Scutellaria baicalensis Georgi alleviates Clostridium difficile associated diarrhea and its modulatory effects on the gut microbiota
Fitoterapia. 2024 Apr 23:105973. doi: 10.1016/j.fitote.2024.105973. Online ahead of print.ABSTRACTThe growing incidence of Clostridium difficile associated diarrhea (CDAD) underscores the urgency for potent treatments. This research delves into the therapeutic potential of Scutellaria baicalensis Georgi (Lamiaceae) root (SR) in addressing CDAD and its influence on gut microbiota. Using a CDAD mouse model and fidaxomicin as a control, SR's impact was measured through diarrhea symptoms, colonic histopathology, and C. difficile toxin levels. Employing the PacBio platform, 16S rRNA full-length gene sequencing analyzed the gut ...
Source: Fitoterapia - April 25, 2024 Category: Biochemistry Authors: Ze-Wei Tang Cong-En Zhang Fu-Zhi Ma Yu-Tao Cui Rui-Han Ye Shi-Biao Pu Zhi-Jie Ma Source Type: research
Reduction of product composition variability using pooled microbiome ecosystem therapy and consequence in two infectious murine models
In this study, we compared the metagenomic profile of pooled products to corresponding single donor-derived products. We demonstrated that pooled products are more homogeneous, diverse, and enriched in beneficial bacteria known to produce anti-inflammatory short chain fatty acids compared to single donor-derived products. We then evaluated pooled products' efficacy compared to corresponding single donor-derived products in Salmonella and C. difficile infectious mouse models. We were able to demonstrate that pooled products decreased pathogenicity by inducing a structural change in the intestinal microbiota composition. Sin...
Source: Cancer Control - April 23, 2024 Category: Cancer & Oncology Authors: Julie Reygner Johanne Delannoy Marie-Th érèse Barba-Goudiaby Cyrielle Gasc Beno ît Levast Enora Gaschet Laurent Ferraris St éphane Paul Nathalie Kapel Anne-Judith Waligora-Dupriet Frederic Barbut Muriel Thomas Carole Schwintner Bastien Laperrousaz Nat Source Type: research
Reduction of product composition variability using pooled microbiome ecosystem therapy and consequence in two infectious murine models
In this study, we compared the metagenomic profile of pooled products to corresponding single donor-derived products. We demonstrated that pooled products are more homogeneous, diverse, and enriched in beneficial bacteria known to produce anti-inflammatory short chain fatty acids compared to single donor-derived products. We then evaluated pooled products' efficacy compared to corresponding single donor-derived products in Salmonella and C. difficile infectious mouse models. We were able to demonstrate that pooled products decreased pathogenicity by inducing a structural change in the intestinal microbiota composition. Sin...
Source: Applied and Environmental Microbiology - April 23, 2024 Category: Microbiology Authors: Julie Reygner Johanne Delannoy Marie-Th érèse Barba-Goudiaby Cyrielle Gasc Beno ît Levast Enora Gaschet Laurent Ferraris St éphane Paul Nathalie Kapel Anne-Judith Waligora-Dupriet Frederic Barbut Muriel Thomas Carole Schwintner Bastien Laperrousaz Nat Source Type: research
Molecules, Vol. 29, Pages 1908: Volatile Fatty Acids Effective as Antibacterial Agents against Three Enteric Bacteria during Mesophilic Anaerobic Incubation
T. Semple
The antibacterial effects of a selection of volatile fatty acids (acetic, propionic, butyric, valeric, and caproic acids) relevant to anaerobic digestion were investigated at 1, 2 and 4 g/L. The antibacterial effects were characterised by the dynamics of Enterococcus faecalis NCTC 00775, Escherichia coli JCM 1649 and Klebsiella pneumoniae A17. Mesophilic anaerobic incubation to determine the minimum bactericidal concentration (MBC) and median lethal concentration of the VFAs was carried out in Luria Bertani broth at 37 °C for 48 h. Samples collected at times 0, 3, 6, 24 and 48 h were used to moni...
