The clinicomolecular landscape of de novo versus relapsed stage IV metastatic breast cancer.
Abstract BACKGROUND: de novo metastatic breast cancer (dnMBC) is responsible for 6-10% of breast cancer presentations with increasing incidence and has remained resistant to detection by mammography screening. Recent publications hypothesized that in addition to poor screening uptake, the presentation of dnMBC may be due to its unfavourable biology which remains unknown at the molecular level. Here we investigated the tumour biology of dnMBC in the form of clinicopathology, genomic alterations and differential gene expression to create a comparative landscape of de novo versus relapsed metastatic breast cancer (rM...
Source: Experimental and Molecular Pathology - February 14, 2020 Category: Pathology Authors: Seltzer S, Corrigan M, O'Reilly S Tags: Exp Mol Pathol Source Type: research

CD4+ T cells and TGF β1/MAPK signal pathway involved in the valvular hyperblastosis and fibrosis in patients with rheumatic heart disease.
CD4+ T cells and TGFβ1/MAPK signal pathway involved in the valvular hyperblastosis and fibrosis in patients with rheumatic heart disease. Exp Mol Pathol. 2020 Feb 13;:104402 Authors: Zhao Z, He D, Ling F, Chu T, Huang D, Wu H, Ge J Abstract The aim of this study was to investigate the roles of CD4+ T cells and transforming growth factor beta (TGFβ1) in the pathological process of valvular hyperblastosis and fibrosis of patients with rheumatic heart disease (RHD). A total of 151 patients were enrolled, among whom, 78 patients were with RHD, and 73 were age and gender matched RHD negative pati...
Source: Experimental and Molecular Pathology - February 13, 2020 Category: Pathology Authors: Zhao Z, He D, Ling F, Chu T, Huang D, Wu H, Ge J Tags: Exp Mol Pathol Source Type: research

Effects of acute noise exposure on DNA damage response genes in the cochlea, cortex, heart and liver.
Abstract Noise as a systemic stressor induces various organ dysfunctions and the underlying molecular pathology is unknown. Previous studies have shown that noise exposure results in the accumulation of DNA damage in auditory and non-auditory organs. The DNA damage response (DDR) is a global protective mechanism that plays a critical role in maintaining DNA integrity. However, the role of DDR genes in noise induced systemic (non-auditory) pathology has not been investigated. The current pilot study was designed to test the hypothesis that an acute noise exposure would alter the normal expression of DDR genes (e.g....
Source: Experimental and Molecular Pathology - February 13, 2020 Category: Pathology Authors: Yang L, Guthrie OW Tags: Exp Mol Pathol Source Type: research

Feasibility and clinical utility of a pan-solid tumor targeted RNA fusion panel: A single center experience.
We present our experience with the validation of a custom-designed Archer Anchored Multiplex PCR (AMP™) technology-based NGS technology, "NYU FUSION-SEQer" using RNA sequencing. We examine both analytical performance and clinical utility of the panel using 75 retrospective validation samples and 84 prospective clinical samples of solid tumors. Our panel showed robust sequencing performance with strong enrichment for target regions. The lower limit of detection was 12.5% tumor fraction at 125 ng of RNA input. The panel demonstrated excellent analytic accuracy, with 100% sensitivity, 100% specificity and 10...
Source: Experimental and Molecular Pathology - February 13, 2020 Category: Pathology Authors: Hindi I, Shen G, Tan Q, Cotzia P, Snuderl M, Feng X, Jour G Tags: Exp Mol Pathol Source Type: research

Comparative expression of immunohistochemical biomarkers in cribriform and pattern 4 non-cribriform prostatic adenocarcinoma.
Abstract The morphology of Gleason 4 prostate cancer (PCa) can be subdivided into cribriform and non-cribriform patterns. A large body of evidence has shown that pattern 4 cribriform PCa (especially non-glomeruloid type) is associated with adverse pathologic features and clinical outcomes compared with non-cribriform pattern 4 PCa. The underlying mechanisms for the aggressiveness of cribriform PCa are not fully understood. The aim of this study is to compare the immunohistochemical expression of various biomarkers and to determine the potential proteins that may account for their biologic and clinical differences....
Source: Experimental and Molecular Pathology - February 12, 2020 Category: Pathology Authors: Xiao GQ, Nguyen E, Unger PD, Sherrod AE Tags: Exp Mol Pathol Source Type: research

