Focal adhesion kinase coordinates costamere-related JNK signaling with muscle fiber transformation after Achilles tenotomy and tendon reconstruction.
uuml;ck M Abstract Achilles tendon rupture necessitates rapid tendon reattachment to reinstate plantar flexion before affected muscles deteriorate through muscle fiber atrophy and transformation. The implicated process may involve alterations in sarcolemmal sites of myofibril attachment (costameres), which control myofibrillogenesis via a mechano-regulated mechanism through integrin-associated focal adhesion kinase (FAK). We assessed the contribution of FAK to alterations in fiber type composition and expression of costamere-associated structural proteins, the phosphorylation status of Y397-FAK and downstream mTOR...
Source: Experimental and Molecular Pathology - March 14, 2019 Category: Pathology Authors: Ferrié C, Kasper S, Wanivenhaus F, Flück M Tags: Exp Mol Pathol Source Type: research

Overexpression of survivin in pediatric Hodgkin lymphoma tumor cells: Characterization of protein expression and splice-variants transcription profile.
Abstract Survivin is abundantly expressed during fetal development but absent in most differentiated adult tissues; an exception being components of the immune system, such as B and T lymphocytes. Beyond acting as a master regulator of the cell cycle, survivin acts as an inhibitor of apoptosis and is overexpressed in almost all carcinoma types; however, its expression in lymphomas is lesser-explored. Survivin's role in carcinogenesis was subjected to its sub-cellular localization and splice transcripts expression, namely wild-type survivin, survivin-∆Ex3 and survivin-2B. To assess survivin's expression and sub-c...
Source: Experimental and Molecular Pathology - March 12, 2019 Category: Pathology Authors: Lorenzetti MA, Mosna MJ, De Matteo EN, Lombardi MG, Colli SL, Preciado MV Tags: Exp Mol Pathol Source Type: research

Ginsenoside Rh2 inhibits proliferation but promotes apoptosis and autophagy by down-regulating microRNA-638 in human retinoblastoma cells.
Abstract Even though recent therapeutic advances make retinoblastoma (RB) becomes a curable tumor, the outcome of long-term survival is poor due to the risk of developing secondary tumors. We aimed to explore the functional role of Ginsenoside Rh2 (GRh2) in human RB cells and the underlying mechanisms. Human RB Y79 and RBL-13 cells were incubated with or without GRh2, followed by assessments of cell viability, proliferation and apoptosis using Cell Counting Kit-8, bromodeoxyuridine, and flow cytometry assays. Meanwhile, expressions of proteins associated with cell cycle, apoptosis, autophagy and the phosphatidylin...
Source: Experimental and Molecular Pathology - March 7, 2019 Category: Pathology Authors: Li M, Zhang D, Cheng J, Liang J, Yu F Tags: Exp Mol Pathol Source Type: research

TRAF4 promotes endometrial cancer cell growth and migration by activation of PI3K/AKT/Oct4 signaling.
CONCLUSION: The present study demonstrated that TRAF4 may exert an oncogenic role in EC progress via its regulation of PI3K/AKT/Oct4 pathway. PMID: 30853613 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - March 7, 2019 Category: Pathology Authors: Xie P, Wang X, Kong M, Bai X, Jiang T Tags: Exp Mol Pathol Source Type: research

Quercetin ameliorates lipopolysaccharide-caused inflammatory damage via down-regulation of miR-221 in WI-38 cells.
CONCLUSION: This research verified the protective effects of quercetin on lung fibroblasts inflammatory damage. We revealed that quercetin ameliorated LPS-caused inflammatory damage of WI-38 lung fibroblasts might be through down-regulation of miR-221 and inactivation of NF-κB and JNK pathways. PMID: 30849307 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - March 6, 2019 Category: Pathology Authors: Wang C, Qu Z, Kong L, Xu L, Zhang M, Liu J, Yang Z Tags: Exp Mol Pathol Source Type: research

Down-regulation of microRNA-31-5p inhibits proliferation and invasion of osteosarcoma cells through Wnt/ β-catenin signaling pathway by enhancing AXIN1.
CONCLUSIONS: All in all, repression of miR-31-5p targets AXIN1 to activate the Wnt/β-catenin signaling pathway, thus suppressing proliferation, invasion and tumorigenicity of OS cells. PMID: 30844369 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - March 4, 2019 Category: Pathology Authors: Chen X, Zhong L, Li X, Liu W, Zhao Y, Li J Tags: Exp Mol Pathol Source Type: research

