Enterococcus casseliflavus regulates amino acid metabolism in edible insect Clanis bilineata tsingtauica: a functional metagenomics study
This study not only extends our knowledge of gut microbiome in soybean hawkmoths at the species level, but also provides an initial investigation of gene functionality in interaction with insect hosts. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - March 25, 2024 Category: Microbiology Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
Protective effects of different lyoprotectants on survival of clinical bacterial isolates in a hospital biobank
In this study, we collected and characterized Gram-negative and Gram-positive bacteria carrying important antibiotic resistance markers from different clinical samples of hospitalized children. Sucrose (10%), skimmed milk (10%), skimmed milk plus sodium glutamate (10% + 1%), and bovine serum albumin (BSA, 10%) were used as lyoprotectants during the freeze-drying procedure. The survival rate of the lyophilized samples was calculated by dilution plating and measuring the colony forming unit (CFU) after 3 months of storage. The culture analysis results indicated that 25 of the 27 studied bacterial genera (Dilutions 10-3 to 10...
Source: Cryobiology - March 24, 2024 Category: Biology Authors: Masoud Alebouyeh Nasim Almasian Tehrani Fatemeh Fallah Leila Azimi Mehrzad Sadredinamin Neda Yousefi Ghazale Ghandchi Mostafa Haji Molla Hoseini Source Type: research
The neutralizing effect of heparin on blood-derived antimicrobial compounds: impact on antibacterial activity and inflammatory response
In this study, we aimed to investigate the influence of unfractionated heparin, a polyanionic drug clinically used as anticoagulant, on the endotoxin-neutralizing and antibacterial activity of blood-derived AMCs. Serum samples from healthy donors were pre-incubated with increasing concentrations of heparin for different time periods and tested against pathogenic bacteria (Acinetobacter baumannii, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus) and endotoxins from E. coli, K. pneumoniae, and P. aeruginosa. Heparin dose-dependently decreased the activity of blood-...
Source: Frontiers in Immunology - March 22, 2024 Category: Allergy & Immunology Source Type: research
Genomic analysis of inter-hospital transmission of vancomycin-resistant < em > Enterococcus faecium < /em > sequence type 80 isolated during an outbreak in Hiroshima, Japan
This study focused on a vancomycin-resistant enterococcus outbreak that occurred between 2018 and 2021 across two cities in Hiroshima, Japan. The study involved genetic and phylogenetic analyses using whole-genome sequencing of 103 isolates of vancomycin-resistant enterococci to identify the source and transmission routes of the outbreak. Phylogenetic analysis was performed using core genome multilocus sequence typing and core single-nucleotide polymorphisms; infection routes between hospitals were inferred using BadTrIP. The outbreak was caused by Enterococcus faecium sequence type (ST) 80 carrying the vanA plasmid, which...
Source: Antimicrobial Agents and Chemotherapy - March 20, 2024 Category: Microbiology Authors: Takaya Segawa Kanako Masuda Junzo Hisatsune Kasumi Ishida-Kuroki Yo Sugawara Masao Kuwabara Hideki Nishikawa Takahiro Hiratsuka Tatsuaki Aota Yasuo Tao Yoshimi Iwahashi Kuniko Ueda Kaori Mae Ken Masumoto Hiroki Kitagawa Hitoshi Komatsuzawa Hiroki Ohge Mot Source Type: research
Vancomycin heteroresistance caused by unstable tandem amplifications of the < em > vanM < /em > gene cluster on linear conjugative plasmids in a clinical < em > Enterococcus faecium < /em >
Antimicrob Agents Chemother. 2024 Mar 20:e0115923. doi: 10.1128/aac.01159-23. Online ahead of print.ABSTRACTVancomycin heteroresistance is prone to missed detection and poses a risk of clinical treatment failure. We encountered one clinical Enterococcus faecium strain, SRR12, that carried a complete vanM gene cluster but was determined as susceptible to vancomycin using the broth microdilution method. However, distinct subcolonies appeared within the clear zone of inhibition in the E-test assay, one of which, named SRR12-v1, showed high-level resistance to vancomycin. SRR12 was confirmed as heteroresistant to vancomycin us...
