About the article "Recommendations for the application and the follow-up of quality controls in medical biology laboratories" Ann Biol Clin 2019  ; 77(5) : 577-97.
About the article "Recommendations for the application and the follow-up of quality controls in medical biology laboratories" Ann Biol Clin 2019 ; 77(5) : 577-97. Ann Biol Clin (Paris). 2020 Jan 08;: Authors: Sobas F PMID: 31916539 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - January 8, 2020 Category: Biochemistry Authors: Sobas F Tags: Ann Biol Clin (Paris) Source Type: research

Adequate prescription of medical biology exams: what perspective of it's valorization?
PMID: 31908316 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - January 7, 2020 Category: Biochemistry Authors: Abraham S, Henry A, Amara M, Juillet SM Tags: Ann Biol Clin (Paris) Source Type: research

Pregnancy in metabolic diseases with hepatic expression: what risks for the mother and the child?
in AF Abstract Inborn errors of metabolism (IEM) are rare diseases caused by mutations in genes encoding enzymes or carriers. Qualitative or quantitative protein deficiency induces both an accumulation of precursor metabolites and a lack of products downstream of the blockade. Pregnancy in patients with IEM is a condition likely to promote metabolic decompensation. In this review, we presented liver symptoms described during pregnancy in a context of hepatic IEM. In particular, we detailed clinical and biological abnormalities specifically occurring in tyrosinemia type I, Wilson disease, and main urea cycle defect...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Moreau C, Joueidi Y, Peoc'h K, Bardou-Jacquet E, Le Lous M, Bendavid C, Lavoué V, Damaj L, Dessein AF Tags: Ann Biol Clin (Paris) Source Type: research

Development of molecular analyzes by digital PCR for clinical practice: positioning, current applications and perspectives.
Abstract This review is the second part of the workshop on digital PCR (dPCR) proposed by the working group of the French society of clinical biology. The first part of the paper discusses the advantages and limitations of dPCR for the search of different molecular abnormalities such as point mutations, copy number variants, DNA methylation, RNA analysis and a more innovative application, the single-cell dPCR. This synthesis makes it possible to propose a positioning of the dPCR compared to the other available technologies in a medical laboratory. In a second part, the main current applications of the dPCR will be...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Denis JA, Perrier A, Nectoux J, Lamy PJ, Alary AS, Sarafan-Vasseur N, Henaff D, Busser B, Appay R, Pedini P, Romanet P, Taly V, Fina F Tags: Ann Biol Clin (Paris) Source Type: research

Phosphatidylethanol blood analysis.
This article aims to place the phosphatidylethanol (PEth) blood test in the detection area of ethanol consumption causing alcohol-related disorders, to present the current methods of analysis, data on interpretation, some practical applications and the prospects of use of this biomarker. PEth is a minor metabolite of ethanol. Among nearly 50 PEth counterparts, PEth 16:0/18:1 is the most abundant. The interest that PEth brings compared to other biomarkers is the extended window of detection of ethanol consumptions. Indeed, it has a blood elimination half-life of approximately 5 days, which offers estimated alcohol consumpti...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Hakim F, Wiart JF, Ménard O, Allorge D, Gaulier JM Tags: Ann Biol Clin (Paris) Source Type: research

Performance analysis of the Sysmex UF4000/UD10 for diagnosis of urinary tract infections.
CONCLUSION: the correlation UF4000/microscopy is satisfactory for cellularities, the UD10 allowing to correct discrepancies. The «GN?» flag is reliable allowing a quick diagnostic orientation for the clinician. Finally, UF4000/UD10 has shown very good performances, notably thanks to the integration of the UD-10 image analyzer, which eliminates time consuming optical microscopy cell count. PMID: 31859641 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Allain M, Sun K, Predal C, Nassif X, Ferroni A Tags: Ann Biol Clin (Paris) Source Type: research

