Management of the thrombotic risk associated with COVID-19: what is the role of the hemostasis laboratory?
ier F Abstract COVID-19 is associated with disturbances of hemostasis in the laboratory and an increased thrombotic risk. Routine laboratory tests - activated partial thromboplastin time (aPTT), prothrombin time, Clauss fibrinogen and D-dimers levels measurement - are used for the evaluation of the thrombotic risk and the monitoring of hemostasis, but are subject to several drawbacks that may affect the reliability and clinical relevance of the delivered results. Another challenge for the hemostasis laboratory is the monitoring of heparin treatment. For instance, the issue of the monitoring of unfractionated hepar...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Hardy M, Douxfils J, Dogné JM, Lessire S, Chatelain B, Testa S, Gouin-Thibault I, Gruel Y, Lecompte T, Mullier F Tags: Ann Biol Clin (Paris) Source Type: research

Clinical and biological features of haptoglobin phenotypes.
Abstract Haptoglobin is a late positive acute phase protein of inflammation. Haptoglobin binds to free hemoglobin released from erythrocytes during intravascular hemolysis to form a complex which is removed shortly. Other properties like inhibition of oxidative stress and prostaglandin synthesis have been described. Three main phenotypes of haptoglobin have been identified: Hp1-1, Hp2-1, Hp2-2, which may have an impact in different diseases such as cardiovascular or infectious diseases. Haptoglobins of different phenotypes can be separated by capillary electrophoresis. They may induce a split of the alpha 2-globul...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Simon A, Schneider N, Gillery P, Oudart JB Tags: Ann Biol Clin (Paris) Source Type: research

Management of a global health crisis: first COVID-19 disease feedback from Overseas and French-speaking countries medical biologists.
This study covers all public and private sectors of medical biology located in metropolitan and overseas France and also extends to the French-speaking world. This article presents the testimonies and data obtained for the "Overseas and French-speaking countries" sub-working group made up of 45 volunteer correspondents, located in 20 regions of the world. In view of the delayed spread of the SARS-CoV-2 virus, the overseas regions and the French-speaking regions have benefited from feedback from the first territories confronted with COVID-19. Thus, the entry of the virus or its spread in epidemic form could be avo...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Barguil Y, Chiaradia L, Sicard D, Duhin M, Sebat C, Abdi S, Alomar Y, Blondeel N, Bonnet C, Bouberi-Niava B, Bourgoin-Rousset E, Cavalier E, Cisse F, Combe P, de Guire V, Devaud F, De Wulf A, Agne FD, Dumas-Chastang E, Ecrabey YC, Grignon JC, Gruson D, Gu Tags: Ann Biol Clin (Paris) Source Type: research

Digital morphology analyzers in hematology: a French survey.
CONCLUSION: despite well-established benefits, standardization of practices and technical improvement are still needed. PMID: 33026347 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Grimon N, Ceccomarini T, Maurel-Ribes A, Rieu JB, Mémier V, Dubois-Galopin F Tags: Ann Biol Clin (Paris) Source Type: research

Watch out for a second train.
We report the case of a man with a primary diagnosis of Waldenström macroglobulinemia. He secondarily presented a diffuse large B cell lymphoma (DLBCL) located in the nasal fossae, which relapsed later in the eye. The diagnosis of these two malignancies is based on a multidisciplinary biological approach using new sensitive and specific techniques. These techniques revealed that the two diseases harbor different B cell clones, indicating a distinct origin. This observation highlights the importance of targeted biological techniques for the diagnosis of these two rare hemopathies. It also shows that it is possible to p...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Bravetti C, Le Garff-Tavernier M, De Septenville A, Maloum K, Toutée A, Charlotte F, Lavaud A, Choquet S, Chapiro É, Maillon A, Davi F, Costopoulos M, Degaud M Tags: Ann Biol Clin (Paris) Source Type: research

Recommendations for urinary organic acids analysis.
Abstract Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Organic acid chromatography allows the identification of several hundred compounds and the quantification of the main molecules of interest. As part of the accreditation process for medical biology examinations according to standard NF EN ISO 15189, the group from the French society for inborn errors of metabolism (SFEIM) recommends an approach to accredit organic acid chromatography. Validation parameters and recommendation...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Van Noolen L, Acquaviva-Bourdain C, Dessein AF, Minet-Quinard R, Nowoczyn M, Garnotel R, Corne C Tags: Ann Biol Clin (Paris) Source Type: research

