The critical roles of lncRNAs in the pathogenesis of melanoma.
Abstract Long non-coding RNAs (lncRNAs) embrace a huge fraction of human transcripts and participate in the pathogenesis of human disorders especially malignant conditions. Malignant melanoma, as the most fatal type of cutaneous malignnacies, is associated with dysregulation of several lncRNAs including PVT1, H19, MALAT1, and CCAT1. Moreover, a portion of lncRNAs are exclusively expressed in melanoma cell lines. Expression levels of several lncRNAs are associated with TNM stage, tumor size and progression of melanoma. Thus, these lncRNAs are regarded as biomarkers for this malignancy. Peripheral transcript levels ...
Source: Experimental and Molecular Pathology - October 20, 2020 Category: Pathology Authors: Safa A, Gholipour M, Dinger ME, Taheri M, Ghafouri-Fard S Tags: Exp Mol Pathol Source Type: research

Circ-XPO1 upregulates XPO1 expression by sponging multiple miRNAs to facilitate osteosarcoma cell progression.
Abstract Circular RNAs (circRNAs) act as a key role in mediating carcinogenesis. Nevertheless, the functions and mechanisms of circRNAs in osteosarcoma (OS) are still not fully understood. In the present study, we aim to investigate the functions of circ-XPO1 in OS and its potential mechanism underlying OS progression. CircRNA microarray indicated elevation of circ-XPO1 in OS specimens relative to normal samples. Elevation of circ-XPO1 and XPO1 mRNA was identified in OS tissue specimens and cells by qRT-PCR. In addition, enhanced expression of circ-XPO1 and XPO1 mRNA both correlated with poor prognosis for the pat...
Source: Experimental and Molecular Pathology - October 19, 2020 Category: Pathology Authors: Jiang Y, Hou J, Zhang X, Xu G, Wang Y, Shen L, Wu Y, Li Y, Yao L Tags: Exp Mol Pathol Source Type: research

Increased expression of ALDH-1 is associated with clinical parameters of salivary glands neoplasms.
Abstract The enzyme aldehyde dehydrogenase-1 (ALDH-1) is a known putative tumour stem cells (TSC) marker, and these cells are implicated in carcinogenesis and progression of human neoplasms. We aimed to evaluate ALDH-1 expression in benign and malignant salivary gland neoplasms and its clinicopathological and prognostic significance. Expression of ALDH-1 was investigated by immunohistochemistry and confirmed by Western Blot analysis in 154 salivary gland neoplasms (103 malignant and 51 benign neoplasms). The expression was identified in the parenchyma of malignant (n = 88; 85.6%) and benign (100%) neoplasms. O...
Source: Experimental and Molecular Pathology - October 11, 2020 Category: Pathology Authors: da Silva LP, de Sousa Lopes MLD, Sarmento ASC, de Albuquerque Borges M, de Moura SRS, Sobral APV, de Souza LB Tags: Exp Mol Pathol Source Type: research

miRNA signature in glioblastoma: Potential biomarkers and therapeutic targets.
Abstract MicroRNAs (miRNAs) are transcripts with sizes of about 22 nucleotides, which are produced through a multistep process in the nucleus and cytoplasm. These transcripts modulate the expression of their target genes through binding with certain target regions, particularly 3' suntranslated regions. They are involved in the pathogenesis of several kinds of cancers, such as glioblastoma. Several miRNAs, including miR-10b, miR-21, miR-17-92-cluster, and miR-93, have been up-regulated in glioblastoma cell lines and clinical samples. On the other hand, expression of miR-7, miR-29b, miR-32, miR-34, miR-181 family m...
Source: Experimental and Molecular Pathology - September 29, 2020 Category: Pathology Authors: Rezaei O, Honarmand K, Nateghinia S, Taheri M, Ghafouri-Fard S Tags: Exp Mol Pathol Source Type: research

Angiotensin inhibition and cellular senescence in the developing rat kidney.
In this study, we aimed to investigate the effect of renin angiotensin system (RAS) blockade on renal cell senescence in the developing rat kidney. Newborn rat pups were treated with enalapril (30 mg/kg/day) or vehicle for seven days after birth. We investigated the intrarenal expressions of cell cycle regulators p21 and p16 with immunoblots and immunohistochemistry at postnatal day 8. For the determination of renal cellular senescence, immunostaining for senescence-associated β-galactosidase (SA-β-gal) and telomerase reverse transcriptase (TERT) was also performed. Enalapril treatment showed significant altera...
Source: Experimental and Molecular Pathology - September 29, 2020 Category: Pathology Authors: Yoo KH, Yim HE, Bae ES Tags: Exp Mol Pathol Source Type: research