Macrophage activation syndrome revealing colon carcinoma About a case.
We report here a case of MAS that revealed a colon carcinoma with medullary metastasis in a 62 years old patient. MAS can complicate or reveal a solid tumor in only 1.6% of cases, especially when there is spinal metastasis. This association has rarely been reported in the literature, and only in isolated cases. Moreover, in our patient reports the metastases of colon cancer are located in the bone, which is less frequent than liver and lung metastases. MAS is a serious pathology with high mortality, associated with inappropriate activation of the immune system. Etiological treatment is necessary and may be sufficient. ...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Amaddah R, Aznag M, Boufrioua EG, Yahyaoui H, Bahri R, Raissi A, Ait Ameur M, Chakour M Tags: Ann Biol Clin (Paris) Source Type: research

False-positive of blood culture instrument: leukocytosis, overfilled vials or defective position?
Abstract Continuous monitoring of the performances of blood culture instrument can be based on the indicators proposed by the QUAMIC. Of these, the analytic performance indicator evaluates the rate of false-positive vials. False-positives vials can be reported by the device in case of leukocytosis or when vials are overfilled. In our laboratory, we record prospectively in our software all false positive vials as well as the position used for their incubation. These data are analyzed at least twice a year. For the first half of 2016, this strategy allowed us to identify a defective position. We then evaluated the i...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Pitsch A, Ergani A, Vasse M, Farfour E Tags: Ann Biol Clin (Paris) Source Type: research

Stability study and external quality assessment scheme implementation for complement components dosage on EDTA plasma.
Abstract Although the use of EDTA-containing collection tubes is known to stabilize the complement analytes and to make the results more reliable, no external quality assessment (EQA) scheme based on EDTA plasma samples is available to date in France. Consequently, a number of clinical laboratories currently participate to EQA program on samples whose matrix is different from their routine practice. The aim of this work was to offer a new external quality assessment scheme, as an inter-laboratory exchange (ILE). The ILE samples come from pooled EDTA plasmas of healthy subjects and are diluted to obtain distinct co...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Lopez B, Rogeau S, Deleplancque AS, Moitrot E, Berthe E, Goulvestre C, Hillion S, Vinner E, Labalette M, Dubucquoi S Tags: Ann Biol Clin (Paris) Source Type: research

Accreditation strategy for rare somatic molecular abnormalities detected or quantified by polymerase chain reaction: GBMHM recommendations.
le Challenge 180 secondes Abstract In 2020, accreditation of molecular tests according to ISO 15189 is a requirement for all French medical laboratories. For many years, the GBMHM group (French Group of Molecular Biologists in Hematology) supports this approach through organization of external quality evaluation campaigns, and by publishing recommendations that have allowed the accreditation of the most frequent molecular tests for most laboratories. However, some molecular abnormalities concerns very few patients (and sometimes a single patient), and therefore cannot be evaluated in the same way, because of the l...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Sujobert P, Dulucq S, Alary AS, Etancelin P, Bouvier A, Boureau L, Chauveau A, Kosmider O, Flandrin P, Pour l’ensemble des auteurs de la session « Posters » sélectionnés pour le Challenge 180 secondes Tags: Ann Biol Clin (Paris) Source Type: research

Beware of the dog! Septic shock due to Capnocytophaga canimorsus revealed on peripheral blood smear.
Abstract A blood smear examination led to the diagnosis of severe bacterial infection in a splenectomized patient. The knowledge of a relevant clinical information (recent dog bite) could have led to immediate presumptive identification of the causative agent, Capnocytophaga canimorsus. PMID: 31859646 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Gosset F, Sarret B, Mortreux S, Moquet O Tags: Ann Biol Clin (Paris) Source Type: research

Review of the sweat test indications in a Brussels' cystic fibrosis reference center.
Abstract Sweat test is the gold standard of the diagnosis of cystic fibrosis (CF). The aim of our study was to identify the indications leading to perform a sweat test and those that led to the diagnosis of CF. METHODOLOGY: We collected data of all sweat tests performed between 2008, 1th of March and 2015, 28th of February. They were analyzed following Rosenstein diagnosis criteria (1998): clinical manifestations suggesting CF, positive neonatal screening (≥ 1 positive assay of immunoreactive trypsin) or familial history of CF. RESULTS: We reviewed 1,208 sweat tests over this period. Patients were age...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Bensliman S, Lefevre N, Duchateau J, Hanssens L Tags: Ann Biol Clin (Paris) Source Type: research