Recommendations for aminoacids chromatography analysis.
Abstract Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Amino acid chromatography allows the identification and quantification of more than forty compounds. As part of the accreditation process for medical biology examinations according to standard NF EN ISO 15189, the group from SFEIM recommends an approach to accredit amino acid chromatography. Validation parameters and recommendations are discussed in this specific framework. PMID: 33026350 [PubMed - as supplied by publis...
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Veauville A, Acquaviva-Bourdain C, Nowoczyn M, Read MH, Corne C, Dessein AF, Van Noolen L, Garnotel R, Minet-Quinard R Tags: Ann Biol Clin (Paris) Source Type: research

Use of « Reference change value » in medical biology: about two examples: total PSA and hemoglobin.
This article describes the methodology used for the RCV calculation, the formatting on analysis reports and the limitations of the system. PMID: 33026351 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Bouverot C, Blachier S, Roquigny N, Guerber F Tags: Ann Biol Clin (Paris) Source Type: research

A curious chronic lymphocytic leukemia.
PMID: 33026352 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - October 1, 2020 Category: Biochemistry Authors: Vergnolle I, Deluche L Tags: Ann Biol Clin (Paris) Source Type: research

Contribution of a molecular test for the diagnosis of genital infection with Trichomonas vaginalis and Mycoplasma genitalium.
Abstract In the present study, we assessed a recently-marketed molecular test, the S-DiaMGTV™ kit (Diagenode), which provides simultaneous detection of Mycoplasma genitalium and Trichomonas vaginalis in urogenital samples. Performance characteristics of the S-DiaMGTV™ kit were compared to an in-house PCR for detection of M. genitalium and, for first time, with direct observation of genital secretions in wet mounting microscopy for T. vaginalis, a routine laboratory method. For M. genitalium, out of 66 samples, two negative with the in-house PCR were found positive with the S-DiaMGTV™ kit and two ...
Source: Annales de Biologie Clinique - September 29, 2020 Category: Biochemistry Authors: Chesnay A, Lancelin B, Le Brun C, Pastuszka A, Desoubeaux G, Lanotte P Tags: Ann Biol Clin (Paris) Source Type: research

Recommendations for acylcarnitine profile analysis.
Abstract Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Acylcarnitine profile allows the identification and quantification of more than thirty compounds. As part of the accreditation process for medical biology examinations according to standard NF EN ISO 15189, the group from SFEIM recommends an approach to accredit acylcarnitine profile. Validation parameters and recommendations are discussed in this specific framework. PMID: 32933890 [PubMed - as supplied by publisher] (S...
Source: Annales de Biologie Clinique - September 14, 2020 Category: Biochemistry Authors: Nowoczyn M, Acquaviva-Bourdain C, Corne C, Minet-Quinard R, Van Noolen L, Garnotel R, Dessein AF Tags: Ann Biol Clin (Paris) Source Type: research

Analysis of the cost effectiveness of different strategies for the antenatal diagnosis of chromosomal aberrations in cases of ultrasound-identified fetal abnormalities.
CONCLUSIONS: CMA appears to be the most effective test for prenatal cytogenetic diagnosis of fetal abnormalities identified by ultrasound. PMID: 32933889 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - September 13, 2020 Category: Biochemistry Authors: Mourgues C, Eymard-Pierre E, Laurichesse-Delmas H, Gerbaud L, Gouas L, Pébrel-Richard C, Vago P, Debost-Legrand A, Goumy C Tags: Ann Biol Clin (Paris) Source Type: research

Biochemical diagnosis of inherited metabolical diseases: metabolic profiles and difficulties for validating methods.
PMID: 32933895 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - September 13, 2020 Category: Biochemistry Authors: Benoist JF, Garnotel R, Acquaviva Bourdain C Tags: Ann Biol Clin (Paris) Source Type: research

Point-of-care testing: place of France in the world National survey in healthcare establisments in 2019.
Abstract The point-of-care tests (POCT) are subject to accreditation. A national inventory survey provides a synthesis of knowledge. The survey distributed 31 questions in 2019. 147 responses were received (75% biologists, 49% CHU, 42% CHG). Only 20.41% are accredited ISO22870, the majority for
Source: Annales de Biologie Clinique - July 31, 2020 Category: Biochemistry Authors: Beauvieux MC, Bost E, Castaing-Mouhica G, Terral C, Berthon N, Martinel I, Chauzit E Tags: Ann Biol Clin (Paris) Source Type: research