Primary sclerosing epithelioid fibrosarcoma of the kidney: Report of two additional cases with a clinicopathological and molecular cytogenetic study.
We present two cases of sclerosing epithelioid fibrosarcoma (SEF) that arose primarily in the kidney. The tumor in both cases was located at the upper pole of the kidney. Clincially, they were suspected as renal cell carcinomas. However, histological examination revealed densely hyalinized epithelioid tumor suggestive of SEF. The diffuse immunohistochemical staining of MUC4 by neoplastic cells and the presence of EWSR1 gene rearrangement by subsequent florescence in site hybridization (FISH) analysis confirmed the histological diagnosis. Molecular cytogenetic study is highly helpful in arriving at a final diagnosis, in par...
Source: Experimental and Molecular Pathology - February 27, 2019 Category: Pathology Authors: Wang X, Wang J Tags: Exp Mol Pathol Source Type: research

Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease.
Abstract The major epitopes recognized by autoantibodies in anti-glomerular basement membrane (GBM) disease are found in the α3-subunit non-collagenous domain of type IV collagen [α3(IV)NC1], which is present in the glomerular and alveolar basement membranes. These epitopes are structurally cryptic, owing to the hexamer formation of the non-collagenous domain of α3, α4, and α5 subunits and are expressed by the dissociation of the hexamer. Anti-GBM disease usually manifests as a single attack (SA), and we rarely see patients who repeatedly relapse. We recently treated a patient with an...
Source: Experimental and Molecular Pathology - February 25, 2019 Category: Pathology Authors: Nishibata Y, Masuda S, Nakazawa D, Tanaka S, Tomaru U, Nergui M, Xiao-Yu J, Cui Z, Zhao MH, Nakabayashi K, Ishizu A Tags: Exp Mol Pathol Source Type: research

Ketamine inhibits colorectal cancer cells malignant potential via blockage of NMDA receptor.
Abstract Ketamine, a common N-methyl-d-aspartate receptor (NMDAR) antagonist, is an option for cancer pain treatment in clinical practice. Ketamine has been shown to have the capacity to attenuate cancer cells malignancy. However, the underlying mechanism remains elusive. In the present study, we reported that ketamine inhibited the malignant potential of colorectal cancer cells and investigated the possible mechanisms involved. Ketamine suppressed the expression of VEGF, HIF-1α, p-AKT, p-ERK, and p-CaMK II, and reduced intracellular Ca2+ level in a concentration dependent manner (1, 5, 10 μg/ml). Furth...
Source: Experimental and Molecular Pathology - February 25, 2019 Category: Pathology Authors: Duan W, Hu J, Liu Y Tags: Exp Mol Pathol Source Type: research

GLP-1 receptor agonist impairs keratinocytes inflammatory signals by activating AMPK.
CONCLUSIONS: GLP-1 impaired keratinocytes inflammatory signals by activating AMPK and restrained macrophage migration. PMID: 30776356 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - February 15, 2019 Category: Pathology Authors: Jing Y, Zongming W, Xilin Z Tags: Exp Mol Pathol Source Type: research

p38 MAP kinases: plausible diagnostic and prognostic serum protein marker of non small cell lung cancer.
CONCLUSION: Our study revealed that, p38α is more efficient among all the isoform to predict the disease accurately and it can be concluded that p38 MAPK may be used as diagnostic as well as prognostic marker of NSCLC disease. PMID: 30771292 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - February 13, 2019 Category: Pathology Authors: Sahu V, Mohan A, Dey S Tags: Exp Mol Pathol Source Type: research

miR-26b modulates OA induced BMSC osteogenesis through regulating GSK3 β/β-catenin pathway.
CONCLUSION: Our findings indicate that miR-26b promoted BMSC osteogenesis by directly targeting GSK3β and activating canonical Wnt signal pathway, suggesting miR-26b might be serve as potential therapeutic candidate of osteoporosis. PMID: 30768922 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - February 12, 2019 Category: Pathology Authors: Hu H, Zhao C, Zhang P, Liu Y, Jiang Y, Wu E, Xue H, Liu C, Li Z Tags: Exp Mol Pathol Source Type: research

The clinical significance of endothelin receptor type B in hepatocellular carcinoma and its potential molecular mechanism.
Abstract OBJECTIVE: To explore the clinical significance and potential molecular mechanism of endothelin receptor type B (EDNRB) in hepatocellular carcinoma (HCC). METHODS: Immunohistochemistry was used to detect EDNRB protein expression level in 67 HCC paraffin embedded tissues and adjacent tissues. Correlations between EDNRB expression level and clinicopathologic parameters were analyzed in our study. The expression level and clinical significance of EDNRB in HCC were also evaluated from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database. The cBioPortal for Cancer Genomics was employe...
Source: Experimental and Molecular Pathology - February 12, 2019 Category: Pathology Authors: Zhang L, Luo B, Dang YW, He RQ, Chen G, Peng ZG, Feng ZB Tags: Exp Mol Pathol Source Type: research

