Characterization and expression analysis of seven putative JHBPs in the mud crab Scylla paramamosain: Putative relationship with methyl farnesoate
In this study, seven JHBPs from mud crab Scylla paramamosain were obtained. All Sp-JHBPs contained one JHBP domain, and Sp-JHBP4, 5, 6 also contained one Grp7_allergen domain. The predicted secondary and 3D structures are conserved among the seven Sp-JHBP domains, but the complete 3D structure of Sp-JHBP4, 5, 6 were more similar to lipopolysaccharide binding protein. Additionally, the potential ligands for Sp-JHBP1, 2, 3, 7 were ubiquinone 8 (UQ8) and JH II or JH III, and the predicted binding location and sites for JHs were similar to the structured known BmJHBP, suggesting that MF is a potential ligand for these Sp-JHBPs...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - December 7, 2019 Category: Molecular Biology Source Type: research

A review on fish immuno-nutritional response to indispensable amino acids in relation to TOR, NF-κB and Nrf2 signaling pathways: Trends and prospects
Publication date: Available online 5 December 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Habte-Michael Habte-TsionAbstractIndispensable amino acids (IAAs) are important regulators of key metabolic pathways associated with protein synthesis, tight junction proteins, inflammatory cytokines and immune-antioxidant related signaling molecules. However, the information pertaining to the immune functions of IAAs in relation to molecular approaches for commercially important fish species are scarce and discordant. This review summarizes the dietary requirements for IAAs...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - December 7, 2019 Category: Molecular Biology Source Type: research

Enzymatic activities in the digestive tract of spirostreptid and spirobolid millipedes (Diplopoda: Spirostreptida and Spirobolida)
Publication date: Available online 18 November 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): V. Šustr, S. Semanová, M.M. Rost-Roszkowska, K. Tajovský, A. Sosinka, F. KaszubaAbstractMillipedes represent a model for the study of organic matter transformation, animal-microbial interactions, and compartmentalisation of digestion. The activity of saccharidases (amylase, laminarinase, cellulase, xylanase, chitinase, maltase, cellobiase, and trehalase) and protease were measured in the midgut and hindgut contents and walls of the millipedes Archispiro...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 19, 2019 Category: Molecular Biology Source Type: research

PmCBP, a novel poly (chitin-binding domain) gene, participates in nacreous layer formation of Pinctada fucata martensii
In this study, a novel chitin-binding protein from Pinctada fucata martensii (PmCBP) with poly (chitin-binding domain) was cloned, which contains a 5′-untranslated region (UTR) of 114 bp and 3′UTR of 116 bp, and encodes a putative protein of 2044 amino acids. The predicted PmCBP protein was structurally typical of the CBP family with 20 ChtBD2 domains. Phylogenetic and linear relation analyses showed that the ChtBD2 domain has a highly conserved structure among the three species of P. f. martensii, Crassostrea gigas, and Mizuhopecten yessoensis. qRT-PCR and in-situ hybridization analysis revealed that PmCBP...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 15, 2019 Category: Molecular Biology Source Type: research

Circadian rhythm regulation of the oxidation–antioxidant balance in Daphnia pulex
Publication date: Available online 14 November 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Mingqi Cai, Zhiquan Liu, Ping Yu, Yang Jiao, Qiang Chen, Qichen Jiang, Yunlong ZhaoAbstractRhythmic changes in reactive oxygen species (ROS) levels with time have been previously reported, but circadian rhythmic changes in ROS levels in Daphnia pulex remain unexplored. Here, in light–dark (L/D 16:8, L/D 8:16), continuous light (LL), and continuous dark (DD) conditions were set up to analyze the accumulation of ROS and activity of antioxidant enzymes in D. pulex at dif...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 15, 2019 Category: Molecular Biology Source Type: research

