Autophagy and immune microenvironment in craniopharyngioma and ameloblastoma
CONCLUSION: This is the first study showing the presence of autophagic markers in craniopharyngiomas and describing the immune microenvironment of ameloblastomas.PMID:34655574 | DOI:10.1016/j.yexmp.2021.104712 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 16, 2021 Category: Pathology Authors: Georgia Karpathiou Mehdi Hamlat Maroa Dridi Fabien Forest Alexandra Papoudou-Bai Jean Marc Dumollard Michel Peoc'h Source Type: research

Autophagy and immune microenvironment in craniopharyngioma and ameloblastoma
CONCLUSION: This is the first study showing the presence of autophagic markers in craniopharyngiomas and describing the immune microenvironment of ameloblastomas.PMID:34655574 | DOI:10.1016/j.yexmp.2021.104712 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 16, 2021 Category: Pathology Authors: Georgia Karpathiou Mehdi Hamlat Maroa Dridi Fabien Forest Alexandra Papoudou-Bai Jean Marc Dumollard Michel Peoc'h Source Type: research

Setting a diagnostic benchmark for tumor BRCA testing: Detection of BRCA1 and BRCA2 large genomic rearrangements in FFPE tissue - A pilot study
Exp Mol Pathol. 2021 Oct 9:104705. doi: 10.1016/j.yexmp.2021.104705. Online ahead of print.ABSTRACTPARP inhibitors are used for treatment of tumors lacking function of the double-strand DNA break repair proteins BRCA1 or BRCA2 and are already approved for several cancer types. Thus, it is clinically crucial to determine germline as well as somatic BRCA1/2 mutations in those patients. The amplicon-based Oncomine BRCA1 and BRCA2 Assay is a test routinely used in diagnostics with FFPE specimens. The assay is validated for the detection of mutations, however, data on its performance in detecting large genomic rearrangements in...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Nadejda Valtcheva Bich Doan Nguyen-Str äuli Ulrich Wagner Sandra N Freiberger Zsuzsanna Varga Christian Britschgi Konstantin J Dedes Markus P Rechsteiner Source Type: research

The role of adipokines in leiomyomas development
Exp Mol Pathol. 2021 Oct 9:104693. doi: 10.1016/j.yexmp.2021.104693. Online ahead of print.ABSTRACTINTRODUCTION: Many women of premenopausal age suffer from uterine leiomyomas, which are benign tumors of the uterus. Despite the high prevalence of uterine leiomyomas underlying pathogenesis mechanisms are not fully elucidated. Early data showed a positive correlation between increased levels of adipose tissue and leiomyomas prevalence. Adipose tissue cells-adipocytes can play a potential role in leiomyomas formation by producing and secreting adipokines.AIM: The aim of this study is to summarize the current knowledge on the ...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Bogumi ła Strzałkowska Miriam Dawidowicz B łażej Ochman El żbieta Świętochowska Source Type: research

Setting a diagnostic benchmark for tumor BRCA testing: Detection of BRCA1 and BRCA2 large genomic rearrangements in FFPE tissue - A pilot study
Exp Mol Pathol. 2021 Oct 9:104705. doi: 10.1016/j.yexmp.2021.104705. Online ahead of print.ABSTRACTPARP inhibitors are used for treatment of tumors lacking function of the double-strand DNA break repair proteins BRCA1 or BRCA2 and are already approved for several cancer types. Thus, it is clinically crucial to determine germline as well as somatic BRCA1/2 mutations in those patients. The amplicon-based Oncomine BRCA1 and BRCA2 Assay is a test routinely used in diagnostics with FFPE specimens. The assay is validated for the detection of mutations, however, data on its performance in detecting large genomic rearrangements in...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Nadejda Valtcheva Bich Doan Nguyen-Str äuli Ulrich Wagner Sandra N Freiberger Zsuzsanna Varga Christian Britschgi Konstantin J Dedes Markus P Rechsteiner Source Type: research

The role of adipokines in leiomyomas development
Exp Mol Pathol. 2021 Oct 9:104693. doi: 10.1016/j.yexmp.2021.104693. Online ahead of print.ABSTRACTINTRODUCTION: Many women of premenopausal age suffer from uterine leiomyomas, which are benign tumors of the uterus. Despite the high prevalence of uterine leiomyomas underlying pathogenesis mechanisms are not fully elucidated. Early data showed a positive correlation between increased levels of adipose tissue and leiomyomas prevalence. Adipose tissue cells-adipocytes can play a potential role in leiomyomas formation by producing and secreting adipokines.AIM: The aim of this study is to summarize the current knowledge on the ...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Bogumi ła Strzałkowska Miriam Dawidowicz B łażej Ochman El żbieta Świętochowska Source Type: research

