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Specialty: Biotechnology

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Total 393 results found since Jan 2013.

Naringenin exerts cytoprotective effect against Paraquat-induced toxicity in Human Bronchial Epithelial BEAS-2B cells through NRF2 activation.
In this study, we intended to assess the cytoprotective effect of NG against PQ-induced toxicity in human bronchial epithelial BEAS-2B cell line. Co-treatment with NG in PQ-treated BEAS-2B cells can reduce PQ-induced cellular toxicity. NG can also decrease the generation of intracellular ROS caused by PQ treatment. We also observed that treatment with NG in PQ-exposed BEAS-2B cells can significantly induce the expression of antioxidant-related genes, including GPX2, GPX3, GPX5, and GPX7. NG co-treatment can also activate the NRF2 transcription factor and promote its nuclear translocation. In addition, NG co-treatment can i...
Source: Journal of Microbiology and Biotechnology - February 25, 2014 Category: Biotechnology Authors: Podder B, Song HY, Kim YS Tags: J Microbiol Biotechnol Source Type: research

HMGB1 enhances embryonic neural stem cell proliferation by activating the MAPK signaling pathway.
Abstract Neural stem cells (NSCs) are involved in neural tube formation. As the high-mobility group box 1 (HMGB1) protein is involved in neurulation and is present at elevated levels in neural tube defects (NTDs) induced by hyperthermia, we have now investigated the effects of HMGB1 on proliferation, differentiation, and MAPK signaling pathways of NSCs in vitro. We constructed a lentivirus vector with HMGB1 siRNA and used it to infect NSCs. Down-regulation of HMGB1 expression was confirmed. Proliferation of NSCs was determined by MTS and nestin/BrdU double-labeling. Differentiation of NSCs was assessed using β-tu...
Source: Biotechnology Letters - April 19, 2014 Category: Biotechnology Authors: Wang L, Yu L, Zhang T, Wang L, Leng Z, Guan Y, Wang X Tags: Biotechnol Lett Source Type: research

miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2.
Abstract MicroRNAs play important roles in carcinogenesis by negatively regulating the expression of target genes. Here we explore the biological function of miR-155 and the underlying mechanism in colorectal carcinoma. We validate, for the first time, that E2F2 is a direct target of miR-155 using western blot and a luciferase reporter assay and that miR-155 regulates the proliferation and cell cycle of colorectal carcinoma cells by targeting E2F2 using siRNA technology. We also found, for the first, time that E2F2 acts as a tumor suppressor in colorectal carcinoma. Overall, miR-155 plays an important role in colo...
Source: Biotechnology Letters - May 4, 2014 Category: Biotechnology Authors: Li T, Yang J, Lv X, Liu K, Gao C, Xing Y, Xi T Tags: Biotechnol Lett Source Type: research

Tumor suppressor protein p53 promotes 2-methoxyestradiol-induced activation of Bak and Bax, leading to mitochondria-dependent apoptosis in human colon cancer HCT116 cells.
Abstract To examine the effect of tumor suppressor protein p53 on the antitumor activity of 2-methoxyestradiol (2-MeO-E2), 2-MeO-E2-induced cell cycle changes and apoptotic events were compared between human colon carcinoma cell lines HCT116 (p53+/+) and HCT116 (p53-/-). When both cell types were exposed to 2-MeO-E2, a reduction in the cell viability and an enhancement in the proportions of G2/M cells and apoptotic sub-G1 cells commonly occurred dose-dependently. These 2-MeO-E2-induced cellular changes, except for G2/M arrest, appeared to be more apparent in the presence of p53. Immunofluorescence microscopic anal...
Source: Journal of Microbiology and Biotechnology - September 1, 2014 Category: Biotechnology Authors: Lee JY, Lee SB, Park WY, Choi YJ, Kim B, Kim YH, Jun DY, Kim YH Tags: J Microbiol Biotechnol Source Type: research

Transgenerational inheritance of epigenetic response to cold in Arabidopsis thaliana
Publication date: Available online 8 September 2014 Source:Biocatalysis and Agricultural Biotechnology Author(s): Zoë Migicovsky , Igor Kovalchuk Exposure to stresses such as temperature fluctuations, drought and UV can cause physiological and epigenetic changes in plants. Such changes may be inherited by progeny of stressed plants and may alter their response to stress. To understand the ability of plants to inherit an epigenetic memory of exposure to cold stress and to analyze physiological manifestations of this memory, we propagated cold-stressed and control plants and compared their progeny under both normal and st...
Source: Biocatalysis and Agricultural Biotechnology - October 17, 2014 Category: Biotechnology Source Type: research

Overexpression of Serpinb1 in Chinese Hamster Ovary Cells Increases Recombinant IgG Productivity
We report the discovery and validation of a novel CHO cell engineering target for improving IgG expression, serpin peptidase inhibitor, clade B, member 1 (Serpinb1). Transcriptomic studies using microarrays revealed that Serpinb1 was up-regulated in cultures with IgG heavy and light chain transcription transiently repressed compared with cultures treated with non-targeting siRNA. As proof of concept, a lentiviral vector was employed to overexpress the Chinese Hamster Serpinb1 in a CHOZN® Glutamine Synthetase (-/-) recombinant IgG producing CHO line. The lentiviral stable pool demonstrated 4.2-fold SERPINB1 overexpression ...
Source: Journal of Biotechnology - November 14, 2014 Category: Biotechnology Source Type: research