Source: Antimicrobial Agents and Chemotherapy - March 20, 2024 Category: Microbiology Authors: Lingyan Sun Hemu Zhuang Mengzhen Chen Yan Chen Yiyi Chen Keren Shi Yunsong Yu Source Type: research
Snakebite-Associated Infections: A Systematic Review and Meta-Analysis
The objective of this systematic review was to determine the prevalence of snakebite infections and characterize the bacteria isolated from these infections. A systematic literature review in five databases was carried out to assess the prevalence of snakebite infection. A meta-analysis was performed using a random-effects model to calculate the pooled prevalence and 95% CIs. Cochran's Q test and the I2 statistic were used to assess between-study heterogeneity. The pooled prevalence of infection due to snakebite was 27.0% (95% CI: 22.0-32.0%), with high heterogeneity among studies (I2 = 99.7%). The prevalence was higher in...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: D Katterine Bonilla-Aldana Jorge Luis Bonilla-Aldana Juan R Ulloque-Badaracco Ali Al-Kassab-C órdova Enrique A Hernandez-Bustamante Esteban A Alarcon-Braga Abdelmonem Siddiq Vicente A Benites-Zapata Alfonso J Rodriguez-Morales Camila Luna Jose A Suarez Source Type: research
Snakebite-Associated Infections: A Systematic Review and Meta-Analysis
The objective of this systematic review was to determine the prevalence of snakebite infections and characterize the bacteria isolated from these infections. A systematic literature review in five databases was carried out to assess the prevalence of snakebite infection. A meta-analysis was performed using a random-effects model to calculate the pooled prevalence and 95% CIs. Cochran's Q test and the I2 statistic were used to assess between-study heterogeneity. The pooled prevalence of infection due to snakebite was 27.0% (95% CI: 22.0-32.0%), with high heterogeneity among studies (I2 = 99.7%). The prevalence was higher in...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: D Katterine Bonilla-Aldana Jorge Luis Bonilla-Aldana Juan R Ulloque-Badaracco Ali Al-Kassab-C órdova Enrique A Hernandez-Bustamante Esteban A Alarcon-Braga Abdelmonem Siddiq Vicente A Benites-Zapata Alfonso J Rodriguez-Morales Camila Luna Jose A Suarez Source Type: research
Effects of polymerization types on plastics ingestion and biodegradation by Zophobas atratus larvae, and successions of both gut bacterial and fungal microbiomes
Environ Res. 2024 Mar 18;251(Pt 2):118677. doi: 10.1016/j.envres.2024.118677. Online ahead of print.ABSTRACTRecent studies demonstrated that plastic degradation in Zophobas atratus superworms is related to the gut microbiota. To determine whether the biodegradation and gut-microbiota were influenced by ingested plastic polymerization types, foams of polypropylene (PP), polyurethane (PU) and ethylene vinyl acetate (EVA) were selected as representatives of polyolefins, polyester and copolymers, and the sole feedstock for superworms for 45 d. Both growth and survival rates of superworms were influenced by the type of plastic ...
Source: Environmental Research - March 20, 2024 Category: Environmental Health Authors: Yue Weng Xiaoyu Han Huayang Sun Jiaming Wang Yumeng Wang Xin Zhao Source Type: research
Genomic analysis of inter-hospital transmission of vancomycin-resistant < em > Enterococcus faecium < /em > sequence type 80 isolated during an outbreak in Hiroshima, Japan
This study focused on a vancomycin-resistant enterococcus outbreak that occurred between 2018 and 2021 across two cities in Hiroshima, Japan. The study involved genetic and phylogenetic analyses using whole-genome sequencing of 103 isolates of vancomycin-resistant enterococci to identify the source and transmission routes of the outbreak. Phylogenetic analysis was performed using core genome multilocus sequence typing and core single-nucleotide polymorphisms; infection routes between hospitals were inferred using BadTrIP. The outbreak was caused by Enterococcus faecium sequence type (ST) 80 carrying the vanA plasmid, which...
Source: Antimicrobial Agents and Chemotherapy - March 20, 2024 Category: Microbiology Authors: Takaya Segawa Kanako Masuda Junzo Hisatsune Kasumi Ishida-Kuroki Yo Sugawara Masao Kuwabara Hideki Nishikawa Takahiro Hiratsuka Tatsuaki Aota Yasuo Tao Yoshimi Iwahashi Kuniko Ueda Kaori Mae Ken Masumoto Hiroki Kitagawa Hitoshi Komatsuzawa Hiroki Ohge Mot Source Type: research
Vancomycin heteroresistance caused by unstable tandem amplifications of the < em > vanM < /em > gene cluster on linear conjugative plasmids in a clinical < em > Enterococcus faecium < /em >
Antimicrob Agents Chemother. 2024 Mar 20:e0115923. doi: 10.1128/aac.01159-23. Online ahead of print.ABSTRACTVancomycin heteroresistance is prone to missed detection and poses a risk of clinical treatment failure. We encountered one clinical Enterococcus faecium strain, SRR12, that carried a complete vanM gene cluster but was determined as susceptible to vancomycin using the broth microdilution method. However, distinct subcolonies appeared within the clear zone of inhibition in the E-test assay, one of which, named SRR12-v1, showed high-level resistance to vancomycin. SRR12 was confirmed as heteroresistant to vancomycin us...
Source: Antimicrobial Agents and Chemotherapy - March 20, 2024 Category: Microbiology Authors: Lingyan Sun Hemu Zhuang Mengzhen Chen Yan Chen Yiyi Chen Keren Shi Yunsong Yu Source Type: research
Snakebite-Associated Infections: A Systematic Review and Meta-Analysis
The objective of this systematic review was to determine the prevalence of snakebite infections and characterize the bacteria isolated from these infections. A systematic literature review in five databases was carried out to assess the prevalence of snakebite infection. A meta-analysis was performed using a random-effects model to calculate the pooled prevalence and 95% CIs. Cochran's Q test and the I2 statistic were used to assess between-study heterogeneity. The pooled prevalence of infection due to snakebite was 27.0% (95% CI: 22.0-32.0%), with high heterogeneity among studies (I2 = 99.7%). The prevalence was higher in...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: D Katterine Bonilla-Aldana Jorge Luis Bonilla-Aldana Juan R Ulloque-Badaracco Ali Al-Kassab-C órdova Enrique A Hernandez-Bustamante Esteban A Alarcon-Braga Abdelmonem Siddiq Vicente A Benites-Zapata Alfonso J Rodriguez-Morales Camila Luna Jose A Suarez Source Type: research