Setting a diagnostic benchmark for tumor BRCA testing: Detection of BRCA1 and BRCA2 large genomic rearrangements in FFPE tissue - A pilot study
Exp Mol Pathol. 2021 Oct 9:104705. doi: 10.1016/j.yexmp.2021.104705. Online ahead of print.ABSTRACTPARP inhibitors are used for treatment of tumors lacking function of the double-strand DNA break repair proteins BRCA1 or BRCA2 and are already approved for several cancer types. Thus, it is clinically crucial to determine germline as well as somatic BRCA1/2 mutations in those patients. The amplicon-based Oncomine BRCA1 and BRCA2 Assay is a test routinely used in diagnostics with FFPE specimens. The assay is validated for the detection of mutations, however, data on its performance in detecting large genomic rearrangements in...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Nadejda Valtcheva Bich Doan Nguyen-Str äuli Ulrich Wagner Sandra N Freiberger Zsuzsanna Varga Christian Britschgi Konstantin J Dedes Markus P Rechsteiner Source Type: research

The role of adipokines in leiomyomas development
Exp Mol Pathol. 2021 Oct 9:104693. doi: 10.1016/j.yexmp.2021.104693. Online ahead of print.ABSTRACTINTRODUCTION: Many women of premenopausal age suffer from uterine leiomyomas, which are benign tumors of the uterus. Despite the high prevalence of uterine leiomyomas underlying pathogenesis mechanisms are not fully elucidated. Early data showed a positive correlation between increased levels of adipose tissue and leiomyomas prevalence. Adipose tissue cells-adipocytes can play a potential role in leiomyomas formation by producing and secreting adipokines.AIM: The aim of this study is to summarize the current knowledge on the ...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Bogumi ła Strzałkowska Miriam Dawidowicz B łażej Ochman El żbieta Świętochowska Source Type: research

Setting a diagnostic benchmark for tumor BRCA testing: Detection of BRCA1 and BRCA2 large genomic rearrangements in FFPE tissue - A pilot study
Exp Mol Pathol. 2021 Oct 9:104705. doi: 10.1016/j.yexmp.2021.104705. Online ahead of print.ABSTRACTPARP inhibitors are used for treatment of tumors lacking function of the double-strand DNA break repair proteins BRCA1 or BRCA2 and are already approved for several cancer types. Thus, it is clinically crucial to determine germline as well as somatic BRCA1/2 mutations in those patients. The amplicon-based Oncomine BRCA1 and BRCA2 Assay is a test routinely used in diagnostics with FFPE specimens. The assay is validated for the detection of mutations, however, data on its performance in detecting large genomic rearrangements in...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Nadejda Valtcheva Bich Doan Nguyen-Str äuli Ulrich Wagner Sandra N Freiberger Zsuzsanna Varga Christian Britschgi Konstantin J Dedes Markus P Rechsteiner Source Type: research

The role of adipokines in leiomyomas development
Exp Mol Pathol. 2021 Oct 9:104693. doi: 10.1016/j.yexmp.2021.104693. Online ahead of print.ABSTRACTINTRODUCTION: Many women of premenopausal age suffer from uterine leiomyomas, which are benign tumors of the uterus. Despite the high prevalence of uterine leiomyomas underlying pathogenesis mechanisms are not fully elucidated. Early data showed a positive correlation between increased levels of adipose tissue and leiomyomas prevalence. Adipose tissue cells-adipocytes can play a potential role in leiomyomas formation by producing and secreting adipokines.AIM: The aim of this study is to summarize the current knowledge on the ...
Source: Experimental and Molecular Pathology - October 12, 2021 Category: Pathology Authors: Bogumi ła Strzałkowska Miriam Dawidowicz B łażej Ochman El żbieta Świętochowska Source Type: research

CircWHSC1 regulates malignancy and glycolysis by the miR-212-5p/AKT3 pathway in triple-negative breast cancer
CONCLUSIONS: CircWHSC1 accelerated malignancy and glycolysis of TNBC cells by the miR-212-5p/AKT3 axis. The research provided a potential prognostic biomarker and therapeutic target for TNBC.PMID:34624276 | DOI:10.1016/j.yexmp.2021.104704 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 8, 2021 Category: Pathology Authors: Li Ding Zhibing Xie Source Type: research

CircWHSC1 regulates malignancy and glycolysis by the miR-212-5p/AKT3 pathway in triple-negative breast cancer
CONCLUSIONS: CircWHSC1 accelerated malignancy and glycolysis of TNBC cells by the miR-212-5p/AKT3 axis. The research provided a potential prognostic biomarker and therapeutic target for TNBC.PMID:34624276 | DOI:10.1016/j.yexmp.2021.104704 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 8, 2021 Category: Pathology Authors: Li Ding Zhibing Xie Source Type: research

CircWHSC1 regulates malignancy and glycolysis by the miR-212-5p/AKT3 pathway in triple-negative breast cancer
CONCLUSIONS: CircWHSC1 accelerated malignancy and glycolysis of TNBC cells by the miR-212-5p/AKT3 axis. The research provided a potential prognostic biomarker and therapeutic target for TNBC.PMID:34624276 | DOI:10.1016/j.yexmp.2021.104704 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 8, 2021 Category: Pathology Authors: Li Ding Zhibing Xie Source Type: research