Biotin analytical interference on Siemens immunoanalysis on Dimension EXL ®: impact on cardiac troponin I and total vitamin D (D2+D3) assays.
Biotin analytical interference on Siemens immunoanalysis on Dimension EXL®: impact on cardiac troponin I and total vitamin D (D2+D3) assays. Ann Biol Clin (Paris). 2019 Dec 01;77(6):693-696 Authors: Djebrani M, Jabbour F, Hulot FD, De Mazancourt P Abstract Several hyperthyroidism misdiagnoses cases have been recently described due to biotin intake. Biotin used in immuno-analysis assays which rely on biotin/streptavidin binding properties. In these assays, high plasmatic biotin levels can lead to major analytical interferences resulting in falsely higher (competition tests) or falsely reduced deter...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Djebrani M, Jabbour F, Hulot FD, De Mazancourt P Tags: Ann Biol Clin (Paris) Source Type: research

Branch of Biologie Sans Fronti ères Ile-de-France area: activities and prospects.
Branch of Biologie Sans Frontières Ile-de-France area: activities and prospects. Ann Biol Clin (Paris). 2019 Dec 01;77(6):697-699 Authors: Pangon B, Salsi H, Cals MJ PMID: 31859649 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Pangon B, Salsi H, Cals MJ Tags: Ann Biol Clin (Paris) Source Type: research

Oral communications from the 3e Congr ès de biologie praticienne et 4e Congrès de médecine de laboratoire, Fédération internationale francophone de biologie clinique et de médecine de laboratoire (FIFBCML), Alger, octobre 2019.
Oral communications from the 3e Congrès de biologie praticienne et 4e Congrès de médecine de laboratoire, Fédération internationale francophone de biologie clinique et de médecine de laboratoire (FIFBCML), Alger, octobre 2019. Ann Biol Clin (Paris). 2019 Dec 01;77(6):701-711 Authors: Achir I, Amari H, Arrache D, Baghdali FY, Bitam A, Blanchecotte F, Chabati O, Chachou A, Djenane A, Djenane F, Djenouhat K, Djidjik R, Ferahta I, Gagi N, Gharnaout M, Ghaffour M, Guettouche S, Gruson D, Haddad C, Haddoum F, Hasni M, Hedhili A, Kheloui Y, Kraiba R, Lacroix I, Lamani A, Lara...
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Achir I, Amari H, Arrache D, Baghdali FY, Bitam A, Blanchecotte F, Chabati O, Chachou A, Djenane A, Djenane F, Djenouhat K, Djidjik R, Ferahta I, Gagi N, Gharnaout M, Ghaffour M, Guettouche S, Gruson D, Haddad C, Haddoum F, Hasni M, Hedhili A, Kheloui Y, Tags: Ann Biol Clin (Paris) Source Type: research

Reports of the Match 180 seconds from the third French-speaking Days of Medical Biology, Monaco, 2019.
PMID: 31859651 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Authors: Tournoys MH, Poupon C, Guillerme J, David A, Malaure C, Heng E, Horikian A, Tira M, Rambaud A Tags: Ann Biol Clin (Paris) Source Type: research

Lu pour vous dans ….
Lu pour vous dans…. Ann Biol Clin (Paris). 2019 Dec 01;77(6):718-720 Authors: PMID: 31859652 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - December 1, 2019 Category: Biochemistry Tags: Ann Biol Clin (Paris) Source Type: research

Thanatobiochemistry: its contribution to the diagnosis of hypothermia fatalities.
ute;rolle C Abstract Hypothermia is a potentially lethal pathology whose in vivo diagnosis is relatively easy, based on the observation of a body temperature below 35̊C. However, its post mortem diagnosis is much more complex because of a thermal decrease occurring systematically after the death, which renders most often uninterpretable the body temperature as a diagnostic argument for hypothermia. Moreover, none of the macroscopic or microscopic autopsy elements described as suspicious of hypothermia are pathognomonic of a hypothermic death. Currently, this diagnosis arises in the light of an association of conc...
Source: Annales de Biologie Clinique - October 21, 2019 Category: Biochemistry Authors: Rousseau G, Rougé-Maillart C, Alarcan H, Drevin G, Gaconnet A, Reynier P, Saint-Martin P, Rérolle C Tags: Ann Biol Clin (Paris) Source Type: research

