4-Hydroxy-pyran-2-one and 3-hydroxy-N-methyl-2-oxindole derivatives of Salinispora arenicola from Brazilian marine sediments.
a ODL Abstract Three new 3-hydroxy-N-methyl-2-oxindole (1 and 2) and 4-hydroxy-pyran-2-one (3) derivatives, along with the known 3-hydroxy-N-methyl-2-oxindole (4) and 6-methoxy-N-methylisatin (5) were isolated from a marine Salinispora arenicola strain from sediments of the St. Peter and St. Paul Archipelago, Brazil. The structures of the new compounds were elucidated by a combination of spectroscopic (1D and 2D NMR and HR-ESIMS) data, including single-crystal X-ray diffraction analysis for 2 and 3. Compounds 1 to 5 were assayed for their antimicrobial properties, but only 4 and 5 were active against Enterococcus ...
Source: Fitoterapia - September 12, 2019 Category: Biochemistry Authors: da Silva AB, Pinto FCL, Silveira ER, Costa-Lotufo LV, Costa WS, Ayala AP, Canuto KM, Barros AB, Araújo AJ, Marinho Filho JDB, Pessoa ODL Tags: Fitoterapia Source Type: research
Diverse metabolites from corn silk with anti-A β1-42 aggregation activity.
Diverse metabolites from corn silk with anti-Aβ1-42 aggregation activity. Fitoterapia. 2019 Sep 11;:104356 Authors: Zhou WY, Lou LL, Guo R, Xi YF, Lin B, Huang XX, Song SJ Abstract Five pairs of enantiomeric compounds (1a/1b-5a/5b) including seven undescribed compounds (1a/1b-3a/3b, 4b) along with two known ones (6 and 7) have been isolated from corn silk (Stigma maydis). Racemic mixtures of these enantiomers were separated by HPLC with different types of chiral columns. Their structures were elucidated based on comprehensive spectroscopic analyses together with quantum chemical calculations of 1...
Source: Fitoterapia - September 11, 2019 Category: Biochemistry Authors: Zhou WY, Lou LL, Guo R, Xi YF, Lin B, Huang XX, Song SJ Tags: Fitoterapia Source Type: research
Oxygenated C17 polyacetylene metabolites from Algerian Eryngium tricuspidatum L. roots: Structure and biological activity.
Abstract The secondary metabolite pattern of Eryngium tricuspidatum has been found to be dominated by C17 acetylene oxylipins, according to the chemistry reported in the literature for the genus Eryngium. Two new oxylipins, 11-acetoxy-falcarindiol (4) and 1,2-dihydro-11-acetoxy-falcarindiol (5) have been isolated, along with main related polyacetylenes 1-3 and the already known monoterpene aldehydes 6-10, from the petroleum ether extract of roots. The structure and the absolute configuration of compounds 4 and 5 have been determined by spectroscopic methods as well as by comparison with related known compounds. Po...
Source: Fitoterapia - September 11, 2019 Category: Biochemistry Authors: Djebara A, Ciavatta ML, Mathieu V, Colin M, Bitam F, Carbone M, Gavagnin M Tags: Fitoterapia Source Type: research
A new cerebroside from cordyceps militaris with anti-PTP1B activity.
Abstract Cordyceps militaris (L.) Link (C. militaris) has been used as a folk medicine for treatment of various diseases in China and some other countries. Recent evidence suggests that aqueous extracts of C. militaris have hypoglycemic activity. So the aim of this study was to isolate and characterize compounds with aiti-PTP1B (protein tyrosine phosphatase 1B) activity from C. militaris. As a result, cordycerebroside B (1) together with other three known cerebrosides (2-4) and a disaccharide (5) were isolated by isolated by silica gel column chromatography and semi-preparative high performance liquid chromatograp...
Source: Fitoterapia - August 31, 2019 Category: Biochemistry Authors: Sun J, Xu J, Wang S, Hou Z, Lu X, An L, Du P Tags: Fitoterapia Source Type: research
Assignment of the stereostructures of sesquiterpenoids from the roots of Daphne genkwa via quantum chemical calculations.
Abstract Five new guaiane-type sesquiterpenoids were obtained from the roots of Daphne genkwa. Their gross structures were established by extensive spectroscopic analyses. Attempts on the assignment of the relative configurations were unsuccessful when based on the NOESY correlations. Therefore, NMR chemical shift calculations based on the gauge independent atomic orbital (GIAO) method in combination with the statistical method DP4+ were employed to establish their relative configurations. Furthermore, the absolute configurations were determined by comparing the experimental and calculated electronic circular dich...
Source: Fitoterapia - August 30, 2019 Category: Biochemistry Authors: Wang J, Ren Q, Zhang YY, Guo R, Lin B, Huang XX, Song SJ Tags: Fitoterapia Source Type: research
New sesquiterpenoids from the stems of Dendrobium nobile and their neuroprotective activities.
Abstract Three new sesquiterpenoids, (+)-(1R,2S,3R,4S,5R,6S,9R)-3,11,12-trihydroxypicrotoxane-2(15)-lactone (1), (-)-(1S,2R,3S,4R,5S,6R,9S,12R)-3,11,13-trihydroxypicrotoxane-2(15)-lactone (2), and (+)-(1R,5R,6S,8R,9R)-8,12-dihydroxy-copacamphan-3-en-2-one (3), together with five known compounds, were isolated from the n-butanol soluble fraction of a 95% EtOH extract of the stems of Dendrobium nobile. Their structures were determined by extensive spectroscopic analysis. Particularly, to solve difficult stereochemical problems, electronic circular dichroism calculations, NMR data calculations, and a single-crystal X...
Source: Fitoterapia - August 30, 2019 Category: Biochemistry Authors: Ma C, Meng CW, Zhou QM, Peng C, Liu F, Zhang JW, Zhou F, Xiong L Tags: Fitoterapia Source Type: research
Antimicrobial drimane - phthalide derivatives from Hypoxylon fendleri BCC32408.