Human papillomavirus-positivity is associated with EREG down-regulation and promoter hypermethylation in head and neck squamous cell carcinoma.
CONCLUSION: We conclude that EREG is the target of epigenetic regulation in HPV+ HNCs and its suppressed expression through promoter hypermethylation is associated with the development of HPV-associated HNCs. PMID: 33007298 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 28, 2020 Category: Pathology Authors: Khanal S, Strickley JD, Ha T, Demehri S, Ghim SJ, Jenson AB, Redman RA, Joh JJ Tags: Exp Mol Pathol Source Type: research

High prevalence of ROS1 gene rearrangement detected by FISH in EGFR and ALK negative lung adenocarcinoma.
Abstract ROS1 rearrangement has become an important biomarker for targeted therapy in advanced lung adenocarcinoma (LUAD). The study aimed to evaluate the prevalence of ROS1 rearrangement in Chinese LUAD with EGFR wild-type and ALK fusion-negative status, and analyze the relationship with their clinicopathological characteristics. A large cohort of 589 patients of LUAD with EGFR/ALK wild-type, diagnosed between April 2014 and June 2018, was retrospectively analyzed. ROS1 rearrangement in all these cases was detected by FISH, and 8 selected cases with different positive and negative signals were confirmed by NGS. A...
Source: Experimental and Molecular Pathology - September 22, 2020 Category: Pathology Authors: Xu Y, Chang H, Wu L, Zhang X, Zhang L, Zhang J, Li Y, Shen L, Zhu X, Zhou X, Bai Q Tags: Exp Mol Pathol Source Type: research

Expression profiling revealed up-regulation of three lncRNAs in breast cancer samples.
Abstract Long non-coding RNAs (lncRNAs) have been vastly investigated for their critical roles in the pathogenesis of breast cancer. Yet, the expression pattern and clinical significance of three lncRNAs namely CTBP1AS2, LINC-ROR and SPRY4-IT1 in breast cancer are not completely clarified. In the present investigation, we assessed expression of these lncRNAs in breast cancer tissues and paired non-cancerous specimens from the same patients using quantitative real time PCR. Notably, expression of CTBP1AS2, LINC-ROR and SPRY4-IT1 were upregulated in breast cancer tissues compared with non-cancerous tissues (ER = 17.62, P value
Source: Experimental and Molecular Pathology - September 21, 2020 Category: Pathology Authors: Mohebi M, Sattari A, Ghafouri-Fard S, Modarressi MH, Kholghi-Oskooei V, Taheri M Tags: Exp Mol Pathol Source Type: research

Neuro-protective roles of long non-coding RNA MALAT1 in Alzheimer's disease with the involvement of the microRNA-30b/CNR1 network and the following PI3K/AKT activation.
This study aimed to probe the function of lncRNA MALAT1 in neuronal recovery in Alzheimer's disease (AD). Aβ25-35 was used induce AD in a rat model and neuronal injury in PC12 and C6 cells. Aberrantly expressed lncRNAs/microRNAs (miRNAs) in AD rats were screened out by microarray analyses. Altered expression of MALAT1, miR-30b and CNR1 was performed to explore their roles in neuronal recovery in rat and cell models. Consequently, LncRNA MALAT1 and CNR1 were poorly expressed while miR-30b was highly expressed in Aβ25-35-induced rat models and cells. Overexpression of MALAT1 or CNR1 reduced neuronal injury in rat h...
Source: Experimental and Molecular Pathology - September 21, 2020 Category: Pathology Authors: Li L, Xu Y, Zhao M, Gao Z Tags: Exp Mol Pathol Source Type: research

Role of RBM3 in the regulation of cell proliferation in hepatocellular carcinoma.
Abstract RNA binding motif protein 3 (RBM3) has been shown to be upregulated in several types of human tumors. Using tissue microarrays and immunohistochemistry, we showed here that both nuclear and cytoplasmic RBM3 expression levels were higher in hepatocellular carcinoma (HCC) tissues than in adjacent non-tumorous tissues. High nuclear RBM3 was found to be correlated with larger tumor size (P = 0.030), high serum AFP levels (P = 0.011), and advanced Edmonson grading (P = 0.006). Cytoplasmic RBM3 was associated with advanced Edmonson grading (P = 0.003). Kaplan-Meier survival analysis revealed tha...
Source: Experimental and Molecular Pathology - September 21, 2020 Category: Pathology Authors: Miao X, Zhang N Tags: Exp Mol Pathol Source Type: research