Metals: common clinical applications in inductively coupled plasma mass spectrometry.
c'h K Abstract Inductively coupled plasma mass spectrometry (ICP-MS) is an analytical technique for inorganic, quantitative, and multi-elemental compounds commonly used in clinical biology. In recent years, it has gradually replaced atomic absorption techniques. It is based on the combination of an ion source formed of an inductively coupled plasma torch by a high-frequency electromagnetic generator, and a mass spectrometer, which allows the separation of ions generated according to their mass and energy. It allows the simultaneous detection of most metal and metalloid elements (about 80, 40 generally). It is extr...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Cavey T, Ropert M, Loréal O, Bendavid C, Peoc'h K Tags: Ann Biol Clin (Paris) Source Type: research

NSE & S100B protein blood level assessment during a long-distance trail race.
NSE & S100B protein blood level assessment during a long-distance trail race. Ann Biol Clin (Paris). 2019 Oct 01;77(5):532-536 Authors: Jouffroy R, Alves B, Mauvieux B, Mallet L, Beaudeux JL, Cottart CH Abstract The acute and chronic consequences of long-distance running on brain function have received little attention. The impact of such a hard-physical burden associated with sleep privation during such events such has never been explored in terms of neuropsychological function and brain damage. METHODS: Blood samples were collected from 4 athletes before, during and at the end of one of two...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Jouffroy R, Alves B, Mauvieux B, Mallet L, Beaudeux JL, Cottart CH Tags: Ann Biol Clin (Paris) Source Type: research

A flowchart is always useful: application to the interpretation of the cytobacteriological examination of urine.
The objectives of this work were to present our experience in carrying out the CBEU and to share one of the means that we consider very useful in the daily practice of this crucial analysis. This is a 28-month prospective study (March 2016 to June 2018). The CBEU was carried out in accordance with the recommendations of the medical microbiology referential (REMIC). Antibiotic susceptibility was studied in accordance with the recommendations of the l'European committee on antimicrobial susceptibility testing (EUCAST). Cultures were positive for urinary tract infection in 5.09% (n=769) of cases and for colonization in 4.88% ...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Maleb A, Lamrabat S, Rifai S, Rahmani N, Bensalah M, Benaissa E, Lahlou YB, Frikh M, Elouennass M Tags: Ann Biol Clin (Paris) Source Type: research

Positive HBs antigen in the absence of hepatitis B virus infection.
let J Abstract French recommendations for the screening of hepatitis B virus (HBV) infection were updated in 2019 with the association of three markers: HBs Ag, anti-HBs Ab and anti-HBc Ab. These three markers allow identification of infected patients, vaccinated patients and patients who have been in contact with HBV. A positive HBs Ag is usually associated with HBV infection but this interpretation must take into account the clinical context. In particular, the absence of anti-HBc Ab, normal ALAT levels and the absence of jaundice can be associated with recent HBV vaccination or false-positive HBs Ag. Recent HBV...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Cachinho JB, François M, Stefic K, Marlet J Tags: Ann Biol Clin (Paris) Source Type: research

Difficile examen direct par coloration de Gram : à propos d'un cas d'endocardite à Cardiobacterium hominis et revue de la littérature.
Difficile examen direct par coloration de Gram : à propos d'un cas d'endocardite à Cardiobacterium hominis et revue de la littérature. Ann Biol Clin (Paris). 2019 Oct 01;77(5):549-556 Authors: Blanchot T, Muggeo A, Limelette A, Abdelaziz A, Brasme L, Quinquenel A, Vernet-Garnier V, Delmer A, de Champs C, Guillard T Abstract Cardiobacterium hominis est un bacille à Gram négatif responsable d'endocardites infectieuses, principalement chez les patients atteints de pathologies cardiaques ou porteurs de valves. L'identification de cette bactérie est souvent complex...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Blanchot T, Muggeo A, Limelette A, Abdelaziz A, Brasme L, Quinquenel A, Vernet-Garnier V, Delmer A, de Champs C, Guillard T Tags: Ann Biol Clin (Paris) Source Type: research

