Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research
Glucose and redox metabolism in meiotically blocked in vitro grown mouse antral follicles
ConclusionsCurrent results suggest that in addition to impaired nuclear maturation, metabolic disruption is present in MB follicles. MB follicles either compensate with high levels of TCA cycle and PPP activities in CCs, or are unable to drive proper levels of aerobic metabolism, which might be due to the current culture conditions. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - November 18, 2023 Category: Reproduction Medicine Source Type: research
Insights on epithelial cells at the single-cell level in hepatocellular carcinoma prognosis and response to chemotherapy
Conclusion: This study revealed the cellular composition of HCC, the differentiation evolution and functional landscape of epithelial cells in the further deterioration of HCC, and established a 3-gene risk model, which was closely related to clinical features, EMT, and drug sensitivity prediction. These findings provided insights in patient prognosis and drug therapy detection for HCC. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - November 17, 2023 Category: Drugs & Pharmacology Source Type: research
G6pdh as a key immunometabolic and redox trigger in arthropods
The enzyme glucose-6-phosphate dehydrogenase (G6PDH) plays crucial roles in glucose homeostasis and the pentose phosphate pathway (PPP), being also involved in redox metabolism. The PPP is an important metabolic pathway that produces ribose and nicotinamide adenine dinucleotide phosphate (NADPH), which are essential for several physiologic and biochemical processes, such as the synthesis of fatty acids and nucleic acids. As a rate-limiting step in PPP, G6PDH is a highly conserved enzyme and its deficiency can lead to severe consequences for the organism, in particular for cell growth. Insufficient G6PDH activity can lead t...
Source: Frontiers in Physiology - November 17, 2023 Category: Physiology Source Type: research
The enzymes of the oxidative phase of the pentose phosphate pathway as targets of reactive species: consequences for NADPH production
Biochem Soc Trans. 2023 Nov 16:BST20231027. doi: 10.1042/BST20231027. Online ahead of print.ABSTRACTThe pentose phosphate pathway (PPP) is a key metabolic pathway. The oxidative phase of this process involves three reactions catalyzed by glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL) and 6-phosphogluconate dehydrogenase (6PGDH) enzymes. The first and third steps (catalyzed by G6PDH and 6PGDH, respectively) are responsible for generating reduced nicotinamide adenine dinucleotide phosphate (NAPDH), a key cofactor for maintaining the reducing power of cells and detoxification of both endogenous an...
Source: Biochemical Society Transactions - November 16, 2023 Category: Biochemistry Authors: Eduardo Fuentes-Lemus Juan Sebasti án Reyes Juan David Figueroa Michael J Davies Camilo L ópez-Alarcón Source Type: research
First Insight into Drug Resistance Genetic Markers, Glucose-6-Phosphate Dehydrogenase and Phylogenetic Patterns of Misdiagnosed Plasmodium vivax Malaria in Far North Region, Cameroon
This study aimed at characterizing drug resistance genes, glucose-6-phosphate dehydrogenase gene (G6PD), and phylogenetic patterns of a Pv + Pf co-infection misdiagnosed as a Pf mono-infection in the Far North region of Cameroon. Only one non-synonymous mutation in the pvdhps gene A383G was found. Pv drug resistance gene sequences were phylogenetically closer to the reference SAL-I strain and isolates from Southeast Asia and Western Pacific countries. Analyzing co-infecting Pf revealed no resistance mutations in Pfmdr1 and Pfk13 genes, but mutations in Pfcrt (C72V73I74E75T76) and Pfdhfr-Pfdhps genes (A16C50I51R59N108L164 -...
Source: Current Microbiology - November 15, 2023 Category: Microbiology Authors: Loick Pradel Kojom Foko Jahnvi Jakhan Suman Tamang Joseph Hawadak Francine Dorgelesse Kouemo Motse Vineeta Singh Source Type: research
First Insight into Drug Resistance Genetic Markers, Glucose-6-Phosphate Dehydrogenase and Phylogenetic Patterns of Misdiagnosed Plasmodium vivax Malaria in Far North Region, Cameroon
This study aimed at characterizing drug resistance genes, glucose-6-phosphate dehydrogenase gene (G6PD), and phylogenetic patterns of a Pv + Pf co-infection misdiagnosed as a Pf mono-infection in the Far North region of Cameroon. Only one non-synonymous mutation in the pvdhps gene A383G was found. Pv drug resistance gene sequences were phylogenetically closer to the reference SAL-I strain and isolates from Southeast Asia and Western Pacific countries. Analyzing co-infecting Pf revealed no resistance mutations in Pfmdr1 and Pfk13 genes, but mutations in Pfcrt (C72V73I74E75T76) and Pfdhfr-Pfdhps genes (A16C50I51R59N108L164 -...
Source: Current Microbiology - November 15, 2023 Category: Microbiology Authors: Loick Pradel Kojom Foko Jahnvi Jakhan Suman Tamang Joseph Hawadak Francine Dorgelesse Kouemo Motse Vineeta Singh Source Type: research
Molecular characterization of < i > G6PD < /i > mutations identifies new mutations and a high frequency of intronic variants in Thai females
This study aimed to develop multiplex high-resolution melting (HRM) analyses to enable the accurate detection ofG6PD mutations, especially among females with heterozygous deficiency. Multiplex HRM assays were developed to detect sixG6PD variants, i.e.,G6PD Gaohe (c.95A>G),G6PD Chinese-4 (c.392G>T),G6PD Mahidol (c.487G>A),G6PD Viangchan (c.871G>A),G6PD Chinese-5 (c.1024C>T), andG6PD Union (c.1360C>T) in two reactions. The assays were validated and then applied to genotypeG6PD mutations in 248 Thai females. The sensitivity of the HRM assays developed was 100% [95% confidence interval (CI): 94.40% –100%] with a specificity ...
Source: PLoS One - November 15, 2023 Category: Biomedical Science Authors: Kamonwan Chamchoy Source Type: research
Fungicides from rice cultivation (tebuconazole and azoxystrobin) alters biochemical and histological markers of hammertoad tadpoles (Boanafaber)
Environ Pollut. 2023 Nov 10:122900. doi: 10.1016/j.envpol.2023.122900. Online ahead of print.ABSTRACTTebuconazole (TBZ) and azoxystrobin (AZX) are fungicides frequently used in rice cultivation. Despite protecting crops against fungal diseases, these compounds can contaminate the natural environments close to the crops, exerting negative effects on non-target organisms, the present study aimed to characterize the contamination by fungicides of a river that flows in an area dominated by rice cultivation in the north of the state of Santa Catarina, SC, Brazil. Concentrations of TBZ and AZX found in the field were used to eva...
Source: Environmental Pollution - November 12, 2023 Category: Environmental Health Authors: Aline Warsneski Daiane Bitschinski Camila F átima Rutkoski Nicole Grasmuk Israel Gustavo Henrique Pereira Gon çalves Luiza L ã Fernando Guerreiro Lu ís Olímpio Menta Giasson Cl áudia Almeida Coelho de Albuquerque Rony Paolin Hasckel Elizia Barbosa d Source Type: research
