The relevance of ANXA5 genetic variants on male fertility
AbstractPurposeTo investigate the effect of the anticoagulation factor annexin A5 on male fertility and to provide perspective on the influence of members of the coagulation cascade on fertility.MethodsPatients with normozoospermia and with unexplained severe oligozoospermia were retrospectively selected and their genomic DNA sequenced for the promoter region ofANXA5. The genotypes proportions and the odds ratio for carriership of the haplotype M2 were compared between the groups and population control. The clinical data used were gathered from parameters determined during routine clinical assessment and were compared betw...
Source: Journal of Assisted Reproduction and Genetics - June 13, 2019 Category: Reproduction Medicine Source Type: research

Bayesian model for accurate MARSALA (mutated allele revealed by sequencing with aneuploidy and linkage analyses)
ConclusionThe error probability of PGT-M can be significantly reduced by using the Bayesian statistics approach, increasing the accuracy of selecting healthy embryos for transfer with or without a proband sample. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - June 11, 2019 Category: Reproduction Medicine Source Type: research

Cumulus cell pappalysin-1, luteinizing hormone/choriogonadotropin receptor, amphiregulin and hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 mRNA levels associate with oocyte developmental competence and embryo outcomes
ConclusionsCollectively,PAPPA,LHCGR, andAREG mRNA levels in CC may be able to identify oocytes with the best odds of resulting in a live birth, andHSD3B1 mRNA levels may be able to identify oocytes capable of producing euploid embryos. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - June 11, 2019 Category: Reproduction Medicine Source Type: research

Chronically elevated androgen and/or consumption of a Western-style diet impairs oocyte quality and granulosa cell function in the nonhuman primate periovulatory follicle
AbstractPurposeTo investigate the impact of chronically elevated androgens in the presence and absence of an obesogenic diet on oocyte quality in the naturally selected primate periovulatory follicle.MethodsRhesus macaques were treated using a 2-by-2 factorial design (n = 10/treatment) near the onset of menarche with implants containing either cholesterol (C) or testosterone (T, 4–5-fold increase above C) and a standard or “Western-style” diet alone (WSD) or in combination (T+WSD). Following ~ 3.5 years of treatment, females underwent controlled ovula tion (COv,n = ...
Source: Journal of Assisted Reproduction and Genetics - June 11, 2019 Category: Reproduction Medicine Source Type: research

Is younger better? Donor age less than 25 does not predict more favorable outcomes after in vitro fertilization
AbstractObjectiveTo determine whether younger oocyte donor age is associated with better outcomes after in vitro fertilization (IVF) compared with older oocyte donor age.DesignA retrospective cohort study.SettingLarge academically affiliated infertility treatment center.PatientsWe included all women ≥ 18 years who started their first fresh cycle using donor oocytes at our center from January 2002 through October 2017; only the first oocyte recipient cycle was analyzed.InterventionLog-binomial regression was used to compare the incidence of clinical pregnancy and live birth among the following donor age groups:
Source: Journal of Assisted Reproduction and Genetics - June 10, 2019 Category: Reproduction Medicine Source Type: research

Euploid blastocysts implant irrespective of their morphology after NGS-(PGT-A) testing in advanced maternal age patients
ConclusionsAfter eSET, implantation rate was 80 –86%; live birth rate per embryo transfer was 60–73% and clinical miscarriage rate was found to be
Source: Journal of Assisted Reproduction and Genetics - June 4, 2019 Category: Reproduction Medicine Source Type: research

Correction to: Development of an efficient perfusion-based protocol for whole-organ decellularization of the ovine uterus as a human-sized model and in vivo application of the bioscaffolds
The original version of this article unfortunately contained a mistake. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - June 4, 2019 Category: Reproduction Medicine Source Type: research

An update on the aspects of Zika virus infection on male reproductive system
AbstractZika virus (ZIKV) is mainly transmitted through Aedes mosquito bites, but sexual and post-transfusion transmissions have been reported. During acute infection, ZIKV is detectable in most organs and body fluids including human semen. Although it is not currently epidemic, there is a concern that the virus can still reemerge since the male genital tract might harbor persistent reservoirs that could facilitate viral transmission over extended periods, raising concerns among public health and assisted reproductive technologies  (ART) experts and professionals. So far, the consensus is that ZIKV infection in the te...
Source: Journal of Assisted Reproduction and Genetics - May 30, 2019 Category: Reproduction Medicine Source Type: research