Microbiological testing of flexible endoscopes: contribution of the approach of accreditation in the implementation of regulation.
We presented the assay at the COFRAC certification, to ensure a maximal level of confidence in the quality of our results. The existing quality system management was used by the type B widened flexible scope for data from previous accreditations for the identification of Staphylococcus aureus, Enterococci and Enterobacteriaceae. The quality insurance of the results and the technical authorization were guaranteed by the participation at a program of interlaboratory comparisons and the elaboration of internal quality controls. Risks analyses and conventions were wrote with endoscopy sectors. The visit led the opening of a si...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Lecointe D, Sevin T, Gouot A, Assoukpa J, Kassidi N Tags: Ann Biol Clin (Paris) Source Type: research

Recommendations for the application and the follow-up of quality controls in medical biology laboratories.
Abstract The recommendations that we formulate in this document come from LABAC, SFBC and FAEEQ. They describe the different steps from the initial application of quality controls, based on risk analysis: the changes of reagent batches or internal quality controls (IQC) batches, the course when IQC are not in accordance with references, the choice of external quality evaluation and the interpretation of its results, the comparability of results obtained in several analysers used in the same laboratory. Lastly, measurement uncertainty, robustness of methods and specificities of near-patient biology and rapid tests ...
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Authors: Giannoli JM, Albarede S, Avellan T, Bouilloux JP, Cartier R, Cohen R, Colard N, Essemilaire L, Galinier JL, Kuentz M, Paris M, Portugal H, Scherrer F, Siest JP, Vassault A, Vialle JM Tags: Ann Biol Clin (Paris) Source Type: research

Lu pour vous dans ….
Lu pour vous dans…. Ann Biol Clin (Paris). 2019 Oct 01;77(5):599-600 Authors: PMID: 31638590 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - October 1, 2019 Category: Biochemistry Tags: Ann Biol Clin (Paris) Source Type: research

Optimization of incubation duration of culture media in microbiology.
Abstract In order to perform biological analysis, clinical laboratories apply the instructions of reagent suppliers. For culture media these instructions are often incomplete and poorly adapted to the variety of clinical samples and micro-organisms. The REMIC can help to overcome these shortcomings. Required time of incubation for culture media are proposed based on the nature of the sample and the type of micro-organism suspected. Nevertheless, they are most often expressed in multiple of 24 hours and they are often considered as minimal by the laboratories. As the samples are inoculated "continuously",...
Source: Annales de Biologie Clinique - September 11, 2019 Category: Biochemistry Authors: Farfour E, Limousin L, Henry A, Cardot E, Cahen P, Lecointe D, Jolly E, Vasse M, Mathonnet D Tags: Ann Biol Clin (Paris) Source Type: research

Y chromosome microdeletions screening in Tunisian infertile men.
Abstract The aim of this study was to establish the prevalence of chromosomal abnormalities and microdeletions on the Y chromosome in Tunisian infertile men with severe oligozoospermia or non-obstructive azoospermia. In cases of azoospermia, we aimed also to correlate histological results after negative testicular sperm extraction with the type of Y chromosome microdeletion. 84 infertile patients and 52 controls were screened for karyotypic abnormalities using G-banding and Yq chromosome microdeletions using multiplex PCR. 7 infertile males (8.3%) carried chromosomal abnormalities and 8 (9.5%) presented Y chromoso...
Source: Annales de Biologie Clinique - September 11, 2019 Category: Biochemistry Authors: Chabchoub I, Kdous M, Zhioua F, Gaied A, Merdassi G Tags: Ann Biol Clin (Paris) Source Type: research