Abstract Fourteen new compounds including thirteen drimane - phthalide derivatives (fendlerals A - C, fendlerins A - D, fendlerols A - B, fendleric acids A - C, fendlerinine G) and one terphenyl derivative (fendleryl E) along with eight known compounds, fendlerinine A, rickenyls C - D, fendleryls C - D, atromentin, tetramethyl atromentin, and (±)-microsphaerophthalide F, were isolated from the wood fungus Hypoxylon fendleri BCC32408. Compared with the prior work, the results indicated the agitation effect on the production of bioactive drimane - phthalides. The chemical structures were determined based upon...
Source: Fitoterapia - August 30, 2019 Category: Biochemistry Authors: Intaraudom C, Punyain W, Bunbamrung N, Dramae A, Boonruangprapa T, Pittayakhajonwut P Tags: Fitoterapia Source Type: research
Antibacterial stilbenes from the tubers of Bletilla striata.
Abstract Three new bibenzyl derivatives (bletstrins A-C, 1-3), including two bibenzyls that have hydroxyl-substituted chiral centers on the aliphatic bibenzyl bridge, along with eighteen known stilbenoids (4-21) were isolated from the tubers of Bletilla striata. The structures of new compounds were elucidated by the use of 1D/2D NMR spectroscopic data. The absolute configurations of bletitrins A and B were determined by optical rotation value. Compounds 13-16 were isolated from the Orchidaceae for the first time. Most of the isolated compounds were evaluated for their antibacterial activities against three gram-po...
Source: Fitoterapia - August 30, 2019 Category: Biochemistry Authors: Jiang S, Chen CF, Ma XP, Wang MY, Wang W, Xia Y, Zhang N, Wu MK, Pan WD Tags: Fitoterapia Source Type: research
Monoterpenoid indole alkaloids from the bark of Melodinus henryi.
Abstract Four new alkaloids, melodinines W1-W4 (1-4), together with twenty one known alkaloids (5-25) were isolated from Melodinus henryi. The structures with absolute configurations were elucidated by extensive MS and NMR spectroscopic methods, as well as the single crystal X-ray diffraction and ECD calculations. All compounds were evaluated for their cytotoxicities to five human cancer cell lines. Many compounds showed certain cytotoxicities to five human cancer cell lines with an IC50 range of 1.4-29.4 μM. PMID: 31473334 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - August 30, 2019 Category: Biochemistry Authors: He J, Zhang FL, Li ZH, Yang HX, Shao Q, Feng T, Liu JK Tags: Fitoterapia Source Type: research
Review: Molecular techniques to assess genetic variation within and between Panax ginseng and Panax quinquefolius.
Abstract A variety of methods have been used to examine genetic differences in P. ginseng and P. quinquefolius. They have shown genetic differences within populations of P. ginseng (within and between elite cultivars, landraces and wild accessions), within populations of P. quinquefolius (within and between wild and cultivated accessions) and between P. ginseng and P. quinquefolius as well as other Panax species. Some examples of their applications have been to show that some elite cultivars are not uniform, there are possible founder effects in certain populations, there has been the spread of cultivated types in...
Source: Fitoterapia - August 28, 2019 Category: Biochemistry Authors: Goodwin PH, Proctor E Tags: Fitoterapia Source Type: research
Pharmacokinetic of gastrodigenin rhamnopyranoside from Moringa seeds in rodents.
Abstract Gastrodigenin rhamnopyranoside (GR) is a hepatoprotective compound that exists in Moringa oleifera seeds. However, the UPLC-MS/MS method for the determination of GR (in-vitro/in-vivo) is lacking clarification. Herein, this study established the UPLC-MS/MS technique, which was effective and sensitive for the investigation of the pharmacokinetics and biodistribution of GR in rats and mice. The separation was achieved with a Shim-pack XR-ODS III C18 column (2.0 × 75 mm, 1.6 μm) at 40 °C, while the mobile phase (Acetonitrile/0.1% Formic acid =12:82, v/v) was at an eluting rate of 0.2 ...
Source: Fitoterapia - August 27, 2019 Category: Biochemistry Authors: Li W, Sun C, Deng W, Liu Y, Adu-Frimpong M, Yu J, Xu X Tags: Fitoterapia Source Type: research
Five 11 α, 12α-epoxy pentacyclic triterpenoid saponins with antithrombus activities from Glechoma longituba.
Five 11α, 12α-epoxy pentacyclic triterpenoid saponins with antithrombus activities from Glechoma longituba. Fitoterapia. 2019 Aug 27;:104345 Authors: Ouyang XL, Mao WH, Wang CG, Pan YM, Liang D, Wang HS Abstract The present study reports the phytochemical investigation of n-butanol-soluble extracts of Glechoma longituba. Five new oleanane-type triterpenoid saponins with an 11α, 12α-epoxy unit, named glechomanosides A - E, were isolated from the n-butanol soluble fraction of G. longituba. Their chemical structures were established using HRESIMS, IR, 1D NMR, and 2D NMR techniques...
Source: Fitoterapia - August 27, 2019 Category: Biochemistry Authors: Ouyang XL, Mao WH, Wang CG, Pan YM, Liang D, Wang HS Tags: Fitoterapia Source Type: research
LC-MS guided identification of dimeric 2-(2-phenylethyl)chromones and sesquiterpene-2-(2-phenylethyl)chromone conjugates from agarwood of Aquilaria crassna and their cytotoxicity.