Dual biomarkers long non-coding RNA GAS5 and its target, NR3C1, contribute to acute myeloid leukemia.
Abstract Acute myeloid leukemia (AML) is a complex hematological neoplasm with poor prognosis. At present, overwhelming evidence indicates that different genetic abnormalities are relevant to the pathogenesis of AML. Nevertheless, its exact molecular mechanism is still unknown. Recently, it was reported that lncRNAs play crucial roles in tumorigenesis. But, their role in the molecular pathogenesis of AML has not been extensively explored. GAS5, one of the earliest known lncRNAs, has an essential role in the formation and progression of multiple human cancers. It was recently demonstrated that GAS5 acts as a ribore...
Source: Experimental and Molecular Pathology - February 4, 2020 Category: Pathology Authors: Ketab FNG, Gharesouran J, Ghafouri-Fard S, Dastar S, Mazraeh SA, Hosseinzadeh H, Moradi M, Javadlar M, Hiradfar A, Rezamand A, Taheri M, Rezazadeh M Tags: Exp Mol Pathol Source Type: research

SFRP1 promotor methylation analysis of FTA card touch-prep samples derived from colonic polyps.
;user G Abstract Whatman FTA® cards provide the most reliable method for DNA storage and extraction, however, the literature lacks reports on the epigenetic analysis of FTA card-derived tumor DNA. Therefore, this study aimed at demonstrating that punches from colonic adenoma samples preserved on FTA filter cards are suitable for methylation analysis by real-time methylation-specific PCR (MSP). Genomic DNA was isolated from a total of 40 sporadic colorectal adenoma samples stored on FTA cards for a median of 59.60 (range 48-72) months. After bisulfite treatment, deaminated DNA was analyzed by SYBR Green real-ti...
Source: Experimental and Molecular Pathology - January 30, 2020 Category: Pathology Authors: Buxhofer-Ausch V, Ausch C, Reiner A, Müllner-Ammer K, Schmid A, Kriwanek S, Sebesta C, Halwachs-Baumann G, Kriegshäuser G Tags: Exp Mol Pathol Source Type: research

Siglec1 enhances inflammation through miR-1260-dependent degradation of I κBα in COPD.
Siglec1 enhances inflammation through miR-1260-dependent degradation of IκBα in COPD. Exp Mol Pathol. 2020 Jan 30;:104398 Authors: Li S, Jiang L, Yang Y, Cao J, Zhang Q, Zhang J, Wang R, Deng X, Li Y Abstract It has been documented that sialic acid-binding Ig-like lectin 1 (Siglec1) is a cell surface protein with a variety of functions in the immune system. In the present study, we evaluated whether Siglec1 plays a role in chronic obstructive pulmonary disease (COPD). Results show that the expression of Siglec1 was increased in the lung of COPD rats, and that Siglec1 overexpression greatly...
Source: Experimental and Molecular Pathology - January 30, 2020 Category: Pathology Authors: Li S, Jiang L, Yang Y, Cao J, Zhang Q, Zhang J, Wang R, Deng X, Li Y Tags: Exp Mol Pathol Source Type: research

Analyses of VEGFC/VEGF-D expressions, density and endothelial lymphatic proliferation in salivary gland neoplasms.
CONCLUSION: No correlation was detected between VEGF-C and VEGF-D immunoexpression in relation to lymphatic tumor density and endothelial lymphatic proliferation. Western blotting (WB) revealed no difference between VEGF-C and VEGF-D expression among the lesions, corroborating the immunohistochemistry findings. PMID: 32004508 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 28, 2020 Category: Pathology Authors: Barroso KMA, de Andrade Santos PP, da Silva LP, Nonaka CFW, Sarmento ASC, Pinto LP, de Souza LB Tags: Exp Mol Pathol Source Type: research

miR-133 inhibits proliferation and promotes apoptosis by targeting LASP1 in lupus nephritis.
This study revealed a new mechanism involving the interaction of miR-133 and LASP1 in the pathogenesis of LN. PMID: 31987844 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 24, 2020 Category: Pathology Authors: Huang Z, Pang G, Huang YG, Li C Tags: Exp Mol Pathol Source Type: research

Expression assessment of a panel of long non-coding RNAs in gastric malignancy.
CONCLUSION: The current investigation underscores the dysregulation of these transcripts in gastric cancer specimens and suggests a number of these transcripts for further assessments of their suitability as cancer biomarkers. PMID: 31982396 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 23, 2020 Category: Pathology Authors: Esfandi F, Salehnezhad T, Taheri M, Afsharpad M, Hafez AA, Oskooei VK, Ghafouri-Fard S Tags: Exp Mol Pathol Source Type: research

The need for rapid therapeutic efficacy testing for cancer therapy.
PMID: 31982397 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 23, 2020 Category: Pathology Authors: Kaufman DG Tags: Exp Mol Pathol Source Type: research

miRNA profile in ovarian cancer.
Abstract Ovarian cancer is a gynecological cancer with high mortality and a heterogeneous nature which complicates its early detection and primary prevention. Numerous studies have evaluated expression profile microRNAs (miRNAs) in tissue and serum samples of ovarian cancer patients to find appropriate biomarkers for this malignancy. Functional experiments also verified the oncogenic or suppressor effects of a number of miRNAs. miRNAs exert their role through degradation or inhibition of translation of the target mRNA. Through this regulatory function, they modulate numerous cellular processes which are ultimately...
Source: Experimental and Molecular Pathology - January 16, 2020 Category: Pathology Authors: Ghafouri-Fard S, Shoorei H, Taheri M Tags: Exp Mol Pathol Source Type: research

