irradiation is performed.PMID:38400625 | DOI:10.1111/trf.17756 (Source: Transfusion)
Source: Transfusion - February 24, 2024 Category: Hematology Authors: Ling Wang Elizabeth R Rochon Sebastien Gingras Benjamin E Zuchelkowski Derek J Sinchar Elimira Alipour Julie A Reisz Minying Yang Grier P Page Tamir Kanias Darrell J Triulzi Janet S Lee Daniel B Kim-Shapiro Angelo D'Alessandro Mark T Gladwin Source Type: research
Radical cure for Plasmodium vivax malaria after G6PD qualitative testing in four provinces in Cambodia, results from Phase I implementation
Cambodia aims to eliminate all forms of malaria by 2025. In 2020, 90% of all malaria cases were Plasmodium vivax. Thus, preventing P. vivax and relapse malaria is a top priority for elimination. 14-day primaquine... (Source: Malaria Journal)
Source: Malaria Journal - February 23, 2024 Category: Infectious Diseases Authors: Dysoley Lek, Yu-Cheng Tsai, Jillian Hirano, Siv Sovannaroth, Voeurng Bunreth, Prak Vonn, Or Vannthen, Tol Bunkea, Top Samphornarann, Nguon Sokomar, Mak Sarath, Soy Ty Kheang, Evelyn Wong, Michelle K. Burbach, Jayme Hughes and Huy Rekol Tags: Research Source Type: research
Prednisone and prednisolone effects on development, blood, biochemical and histopathological markers of Aquarana catesbeianus tadpoles
Aquat Toxicol. 2024 Feb 18;268:106869. doi: 10.1016/j.aquatox.2024.106869. Online ahead of print.ABSTRACTSynthetic glucocorticoids are often found in surface waters and can cause harmful effects to aquatic organisms such as amphibians. In this work we evaluated the effects of the drugs prednisone (PD) and prednisolone (PL) on developmental, molecular, blood, biochemical and histological markers. Aquarana catesbeianus tadpoles were exposed for 16 days to environmentally relevant concentrations of 0, 0.1, 1 and 10 µg/L of both drugs. PD increased the transcript levels of the enzyme deiodinase III (Dio3), the hormones cortis...
Source: Aquatic Toxicology - February 22, 2024 Category: Toxicology Authors: Camila Fatima Rutkoski Suelen Cristina Grott Nicole Grasmuk Israel Fernando de Campos Guerreiro Francisco Estev ão Carneiro Daiane Bitschinski Aline Warsneski Priscila Aparecida Horn Da ína Lima Camila Lisarb Velasquez Bastolla Jac ó Joaquim Mattos Afo Source Type: research
Prednisone and prednisolone effects on development, blood, biochemical and histopathological markers of Aquarana catesbeianus tadpoles
Aquat Toxicol. 2024 Feb 18;268:106869. doi: 10.1016/j.aquatox.2024.106869. Online ahead of print.ABSTRACTSynthetic glucocorticoids are often found in surface waters and can cause harmful effects to aquatic organisms such as amphibians. In this work we evaluated the effects of the drugs prednisone (PD) and prednisolone (PL) on developmental, molecular, blood, biochemical and histological markers. Aquarana catesbeianus tadpoles were exposed for 16 days to environmentally relevant concentrations of 0, 0.1, 1 and 10 µg/L of both drugs. PD increased the transcript levels of the enzyme deiodinase III (Dio3), the hormones cortis...