Source: Molecules - April 23, 2024 Category: Chemistry Authors: Saanu Victoria Otite Alfonso Jos é Lag-Brotons Lawrence I. Ezemonye Alastair D. Martin Roger W. Pickup Kirk T. Semple Tags: Article Source Type: research
Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae
Chembiochem. 2024 Apr 22:e202400212. doi: 10.1002/cbic.202400212. Online ahead of print.ABSTRACTThe β-hemolytic factor streptolysin S (SLS) is an important linear azol(in)e-containing peptide (LAP) that contributes significantly to the virulence of Streptococcus pyogenes. Despite its discovery 85 years ago, SLS has evaded structural characterizing owing to its notoriously problematic physicochemical properties. Here, we report the discovery and characterization of a structurally analogous hemolytic peptide from Enterococcus caccae, termed enterolysin S (ELS). Through heterologous expression, site-directed mutagenesis, che...
Source: Chembiochem - April 22, 2024 Category: Biochemistry Authors: Chengyou Shi Varshal A Patel Douglas A Mitchell Huimin Zhao Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Enterolyin S, a Polythiazole-containing Hemolytic Peptide from Enterococcus caccae
Chembiochem. 2024 Apr 22:e202400212. doi: 10.1002/cbic.202400212. Online ahead of print.ABSTRACTThe β-hemolytic factor streptolysin S (SLS) is an important linear azol(in)e-containing peptide (LAP) that contributes significantly to the virulence of Streptococcus pyogenes. Despite its discovery 85 years ago, SLS has evaded structural characterizing owing to its notoriously problematic physicochemical properties. Here, we report the discovery and characterization of a structurally analogous hemolytic peptide from Enterococcus caccae, termed enterolysin S (ELS). Through heterologous expression, site-directed mutagenesis, che...
Source: Chembiochem - April 22, 2024 Category: Biochemistry Authors: Chengyou Shi Varshal A Patel Douglas A Mitchell Huimin Zhao Source Type: research
Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
CONCLUSION: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.PMID:38645468 | PMC:PMC11032122 | DOI:10.2147/NSA.S457124 (Source: Appl Human Sci)
Source: Appl Human Sci - April 22, 2024 Category: Physiology Authors: Agata Lange Arkadiusz Matuszewski Marta Kutwin Agnieszka Ostrowska S ławomir Jaworski Source Type: research
Endocarditis: A Review of Recent Literature
AbstractPurpose of ReviewEndocarditis remains a challenging diagnosis, with significant implications for early identification and initiation of therapy. In this review, we examine the evolution in the epidemiology and presentation of infectious endocarditis (IE), the role of new diagnostic tools, and the approach to therapy.Recent FindingsStaphylococcus andStreptococcus species remain the most common causative organisms, but the prevalence of IE caused by enterococcus and non-HACEK organisms is increasing. While newer antibiotics such as dalbavancin have shown promise, treatment must still be tailored on an individual basi...
Source: Current Emergency and Hospital Medicine Reports - April 22, 2024 Category: Emergency Medicine Source Type: research
Mitigating antimicrobial resistance, an approach to stewardship in canine urinary tract infection
AbstractUrinary tract infection (UTI) caused by antimicrobial resistant bacteria is common in dogs leading to serious health impact in pet animal as well as on human health. Understanding the prevalent uropathogens and their drug susceptibility is essential for limiting the antimicrobial resistance through implementation of stewardship policies. In view of this, present study was envisaged to determine the prevalent bacterial uropathogens and their antibiogram from clinical cases of canine UTI. Urine samples were collected from 35 dogs presented with clinical signs of UTI and a total of 27 bacterial isolates were recovered...