The attitude of female students towards sperm donation by their partner
ConclusionsMen rightly worry about their partner or future partner when they donated or consider donating sperm. Only about one in three women would support their partner if he would want to donate. The majority of women perceived sperm donation by their partner as an act that also concerns them and believed that they should be heard in this decision. To promote full informed consent, the relational component should be included in counselling donors, not only regarding the present but also regarding the (possible) future partner. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 30, 2019 Category: Reproduction Medicine Source Type: research

Femtosecond laser is effective tool for zona pellucida engraving and tagging of preimplantation mammalian embryos
ConclusionsOur results demonstrate the suitability of femtosecond laser as a novel tool for noninvasive embryo tagging, enabling embryo identification from day 0.5 post coitum to at least early blastocyst stage. Thus, the versatility and the potential use of femtosecond lasers in the field of developmental biology and assisted reproduction have been shown. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 30, 2019 Category: Reproduction Medicine Source Type: research

The main will of the patients of a private Italian IVF clinic for their aneuploid/affected blastocysts would be donation to research: a currently forbidden choice
ConclusionsDonation of the affected/aneuploid blastocysts is the option chosen from most of the respondents (84%; 15% of the eligible patients). These patients are motivated from the altruistic will of incentivating the progress in IVF and/or stem cell research and supporting future couples to limit/solve their infertility/health issues. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 27, 2019 Category: Reproduction Medicine Source Type: research

Hypertensive disorders of pregnancy and infertility treatment: a population-based survey among United States women
ConclusionsOur results suggest that among U.S. women, the treatment of infertility may be associated with hypertension disorder of pregnancy regardless of type of treatment. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 27, 2019 Category: Reproduction Medicine Source Type: research

The VACTERL association: mosaic mitotic aneuploidy as a cause and a model
AbstractWhile mitotic errors commonly cause aneuploid clones soon after conception, the embryos often normalize as clones are rapidly eliminated. Although generally considered benign, evidence suggests clone elimination as the primary cause of the vertebral, ano-rectal, cardiac, tracheo-esophageal, renal, and limb (VACTERL) association of anomalies, and possibly other adverse outcomes as well. Here, clone elimination-related development disruption at specific locations is used as the basis of a comprehensive theoretical VACTERL association model that also elucidates mitotic mosaic aneuploidy effects. For the association, t...
Source: Journal of Assisted Reproduction and Genetics - May 25, 2019 Category: Reproduction Medicine Source Type: research

Back-to-back random-start ovarian stimulation prior to chemotherapy to maximize oocyte yield
ConclusionsBack-to-back random-start ovarian stimulation may be an effective way to maximize fertility preservation, even in time-limited settings. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 24, 2019 Category: Reproduction Medicine Source Type: research

Epigenetically regulated imprinted gene expression associated with IVF and infertility: possible influence of prenatal stress and depression
ConclusionsIn combination, the studies discussed, as well as two recent projects of our own on placental imprinted gene expression, suggest that lower birthweights in IVF infants are secondary to a combination of exposures including the infertility and prenatal stress that couples undergoing IVF are experiencing. The work highlighted herein emphasizes the need for appropriate control populations that take infertility into account and also for consideration of prenatal psychosocial stressors as confounders and causes of variation in IVF infant outcomes. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 24, 2019 Category: Reproduction Medicine Source Type: research

Cryostorage failures: a medicolegal review
ConclusionProviding cryostorage services is not without significant financial risk. Inadvertent thawing of specimens can lead to high damage awards against cryostorage facilities and those individuals linked to a cryostorage failure event. Because monetary damages can surpass insurance policy limits, those providing cryostorage services should be aware of plaintiff attorney strategies, common legal defenses, and basic asset protection principles to safeguard themselves if ever faced with these situations. Facilities should also carry out regular maintenance and safety checks on equipment and alarm structures to deter such ...
Source: Journal of Assisted Reproduction and Genetics - May 24, 2019 Category: Reproduction Medicine Source Type: research