Long non-coding RNA Hsp4 alleviates lipopolysaccharide-induced apoptosis of lung epithelial cells via miRNA-466m-3p/DNAjb6 axis.
This study investigated the effects of LncRNA Hsp4 (Hsp4, ENSMUST00000175718) on lipopolysaccharide (LPS)-induced apoptosis of MLE-12 cells. In our research, we found that LPS treatment remarkably induced apoptosis of MLE-12 cells and decreased the expression of Hsp4. Overexpression of Hsp4 significantly reversed LPS-induced cell apoptosis through inhibiting mTOR signaling, while suppression of Hsp4 presented opposite effects. Further results showed that Hsp4 positively regulated the expression of miR-466m-3p. Knockdown of miR-466m-3p reversed LPS-induced cell apoptosis via increasing the levels of DNAjb6 which was confirm...
Source: Experimental and Molecular Pathology - September 21, 2020 Category: Pathology Authors: Ji Q, Pan C, Wang J, Yang Z, Li C, Yang C, Zhang W, Wang M, Dong M, Sun Z, Nie S Tags: Exp Mol Pathol Source Type: research

Long noncoding RNA PTCSC1 drives esophageal squamous cell carcinoma progression through activating Akt signaling.
Abstract Long noncoding RNAs (lncRNAs) have critical roles in various malignancies. However, the specific expression and roles of lncRNA PTCSC1 in esophageal squamous cell carcinoma (ESCC) are still unknown. Here, we identified that lncRNA PTCSC1 was elevated in ESCC tissues and cell lines compared with adjacent noncancerous tissues and normal esophageal epithelial cell line, respectively. Enhanced expression of PTCSC1 facilitated ESCC cells proliferation and migration in vitro and ESCC xenograft growth in vivo. Conversely, deficiency of PTCSC1 suppressed ESCC cells proliferation and migration in vitro and ESCC tu...
Source: Experimental and Molecular Pathology - September 20, 2020 Category: Pathology Authors: Liu T, Liang X, Yang S, Sun Y Tags: Exp Mol Pathol Source Type: research

SOX11-induced decrease in vimentin and an increase in prostate cancer cell migration attributed to cofilin activity.
Abstract SOX11 is a transcription factor in the SOX family of genes that regulate multiple cellular events by influencing the expression of key genes in developmental, physiological, and tumorigenic cells. To elucidate the role of SOX11 in prostate cancer cells, PC-3 prostate cancer cells were cloned (S6 and S9 cells) to highly express SOX11. We demonstrated that both S6 and S9 lose vimentin expression, acquiring epithelial marker proteins, which indicates the Epithelial state phenotype. S6 and S9 cells have cancer-promoting characteristics that include higher migratory properties compared with control cells. The ...
Source: Experimental and Molecular Pathology - September 20, 2020 Category: Pathology Authors: Hirokawa YS, Kanayama K, Kagaya M, Shimojo N, Uchida K, Imai H, Ishii K, Watanabe M Tags: Exp Mol Pathol Source Type: research

Alternations in mitochondrial genome in carcinogenesis of HPV positive cervix.
CONCLUSIONS: We hypothesize that mutations in MT-ND1 observed in H-SIL and cancer could activate cervical carcinogenesis by increased ROS production. PMID: 32931837 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 11, 2020 Category: Pathology Authors: Warowicka A, Wołuń-Cholewa M, Kwaśniewska A, Goździcka-Józefiak A Tags: Exp Mol Pathol Source Type: research

Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P.
In this study, the expression of GAS5 and miR-223-3p in peripheral blood of CHF patients and healthy subjects was first detected, GAS5 was low in CHF while miR-223-3p was high (P 
Source: Experimental and Molecular Pathology - September 10, 2020 Category: Pathology Authors: Li G, Du P, Qiang X, Jin D, Liu H, Li B, Guo J Tags: Exp Mol Pathol Source Type: research

Dysregulation of non-coding RNAs in autoimmune thyroid disease.
Abstract Autoimmune thyroid disease (AITD) is a complex disorder with both genetic and environmental risk factors. A number of genetic factors such as HLA and CTLA-4 loci have been associated with risk of this disorder. In addition to these factors, recent studies have shown contribution of non-coding RNAs in the pathogenesis of this condition. Several microRNAs (miRNAs) and a number of long noncoding RNAs (lncRNAs) such as IFNG-AS1, Heg, NR_038461, NR_038462, T204821 and NR_104125 have been dysregulated in peripheral blood of patients with AITD. These transcripts have been mostly enriched in pathways that modulat...
Source: Experimental and Molecular Pathology - September 7, 2020 Category: Pathology Authors: Taheri M, Eghtedarian R, Dinger ME, Ghafouri-Fard S Tags: Exp Mol Pathol Source Type: research