Molecular characterization and expression of two interleukin-17 receptor A genes on different chromosomes in Japanese medaka, Oryzias latipes
In this study, the nucleotide sequence of IL-17RA1 cDNA from Japanese medaka (Oryzias latipes) of the Cab strain was determined and compared to two IL-17RA cDNAs (i.e., IL-17RA1 and IL-17RA2) of Japanese medaka Hd-rR strain downloaded from NCBI. Hd-rR 17RA1 and IL-17RA2 were located on chromosome 23 and chromosome 6, respectively, and phylogenetic tree analysis revealed that teleost IL-17RA1 and IL-17RA2 were separated in different clusters. Synteny analysis revealed that Japanese medaka IL-17RA1 and mammalian IL-17RA are conserved. IL-17RA1 expression levels in the gills, intestine, whole kidney, skin, and spleen were sig...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 12, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: January 2020Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 239Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 9, 2019 Category: Molecular Biology Source Type: research

In Appreciation
Publication date: January 2020Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 239Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 9, 2019 Category: Molecular Biology Source Type: research

Cloning and characterization of a novel Lustrin A gene from Haliotis discus hannai
In this study, we isolated and cloned the complete cDNA of Lustrin A from Haliotis discus hannai, which was named Hdh-Lustrin A. Hdh-Lustrin A has characteristic cysteine- and proline-rich domains, glycine- and serine-rich domains, and a whey acidic protein (WAP)-like C-terminus. The cysteine- and proline-rich domains showed internal similarity repeats that arrayed in gene coding region, and the phylogenetic tree of these repeats indicated that the similarity of structural repetitive unit components in different abalone species, reflecting their evolutionary distance. A tissue distribution analysis showed that the mRNA lev...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 8, 2019 Category: Molecular Biology Source Type: research

Sterol-O acyltransferase 1 is inhibited by gga-miR-181a-5p and gga-miR-429-3p through the TGFβ pathway in endodermal epithelial cells of Japanese quail
Publication date: Available online 31 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Han-Jen Lin, Chiao-Wei Lin, Harry J. Mersmann, Shih-Torng DingAbstractNutrients are utilized and re-constructed by endodermal epithelial cells (EECs) of yolk sac membrane (YSM) in avian species during embryonic development. Sterol O-acyltransferase 1 (SOAT1) is the key enzyme to convert cholesterol to cholesteryl ester for delivery to growing embryos. During embryonic development, yolk absorption is concomitant with significant changes of SOAT1 mRNA concentration and enzyme ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 1, 2019 Category: Molecular Biology Source Type: research

Heart mitochondria from naked mole-rats (Heterocephalus glaber) are more coupled, but similarly susceptible to anoxia-reoxygenation stress than in laboratory mice (Mus musculus)
Publication date: Available online 31 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): G.Y. Lau, W.K. Milsom, J.G. Richards, M.E. PamenterAbstractNaked mole-rats (Heterocephalus glaber; NMRs) are among the most hypoxia-tolerant mammals described to date and exhibit plastic responses during hypoxia exposure. The goal of the present study was to determine if heart mitochondria from NMRs functionally differ from those of hypoxia-intolerant common laboratory mice (Mus musculus). We assessed heart mitochondrial respiratory flux, proton leak kinetics, responses to i...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 1, 2019 Category: Molecular Biology Source Type: research

The catadromous teleost Anguilla japonica has a complete enzymatic repertoire for the biosynthesis of docosahexaenoic acid from α-linolenic acid: Cloning and functional characterization of an Elovl2 elongase
Publication date: Available online 31 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Wenju Xu, Shuqi Wang, Cuihong You, Yueling Zhang, Óscar Monroig, Douglas R. Tocher, Yuanyou LiAbstractThe Japanese eel Anguilla japonica is a catadromous fish species with considerable farming scale. Previous studies showed that dietary α-linolenic acid (18:3n-3) and linoleic acid (18:2n-6) satisfied essential fatty acid requirements in eel, which suggested that Japanese eel should have a complete pathway for the biosynthesis of long-chain polyunsaturated fatty ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - November 1, 2019 Category: Molecular Biology Source Type: research