An acidosis not so basic.
We present the case of a four-year-old girl, who was hospitalized in intensive care unit for a coma resulting from metabolic acidosis with increased anion gap. The patient was treated for short bowel syndrome, following necrotising enterocolitis, which occurred 51 days after birth. In our initial evaluation of the patient's metabolic acidosis, we were unable to identify the cause of the increased anion gap. Urinary organic acids chromatography identified a large peak of lactate (quantified at 15 mmol/mol of creatiniuria), as well as its metabolites. The discrepancy between normal blood lactate concentration assayed by enzy...
Source: Annales de Biologie Clinique - July 31, 2020 Category: Biochemistry Authors: Lefrère B, Ecochard-Dugelay E, Mosca A, Benoist JF, Hugot JP, Imbard A Tags: Ann Biol Clin (Paris) Source Type: research

Theoretic and practical contribution of bayesian inference to statistical process control: The experience of the Lyon Hospitals Board hemostasis laboratory.
Abstract Laboratories need to set up effective overall management of their internal quality control (IQC) and external quality assessment (EQA) results as key elements in statistical process control. Quality targets need to be defined, with methods to ensure durable control with respect to the relevant specifications. The hemostasis laboratory of the Lyon Hospitals Board (HCL, Lyon, France) uses model 3 from the Milan consensus conference, which is the state of the art in terms of quality targets, and uses a common EQA provider supplying as many real patient samples as possible. Giving priority to adopted methods,...
Source: Annales de Biologie Clinique - July 23, 2020 Category: Biochemistry Authors: Sobas F, Jousselme E, Beghin M, Baillat MG, Perucchetti Y, Nougier C Tags: Ann Biol Clin (Paris) Source Type: research

Analytical and clinical guidelines on neonatal bilirubinemia.
We present in this paper the results of a national harmonization study to progress on this issue. Beyond the analytical aspects, the clinical consequences of harmonization defects were investigated. Finally, guidelines for clinical laboratories are proposed, to be locally adapted. PMID: 32666924 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 14, 2020 Category: Biochemistry Authors: Mailloux A, Cortey A, Delatour V, Poupon C, Rota M, Schmitt F, Vaubourdolle M Tags: Ann Biol Clin (Paris) Source Type: research

A chance diagnosis of Pelger-Hu ët anomaly in a 49-year-old woman hospitalized for an acute episode of Crohn disease.
A chance diagnosis of Pelger-Huët anomaly in a 49-year-old woman hospitalized for an acute episode of Crohn disease. Ann Biol Clin (Paris). 2020 Jul 09;: Authors: Schavgoulidze A, Vergez F, Corre J, Rieu JB PMID: 32644046 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 8, 2020 Category: Biochemistry Authors: Schavgoulidze A, Vergez F, Corre J, Rieu JB Tags: Ann Biol Clin (Paris) Source Type: research

Menkes disease, a diagnosis to consider in case of severe epilepsy  with hyperlactacidemia: a case report.
We report the case of a 3 month-old infant hospitalized due to partial tonic-clonic seizures. Laboratory findings showed increased of lactates in blood and in cerebrospinal fluid. First screenings for infectious, metabolic and genetic causes were negative. After recurrence of multifocal seizures further investigations are made according to the presence of thick and tortuous hair. Low levels of ceruloplasmin and copper in plasma are in agreement with the suspected diagnosis of Menkes disease. Molecular analysis of the ATP7A gene confirmed the diagnosis with a non-sens mutation. PMID: 32633724 [PubMed - as supplied by p...
Source: Annales de Biologie Clinique - July 5, 2020 Category: Biochemistry Authors: Mondesert E, Roubertie A, Girard M, Leboucq N, Baud C, Cuntz D, Cristol JP, Rivier F, Cambonie G, Leydet J, Badiou S Tags: Ann Biol Clin (Paris) Source Type: research

Interactive pedagogical tools could be helpful for medical education continuity during COVID-19 outbreak.
This study showed the importance to have direct contact with a teacher and feedback during a lecture and to not exclusively perform distance learning without direct interaction and feedback. Hence, in the present context, we encourage teacher to use this type of tools to maintain direct interaction with students - which is essential in pedagogy - and ensure a qualitative pedagogical continuity. PMID: 32627731 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 2, 2020 Category: Biochemistry Authors: Grzych G, Schraen-Maschke S Tags: Ann Biol Clin (Paris) Source Type: research

