The role of orphan crops in the transition to nutritional quality-oriented crop improvement
Biotechnol Adv. 2023 Aug 26:108242. doi: 10.1016/j.biotechadv.2023.108242. Online ahead of print.ABSTRACTMicronutrient malnutrition is a persisting problem threatening global human health. Biofortification via metabolic engineering has been proposed as a cost-effective and short-term means to alleviate this burden. There has been a recent rise in the recognition of potential that underutilized, orphan crops can hold in decreasing malnutrition concerns. Here, we illustrate how orphan crops can serve as a medium to provide micronutrients to populations in need, whilst promoting and maintaining dietary diversity. We provide a...
Source: Biotechnology Advances - August 28, 2023 Category: Biotechnology Authors: Vincent Verbeecke Laura Cust ódio Simon Strobbe Dominique Van Der Straeten Source Type: research
The role of orphan crops in the transition to nutritional quality-oriented crop improvement
Biotechnol Adv. 2023 Aug 26:108242. doi: 10.1016/j.biotechadv.2023.108242. Online ahead of print.ABSTRACTMicronutrient malnutrition is a persisting problem threatening global human health. Biofortification via metabolic engineering has been proposed as a cost-effective and short-term means to alleviate this burden. There has been a recent rise in the recognition of potential that underutilized, orphan crops can hold in decreasing malnutrition concerns. Here, we illustrate how orphan crops can serve as a medium to provide micronutrients to populations in need, whilst promoting and maintaining dietary diversity. We provide a...
Source: Biotechnology Advances - August 28, 2023 Category: Biotechnology Authors: Vincent Verbeecke Laura Cust ódio Simon Strobbe Dominique Van Der Straeten Source Type: research
Endogenous CRISPR-Cas mediated in situ genome editing: State-of-the-art and the road ahead for engineering prokaryotes
Biotechnol Adv. 2023 Aug 24:108241. doi: 10.1016/j.biotechadv.2023.108241. Online ahead of print.ABSTRACTThe CRISPR-Cas systems have shown tremendous promise as heterologous tools for genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and the inherent toxicity of Cas9/12a proteins could easily lead to cell death, which led to the development of endogenous CRISPR-Cas systems. Programming the widespread endogenous CRISPR-Cas systems for in situ genome editing represents a promising tool in prokaryotes, especially in genetically intractable species. Here, this review briefly summarizes the adv...
Source: Biotechnology Advances - August 26, 2023 Category: Biotechnology Authors: Zhenlei Liu Jiayu Liu Zhihan Yang Liying Zhu Zhengming Zhu He Huang Ling Jiang Source Type: research
Endogenous CRISPR-Cas mediated in situ genome editing: State-of-the-art and the road ahead for engineering prokaryotes
Biotechnol Adv. 2023 Aug 24:108241. doi: 10.1016/j.biotechadv.2023.108241. Online ahead of print.ABSTRACTThe CRISPR-Cas systems have shown tremendous promise as heterologous tools for genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and the inherent toxicity of Cas9/12a proteins could easily lead to cell death, which led to the development of endogenous CRISPR-Cas systems. Programming the widespread endogenous CRISPR-Cas systems for in situ genome editing represents a promising tool in prokaryotes, especially in genetically intractable species. Here, this review briefly summarizes the adv...
Source: Biotechnology Advances - August 26, 2023 Category: Biotechnology Authors: Zhenlei Liu Jiayu Liu Zhihan Yang Liying Zhu Zhengming Zhu He Huang Ling Jiang Source Type: research
Synthetic biology tools for environmental protection
Biotechnol Adv. 2023 Aug 22:108239. doi: 10.1016/j.biotechadv.2023.108239. Online ahead of print.ABSTRACTSynthetic biology transforms the way we perceive biological systems. Emerging technologies in this field affect many disciplines of science and engineering. Traditionally, synthetic biology approaches were commonly aimed at developing cost-effective microbial cell factories to produce chemicals from renewable sources. Based on this, the immediate beneficial impact of synthetic biology on the environment came from reducing our oil dependency. However, synthetic biology is starting to play a more direct role in environmen...