Proteomic characterization of early lung response to breast cancer metastasis in mice.
CONCLUSION: The analysis enabled revealing the importance of calcium signaling during the early phase of metastasis development, early cytoskeleton and extracellular matrix reorganization, activation of defense mechanisms and metabolic adaptations. It seems that the tissue response is an interplay between pro- and anti-metastatic mechanisms accompanied by inflammation, oxidative stress and dysfunction of the barrier endothelial cells. PMID: 30763573 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - February 11, 2019 Category: Pathology Authors: Kurpińska A, Suraj J, Bonar E, Zakrzewska A, Stojak M, Sternak M, Jasztal A, Walczak M Tags: Exp Mol Pathol Source Type: research

High levels of RAI3 expression is linked to shortened survival in esophageal cancer patients.
Abstract Expression of the retinoic acid-induced protein 3 (RAI3) has been suggested to predict clinical outcome in a variety of malignancies. However, its role in esophageal cancers remains unclear. Immunohistochemical RAI3 staining was analyzed on tissue microarrays containing 359 esophageal adenocarcinomas (EAC) and 254 esophageal squamous cell carcinomas (ESCC). RAI3 immunostaining was typically absent or weakly detectable in the membranes in benign esophageal tissues. RAI3 staining was higher in malignant than in benign esophagus epithelium. High-levels of RAI3 staining were found in 79.2% of interpretable EA...
Source: Experimental and Molecular Pathology - January 29, 2019 Category: Pathology Authors: El Gammal AT, Melling N, Reeh M, Gebauer F, Mann O, Perez D, Bockhorn M, Bachmann K, Izbicki JR, Grupp K Tags: Exp Mol Pathol Source Type: research

Propofol suppresses proliferation and migration of papillary thyroid cancer cells by down-regulation of lncRNA ANRIL.
CONCLUSION: Our findings suggested the in vitro anti-tumor potential of propofol in PTC. One possible mechanism involved in the anti-tumor activity was preliminary revealed: propofol down-regulated the expression of ANRIL, and thus blocking Wnt/β-catenin and NF-κB pathways. PMID: 30703346 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 28, 2019 Category: Pathology Authors: Chen F, Li M, Zhu X Tags: Exp Mol Pathol Source Type: research

LncRNA KCNQ1OT1 promoted BMP2 expression to regulate osteogenic differentiation by sponging miRNA-214.
CONCLUSIONS: KCNQ1OT1 positively regulated osteogenic differentiation of BMSCs by acting as a ceRNA to regulate BMP2 expression through sponging miR-214. PMID: 30703347 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 28, 2019 Category: Pathology Authors: Wang CG, Liao Z, Xiao H, Liu H, Hu YH, Liao QD, Zhong D Tags: Exp Mol Pathol Source Type: research

Silence of lncRNA CHRF protects H9c2 cells against lipopolysaccharide-induced injury via up-regulating microRNA-221.
This study attempted to show the potential role of lncRNA CHRF in bacterial infection caused myocarditis. METHODS: H9c2 cells were transfected with small interfering RNAs (siRNAs) specific for lncRNA CHRF alone or in combination with miR-221 inhibitor, and then subjected to lipopolysaccharide (LPS). The following parameters were measured: cell viability, apoptosis, reactive oxygen species (ROS) generation, pro-inflammatory cytokines release, microRNA (miR)-221 expression and the activation of nuclear factor-κB (NF-κB) and c-Jun N-terminal kinase (JNK) pathways. RESULTS: Silence of lncRNA CHRF impeded ...
Source: Experimental and Molecular Pathology - January 26, 2019 Category: Pathology Authors: Zhang L, Wang L, Guo E, Qi Y Tags: Exp Mol Pathol Source Type: research

Triptolide inhibits the growth and migration of colon carcinoma cells by down-regulation of miR-191.
CONCLUSION: TPL is effective in killing CRC cells and suppressing the migratory capacity. TPL exerts anti-CRC properties possibly via down-regulating miR-191, and blocking the activation of NF-κB and Wnt/β-catenin pathways. PMID: 30684462 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 23, 2019 Category: Pathology Authors: Qi Y, Li J Tags: Exp Mol Pathol Source Type: research