Down-regulation of the human major histocompatibility complex class I chain-related gene A (MICA) and its receptor is mediated by microRNA-146b-5p and is a potential mechanism of immunoediting in papillary thyroid carcinoma.
Abstract Immune escape is one of the main reasons for the rapid progression of cancer and the poor efficacy of immunotherapy. Papillary thyroid cancer (PTC) is usually accompanied by intra-tumoral lymphocytic infiltration. The mechanisms regulating this tumor associated immune response or its evasion are not well understood. The major histocompatibility complex class I chain-related proteins A (MICA) and its receptor the natural killer group 2 member D (NKG2D) are major executers of the anti-tumor defense. This work aimed to study the expression and regulation of MICA-NKG2D and its association with the lymphocytic...
Source: Experimental and Molecular Pathology - January 11, 2020 Category: Pathology Authors: Al-Abdallah A, Jahanbani I, Mehdawi H, Ali RH, Al-Brahim N, Mojiminiyi O, Junaid TA Tags: Exp Mol Pathol Source Type: research

Knockdown of long non-coding RNA CCAT1 suppresses proliferation and EMT of human cervical cancer cell lines by down-regulating Runx2.
CONCLUSION: Knockdown of CCAT1 suppressed proliferation, EMT, migration and invasion in HeLa and SiHa cells through down-regulating Runx2, which provided theoretical support for its application in CC treatment. PMID: 31935379 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 11, 2020 Category: Pathology Authors: Li R, Liu J, Qi J Tags: Exp Mol Pathol Source Type: research

Transcriptome profiling and pathway analysis in squamous cell carcinoma of buccal mucosa.
Abstract BACKGROUND: High recurrence and poor overall survival in buccal mucosa squamous cell carcinoma (BMSCC) are not well addressed due to lack of efficient prognostic biomarkers and targeted therapies. To uncover gene candidates for the same, transcriptome profiling has been examined in BMSCC, which is not explored yet. METHODS: We compared 9 BMSCC and 2 normal oral FFPE tissues using Agilent SurePrint G3 Human gene expression v3 microarray chips. The obtained RNA signatures were interrogated in the cancer genome atlas (TCGA) dataset for alteration values and survival data. RESULTS: We found total 23...
Source: Experimental and Molecular Pathology - January 10, 2020 Category: Pathology Authors: Patel KD, Vora HH, Trivedi TI, Patel JB, Pandya SJ, Jetly DH, Patel PS Tags: Exp Mol Pathol Source Type: research

Expression of nucleostemin in odontogenic cysts and tumors.
This study showed an increased expression of nucleostemin in ameloblastoma and OKC, which suggests that stemness may be related to development of these lesions, their invasive behavior and high rate of recurrence. PMID: 31926976 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 9, 2020 Category: Pathology Authors: Andisheheh-Tadbir A, Ranjbar MA, Shiri AA, Mardani M Tags: Exp Mol Pathol Source Type: research

Assessment of CMKLR1 level in colorectal cancer and its correlation with angiogenic markers.
CONCLUSION: CMKLR1 activity may be associated with the angiogenic process in CRC via MMP-9 activity. Further research, involving a larger sample, may verify whether chemerin/CMKLR1 axis could be considered as a suitable target in novel molecular therapies. PMID: 31926977 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 9, 2020 Category: Pathology Authors: Kiczmer P, Seńkowska AP, Kula A, Dawidowicz M, Strzelczyk JK, Zajdel EN, Walkiewicz K, Waniczek D, Ostrowska Z, Świętochowska E Tags: Exp Mol Pathol Source Type: research

Effect of astaxanthin on metabolic cataract in rats with type 1 diabetes mellitus.
Abstract OBJECTIVE: The purpose of this study was to investigate the effect of astaxanthin on metabolic cataract in rats with type 1 diabetes and its antioxidant capacity to lens. METHODS: Rats were randomly divided into four groups (n = 8): control group, diabetes mellitus (DM) group, low-dose astaxanthin (DM + AL) and low-dose astaxanthin (DM + AH) group. A rat model of type I diabetes mellitus was established by intraperitoneal injection of 60 mg/kg streptozotocin (STZ). After successful modeling, rats in the administration group were given different doses of astaxanthin (AST) for 12 weeks....
Source: Experimental and Molecular Pathology - January 7, 2020 Category: Pathology Authors: Yang M, Chen Y, Zhao T, Wang Z Tags: Exp Mol Pathol Source Type: research