Interleukin-23 receptor (IL-23R) gene polymorphisms and haplotypes associated with the risk of preeclampsia: evidence from cross-sectional and in silico studies
ConclusionsThe results of present study show positive association between polymorphisms in the IL-23R gene and pre-eclampsia. Therefore, the presence of IL-23R rs11209026, rs10889677 polymorphism might be markers for the genetic susceptibility to pre-eclampsia. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 23, 2019 Category: Reproduction Medicine Source Type: research

Genetic polymorphisms of the cobalamin transport system are associated with idiopathic recurrent implantation failure
ConclusionOur study showed that the polymorphisms ofTCN2 andTCblR are associated with RIF and are potential genetic predisposing factors for RIF among Korean women. Additionally, our findings support a potential role forTCN2 andTCblR in RIF among Korean women. However, further studies are required to investigate the role of the polymorphisms in those proteins and RIF because the roles of theTCN2 andTCblR polymorphisms in RIF are not clear. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 23, 2019 Category: Reproduction Medicine Source Type: research

For whom the egg thaws: insights from an analysis of 10  years of frozen egg thaw data from two UK clinics, 2008–2017
ConclusionsDespite widespread assumptions, many women attempting conception with thawed eggs had not initially frozen them for social reasons. Women who froze their eggs for social reasons presented distinctly and statistically different characteristics at both time of freeze and thaw to women whose eggs were frozen for non-social reasons. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 23, 2019 Category: Reproduction Medicine Source Type: research

A paternal effect of MTHFR SNPs on gametes and embryos should not be overlooked: case reports
(Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 22, 2019 Category: Reproduction Medicine Source Type: research

Do young women with unexplained infertility show manifestations of decreased ovarian reserve?
AbstractPurposeTo investigate whether unexplained infertility at a young age demonstrates manifestations of decreased ovarian reserve.MethodsA total of 100 women were divided into two equally sized groups. The study group comprised women aged ≤ 37 years diagnosed with unexplained infertility, and the control group included age-matched women with either mechanical factor or severe male factor infertility.ResultsBoth groups were comparable in their basic characteristics. Overall, women with unexplained infertility presented with inferior ovarian reserve results set against women of the control group. The numbe...
Source: Journal of Assisted Reproduction and Genetics - May 21, 2019 Category: Reproduction Medicine Source Type: research

Should the flexibility enabled by performing a day-4 embryo transfer remain as a valid option in the IVF laboratory? A systematic review and network meta-analysis
ConclusionsThe aforementioned results may serve as advocates buttressing the option of D4 ET as a valid candidate in the ET decision-making process. Possible limitations of the current study are the publication bias stemming from the retrospective nature of certain included studies, along with various deviations among studies ’ design, referring to number and quality of transferred embryos, or different culture conditions referring to studies of previous decades. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 20, 2019 Category: Reproduction Medicine Source Type: research

Comparison of daily vaginal progesterone gel plus weekly intramuscular progesterone with daily intramuscular progesterone for luteal phase support in single, autologous euploid frozen-thawed embryo transfers
ConclusionsWe did not observe significant differences in the rates of live birth, implantation, and clinical pregnancy between daily IMP and daily VP gel plus weekly IMHPC for LPS in single, autologous euploid FBTs after artificial EP. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 18, 2019 Category: Reproduction Medicine Source Type: research

Maternal serum levels of angiogenic markers and markers of placentation in pregnancies conceived with fresh and vitrified-warmed blastocyst transfer
AbstractPurposeThe aim of the study was to compare the levels of angiogenic markers and markers of placentation between pregnancies conceived with fresh (ET) and vitrified-warmed blastocyst transfer (FET).MethodsWomen with singleton pregnancies resulting from fresh ET or FET during the period between 2013 and 2017 were included in this prospective observational study. Fresh ET was performed in a stimulated and FET in natural cycle. At 6 –7 weeks of gestation, after ultrasound confirmation of a single gestational sac with a viable embryo, serum levels of free β-hCG, pregnancy-associated plasma protein A (PAP...
Source: Journal of Assisted Reproduction and Genetics - May 18, 2019 Category: Reproduction Medicine Source Type: research