Abstract Four previously unreported dimeric 2-(2-phenylethyl)chromones (1-4a) and three unprecedented sesquiterpene-2-(2-phenylethyl)chromone conjugates (5-7), comprising a sesquiterpenoid and a 5,6,7,8-tetrahydroxy-2-(2-phenylethyl)-5,6,7,8 -tetrahydro-4H-chromone moiety, as well as three known dimeric 2-(2-phenylethyl)chromones (4b, 8a, 8b) were identified from agarwood originating from Aquilaria crassna in Cambodia by LC-MS guided separation. Their structures were elucidated by extensive NMR techniques, HRESIMS and CD. All compounds were evaluated for their cytotoxicity against four human cancer cell lines. Com...
Source: Fitoterapia - August 27, 2019 Category: Biochemistry Authors: Xia L, Li W, Wang H, Chen H, Cai C, Yang L, Jiang B, Yang Y, Mei W, Dai H Tags: Fitoterapia Source Type: research
Melotenuines A-E, cytotoxic monoterpenoid indole alkaloids from Melodinus tenuicaudatus.
Abstract Five new monoterpenoid indole alkaloids, melotenuines A-E (1-5), along with 18 known indole alkaloids, were isolated from the twigs and leaves of Melodinus tenuicaudatus. The structures of the new alkaloids were determined by a combination of MS, NMR and ECD analysis. Melotenuine A (1) represents the first example of aspidosperma-meloscandonine type bisindole alkaloids characterized by a methylene bridge between the two monomers, while melotenuine B (2) possessed a rare eburnamine-melsocandonine skeleton. All of the new indole alkaloids were evaluated for in vitro cytotoxicities against five human cancer ...
Source: Fitoterapia - August 27, 2019 Category: Biochemistry Authors: Liu JW, Huo ZQ, Zhao Q, Hao XJ, He HP, Zhang Y Tags: Fitoterapia Source Type: research
Biotransformation of Huperzine A by Irpex lacteus-A fungal endophyte of Huperzia serrata.
Abstract The biotransformation of huperzine A (hupA), one of the characteristic bioactive constituents of the medicinal plant Huperzia serrata, by a fungal endophyte of the host plant was studied. Two previously undescribed compounds 1-2, along with a known analog 8α,15α-epoxyhuperzine A (3), were isolated and identified. The structures of all the isolates were established by spectroscopic methods including NMR, MS, IR, and UV spectra. In particular, the absolute configurations of 1 and 2 were elucidated by CD spectra comparison and theoretic NOE strength calculation. In the LPS-induced neuro-inflammat...
Source: Fitoterapia - August 27, 2019 Category: Biochemistry Authors: Ying YM, Xu YL, Yu HF, Zhang CX, Mao W, Tong CP, Zhang ZD, Tang QY, Zhang Y, Shan WG, Zhan ZJ Tags: Fitoterapia Source Type: research
Two new lignans from the aerial parts of Saururus chinensis with cytotoxicity toward nasopharyngeal carcinoma.
Abstract Two new lignans (1 and 12), together with 15 known compounds (2-11 and 13-17), were isolated from the aerial parts of Saururus chinensis Baill. Their structures were determined through extensive spectroscopic analyses. All the isolates were evaluated for their cytotoxicity against four human nasopharyngeal carcinoma cells (HONE1, CNE1, CNE2, and SUNE1). Compound 13 showed the most potent cytotoxicity toward HONE1, SUNE1, CNE2, and CNE1 cells with IC50 values of 0.76, 5.42, 5.86 and 6.28 μM, respectively. Further studies revealed that compound 13 suppressed cell growth by arresting the cell cycle at t...
Source: Fitoterapia - August 26, 2019 Category: Biochemistry Authors: Cheng Y, Yin Z, Jiang F, Xu J, Chen H, Gu Q Tags: Fitoterapia Source Type: research
Polyprenylated benzophenone derivatives with a novel tetracyclo[8.3.1.03,11.05,10]tetradecane core skeleton from Clusia burle-marxii exhibited cytotoxicity against GL-15 glioblastoma-derived human cell line.
acute; R, Cruz FG Abstract Three new polyprenylated benzophenone derivatives (1-3) were identified in the hexane extract of Clusia burle-marxii trunks, through the isolation and structural elucidation of their methyl derivatives, along with two known polyprenylated benzophenone derivatives sampsonine N (4) and obdeltifolione C (5). Burlemarxiones A (1) and B (2) show an unprecedent tetracyclo[8.3.1.03,11.05,10]tetradecane core skeleton. These compounds are a pair of β-diketones in tautomeric equilibrium, whereas isonemorosonol (3) is the respective β-diketone pair in tautomeric equilibrium with nemoroson...
Source: Fitoterapia - August 26, 2019 Category: Biochemistry Authors: Ferraz CG, Ribeiro PR, Marques ÉJ, Mendonça R, Guedes MLS, Silveira ER, El-Bachá R, Cruz FG Tags: Fitoterapia Source Type: research
Uvarialuridols A-C, three new polyoxygenated cyclohexenes from the twig and leave extracts of Uvaria lurida.
Abstract The first phytochemical investigation of Uvaria lurida resulted in the isolation and identification of three new polyoxygenated cyclohexenes, (+)-(1R,2S,3R,6S)-uvarialuridols A-C (1-3), together with 10 known compounds (4-13). All new structures were elucidated by spectroscopic methods and HRESIMS. The absolute configurations of compounds 1 and 5 were confirmed by X-ray diffraction analysis using Cu Kα radiation. The absolute configurations of compounds 2-4 were identified from comparisons of their specific rotations and ECD spectra with those of known compounds. Compound 11 showed α-glucosida...
Source: Fitoterapia - August 26, 2019 Category: Biochemistry Authors: Suthiphasilp V, Maneerat W, Andersen RJ, Patrick BO, Phukhatmuen P, Pyne SG, Laphookhieo S Tags: Fitoterapia Source Type: research
Atropisomeric bi(9,10-dihydro)phenanthrene and phenanthrene/bibenzyl dimers with cytotoxic activity from the pseudobulbs of Pleione bulbocodioides.