Investigating the regulatory function of the ANO1-AS2 on the ANO1 gene in infertile men with asthenozoospermia and terato-asthenozoospermia.
Abstract Long non-coding RNAs (lncRNAs) have a particular expression in the testicular tissue and exhibit a regulatory function on the reproduction system. ANO1-AS2 (linc02584), as an lncRNA is located near the anoctamin1 (ANO1) gene. ANO1 is an important component of the transmembrane system exhibiting expression modifications in the idiopathic infertile men. Therefore, the present study was conducted to investigate the relationship between ANO1-AS2 and ANO1 gene expression with sperm motility and morphology in the patients with asthenozoospermia (AZ) and terato- asthenozoospermia (TAZ). The study population incl...
Source: Experimental and Molecular Pathology - September 7, 2020 Category: Pathology Authors: Saberiyan M, Mirfakhraie R, Gholami D, Dehdehi L, Teimori H Tags: Exp Mol Pathol Source Type: research

Nucleotide-binding oligomerization domain protein 1 enhances oxygen-glucose deprivation and reperfusion injury in cortical neurons via activation of endoplasmic reticulum stress-mediated autophagy.
This study provides new insights for the target of CIR injury treatment. PMID: 32888957 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 31, 2020 Category: Pathology Authors: Ma X, Zhang W, Xu C, Zhang S, Zhao J, Pan Q, Wang Z Tags: Exp Mol Pathol Source Type: research

Differential mobility spectrometry imaging for pathological applications.
In this study, we show that differential ion mobility spectrometry (DMS) is a viable option in tissue imaging. We demonstrate that a DMS-driven solution performs with up to 92% accuracy in differentiating between two grossly distinct animal tissues. In addition, our model is able to classify the correct tissue with 81% accuracy in an eight-class setting. The DMS-based system is a significant innovation in a field dominated by mass-spectrometry-based solutions. By developing the presented platform further, DMS technology could be a cost-effective and helpful tool for automated pathological analysis. PMID: 32888958 [Pub...
Source: Experimental and Molecular Pathology - August 31, 2020 Category: Pathology Authors: Kontunen A, Tuominen J, Karjalainen M, Anttalainen O, Tolonen T, Kumpulainen P, Lepomäki M, Vehkaoja A, Oksala N, Roine A Tags: Exp Mol Pathol Source Type: research

The relationship between oxidative stress and cytogenetic abnormalities in B-cell chronic lymphocytic leukemia.
CONCLUSION: The results indicate that B-CLL patients experience increased oxidative stress and the relative deficiency of the antioxidant defense system. Increased CD level was independently associated with greater likelihood of harboring CA. PMID: 32882207 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 30, 2020 Category: Pathology Authors: Zhevak T, Shelekhova T, Chesnokova N, Tsareva O, Chanturidze A, Litvitsky P, Andriutsa N, Samburova N, Budnik I Tags: Exp Mol Pathol Source Type: research

MiR-302a-3p aggravates myocardial ischemia-reperfusion injury by suppressing mitophagy via targeting FOXO3.
CONCLUSION: Our study suggested that inhibition of miR-302a-3p promoted mitochondrial autophagy and inhibited oxidative stress by targeting FOXO3 to suppress myocardial apoptosis, representing a potential target for MIRI treatment. PMID: 32866521 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 27, 2020 Category: Pathology Authors: Lv W, Jiang J, Li Y, Fu L, Meng F, Li J Tags: Exp Mol Pathol Source Type: research

Important role of annexin A2 (ANXA2) in new blood vessel development in vivo and human triple negative breast cancer (TNBC) growth.
In this report, we used Matrigel plug model of in vivo angiogenesis and confirmed its role in new blood vessel development. Next, we tested if blocking of ANX A2 in aggressive human breast TME can inhibit angiogenesis and tumor growth in vivo. We showed that aggressive human breast tumor cells growing in nude mice can induce intense neoangiogenesis in the tumor mass. Blocking of ANXA2 significantly inhibited neoangiogenesis and resulted in inhibition of tumor growth. Interestingly, we identified that blocking of ANXA2 significantly inhibited tyrosine phosphorylation (Tyr-P) of ANXA2 implying its involvement in tyrosine sig...
Source: Experimental and Molecular Pathology - August 27, 2020 Category: Pathology Authors: Sharma MC, Jain D Tags: Exp Mol Pathol Source Type: research

MicroRNA-29a functions as a tumor suppressor through targeting STAT3 in laryngeal squamous cell carcinoma.
In this study, we investigated the tumor suppressive role of miRNA-29a in laryngeal squamous cell carcinoma. We performed cell-based functional analysis after overexpression of miR-29a in AMC-HN-8 and HEP2 cells in vitro. It turned out that ectopic expression of miR-29a significantly inhibited cell proliferation, invasion, and migration in two LSCC cell lines (AMC-HN-8 and Hep2) in vitro. Further downstream target analysis by western blotting and luciferease reporter assay showed that overexpression of miR-29a significantly downregulated expression of STAT3, and STAT3 is a putative direct target of miR-29a in LSCC. In addi...
Source: Experimental and Molecular Pathology - August 24, 2020 Category: Pathology Authors: Liu YB, Wang Y, Zhang MD, Yue W, Sun CN Tags: Exp Mol Pathol Source Type: research