Reproductive success of assisted reproductive technology in couples with chromosomal abnormalities
ConclusionAlthough the differences regarding success rate between groups were not found statistically significant, we still advocate that cytogenetic analysis should be performed routinely in all infertile couples namely before ART. This might help deciding the best treatment options including Preimplantation Genetic Testing for aneuploidies or structural rearrangements and minimize the risk of transmission of anomalies to the offspring. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 18, 2019 Category: Reproduction Medicine Source Type: research

Patient-centered elective egg freezing: a binational qualitative study of best practices for women ’s quality of care
ConclusionsSingle women who are pursuing EEF alone in the mostly couples-oriented world of IVF have distinct and multifaceted needs. IVF clinics should strive to make best practices for patient-centered EEF care a high priority. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 18, 2019 Category: Reproduction Medicine Source Type: research

Commentary: Paying a price for plasticity —what the endometrium has to do with it!
(Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 18, 2019 Category: Reproduction Medicine Source Type: research

Correction to: Effect of endometrial mechanical stimulation in an unselected population undergoing in vitro fertilization: futility analysis of a double-blind randomized controlled trial
The original version of this article unfortunately contained mistakes. The complete list of corrections is given below. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 17, 2019 Category: Reproduction Medicine Source Type: research

Development of an efficient perfusion-based protocol for whole-organ decellularization of the ovine uterus as a human-sized model and in vivo application of the bioscaffolds
ConclusionsThis investigation introduces an optimized protocol for whole-organ decellularization of the human-sized uterus with native-like characteristics and a prominent potential for regeneration and angiogenesis which could be employed in in vitro and in vivo studies. To the best of our knowledge, this is the first study to report biomechanical properties and angiographic evaluations of a large animal uterine scaffold. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 16, 2019 Category: Reproduction Medicine Source Type: research

Use of microfluidic sperm extraction chips as an alternative method in patients with recurrent in vitro fertilisation failure
ConclusionsMicrofluidic chip method may be recommended in patients with recurrent unsuccessful in vitro trials. The sperm DNA fragmentation test prior to the treatment will be helpful in selecting the appropriate sperm-washing method. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 15, 2019 Category: Reproduction Medicine Source Type: research

Secretome profile selection of optimal IVF embryos by matrix-assisted laser desorption ionization time-of-flight mass spectrometry
ConclusionA simple, direct and rapid analysis of spent culture fluid from blastocysts at the point of embryo transfer can quickly identify optimal embryos with the best chance of achieving ongoing pregnancy. Methods like this, which take less than 20  min to perform, could dramatically improve the approach to embryo selection and live births. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 15, 2019 Category: Reproduction Medicine Source Type: research

Anti-M üllerian hormone level is associated with vitamin D receptor polymorphisms in women with polycystic ovary syndrome
AbstractObjectiveOur study aimed to investigate the relationship between polymorphisms (Apa1, Bsm1, Fok1, and Cdx2) in the VDR gene as well as AMH and AMHR2 genes and their influence on AMH and 25(OH)D levels in PCOS women.Study designSeventy-five patients with PCOS and 23 control women were included. Serum AMH and 25(OH)D levels in patients and controls were measured by enzyme-linked immunosorbent assay (ELISA). Polymorphisms in VDR gene Fok1 C/T (rs2228587), Bsm1 A/G (rs1544410), Apa1 A/C (rs7975232), and Cdx2 A/G (rs11568820) polymorphisms as well as AMH G/T (rs10407022) and AMHR2 A/G (rs2002555) were analyzed using rea...
Source: Journal of Assisted Reproduction and Genetics - May 14, 2019 Category: Reproduction Medicine Source Type: research

DNA fragmentation in concert with the simultaneous assessment of cell viability in a subfertile population: establishing thresholds of normality both before and after density gradient centrifugation
ConclusionsEstimating total and vital sperm DNA fragmentation, after density gradient centrifugation, increased both the sensitivity and the specificity, thereby lowering the number of false negatives and false positives encountered. These findings provide opportunities to investigate the significance of the total and the vital fractions after different assisted reproductive technologies. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 14, 2019 Category: Reproduction Medicine Source Type: research