CircWHSC1 regulates malignancy and glycolysis by the miR-212-5p/AKT3 pathway in triple-negative breast cancer
CONCLUSIONS: CircWHSC1 accelerated malignancy and glycolysis of TNBC cells by the miR-212-5p/AKT3 axis. The research provided a potential prognostic biomarker and therapeutic target for TNBC.PMID:34624276 | DOI:10.1016/j.yexmp.2021.104704 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 8, 2021 Category: Pathology Authors: Li Ding Zhibing Xie Source Type: research

Therapeutic potentials of human microfluidic encapsulated conjunctival mesenchymal stem cells on the rat model of Parkinson's disease
CONCLUSION: It can be concluded that CJ-MSCs therapy can have protective effects against PD complications and nerve induction of cells due to their ability to express dopamine. On the other hand, CJ-MSCs microencapsulating leads to enhance even more protective effect of CJ-MSCs. However, confirmation of this hypothesis requires further studies and investigation of these cells' possible mechanisms of action.PMID:34619140 | DOI:10.1016/j.yexmp.2021.104703 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 7, 2021 Category: Pathology Authors: Meysam Forouzandeh Mohammadreza Bigdeli Hossein Mostafavi Samad Nadri Mehdi Eskandari Source Type: research

Retraction notice to "Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P" [Experimental and Molecular Pathology 117C (2020) 104529]
Exp Mol Pathol. 2021 Sep 30:104683. doi: 10.1016/j.yexmp.2021.104683. Online ahead of print.NO ABSTRACTPMID:34602257 | DOI:10.1016/j.yexmp.2021.104683 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Gang Li Pang Du Xu Qiang Dayong Jin Haochen Liu Bo Li Jianjun Guo Source Type: research

Pimitespib is effective on cecal GIST in a mouse model of familial GISTs with KIT-Asp820Tyr mutation through KIT signaling inhibition
In this report, we examined whether a heat shock protein 90 inhibitor, pimitespib (TAS-116), has an inhibitory effect on phosphorylation of KIT-Asp818Tyr and can decrease the cecal tumor volume in the model mice. First, we showed that pimitespib inhibited KIT phosphorylation both dose- and time-dependently in KIT-Asp818Tyr transfected murine Ba/F3 cells. Then, four 1-week courses of pimitespib were orally administered to heterozygous (KIT-Asp818Tyr/+) model mice. Each course consisted of once-daily administration for consecutive 5 days followed by 2 days-off. Cecal tumors were dissected, and tumor volume was histologically...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Takako Kihara Jiayin Yuan Tadashi Watabe Kazuhiro Kitajima Neinei Kimura Mizuka Ohkouchi Yuka Hashikura Shuichi Ohkubo Tsuyoshi Takahashi Seiichi Hirota Source Type: research

HSPB8 promoted intrahepatic cholangiocarcinoma progression by enhancing epithelial-mesenchymal transition and autophagy
This study studied the function of HSPB8 in ICC progression.METHODS: ICC patients (n = 150) were enrolled. The relationship between clinicopathological characteristics and HSPB8 expression was analyzed. RBE cells were transfected and treated by 3-MA. The RBE cells morphology was observed under a transmission electron microscope. Cell counting kit-8 assay, wound healing assay and Transwell experiment was conducted to detect RBE cells proliferation, migration and invasion. Quantitative reverse transcription-polymerase chain reaction, immunohistochemistry, Western blot and immunofluorescence were used for genes detection in c...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Bo Shu Yingxia Zhou Qingchun Liang Chao He Fazhao Li Source Type: research

Retraction notice to "Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P" [Experimental and Molecular Pathology 117C (2020) 104529]
Exp Mol Pathol. 2021 Sep 30:104683. doi: 10.1016/j.yexmp.2021.104683. Online ahead of print.NO ABSTRACTPMID:34602257 | DOI:10.1016/j.yexmp.2021.104683 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Gang Li Pang Du Xu Qiang Dayong Jin Haochen Liu Bo Li Jianjun Guo Source Type: research

Pimitespib is effective on cecal GIST in a mouse model of familial GISTs with KIT-Asp820Tyr mutation through KIT signaling inhibition
In this report, we examined whether a heat shock protein 90 inhibitor, pimitespib (TAS-116), has an inhibitory effect on phosphorylation of KIT-Asp818Tyr and can decrease the cecal tumor volume in the model mice. First, we showed that pimitespib inhibited KIT phosphorylation both dose- and time-dependently in KIT-Asp818Tyr transfected murine Ba/F3 cells. Then, four 1-week courses of pimitespib were orally administered to heterozygous (KIT-Asp818Tyr/+) model mice. Each course consisted of once-daily administration for consecutive 5 days followed by 2 days-off. Cecal tumors were dissected, and tumor volume was histologically...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Takako Kihara Jiayin Yuan Tadashi Watabe Kazuhiro Kitajima Neinei Kimura Mizuka Ohkouchi Yuka Hashikura Shuichi Ohkubo Tsuyoshi Takahashi Seiichi Hirota Source Type: research