Laser capture microdissection on formalin-fixed and paraffin-embedded renal transplanted biopsies: Technical perspectives for clinical practice application.
Abstract Renal biopsy (RBx) is an essential tool in the diagnostic and therapeutic process of most native kidney diseases and in the renal transplanted graft. Laser capture microdissection (LCM), combined with molecular biology, might improve the diagnostic power of RBx. However, the limited amount of available renal tissue is often an obstacle for achieving a satisfactory qualitative and quantitative analysis. In our work we present a method which allows us to obtain good quality and quantity of RNA from formalin-fixed and paraffin-embedded (FFPE) renal tissue derived from RBx performed in transplanted patients. ...
Source: Experimental and Molecular Pathology - August 23, 2020 Category: Pathology Authors: Alfieri CM, Mattinzoli D, Ikehata M, Cresseri D, Moroni G, Valentina V, Ferri G, Ferrero S, Messa P Tags: Exp Mol Pathol Source Type: research

Central nervous system (CNS) tumor cell heterogeneity contributes to differential platinum-based response in an in vitro 2D and 3D cell culture approach.
, Silva LM Abstract One of the models that best explains the cellular heterogeneity observed in central nervous system (CNS) tumors is the presence of cancer stem cells (CSCs). CSCs can originate from differentiated adult cells that return to an undifferentiated stage through the mechanism known as epithelial-mesenchymal transition (EMT). In this paper, we evaluated cellular and molecular heterogeneity and the participation of the epithelial-mesenchymal transition (EMT) in glioblastoma (U-87 MG and LN-18) and neuroblastoma (KELLY and IMR-32) cell lines cultured in monolayer (2D) and neurosphere (CSC enrichment- 3D...
Source: Experimental and Molecular Pathology - August 19, 2020 Category: Pathology Authors: Gonçalves BÔP, Fialho SL, Silvestrini BR, Sena IFG, Dos Santos GSP, de Assis Gomes D, Silva LM Tags: Exp Mol Pathol Source Type: research

Novel Biocartis Idylla ™ cartridge-based assay for detection of microsatellite instability in colorectal Cancer tissues.
CONCLUSION: The novel Biocartis Idylla™ cartridge-based MSI assay showed complete concordance with MMR IHC status and Promega MSI assay. These findings support the use of the Biocartis Idylla™ cartridge-based MSI assay as a rapid and cost-effective alternative method to determine MMR status. PMID: 32822722 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 17, 2020 Category: Pathology Authors: Mindiola-RomeroMD AE, GreenBS DC, Al-TurkmaniPhD MR, GodwinBS KN, MackayBS AC, TafeMD LJ, RenMD B, TsongalisPhD GJ Tags: Exp Mol Pathol Source Type: research

Long noncoding RNA-GAS5 retards renal fibrosis through repressing miR-21 activity.
CONCLUSION: Activation of lncRNA-GAS5 attenuates kidney fibrosis by modulating miR-21 activity and may serve as a surrogate biomarker in monitoring CKD progression. PMID: 32791063 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 9, 2020 Category: Pathology Authors: Yu Y, Jiang H, Niu Y, Huang J, Zhang X, Liu X, Zhang Y, Liu S, Fu H, Yu C Tags: Exp Mol Pathol Source Type: research

Well-differentiated liposarcoma primary from thymic stroma: Case report and literature review.
We describe the case of a young female diagnosed with a well-differentiated lipomatous tumor primary of thymic stroma with near cut-off result for MDM2-FISH, but positive immunohistochemical staining for the panel described above. We discuss the challenges in the diagnosis of this rare entity and a present a literature review. PMID: 32791064 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - August 9, 2020 Category: Pathology Authors: Furlan K, Miller I, Rohra P, Mir F, Ocampo Gonzales FA, Gattuso P Tags: Exp Mol Pathol Source Type: research

miR-188-5p inhibits apoptosis of neuronal cells during oxygen-glucose deprivation (OGD)-induced stroke by suppressing PTEN.
Abstract The miRNAs and mRNAs are found to play a crucial role in modulating different diseases including stroke, according to the recent evidence. The current study is aimed at assessing the functional role played by miR-188-5p in the regulation of cell apoptosis and viability in OGD-induced human neural cell line HNC. With the help of RT-qPCR, the authors determined miR-188-5p as well as its putative target PTEN among OGD-treated cells in different treatment times. The cell viability was assessed through CCK-8 assay while the cell transfection either upregulated or may have silenced the genes. Both Western Blot ...
Source: Experimental and Molecular Pathology - July 30, 2020 Category: Pathology Authors: Li L, Cui P, Ge H, Shi Y, Wu X, Ruan Z Tags: Exp Mol Pathol Source Type: research