A primitive plasma cell leukemia with immunoglobulin (Ig) E.
We report here a case of primitive plasma cell leukemia with immunoglobulin (Ig) E. IgE myeloma is an exceptional variant of multiple myeloma, with a very poor prognosis. Its biological diagnosis requires specific analyzes in order to detect IgE gammopathy. Plasma cell leukemia (PCL) is also a very rare and very severe form of multiple myeloma. There are two variants: primitive PCL (pPCL) occurring de novo and secondary PCL (sPCL), evolution of a preexisting myeloma. Its diagnosis is essentially biological since it is defined by a blood plasmocytosis greater than 2 G/L or 20% of the leucocytes. PMID: 31512579 [PubMed ...
Source: Annales de Biologie Clinique - September 11, 2019 Category: Biochemistry Authors: Rachidi M, Lazarian G, Letestu R, Lusina D, Desbene C, Vidal V, Eclache V, Baran-Marszak F, Cymbalista F, Fleury C Tags: Ann Biol Clin (Paris) Source Type: research

Diagnostic workup in front of an adult monocytosis.
Abstract The discovery of a monocytosis is a frequent phenomenon, requiring confirmation by reading under a microscope by an experimented biologist, to overcome usual cytological traps such as the presence of hairy cells, promonocytes or monoblasts. In the vast majority of cases the secondary origin is very easily found by the context and/or the presence of a biological inflammatory syndrome. More rarely the diagnosis is directed towards an eosinophilic pathology or an acute leukemia. In other cases, CMML, MPN or MDS with monocytosis may be highlighted. In the absence of any pathognomonic element and the presence ...
Source: Annales de Biologie Clinique - September 4, 2019 Category: Biochemistry Authors: Labrousse J, Pasini S, Vignon G, Bonnin A, Mottaz P, Carrere F, Augereau PF, Aucher P, Lellouche F Tags: Ann Biol Clin (Paris) Source Type: research

Could the publication of new european guidelines change practices in France?
ine A Abstract The use of predictive biomarkers in the diagnosis and prediction of the efficacy of targeted therapies for the individualized management of patients is generally based on the use of in vitro medical diagnosis devices that are now covered by the guidelines 90/385/EEC, 93/42/EEC and 98/42/EEC. On 25 May 2017, the European Parliament and Council Regulations 2017/745 and 2017/746 of 5 April 2017, related to medical devices and in vitro medical diagnosis devices, respectively, were published, disrupting years of practices based on European directives. They tend to bring the in vitro diagnosis in Europe c...
Source: Annales de Biologie Clinique - August 30, 2019 Category: Biochemistry Authors: Robinet A, Aljhni R, Fouassier É, Pham P, Bosselut N, Bergouin E, Lemoine A Tags: Ann Biol Clin (Paris) Source Type: research

Medical devices industry: the problem of clinical evaluation reports.
This article examines the background of this requirement while offering medical device manufacturers advice for successful clinical evaluation reports. PMID: 31466939 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 28, 2019 Category: Biochemistry Authors: Egbosimba D Tags: Ann Biol Clin (Paris) Source Type: research

Retours du congr ès EuroMedLab 2019.
Retours du congrès EuroMedLab 2019. Ann Biol Clin (Paris). 2019 Aug 01;77(4):365 Authors: Piéroni L, Sapin V PMID: 31418694 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Piéroni L, Sapin V Tags: Ann Biol Clin (Paris) Source Type: research

Professor Howard Morris, IFCC President (1946-2019).
Authors: PMID: 31418695 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Tags: Ann Biol Clin (Paris) Source Type: research

Biomedical tests outside the nomenclature in France: what are the priorities in nephrology?
PMID: 31418696 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Cristol JP Tags: Ann Biol Clin (Paris) Source Type: research

Measurement of glomerular filtration rate using a reference method.
Abstract Direct measurement methods of glomerular filtration rate (GFR) are considered as the gold standard to assess kidney function. Following the withdrawal of the Proinuline Serb® specialty by the French National Health Surveillance Agency, iohexol remains the most suitable marker to replace inulin as the marker for GFR in France. The assay is performed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection or by mass spectrometry. Plasma clearance measurement is the protocol of choice: single-sample protocols dominate, but multiple-sample protocols may be more accurate in...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Chardon L, Dubourg L, Barin-Le Guellec C, Guinard F, Hannedouche T, Halimi JM, Mariat C Tags: Ann Biol Clin (Paris) Source Type: research