Abstract Bulbocodioidins E-H (1-4), four pairs of undescribed racemic bi(9,10-dihydro)phenanthrene and phenanthrene/bibenzyl atropisomers, along with four known compounds (5-8) were isolated from the ethanol extract of the pseudobulbs of Pleione bulbocodioides. Their structures were established by HRESIMS and comprehensive NMR spectroscopic data analysis. Their absolute configurations were elucidated by comparison of their experimental and calculated ECD (electronic circular dichroism) curves. Furthermore, compound 4a displayed cytotoxic activity against colon cancer (HCT-116), liver cancer (HepG2), and breast can...
Source: Fitoterapia - August 14, 2019 Category: Biochemistry Authors: Wang C, Shao SY, Han SW, Li S Tags: Fitoterapia Source Type: research
Non volatile constituents of the vermouth ingredient Artemisia vallesiaca.
Abstract The Alpine wormwood Artemisia vallesiaca All. was considered the most valuable ingredient of vermouth, a celebrated aromatized wine. A. vallesiaca has a very limited geographical distribution, and the booming market of vermouth decimated its natural population, resulting in the eventual replacement of this rare species with more common and less expensive wormwoods like A. absinthium L.. Over the past years, attempts to revive the original recipe(s) of vermouth have fostered the establishment of cultivations of A. vallesiaca in pre-montane settings. In order to assist these projects, the phytochemical prof...
Source: Fitoterapia - August 13, 2019 Category: Biochemistry Authors: Pollastro F, Lopatriello A, Vouillamoz JF, Appendino G, Taglialatela-Scafati O, Forino M Tags: Fitoterapia Source Type: research
New tricyclic prezizaane sesquiterpenoids from agarwood.
WL, Dai HF Abstract Ten new tricyclic prezizaane types sesquiterpenoids (1-10) were isolated from ethyl ether extract of agarwood originated from Aquilaria sp.. Their structures were unambiguously elucidated on the basis of 1D and 2D NMR spectra as well as by HRESIMS data. The absolute configuration of the new prezizaenes 1, 2 and 4 was determined by single-crystal X-ray diffraction, while TDDFT-ECD method was applied for 6. Compounds 4 and 5 displayed significant inhibitory activities toward α-glucosidase with IC50 values of 0.22 and 1.99 mM, respectively. PMID: 31415800 [PubMed - as supplied by pub...
Source: Fitoterapia - August 12, 2019 Category: Biochemistry Authors: Yang YL, Li W, Wang H, Yang L, Yuan JZ, Cai CH, Chen HQ, Dong WH, Ding XP, Jiang B, Mándi A, Kurtán T, Mei WL, Dai HF Tags: Fitoterapia Source Type: research
8-methoxysmyrindiol from Gerbera piloselloides (L.) Cass. and its vasodilation effects on isolated rat mesenteric arteries.
Abstract Gerbera piloselloides (L.) Cass. (Compositae) possesses various biological effects. It is used as an oriental remedy for relieving cough and resolving phlegm. The present study is to investigate the vasodilation effects of Gerbera piloselloides on isolated rat mesenteric arteries (MAs) and the potential mechanism. Different organic extracts of Gerbera piloselloides were tested, and an HPLC-UV-FD-based analytical method was established to identify the active constituents. The principal components, namely, 8-MOP (8-methoxypsoralan) and 8-MSD (8-methoxysmyrindiol), were found to be predominant in the extract...
Source: Fitoterapia - August 9, 2019 Category: Biochemistry Authors: He F, Yang J, Cheng X, Wang R, Qu H, Jiang H, Bai Y, Cao W Tags: Fitoterapia Source Type: research
Leishmanicidal and antimicrobial activity of primin and primin-containing extracts from Miconia willdenowii.
Abstract As a part of an ongoing bioprospective project, searching for potential medicinal plants from the Brazilian Atlantic Forest, Miconia willdenowii was selected for its potential leishmanicidal and antimicrobial activities. The crude ethanolic extract of M. willdenowii showed an inhibition of 99.7% of the promastigote forms of Leishmania amazonensis at the concentration of 80 μg/mL. Further investigation of its antimicrobial activity against pathogenic fungi and Gram positive and negative bacteria, revealed a significant antimicrobial activity. A bioguided study with its liquid-liquid partition fraction...
Source: Fitoterapia - August 9, 2019 Category: Biochemistry Authors: Viegas FPD, Espuri PF, Oliver JC, Silva NC, Dias ALT, Marques MJ, Soares MG Tags: Fitoterapia Source Type: research
Bioactive constituents study of Pugionium cornutum L. Gaertn on intestinal motility.
Abstract Thirty-one compounds were obtained and identified from the dried roots of Mongolian medicinal and edible plant, Pugionium cornutum L. Gaertn. Eight of them were new ones, and named as pugcornols A (1), B (2), C (3), and D (4), pugcornosides A (5), B (6), C (7), and D (8), respectively. Among them, 1-4 were rare naturally occurring thiohydantoin derivatives. Meanwhile, all the isolates were determined for their activities on isolated mice jejunum contraction, and ten of them increased height of the spontaneous contractions. Moreover, we partly clarified the mechanism of 5-(methylsulfinyl)-pentanenitrile (9...
Source: Fitoterapia - August 8, 2019 Category: Biochemistry Authors: Shi W, Ruan J, Guo Y, Ding Z, Yan J, Qu L, Zheng C, Zhang Y, Wang T Tags: Fitoterapia Source Type: research
Triterpenes from Salvia argentea var. aurasiaca and their antibacterial and cytotoxic activities.