Silencing of the Na+/H+ exchanger 1(NHE-1) prevents cardiac structural and functional remodeling induced by angiotensin II.
Abstract Chronic activation of the renin angiotensin system (RAS) favors several cardiac diseases, among which myocardial hypertrophy occupies an outstanding place. In this context, the hyperactivity of the cardiac Na+/H+ (NHE-1) exchanger plays a key role. The pathologic remodeling of the myocardium constitutes an independent risk factor for morbidity and mortality with continuously increasing healthcare cost. Therefore, the development of better therapeutic strategies emerges as highly mandatory. Our goal was to prevent angiotensin II (ANGII)-induced cardiac hypertrophy by NHE-1 gene silencing in Wistar rats. Th...
Source: Experimental and Molecular Pathology - January 18, 2019 Category: Pathology Authors: Medina AJ, Pinilla OA, Portiansky EL, Caldiz CI, Ennis IL Tags: Exp Mol Pathol Source Type: research

The anti-malarial drug artesunate causes cell cycle arrest and apoptosis of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells.
In this study, we demonstrate a dose- and time-dependent inhibitory effect of ART on the growth of triple-negative MDA-MB-468 and HER2-enriched SK-BR-3 breast cancer cells, which was the result of both anti-proliferative and cytotoxic activities. ART inhibited breast cancer cell proliferation via a reactive oxygen species (ROS)-dependent G2/M arrest and ROS-independent G1 arrest. ART-treated MDA-MB-468 and SK-BR-3 cells also experienced apoptotic cell death, which was both ROS- and iron-dependent. ART-induced oxidative stress caused the loss of mitochondrial outer membrane integrity and damage to the cellular DNA of MDA-MB...
Source: Experimental and Molecular Pathology - January 17, 2019 Category: Pathology Authors: Greenshields AL, Fernando W, Hoskin DW Tags: Exp Mol Pathol Source Type: research

Effects of GST null genotypes on individual susceptibility to leukemia: A meta-analysis.
Abstract BACKGROUND: Whether glutathione S-transferases (GST) null genotypes influence individual susceptibility to leukemia remains controversial. Thus, we performed this meta-analysis to better analyze potential influences of GST null genotypes on individual susceptibility to leukemia. METHODS: Literature retrieve was conducted in PubMed, Web of Science and Embase. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. RESULTS: Totally fifty-one studies were enrolled for analyses. Significant associations with elevated individual susceptibility to leukemia were detected for GSTM1 (p 
Source: Experimental and Molecular Pathology - January 16, 2019 Category: Pathology Authors: Wang J, Wu D, Sun A Tags: Exp Mol Pathol Source Type: research

Aluminum disrupts the prenatal development of the male and female gerbil prostate (Meriones unguiculatus).
This study demonstrated that body and prostatic changes were more pronounced in females than in males, and that Al performed as an endocrine-disrupting chemical in gerbils. PMID: 30659797 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 16, 2019 Category: Pathology Authors: Gomes LS, Costa JR, Campos MS, Marques MR, Biancardi MF, Taboga SR, Ghedini PC, Santos FCA Tags: Exp Mol Pathol Source Type: research

Ginsenoside Rg3 inhibits cell growth, migration and invasion in Caco-2 cells by downregulation of lncRNA CCAT1.
CONCLUSION: Ginsenoside Rg3 inhibitsmigration and invasion, and promotes apoptosis of Caco-2 cells by suppression expression of LncRNA CCAT1. PMID: 30633886 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 8, 2019 Category: Pathology Authors: Li J, Qi Y Tags: Exp Mol Pathol Source Type: research

Baicalin protects human retinal pigment epithelial cell lines against high glucose-induced cell injury by up-regulation of microRNA-145.
CONCLUSIONS: Results demonstrated that BAI exhibited the protective effect against HG-induced cell injury by up-regulation of miR-145. PMID: 30625293 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2019 Category: Pathology Authors: Dai C, Jiang S, Chu C, Xin M, Song X, Zhao B Tags: Exp Mol Pathol Source Type: research

ZEB1 mediates doxorubicin (Dox) resistance and mesenchymal characteristics of hepatocarcinoma cells.
Abstract The acquired chemoresistance during long term chemotherapy is one of the most important factors to limit the application of Doxorubicin (Dox) on clinical treatment of hepatocellular carcinoma (HCC) patients. Our present study found that Dox resistant HCC (HCC/Dox) cells had greater capability of in vitro migration and invasion compared to their parental cells. HCC/Dox cells exhibited mesenchymal characteristics, which was evidenced by the up regulation of fibronectin, vimentin while down regulation of E-Cadherin. Zeb1, one powerful epithelial mesenchymal transition related transcription factor (EMT-TF), w...
Source: Experimental and Molecular Pathology - January 4, 2019 Category: Pathology Authors: Long L, Xiang H, Liu J, Zhang Z, Sun L Tags: Exp Mol Pathol Source Type: research