Icariside II attenuates eosinophils-induced airway inflammation and remodeling via inactivation of NF- κB and STAT3 in an asthma mouse model.
Icariside II attenuates eosinophils-induced airway inflammation and remodeling via inactivation of NF-κB and STAT3 in an asthma mouse model. Exp Mol Pathol. 2020 Jan 06;:104373 Authors: Tian C, Gao F, Li X, Li Z Abstract Asthma is a chronic inflammatory airway disease. Icariside II has been reported to exert anti-inflammatory effect in multiple human diseases. The present study aimed to investigate the effects and mechanisms of Icariside II on airway inflammation and remodeling in asthma. We established an asthma mouse model with ovalbumin (OVA) immunization. Histological analysis using H&E,...
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Tian C, Gao F, Li X, Li Z Tags: Exp Mol Pathol Source Type: research

Ataxia telangiectasia mutated pathway disruption affects hepatic DNA and tissue damage in nonalcoholic fatty liver disease.
Abstract To overcome the rising burdens of nonalcoholic fatty liver disease, mechanistic linkages in mitochondrial dysfunction, inflammation and hepatic injury are critical. As ataxia telangiectasia mutated (ATM) gene oversees DNA integrity and mitochondrial homeostasis, we analyzed mRNAs and total proteins or phosphoproteins by arrays in subjects with healthy liver, fatty liver or nonalcoholic steatohepatitis. Functional genomics approaches were used for DNA damage or cell growth events. The effects of fatty acid-induced toxicity in mitochondrial health, DNA integrity and cell proliferation were validated in HuH-...
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Viswanathan P, Sharma Y, Maisuradze L, Tchaikovskaya T, Gupta S Tags: Exp Mol Pathol Source Type: research

TIPE2 suppressed cisplatin resistance by inducing autophagy via mTOR signalling pathway.
In conclusion, aberrant TIPE2 expression may contribute to the occurrence of chemoresistance by interfering with autophagy in NSCLC in an mTOR-dependent manner. TIPE2 could be used as a novel therapeutic target to overcome cisplatin-resistant NSCLC. PMID: 31917287 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Guo H, Ren H, Li J, Hao M, Hao J, Ren H, Guo L, Liu R Tags: Exp Mol Pathol Source Type: research

Smad2 and Smad3 play antagonistic roles in high glucose-induced renal tubular fibrosis via the regulation of SnoN.
In this study, the expression of Smad2 and Smad3 proteins, SnoN mRNA, SnoN proteins, and the ubiquitination levels of SnoN were determined in DN rats and renal tubular epithelial cells(NRK52E cells). Knockdown and overexpression of Smad2 or Smad3 in NRK52E cells were used to investigate the specific roles of Smad2 and Smad3 in the development of high glucose-induced renal tubular fibrosis, with a specific focus on their effect on the regulation of SnoN expression. Our study demonstrated that Smad3 could inhibit SnoN expression and increase ECM deposition in NRK52E cells, to promote high glucose-induced renal tubular fibros...
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Wang Y, Zhang X, Mao Y, Liang L, Liu L, Peng W, Liu H, Xiao Y, Zhang Y, Zhang F, Shi M, Liu L, Guo B Tags: Exp Mol Pathol Source Type: research

MiR-1258 promotes the apoptosis of cervical cancer cells by regulating the E2F1/P53 signaling pathway.
CONCLUSION: Taken together, miR-1258 overexpression exerts its inhibition effects on the proliferation, invasion and migration and promotion effects on the apoptosis of cervical cancer cells by targeting the E2F1, which might provide new ideas for clinical treatment of cervical cancer. PMID: 31917289 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Peng X, Zhang Y, Gao J, Cai C Tags: Exp Mol Pathol Source Type: research

Dexmedetomidine reduces the inflammation and apoptosis of doxorubicin-induced myocardial cells.
In conclusion, this study indicated that DEX could reduce the inflammation and apoptosis of DOX-induced myocardial cells through activating the AMPK-GSK3β signaling pathway. Because of the above effects of DEX, it may be beneficial for surgical patients with heart problems. PMID: 31917290 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: He Y, Yang Z, Li J, Li E Tags: Exp Mol Pathol Source Type: research

Luteolin retards CXCL12-induced Jurkat cells migration by disrupting transcription of CXCR4.
In conclusion, luteolin exhibited anti-proliferation and anti-metastasis functions in CXCL12-treated Jurkat cells and inactivated PI3K/AKT and ERK signaling pathways via repressing the transcription of CXCR4. PMID: 31917965 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Bu Y, Zhao D Tags: Exp Mol Pathol Source Type: research