Abstract Five undescribed ursane-type triterpenes, identified as 1β,3β,15α-trihydroxy-urs-9(11)-12-diene (1), 1β,3β,15α,28-tetrahydroxy-urs-9(11),12-diene (2), 1β,3β-dihydroxy-urs-9(11),12-dien-28-al (3), 1β,3β,7β,15α,28-pentahydroxy-urs-12-ene (4), and 1β,3β,15α-trihydroxy-urs-11-en-28-al (5), together with five known compounds (6-10), were isolated from the acetone extract of the dried aerial parts of Salvia argentea L. var. aurasiaca (Pomel) Batt. et Trab. (Lamiaceae). Structures were elucidated on the basis of extensive spectroscopi...
Source: Fitoterapia - August 8, 2019 Category: Biochemistry Authors: Bechkri S, Magid AA, Voutquenne-Nazabadioko L, Djemaa B, Kabouche A, Lehbili M, Lakhal H, Abedini A, Gangloff SC, Morjani H, Kabouche Z Tags: Fitoterapia Source Type: research
Development of species-specific chloroplast markers for the authentication of Gynostemma pentaphyllum and their distribution in the Korean peninsula.
Abstract Gynostemma pentaphyllum is a traditional oriental medicinal herb used as tea since ancient time. Among Gynostemma species, G. pentaphyllum has more active chemical components and better therapeutic effect. It is used to cure depression, diabetes, anxiety, hyperlipidemia, fatigue, immunity, cancer, and oxidative stress. Overexploitation of G. pentaphyllum for its medicinal benefits has been on a rise, due to which they are adulterated or mistakenly identified with other members of Gynostemma species. Hence, we used chloroplast universal regions such as ycf3, accD, petD, psbB and their polymorphism to disti...
Source: Fitoterapia - August 7, 2019 Category: Biochemistry Authors: Abid S, Mohanan P, Kaliraj L, Park JK, Ahn JC, Yang DC Tags: Fitoterapia Source Type: research
Terpenoid and phenolic constituents from the roots of Ilex pubescens.
This study is the first to report monoterpene glycosides with β-pinene aglycone in Aquifoliaceae. Nine of these compounds were evaluated in vitro for their anti-platelet aggregation activities. Among them, compounds 3 and 4 showed moderate inhibitory activities on ADP-induced blood platelet aggregation [inhibition (%): 32.3 and 33.6, respectively] as compared to aspirin. PMID: 31400482 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - August 7, 2019 Category: Biochemistry Authors: Zhou XD, Xu XW, Xi YY, Zhou Y Tags: Fitoterapia Source Type: research
Gut microbial transformation, a potential improving factor in the therapeutic activities of four groups of natural compounds isolated from herbal medicines.
Abstract Herbal medicines (HMs) have attracted widespread attention because of their significant contributions to the prevention and treatment of many human diseases. Recently, gut microbiota has become an important frontier to understand the therapeutic mechanisms of medicines. Gut microbiota-mediated transformation is a microbial metabolic form after oral administrations of HMs compounds. A great number of studies showed that gut microbiota could transform some HMs compounds by the variation of chemical structures into several active metabolites, which exerted better bioavailabilities and therapeutic activities ...
Source: Fitoterapia - August 6, 2019 Category: Biochemistry Authors: Wang J, Feng W, Tang F, Ao H, Peng C Tags: Fitoterapia Source Type: research
Monoterpene glycosides with anti-inflammatory activity from Paeoniae Radix.
Abstract Six new monoterpene glycosides, named 6'-O-nicotinoylalbiflorin (1), 4'-O-vanillylalbiflorin (2), paeonidanin L (3), paeoniflorigenin-1-O-β-d-xyloside (4), 6'-(2-hydroxypropanoyl)-paeoniflorin (5), oxylactiflorin (6), together with 16known ones (7-22) were isolated from the 70% ethanol extract of Paeoniae Radix. Their structures were elucidated based on spectroscopic analysis (1D and 2D NMR, HRESIMS, IR and UV), chemical evidences and comparison with literatures. The inhibitory effects of all the isolates were evaluated against lipopolysaccharide (LPS) stimulated PGE2 production in RAW 264.7 macropha...
Source: Fitoterapia - August 6, 2019 Category: Biochemistry Authors: Li HB, Shi Y, Pang Q, Mei Y, Su Z, Yao XS, Yu Y Tags: Fitoterapia Source Type: research
New eremophilane-type sesquiterpenes and maleimide-bearing compounds from Carpesium abrotanoides L.
, Zou K Abstract Two new eremophilane-type sesquiterpenes, carperemophilanes A and B (1-2), three new maleimide-bearing compounds, carpesiumaleimides A-C (3-5), along with a known sesquiterpene, carabrol (6), were isolated from the ethanol extract of Carpesium abrotanoides L. Their structures were elucidated by analysis of their NMR and MS data as well as by comparison with the literature. The absolute configuration of carperemophilane A (1) was determined by single-crystal X-ray diffraction analysis. All isolated compounds (1-6) were evaluated in vitro for cytotoxicity against two human cancer cell lines MDA-MB-2...
Source: Fitoterapia - August 6, 2019 Category: Biochemistry Authors: Wang L, Jin G, Tian L, Ebrahim W, Höfert SP, Janiak C, Chen JF, Guo ZY, Schäberle TF, Liu Z, Cheng F, Proksch P, Zou K Tags: Fitoterapia Source Type: research
Sesquiterpenoids and γ-pyranone derivatives from the whole plant of Erigeron breviscapus and their neuroprotective effects.
Sesquiterpenoids and γ-pyranone derivatives from the whole plant of Erigeron breviscapus and their neuroprotective effects. Fitoterapia. 2019 Aug 06;:104288 Authors: Han S, Liu Y, Hou Z, Sun X, Yao G, Gao P, Li L, Song S Abstract Four new sesquiterpenes (1-2, 6-7), a new pyranone glycoside (10) along with six known compounds, were isolated from the whole plant of Erigeron breviscapus. Their planar structures were elucidated using extensive spectroscopic analyses. Brevisterpene A (1) and brevisterpene B (2) were proved to be a pair of diastereomer followed by mixtures resolution using chiral HPLC...