Acute hemolysis crisis revealed a Wilson disease.
We report the diagnostic of Wilson disease in a 14 years old girl and her sibling after investigation of hemolytic anemia, hepatic insufficiency, and hypophosphatemia. PMID: 32618564 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 2, 2020 Category: Biochemistry Authors: Feugray G, Guillerme J, Fraissinet F, Brunel V, Chagraoui A, Forest CD, Lahary A Tags: Ann Biol Clin (Paris) Source Type: research

Water, lithium and sodium: watch out for dangerous injuries.
We report the case of a 64 years old women who presented with global dehydration and severe hypernatremia. Four years ago, she was hospitalized for nephrogenic diabetes insipidus related to a self-induced lithium intoxication. Persistent nephrogenic insipidus diabetes after cessation of lithium therapy are described in literature, and this hypothesis may be consistent with this case report. PMID: 32618565 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 2, 2020 Category: Biochemistry Authors: Fayolle M, Souweine JS, Mathieu O, Bargnoux AS, Cristol JP, Badiou S Tags: Ann Biol Clin (Paris) Source Type: research

Sea-blue histiocytes in the bone marrow of a patient with Niemann-Pick disease type C2.
PMID: 32616471 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 1, 2020 Category: Biochemistry Authors: Fenomanana J, Harzallah I, Martzolff L, Debliquis A Tags: Ann Biol Clin (Paris) Source Type: research

Measurement of angiotensin-I-converting enzyme in the blood: help for method validation.
brahim F, Taibi L Abstract Blood angiotensin-converting enzyme (ACE) assay is now realized by the determination of enzyme activity on synthetic substrate, mostly furylacryloyl-phenylalanyl-L-glycyl-L-glycine (FAPGG). The matrix can be serum or heparin-plasma, with or without a separator; the assay developed on serum or plasma is not adapted to other matrix such as cerebrospinal fluid where the ACE activity is much lower. This assay has been adapted on a number of automated biochemistry analyzers with the specifications of the supplier of reagents, sometimes with modification of volumes or times for analysis. Sampl...
Source: Annales de Biologie Clinique - July 1, 2020 Category: Biochemistry Authors: Baudin B, Zendjabil M, Allouche S, Soudan B, Boutarfa N, Bénéteau-Burnat B, Potestat K, Caussé E, Chaabouni T, Ibrahim F, Taibi L Tags: Ann Biol Clin (Paris) Source Type: research

Echinococcus granulosus  protoscolex in a patient with a pulmonary hydatid cyst.
Echinococcus granulosus protoscolex in a patient with a pulmonary hydatid cyst. Ann Biol Clin (Paris). 2020 Jul 02;: Authors: Aliste-Fernández M, José PS PMID: 32616473 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - July 1, 2020 Category: Biochemistry Authors: Aliste-Fernández M, José PS Tags: Ann Biol Clin (Paris) Source Type: research

Glucose-6-phosphate dehydrogenase deficiency in Tunisian jaundiced neonates.
CONCLUSION: This study shows a higher prevalence of G6PD deficiency in icteric newborns of Tunisia (18.83%). This emphasizes the necessity of neonatal screening for G6PD deficiency to prevent the exposure of these newborns to known hemolytic agents and, subsequently, to prevent kernicterus or other serious complications. PMID: 32576539 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - June 21, 2020 Category: Biochemistry Authors: Dabboubi R, Amri Y, Hamdi S, Jouini H, Sahli C, Fredj SH, Salem KB, ElhoudaToumi N, Messaoud T Tags: Ann Biol Clin (Paris) Source Type: research

Usefulness of immature platelet fraction measurement for diagnosis and monitoring of iron deficiency associated thrombocytopenia: about two cases.
ige L Abstract Iron deficiency anemia is frequently associated with thrombocytosis. However, in some rare cases of very severe iron deficiency, a thrombocytopenia may occur. This condition may lead to a misdiagnosis of immune thrombocytopenic purpura and thus to unnecessary tests in this context. Here we report two patients who presented with iron deficiency associated thrombocytopenia rapidly corrected after martial supplementation. We then discuss the value of measuring immature platelet fraction (IPF), which represents the population of newly formed platelets containing a greater amount of residual RNA. For bot...
Source: Annales de Biologie Clinique - June 21, 2020 Category: Biochemistry Authors: Blandinières A, Arlet JB, Gaussem P, Pouchot J, Darnige L Tags: Ann Biol Clin (Paris) Source Type: research