Source: Aquatic Toxicology - February 22, 2024 Category: Toxicology Authors: Camila Fatima Rutkoski Suelen Cristina Grott Nicole Grasmuk Israel Fernando de Campos Guerreiro Francisco Estev ão Carneiro Daiane Bitschinski Aline Warsneski Priscila Aparecida Horn Da ína Lima Camila Lisarb Velasquez Bastolla Jac ó Joaquim Mattos Afo Source Type: research
Construction of an Oxidative Stress Risk Model to Analyze the Correlation Between Liver Cancer and Tumor Immunity
CONCLUSION: The combined assessment of EZH2, ANKZF1, G6PD, and HMOX1 gene expression can serve as a model to evaluate the risk of oxidative stress in HCC. Furthermore, there is a notable correlation between the expression of these risk model genes and tumor immunity.PMID:38375834 | DOI:10.2174/0115680096284532231220061048 (Source: Current Cancer Drug Targets)
Source: Current Cancer Drug Targets - February 20, 2024 Category: Cancer & Oncology Authors: Ying Liu Yufeng Li Li Chen Weina Zha Jing Zhang Kun Wang Chunhai Hao Jianhe Gan Source Type: research
Construction of an Oxidative Stress Risk Model to Analyze the Correlation Between Liver Cancer and Tumor Immunity
CONCLUSION: The combined assessment of EZH2, ANKZF1, G6PD, and HMOX1 gene expression can serve as a model to evaluate the risk of oxidative stress in HCC. Furthermore, there is a notable correlation between the expression of these risk model genes and tumor immunity.PMID:38375834 | DOI:10.2174/0115680096284532231220061048 (Source: Current Cancer Drug Targets)
Source: Current Cancer Drug Targets - February 20, 2024 Category: Cancer & Oncology Authors: Ying Liu Yufeng Li Li Chen Weina Zha Jing Zhang Kun Wang Chunhai Hao Jianhe Gan Source Type: research
Newborn screening for congenital hypothyroidism, congenital adrenal hyperplasia and glucose-6-phosphate dehydrogenase deficiency in Bihar: A pressing priority in today's time
CONCLUSIONS: Preliminary data on the prevalence of various genetic disorders revealed that CAH is the most prevalent disorder followed by CH in the population of Bihar. More efforts need to be undertaken to create awareness and to make screening a successful programme in India. A cost-effective nationwide screening programme is highly recommended for the detection of such cases at the earliest to avoid their future complication.PMID:38361884 | PMC:PMC10866230 | DOI:10.4103/jfmpc.jfmpc_1029_23 (Source: Primary Care)
Source: Primary Care - February 16, 2024 Category: Primary Care Authors: Bandana Kumari Khushboo Raj Sadhana Sharma Sushil Kumar Bhabesh K Chowdhry Amit Kumar Source Type: research
Newborn screening for congenital hypothyroidism, congenital adrenal hyperplasia and glucose-6-phosphate dehydrogenase deficiency in Bihar: A pressing priority in today's time
CONCLUSIONS: Preliminary data on the prevalence of various genetic disorders revealed that CAH is the most prevalent disorder followed by CH in the population of Bihar. More efforts need to be undertaken to create awareness and to make screening a successful programme in India. A cost-effective nationwide screening programme is highly recommended for the detection of such cases at the earliest to avoid their future complication.PMID:38361884 | PMC:PMC10866230 | DOI:10.4103/jfmpc.jfmpc_1029_23 (Source: Primary Care)
Source: Primary Care - February 16, 2024 Category: Primary Care Authors: Bandana Kumari Khushboo Raj Sadhana Sharma Sushil Kumar Bhabesh K Chowdhry Amit Kumar Source Type: research
DNA fragmentation, antioxidant activity and histological structure of cryopreserved testicular tissue depending on sexual maturity and immunological status
The objective of this work was to determine a relationship between a frequency of DNA fragmentation, a level of antioxidant activity and a preservation of histological structure depending on initial status of fragments of seminiferous tubules of testes (FSTT) of rats at the stages of cryopreservation. FSTT of animals of different ages (immature, mature), as well as animals with changed immunological status (adjuvant arthritis) were cryopreserved. Slow uncontrolled freezing was used in a cryomedium of fibrin gel with 0.7 M glycerol. The results showed that viability, TAS, γGGT and G6PD activities had the highest values in ...
Source: Cryobiology - February 15, 2024 Category: Biology Authors: Nataliia Volkova Mariia Yukhta Anatoliy Goltsev Source Type: research
