Aptamer-modified paper-based analytical devices for the detection of food hazards: Emerging applications and future perspective
Biotechnol Adv. 2024 Apr 29:108368. doi: 10.1016/j.biotechadv.2024.108368. Online ahead of print.ABSTRACTFood analysis plays a critical role in assessing human health risks and monitoring food quality and safety. Currently, there is a pressing need for a reliable, portable, and quick recognition element for point-of-care testing (POCT) to better serve the demands of on-site food analysis. Aptamer-modified paper-based analytical devices (Apt-PADs) have excellent characteristics of high portability, high sensitivity, high specificity, and on-site detection, which have been widely used and concerned in the field of food safet...
Source: Biotechnology Advances - May 1, 2024 Category: Biotechnology Authors: Mingwei Qin Imran Mahmood Khan Ning Ding Shuo Qi Xiaoze Dong Yin Zhang Zhouping Wang Source Type: research
Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms
Biotechnol Adv. 2024 Apr 29;73:108370. doi: 10.1016/j.biotechadv.2024.108370. Online ahead of print.ABSTRACTRecombinant adeno-associated viruses (rAAVs) stand at the forefront of gene therapy applications, holding immense significance for their safe and efficient gene delivery capabilities. The constantly increasing and unmet demand for rAAVs underscores the need for a more comprehensive understanding of AAV biology and its impact on rAAV production. In this literature review, we delved into AAV biology and rAAV manufacturing bioprocesses, unravelling the functions and essentiality of proteins involved in rAAV production. ...
Source: Biotechnology Advances - May 1, 2024 Category: Biotechnology Authors: David Catal án-Tatjer Konstantina Tzimou Lars K Nielsen Jes ús Lavado-García Source Type: research
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications
Biotechnol Adv. 2024 Apr 27:108369. doi: 10.1016/j.biotechadv.2024.108369. Online ahead of print.ABSTRACTElectromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO2 into methane. Unlike biomethanation processes where CO2 is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines electrodes and microorganisms. Thereby, mixed, or pure methanogenic cultures catalyze the reduction of CO2 to methane v...
Source: Biotechnology Advances - April 30, 2024 Category: Biotechnology Authors: Paola Andrea Palacios Jo Philips Anders Bentien Michael Vedel Wegener Kofoed Source Type: research
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications
Biotechnol Adv. 2024 Apr 27:108369. doi: 10.1016/j.biotechadv.2024.108369. Online ahead of print.ABSTRACTElectromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO2 into methane. Unlike biomethanation processes where CO2 is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines electrodes and microorganisms. Thereby, mixed, or pure methanogenic cultures catalyze the reduction of CO2 to methane v...
Source: Biotechnology Advances - April 30, 2024 Category: Biotechnology Authors: Paola Andrea Palacios Jo Philips Anders Bentien Michael Vedel Wegener Kofoed Source Type: research
Relevance of extracellular electron uptake mechanisms for electromethanogenesis applications
Biotechnol Adv. 2024 Apr 27:108369. doi: 10.1016/j.biotechadv.2024.108369. Online ahead of print.ABSTRACTElectromethanogenesis has emerged as a biological branch of Power-to-X technologies that implements methanogenic microorganisms, as an alternative to chemical Power-to-X, to convert electrical power from renewable sources, and CO2 into methane. Unlike biomethanation processes where CO2 is converted via exogenously added hydrogen, electromethanogenesis occurs in a bioelectrochemical set-up that combines electrodes and microorganisms. Thereby, mixed, or pure methanogenic cultures catalyze the reduction of CO2 to methane v...
Source: Biotechnology Advances - April 30, 2024 Category: Biotechnology Authors: Paola Andrea Palacios Jo Philips Anders Bentien Michael Vedel Wegener Kofoed Source Type: research
Sustainable media feedstocks for cellular agriculture
Biotechnol Adv. 2024 Apr 26:108367. doi: 10.1016/j.biotechadv.2024.108367. Online ahead of print.ABSTRACTThe global food system is shifting towards cellular agriculture, a second domestication marked by cultivating microorganisms and tissues for sustainable food production. This involves tissue engineering, precision fermentation, and microbial biomass fermentation to establish food value chains independent of traditional agriculture. However, these techniques rely on growth media sourced from agricultural, chemical (fossil fuels), and mining supply chains, raising concerns about land use competition, emissions, and resour...
Source: Biotechnology Advances - April 28, 2024 Category: Biotechnology Authors: Lutz Grossmann Source Type: research
Sustainable media feedstocks for cellular agriculture
Biotechnol Adv. 2024 Apr 26:108367. doi: 10.1016/j.biotechadv.2024.108367. Online ahead of print.ABSTRACTThe global food system is shifting towards cellular agriculture, a second domestication marked by cultivating microorganisms and tissues for sustainable food production. This involves tissue engineering, precision fermentation, and microbial biomass fermentation to establish food value chains independent of traditional agriculture. However, these techniques rely on growth media sourced from agricultural, chemical (fossil fuels), and mining supply chains, raising concerns about land use competition, emissions, and resour...
Source: Biotechnology Advances - April 28, 2024 Category: Biotechnology Authors: Lutz Grossmann Source Type: research
Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes
Biotechnol Adv. 2024 Apr 25:108365. doi: 10.1016/j.biotechadv.2024.108365. Online ahead of print.ABSTRACTCarbohydrate binding modules (CBMs) are independent non-catalytic domains widely found in carbohydrate-active enzymes (CAZymes), and they play an essential role in the substrate binding process of CAZymes by guiding the appended catalytic modules to the target substrates. Owing to their precise recognition and selective affinity for different substrates, CBMs have received increasing research attention over the past few decades. To date, CBMs from different origins have formed a large number of families that show a vari...