Transient hyperprolactinemia secondary to benzodiazepines: a case report.
This article aims to increase awareness of prescribing physicians and biologists of the possible occurrence of hyperprolactinemia in patients treated by benzodiazepines, especially since the intake of this molecule is particularly common in France, whether it is a medical prescription or self-medication. PMID: 32576545 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - June 21, 2020 Category: Biochemistry Authors: Blandin L, Siffert M, Brailova M, Sapin V, Maqdasy S, Oris C Tags: Ann Biol Clin (Paris) Source Type: research

Crystalline pathologies in the human body: first steps of pathogenesis.
Abstract The prevalence of crystalline pathologies including urolithiasis, gallstones, vascular calcifications and crystalline arthritis, is very high in the general population beyond 60 years old. Characterization of microcrystals in tissue at the micrometer and at the nanometer scale through physico-chemical techniques constitutes a new opportunity for the physician to decipher the early stage of the pathogenesis of these biological entities. In this review, such description indicates a wide variety of the chemical process associated to the nucleation process directly from supersaturated solution or from organic...
Source: Annales de Biologie Clinique - June 12, 2020 Category: Biochemistry Authors: Bazin D, Letavernier E, Haymann JP, Frochot V, Daudon M Tags: Ann Biol Clin (Paris) Source Type: research

Diagnostic workup in front of an hypereosinophilia in 2020.
Abstract The discovery of eosinophilia above 1.5 G/L should not be considered innocuous, requiring monitoring for etiology and possible secondary organ damage. Among these, cardiac localization is the most worrying, sometimes indolent, to be systematically sought by ultrasound and magnetic resonance. The potential etiologies are very numerous, mostly reactive and corticosensitive, much more rarely clonal in relation to a malignant hemopathy usually chronic and myeloid, sometimes sensitive to tyrosine kinase inhibitors. PMID: 32540797 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - June 12, 2020 Category: Biochemistry Authors: Bonnin A, Vignon G, Mottaz P, Labrousse J, Carrere F, Augereau PF, Aucher P, Lellouche F Tags: Ann Biol Clin (Paris) Source Type: research

Mutltifaceted biological roles of leptin.
Abstract The identification of leptin allowed the discovery of a new endocrine system. This major adipokine controlling energy homeostasis is also involved in the regulation of neuroendocrine function and fertility. Unfortunately, leptin is not able to treat common obesity, which associates hyperleptinemia and resistance to the hormone. Conversely, treatment with recombinant leptin is effective in situations of leptin deficiency. Several pathophysiological situations associated with adipose tissue dysfunctions and abnormal regulation of leptin secretion are discussed in this review. The advantage of the potential ...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Charchour R, Dufour-Rainfray D, Morineau G, Vatier C, Fellahi S, Vigouroux C, Genoux A, Capeau J, Lacorte JM, Collet C, Cuerq C, Bastard JP, groupe de travail RIHN Adipokines Tags: Ann Biol Clin (Paris) Source Type: research

Mutltifaceted biological roles of adiponectin.
kines Abstract Adiponectin is a major adipokine involved in energy homeostasis that exerts insulin-sensitizing properties. The level of adiponectin is reduced in situations of insulin resistance and is negatively associated with several pathophysiological situations including abdominal obesity, metabolic syndrome, steatosis and non-alcoholic steatohepatitis, type 2 diabetes, some cancers and cognitive diseases. These aspects are discussed in this review. PMID: 32540813 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Cuerq C, Morineau G, Dufour-Rainfray D, Vatier C, Fellahi S, Vigouroux C, Genoux A, Lacorte JM, Charchour R, Fève B, Capeau J, Collet C, Bastard JP, groupe de travail RIHN Adipokines Tags: Ann Biol Clin (Paris) Source Type: research