Brazilein induces apoptosis and G1/G0 phase cell cycle arrest by up-regulation of miR-133a in human vestibular schwannoma cells.
CONCLUSION: In conclusion, our study demonstrated that brazilein could induce apoptosis and G1/G0 phase cell cycle arrest, and deactivate MAPK and JNK signaling pathways via up-regulation of miR-133a in human VS cells. These results provide theoretical evidence for the clinical application of brazilein and a new strategy for the treatment of acoustic neuroma. PMID: 30610843 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 2, 2019 Category: Pathology Authors: Mou Z, Wang Y, Li Y Tags: Exp Mol Pathol Source Type: research

HIVAN associated tubular pathology with reference to ER stress, mitochondrial changes, and autophagy.
Abstract Human immunodeficiency virus associated nephropathy (HIVAN) is a unique form of a renal parenchymal disorder. This disease and its characteristics can be accredited to incorporation of DNA and mRNA of human immunodeficiency virus type 1 into the renal parenchymal cells. A proper understanding of the intricacies of HIVAN and the underlying mechanisms associated with renal function and disorders is vital for the potential development of a reliable treatment for HIVAN. Specifically, the renal tubule segment of the kidney is characterized by its transport capabilities and its ability to reabsorb water and sal...
Source: Experimental and Molecular Pathology - December 31, 2018 Category: Pathology Authors: Katuri A, Bryant JL, Patel D, Patel V, Andhavarapu S, Asemu G, Davis H, Makar TK Tags: Exp Mol Pathol Source Type: research

Analytical performance evaluation of the HPV OncoCheck assay for detection of high-risk HPV infection in liquid-based cervical samples.
Abstract Recent studies have demonstrated low specificity (false positive) of human papillomavirus (HPV) DNA testing for the screening and diagnosis of cervical samples. Therefore, we evaluated the performance of the HPV OncoCheck assay, which is an HPV E6/E7 mRNA-based assay, for the detection of 16 high-risk (HR)-HPVs including HPV 16 and HPV 18 genotypes in cervical samples using multiplex reverse transcriptase-quantitative PCR. In the present study, the analytical performance of the assay was evaluated using 16 HPV single strand DNAs. Clinical evaluation was performed using 319 Thinprep® liquid-based cytol...
Source: Experimental and Molecular Pathology - December 31, 2018 Category: Pathology Authors: Wang HY, Kim H, Yoon H, Park KH, Lee H Tags: Exp Mol Pathol Source Type: research

Suppression of Tescalcin inhibits growth and metastasis in renal cell carcinoma via downregulating NHE1 and NF-kB signaling.
CONCLUSIONS: These findings demonstrate that atopic expression of Tescalcin facilitates the survival, migration and invasion of RCC cells via NHE1/pHi axis as well as AKT/ NF-κB signaling pathway, providing new perspectives for the future study of Tescalcin as a therapeutic target for RCC. PMID: 30594602 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 27, 2018 Category: Pathology Authors: Luo AJ, Tan J, He LY, Jiang XZ, Jiang ZQ, Zeng Q, Yao K, Xue J Tags: Exp Mol Pathol Source Type: research

Lycium barbarum polysaccharides alleviate hydrogen peroxide-induced injury by up-regulation of miR-4295 in human trabecular meshwork cells.
Abstract Glaucoma is a chronic neurodegenerative disease which produces damage to the optic nerve and causes sightlessness. Current remains lack of effective method for glaucoma. Lycium barbarum polysaccharides (LBPs) have pleiotropic effects on various diseases. However, the effect of LBPs on glaucoma remains unclear. The study aimed to clarify the protective effect of LBPs against hydrogen peroxide (H2O2)-induced oxidative damage in human trabecular meshwork (HTM) cells. HTM cells were exposed to H2O2 (0-400 μM) for 24 h to construct an oxidative damage model. Then, the different concentrations of LBPs (0...
Source: Experimental and Molecular Pathology - December 27, 2018 Category: Pathology Authors: Liu Y, Zhang Y Tags: Exp Mol Pathol Source Type: research

Prognostic significance of circulating tumor cells (CTCs) in Egyptian non-metastatic colorectal cancer patients: A comparative study for four different techniques of detection (Flowcytometry, CellSearch, Quantitative Real-time PCR and Cytomorphology).
Abstract PURPOSE: We assessed CTCs counts in NMCRC patients using four different techniques. METHODS: CTCs were detected in 63 NMCRC patients, 40 benign bowel diseases (BBD) and 40 normal controls (NC) using, flow-cytometry (FCM), CellSearch (CS), cytomorphology and quantitative real time (qPCR) for CK19, MUC1, CD44, CD133, ALDH1 expression. Results were correlated to progression free (PFS) and overall (OS). RESULTS: Positive CTCs (≥4 cells /7.5 mL blood) were detected in 50.8% (32/63) NMCRC by FCM and 7.5% (3/40) BBD (p 
Source: Experimental and Molecular Pathology - December 19, 2018 Category: Pathology Authors: Bahnassy AA, Salem SE, Mohanad M, Abulezz NZ, Abdellateif MS, Hussein M, Zekri CAN, Zekri AN, Allahloubi NMA Tags: Exp Mol Pathol Source Type: research