Source: Veterinary Research Communications - April 22, 2024 Category: Veterinary Research Source Type: research
Lactic acid bacteria reduce bacterial diarrhea in rabbits via enhancing immune function and restoring intestinal microbiota homeostasis
In this study, Enterococcus faecium ZJUIDS-R1 and Ligilactobaciiius an... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - April 20, 2024 Category: Veterinary Research Authors: Huimin Li, Chaoliang Leng, Nan Chen, Qinchao Ding, Yizhao Yuan, Yilei Zheng, Ge Zhu, Chen Chen, Lichang Xu, Jiangbing Shuai, Qinting Jiang, Daxi Ren and Huanan Wang Tags: Research Source Type: research
Association of Vancomycin AUC/MIC and Trough Concentration With Early Clinical Response in Enterococcus or Coagulase-Negative Staphylococcus Infection: A Prospective Study
This study was condcuted to examine the association of area under the curve (AUC)/minimum inhibitory concentration (MIC) and trough concentration (Ctrough) of vancomycin with treatment outcome and nephrotoxicity in infections caused by Enterococcus spp. and coagulase-negative Staphylococci (CoNS). Peak and trough concentrations were used to calculate AUC in 89 patients receiving vancomycin for infections with Enterococcus spp. (n = 65) or CoNS (n = 24). Correlations between Ctrough, AUC/MIC, early clinical response (ECR), and nephrotoxicity were assessed and cutoff values were determined. Sixty-three (70.8%) patients showe...
Source: The Journal of Clinical Pharmacology - April 19, 2024 Category: Drugs & Pharmacology Authors: Chuleephorn Pitayakittiwong Pakawadee Sermsappasuk Atibordee Meesing Siriluk Jaisue Source Type: research
Association of Vancomycin AUC/MIC and Trough Concentration With Early Clinical Response in Enterococcus or Coagulase-Negative Staphylococcus Infection: A Prospective Study
This study was condcuted to examine the association of area under the curve (AUC)/minimum inhibitory concentration (MIC) and trough concentration (Ctrough) of vancomycin with treatment outcome and nephrotoxicity in infections caused by Enterococcus spp. and coagulase-negative Staphylococci (CoNS). Peak and trough concentrations were used to calculate AUC in 89 patients receiving vancomycin for infections with Enterococcus spp. (n = 65) or CoNS (n = 24). Correlations between Ctrough, AUC/MIC, early clinical response (ECR), and nephrotoxicity were assessed and cutoff values were determined. Sixty-three (70.8%) patients showe...
Source: The Journal of Clinical Pharmacology - April 19, 2024 Category: Drugs & Pharmacology Authors: Chuleephorn Pitayakittiwong Pakawadee Sermsappasuk Atibordee Meesing Siriluk Jaisue Source Type: research
Interaction of selected alkoxy naringenin oximes with model and bacterial membranes
Biomed Pharmacother. 2024 Apr 16;174:116581. doi: 10.1016/j.biopha.2024.116581. Online ahead of print.ABSTRACTNaringenin is a flavonoid found in many fruits and herbs, most notably in grapefruits. In recent years, this compound and its derivatives have been of great interest due to their high biological activity, including fungicidal and bactericidal effects, also in relation to multidrug-resistant bacteria. Membrane interactions of naringenin oxime (NO) and its 7-O-alkyl (7-alkoxy) derivatives, such as methyl (7MENO), ethyl (7ETNO), isopropyl (7IPNO), n-butyl (7BUNO) and n-pentyl (7PENO) were studied. Thermotropic propert...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 18, 2024 Category: Drugs & Pharmacology Authors: Olga Weso łowska Anna Duda-Madej Maria B łaszczyk Kamila Środa-Pomianek Joanna Koz łowska Miros ław Anioł Source Type: research
Interaction of selected alkoxy naringenin oximes with model and bacterial membranes
Biomed Pharmacother. 2024 Apr 16;174:116581. doi: 10.1016/j.biopha.2024.116581. Online ahead of print.ABSTRACTNaringenin is a flavonoid found in many fruits and herbs, most notably in grapefruits. In recent years, this compound and its derivatives have been of great interest due to their high biological activity, including fungicidal and bactericidal effects, also in relation to multidrug-resistant bacteria. Membrane interactions of naringenin oxime (NO) and its 7-O-alkyl (7-alkoxy) derivatives, such as methyl (7MENO), ethyl (7ETNO), isopropyl (7IPNO), n-butyl (7BUNO) and n-pentyl (7PENO) were studied. Thermotropic propert...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 18, 2024 Category: Drugs & Pharmacology Authors: Olga Weso łowska Anna Duda-Madej Maria B łaszczyk Kamila Środa-Pomianek Joanna Koz łowska Miros ław Anioł Source Type: research
