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Mining phase separation-related diagnostic biomarkers for endometriosis through WGCNA and multiple machine learning techniques: a retrospective and nomogram study
ConclusionsThis study suggests the potential involvement of phase separation-related genes in the pathogenesis of endometriosis (EMs) and identifies promising biomarkers for diagnosis. These findings have implications for further research and the development of new therapeutic strategies for EMs. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 8, 2024 Category: Reproduction Medicine Source Type: research

Correction to: Optimizing non ‑invasive preimplantation genetic testing: investigating culture conditions, sample collection, and IVF treatment for improved non‑invasive PGT‑A results
(Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 7, 2024 Category: Reproduction Medicine Source Type: research

High resolution dynamic ultrasound atlas of embryonic and fetal development of the common marmoset
ConclusionThe temporal and spatial resolution of ultrasound achieved in this study demonstrates the promise of noninvasive imaging in the marmoset for the in vivo study of primate-specific aspects of embryonic and fetal development. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 6, 2024 Category: Reproduction Medicine Source Type: research

Histological analysis of (antral) follicle density in ovarian cortex tissue attached to stripped endometriomas
ConclusionsOvarian tissue attached to stripped endometriomas holds potential as a non-invasive source for antral follicles. In theory, application of IVM could be an interesting alternative FP option in young patients with endometriomas who undergo cystectomy in order to transform the surgical collateral damage to a potential oocyte source. Our results encourage future research with fresh tissue to further assess the quality and potential of these follicles.Trial registrationClinical Trials.gov Identifier: B21.055 (METC LDD), date of registration 12 –08-2021, retrospectively registered. (Source: Journal of Assisted Repro...
Source: Journal of Assisted Reproduction and Genetics - March 5, 2024 Category: Reproduction Medicine Source Type: research

Seminal cell –free DNA as a potential marker for in vitro fertility of Nellore bulls
ConclusionThe concentration of cfDNA and the relative number of copies of cfmtDNA in seminal plasma cannot predict the freezability of semen but can be used to predict in vitro embryo production. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 5, 2024 Category: Reproduction Medicine Source Type: research

Influx of zwitterionic buffer after intracytoplasmic sperm injection (ICSI) membrane piercing alters the transcriptome of human oocytes
ConclusionThe influence of zwitterionic buffer on intrinsic cellular mechanisms provides numerous concerns for their use in IVF clinical applications. The primary concern is the ICSI procedure, in which the surrounding buffer is allowed influx into the oocytes after membrane piercing. Selecting a physiological bicarbonate buffer may reduce imposed stress on oocytes, resulting in improved embryo development and clinical results because intracellular MOPS, and especially HEPES, may negatively impact intrinsic biological mechanisms, as revealed by transcriptome changes. These find ings further support the utilization of bic...
Source: Journal of Assisted Reproduction and Genetics - March 4, 2024 Category: Reproduction Medicine Source Type: research

Ethical, legal, social, and policy issues of ovarian tissue cryopreservation in prepubertal girls: a critical interpretive review
ConclusionThis first literature review focusing on ethical, legal, social, and policy issues surrounding OTC in prepubertal girls highlights concerns in the oncofertility debate. Although OTC is no longer experimental as of December 2019, these issues could limit its availability and the child ’s future reproductive autonomy. This review concludes that specific actions must be provided to enable the offer of FP, such as supporting families’ decision-making in this unique and complex context, and providing pediatric patients universal and full access to free or highly subsidized OTC. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 2, 2024 Category: Reproduction Medicine Source Type: research

Achieving an optimal pregnancy outcome through the combined utilization of micro-TESE and ICSI in cryptorchidism associated with a non-canonical splicing variant in RXFP2
ConclusionThis is the first cryptorchidism case carrying a novel causative non-canonical splicingRXFP2 variant. The combined approach of micro-TESE and ICSI contributed to an optimal pregnancy outcome. Our literature review demonstrated thatRXFP2 variants caused cryptorchidism in a recessive inheritance pattern, rather than a dominant pattern. (Source: Journal of Assisted Reproduction and Genetics)
Source: Journal of Assisted Reproduction and Genetics - March 2, 2024 Category: Reproduction Medicine Source Type: research