Molecular characterization, expression analysis of myostatin gene and its negative regulation by miR-29b-3p in Chinese concave-eared frogs (Odorrana tormota)
Publication date: Available online 30 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Yilin Shu, Dong Tang, Shuakat Ali Khan, Jun He, Huijuan Zhang, Li Sun, Hailong Wu, Linming LuAbstractThe molecular characteristics, expression patterns and functions of the amphibian myostatin (MSTN) gene are unknown. Here, we isolated a full-length Odorrana tormota MSTN cDNA sequence of 1701 bp (Ot-MSTN), containing a putative N-terminal signal peptide, a TGF-β propeptide domain and an active peptide. Ot-MSTN was expressed in 9 selected tissues examined, and the high...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 31, 2019 Category: Molecular Biology Source Type: research

Effects of feeding and digestion on myocardial contractility and expression of calcium-handling proteins in Burmese pythons (Python molurus)
Publication date: Available online 30 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Eliton da Silva Vasconcelos, Ana Lúcia Kalinin, Rafael Correa Cipriano, Samuel dos Santos Beserra, André Guelli Lopes, Cléo Alcântara da Costa Leite, Diana Amaral MonteiroAbstractPythons are important models of studies on postprandial metabolism because their physiological responses are exacerbated when digesting large prey. Prior studies of these animals have shown hypertrophy of the cardiac tissue 2 to 3 days after feeding, coinciding with the ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 31, 2019 Category: Molecular Biology Source Type: research

Review: The structure and function of cellulase (endo-β-1,4-glucanase) and hemicellulase (β-1,3-glucanase and endo-β-1,4-mannase) enzymes in invertebrates that consume materials ranging from microbes, algae to leaf litter
Publication date: February 2020Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 240Author(s): Stuart M. LintonAbstractThis review discusses the reaction catalysed, and the structure and function of the cellulase, endo-β-1,4-glucanase and the hemicellulase enzymes, β-1,3-glucanase and endo-β-1,4-mannase that are present in numerous invertebrate groups with a diverse range of feeding specialisations. These range from microbial deposit and filter feeders, micro and macrophagous algal feeders, omnivores to herbivorous leaf litter and wood feeders. Endo-β-1,4...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 26, 2019 Category: Molecular Biology Source Type: research

Generation of MuRF-GFP transgenic zebrafish models for investigating murf gene expression and protein localization in Smyd1b and Hsp90α1 knockdown embryos
Publication date: Available online 24 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Baojun Li, Siping Li, Qiuxia He, Shaojun DuAbstractMuscle-specific RING-finger proteins (MuRFs) are E3 ubiquitin ligases that play important roles in protein quality control in skeletal and cardiac muscles. Here we characterized murf gene expression and protein localization in zebrafish embryos. We found that the zebrafish genome contains six murf genes, including murf1a, murf1b, murf2a, murf2b, murf3 and a murf2-like gene that are specifically expressed in skeletal and card...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 26, 2019 Category: Molecular Biology Source Type: research

Molecular and functional characterisation of a putative elovl4 gene and its expression in response to dietary fatty acid profile in Atlantic bluefin tuna (Thunnus thynnus)
Publication date: Available online 24 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Mónica B. Betancor, Angela Oboh, Aurelio Ortega, Gabriel Mourente, Juan C. Navarro, Fernando de la Gándara, Douglas R. Tocher, Óscar MonroigAbstractElongation of very long-chain fatty acid 4 (Elovl4) proteins are involved in the biosynthesis of very long-chain (>C24) fatty acids and in many teleost fish species they are key enzymes in the pathway for the production of docosahexaenoic acid (DHA; 22:6n-3) from eicosapentaenoic acid (EPA; 20:5n-3). There...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 26, 2019 Category: Molecular Biology Source Type: research

Molecular characterization of a novel sulfide:quinone oxidoreductase from the razor clam Sinonovacula constricta and its expression response to sulfide stress
Publication date: Available online 24 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Yaoyao Shen, Jiaqi Chen, Weiliang Shen, Caifang Chen, Zhihua Lin, Chenghua LiAbstractThe razor clam Sinonovacula constricta is a commercial benthic bivalve, and burrows the deeper cave than the other buried benthic bivalves. Due to the little exchange of seawater and to anoxic conditions, S. constricta is exposed to considerable amounts of sulfide during low tide, but exhibits strong sulfide tolerance. Mitochondrial sulfide oxidation is a particular defense strategy against ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 26, 2019 Category: Molecular Biology Source Type: research