Source: Biotechnology Advances - August 24, 2023 Category: Biotechnology Authors: Javad Aminian-Dehkordi Shadi Rahimi Mehdi Golzar-Ahmadi Amritpal Singh Javiera Lopez Rodrigo Ledesma-Amaro Ivan Mijakovic Source Type: research
Growth-coupled high throughput selection for directed enzyme evolution
Biotechnol Adv. 2023 Aug 22:108238. doi: 10.1016/j.biotechadv.2023.108238. Online ahead of print.ABSTRACTDirected enzyme evolution has revolutionized the rapid development of enzymes with desired properties. However, the lack of a high-throughput method to identify the most suitable variants from a large pool of genetic diversity poses a major bottleneck. To overcome this challenge, growth-coupled in vivo high-throughput selection approaches (GCHTS) have emerged as a novel selection system for enzyme evolution. GCHTS links the survival of the host cell with the properties of the target protein, resulting in a screening sys...
Source: Biotechnology Advances - August 24, 2023 Category: Biotechnology Authors: Zhengqun Li Yuting Deng Guang-Yu Yang Source Type: research
Synthetic biology tools for environmental protection
Biotechnol Adv. 2023 Aug 22:108239. doi: 10.1016/j.biotechadv.2023.108239. Online ahead of print.ABSTRACTSynthetic biology transforms the way we perceive biological systems. Emerging technologies in this field affect many disciplines of science and engineering. Traditionally, synthetic biology approaches were commonly aimed at developing cost-effective microbial cell factories to produce chemicals from renewable sources. Based on this, the immediate beneficial impact of synthetic biology on the environment came from reducing our oil dependency. However, synthetic biology is starting to play a more direct role in environmen...
Source: Biotechnology Advances - August 24, 2023 Category: Biotechnology Authors: Javad Aminian-Dehkordi Shadi Rahimi Mehdi Golzar-Ahmadi Amritpal Singh Javiera Lopez Rodrigo Ledesma-Amaro Ivan Mijakovic Source Type: research
Growth-coupled high throughput selection for directed enzyme evolution
Biotechnol Adv. 2023 Aug 22:108238. doi: 10.1016/j.biotechadv.2023.108238. Online ahead of print.ABSTRACTDirected enzyme evolution has revolutionized the rapid development of enzymes with desired properties. However, the lack of a high-throughput method to identify the most suitable variants from a large pool of genetic diversity poses a major bottleneck. To overcome this challenge, growth-coupled in vivo high-throughput selection approaches (GCHTS) have emerged as a novel selection system for enzyme evolution. GCHTS links the survival of the host cell with the properties of the target protein, resulting in a screening sys...
Source: Biotechnology Advances - August 24, 2023 Category: Biotechnology Authors: Zhengqun Li Yuting Deng Guang-Yu Yang Source Type: research
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0
Biotechnol Adv. 2023 Aug 19:108237. doi: 10.1016/j.biotechadv.2023.108237. Online ahead of print.ABSTRACTThe evolution of industries has introduced versatile technologies, motivating limitless possibilities of tackling pivotal global predicaments in the arenas of medicine, environment, defence, and national security. In this direction, ardently emerges the new era of Industry 5.0 in the eyes of biomanufacturing, which integrates the most advanced systems 21st century has to offer by means of integrating artificial systems to mimic and nativize the natural milieu to substitute the deficits of nature, thence leading to a new...
Source: Biotechnology Advances - August 21, 2023 Category: Biotechnology Authors: Abhyavartin Selvam Tanishka Aggarwal Monalisa Mukherjee Yogesh Kumar Verma Source Type: research
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0
Biotechnol Adv. 2023 Aug 19:108237. doi: 10.1016/j.biotechadv.2023.108237. Online ahead of print.ABSTRACTThe evolution of industries has introduced versatile technologies, motivating limitless possibilities of tackling pivotal global predicaments in the arenas of medicine, environment, defence, and national security. In this direction, ardently emerges the new era of Industry 5.0 in the eyes of biomanufacturing, which integrates the most advanced systems 21st century has to offer by means of integrating artificial systems to mimic and nativize the natural milieu to substitute the deficits of nature, thence leading to a new...
Source: Biotechnology Advances - August 21, 2023 Category: Biotechnology Authors: Abhyavartin Selvam Tanishka Aggarwal Monalisa Mukherjee Yogesh Kumar Verma Source Type: research
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0
Biotechnol Adv. 2023 Aug 19:108237. doi: 10.1016/j.biotechadv.2023.108237. Online ahead of print.ABSTRACTThe evolution of industries has introduced versatile technologies, motivating limitless possibilities of tackling pivotal global predicaments in the arenas of medicine, environment, defence, and national security. In this direction, ardently emerges the new era of Industry 5.0 in the eyes of biomanufacturing, which integrates the most advanced systems 21st century has to offer by means of integrating artificial systems to mimic and nativize the natural milieu to substitute the deficits of nature, thence leading to a new...
