Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 9;69:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenot...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 9;69:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenot...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 9;69:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenot...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 8:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenotype...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 8:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenotype...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 8:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenotype...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Biosensor-guided discovery and engineering of metabolic enzymes
Biotechnol Adv. 2023 Sep 8:108251. doi: 10.1016/j.biotechadv.2023.108251. Online ahead of print.ABSTRACTA variety of chemicals have been produced through metabolic engineering approaches, and enhancing biosynthesis performance can be achieved by using enzymes with high catalytic efficiency. Accordingly, a number of efforts have been made to discover enzymes in nature for various applications. In addition, enzyme engineering approaches have been attempted to suit specific industrial purposes. However, a significant challenge in enzyme discovery and engineering is the efficient screening of enzymes with the desired phenotype...
Source: Biotechnology Advances - September 10, 2023 Category: Biotechnology Authors: Hyun Gyu Hwang Dae-Yeol Ye Gyoo Yeol Jung Source Type: research
Design strategies for positively charged endolysins: Insights into Artilysin development
Biotechnol Adv. 2023 Sep 5:108250. doi: 10.1016/j.biotechadv.2023.108250. Online ahead of print.ABSTRACTEndolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. The use of genetic engineering techniques for the production and modification of endolysins offers the opportunity to customize their properties and activity against specific bacterial targets, paving the way for the development of personalized therapies for bacterial infections. Gram-negative bacteria possess an outer ...
Source: Biotechnology Advances - September 7, 2023 Category: Biotechnology Authors: Jose Vicente Carratal á Anna Ar ís Elena Garcia-Fruit ós Neus Ferrer-Miralles Source Type: research
Design strategies for positively charged endolysins: Insights into Artilysin development
Biotechnol Adv. 2023 Sep 5:108250. doi: 10.1016/j.biotechadv.2023.108250. Online ahead of print.ABSTRACTEndolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. The use of genetic engineering techniques for the production and modification of endolysins offers the opportunity to customize their properties and activity against specific bacterial targets, paving the way for the development of personalized therapies for bacterial infections. Gram-negative bacteria possess an outer ...
Source: Biotechnology Advances - September 7, 2023 Category: Biotechnology Authors: Jose Vicente Carratal á Anna Ar ís Elena Garcia-Fruit ós Neus Ferrer-Miralles Source Type: research
Design strategies for positively charged endolysins: Insights into Artilysin development
Biotechnol Adv. 2023 Sep 5:108250. doi: 10.1016/j.biotechadv.2023.108250. Online ahead of print.ABSTRACTEndolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. The use of genetic engineering techniques for the production and modification of endolysins offers the opportunity to customize their properties and activity against specific bacterial targets, paving the way for the development of personalized therapies for bacterial infections. Gram-negative bacteria possess an outer ...
Source: Biotechnology Advances - September 7, 2023 Category: Biotechnology Authors: Jose Vicente Carratal á Anna Ar ís Elena Garcia-Fruit ós Neus Ferrer-Miralles Source Type: research
Sulfide in engineered methanogenic systems - Friend or foe?
Biotechnol Adv. 2023 Sep 2:108249. doi: 10.1016/j.biotechadv.2023.108249. Online ahead of print.ABSTRACTSulfide ions are regarded to be toxic to microorganisms in engineered methanogenic systems (EMS), where organic substances are anaerobically converted to products such as methane, hydrogen, alcohols, and carboxylic acids. A vast body of research has addressed solutions to mitigate process disturbances associated with high sulfide levels, yet the established paradigm has drawn the attention away from the multifaceted sulfide interactions with minerals, organics, microbial interfaces and their implications for performance ...
Source: Biotechnology Advances - September 4, 2023 Category: Biotechnology Authors: Sepehr Shakeri Yekta Bo H Svensson Ulf Skyllberg Anna Schn ürer Source Type: research
CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals - current status, improvements, and perspectives
Biotechnol Adv. 2023 Sep 2:108248. doi: 10.1016/j.biotechadv.2023.108248. Online ahead of print.ABSTRACTCereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals (wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption, livestock feed, and breweries. Over the past half-century, modern varieties of cereal crops with increased yields have contributed to global food security. However, presently cultivated elite crop varieties were developed mainly for optimal environmental conditions. Thus, it has become evident that taking into account the ongoing climate changes...
Source: Biotechnology Advances - September 4, 2023 Category: Biotechnology Authors: Sunny Ahmar Goetz Hensel Damian Gruszka Source Type: research
Sulfide in engineered methanogenic systems - Friend or foe?
Biotechnol Adv. 2023 Sep 2:108249. doi: 10.1016/j.biotechadv.2023.108249. Online ahead of print.ABSTRACTSulfide ions are regarded to be toxic to microorganisms in engineered methanogenic systems (EMS), where organic substances are anaerobically converted to products such as methane, hydrogen, alcohols, and carboxylic acids. A vast body of research has addressed solutions to mitigate process disturbances associated with high sulfide levels, yet the established paradigm has drawn the attention away from the multifaceted sulfide interactions with minerals, organics, microbial interfaces and their implications for performance ...
Source: Biotechnology Advances - September 4, 2023 Category: Biotechnology Authors: Sepehr Shakeri Yekta Bo H Svensson Ulf Skyllberg Anna Schn ürer Source Type: research
CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals - current status, improvements, and perspectives
Biotechnol Adv. 2023 Sep 2:108248. doi: 10.1016/j.biotechadv.2023.108248. Online ahead of print.ABSTRACTCereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals (wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption, livestock feed, and breweries. Over the past half-century, modern varieties of cereal crops with increased yields have contributed to global food security. However, presently cultivated elite crop varieties were developed mainly for optimal environmental conditions. Thus, it has become evident that taking into account the ongoing climate changes...
Source: Biotechnology Advances - September 4, 2023 Category: Biotechnology Authors: Sunny Ahmar Goetz Hensel Damian Gruszka Source Type: research
Sulfide in engineered methanogenic systems - Friend or foe?
Biotechnol Adv. 2023 Sep 2:108249. doi: 10.1016/j.biotechadv.2023.108249. Online ahead of print.ABSTRACTSulfide ions are regarded to be toxic to microorganisms in engineered methanogenic systems (EMS), where organic substances are anaerobically converted to products such as methane, hydrogen, alcohols, and carboxylic acids. A vast body of research has addressed solutions to mitigate process disturbances associated with high sulfide levels, yet the established paradigm has drawn the attention away from the multifaceted sulfide interactions with minerals, organics, microbial interfaces and their implications for performance ...
Source: Biotechnology Advances - September 4, 2023 Category: Biotechnology Authors: Sepehr Shakeri Yekta Bo H Svensson Ulf Skyllberg Anna Schn ürer Source Type: research
