Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides.

Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides.
Adv Exp Med Biol. 2019;1140:265-287
Authors: Mihăşan M, Wormwood KL, Sokolowska I, Roy U, Woods AG, Darie CC
Abstract
Recent developments of mass spectrometry (MS) allow us to identify, estimate, and characterize proteins and protein complexes. At the same time, structural biology helps to determine the protein structure and its structure-function relationship. Together, they aid to understand the protein structure, property, function, protein-complex assembly, protein-protein interaction, and dynamics. The present chapter is organized with illustrative results to demonstrate how experimental mass spectrometry can be combined with computational structural biology for detailed studies of protein's structures. We have used tumor differentiation factor protein/peptide as ligand and Hsp70/Hsp90 as receptor protein as examples to study ligand-protein interaction. To investigate possible protein conformation, we will describe two proteins-lysozyme and myoglobin. As an application of MS-based assignment of disulfide bridges, the case of the spider venom polypeptide Phα1β will also be discussed.
PMID: 31347053 [PubMed - in process]

Source: Advances in Experimental Medicine and Biology - July 29, 2019 Category: Research Tags: Adv Exp Med Biol Source Type: research