Estimation of glomerular filtration rate using cystatin C.
Abstract Blood concentration of cystatin C is independent of muscle mass and tubular secretion. It can be used, in the absence of a reference method, as an alternative marker to creatinine for the evaluation of renal function and the estimation of glomerular filtration rate (GFR). Both particle-enhanced immunonephelemetry (PENIA) or immunoturbidimetry (PETIA) methods are available to determine cystatin C. From an analytical point of view, it is recommended to use methods whose calibration is traceable to the reference material (ERM-DA471/IFCC) and to report an estimated GFR based on cystatin C. The main equations ...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Bargnoux AS, Barguil Y, Cavalier E, Cristol JP Tags: Ann Biol Clin (Paris) Source Type: research

Urinary exploration of metabolism: nutrition assessment, urolithiasis and tubulopathy.
Abstract The SFBC working group aimed to deal with biological tests outside the french nomenclature that may be useful in the context of urinary exploration of metabolism. This section will be divides into three parts: 1) nutritional assessment using urinary urea; 2) metabolic assessment of urolithiasis; 3) exploration of tubulopathies. National and international recommendations support the evaluation of nutritional status from urea measurements in urine and dialysate with the following indications: primary metabolic evaluation of urolithiasis patients, monitoring of protein intake in chronic renal failure stage 3...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Machon C, Bargnoux AS, Barguil Y, Baron S, Cristol JP, Frochot V Tags: Ann Biol Clin (Paris) Source Type: research

Dialysis monitoring: peritoneal equilibrium test, regional citrate anticoagulation and residual renal function.
Abstract The SFBC working group aimed to deal with biological tests outside the French nomenclature that may be useful for the follow-up of dialysis patients. Our discussion was divided into 3 parts: 1) evaluation of peritoneal membrane characteristics; 2) monitoring of renal replacement therapy using regional citrate anticoagulation; 3) estimation of residual renal function (RRF). International recommendations underline the importance of assessing peritoneal membrane characteristics for peritoneal dialysis prescription. This peritoneal equilibrium test requires the measurement in dialysate of the following parame...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Bargnoux AS, Barguil Y, Zaoui E, Jean G, Cristol JP Tags: Ann Biol Clin (Paris) Source Type: research

Usefulness of free light chain measurement in monoclonal gammopathy, other haematological malignancies and autoimmune diseases.
Abstract Immunoglobulin light chains are called free when they are not linked with heavy chains to form a whole immunoglobulin. Quantification of free light chains is a part of the French national authority for health guidelines for diagnostic and follow-up of light chain, oligo or non-secretory myeloma and AL amyloidosis. Most recently, the World health organisation had included free light chains quantification in prognostic criteria for monoclonal gammopathy of undetermined significance. However the literature bring to light some other potential indications of this analysis in the exploration of monoclonal gammo...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Verebi C, Talbot A, Gendron N, Hurtado-Nedelec M Tags: Ann Biol Clin (Paris) Source Type: research

Insights on glycated albumin.
Abstract Like all proteins, plasma albumin can undergo glycation to produce glycated albumin. This glycation will change the structure and therefore functionalities of albumin. Because of its accessibility, its high concentration and its half-life the glycation of albumin is greater than that of haemoglobin. Laboratory measurement of glycated albumin is possible. The value of glycated albumin reflects the glycaemic balance over a period of 3 weeks, and shows its interest in cases where the determination of HbA1c is impossible or deficient. It also seems that the glycated albumin has a prognostic interest in the ch...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Hay-Lombardie A, Kamel S, Bigot-Corbel E Tags: Ann Biol Clin (Paris) Source Type: research