Source: Fitoterapia - August 6, 2019 Category: Biochemistry Authors: Han S, Liu Y, Hou Z, Sun X, Yao G, Gao P, Li L, Song S Tags: Fitoterapia Source Type: research
Bioactive sesquiterpenoids and sesquiterpenoid glucosides from the flowers of Inula japonica.
Abstract Three new sesquiterpenoids (1-3) and two new sesquiterpenoid glucosides (4 &5), along with 24 known analogues (6-29), were obtained from the flowers of Inula japonica. Structures of the new compounds were determined by interpretation of spectroscopic data, and their absolute configurations were established via comparison of experimental with computed ECD curves. All the isolates were tested in an in vitro cytotoxic assay against human A549, MCF-7 and MDA-MB-231 cancer cell lines, and selective ones displayed significant activity close to the positive control adriamycin. The new molecules 1-5 were also...
Source: Fitoterapia - August 6, 2019 Category: Biochemistry Authors: Yu ZP, Zhang JS, Zhang Q, Yu SJ, Zhang Y, Yu JH, Zhang H Tags: Fitoterapia Source Type: research
Depsidones and a dihydroxanthenone from the endophytic fungi Simplicillium lanosoniveum (J.F.H. Beyma) Zare & W. Gams PSU-H168 and PSU-H261.
Depsidones and a dihydroxanthenone from the endophytic fungi Simplicillium lanosoniveum (J.F.H. Beyma) Zare & W. Gams PSU-H168 and PSU-H261. Fitoterapia. 2019 Aug 05;:104286 Authors: Rukachaisirikul V, Chinpha S, Saetang P, Phongpaichit S, Jungsuttiwong S, Hadsadee S, Sakayaroj J, Preedanon S, Temkitthawon P, Ingkaninan K Abstract Three new compounds including two depsidones (simplicildones J and K) and one dihydroxanthenone (globosuxanthone E) together with nine known compounds were obtained from the crude extracts of two endophytic fungi Simplicillium lanosoniveum (J.F.H. Beyma) Zare & W. Ga...
Source: Fitoterapia - August 5, 2019 Category: Biochemistry Authors: Rukachaisirikul V, Chinpha S, Saetang P, Phongpaichit S, Jungsuttiwong S, Hadsadee S, Sakayaroj J, Preedanon S, Temkitthawon P, Ingkaninan K Tags: Fitoterapia Source Type: research
Cytotoxic ergosterols from cultures of the basidiomycete Psathyrella candolleana.
Abstract Three newly isolated ergosterols, psathergosterols A-C (1-3), together with two known ones (4 and 5), have been isolated from cultures of the basdiomycete Psathyrella candolleana. Their structures with the absolute configuration were elucidated by means of spectroscopic methods and the single crystal X-ray diffraction. Compounds 2-4 exhibited certain cytotoxicities to five human cancer cell lines (HL-60, SMMC-7721, A-549, MCF-7, SW480). PMID: 31386896 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - August 3, 2019 Category: Biochemistry Authors: Liu YP, Pu CJ, Wang M, He J, Li ZH, Feng T, Xie J, Liu JK Tags: Fitoterapia Source Type: research
The NCI library of traditional Chinese medicinal plant extracts - Preliminary assessment of the NCI-60 activity and chemical profiling of selected species.
Abstract Botanical-based natural products are an important resource for medicinal drug discovery and continue to provide diverse pharmacophores with therapeutic potential against cancer and other human diseases. A prototype Traditional Chinese Medicine (TCM) plant extract library has been established at the US National Cancer Institute, which contains both the organic and aqueous extracts of 132 authenticated medicinal plant species that collectively represent the potential therapeutic contents of most commonly used TCM herbal prescriptions. This library is publicly available in 96- and 384- well plates for high t...
Source: Fitoterapia - August 3, 2019 Category: Biochemistry Authors: He M, Grkovic T, Evans JR, Thornburg CC, Akee RK, Thompson JR, Whitt JA, Harris MJ, Loyal JA, Britt JR, Jia L, White JD, Newman DJ, O'Keefe BR Tags: Fitoterapia Source Type: research
Racemic neolignans from Crataegus pinnatifida: Chiral resolution, configurational assignment, and cytotoxic activities against human hepatoma cells.
Abstract Phytochemical investigation of the fruit of Crataegus pinnatifida led to the isolation of four pairs of dihydrobenzofuran neolignan enantiomers (1a/1b-4a/4b) including six new compounds (1a/1b, 2a/2b, 3a and 4a). The enantioseparations of the racemates were achieved successfully by chiral chromatographic column. Their structures were established by comprehensive spectroscopic analyses and the absolute configurations were determined by quantum mechanical calculation of electronic circular dichroism (ECD) spectra. All compounds were evaluated in vitro for their cytotoxicity using human hepatocellular carcin...
Source: Fitoterapia - August 3, 2019 Category: Biochemistry Authors: Guo R, Lv TM, Shang XY, Yao GD, Lin B, Wang XB, Huang XX, Song SJ Tags: Fitoterapia Source Type: research
Polyketides from Alternaria alternata MT-47, an endophytic fungus isolated from Huperzia serrata.
Abstract Four new polyketides, alternatains A-D (1-4), along with 17 known compounds (5-21) were obtained from the solid substrate fermentation cultures of Alternaria alternata MT-47, an endophytic fungus isolated from the medicinal plant of Huperzia serrata. Their structures were elucidated by extensive spectroscopic and spectrometric techniques (1D and 2D NMR, IR, and HRESIMS) and calculated electronic circular dichroism (ECD) method. Compounds 4, 6, 15, and 21 exhibited inhibitory activities on ATP release of thrombin-activated platelets with IC50 values in the range of 18.2-68.8 μM. PMID: 31381956 [P...