SFBC working group "Biochemical markers of COVID-19".
SFBC working group "Biochemical markers of COVID-19". Ann Biol Clin (Paris). 2020 Jun 01;78(3):269-277 Authors: Beauvieux MC, Bérard AM, Aimone-Gastin I, Barbé F, Barguil Y, Collin-Chavagnac D, Delacour H, Delevallée C, Nivet-Antoine V, Peoc'h K, Poupon C, Schmitt F, Piéroni L, Sapin V, Membres du Groupe de travail SFBC « Marqueurs biochimiques de COVID-19 », Sous-groupe Privés, Sous-groupe Outre-Mer (OM)/francophonie, Sous-groupe CH, Sous-groupe Hôpitaux d’instruction des armées (HIA), Sous-groupe CHU Nord-Est, Sous-groupe CHU Ouest, ...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Beauvieux MC, Bérard AM, Aimone-Gastin I, Barbé F, Barguil Y, Collin-Chavagnac D, Delacour H, Delevallée C, Nivet-Antoine V, Peoc'h K, Poupon C, Schmitt F, Piéroni L, Sapin V, Membres du Groupe de travail SFBC « Marqueurs biochimiques de COVID-19 », Tags: Ann Biol Clin (Paris) Source Type: research

Biomononitoring of environmental exposure to inorganic arsenic.
CONCLUSIONS: The best biomonitoring indicator for iAs exposure is the sum of urine iAs, monmomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) concentrations (SAs). The upper limit of confidence interval of the 95th percentile of the distribution of this parameter in the general adult population living in France is 10 μg/g of creatinine, and is recommended as the limit value for the definition of overexposure. In less than 12 year-old children specific limit values are required, but not yet available. In their absence, SAs should exceed both 10 μg/g creatinine and 11 μg/L to be considered as indicating ...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Garnier R, Goullé JP, Nouyrigat E, Benoit P, Granon C, Manel J, Manouchehri N, Mathieu-Huart A, Nisse P, Normand JC, Ronga-Pezeret S, Roulet A, Simon F, Gabach P, Tournoud C Tags: Ann Biol Clin (Paris) Source Type: research

Update on the diagnosis of parasitic and fungal infections.
zé S Abstract The diagnosis of parasitic and fungal infections, historically based on the detection of these pathogens using direct diagnosis (macro/microscopic examination, culture) or serological methods, has considerably evolved in the last decades, especially with the development of molecular approaches and mass spectrometry. These techniques, as well as most analyses of parasitic and fungal serology, are mostly the preserve of Hospital University Centers Parasitology-Mycology laboratories. In 2016, the French association of medical parasitology and mycology teachers and hospital practitioners (Ano...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Fréalle E, Valot S, Piarroux R, Menotti J, Lachaud L, Persat F, Izri A, Villard O, Yera H, Dannaoui E, Morio F, Houzé S Tags: Ann Biol Clin (Paris) Source Type: research

Association of IVS6A GATT polymorphism of CFTR gene with cystic fibrosis: first study in CF and normal Tunisian population.
Abstract BACKGROUND: Cystic fibrosis (CF) is the most common autosomal recessive disease in Caucasians, caused by mutation in cystic fibrosis transmembrane conductance regulator (CFTR). The analysis of some extra and intragenic markers within or closely linked to CFTR gene is useful as a molecular method in clinical linkage analysis. Indeed, knowing that the molecular basis of CF is highly heterogeneous in our population is explained in the present study. In this work, we are interested for the first time to study the polymorphic marker IVS6a GATT in a CF Tunisian population. METHODS: Our study involved 80 CF...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Chaima S, Sondess HF, Khedija B, Ahmed M, Taieb M Tags: Ann Biol Clin (Paris) Source Type: research

Effect of 99mTc elution in vivo from red cells on red cell volumes measured using autologous 99mTc-labeled red cells: comparison with 51Cr method.
Abstract The purpose of this work was to compare the measured red-cell volume (RCV) using sodium pertechnétate [RCV-99mTc] compared to the reference technique using sodium radiochromate [RCV-51Cr] and to assess the influence of technetium-99 elution on the RCV-99mTc value. Ten patients had simultaneous measurements of RCV-99mTc and RCV-51Cr. Elution of Tc-99m from red blood cells was 2.9% and led to an average overestimation of RCV-99mTc of 3.7%. The introduction of individual tracer elution rates in the RCV-99mTc calculation corrects this overestimation. PMID: 32540818 [PubMed - in process] (Source: A...
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Gallet M, Drouet C, Girodon F, Nicolas A, Riedinger JM Tags: Ann Biol Clin (Paris) Source Type: research