Can the 72-hour rule based on "Blast/Abn Lymph" flag on Sysmex XN-10 optimize the workflow in hematology laboratory?
In conclusion, the 72-hour rule derived from the GFHC's criteria is secure and reduces the slide review rate and thus the production costs and the turnaround time of hemogram results. Further investigations could confirm that its extension to 144 hours is also adequate. PMID: 31418703 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Paridaens H, Sabor L, Simar J, Ronez E, Cornet E, Defour JP Tags: Ann Biol Clin (Paris) Source Type: research

Interactive pedagogic tools: evaluation of three assessment systems in medical education.
This study provides student perception comparison of ARS. To complete, additional studies are needed to establish their efficacy after several month. PMID: 31418704 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Grzych G, Schraen-Maschke S Tags: Ann Biol Clin (Paris) Source Type: research

Hyperthyroidism: acquired cause of an increase in haemoglobin A2 level.
We report the case of haemoglobin study in a 29-years old man of Mediterranean origin presenting microcytosis. Results showed a slight increase in haemoglobin A2 level, suggestive of a beta-thalassemia trait, which was finally related to the existence of a recently diagnosed Grave's disease. PMID: 31418705 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Stein E, Gérard D, Joly P, Lesesve JF, Perrin J Tags: Ann Biol Clin (Paris) Source Type: research

False paraprotein-induced hypoglycemia in the measurement of glucose by the hexokinase method.
Abstract A 67 years old woman with a Waldenström disease was admitted in the intensive care unit for dyspnea and fever. During hospitalization, episodes of undetectable glycemia were observed without any hypoglycemia symptoms. Plasma glucose was determined with the hexokinase method (recommended). From this observation, a literature review on PubMed was performed to investigate similar cases. In patients with protides in excess (e.g. immunoproliferative syndrome), absorption measurements could be disrupted by the precipitation of excess protein (IgM in most cases). Other parameters could be affected: bilirubi...
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Bossard V, Sauvageon Y, Fraissinet F, Dreyfus B, Thuillier R, Hauet T Tags: Ann Biol Clin (Paris) Source Type: research

Comparison of two kits of anti-infliximab antibodies plasmatic measurement.
CONCLUSION: The assessment of the performance of the tested microplate reader and the validation of the tested ELISA kit showed good results allowing ATI routine measurement to optimize therapeutic management of patients treated by IFX. PMID: 31418707 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Charfi R, Mahmoud I, Ben Salem F, Moalla M, Bouden S, Sfar I, Saïdane O, Klouz A, Daghfous R, Gorgi Y, Abdelmoula L, Trabelsi S Tags: Ann Biol Clin (Paris) Source Type: research

Atazanavir urolithiasis without recent intake of atazanavir.
PMID: 31418708 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Brunel V, Massy N, Malval B Tags: Ann Biol Clin (Paris) Source Type: research

Auer rod-like inclusions in a monoclonal B lymphocytosis.
PMID: 31418709 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Engelman A, Manzoni D, Bordet JC, Vila L Tags: Ann Biol Clin (Paris) Source Type: research

Retour du congr ès EuroMedLab des représentants de la SFBC.
Retour du congrès EuroMedLab des représentants de la SFBC. Ann Biol Clin (Paris). 2019 Aug 01;77(4):465-471 Authors: Baudin B, Boursier G, Delatour V, Gillery P, Gouget B, Gruson D, Piéroni L, Vaubourdolle M PMID: 31418710 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Baudin B, Boursier G, Delatour V, Gillery P, Gouget B, Gruson D, Piéroni L, Vaubourdolle M Tags: Ann Biol Clin (Paris) Source Type: research

Retour du congr ès EuroMedLab par les internes Barcelone, 19-23 mai 2019.
Retour du congrès EuroMedLab par les internes Barcelone, 19-23 mai 2019. Ann Biol Clin (Paris). 2019 Aug 01;77(4):473-478 Authors: Desmous A, Galofaro E, Chtourou M, Grunewald O PMID: 31418711 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - August 1, 2019 Category: Biochemistry Authors: Desmous A, Galofaro E, Chtourou M, Grunewald O Tags: Ann Biol Clin (Paris) Source Type: research