Perinatal exposure to bisphenol A impacts in the mammary gland morphology of adult Mongolian gerbils.
In conclusion, BPA perinatal exposure promotes significant changes in glandular cytoarchitecture and increases glandular epithelium proliferation rate, leading to the retention of stem-like properties. This event could compromise the fate and differentiation potential of mammary epithelium. PMID: 31917966 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - January 6, 2020 Category: Pathology Authors: Leonel ECR, Campos SGP, Bedolo CMB, Guerra LHA, Vilamaior PSL, Calmon MF, Rahal P, Amorim CA, Taboga SR Tags: Exp Mol Pathol Source Type: research

Long non-coding RNA signature in gastric cancer.
Abstract Gastric cancer as a common human malignancy has been associated with aberrant expressions of several coding and non-coding genes. Long non-coding RNAs (lncRNAs) as regulators of gene expressions at different genomic, transcriptomic and post-transcriptomic levels are among putative biomarkers and therapeutic targets in gastric cancer. In the present study, we have searched available literature and listed lncRNAs that are involved in the pathogenesis of gastric cancer. In addition, we discuss associations between expressions of these lncRNAs and tumoral features or risk factors for gastric cancer. Based on ...
Source: Experimental and Molecular Pathology - December 31, 2019 Category: Pathology Authors: Ghafouri-Fard S, Taheri M Tags: Exp Mol Pathol Source Type: research

Interference of miR-212 and miR-384 promotes osteogenic differentiation via targeting RUNX2 in osteoporosis.
Abstract The increasing level of osteogenic (OS) differentiation of bone marrow derived mesenchymal stem cells (MSCs) could be potentially used to relieve the signs and symptoms associated with osteoporosis (OP). Inhibition of osteoprotegerin (OPG)/Receptor Activator of Nuclear factor-Kappa B Ligand (RANKL) pathway plays an important role in OS differentiation, leading to excessive osteoclasts and reduction of osteoblasts, and finally causing OP. Recent studies revealed that microRNAs exert an essential role in regulating OS differentiation. Here, we investigated the dysregulation of miR-212 and miR-384 and the me...
Source: Experimental and Molecular Pathology - December 28, 2019 Category: Pathology Authors: Zhang Y, Jiang Y, Luo Y, Zeng Y Tags: Exp Mol Pathol Source Type: research

Association of DNA repair gene polymorphisms with colorectal cancer risk and treatment outcomes.
Abstract Colorectal cancer (CRC) is the third most common carcinoma worldwide. Despite the progress in screening and treatment, CRC remains a leading cause of cancer-related mortality. Alterations to normal nucleic acid processing may drive neoplastic transformation of colorectal epithelium. DNA repair machinery performs an essential function in the protection of genome by reducing the number of genetic polymorphisms/variations that may drive carcinogenicity. Four essential DNA repair systems are known which include nucleotide excision repair (NER), base excision repair (BER), mismatch repair (MMR), and double-str...
Source: Experimental and Molecular Pathology - December 24, 2019 Category: Pathology Authors: Al-Shaheri FN, Al-Shami KM, Gamal EH, Mahasneh AA, Ayoub NM Tags: Exp Mol Pathol Source Type: research

Alpha-syntrophin deficiency protects against non-alcoholic steatohepatitis associated increase of macrophages, CD8+ T-cells and galectin-3 in the liver.
Abstract Non-alcoholic steatohepatitis (NASH) is characterized by immune cell infiltration. Loss of the scaffold protein alpha-syntrophin (SNTA) protected mice from hepatic inflammation in the methionine-choline-deficient (MCD) diet model. Here, we determined increased numbers of macrophages and CD8+ T-cells in MCD diet induced NASH liver of wild type mice. In the mutant animals these NASH associated changes in immune cell composition were less pronounced. Further, there were more γδ T-cells in the NASH liver of the null mice. Galectin-3 protein in the hepatic non-parenchymal cell fraction was strongly...
Source: Experimental and Molecular Pathology - December 24, 2019 Category: Pathology Authors: Rein-Fischboeck L, Haberl EM, Bajraktari G, Feder S, Pohl R, Eggenhofer E, Buechler C Tags: Exp Mol Pathol Source Type: research

IGFBP-2 in cervical cancer development.
CONCLUSION: In cervical carcinogenesis, IGFBP-2 appears to play an oncogenic role, probably through an IGF-independent mechanism. PMID: 31870856 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 20, 2019 Category: Pathology Authors: Kaur G, Balasubramaniam SD, Lee YJ Tags: Exp Mol Pathol Source Type: research