Clinical implementation of plasma EGFR T790M testing using droplet digital PCR in TKI-resistant NSCLC patients.
This study investigated the positive rate, specimen type for rebiopsy and clinical impact of blood-based EGFR T790M testing. METHODS: We retrospectively evaluated clinical samples that underwent plasma EGFR T790M testing in TTSH Molecular Diagnostic Laboratory from August 2017 to September 2019. Data on diagnosis, EGFR activating and T790M mutations, and treatment strategies were recorded. RESULTS: A total of 104 progressive NSCLC cases were included in this study. Overall, 46 patients (44.2%) were tested T790M positive, and 47.8% of these tested positive had low levels (defined as ≤3% fractional abundance and 
Source: Experimental and Molecular Pathology - July 28, 2020 Category: Pathology Authors: Chan DLH, Toh GLX, Goh LL Tags: Exp Mol Pathol Source Type: research

Predictive and prognostic value of LSP1 rs3817198 in sporadic breast cancer in northeastern population of Iran.
PMID: 32738313 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 28, 2020 Category: Pathology Authors: Afzaljavan F, Moezzi A, Vhednia E, Shamshiri AK, Vakili F, Shandiz FH, Pasdar A Tags: Exp Mol Pathol Source Type: research

Prognostic value of tumor stromal collagen features in patients with hepatocellular carcinoma revealed by second-harmonic generation microscopy.
CONCLUSION: Our results indicate the prognostic value of the quantitative evaluation of tumor stromal collagen using second harmonic generation imaging of patients with HCC. PMID: 32735795 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 27, 2020 Category: Pathology Authors: Lin L, Chen G, Chen Z, Lu J, Zhu W, Zhong J, Peng F, Huang A Tags: Exp Mol Pathol Source Type: research

The role of genetic polymorphism within PD-L1 gene in cancer. Review.
Abstract The maintenance of homeostasis of the immune system depends on the balance between excitatory and inhibitory signals. Programmed death ligand (PD-L1) is a molecule which downregulates the immune system targeting the programmed death receptor 1 (PD-1). Under physiological conditions, the receptor is constitutively expressed in lymphocytes. The PD-L1 / PD-1 pathway plays a key role in completing the immune response in the right way, preventing excessive stimulation of the cells of the immune system, protecting the organism against autoimmunity. Under pathological conditions PD-L1 expression may take place i...
Source: Experimental and Molecular Pathology - July 13, 2020 Category: Pathology Authors: Kula A, Dawidowicz M, Kiczmer P, Seńkowska AP, Świętochowska E Tags: Exp Mol Pathol Source Type: research

Circ_DCAF6 potentiates cell stemness and growth in breast cancer through GLI1-Hedgehog pathway.
Abstract Circular RNAs (circRNAs) are extensively revealed as a malignant activator or suppressor in multiple pathological processes including cancer cell stemness and growth. However, the association of circ_DCAF6 with breast cancer (BC) cell growth and stemness has not been well depicted. In this research, qRT-PCR clarified the high level of circ_DCAF6 in BC cells. In functional aspects, BC cells presented suppressed proliferation and stemness in the absence of circ_DCAF6. The potential correlation of circ_DCAF6 with Hedgehog (Hh) pathway was unveiled utilizing its specific inhibitor or agonist in qRT-PCR and fu...
Source: Experimental and Molecular Pathology - July 11, 2020 Category: Pathology Authors: Ye G, Pan R, Zhu L, Zhou D Tags: Exp Mol Pathol Source Type: research

DICER-AS1 lncRNA: A putative culprit in the pathogenesis of gastric cancer.
Abstract The nuclear factor-κB (NF-κB) has a pivotal role in the pathogenesis of several cancers including gastric cancer. We have recently reported dysregulation of a number of NF-κB-associated lncRNAs in a variety of human disorders including breast cancer and coronary artery disease. In the current study, we evaluated expression of five NF-κB-associated lncRNAs (CHAST, ADINR, DICER1-AS1, HNF1A-AS1 and NKILA) and two NF-κB-associated-mRNA coding genes (CEBPA and ATG5) in gastric cancer tissues and their paired non-cancerous tissues using real time PCR method. Expression of DICER-AS1...
Source: Experimental and Molecular Pathology - July 10, 2020 Category: Pathology Authors: Afrough H, Ghafouri-Fard S, Yousefi H, Pakzad P, Oskooei VK, Taheri M Tags: Exp Mol Pathol Source Type: research

Regulatory effect of LncRNA DRAIC/miR-149-5p/NFIB molecular network on autophagy of esophageal cancer cells and its biological behavior.
CONCLUSION: LncRNA DRAIC is relevant to cell biology and autophagy of EC. In the future, DRAIC may be developed as a key gene for EC diagnosis and treatment. PMID: 32659236 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 9, 2020 Category: Pathology Authors: Li F, Zhou X, Chen M, Fan W Tags: Exp Mol Pathol Source Type: research