Expression of specific corneous beta proteins in the developing digits of the Japanese gecko (Gekko japonicus) reveals their role in the growth of adhesive setae
Publication date: Available online 24 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Feifei Wang, Mingyue Chen, Fengna Cai, Peng Li, Jie Yan, Kaiya ZhouAbstractGeckos possess strong adhesion ability, even can climb on smooth surface. Previous studies have shown that the setae of geckos play a crucial role in their ability to climb on vertical walls. But the biological molecular mechanism of their adhesion ability remains unclear. In the present study, the expression patterns of corneous beta proteins (CBPs) genes related to claws, scales, and feathers develo...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 26, 2019 Category: Molecular Biology Source Type: research

Invited review: The structure and function of cellulase (endo-β-1,4-glucanase) and hemicellulase (β-1,3-glucanase and endo-β-1,4-mannase) enzymes in invertebrates that consume materials ranging from microbes, algae to leaf litter
Publication date: Available online 21 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Stuart M. LintonAbstractThis review discusses the reaction catalysed, and the structure and function of the cellulase, endo-β-1,4-glucanase and the hemicellulase enzymes, β-1,3-glucanase and endo-β-1,4-mannase that are present in numerous invertebrate groups with a diverse range of feeding specialisations. These range from microbial deposit and filter feeders, micro and macrophagous algal feeders, omnivores to herbivorous leaf litter and wood feeders. Endo-&be...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 22, 2019 Category: Molecular Biology Source Type: research

Thymosin-β12 characteristics and function in Urechis unicinctus
In this study, we cloned the Tβ12 gene with an ORF of 126 bp coding 41 amino acids from Urechis unicinctus. Tissue distribution analysis by qRT-PCR used TBP as reference gene showed that Tβ12 was widely expressed in all tissues, and the transcript levels were the highest in the body wall, followed by the coelomic fluid, and the lowest in the intestines and anal sacs. After LPS (lipopolysaccharides) injection, Tβ12 expression in the body was first elevated significantly at 3 h (p 
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 22, 2019 Category: Molecular Biology Source Type: research

Comparative analysis of β-hexosaminidase and acid phosphatase from Hydra vulgaris Ind-Pune, H. vulgaris Naukuchiatal and H. magnipapillata sf-1: Localization studies of acid phosphatase and β-hexosaminidase from H. vulgaris Ind-Pune
Publication date: Available online 17 October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Rohit Sai Reddy Konada, Lakshmi Surekha Krishnapati, Ashapogu Venugopal, Chung-Hung Lin, Siva Kumar NadimpalliAbstractThe present report describes a comprehensive study on comparative biochemical characterization of two lysosomal enzymes, acid phosphatase and β-hexosaminidase in three different strains of Hydra; Hydra vulgaris Ind-Pune, H. vulgaris Naukuchiatal and H. magnipapillata sf-1 (self-feeder-1). Since morphology and habitat of Hydra effect lysosomal enzymes and...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 19, 2019 Category: Molecular Biology Source Type: research

Characterization of PHB in the gonadal development of the swimming crab Portunus trituberculatus
In this study, we cloned the PHB gene from the testis of the swimming crab Portunus trituberculatus (PtPHB) and analyzed the deduced amino acid sequence. The expression level of phb mRNA in larvae was analyzed using qRT-PCR. The expression level of phb mRNA and PHB protein in different tissues were analyzed using qRT-PCR and Western blot respectively. Enzyme-linked immunosorbent assay analyses of the PHB protein were conducted with the testis and ovaries from P. trituberculatus specimens at different developmental stages. PHB was localized with mitochondria and ubiquitin in the testis and ovaries. The PtPHB gene was found ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 19, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: December 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 238Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - October 13, 2019 Category: Molecular Biology Source Type: research