Starvation ketoacidosis during prolonged fasting of 26 days.
Conclusion: We report the case of a patient with fasting ketoacidosis. Besides consequences of this ketoacidosis, the challenge was also in resuming oral feeding in order to avoid a potentially fatal inappropriate renutrition syndrome. PMID: 32540819 [PubMed - in process] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - May 31, 2020 Category: Biochemistry Authors: Lefevre CR, Alix L, Thibault R, Jégo P, Bendavid C, Moreau C Tags: Ann Biol Clin (Paris) Source Type: research

Hereditary pyropo ïkilocytosis diagnosis in an infant: benefit of histograms and peripheral smear review.
Hereditary pyropoïkilocytosis diagnosis in an infant: benefit of histograms and peripheral smear review. Ann Biol Clin (Paris). 2020 May 15;: Authors: Bobée V, Daliphard S, Lahary A PMID: 32420885 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - May 14, 2020 Category: Biochemistry Authors: Bobée V, Daliphard S, Lahary A Tags: Ann Biol Clin (Paris) Source Type: research

Performance criteria for the verification of IgE and tryptase assay methods: recommendations from the AllergoBioNet network.
ogeau S, Tabary T, Uring-Lambert B, Vivinus M, Vitte J Abstract Accreditation of an in vitro diagnostic assay according to the NF/EN/ISO 15189 standard requires to analyze its technical performance before implementation for routine use, and annually when reviewing effectiveness of quality controls. Performance is evaluated through repeatability, intermediate fidelity, accuracy and uncertainty of measurement. The coefficients of variation (CV) of the intra-assay and inter-assay precision tests must be compared with those of "peers" (results from laboratories employing the same method) and also with those ...
Source: Annales de Biologie Clinique - May 14, 2020 Category: Biochemistry Authors: Sarrat A, Couderc R, Alyanakian MA, Apoil PA, Beauvillain C, Chollet L, Chrétien P, Cirée A, Cypriani B, Dumontet E, Evrard B, Garnier L, Grenier A, Guérin V, Hémont C, Léon A, Mariotte D, Nicaise-Roland P, Pernollet M, Rogeau S, Tabary T, Uring-Lamb Tags: Ann Biol Clin (Paris) Source Type: research

Effects of leptin and adiponectin on the cardiovascular system.
Abstract Elevated circulating leptin levels have been associated with an increased cardiovascular risk in humans. However, recent meta-analyses show that certain epidemiological studies did not find this association, suggesting distinct effects of leptin depending on the pathophysiological context. Studies performed in mice deficient in leptin or in leptin receptors are often contradictory, showing both protective and deleterious effects of leptin. These effects appear to vary depending on the genetic background of the animal and the doses of leptin administered, making interpretation of the results difficult. In ...
Source: Annales de Biologie Clinique - May 14, 2020 Category: Biochemistry Authors: Genoux A, Bastard JP Tags: Ann Biol Clin (Paris) Source Type: research

Leptin, adiponectin, lipodystrophic and severe insulin resistance syndromes.
oux C Abstract Leptin and adiponectin are two adipokines currently used as biomarkers for diagnostic orientation and phenotyping in syndromes of lipodystrophy and severe insulin resistance. The level of these biomarkers also has an impact on the therapeutic management of the patients. These aspects, as well as our experience as a reference center, are described in this brief overview. PMID: 32420889 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - May 14, 2020 Category: Biochemistry Authors: Vatier C, Jéru I, Fellahi S, Capeau J, Bastard JP, Vigouroux C Tags: Ann Biol Clin (Paris) Source Type: research

Éditorial.
Éditorial. Ann Biol Clin (Paris). 2020 Apr 01;78(2):125 Authors: Piéroni L PMID: 32319940 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Piéroni L Tags: Ann Biol Clin (Paris) Source Type: research

Sensing of blood glucose by the nervous system: why, where, how?
Abstract Maintaining plasma glucose concentration is a critical requirement for the body, making the detection of blood glucose by the nervous system essential. The reason generally given is that glucose is the main source of energy for living cells. In fact, other reasons make that glucose is so crucial. Glucose feeds two non-oxidative metabolic pathways, glycolysis and the pentose pathway, which allow the synthesis of molecules essential for cell survival and division, such as amino acids or nucleotides. The purpose of this review is to argue this statement and discuss the processes of glucose sensing by the cen...
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Mithieux G Tags: Ann Biol Clin (Paris) Source Type: research