Source: Fitoterapia - August 2, 2019 Category: Biochemistry Authors: Yang H, Qi B, Ding N, Jiang F, Jia F, Luo Y, Xu X, Wang L, Zhu Z, Liu X, Tu P, Shi S Tags: Fitoterapia Source Type: research
Steroidal alkaloid variation in Veratrum californicum as determined by modern methods of analytical analysis.
This study provides insight into optimal harvest time, plant growth stage, harvest location, and plant part required to isolate, yet to be characterized, alkaloids of interest for exploration as Hh pathway antagonists with desirable medicinal properties. PMID: 31381957 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - August 2, 2019 Category: Biochemistry Authors: Turner MW, Rossi M, Campfield V, French J, Hunt E, Wade E, McDougal OM Tags: Fitoterapia Source Type: research
Identification and structure-activity relationship (SAR) of chemical constituents from Daemonorops draco (Willd.) Blume and selected commercial flavonoids on anti-osteoclastogenesis activity.
Abstract Osteoclastogenesis-related bone diseases including osteoporosis, rheumatoid arthritis, Paget's disease and periodontitis are worldwide occurred and cause severe health problems including bone fracture and bone cancer. However, A few studies have shown that Daemonorops draco (Willd.) Blume may decrease bone destruction and relieve bone cancer pain. In this research, we isolated and purified four known and two novel compounds from D. draco and investigated their anti-osteoclastogenesis activity using RAW264.7 cells. Among them, com.1 exhibited the most effective inhibitory activity on osteoclastogenesis wit...
Source: Fitoterapia - July 31, 2019 Category: Biochemistry Authors: Wang X, Batubara I, Yamauchi K, Mitsunaga T Tags: Fitoterapia Source Type: research
Acylation of 25-hydroxyprotopanaxatriol with aromatic acids increases cytotoxicity.
Abstract 20(R)-25-hydroxyprotopanaxadiol (25-OH-PPD) is a natural compound showing a variety of anti-tumor effects. In an attempt to search for a new anti-cancer compound with higher antitumor activities, we designed and synthesized a series of 25-OH-PPD derivatives. Cytotoxicity assay of these derivatives towards MCF-7, A549, U87, HO-8901, Hela cancer cell lines and normal IOSE144 cell lines were tested by MTT assay. Results showed that compared with compound 25-OH-PPD, Compounds 4, 5, 6, 10, 11 showed strong anticancer activity, and all compounds showed low toxicity or no toxicity for normal cells. In particular...
Source: Fitoterapia - July 26, 2019 Category: Biochemistry Authors: Lv L, Lu J, Zhang H, Wang X, Su G, Piao H, Zhao Y Tags: Fitoterapia Source Type: research
Cytotoxic cytochalasans from fungus Xylaria longipes.
Abstract Five new cytochalasans (1-5) were isolated from the rice fermentation of fungus Xylaria longipes, along with seven known compounds cytochalasin P (6), cytochalasin D (7), zygosporin D (8), 7-O-acetylcytochalasin D (9), cytochalasin C (10), 6,7-dihydro-7-oxo-cytochalasin C (11), and 6,7-dihydro-7-oxo-deacetylcytochalasin C (12). Their structures and absolute configurations were determined by extensive experimental spectroscopic methods as well as ECD calculation and GIAO 13C NMR calculation. The cytotoxicity of obtained compounds (1-12) was evaluated against human cancer cell lines HL-60, A549, SMMC-7721, ...
Source: Fitoterapia - July 25, 2019 Category: Biochemistry Authors: Wang WX, Li ZH, He J, Feng T, Li J, Liu JK Tags: Fitoterapia Source Type: research
A natural product atalantraflavone inhibits non-small cell lung cancer progression via destabilizing Twist1.
Abstract The non-small cell lung cancer (NSCLC) represents a malignant type of cancer worldwide. The atalantraflavone (AFL) is a natural product isolated from leaves of Atalantia monophylla (L.) DC. However, the function of atalantraflavone in NSCLC is still elusive. In present work, we have unraveled a novel function of AFL in NSCLC. AFL significantly inhibited NSCLC cell viability and colony formation. AFL increased sub-G1 fraction and apoptotic rates in a dose-dependent manner. Furthermore, Twist-related protein 1 (Twist1) was identified as the target of AFL. The association between AFL and Twist1 markedly decr...
Source: Fitoterapia - July 24, 2019 Category: Biochemistry Authors: Yuan T, Ling F, Wang Y, Teng Y Tags: Fitoterapia Source Type: research
Lycopodium alkaloids from Huperzia serrata.
Abstract Five new Lycopodium alkaloids, huperzine Y1 (1), huperzine Y2 (2), huperzine Y3 (3), (+)-huperzine Z (4a) and (-)-huperzine Z (4b) as well as ten known alkaloids (5-14) were isolated from Huperzia serrata. The structures of the new compounds were established using extensive spectroscopic (1D and 2D NMR, IR, and HRESIMS) and calculated electronic circular dichroism (ECD) methods. Compounds 4a and 4b were a pair of enantiomers successfully separated by chiral HPLC resolution. Compounds 2 and 3 indicated inhibitory activities against acetylcholinesterase with IC50 value of 57.1 ± 1.6 and 32.7 &p...
Source: Fitoterapia - July 24, 2019 Category: Biochemistry Authors: Jiang F, Qi B, Ding N, Yang H, Jia F, Luo Y, Wang J, Liu X, Wang X, Tu P, Shi S Tags: Fitoterapia Source Type: research
Two new alkaloids isolated from traditional Chinese medicine Binglang the fruit of Areca catechu.