Identification of duplicated suppressor of cytokine signaling 3 (SOCS3) genes in blunt snout bream (Megalobrama amblycephala)
In this study, we identified socs3s genes of blunt snout bream. 209- and 216-aa long peptides are encoded by socs3a and socs3b genes, respectively. The socs3s mRNAs are expressed consistently during the entire process of embryonic development. Whole-mount in situ hybridization detected socs3a in the eyes and posterior somites at 12 h post fertilization (hpf), transcribed at the otic vesicle at 24 hpf, and transcribed at the eyes, brain, and otic vesicle at 36 hpf; while the socs3b mRNA was transcribed at the notochord at 12 hpf, expressed in the brain, eyes, and tailbud at 24 hpf, and detected in the brain at 36 hpf....
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 15, 2019 Category: Molecular Biology Source Type: research

Comparative proteomic analysis of senescence in the freshwater cladoceran Daphnia pulex
In this study, we used LC-MS/MS with eight-plex iTRAQ to perform a comparative proteomic analysis of senescence in Daphnia pulex. Of 3076 primordial proteins, 2325 were credible (the remaining were low-confidence proteins) and 247 significantly differentially expressed proteins (DEPs). Of the latter, 87, 91, and 69 DEPs were identified in the Day 15 vs. Day 5, Day 20 vs. Day 5, and Day 25 vs. Day 5 groups, respectively. Gene ontology enrichment analysis showed that oxidative damage may be the main cause of senescence in D. pulex. Using Kyoto Encyclopedia of Genes and Genomes pathway analysis, we found that the peroxisome p...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 15, 2019 Category: Molecular Biology Source Type: research

Hepatocyte nuclear factor 4α (Hnf4α) is involved in transcriptional regulation of Δ6/Δ5 fatty acyl desaturase (fad) gene expression in marine teleost Siganus canaliculatus
Publication date: Available online 13 September 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Yewei Dong, Shuqi Wang, Cuihong You, Dizhi Xie, Qingyan Jiang, Yuanyou LiAbstractAs the first marine teleost demonstrated to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs) from C18 precursors such as linoleic acid (LOA, 18:2n-6) and α-linolenic acid (ALA, 18:3n-3), the rabbitfish (Siganus canaliculatus) contains the complete enzymatic system for LC-PUFA biosynthesis, including Δ6/Δ5 fatty acid desaturase (Fad), Δ4 Fad, and elongase ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 15, 2019 Category: Molecular Biology Source Type: research

Expression of glut2 in response to glucose load, insulin and glucagon in grass carp (Ctenophcuyngodon idellus)
Publication date: Available online 10 September 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Wenli Zhao, Chaobin Qin, Guokun Yang, Xiao Yan, Xiaolin Meng, Liping Yang, Ronghua Lu, Dapeng Deng, Mingming Niu, Guoxing NieAbstractGenerally, fish are thought to have a limited ability to utilize carbohydrate. Postprandial blood glucose is cleared sluggishly in fish, resulting in prolonged hyperglycemia. Facilitative glucose transporters (GLUTs) play an important role in glucose utilization. In the present study, the expression levels of glut2 in different tissues were d...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 12, 2019 Category: Molecular Biology Source Type: research

Pharmacological characterization, cellular localization and expression profile of NPY receptor subtypes Y2 and Y7 in large yellow croaker, Larimichthys crocea
In this study, we report the cloning and characterization of two neuropeptide Y receptor subtypes, Y2 (NPY2R) and Y7 (NPY7R), in yellow croaker Larimichthys crocea (L. crocea) (LcNPY2R, LcNPY7R). The gene structure, pharmacological characterization, cell location, and tissue expression of these two receptors were explored. The phylogenetic results showed that LcNPY2R and LcNPY7R had typical G protein-coupled receptor profiles, associated with the Y2 subfamily, with coding sequences that are highly conserved in vertebrates. The expression of both LcNPY2R and LcNPY7R could be activated by LcNPY in HEK293 cells. However, trun...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 7, 2019 Category: Molecular Biology Source Type: research

A reproductive role for the nonapeptides vasotocin and isotocin in male zebrafish (Danio rerio)
Publication date: Available online 6 September 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Z. Altmieme, M. Jubouri, K. Touma, G. Coté, M. Fonseca, T. Julian, J.A. MennigenAbstractTwo distinct nonapeptide systems, vasotocin- and oxytocin-related peptides, evolved in vertebrates. Their role in male zebrafish reproduction has not been formally investigated. We hypothesized that the teleost nonapeptides vasotocin and isotocin stimulate male zebrafish reproductive physiology and success by affecting central neuronal and/or peripheral endocrine pathways. Pharmac...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 7, 2019 Category: Molecular Biology Source Type: research