Ligustrazine promoted hypoxia-treated cell growth by upregulation of miR-135b in human umbilical vein endothelial cells.
CONCLUSION: Lig promoted hypoxia-treated HUVECs cell growth as evidenced by increasing cell viability and reducing cell apoptosis. This process might be modulated by upregulation of miR-135b and subsequent activation of JNK/SAPK and PI3K/AKT/mTOR pathways. PMID: 30576641 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 18, 2018 Category: Pathology Authors: Wei S, Wang H Tags: Exp Mol Pathol Source Type: research

LncRNA-MEG3 alleviates high glucose induced inflammation and apoptosis of retina epithelial cells via regulating miR-34a/SIRT1 axis.
CONCLUSION: MEG3 could alleviate HG-inducing apoptosis and inflammation via inhibiting NF-κB signaling pathway by targeting miR-34a/SIRT1 axis. This finding illustrated the function and mechanism of MEG3 in DR, and MEG3 might serve as potential therapeutic target for DR. PMID: 30529346 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 7, 2018 Category: Pathology Authors: Tong P, Peng QH, Gu LM, Xie WW, Li WJ Tags: Exp Mol Pathol Source Type: research

Singe nucleotide polymorphisms in osteosarcoma: Pathogenic effect and prognostic significance.
CONCLUSION: Considering the importance of SNPs in OS pathophysiology, these genetic changes may be used as potential pathogenic and prognostic biomarkers for OS. It is hoped that targeting these changes using new therapeutic approaches leads to the effective treatment of this debilitating tumor. However, better understanding of OS biology and further clinical trials are needed to achieve this goal. PMID: 30528563 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 6, 2018 Category: Pathology Authors: Asnafi AA, Behzad MM, Ghanavat M, Shahjahani M, Saki N Tags: Exp Mol Pathol Source Type: research

Anti-inflammatory and antioxidant effect of melatonin on recovery from muscular trauma induced in rats.
This study was designed to evaluate MLT action on muscle tissue in Wistar rats in an experimental model of muscle trauma. Twenty-eight Wistar rats were used, divided into four groups: CO (Control), CO + MLT (Control + Melatonin), T (Trauma) and T + MLT (Trauma + Melatonin). MLT (20 mg/kg) was administered (ip) daily at dusk until day 7. The trauma occurred on day 1, 2 h before the first MLT application. On day 8, muscle tissue was collected for histological analysis (HE), immunohistochemistry (TNF-α and NFκB), evaluation of oxidative stress through analysis of lipoperoxidation by TBARS and a...
Source: Experimental and Molecular Pathology - December 3, 2018 Category: Pathology Authors: Ostjen CA, Rosa CGS, Hartmann RM, Schemitt EG, Colares JR, Marroni NP Tags: Exp Mol Pathol Source Type: research

Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.
Abstract Uterine endometrial carcinoma is one of the common cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. However, the heterogeneity of CSCs/CICs is still elusive, and we therefore analyzed CSCs/CICs at the clonal level. We previously established sphere-cultured CSCs/CICs from primary human uterine endometrial carcinoma, and we isolated several clones from CSCs/CICs in this study. Interestingly, we established two types of clones based on the...
Source: Experimental and Molecular Pathology - November 29, 2018 Category: Pathology Authors: Tabuchi Y, Hirohashi Y, Hashimoto S, Mariya T, Asano T, Ikeo K, Kuroda T, Mizuuchi M, Murai A, Uno S, Kawai N, Kubo T, Nakatsugawa M, Kanaseki T, Tsukahara T, Saito T, Torigoe T Tags: Exp Mol Pathol Source Type: research

MiR-141-3p suppresses gastric cancer induced transition of normal fibroblast and BMSC to cancer-associated fibroblasts via targeting STAT4.
CONCLUSION: Our data showed that miR-141 inhibited migration and invasion of gastric cancer cells and inhibited transition from NFs to CAFs via targeting STAT4/wnt/β-catenin pathway. PMID: 30502321 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 28, 2018 Category: Pathology Authors: Xiao J, Zhong JH, Wei SH, Ye ZS, Gong FS, Wang C, Zeng Y, Chen LC Tags: Exp Mol Pathol Source Type: research