Abstract Binglang, the fruit of Areca catechu L, has a long history as an important Chinese herbal medicine. Two new alkaloids (1 and 2), along with forty-one known compounds (3-43) were isolated from the dried fruit of Areca catechu L. The structures were elucidated on basis of the IR, UV, MS and 1D, 2D NMR spectroscopic data. Compounds 26 and 33 showed weak cytotoxicity against human gastric cancer cell line (BGC-823) with IC50 of 15.91 μM and 20.13 μM, respectively. PMID: 31351128 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - July 24, 2019 Category: Biochemistry Authors: Cao M, Yuan H, Daniyal M, Yu H, Xie Q, Liu Y, Li B, Jian Y, Peng C, Tan D, Peng Y, Choudhary MI, Rahman AU, Wang W Tags: Fitoterapia Source Type: research
Lichen butyrolactone derivatives disrupt oral bacterial membrane.
This study revealed that butyrolactones can be considered as potent antibacterial compounds against oral pathogens causing medical complications. PMID: 31344394 [PubMed - as supplied by publisher] (Source: Fitoterapia)
Source: Fitoterapia - July 22, 2019 Category: Biochemistry Authors: Sweidan A, Smida I, Chollet-Krugler M, Sauvager A, Vallet J, Gouault N, Oliviero N, Tamanai-Shacoori Z, Burel A, van de Weghe P, Chokr A, Tomasi S, Bousarghin L Tags: Fitoterapia Source Type: research
Vochysiamides A and B: Two new bioactive carboxamides produced by the new species Diaporthe vochysiae.
Abstract Endophytic have been considered a rich source for bioactive secondary metabolites with novel chemical structures. A high diverse group of endophytes, isolated from different medicinal plants, belongs to the genus Diaporthe. In a previously study performed by our group the crude extract of strain LGMF1583 showed considerable antibacterial activity mainly against Gram-negative bacteria. Based on ITS phylogeny analysis, strain LGMF1583 was identified as belonging to Diaporthe genus and may represent a new species. In the present study, we described the new species Diporthe vochysiae based on multilocus phylo...
Source: Fitoterapia - July 22, 2019 Category: Biochemistry Authors: Noriler SA, Savi DC, Ponomareva LV, Rohr J, Thorson JS, Rodrigues R, Glienke C, Shaaban KA Tags: Fitoterapia Source Type: research
Chemical constituents from the stems of Securidaca inappendiculata Hassk.
Abstract Three new neolignan glycosides, (7R,8S)-4-hydroxy-3,3'-dimethoxy-8,4'-oxyneoligna-7,9,9'-triol-4-O-β-d-glucopyranosyl-(1 → 4)-β-D-glucopyranoside (1), (7R,8S)-4-hydroxy-3,5'-dimethoxy-4',7-epoxy-8,3'-neoligna-9,9'-diol-9'-O-β-d-glucopyranosyl-4-O-[β-d-glucopyranosyl-(1 → 4)]-β-D-glucopyranoside (2), and (7R,8S)-4-hydroxy-3,5,5'-trimethoxy-4',7-epoxy-8,3'-neoligna-9,9'-diol-9'-O-β-d-glucopyranosyl-4-O-[β-d-glucopyranosyl-(1 → 4)]-β-D-glucopyranoside (3), one new phenolic glycoside, securiphenoside B (4) and two new hemiterpene glycosides, s...
Source: Fitoterapia - July 18, 2019 Category: Biochemistry Authors: Ji J, Wang Q, Wang M, Chen J, Li X Tags: Fitoterapia Source Type: research
GIRK channel activity of Hungarian mushrooms: From screening to biologically active metabolites.
losi L, Hohmann J Abstract In the current study effects of fungal extracts on the G-protein-activated inwardly rectifying potassium channel (GIRK1/4) were screened using the automated patch-clamp method. 40 organic (n-hexane, chloroform, and 50% methanol) and aqueous extracts were prepared from 10 mushroom species native to Hungary. Among the examined fungal fractions of different polarities some n-hexane and chloroform extracts exerted considerable ion channel activity. One of the most active fungal species, Hypholoma lateritium was selected for further detailed examination to determine the compounds responsible ...
Source: Fitoterapia - July 18, 2019 Category: Biochemistry Authors: Ványolós A, Orvos P, Chuluunbaatar B, Tálosi L, Hohmann J Tags: Fitoterapia Source Type: research
Antidiarrhoeal mechanism study of fulvic acids based on molecular weight fractionation.
In this study, we used ultrafiltration technique to fractionate this heterogeneous mixture into a series of relatively uniform fractions. The main structural features of FA and its fractions were characterized, and at the same time their antidiarrhoeal activities on drug-induced diarrhoea model mice were evaluated and the collagen content in the intestine of mice were determined. Through contrastive study of the relative variations between structure characteristics and antidiarrhoeal activities with the change of molecular weight, we found that the oxygen-containing functional groups especially phenolic hydroxyl groups, mo...
Source: Fitoterapia - July 18, 2019 Category: Biochemistry Authors: Qin Y, Zhang M, Dai W, Xiang C, Li B, Jia Q Tags: Fitoterapia Source Type: research
The genus Amoora: A phytochemical and pharmacological review.
Abstract The genus Amoora belongs to the Meliaceae family comprising approximately 25-30 species. Many Amoora species have been used as folk medicines for the treatment of many diseases. This review focuses on diverse chemical constituents from Amoora species as well as significant pharmacological activities. Up to now, a total of 140 compounds including eight sesquiterpenoids, 26 diterpenoids, 42 triterpenoids, 22 limonoids, seven steroids, seven alkaloids, seven rocaglamide derivatives, four flavonoids, four glycosides, two coumarins, nine phenols, and two organic acids and esters were reported from Amoora speci...
Source: Fitoterapia - July 16, 2019 Category: Biochemistry Authors: Xu WH, Su XM, Wang C, Du F, Liang Q Tags: Fitoterapia Source Type: research