HSPB8 promoted intrahepatic cholangiocarcinoma progression by enhancing epithelial-mesenchymal transition and autophagy
This study studied the function of HSPB8 in ICC progression.METHODS: ICC patients (n = 150) were enrolled. The relationship between clinicopathological characteristics and HSPB8 expression was analyzed. RBE cells were transfected and treated by 3-MA. The RBE cells morphology was observed under a transmission electron microscope. Cell counting kit-8 assay, wound healing assay and Transwell experiment was conducted to detect RBE cells proliferation, migration and invasion. Quantitative reverse transcription-polymerase chain reaction, immunohistochemistry, Western blot and immunofluorescence were used for genes detection in c...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Bo Shu Yingxia Zhou Qingchun Liang Chao He Fazhao Li Source Type: research

Retraction notice to "Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P" [Experimental and Molecular Pathology 117C (2020) 104529]
Exp Mol Pathol. 2021 Sep 30:104683. doi: 10.1016/j.yexmp.2021.104683. Online ahead of print.NO ABSTRACTPMID:34602257 | DOI:10.1016/j.yexmp.2021.104683 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Gang Li Pang Du Xu Qiang Dayong Jin Haochen Liu Bo Li Jianjun Guo Source Type: research

Pimitespib is effective on cecal GIST in a mouse model of familial GISTs with KIT-Asp820Tyr mutation through KIT signaling inhibition
In this report, we examined whether a heat shock protein 90 inhibitor, pimitespib (TAS-116), has an inhibitory effect on phosphorylation of KIT-Asp818Tyr and can decrease the cecal tumor volume in the model mice. First, we showed that pimitespib inhibited KIT phosphorylation both dose- and time-dependently in KIT-Asp818Tyr transfected murine Ba/F3 cells. Then, four 1-week courses of pimitespib were orally administered to heterozygous (KIT-Asp818Tyr/+) model mice. Each course consisted of once-daily administration for consecutive 5 days followed by 2 days-off. Cecal tumors were dissected, and tumor volume was histologically...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Takako Kihara Jiayin Yuan Tadashi Watabe Kazuhiro Kitajima Neinei Kimura Mizuka Ohkouchi Yuka Hashikura Shuichi Ohkubo Tsuyoshi Takahashi Seiichi Hirota Source Type: research

HSPB8 promoted intrahepatic cholangiocarcinoma progression by enhancing epithelial-mesenchymal transition and autophagy
This study studied the function of HSPB8 in ICC progression.METHODS: ICC patients (n = 150) were enrolled. The relationship between clinicopathological characteristics and HSPB8 expression was analyzed. RBE cells were transfected and treated by 3-MA. The RBE cells morphology was observed under a transmission electron microscope. Cell counting kit-8 assay, wound healing assay and Transwell experiment was conducted to detect RBE cells proliferation, migration and invasion. Quantitative reverse transcription-polymerase chain reaction, immunohistochemistry, Western blot and immunofluorescence were used for genes detection in c...
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Bo Shu Yingxia Zhou Qingchun Liang Chao He Fazhao Li Source Type: research

Retraction notice to "Low-expressed GAS5 injure myocardial cells and progression of chronic heart failure via regulation of miR-223-3P" [Experimental and Molecular Pathology 117C (2020) 104529]
Exp Mol Pathol. 2021 Sep 30:104683. doi: 10.1016/j.yexmp.2021.104683. Online ahead of print.NO ABSTRACTPMID:34602257 | DOI:10.1016/j.yexmp.2021.104683 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - October 4, 2021 Category: Pathology Authors: Gang Li Pang Du Xu Qiang Dayong Jin Haochen Liu Bo Li Jianjun Guo Source Type: research

The role of cell surface proteins gene expression in diagnosis, prognosis, and drug resistance of colorectal cancer: In silico analysis and validation
This study aimed to distinguish overexpressed CSPs in colorectal cancer to investigate their biomarker, prognosis, and drug resistance potential. Raw data of three datasets including 1187 samples was downloaded then normalization and differential expression were performed. By the combination of the cancer genome atlas (TCGA) clinical data, survival analysis was carried out. Information of all CSPs was collected from cell surface protein atlas. The role of each candidate gene expression was investigated in drug resistance by CCEL and GDSC data from PharmacoGX. CRC samples including 30 tumor samples and adjacent normal were ...
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Nasrin Nazempour Mohammad Hossein Taleqani Navid Taheri Amir Hossein Haji Ali Asgary Najafabadi Alireza Shokrollahi Atefeh Zamani Nasrin Fattahi Dolatabadi Maryam Peymani Mohammad Mahdevar Source Type: research