Expression and methylation levels of suppressor of cytokine signaling 3 in rheumatic arthritis synovial fibroblasts.
Abstract BACKGROUND: In the present study, we aimed to understand the expression and methylation levels of the suppressor of cytokine signaling 3 (SOCS3) in rheumatoid arthritis (RA) synovial fibroblasts. METHOD: The RA model was established using Freund's complete adjuvant, and then the synovial fibroblasts were isolated and cultured. Next, RNA extraction and reverse transcription were performed. The SOCS3 transcription level was detected using qPCR, and SOCS3 protein expression was detected using western blotting (WB). Lastly, methylation-specific PCR (MSP) was performed. RESULTS: The RA model was succ...
Source: Experimental and Molecular Pathology - December 16, 2019 Category: Pathology Authors: Meng C, Qi H, Wang X, Wu X, Huang W, Wang H, He Y Tags: Exp Mol Pathol Source Type: research

Introducing a panel for early detection of lung adenocarcinoma by using data integration of genomics, epigenomics, transcriptomics and proteomics.
Abstract Lung Adenocarcinoma is one of the most leading causes of death worldwide. Early detection of this cancer could enhance the survival chance of patients and even lead to better and more effective treatment. One of the approaches to find out more about biological malfunctions is using "omics" data. Among diverse computational procedures, data integration is becoming a striking tool to deal with complicated diseases such as cancer, considering the defective and informative nature of each kind of "omics" data. Data integration as relates to lung adenocarcinoma can lead to finding molecular ...
Source: Experimental and Molecular Pathology - December 13, 2019 Category: Pathology Authors: Haghjoo N, Moeini A, Masoudi-Nejad A Tags: Exp Mol Pathol Source Type: research

LncRNA GAS5 suppresses inflammatory responses and apoptosis of alveolar epithelial cells by targeting miR-429/DUSP1.
This study firstly investigated the role and mechanism of long non-coding RNA (lncRNA) growth arrest-specific 5 (GAS5) in regulating lipopolysaccharide (LPS)-induced inflammatory response and apoptosis of murine alveolar epithelial cell line MLE-12. The expression of GAS5, miR-429, and dual-specificity phosphatase 1 (DUSP1) were examined using quantitative Real-Time PCR (qRT-PCR) and western blot. The inflammatory responses were evaluated by detecting the levels of pro-inflammatory cytokines using ELISA. Cell apoptosis was assessed by TUNEL assay. The interactions among GAS5, miR-429, and DUSP1 were examined using lucifera...
Source: Experimental and Molecular Pathology - December 11, 2019 Category: Pathology Authors: Li J, Liu S Tags: Exp Mol Pathol Source Type: research

Expression analysis of NF- κB interacting long noncoding RNAs in breast cancer.
Expression analysis of NF-κB interacting long noncoding RNAs in breast cancer. Exp Mol Pathol. 2019 Dec 11;:104359 Authors: Dashti S, Ghafouri-Fard S, Esfandi F, Oskooei VK, Arsang-Jang S, Taheri M Abstract The nuclear factor-κB (NF-κB) has a prominent role in development of breast cancer and response of patients to conventional therapies. Several factors regulate the activity of this transcription factor. In the current investigation, we compared expression levels of five long non-coding RNAs (lncRNAs) with putative interactions with NF-κB namely CHAST, ADINR, DICER1-AS1, HNF1...
Source: Experimental and Molecular Pathology - December 11, 2019 Category: Pathology Authors: Dashti S, Ghafouri-Fard S, Esfandi F, Oskooei VK, Arsang-Jang S, Taheri M Tags: Exp Mol Pathol Source Type: research

LncRNA KCNQ1OT1 contributes to oxygen-glucose-deprivation/reoxygenation-induced injury via sponging miR-9 in cultured neurons to regulate MMP8.
Abstract Our study proposed to investigate the function of potassium voltage-gated channel sub-family Q member 1 opposite strand 1 (KCNQ1OT1) in cerebral ischemia-reperfusion (I/R) injury and the underlying mechanism. We constructed an oxygen-glucose-deprivation/reoxygenation (OGD/R) model using the primary cortical neurons to mimic the cerebral I/R injury in vitro. Small inference RNA (siRNA) was used to silencing KCNQ1OT1. Dual luciferase assay was conducted to verify the interaction between KCNQ1OT1 and miR-9 and interaction between miR-9 and MMP8. CCK8 assay and flow cytometry analysis were applied for determi...
Source: Experimental and Molecular Pathology - December 11, 2019 Category: Pathology Authors: Ren Y, Gao XP, Liang H, Zhang H, Hu CY Tags: Exp Mol Pathol Source Type: research