Changes in the metabolic composition of storage solution with prolonged cold ischemia of the uterus
The objective of this study was to analyze changes in the metabolic composition of the storage solution in cases of prolonged CI in uteri from ewes.MethodsEighteen uterine auto-transplantations were performed in ewes. CI time was 1  h (T1) or 24 h (T24). Samples of Celsior® were taken when the explanted uterus was flushed (T0) and at the end of CI. A dual approach to metabolic analyses was followed: targeted biochemical analyses targeting several predefined metabolites and non-targeted metabolomics analyses based on nuclear magnetic resonance (NMR).ResultsMetabolic analyses were performed on 16 explanted uter...
Source: Journal of Assisted Reproduction and Genetics - May 12, 2019 Category: Reproduction Medicine Source Type: research

Activation of adenosine monophosphate –activated protein kinase (AMPK) enhances energy metabolism, motility, and fertilizing ability of cryopreserved spermatozoa in domestic cat model
ConclusionsPresence and role of AMPK α protein in cat regulating sperm function were demonstrated before and after cryopreservation. Findings could be used to potentially enhance cryopreserved sperm function in sub-fertile men. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 11, 2019 Category: Reproduction Medicine Source Type: research

PCSK9 and Lp(a) levels of children born after assisted reproduction technologies
AbstractPurposeProprotein convertase subtilisin/kexin type 9 (PCSK9) and lipoprotein (a) (Lp[a]) levels are associated with cardiovascular risk. To investigate PCSK9 and Lp(a) levels of children born after assisted reproduction technologies (ART) compared with naturally conceived (NC) controls.MethodsIn this exposure-matched cohort study, 73 racial-, sex-, and age-matched children (mean age 98  ± 35 months) of ART (intracytoplasmic sperm injection [ICSI]n = 33, classic in vitro fertilization [IVF]n = 40) and 73 NC children were assessed. Blood lipid profile, including PCSK9...
Source: Journal of Assisted Reproduction and Genetics - May 11, 2019 Category: Reproduction Medicine Source Type: research

Molecular mechanism of FSHR expression induced by BMP15 in human granulosa cells
ConclusionBMP15 induced FSHR expression in human granulosa cells through Smad and non-Smad pathways. This mechanism of FSHR induction by BMP15 may be utilized for controlling follicular growth. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 11, 2019 Category: Reproduction Medicine Source Type: research

Patient-centered communication, patient satisfaction, and retention in care in assisted reproductive technology visits
ConclusionsContrary to what was expected, the style of physician-patient communication was not found to be associated with patient satisfaction and retention in care. However, patients were highly satisfied and engaged. The actual meaning of a communication that is “patient-centered” in the ART context might be wider, including the couples’ need for information, as suggested by qualitative findings. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 10, 2019 Category: Reproduction Medicine Source Type: research