Nodular fasciitis of the breast: Report of two cases illustrating the diagnostic implications for USP6 gene rearrangement and brief review of the literature
In this study, we report 2 cases of nodular fasciitis occurring in the female breast, reviewing the literature and emphasizing the application of fluorescence in situ hybridization analysis of USP6 gene rearrangement in its diagnosis and differential diagnosis.PMID:34592198 | DOI:10.1016/j.yexmp.2021.104690 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Ming Zhao Xiaona Yin Yang Wen Guoqing Ru Xuli Meng Source Type: research

Higher detection of JC polyomavirus in colorectal cancerous tissue after pretreatment with topoisomerase I enzyme; colorectal tissue serves as a JCPyV persistence site
CONCLUSIONS: The presence of JCPyV in CRC tissues, as well as T-Ag localization in the nucleolus, where its oncogenic effect takes place, may provide supporting evidence for JCPyV involvement in CRC development. The study highlights the importance of using topoisomerase I to enhance JCPyV genome detection. Also, colorectal tissue is one of the permissive human tissue for JC resistance after preliminary infection.PMID:34592199 | DOI:10.1016/j.yexmp.2021.104687 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Samira Izi Masoud Youssefi Nema Mohammadian Roshan Amir Azimian Saeid Amel Jamehdar Farnaz Zahedi Avval Source Type: research

Expression of mBD4, mBD3 and CRAMP during type II collagen-induced arthritis/CIA and their association with inflammation and bone-remodeling markers
CONCLUSIONS: Our results demonstrate that CRAMP plays an important role in CIA progress and suggest that its abundance is associated with local pro- and anti-inflammatory status. This makes us propose CRAMP as a possible contributor of bone reconstruction in the last stage of CIA.PMID:34592200 | DOI:10.1016/j.yexmp.2021.104689 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: G Mendez-Frausto E E Uresti-Rivera S Godina-Gonzalez D P Portales-Perez R Gonzalez-Amaro M H Garcia-Hernandez Source Type: research

The role of cell surface proteins gene expression in diagnosis, prognosis, and drug resistance of colorectal cancer: In silico analysis and validation
This study aimed to distinguish overexpressed CSPs in colorectal cancer to investigate their biomarker, prognosis, and drug resistance potential. Raw data of three datasets including 1187 samples was downloaded then normalization and differential expression were performed. By the combination of the cancer genome atlas (TCGA) clinical data, survival analysis was carried out. Information of all CSPs was collected from cell surface protein atlas. The role of each candidate gene expression was investigated in drug resistance by CCEL and GDSC data from PharmacoGX. CRC samples including 30 tumor samples and adjacent normal were ...
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Nasrin Nazempour Mohammad Hossein Taleqani Navid Taheri Amir Hossein Haji Ali Asgary Najafabadi Alireza Shokrollahi Atefeh Zamani Nasrin Fattahi Dolatabadi Maryam Peymani Mohammad Mahdevar Source Type: research

Nodular fasciitis of the breast: Report of two cases illustrating the diagnostic implications for USP6 gene rearrangement and brief review of the literature
In this study, we report 2 cases of nodular fasciitis occurring in the female breast, reviewing the literature and emphasizing the application of fluorescence in situ hybridization analysis of USP6 gene rearrangement in its diagnosis and differential diagnosis.PMID:34592198 | DOI:10.1016/j.yexmp.2021.104690 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Ming Zhao Xiaona Yin Yang Wen Guoqing Ru Xuli Meng Source Type: research

Higher detection of JC polyomavirus in colorectal cancerous tissue after pretreatment with topoisomerase I enzyme; colorectal tissue serves as a JCPyV persistence site
CONCLUSIONS: The presence of JCPyV in CRC tissues, as well as T-Ag localization in the nucleolus, where its oncogenic effect takes place, may provide supporting evidence for JCPyV involvement in CRC development. The study highlights the importance of using topoisomerase I to enhance JCPyV genome detection. Also, colorectal tissue is one of the permissive human tissue for JC resistance after preliminary infection.PMID:34592199 | DOI:10.1016/j.yexmp.2021.104687 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Samira Izi Masoud Youssefi Nema Mohammadian Roshan Amir Azimian Saeid Amel Jamehdar Farnaz Zahedi Avval Source Type: research

Expression of mBD4, mBD3 and CRAMP during type II collagen-induced arthritis/CIA and their association with inflammation and bone-remodeling markers
CONCLUSIONS: Our results demonstrate that CRAMP plays an important role in CIA progress and suggest that its abundance is associated with local pro- and anti-inflammatory status. This makes us propose CRAMP as a possible contributor of bone reconstruction in the last stage of CIA.PMID:34592200 | DOI:10.1016/j.yexmp.2021.104689 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: G Mendez-Frausto E E Uresti-Rivera S Godina-Gonzalez D P Portales-Perez R Gonzalez-Amaro M H Garcia-Hernandez Source Type: research