Telomere-associated genes and telomeric lncRNAs are biomarker candidates in lung squamous cell carcinoma (LUSC).
Abstract In the past decade, research efforts were made to identify molecular biomarkers useful as therapeutic targets in Non-Small Cell Lung Cancer (NSCLC), the most frequent type of lung carcinoma. NSCLC presents different histological subtypes being the most prevalent LUSC (Lung Squamous Cell Cancer) and LUAD (Lung Adenocarcinoma), and only a subset of LUAD patients' present tumors expressing known targetable genetic alterations. Telomeres and its components, including telomerase, the enzyme that replenishes telomeres, have been considered potential cancer biomarkers due to their crucial role in cell proliferat...
Source: Experimental and Molecular Pathology - December 11, 2019 Category: Pathology Authors: Storti CB, de Oliveira RA, de Carvalho M, Hasimoto EN, Cataneo DC, Cataneo AJM, De Faveri J, Vasconcelos EJR, Dos Reis PP, Cano MIN Tags: Exp Mol Pathol Source Type: research

Notoginsenoside R1 suppresses miR-301a via NF- κB pathway in lipopolysaccharide-treated ATDC5 cells.
CONCLUSION: NG-R1 relived LPS-elicited inflammatory damages via blocking NF-κB in a miR-301a-silenced manner. PMID: 31837326 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 11, 2019 Category: Pathology Authors: Dong Y, Yan X, Yang X, Yu C, Deng Y, Song X, Zhang L Tags: Exp Mol Pathol Source Type: research

miR-130a-3p inhibition protects against renal fibrosis in vitro via the TGF- β1/Smad pathway by targeting SnoN.
CONCLUSION: Our study verified that miR-130a-3p facilitates the TGF-β1/Smad pathway in renal tubular epithelial cells and may participate in renal fibrosis by targeting SnoN, which could be a possible strategy for renal fibrosis treatment. PMID: 31836508 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 10, 2019 Category: Pathology Authors: Ai K, Zhu X, Kang Y, Li H, Zhang L Tags: Exp Mol Pathol Source Type: research

Expression and function of long non-coding RNAs in head and neck squamous cell carcinoma.
Abstract No longer regarded as junk DNA, long non-coding RNAs (lncRNAs) are considered as master regulators of cancer development and metastasis nowadays. Among the recently appreciated roles of these transcripts is their fundamental contribution in the pathogenesis of head and neck squamous cell carcinoma (HNSCC). Notably, lncRNAs may have interactions with some environmental risk factors for this type of cancer. Moreover, a number of studies have verified diagnostic and prognostic values of lncRNAs in HNSCC. Emerging evidences from functional studies point to the possibility of design of lncRNA-targeted therapie...
Source: Experimental and Molecular Pathology - December 5, 2019 Category: Pathology Authors: Ghafouri-Fard S, Mohammad-Rahimi H, Jazaeri M, Taheri M Tags: Exp Mol Pathol Source Type: research

The inhibitory effect and safety of GANT61 on HeLa cells in nude mice.
In this study, we aimed to investigate the inhibitory effect of GANT61 on cervical cancer and to study its safety in nude mice. We used in vivo experiments to assess the effect of GANT61 on the growth of cervical cancer HeLa cells, and we measured the WBC, HGB, PLT, ALT, AST and Cre levels in nude mice. Next, we examined the organ and tumor morphology and distant metastasis by HE staining. We used immunohistochemistry to monitor the expression levels of Gli1, FoxM1, Ki-67, cyclinD1, E-cadherin, vimentin, survivin, caspase-3 and CD34+. Western blotting and RT-RCR were used to measure Gli1 expression. GANT61 inhibited the gr...
Source: Experimental and Molecular Pathology - December 3, 2019 Category: Pathology Authors: Chang Y, Chen H, Duan J, Wu W, Le F, Mou F Tags: Exp Mol Pathol Source Type: research

Overexpression of cortistatin alleviates oxygen/glucose-deprivation-induced ER stress and prompts neural stem cell proliferation via SSTR2.
Abstract Cerebral infarction (CI), a blood circulatory disorder, causes a high mortality and disability rate worldwide. Intriguingly, a newly discovered neuropeptide, Cortistatin (CST), has been indicated to inhibit the cortical activity. In our research, we aimed to explore the functional relevance of CST in neural stem cells (NSCs) in CI rats. The expression of CST was determined in NSCs induced by oxygen-glucose deprivation (OGD). NSCs isolated from the embryonic rat brain were treated with OGD to establish an in vitro CI model while dithiothreitol (DTT) was introduced to induce endoplasmic reticulum stress (ER...
Source: Experimental and Molecular Pathology - December 3, 2019 Category: Pathology Authors: Liang X, Mao Q, Huang D, Tang J, Zheng J Tags: Exp Mol Pathol Source Type: research

