Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides.
Authors: Mihăşan M, Wormwood KL, Sokolowska I, Roy U, Woods AG, Darie CC
Abstract
Recent developments of mass spectrometry (MS) allow us to identify, estimate, and characterize proteins and protein complexes. At the same time, structural biology helps to determine the protein structure and its structure-function relationship. Together, they aid to understand the protein structure, property, function, protein-complex assembly, protein-protein interaction, and dynamics. The present chapter is organized with illustrative results to demonstrate how experimental mass spectrometry can be combined with computat...
Source: Advances in Experimental Medicine and Biology - July 29, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research
Identification of Posttranslational Modifications (PTMs) of Proteins by Mass Spectrometry.
Identification of Posttranslational Modifications (PTMs) of Proteins by Mass Spectrometry.
Adv Exp Med Biol. 2019;1140:199-224
Authors: Aslebagh R, Wormwood KL, Channaveerappa D, Wetie AGN, Woods AG, Darie CC
Abstract
There are only 30,000 human genes, which, according to the central dogma from biology, it means that there should be 30,000 mRNA and 30,000 proteins. However, there are at least 1-2 million protein entities that are expressed in a cell at a given time. This is primarily due to alternative splicing in different cells and tissues, which may lead to expression of diffe...
Source: Advances in Experimental Medicine and Biology - July 29, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research
Mass Spectrometry for Proteomics-Based Investigation.
Authors: Woods AG, Sokolowska I, Ngounou Wetie AG, Channaveerappa D, Dupree EJ, Jayathirtha M, Aslebagh R, Wormwood KL, Darie CC
Abstract
Within the past years, we have witnessed a great improvement is mass spectrometry (MS) and proteomics approaches in terms of instrumentation, protein fractionation, and bioinformatics. With the current technology, protein identification alone is no longer sufficient. Both scientists and clinicians want not only to identify the proteins, but also to identify the protein's post-translational modifications (PTMs), protein isoforms, protein truncation, protein-protein intera...
Source: Advances in Experimental Medicine and Biology - July 29, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research
Ethnobotanical Study of Cultivated Plants in Kai šiadorys District, Lithuania: Possible Trends for New Herbal Based Medicines.
Conclusions: Only 19 of the cultivated medicinal plant species mentioned by interviewed persons are described in the World Health Organization monographs. This means that the remaining 52 species are used without the World Health Organization approved medical indications, based solely on the folk medicine knowledge and experience. This study showed that the folk use of plants is strongly rooted in daily practice in the studied area.
PMID: 31239859 [PubMed] (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - June 27, 2019 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Principles of Allergen Immunotherapy and Its Clinical Application in China: Contrasts and Comparisons with the USA
AbstractAllergen immunotherapy (AIT) for allergic rhinitis (AR), asthma, and other allergic diseases has developed quickly. House dust mite (HDM),Artemisia (wormwood),Humulus japonicus (Japanese hop),Alternaria alternata, andCladosporium herbarum are the five most common inhalant allergens in China. AIT has been performed in China for over 60 years. With the support of the Chinese Medical Association (CMA) and the Chinese Medical Doctors Association (CMDA), the Chinese College of Allergy and Asthma (CCAA) was established in 2016 as a specialized branch of CDMA and is the main certification authority for AIT. Chinese alle...
Source: Clinical Reviews in Allergy and Immunology - June 25, 2019 Category: Allergy & Immunology Source Type: research
Development of a PCR High-Resolution Melt Assay for Artemisia absinthium (Wormwood) and a Triplex Assay with Two Additional "Unregulated Legal High" Species Datura stramonium (Jimson Weed) and Merremia tuberosa (Hawaiian Woodrose).
This study developed a polymerase chain reaction (PCR) high-resolution melt (HRM) assay to detect and identify A. absinthium based on primer specificity, sensitivity, repeatability, and robustness. A triplex assay was performed with three "unregulated legal high" species: Datura stramonium, Merremia tuberosa, and A. absinthium; the PCR HRM assay detected and identified each plant at melt temperatures 77.42 ± 0.20°C, 83.88 ± 0.22°C, and 87.77 ± 0.15°C, respectively. The primer set developed distinguished A. absinthium from a variety of plant species and was successfully triplexed.
PMID: 31157922 [PubM...
Source: Journal of Forensic Sciences - June 2, 2019 Category: Forensic Medicine Authors: Kiesel BD, Elkins KM Tags: J Forensic Sci Source Type: research
Intervention of artemisinin in macular edema associated with retinal vein occlusion: A protocol for a systematic review and meta-analysis
Background:
Artemisinin was discovered to be highly effective antimalarial drugs shortly after the isolation of the parent artemisinin in 1971 in China. It is derived from extracts of sweet wormwood (Artemisia annua) and are well established for the treatment of malaria. Recently, artemisinin has been shown that it might have therapeutic value for several other diseases. The purpose of this review is to assess the efficacy of artemisinin as a treatment for macular edema associated with retinal vein occlusion.
Methods and analysis:
A systematic literature search will be performed in all available databases to quantitat...
Source: Medicine - June 1, 2019 Category: Internal Medicine Tags: Research Article: Study Protocol Systematic Review Source Type: research
Supercritical anti-solvent fractionation of Artemisia absinthium L. conventional extracts: tracking artemetin and casticin
Publication date: Available online 7 May 2019Source: The Journal of Supercritical FluidsAuthor(s): Elisa Langa, Juan I. Pardo, Carlota Giménez-Rota, Azucena González-Coloma, María J. Hernáiz, Ana M. MainarAbstractIn the current work the Supercritical Anti-solvent Fractionation (SAF) methodology was applied to conventional extracts from Artemisia absinthium L. (wormwood). Artemetin and casticin, two compounds found in wormwood extracts and with high structural similarity, were tracked in all the experiments. A Response Surface Methodology (RSM), based on Central Composite Design (CCD) was used for both the experimental ...