Gene silencing effect of SiRNA-magnetic modified with biodegradable copolymer nanoparticles on hTERT gene expression in lung cancer cell line
Artificial Cells, Nanomedicine, and Biotechnology, Ahead of Print.
Source: Artificial Cells, Blood Substitutes, and Biotechnology - July 14, 2014 Category: Biotechnology Tags: article Source Type: research

Preparation and evaluation of multifunctional envelope-type nano device loading siRNA
Artificial Cells, Nanomedicine, and Biotechnology, Ahead of Print.
Source: Artificial Cells, Blood Substitutes, and Biotechnology - May 16, 2014 Category: Biotechnology Tags: article Source Type: research

Efficient delivery of RNAi prodrugs containing reversible charge-neutralizing phosphotriester backbone modifications
Nature Biotechnology 32, 1256 (2014). doi:10.1038/nbt.3078 Authors: Bryan R Meade, Khirud Gogoi, Alexander S Hamil, Caroline Palm-Apergi, Arjen van den Berg, Jonathan C Hagopian, Aaron D Springer, Akiko Eguchi, Apollo D Kacsinta, Connor F Dowdy, Asaf Presente, Peter Lönn, Manuel Kaulich, Naohisa Yoshioka, Edwige Gros, Xian-Shu Cui & Steven F Dowdy RNA interference (RNAi) has great potential to treat human disease. However, in vivo delivery of short interfering RNAs (siRNAs), which are negatively charged double-stranded RNA macromolecules, remains a major hurdle. Current siRNA delivery has begun to move awa...
Source: Nature Biotechnology - November 17, 2014 Category: Biotechnology Authors: Bryan R MeadeKhirud GogoiAlexander S HamilCaroline Palm-ApergiArjen van den BergJonathan C HagopianAaron D SpringerAkiko EguchiApollo D KacsintaConnor F DowdyAsaf PresentePeter LönnManuel KaulichNaohisa YoshiokaEdwige GrosXian-Shu CuiSteven F Dowdy Tags: Research Source Type: research

Effects of chitosan nanoparticle-mediated BRAF siRNA interference on invasion and metastasis of gastric cancer cells
Artificial Cells, Nanomedicine, and Biotechnology, Ahead of Print.
Source: Artificial Cells, Blood Substitutes, and Biotechnology - March 22, 2015 Category: Biotechnology Tags: article Source Type: research

Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device
Artificial Cells, Nanomedicine, and Biotechnology, Ahead of Print.
Source: Artificial Cells, Blood Substitutes, and Biotechnology - March 27, 2015 Category: Biotechnology Tags: article Source Type: research

Targeting mitosis-regulating genes in cisplatin-sensitive and -resistant melanoma cells: A live-cell RNAi screen displays differential nucleus-derived phenotypes.
Abstract Chemoresistance in malignant melanoma remains an unresolved clinical issue. In the search for novel molecular targets a live-cell high-content RNAi screen based on gene expression data was performed in cisplatin-sensitive and cisplatin-resistant MeWo melanoma cells, Mel-28 cells and a melanocyte cell line. Cells were exposed to 91 siRNAs and distinct nucleus-derived phenotypes such as cell division, cell death and migration phenotypes were detected by time-lapse microscopy over 60 hours. Using this approach, cisplatin-sensitive and cisplatin-resistant melanoma cells were compared by automated image analys...
Source: Biotechnology Journal - April 16, 2015 Category: Biotechnology Authors: Erfle H, Pashayeva K, Harder N, Zhang L, Rohr K, Schadendorf D, Ugurel S, Keese M Tags: Biotechnol J Source Type: research

Using RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Prokaryotic and Eukaryotic Cells
RNA nanotechnology is an emerging field at the interface of biochemistry and nanomaterials that shows immense promise for applications in nanomedicines, therapeutics and nanotechnology. Noncoding RNAs, such as siRNA, miRNA, ribozymes, and riboswitches, play important roles in the regulation of cellular processes. They carry out highly specific functions on a compact and efficient footprint. The properties of specificity and small size make them excellent modules in the construction of multifaceted RNA nanoparticles for targeted delivery and therapy. Biological activity of RNA molecules, however, relies on their proper fold...
Source: Springer protocols feed by Biotechnology - April 25, 2015 Category: Biotechnology Source Type: news

Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor
Cumulative progress in nanoparticle development has opened a new era of targeted delivery of therapeutics to cancer cells and tissue. However, developing proper detection methods has lagged behind resulting in the lack of precise evaluation and monitoring of the systemically administered nanoparticles. RNA nanoparticles derived from the bacteriophage phi29 DNA packaging motor pRNA have emerged as a new generation of drugs for cancer therapy. Multifunctional RNA nanoparticles can be fabricated by bottom-up self-assembly of engineered RNA fragments harboring targeting (RNA aptamer or chemical ligand), therapeutic (siRNA, miR...
Source: Springer protocols feed by Biotechnology - April 25, 2015 Category: Biotechnology Source Type: news

Achieving successful delivery of oligonucleotides - from physico-chemical characterization to in vivo evaluation.
Abstract RNA interference is one of the most promising fields in modern medicine to treat several diseases, ranging from cancer to cardiac diseases, passing through viral infections and metabolic pathologies. Since the discovery of the potential therapeutic properties of non-self oligonucleotides, it was clear that it is important to develop delivery systems that are able to increase plasma stability and bestow membrane-crossing abilities to the oligonucleotides in order to reach their cytoplasmic targets. Polymer therapeutics, among other systems, are widely investigated as delivery systems for therapeutic agents...
Source: Biotechnology Advances - April 24, 2015 Category: Biotechnology Authors: Scomparin A, Polyak D, Krivitsky A, Satchi-Fainaro R Tags: Biotechnol Adv Source Type: research