LEPR hypomethylation was significantly associated with gastric cancer in males.
CONCLUSION: LEPR hypomethylation can be used to predict the risk of GC in males. And it might also have the potential to predict the recurrence in GC patients. PMID: 32659237 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - July 9, 2020 Category: Pathology Authors: Yu H, Pan R, Qi Y, Zheng Z, Li J, Li H, Ying J, Xu M, Duan S Tags: Exp Mol Pathol Source Type: research

MicroRNA-199a-3p inhibits angiogenesis by targeting the VEGF/PI3K/AKT signalling pathway in an in vitro model of diabetic retinopathy.
CONCLUSION: MiR-199a-3p upregulation ameliorated HG-stimulated angiogenesis of hRMECs by modulating the PI3K/AKT pathway through inhibiting VEGF. Although retinal neovascularization in vivo has not been studied, these in vitro findings provide more evidence for the role of miR-199a-3p upregulation against HG-induced angiogenesis. PMID: 32622012 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 30, 2020 Category: Pathology Authors: Wang L, Liu WX, Huang XG Tags: Exp Mol Pathol Source Type: research

LncRNA PCGEM1 enhances metastasis and gastric cancer invasion through targeting of miR-129-5p to regulate P4HA2 expression.
CONCLUSION: These findings found that an over-expression of PCGEM1 in GC acts as a miR-129-5p sponge, leading to higher levels of P4HA2. The PCGEM1/miR-129-5p/P4HA2 axis was confirmed to possess a crucial role in GC metastasis and invasion, suggesting its utility as a potential diagnostic and therapeutic biomarker. PMID: 32622013 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 30, 2020 Category: Pathology Authors: Zhang T, Piao HY, Guo S, Zhao Y, Wang Y, Zheng ZC, Zhang J Tags: Exp Mol Pathol Source Type: research

MiR-487a-3p suppresses the malignant development of pancreatic cancer by targeting SMAD7.
CONCLUSIONS: MiR-487a-3p is downregulated in pancreatic cancer samples, which is linked to metastasis and prognosis in pancreatic cancer. It inhibits the malignant development of pancreatic cancer by negatively regulating SMAD7. PMID: 32622014 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 30, 2020 Category: Pathology Authors: Zhou J, Qie S, Fang H, Xi J Tags: Exp Mol Pathol Source Type: research

Aluminum exposure promotes histopathological and pro-oxidant damage to the prostate and gonads of male and female adult gerbils.
Abstract Since the industrial revolution, all living beings have become susceptible to numerous sources of aluminum (Al) exposure. In addition to causing proven toxicity in many organs and systems, Al can also have estrogenic activity when absorbed by the body. The reproductive organs are commonly affected by environmental pollutants with estrogenic activity, but little is known about the effects of Al on the prostate and gonads. Therefore, the aim of this study was to evaluate the effects of subchronic Al exposure on the prostate and gonads of male and female adult gerbils. After 30 days of oral exposure to alu...
Source: Experimental and Molecular Pathology - June 21, 2020 Category: Pathology Authors: da Silva Lima D, da Silva Gomes L, de Sousa Figueredo E, de Godoi MM, Silva EM, da Silva Neri HF, Taboga SR, Biancardi MF, Ghedini PC, Dos Santos FCA Tags: Exp Mol Pathol Source Type: research

Role of different Th17 and Treg downstream signalling pathways in the pathogenesis of Staphylococcus aureus infection induced septic arthritis in mice.
In conclusion it was hypothesized that bone resorption is related with downstream signalling pathways of Th17 cells, which stimulate osteoclast generation via NFκB/JNK-RANKL axis and helps in the persistence of the disease. PMID: 32574668 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 19, 2020 Category: Pathology Authors: Dey I, Bishayi B Tags: Exp Mol Pathol Source Type: research

Enhanced expression of lncRNA ZXF1 promotes cisplatin resistance in lung cancer cell via MAPK axis.
Abstract Long non-coding RNA (lncRNA) ZXF1 has recently been associated with the poor prognosis of lung cancer by promoting metastasis. However, little is known regarding the role of ZXF1 in lung cancer treatment and the underlying mechanism. Here, using lung cancer tissue and chemoresistant lung cancer cells, we investigated the interaction of ZXF1 with the efficacy of cisplatin, the first-line chemotherapy for lung cancer. We found that ZXF1 overexpression in lung cancer tissue increased the risk of treatment failure and tumor recurrence. We also provided evidence that ZXF1 contributed to cisplatin resistance an...
Source: Experimental and Molecular Pathology - June 9, 2020 Category: Pathology Authors: Yu T, Bai W, Su Y, Wang Y, Wang M, Ling C Tags: Exp Mol Pathol Source Type: research