Functional characterization of olfactory receptors in three Dacini fruit flies (Diptera: Tephritidae) that respond to 1-nonanol analogs as components in the rectal glands
In this study, we conducted RNA sequencing analysis of the chemosensory organs of B. latifrons and Z. cucurbitae to identify the genes coding for chemosensory receptors. Because the skeletons of male attractants are different among Dacini fruit fly species, we analyzed phylogenetic relationships of candidate olfactory receptors (ORs) among the three species. We found that the OR phylogeny reflects the taxonomic relationships of the three species. We further characterized functional properties of OR74a in the three Dacini species to the 1-nonanol analogs related to components in the rectal glands. The three OR74a homologs r...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 7, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: November 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 237Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - September 7, 2019 Category: Molecular Biology Source Type: research

Biochemical characterization of recombinant Penaeus vannamei trypsinogen
Publication date: Available online 30 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Martha Guerrero-Olazarán, Mauricio Castillo-Galván, Juan Antonio Gallegos-López, José Antonio Fuentes-Garibay, José María Viader-SalvadóAbstractTrypsinogens are the inactive precursors of trypsins (EC 3.4.21.4), which are digestive serine proteases. Despite knowing the properties of trypsins from Pacific white shrimp, Penaeus vannamei, the biochemical properties of shrimp trypsinogens including activation mechanisms and kinetic...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 31, 2019 Category: Molecular Biology Source Type: research

Sialic acid on avian erythrocytes
Publication date: Available online 30 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Mark D. Jankowski, Scott Glaberman, David B. Kimball, Kirsten J. Taylor-McCabe, Jeanne M. FairAbstractUnderstanding variation in physiological traits across taxa is a central question in evolutionary biology that has wide-ranging implications in biomedicine, disease ecology, and environmental protection. Sialic acid (Sia), and in particular, 5-N-acetylneuraminic acid (Neu5Ac), is chemically bound to galactose and the underlying glycan via α2–3 or α2–6 ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 31, 2019 Category: Molecular Biology Source Type: research

Regulation of triglyceride synthesis by estradiol in the livers of hybrid tilapia (Oreochromis niloticus ♀ × O. aureus ♂)
Publication date: Available online 29 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Xiaojin Zhang, Zhuojun Han, Huan Zhong, Qinglang Yin, Jun Xiao, Fenghua Wang, Yi Zhou, Yongju LuoAbstractEstradiol (E2) is a sex steroid hormone that modulates multiple physiological processes in teleosts. The aim of this study was to explore the role of E2 in the hepatic lipid metabolism of hybrid tilapia. The hybrid tilapias were injected with different concentrations of E2 (0 mg/kg, 10 mg/kg, 25 mg/kg and 50 mg/kg) and ICI 182,780 (ICI) (35 mg/kg) (an E2 receptor...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 31, 2019 Category: Molecular Biology Source Type: research

Oxidative stress and biomarker responses in the Atlantic halibut after long term exposure to elevated CO2 and a range of temperatures
Publication date: Available online 28 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): B. Carney Almroth, K. Bresolin de Souza, E. Jönsson, J. SturveAbstractCO2 emissions from human activities are increasing, resulting in greater rates of change in the oceans, exceeding any other event in geological and historical records over the past 300 million years. Oceans are warming and pH levels are decreasing. Marine organisms will need to respond to multiple stressors and the potential consequences of global change-related effects in fish needs to be investigated...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 29, 2019 Category: Molecular Biology Source Type: research

Genomic organization of the molt-inhibiting hormone gene in the red swamp crayfish Procambarus clarkii and characterization of single-nucleotide polymorphisms associated with growth
Publication date: Available online 28 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Yu Xu, Gang Peng, Mengling Sun, Jiajia Li, Weihui Yan, Jianqing Tang, Jianlin Pan, Zhiqiang XuAbstractMolt-inhibiting hormone (MIH), a neuropeptide synthesized in the eyestalk in crustaceans, is mainly responsible for the molting by negatively controlling the ecdysteroids secretion. Although there are several reports of the isolation and protein sequencing of MIH in the red swamp crayfish, little is known about the nucleotide sequence and gene organization of this neuropeptid...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 29, 2019 Category: Molecular Biology Source Type: research