Source: The Journal of Supercritical Fluids - May 8, 2019 Category: Chemistry Source Type: research
Methyl 3-(5-(prop-1-yn-1-yl)thiophen-2-yl)propanoate: A Rare Acetylene Derivative from Artemisia absinthium Root Essential Oil.
Authors: Polina D B, Marko S P, Niko S R
Abstract
Methyl 3-(5-(prop-l-yn-l-yl)thiophen-2-yl)propanoate (1), biosynthetically and structurally related to dehydromatricaria ester, was isolated from the root essential oil of Artemisia absinthium L. (0.7% of the total oil). This is the second record of this compound and the very first one regarding it as an essential-oil constituent. In this paper, we give details regarding its isolation, structural elucidation and gas chromatographic properties (RI on DB-5 MS column: 1694). The NMR-based identification of the compound was corroborated by simulation of its 'H-...
Source: Natural Product Communications - March 30, 2019 Category: Biochemistry Tags: Nat Prod Commun Source Type: research
Molecules, Vol. 24, Pages 913: The Extracts of Artemisia absinthium L. Suppress the Growth of Hepatocellular Carcinoma Cells through Induction of Apoptosis via Endoplasmic Reticulum Stress and Mitochondrial-Dependent Pathway
iu
Xu
Li
Artemisia absinthium L. has pharmaceutical and medicinal effects such as antimicrobial, antiparasitic, hepatoprotective, and antioxidant activities. Here, we prepared A. absinthium ethanol extract (AAEE) and its subfractions including petroleum ether (AAEE-Pe) and ethyl acetate (AAEE-Ea) and investigated their antitumor effect on human hepatoma BEL-7404 cells and mouse hepatoma H22 cells. The cell viability of hepatoma cells was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis, cell cycle, mitochondrial membrane potential (Δψm)...
Source: Molecules - March 4, 2019 Category: Chemistry Authors: Wei Xia Ziyayiding Chen Liu Xu Li Tags: Article Source Type: research
The anti-malarial drug artesunate causes cell cycle arrest and apoptosis of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells.
In this study, we demonstrate a dose- and time-dependent inhibitory effect of ART on the growth of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells, which was the result of both anti-proliferative and cytotoxic activities. ART inhibited breast cancer cell proliferation via a reactive oxygen species (ROS)-dependent G2/M arrest and ROS-independent G1 arrest. ART-treated MDA-MB-468 and SK-BR-3 cells also experienced apoptotic cell death, which was both ROS- and iron-dependent. ART-induced oxidative stress caused the loss of mitochondrial outer membrane integrity and damage to the cellular DNA of MDA-MB...
Source: Experimental and Molecular Pathology - January 17, 2019 Category: Pathology Authors: Greenshields AL, Fernando W, Hoskin DW Tags: Exp Mol Pathol Source Type: research
The Use of Traditional and Complementary Medicine for Diabetes in Rural Guatemala.
CONCLUSION: There is widespread use of medicinal plants in San Lucas Tolimán. More research is needed to understand the degree of glycemic control in these communities.
PMID: 30449743 [PubMed - in process] (Source: Journal of Health Care for the Poor and Underserved)
Source: Journal of Health Care for the Poor and Underserved - November 21, 2018 Category: International Medicine & Public Health Authors: Andrews CM, Wyne K, Svenson JE Tags: J Health Care Poor Underserved Source Type: research
An insight into chemical composition and bioactivity of 'Prokupac' red wine.
Abstract
Autochthonous variety 'Prokupac' (Vitis vinifera L.) red wines with the addition of selected aromatic herbs (Artemisia absinthium, Cinnamomum zeylanicum, Glycyrrhiza glabra and Pimpinella anisum L.) were evaluated for chemical composition and biological activity. Furthermore, their polyphenolic profiles were determined by HPLC-DAD. While total phenolic (TPC) and flavonoid (TFC) contents, along with antioxidant activity, were significantly higher in all examined wine samples, the content of total anthocyanins was decreased, compared to a pure 'Prokupac' wine, used as the control one. On the other h...
Source: Natural Product Research - November 16, 2018 Category: Biochemistry Authors: Lakićević SH, Popović Djordjević JB, Pejin B, Djordjević AS, Matijašević SM, Lazić ML Tags: Nat Prod Res Source Type: research
Ovicidal and larvicidal activity of extracts from medicinal-plants against Haemonchus contortus.
Abstract
The use of medicinal plants (MP) containing bioactive compounds is an alternative strategy to control of parasitic nematode of small ruminants Haemonchus contortus at various stages of their life cycle. The aims of this study were to determine the in vitro anthelmintic activity of both aqueous and methanolic extracts from 13 medicinal plants typical for Central Europe, and to determine quantity of selected plant secondary metabolites (PSMs) in the methanolic extracts. In vitro egg hatch test and larval development tests were conducted to determine the possible anthelmintic effects of methanolic an...
Source: Experimental Parasitology - October 30, 2018 Category: Parasitology Authors: Váradyová Z, Pisarčíková J, Babják M, Hodges A, Mravčáková D, Kišidayová S, Königová A, Vadlejch J, Várady M Tags: Exp Parasitol Source Type: research
Effect of Artemisia annua and Artemisia afra tea infusions on schistosomiasis in a large clinical trial.
ConclusionBoth A. annua and A. afra provided faster effective treatment of schistosomiasis and should be considered for implementation on a global scale.Graphical abstract (Source: Phytomedicine)
Source: Phytomedicine - October 12, 2018 Category: Drugs & Pharmacology Source Type: research