Source: Biotechnology Advances - April 27, 2024 Category: Biotechnology Authors: Yuxian You Haocun Kong Caiming Li Zhengbiao Gu Xiaofeng Ban Zhaofeng Li Source Type: research
Protein engineering for the synthetic cell factory: Recent advance and perspective
Biotechnol Adv. 2024 Apr 23:108366. doi: 10.1016/j.biotechadv.2024.108366. Online ahead of print.ABSTRACTSynthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles. Protein engineering is a technology for creating novel protein variants with desired properties and functions by modify...
Source: Biotechnology Advances - April 25, 2024 Category: Biotechnology Authors: Ailin Guan Zixi He Xin Wang Zhi-Jun Jia Jiufu Qin Source Type: research
Protein engineering for the synthetic cell factory: Recent advance and perspective
Biotechnol Adv. 2024 Apr 23:108366. doi: 10.1016/j.biotechadv.2024.108366. Online ahead of print.ABSTRACTSynthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles. Protein engineering is a technology for creating novel protein variants with desired properties and functions by modify...
Source: Biotechnology Advances - April 25, 2024 Category: Biotechnology Authors: Ailin Guan Zixi He Xin Wang Zhi-Jun Jia Jiufu Qin Source Type: research
Protein engineering for the synthetic cell factory: Recent advance and perspective
Biotechnol Adv. 2024 Apr 23:108366. doi: 10.1016/j.biotechadv.2024.108366. Online ahead of print.ABSTRACTSynthetic cell factory offers substantial advantages in economically efficient production of biofuels, chemicals, and pharmaceutical compounds. However, to create a high-performance synthetic cell factory, precise regulation of cellular material and energy flux is essential. In this context, protein components including enzymes, transcription factor-based biosensors and transporters play pivotal roles. Protein engineering is a technology for creating novel protein variants with desired properties and functions by modify...
Source: Biotechnology Advances - April 25, 2024 Category: Biotechnology Authors: Ailin Guan Zixi He Xin Wang Zhi-Jun Jia Jiufu Qin Source Type: research
Biobased short chain fatty acid production - Exploring microbial community dynamics and metabolic networks through kinetic and microbial modeling approaches
Biotechnol Adv. 2024 Apr 22;73:108363. doi: 10.1016/j.biotechadv.2024.108363. Online ahead of print.ABSTRACTIn recent years, there has been growing interest in harnessing anaerobic digestion technology for resource recovery from waste streams. This approach has evolved beyond its traditional role in energy generation to encompass the production of valuable carboxylic acids, especially volatile fatty acids (VFAs) like acetic acid, propionic acid, and butyric acid. VFAs hold great potential for various industries and biobased applications due to their versatile properties. Despite increasing global demand, over 90% of VFAs a...
Source: Biotechnology Advances - April 24, 2024 Category: Biotechnology Authors: Merve Atasoy William T Scott Alberte Regueira Miguel Mauricio-Iglesias Peter J Schaap Hauke Smidt Source Type: research
Optimization strategies for CO < sub > 2 < /sub > biological fixation
This article reviews recent advances in optimizing CO2 biological fixation and intends to provide new insights into achieving efficient biological utilization of CO2. It first outlines the thermodynamic characteristics of diverse carbon fixation reactions and proposes optimization directions for CO2 biological fixation. A comprehensive overview of the catalytic mechanisms, optimization strategies, and challenges encountered by common carbon-fixing enzymes is then provided. Subsequently, potential routes for improving the efficiency of biological carbon fixation are discussed, including the ATP supply, reducing power supply...
Source: Biotechnology Advances - April 20, 2024 Category: Biotechnology Authors: Xiutao Liu Linqing Li Guang Zhao Peng Xiong Source Type: research
Optimization strategies for CO < sub > 2 < /sub > biological fixation
This article reviews recent advances in optimizing CO2 biological fixation and intends to provide new insights into achieving efficient biological utilization of CO2. It first outlines the thermodynamic characteristics of diverse carbon fixation reactions and proposes optimization directions for CO2 biological fixation. A comprehensive overview of the catalytic mechanisms, optimization strategies, and challenges encountered by common carbon-fixing enzymes is then provided. Subsequently, potential routes for improving the efficiency of biological carbon fixation are discussed, including the ATP supply, reducing power supply...
Source: Biotechnology Advances - April 20, 2024 Category: Biotechnology Authors: Xiutao Liu Linqing Li Guang Zhao Peng Xiong Source Type: research
The dawning era of oral thin films for nutraceutical delivery: From laboratory to clinic
Biotechnol Adv. 2024 Apr 12;73:108362. doi: 10.1016/j.biotechadv.2024.108362. Online ahead of print.ABSTRACTOral thin films (OTFs) are innovative dosage forms that have gained tremendous attention for the delivery of nutraceuticals. They are ultra-thin, flexible sheets that can be easily placed on the tongue, sublingual or buccal mucosa (inner lining of the cheek). These thin films possess several advantages for nutraceutical delivery including ease of administration, rapid disintegration, fast absorption, rapid onset of action, bypass first-pass hepatic metabolism, accurate dosing, enhanced stability, portability, discree...
Source: Biotechnology Advances - April 14, 2024 Category: Biotechnology Authors: None Ruchika Nabab Khan Shagun Sanjivv Dogra Ankit Saneja Source Type: research