Decreased very long chain polyunsaturated fatty acids in sperm correlates with sperm quantity and quality
ConclusionThis study revealed a positive correlation between the levels of VLC-PUFA with sperm count and total motile count and suggests that both sperm quality and quantity may depend on the presence of VLC-PUFA. The lack of correlation between VLC-PUFA and DHA suggests that low VLC-PUFA levels do not result from inadequate PUFA precursors. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Menstrual cycle-dependent alterations in glycosylation: a roadmap for defining biomarkers of favorable and unfavorable mucus
Conclusion(s)We identified molecular signatures of unfavorable mucus from women in the luteal phase and on hormonal contraceptives. Further characterization of these biomarkers may be useful in contraceptive development and in evaluation of infertility. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Nonstop mutation in the Kisspeptin 1 receptor (KISS1R) gene causes normosmic congenital hypogonadotropic hypogonadism
ConclusionsOur study highlights the importance of the KISS1R signaling, in gonadotropin-releasing hormone neurons, in the control of reproductive function. Additionally, our data suggests a complex central and peripheral metabolic control of puberty, through the hypothalamic KISS1R signaling. We suggest a mutual link between the hypothalamic –pituitary–gonadal, –adrenal, and –somatotropic axes. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer
AbstractPurposeTo assess meiotic and developmental competence after transfer of immature cumulus-oocyte complexes (COCs) to the preovulatory follicles of mares (intrafollicular oocyte transfer (IFOT)).MethodsIn Experiment 1, mares received an ovulatory stimulus at IFOT. Thirty hours later, COCs were recovered from the follicle, and mature oocytes underwent ICSI and embryo culture. In Experiments 2 and 3, autologous vs. allogeneic COCs were used. The mares were inseminated and embryos were recovered. In Experiment 3, the ovulatory stimulus was administered 9  h (autologous) and 15 h (allogeneic) before IFOT. In Ex...
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Down syndrome and infertility: what support should we provide?
AbstractDown syndrome (DS) is the most common genetic disease at birth; on average, it affects 1 in 700 newborns. The syndrome features cognitive impairment, susceptibility to certain diseases, and (in some cases) congenital malformations. Improvements in medical care for people with DS have led to an increase in life expectancy. Furthermore, the systematic provision of specific support during childhood improves cognitive function and autonomy in adulthood. Consequently, patients and their families are now seeking the same rights as healthy people. Access to procreation is an emerging debate. The presumption of infertility...
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Serum and follicular fluid fetuin-B levels are correlated with fertilization rates in conventional IVF cycles
AbstractPurposeTo investigate the relationship between serum/follicular fluid fetuin-B levels and fertilization outcomes in conventional IVF cycles.MethodsA prospective cohort study of conventional IVF treatments including 78 cycles with low fertilization rates (two pronuclei [2PN] rate    70% (high fertilization group, HF). To calculate the required sample size, a two-samplet test power analysis was applied to data from our pilot study, using PASS 11.0 software. Fetuin-B was measured using a commercial sandwich enzyme-linked immunosorbent assay.ResultsSerum fetuin-B and follicular fluid fetuin-B were positiv...
Source: Journal of Assisted Reproduction and Genetics - May 9, 2019 Category: Reproduction Medicine Source Type: research

Can follicular Emmprin and BMP 4 levels predict ICSI outcome?
ConclusionClinical pregnancy rates after ICSI may be associated with follicular fluid levels of Emmprin and BMP 4. Follicular levels of Emmprin and BMP 4 can be used as a marker (as markers for predicting ICSI outcomes) for a better ICSI outcome. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - May 8, 2019 Category: Reproduction Medicine Source Type: research

Maternal body mass index affects embryo morphokinetics: a time-lapse study
AbstractPurposeTo assess the effect of body mass index (BMI) on morphokinetic parameters of human embryos evaluated with time-lapse technology during in vitro culture.MethodsA retrospective analysis of ART cycles utilizing time-lapse technology was undertaken to assess the potential impact of maternal BMI on morphokinetic and static morphological parameters of embryo development. The cohort of patients was divided into four groups: 593 embryos from 128 underweight women in group A; 5248 embryos from 1107 normal weight women in group B; 1053 embryos from 226 overweight women in group C; and 286 embryos from 67 obese women i...
Source: Journal of Assisted Reproduction and Genetics - May 6, 2019 Category: Reproduction Medicine Source Type: research

Compromised global embryonic transcriptome associated with advanced maternal age
AbstractPurposeTo investigate the global transcriptome and associated embryonic molecular networks impacted with advanced maternal age (AMA).MethodsBlastocysts derived from donor oocyte IVF cycles with no male factor infertility (
Source: Journal of Assisted Reproduction and Genetics - April 25, 2019 Category: Reproduction Medicine Source Type: research

Meddling with new technologies or amending an embryos ’ potential
(Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - April 24, 2019 Category: Reproduction Medicine Source Type: research

Understanding mechanisms of oocyte development by follicular fluid lipidomics
ConclusionHuman FF fingerprinting can both improve the understanding concerning mechanisms responsible for oocyte development and its effect on embryo implantation potential and assist in the management of IVF cycles. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - April 23, 2019 Category: Reproduction Medicine Source Type: research

Gene expression analysis of follicular cells revealed inflammation as a potential IVF failure cause
ConclusionsOur results suggest that failure to conceive following IVF cycles could be associated with an imbalance between pro-inflammatory and anti-inflammatory mediators. The findings of this study identify potential failure causes and pathways for further investigation. Stimulatory protocols personalized according to patient response could improve the chances of later success. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - April 18, 2019 Category: Reproduction Medicine Source Type: research