Hypoxia-induced remodelling of goldfish membranes
This study is the first to reveal that cold-acclimated animals undergo extensive, tissue-specific restructuring of membrane lipids as they reach minimal metabolic rates. However, hypoxia does not affect membrane composition in fish acclimated to 20 °C. The strong membrane response of cold-acclimated fish involves increases in cholesterol abundance (in white muscle and gills) and in fatty acid saturation, mainly caused by a reduction in %22:6 (docosahexaenoic acid in gills and liver). Major ion pumps like Na+/K+-ATPase are known to be inhibited by cholesterol and activated by 22:6. Because ion pumping by membrane-boun...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 28, 2019 Category: Molecular Biology Source Type: research

Genome-wide identification, evolution of ATF/CREB family and their expression in Nile tilapia
Publication date: Available online 26 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Xiao-Shuang Wang, Shuai Zhang, Zhen Xu, Shu-Qing Zheng, Juan Long, De-Shou WangAbstractThe ATF/CREB family of transcription factors represents a large group of basic region-leucine zipper (bZip) proteins that regulate diverse cellular responses. Here we carried out a comprehensive analysis of ATF/CREB family members in 22 representative animal species. The family probably originated from the early diverging metazoan and significantly expanded in vertebrates due to multiple wh...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 28, 2019 Category: Molecular Biology Source Type: research

Muscle-specific gene expression and metabolic enzyme activities in Atlantic salmon Salmo salar L. fry reared under different photoperiod regimes
This study was conducted to characterise the muscle-specific gene expression, energy metabolism level and growth rates of Atlantic salmon Salmo salar L. reared under different photoperiod regimes. The effects of two photoperiod regimes – LD 16:8 (16 h light:8 h dark) and LD 24:0 (24 h light:0 h dark) over a period of 3 months (August to October) on growth, energy metabolism enzyme activities (cytochrome c oxidase, COX; lactate dehydrogenase, LDH; and aldolase) and the gene expression levels of myogenic regulatory factors (MRFs – MyoD1 paralogues (MyoD1a, MyoD1b, MyoD1c), Myf5, MyoG), myostatin paralog...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 28, 2019 Category: Molecular Biology Source Type: research

Enhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploids
Publication date: Available online 25 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Luisa M. Vera, Erik-Jan Lock, Kristen Hamre, Herve Migaud, Daniel Leeming, Douglas R. Tocher, John F. TaylorAbstractPreviously we showed that, for optimum growth, micronutrient levels should be supplemented above current National Research Council (2011) recommendations for Atlantic salmon when they are fed diets formulated with low levels of marine ingredients. In the present study, the impact of graded levels (100, 200, 400%) of a micronutrient package (NP) on vertebral defo...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 26, 2019 Category: Molecular Biology Source Type: research

A comparison of the nutritional physiology and gut microbiome of urban and rural house sparrows (Passer domesticus)
Publication date: Available online 25 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Alice Gadau, Meli'sa S. Crawford, Roman Mayek, Mathieu Giraudeau, Kevin J. McGraw, Corrie M. Whisner, Christina Kondrat-Smith, Karen L. SweazeaAbstractUrbanization influences food quality and availability for many wild species, but our knowledge of the consequences urbanization has on the nutritional physiology of these animals is currently limited. To fill this gap, we captured House Sparrows (Passer domesticus) from rural and urban environments and hypothesized that increas...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 26, 2019 Category: Molecular Biology Source Type: research

Effects of high fat diet on lipid accumulation, oxidative stress and autophagy in the liver of Chinese softshell turtle (Pelodiscus sinensis)
In conclusion, high fat diet could enhance lipid accumulation in the liver and serum, lead to liver injury and oxidative damage, impair liver antioxidant capacity, regulate antioxidant-related signaling molecule expression and activate hepatic autophagy.Graphical abstract (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 26, 2019 Category: Molecular Biology Source Type: research