Contamination of urine collected for cytobacteriological examination: situation in a university hospital in Morocco.
CONCLUSION: To reduce urine contamination, quality instructions describing sampling procedures should be available and samples in the middle of the stream and through the collection adhesive bags should be replaced by sus-pubic puncture samples in children, whenever the profit/risk ratio of this method is favourable. PMID: 32319942 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Maleb A, Rifai S, Rahmani N, Bensalah M, Lamrabat S, Benaissa E, Ben Lahlou Y, Frikh M, Elouennass M Tags: Ann Biol Clin (Paris) Source Type: research

Analytical validation of a quantitative method for therapeutic drug monitoring on the Alinity ®c Abbott.
CONCLUSIONS: The analytical validation of a method suitable for therapeutic drug monitoring of drugs on the Alinity®c meets the requirements of European Medicines Agency. PMID: 32319943 [PubMed - as supplied by publisher] (Source: Annales de Biologie Clinique)
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Dubois N, Sqalli G, Gilson M, Charlier C Tags: Ann Biol Clin (Paris) Source Type: research

INR and vitamin K antagonists management by the primary care physicians in Normandy.
Abstract The main objective of our study was to carry out a statement of the knowledge and the management of the VKA by the General Practitioners (GPs) of Normandy and to evaluate their experience of the use of DOA with a questionnaire; 471 of the 1951 GPs requested responded. When the INR was stable in a patient affected with atrial fibrillation, the GPs participating dosed it again 4 weeks later, modified the dosage when the INR was below 1.9 or upper 3.2. The risk of stroke was overestimated to 6.2% per year with fluindione and to 31.5% without curative anticoagulation. The mean TTR was overstated to 84%. When ...
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Druilhe L, Humbert X Tags: Ann Biol Clin (Paris) Source Type: research

Primary breast tuberculosis in a man: a case report.
We report a rare case of primary breast tuberculosis occurring in a male patient. A 33-years-old patient presented with a chronic and fistulized non-inflammatory-right breast swelling with an atrophic cutaneous ulceration. The thoracic CT was in favor of a right breast abscess with a thick wall. The patient had received non-specific antibiotics (amoxicillin-clavulanate and metronidazole) for 10 days coupled with a surgical drainage before consulting us for persisting symptoms. The culture of the pus was sterile, the GeneXpert and the search for acid-fast bacilli (AFB) both performed on the swab of the ulceration were negat...
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Wembulua Shinga B, Ndiaye M, Badiane AS, Belem AR, Andriateloasy S, Aïssatou Lakhe N, Dièye A, Cisse Diallo VMP, Diallo Mbaye K, Ka D, Déguénonvo LF, Seydi M Tags: Ann Biol Clin (Paris) Source Type: research

Value of the syndromic approach for the diagnosis of an imported case of cholera.
lici ML, Pérez B Abstract A 70-year-old woman with no relevant medical history presented banal clinical signs of infectious gastroenteritis on her return from a trip to the Republic of the Union of Myanmar. The appearance of her stools and clinical findings were not suggestive of a typical case of cholera, but Vibrio cholerae was nevertheless isolated from her stools in the laboratory. The National reference center (NRC) for vibrios and cholera identified a Vibrio cholerae serogroup O1 (serotype Inaba) strain. The health authorities were notified of an imported case of cholera, identified on the basis of cl...
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Chalmin S, Lièvre N, Mérillon C, Gallienne E, Leroux V, Léonard PY, Quilici ML, Pérez B Tags: Ann Biol Clin (Paris) Source Type: research

Acute myeloid leukemia with inversion of chromosome 16: cytological, immunophenotypic and cytogenetic disruption.
We report the case of an 18-year-old man with acute myeloid leukemia with unusual inversion of chromosome 16. Cytological, phenotypic and cytogenetic investigations showed a divergence from those in the literature. Indeed, the myelogram shows a medullary infiltration by elements blocked at the stage of myeloblates/promyelocytes, containing Auer rods grouped sometimes in fagots in blasts, promyelocytes and neutrophils. In view of this pathognomonic aspect, the diagnosis of AML type M3 is mentioned but quickly questioned by the results of immunophenotyping in favor of a maturing AML (M2). The karyotype and the FISH later obj...
Source: Annales de Biologie Clinique - March 31, 2020 Category: Biochemistry Authors: Mghinia S, Zaidani M, Hda N, Gamraoui K, Oukkache B Tags: Ann Biol Clin (Paris) Source Type: research