The role of cell surface proteins gene expression in diagnosis, prognosis, and drug resistance of colorectal cancer: In silico analysis and validation
This study aimed to distinguish overexpressed CSPs in colorectal cancer to investigate their biomarker, prognosis, and drug resistance potential. Raw data of three datasets including 1187 samples was downloaded then normalization and differential expression were performed. By the combination of the cancer genome atlas (TCGA) clinical data, survival analysis was carried out. Information of all CSPs was collected from cell surface protein atlas. The role of each candidate gene expression was investigated in drug resistance by CCEL and GDSC data from PharmacoGX. CRC samples including 30 tumor samples and adjacent normal were ...
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Nasrin Nazempour Mohammad Hossein Taleqani Navid Taheri Amir Hossein Haji Ali Asgary Najafabadi Alireza Shokrollahi Atefeh Zamani Nasrin Fattahi Dolatabadi Maryam Peymani Mohammad Mahdevar Source Type: research

Nodular fasciitis of the breast: Report of two cases illustrating the diagnostic implications for USP6 gene rearrangement and brief review of the literature
In this study, we report 2 cases of nodular fasciitis occurring in the female breast, reviewing the literature and emphasizing the application of fluorescence in situ hybridization analysis of USP6 gene rearrangement in its diagnosis and differential diagnosis.PMID:34592198 | DOI:10.1016/j.yexmp.2021.104690 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Ming Zhao Xiaona Yin Yang Wen Guoqing Ru Xuli Meng Source Type: research

Higher detection of JC polyomavirus in colorectal cancerous tissue after pretreatment with topoisomerase I enzyme; colorectal tissue serves as a JCPyV persistence site
CONCLUSIONS: The presence of JCPyV in CRC tissues, as well as T-Ag localization in the nucleolus, where its oncogenic effect takes place, may provide supporting evidence for JCPyV involvement in CRC development. The study highlights the importance of using topoisomerase I to enhance JCPyV genome detection. Also, colorectal tissue is one of the permissive human tissue for JC resistance after preliminary infection.PMID:34592199 | DOI:10.1016/j.yexmp.2021.104687 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: Samira Izi Masoud Youssefi Nema Mohammadian Roshan Amir Azimian Saeid Amel Jamehdar Farnaz Zahedi Avval Source Type: research

Expression of mBD4, mBD3 and CRAMP during type II collagen-induced arthritis/CIA and their association with inflammation and bone-remodeling markers
CONCLUSIONS: Our results demonstrate that CRAMP plays an important role in CIA progress and suggest that its abundance is associated with local pro- and anti-inflammatory status. This makes us propose CRAMP as a possible contributor of bone reconstruction in the last stage of CIA.PMID:34592200 | DOI:10.1016/j.yexmp.2021.104689 (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - September 30, 2021 Category: Pathology Authors: G Mendez-Frausto E E Uresti-Rivera S Godina-Gonzalez D P Portales-Perez R Gonzalez-Amaro M H Garcia-Hernandez Source Type: research

Validation of a next-generation sequencing assay for the detection of EGFR mutations in cell-free circulating tumor DNA
Exp Mol Pathol. 2021 Sep 21:104685. doi: 10.1016/j.yexmp.2021.104685. Online ahead of print.ABSTRACTDetection of EGFR mutations from circulating tumor DNA (ctDNA) from blood plasma represents a gentle, non-invasive alternative to rebiopsy and can therefore be used for therapy monitoring of non-small-cell lung cancer (NSCLC) patients. The aim of this project was to investigate whether the Reveal ctDNA™ 28 NGS assay (ArcherDX, Boulder, CO), has a comparable sensitivity and specificity to droplet digital PCR (ddPCR, gold-standard) and is therefore suitable for therapy monitoring of progressing lung cancer patients. Firs...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Regina Stitz Anna Buder Rene Silye Bernhard Baumgartner Franz P ühringer Martin Filipits Eva Oberndorfer Ellen Heitzer Source Type: research

A PHF1-TFE3 fusion atypical ossifying fibromyxoid tumor with prominent collagenous rosettes: Case report with a brief review
Exp Mol Pathol. 2021 Sep 21:104686. doi: 10.1016/j.yexmp.2021.104686. Online ahead of print.ABSTRACTOssifying fibromyxoid tumor (OFMT) is a rare mesenchymal neoplasm of uncertain line of differentiation that can be subdivided into typical, atypical, and malignant tumors. Cytogenetically, OFMT is characterized by recurrent gene rearrangement involving PHF1 in up to 85% of cases. The most common PHF1 fusion partner is EP400, present in approximately half of cases. Most recently, a novel fusion of PHF1-TFE3 was identified in about 10% of PHF1-rearranged OFMTs. Herein, we report a unique case of PHF1-TFE3 fusion atypical OFMT ...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Cheng Zou Guo-Qing Ru Ming Zhao Source Type: research