Validation of a next generation sequencing assay for BRCA1, BRCA2, CHEK2 and PALB2 genetic testing.
In this study, we evaluated the performance of a next generation sequencing (NGS) assay for the complete analysis of BRCA1, BRCA2, CHEK2 and PALB2 genes using Agilent's SureMASTR BRCA Screen that enabled the detection of single nucleotide variants (SNVs), small insertions/deletions (indels) and copy number variations (CNVs) in a single-tube PCR based library preparation. The results showed 100% sensitivity and specificity on a set of 52 known samples from de-identified patients and external quality assessment program. A concordance rate of 87.5% was achieved in the comparison of variant classification with the external lab...
Source: Experimental and Molecular Pathology - June 8, 2020 Category: Pathology Authors: Sim WC, Lee CY, Richards R, Karolien B, Mottier V, Goh LL Tags: Exp Mol Pathol Source Type: research

Astragaloside-IV regulates endoplasmic reticulum stress-mediated neuronal apoptosis in a murine model of Parkinson's disease via the lincRNA-p21/CHOP pathway.
CONCLUSION: In this study, we found that AS-IV can lessen the expression of CHOP protein by restraining the expression of lincRNA-p21 in the PD model, thereby inhibiting neuronal apoptosis. PMID: 32511947 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 4, 2020 Category: Pathology Authors: Ge B, Li SL, Li FR Tags: Exp Mol Pathol Source Type: research

GAS5 enhances natural killer cell-mediated killing by promoting ubiquitination of SESN2 in prostate cancer cells.
CONCLUSIONS: Our findings demonstrated that GAS5 enhanced NK cell-mediated killing to PC cells by promoting degradation and ubiquitination of SESN2. PMID: 32511948 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - June 4, 2020 Category: Pathology Authors: Mu H, Li S, Xiang L, Chen C, Yu K Tags: Exp Mol Pathol Source Type: research

Anomalous expression of miR-103 in polycystic ovary syndrome influenced by hormonal, and metabolic variables.
Abstract BACKGROUND: Polycystic ovary syndrome (PCOS), a common endocrine disorder in reproductive-aged women, is correlated with obesity and insulin resistance (IR), androgens excess, chronic anovulation, and infertility. MicroRNAs (miRNAs) are small, single-stranded, noncoding RNA molecules that participate in inflammation, reproduction and metabolism, may contribute to PCOS. Current study aiming to manifest the correlation of body mass index (BMI) and testosterone (T) with miR-103 expression before and after fat loss. METHODS: 46 controls (N = 23 with BMI 
Source: Experimental and Molecular Pathology - June 2, 2020 Category: Pathology Authors: Mu J, Li Q Tags: Exp Mol Pathol Source Type: research

Mining the role of RECQL5 in gastric cancer and seeking potential regulatory network by bioinformatics analysis.
Abstract AIM: The role of RecQ protein-like 5 (RECQL5) in gastric carcinoma (GC) is still unclear. Here, we investigated the role of RECQL5 in human GC and its potential regulatory network via bioinformatics analysis. METHODS: Bioinformatic analysis was performed using data in the Oncomine database, Kaplan- Meier Plotter online software, MethHC database, catalogue of somatic mutations in cancer (COSMIC), cbioportal database and String database. Then, we verified the association between RECQL5 expression and GC prognosis by immunohistochemistry (IHC). The independent prognostic factors were determined by Cox m...
Source: Experimental and Molecular Pathology - May 31, 2020 Category: Pathology Authors: Lin Y, Chen H, Wang X, Xiang J, Wang H, Peng J Tags: Exp Mol Pathol Source Type: research

LncRNA KCNQ1OT1 contributes to cardiomyocyte apoptosis by targeting FUS in heart failure.
In conclusion, KCNQ1OT1 facilitates cardiomyocyte apoptosis by directly targeting FUS in ADR-induced HF. PMID: 32497620 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - May 31, 2020 Category: Pathology Authors: Lai L, Xu Y, Kang L, Yang J, Zhu G Tags: Exp Mol Pathol Source Type: research

Roles of a TMPO-AS1/microRNA-200c/TMEFF2 ceRNA network in the malignant behaviors and 5-FU resistance of ovarian cancer cells.
This study demonstrated that silencing of TMPO-AS1 might attenuate OC progression through inhibiting the invasion, metastasis and drug resistance of OC cells via the TMPO-AS1/miR-200c/TMEFF2 network and the disruption of the PI3K/Akt signaling pathway. PMID: 32497621 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - May 31, 2020 Category: Pathology Authors: Li H, Zhou Y, Cheng H, Tian J, Yang S Tags: Exp Mol Pathol Source Type: research