ACE2 exhibits protective effects against LPS-induced acute lung injury in mice by inhibiting the LPS-TLR4 pathway.
In conclusion, ACE2 was expressed at low levels in response to LPS-induced ALI. Overexpression of ACE2 regulates the ACE2/Ang-(1-7)/Mas and ACE/Ang II/AT1 axes to maintain dynamic balance of the renin-angiotensin system, and attenuate inflammatory response. PMID: 31805278 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - December 2, 2019 Category: Pathology Authors: Ye R, Liu Z Tags: Exp Mol Pathol Source Type: research

Geniposide plays anti-tumor effects by down-regulation of microRNA-224 in HepG2 and Huh7 cell lines.
CONCLUSION: GEN played anti-tumor roles by targeting miR-224 via blocking the Wnt/β-catenin and AKT cascades in the HepG2 and Huh7 cells. PMID: 31778668 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 25, 2019 Category: Pathology Authors: Yu X, Wang Y, Tao S, Sun S Tags: Exp Mol Pathol Source Type: research

miR-488-5p and its role in melanoma.
This study showed the involvement of miR-488-5p in Wnt/β-catenin pathway and the function as tumor suppressor. Transfection of miR-488-5p mimic led to inhibition of proliferation, migration, anchorage independent growth and led to induction of apoptosis. These data indicated that miR-488-5p acts as a tumor suppressor and is lost during melanoma development which has influence on proliferation and migration. The loss of miR-488-5p was confirmed in vivo by in situ hybridization on melanoma tissue. PMID: 31765608 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 22, 2019 Category: Pathology Authors: Arnold J, Engelmann JC, Schneider N, Bosserhoff AK, Kuphal S Tags: Exp Mol Pathol Source Type: research

The relevance of tumor mutation profiling in interpretation of NGS data from cell-free DNA in non-small cell lung cancer patients.
In conclusion, we were able to identify tumor mutations in ctDNA from 57% of NSCLC patients using a comprehensive gene panel. We demonstrated that sequencing paired tumor DNA was helpful to interpret data and confirm ctDNA, and thus increased the ratio of patients with detectable ctDNA. This approach might be feasible for mutation analysis of ctDNA in routine diagnostic practice, especially in case of suboptimal plasma quality and quantity. PMID: 31759951 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 21, 2019 Category: Pathology Authors: Ottestad AL, Wahl SGF, Grønberg BH, Skorpen F, Dai HY Tags: Exp Mol Pathol Source Type: research

Suppression of miR-143-3p contributes to the anti-fibrosis effect of atorvastatin on myocardial tissues via the modulation of Smad2 activity.
Abstract Atorvastatin is a commonly prescribed statin drug for the control of lipid synthesis and recent studies have shown the cardiac protection potential of atorvastatin. Cardiac fibrosis is a critical process that impairs heart function. In the current study, the anti-fibrosis potential of atorvastatin was assessed and the mechanism associated with the treatment was explored. Fibrotic symptoms were induced using transverse aortic constriction (TAC) method in vivo and using TGF-β1 in vitro. The effect of atorvastatin on the development of cardiac fibrosis was firstly measured. Moreover, the influence of mi...
Source: Experimental and Molecular Pathology - November 20, 2019 Category: Pathology Authors: Yu B, Yu M, Zhang H, Xie D, Nie W, Shi K Tags: Exp Mol Pathol Source Type: research

EGFRvIII epigenetically regulates ARHI to promote glioma cell proliferation and migration.
Abstract Epidermal growth factor receptor variant III (EGFRvIII) is a tumor-specific mutation widely expressed in glioma. However, its role and molecular mechanism in glioma have not been completely elucidated. Immunohistochemistry analyses of EGFRvIII, enhancer of zeste homolog 2 (EZH2) and aplysia ras homolog I (ARHI) were performed in tumor tissues from patients with glioma. Regulatory mechanisms among EGFRvIII, EZH2 and ARHI were examined by western blot and chromatin immunoprecipitation (ChIP). Cell proliferation and migration of glioma cells were examined. EGFRvIII and EZH2 expression were upregulated, while...
Source: Experimental and Molecular Pathology - November 18, 2019 Category: Pathology Authors: Zheng Y, Ma Y, Yue H, Liu G, Han S Tags: Exp Mol Pathol Source Type: research

KIR2DS4, KIR2DL2, and KIR2DS4del are linked with basaloid tumors, lymph node metastasis, advanced stage and metastatic risk in head and neck squamous cell carcinoma.
CONCLUSION: In HNSCC, KIR2DL2 is positively while KIR2DS4 is negatively associated with advanced stage. The higher proportion of LNM in carriers of KIR2DL2 and carriers of 5 iKIRs, suggested that inhibitory KIRs are associated with metastatic risk. PMID: 31751561 [PubMed - as supplied by publisher] (Source: Experimental and Molecular Pathology)
Source: Experimental and Molecular Pathology - November 18, 2019 Category: Pathology Authors: Barani S, Khademi B, Ghaderi A Tags: Exp Mol Pathol Source Type: research