Validation of a next-generation sequencing assay for the detection of EGFR mutations in cell-free circulating tumor DNA
Exp Mol Pathol. 2021 Sep 21:104685. doi: 10.1016/j.yexmp.2021.104685. Online ahead of print.ABSTRACTDetection of EGFR mutations from circulating tumor DNA (ctDNA) from blood plasma represents a gentle, non-invasive alternative to rebiopsy and can therefore be used for therapy monitoring of non-small-cell lung cancer (NSCLC) patients. The aim of this project was to investigate whether the Reveal ctDNA™ 28 NGS assay (ArcherDX, Boulder, CO), has a comparable sensitivity and specificity to droplet digital PCR (ddPCR, gold-standard) and is therefore suitable for therapy monitoring of progressing lung cancer patients. Firs...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Regina Stitz Anna Buder Rene Silye Bernhard Baumgartner Franz P ühringer Martin Filipits Eva Oberndorfer Ellen Heitzer Source Type: research

A PHF1-TFE3 fusion atypical ossifying fibromyxoid tumor with prominent collagenous rosettes: Case report with a brief review
Exp Mol Pathol. 2021 Sep 21:104686. doi: 10.1016/j.yexmp.2021.104686. Online ahead of print.ABSTRACTOssifying fibromyxoid tumor (OFMT) is a rare mesenchymal neoplasm of uncertain line of differentiation that can be subdivided into typical, atypical, and malignant tumors. Cytogenetically, OFMT is characterized by recurrent gene rearrangement involving PHF1 in up to 85% of cases. The most common PHF1 fusion partner is EP400, present in approximately half of cases. Most recently, a novel fusion of PHF1-TFE3 was identified in about 10% of PHF1-rearranged OFMTs. Herein, we report a unique case of PHF1-TFE3 fusion atypical OFMT ...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Cheng Zou Guo-Qing Ru Ming Zhao Source Type: research

Validation of a next-generation sequencing assay for the detection of EGFR mutations in cell-free circulating tumor DNA
Exp Mol Pathol. 2021 Sep 21:104685. doi: 10.1016/j.yexmp.2021.104685. Online ahead of print.ABSTRACTDetection of EGFR mutations from circulating tumor DNA (ctDNA) from blood plasma represents a gentle, non-invasive alternative to rebiopsy and can therefore be used for therapy monitoring of non-small-cell lung cancer (NSCLC) patients. The aim of this project was to investigate whether the Reveal ctDNA™ 28 NGS assay (ArcherDX, Boulder, CO), has a comparable sensitivity and specificity to droplet digital PCR (ddPCR, gold-standard) and is therefore suitable for therapy monitoring of progressing lung cancer patients. Firs...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Regina Stitz Anna Buder Rene Silye Bernhard Baumgartner Franz P ühringer Martin Filipits Eva Oberndorfer Ellen Heitzer Source Type: research

A PHF1-TFE3 fusion atypical ossifying fibromyxoid tumor with prominent collagenous rosettes: Case report with a brief review
Exp Mol Pathol. 2021 Sep 21:104686. doi: 10.1016/j.yexmp.2021.104686. Online ahead of print.ABSTRACTOssifying fibromyxoid tumor (OFMT) is a rare mesenchymal neoplasm of uncertain line of differentiation that can be subdivided into typical, atypical, and malignant tumors. Cytogenetically, OFMT is characterized by recurrent gene rearrangement involving PHF1 in up to 85% of cases. The most common PHF1 fusion partner is EP400, present in approximately half of cases. Most recently, a novel fusion of PHF1-TFE3 was identified in about 10% of PHF1-rearranged OFMTs. Herein, we report a unique case of PHF1-TFE3 fusion atypical OFMT ...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Cheng Zou Guo-Qing Ru Ming Zhao Source Type: research

Validation of a next-generation sequencing assay for the detection of EGFR mutations in cell-free circulating tumor DNA
Exp Mol Pathol. 2021 Sep 21:104685. doi: 10.1016/j.yexmp.2021.104685. Online ahead of print.ABSTRACTDetection of EGFR mutations from circulating tumor DNA (ctDNA) from blood plasma represents a gentle, non-invasive alternative to rebiopsy and can therefore be used for therapy monitoring of non-small-cell lung cancer (NSCLC) patients. The aim of this project was to investigate whether the Reveal ctDNA™ 28 NGS assay (ArcherDX, Boulder, CO), has a comparable sensitivity and specificity to droplet digital PCR (ddPCR, gold-standard) and is therefore suitable for therapy monitoring of progressing lung cancer patients. Firs...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Regina Stitz Anna Buder Rene Silye Bernhard Baumgartner Franz P ühringer Martin Filipits Eva Oberndorfer Ellen Heitzer Source Type: research