An unexpected diagnosis of histiocytic sarcoma.
PMID: 30502322 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 28, 2018 Category: Pathology Authors: Byers JT, French SW Tags: Exp Mol Pathol Source Type: research

Abnormal response of costal chondrocytes to acidosis in patients with chest wall deformity.
The objective of this paper was to compare internal calcium signaling in response to external pH changes in costal chondrocytes from patients with chest wall deformities and healthy individuals. Although the molecular mechanism through which chondrocytes are regulated by acidosis remains unknown, we observed reduced amplitudes of calcium rise in patient chondrocytes exposed to low pH that become further impaired upon repeat exposure. PMID: 30485799 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 25, 2018 Category: Pathology Authors: Asmar A, Semenov I, Kelly R, Stacey M Tags: Exp Mol Pathol Source Type: research

Long non-coding RNAs and cervical cancer.
Abstract Cervical cancer is determined as the second highest number of deaths factor in female cancers. Here is a need to find new biomarkers for detection and preliminary prognosis, metastasis. To find new treatment to enhance the survival of cervical cancer patients, pivotal actions are necessitated to be implemented. Long non-coding RNAs (lncRNAs) appear to be the crucial modulators in various processes and critically influence the oncogenesis. The commencement and general review actions of the following lncRNAs HOTAIR, H19, XIST, CCHE1, EBIC, MALAT1, ANRIL, LET, NEAT1, BLACAT1, UFC1, SNHG16 and SNHG20 are focu...
Source: Experimental and Molecular Pathology - November 21, 2018 Category: Pathology Authors: Aalijahan H, Ghorbian S Tags: Exp Mol Pathol Source Type: research

Knockdown on aPKC- ι inhibits epithelial-mesenchymal transition, migration and invasion of colorectal cancer cells through Rac1-JNK pathway.
CONCLUSION: Knockdown on aPKC-ι inhibited the EMT, migration and invasion of CRC cells through suppressing of Rac1-JNK pathway. Those findings indicate that aPKC-ι may serve as a novel therapeutic target for CRC. PMID: 30465755 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 19, 2018 Category: Pathology Authors: Du GS, Qiu Y, Wang WS, Peng K, Zhang ZC, Li XS, Xiao WD, Yang H Tags: Exp Mol Pathol Source Type: research

Molecular and cellular genetics of non-Hodgkin lymphoma: Diagnostic and prognostic implications.
Abstract Non-Hodgkin lymphoma (NHL) is a diverse collection of malignant neoplasms with lymphoid-cell origin which includes all the malignant lymphomas that are not classified as Hodgkin lymphoma. NHL is one of the most common types of cancer diagnosed in men and women in the developed world. In the United States of America, the past few decades have seen a significant rise in the incidence of NHL and it accounts for about 4% of all cancers now. The overall survival of NHL has improved drastically over the past ten years. This can be attributed to better understanding of pathogenesis, refined classification, enhan...
Source: Experimental and Molecular Pathology - November 19, 2018 Category: Pathology Authors: Pratap S, Scordino TS Tags: Exp Mol Pathol Source Type: research

TKS5-positive invadopodia-like structures in human tumor surgical specimens.
;az B Abstract Invadopodia, cancer cell protrusions with proteolytic activity, are functionally associated with active remodeling of the extracellular matrix. Here, we show that the invadopodia-related protein TKS5 is expressed in human pancreatic adenocarcinoma lines, and demonstrate that pancreatic cancer cells depend on TKS5 for invadopodia formation and function. Immunofluorescence staining on human pancreatic cancer cells reveals that TKS5 is a marker of mature and immature invadopodia in these cells. We analyze the co-staining patterns of TKS5 and the commonly used invadopodia marker Cortactin, and find only...
Source: Experimental and Molecular Pathology - November 12, 2018 Category: Pathology Authors: Chen YC, Baik M, Byers J, Chen KT, French SW, Díaz B Tags: Exp Mol Pathol Source Type: research

Genome-wide DNA methylation assessment of 'BRCA1-like' early-onset breast cancer: Data from the Australian Breast Cancer Family Registry.
ey MC Abstract Breast cancers arising in women carrying a germline mutation in BRCA1 are typically high-grade, early-onset and have distinct morphological features (BRCA1-like). However, the majority of early-onset breast cancers of this morphological type are not associated with germline BRCA1 mutations or constitutional BRCA1 promoter methylation. We aimed to assess DNA methylation across the genome for associations with the "BRCA1-like" morphology. Genome-wide methylation in blood-derived DNA was measured using the Infinium HumanMethylation450K BeadChip assay for women under the age of 40 years part...
Source: Experimental and Molecular Pathology - November 10, 2018 Category: Pathology Authors: Scott CM, Wong EM, Joo JE, Dugué PA, Jung CH, O'Callaghan N, Dowty J, Giles GG, Hopper JL, Southey MC Tags: Exp Mol Pathol Source Type: research