Estradiol-17β regulates the expression of insulin-like growth factors 1 and 2 via estradiol receptors in spotted scat (Scatophagus argus)
Publication date: Available online 25 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Ke-Wei Zhang, Tian-Li Wu, Hua-Pu Chen, Dong-Neng Jiang, Chun-Hua Zhu, Si-Ping Deng, Yong Zhang, Guang-Li LiAbstractInsulin-like growth factors (Igf1 and Igf2) play a key role in growth and development of vertebrates. In mammals, the expression of IGFs is regulated by estradiol-17β (E2) via estrogen receptors (ESRs). The expression of igfs can also be regulated by E2 in fish, while comparative study of this is still lacking. The present study examined tissue distribution ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 26, 2019 Category: Molecular Biology Source Type: research

Identification and enzymatic characterization of clip domain serine protease in the digestive fluid of the sea hare, Aplysia kurodai
Publication date: Available online 24 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Akihiko Tsuji, Keizo YuasaAbstractClip domain serine proteases (CDSPs) participate in the extracellular signaling cascades of various biological processes such as innate immune responses in invertebrates. CDSP genes have been isolated from numerous invertebrates. Nevertheless, the enzymatic properties of mollusk CDSPs are poorly understood. In the present study, we demonstrated that the amino acid sequences of the trypsin-like serine protease purified from the digestive fluid...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 25, 2019 Category: Molecular Biology Source Type: research

Chitin deacetylase 1 and 2 are indispensable for larval–pupal and pupal–adult molts in Heortia vitessoides (Lepidoptera: Crambidae)
Publication date: Available online 24 August 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular BiologyAuthor(s): Chun-Yan Wang, Jie Cheng, Zi-Hao Lyu, Zhi-Xing Li, Jing-Xiang Chen, Tong LinAbstractHeortia vitessoides Moore is a notorious defoliator of Aquilaria sinensis (Lour.) Gilg trees. Chitin deacetylases (CDAs) catalyze the N-deacetylation of chitin, which is a crucial process for chitin modification. Here, we identified and characterized HvCDA1 and HvCDA2 from H. vitessoides. HvCDA1 and HvCDA2 possess typical domain structures of CDAs and belong to the Group I CDAs. HvCDA1 and HvC...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 25, 2019 Category: Molecular Biology Source Type: research

Regular alteration of protein glycosylation in skeletal muscles of hibernating Daurian ground squirrels (Spermophilus dauricus)
This study aims to investigate the changes in glycan profiles in the fast-twitch extensor digitorum longus (EDL) muscles of Daurian ground squirrels (Spermophilus dauricus) during hibernation as well as the correlation between protein glycosylation and muscle atrophy prevention in hibernating animals. The results showed that there was no significant change in the muscle-to-body mass ratio, muscle fiber cross-sectional area (CSA), fiber distribution and ultrastructures in the EDL muscles of ground squirrels during hibernation. Alterations of six glycans comprising sialic acid α2–3 galactose (Sia2–3Gal) and...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 25, 2019 Category: Molecular Biology Source Type: research

Identification, expression analysis, and antibacterial activity of Apolipoprotein A-I from amphioxus (Branchiostoma belcheri)
In this study, an ApoA-I gene named BbApoA-I having an open reading frame of 1466 bp and encoding a polypeptide of 345 amino acids was cloned from a cephalochordate (Branchiostoma belcheri). The predicted polypeptide revealed conserved features, including α-helical secondary structures and coiled-coil regions in known vertebrate ApoA-I sequences. Quantitative real-time PCR analysis indicated that BbApoA-I was detected in all of the tested tissues, and the highest expression was found in the hepatic cecum. The BbApoA-I expression was upregulated after the specimens were injected with lipopolysaccharide. Recombinant ...
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 25, 2019 Category: Molecular Biology Source Type: research

Editorial Board
Publication date: October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 236Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 16, 2019 Category: Molecular Biology Source Type: research

Publisher's Note
Publication date: October 2019Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, Volume 236Author(s): (Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology)
Source: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - August 16, 2019 Category: Molecular Biology Source Type: research