A PHF1-TFE3 fusion atypical ossifying fibromyxoid tumor with prominent collagenous rosettes: Case report with a brief review
Exp Mol Pathol. 2021 Sep 21:104686. doi: 10.1016/j.yexmp.2021.104686. Online ahead of print.ABSTRACTOssifying fibromyxoid tumor (OFMT) is a rare mesenchymal neoplasm of uncertain line of differentiation that can be subdivided into typical, atypical, and malignant tumors. Cytogenetically, OFMT is characterized by recurrent gene rearrangement involving PHF1 in up to 85% of cases. The most common PHF1 fusion partner is EP400, present in approximately half of cases. Most recently, a novel fusion of PHF1-TFE3 was identified in about 10% of PHF1-rearranged OFMTs. Herein, we report a unique case of PHF1-TFE3 fusion atypical OFMT ...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Cheng Zou Guo-Qing Ru Ming Zhao Source Type: research

Validation of a next-generation sequencing assay for the detection of EGFR mutations in cell-free circulating tumor DNA
Exp Mol Pathol. 2021 Sep 21:104685. doi: 10.1016/j.yexmp.2021.104685. Online ahead of print.ABSTRACTDetection of EGFR mutations from circulating tumor DNA (ctDNA) from blood plasma represents a gentle, non-invasive alternative to rebiopsy and can therefore be used for therapy monitoring of non-small-cell lung cancer (NSCLC) patients. The aim of this project was to investigate whether the Reveal ctDNA™ 28 NGS assay (ArcherDX, Boulder, CO), has a comparable sensitivity and specificity to droplet digital PCR (ddPCR, gold-standard) and is therefore suitable for therapy monitoring of progressing lung cancer patients. Firs...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Regina Stitz Anna Buder Rene Silye Bernhard Baumgartner Franz P ühringer Martin Filipits Eva Oberndorfer Ellen Heitzer Source Type: research

A PHF1-TFE3 fusion atypical ossifying fibromyxoid tumor with prominent collagenous rosettes: Case report with a brief review
Exp Mol Pathol. 2021 Sep 21:104686. doi: 10.1016/j.yexmp.2021.104686. Online ahead of print.ABSTRACTOssifying fibromyxoid tumor (OFMT) is a rare mesenchymal neoplasm of uncertain line of differentiation that can be subdivided into typical, atypical, and malignant tumors. Cytogenetically, OFMT is characterized by recurrent gene rearrangement involving PHF1 in up to 85% of cases. The most common PHF1 fusion partner is EP400, present in approximately half of cases. Most recently, a novel fusion of PHF1-TFE3 was identified in about 10% of PHF1-rearranged OFMTs. Herein, we report a unique case of PHF1-TFE3 fusion atypical OFMT ...
Source: Experimental and Molecular Pathology - September 24, 2021 Category: Pathology Authors: Cheng Zou Guo-Qing Ru Ming Zhao Source Type: research

Expression of immunoproteasome subunits in the brains of Toxoplasma gondii-infected mice
In this study, we established an in vivo mouse model of T. gondii PLK strain infection via intraperitoneal injection and evaluated the expression of immunoproteasome subunits in the brains of infected mice. The results demonstrated that first, pathological changes in the brains of infected mice increase in severity over time. Second, following T. gondii infection, activated microglia and astrocytes undergo a series of functional alterations and morphological transformations, including proliferation and migration. Third, T. gondii infection induces expression of inflammatory cytokines, including IFN-γ, IL-1β, TNF...
Source: Experimental and Molecular Pathology - September 21, 2021 Category: Pathology Authors: Yongli Zhang Wei Hu Qiang Liu Zelin Ma Shouping Hu Zhuo Zhang HongLin Jia Xijun He Source Type: research

Expression of immunoproteasome subunits in the brains of Toxoplasma gondii-infected mice
In this study, we established an in vivo mouse model of T. gondii PLK strain infection via intraperitoneal injection and evaluated the expression of immunoproteasome subunits in the brains of infected mice. The results demonstrated that first, pathological changes in the brains of infected mice increase in severity over time. Second, following T. gondii infection, activated microglia and astrocytes undergo a series of functional alterations and morphological transformations, including proliferation and migration. Third, T. gondii infection induces expression of inflammatory cytokines, including IFN-γ, IL-1β, TNF...
Source: Experimental and Molecular Pathology - September 21, 2021 Category: Pathology Authors: Yongli Zhang Wei Hu Qiang Liu Zelin Ma Shouping Hu Zhuo Zhang HongLin Jia Xijun He Source Type: research

Expression of immunoproteasome subunits in the brains of Toxoplasma gondii-infected mice
In this study, we established an in vivo mouse model of T. gondii PLK strain infection via intraperitoneal injection and evaluated the expression of immunoproteasome subunits in the brains of infected mice. The results demonstrated that first, pathological changes in the brains of infected mice increase in severity over time. Second, following T. gondii infection, activated microglia and astrocytes undergo a series of functional alterations and morphological transformations, including proliferation and migration. Third, T. gondii infection induces expression of inflammatory cytokines, including IFN-γ, IL-1β, TNF...
Source: Experimental and Molecular Pathology - September 21, 2021 Category: Pathology Authors: Yongli Zhang Wei Hu Qiang Liu Zelin Ma Shouping Hu Zhuo Zhang HongLin Jia Xijun He Source Type: research