Source: Biotechnology Advances - August 21, 2023 Category: Biotechnology Authors: Abhyavartin Selvam Tanishka Aggarwal Monalisa Mukherjee Yogesh Kumar Verma Source Type: research
Humans and robots: Friends of the future? A bird's eye view of biomanufacturing industry 5.0
Biotechnol Adv. 2023 Aug 19:108237. doi: 10.1016/j.biotechadv.2023.108237. Online ahead of print.ABSTRACTThe evolution of industries has introduced versatile technologies, motivating limitless possibilities of tackling pivotal global predicaments in the arenas of medicine, environment, defence, and national security. In this direction, ardently emerges the new era of Industry 5.0 in the eyes of biomanufacturing, which integrates the most advanced systems 21st century has to offer by means of integrating artificial systems to mimic and nativize the natural milieu to substitute the deficits of nature, thence leading to a new...
Source: Biotechnology Advances - August 21, 2023 Category: Biotechnology Authors: Abhyavartin Selvam Tanishka Aggarwal Monalisa Mukherjee Yogesh Kumar Verma Source Type: research
Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae
Biotechnol Adv. 2023 Aug 14:108236. doi: 10.1016/j.biotechadv.2023.108236. Online ahead of print.ABSTRACTMicroalgae are microorganisms capable of producing bioactive compounds using photosynthesis. Microalgae contain a variety of high value-added natural pigments such as carotenoids, phycobilins, and chlorophylls. These pigments play an important role in many areas such as food, pharmaceuticals, and cosmetics. Natural pigments have a health value that is unmatched by synthetic pigments. However, the current commercial production of natural pigments from microalgae is not able to meet the growing market demand. The use of m...
Source: Biotechnology Advances - August 16, 2023 Category: Biotechnology Authors: Kai Cao Yunlin Cui Fengjie Sun Hao Zhang Jianhua Fan Baosheng Ge Yujiao Cao Xiaodong Wang Xiangyu Zhu Zuoxi Wei Qingshou Yao Jinju Ma Yu Wang Chunxiao Meng Zhengquan Gao Source Type: research
Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae
Biotechnol Adv. 2023 Aug 14:108236. doi: 10.1016/j.biotechadv.2023.108236. Online ahead of print.ABSTRACTMicroalgae are microorganisms capable of producing bioactive compounds using photosynthesis. Microalgae contain a variety of high value-added natural pigments such as carotenoids, phycobilins, and chlorophylls. These pigments play an important role in many areas such as food, pharmaceuticals, and cosmetics. Natural pigments have a health value that is unmatched by synthetic pigments. However, the current commercial production of natural pigments from microalgae is not able to meet the growing market demand. The use of m...
Source: Biotechnology Advances - August 16, 2023 Category: Biotechnology Authors: Kai Cao Yunlin Cui Fengjie Sun Hao Zhang Jianhua Fan Baosheng Ge Yujiao Cao Xiaodong Wang Xiangyu Zhu Zuoxi Wei Qingshou Yao Jinju Ma Yu Wang Chunxiao Meng Zhengquan Gao Source Type: research
Metabolic engineering and synthetic biology strategies for producing high-value natural pigments in Microalgae
Biotechnol Adv. 2023 Aug 14:108236. doi: 10.1016/j.biotechadv.2023.108236. Online ahead of print.ABSTRACTMicroalgae are microorganisms capable of producing bioactive compounds using photosynthesis. Microalgae contain a variety of high value-added natural pigments such as carotenoids, phycobilins, and chlorophylls. These pigments play an important role in many areas such as food, pharmaceuticals, and cosmetics. Natural pigments have a health value that is unmatched by synthetic pigments. However, the current commercial production of natural pigments from microalgae is not able to meet the growing market demand. The use of m...
Source: Biotechnology Advances - August 16, 2023 Category: Biotechnology Authors: Kai Cao Yunlin Cui Fengjie Sun Hao Zhang Jianhua Fan Baosheng Ge Yujiao Cao Xiaodong Wang Xiangyu Zhu Zuoxi Wei Qingshou Yao Jinju Ma Yu Wang Chunxiao Meng Zhengquan Gao Source Type: research