Smooth muscle cell differentiation from rabbit amniotic cells.
We examined the differentiation of AF-derived MSCs (AMSCs) into SMCs. AMSCs were sorted from cultured amniotic cells and differentiated into SMCs using differentiation agents, including platelet-derived growth factor BB (PDGF-BB) and tumor growth factor B (TGF-B). Characterization of differentiated SMCs was confirmed morphologically, molecularly (via quantitative polymerase chain reaction [qPCR] and immunocytochemistry [ICC]), and functionally (using a contractile assay and fluo-4 calcium signaling assay). Poly(lactide-co-glycolide) (PLGA) scaffolds were fabricated, and the attachment capacity of AMSCs was assessed via sca...
Source: Experimental and Molecular Pathology - November 8, 2018 Category: Pathology Authors: Senel U, Coskun OS, Tuysuz EC, Sahin M, Bayrak OF, Cakmak B, Tanriverdi HI, Kuskucu A Tags: Exp Mol Pathol Source Type: research

MicroRNA-sequencing data analyzing melanoma development and progression.
f A Abstract MicroRNAs (miRNAs) deregulated in melanoma are of growing importance in cancer research. We aimed to define the miRNAome of melanoma cell lines and primary melanocytes by RNA-Seq using identical cell lines as in a published miRNA expression study based on cDNA arrays. We identified 79 miRNAs, which are significantly deregulated during melanoma development. In addition, we could also determine 29 miRNAs being involved in melanoma progression. Interestingly, not all characterized miRNAs derived from cDNA array analyses of our and other groups could be found to be differentially expressed using RNA-Seq a...
Source: Experimental and Molecular Pathology - November 8, 2018 Category: Pathology Authors: Linck L, Liebig J, Völler D, Eichner N, Lehmann G, Meister G, Bosserhoff A Tags: Exp Mol Pathol Source Type: research

Molecular evaluation of BRAF gene mutation in thyroid tumors: Significant association with papillary tumors and extra thyroidal extension indicating its role as a biomarker of aggressive disease.
CONCLUSIONS: Pyrosequencing assay was found to be highly sensitive and accurate method for detecting BRAF point mutations. The frequency and distribution pattern of BRAF mutations is similar to global reports. Furthermore, association of BRAF mutation with extra thyroidal extension indicates its aggressive nature and thus can provide insights into the progression of thyroid tumors from less aggressive to poorly differentiated subtype. PMID: 30414980 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 8, 2018 Category: Pathology Authors: Ahmad F, Nathani R, Venkat J, Bharda A, Vanere V, Bhatia S, Das BR Tags: Exp Mol Pathol Source Type: research

Molecular biomarkers of Graves' ophthalmopathy.
Abstract Graves' ophthalmopathy (GO), a complication of Graves' disease (GD), is typified by orbital inflammation, ocular tissue expansion and remodeling and, ultimately, fibrosis. Orbital fibroblasts are key effectors of GO pathogenesis exhibiting exaggerated inflammatory and fibroproliferative responses to cytokines released by infiltrating immune cells. Activated orbital fibroblasts also produce inflammatory mediators that contribute to disease progression, facilitate the orbital trafficking of monocytes and macrophages, promote differentiation of matrix-producing myofibroblasts and stimulate accumulation of a ...
Source: Experimental and Molecular Pathology - November 8, 2018 Category: Pathology Authors: Longo CM, Higgins PJ Tags: Exp Mol Pathol Source Type: research

A-kinase anchor protein 12 (AKAP12) inhibits cell migration in breast cancer.
In this study, we evaluated the role of AKAP12 in the migration of breast cancer cells, an important cellular process in cancer progression. AKAP12 gene expression was analyzed in human breast cancer tissues using the Gene expression-based Outcome for Breast cancer Online (GOBO) database and TissueScan array, followed by relapse free survival (RFS) analysis with the Kaplan Meier Plotter. AKAP12 protein was then analyzed in normal MCF10A breast cell line and six different breast cancer cell lines (AU565, Hs578T, MCF7, MDA-MB-231, T47D and ZR751). After which, siRNA-mediated knockdown of AKAP12 was carried out in MCF10A, MDA...
Source: Experimental and Molecular Pathology - October 29, 2018 Category: Pathology Authors: Soh RYZ, Lim JP, Samy RP, Chua PJ, Bay BH Tags: Exp Mol Pathol Source Type: research