Systematic review on carrageenolytic enzymes: From metabolic pathways to applications in biotechnology
Biotechnol Adv. 2024 Apr 4:108351. doi: 10.1016/j.biotechadv.2024.108351. Online ahead of print.ABSTRACTCarrageenan, the major carbohydrate component of some red algae, is an important renewable bioresource with very large annual outputs. Different types of carrageenolytic enzymes in the carrageenan metabolic pathway are potentially valuable for the production of carrageenan oligosaccharides, biofuel, and other chemicals obtained from carrageenan. However, these enzymes are not well-developed for oligosaccharide or biofuel production. For further application, comprehensive knowledge of carrageenolytic enzymes is essential....
Source: Biotechnology Advances - April 6, 2024 Category: Biotechnology Authors: Chengcheng Jiang Yuqi Ma Wei Wang Jingjing Sun Jianhua Hao Xiangzhao Mao Source Type: research
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications
Biotechnol Adv. 2024 Apr 3;72:108352. doi: 10.1016/j.biotechadv.2024.108352. Online ahead of print.ABSTRACTNitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting environmental pressures, propelling them into the forefront of biodegradation and bioremediation endeavors. Neverthel...
Source: Biotechnology Advances - April 4, 2024 Category: Biotechnology Authors: Shi-Peng Zhou Ya-Ping Xue Yu-Guo Zheng Source Type: research
Maximizing the potential of nitrilase: Unveiling their diversity, catalytic proficiency, and versatile applications
Biotechnol Adv. 2024 Apr 2:108352. doi: 10.1016/j.biotechadv.2024.108352. Online ahead of print.ABSTRACTNitrilases represent a distinct class of enzymes that play a pivotal role in catalyzing the hydrolysis of nitrile compounds, leading to the formation of corresponding carboxylic acids. These enzymatic entities have garnered significant attention across a spectrum of industries, encompassing pharmaceuticals, agrochemicals, and fine chemicals. Moreover, their significance has been accentuated by mounting environmental pressures, propelling them into the forefront of biodegradation and bioremediation endeavors. Nevertheless...
Source: Biotechnology Advances - April 4, 2024 Category: Biotechnology Authors: Shi-Peng Zhou Ya-Ping Xue Yu-Guo Zheng Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Inteins: A Swiss army knife for synthetic biology
Biotechnol Adv. 2024 Mar 27:108349. doi: 10.1016/j.biotechadv.2024.108349. Online ahead of print.ABSTRACTInteins are proteins found in nature that execute protein splicing. Among them, split inteins stand out for their versatility and adaptability, presenting creative solutions for addressing intricate challenges in various biological applications. Their exquisite attributes, including compactness, reliability, orthogonality, low toxicity, and irreversibility, make them of interest to various fields including synthetic biology, biotechnology and biomedicine. In this review, we delve into the inherent challenges of using in...
Source: Biotechnology Advances - March 29, 2024 Category: Biotechnology Authors: Stanislav Anastassov Maurice Filo Mustafa Khammash Source Type: research
Expanding the horizon of transient CAR T therapeutics using virus-free technology
Biotechnol Adv. 2024 Mar 26;72:108350. doi: 10.1016/j.biotechadv.2024.108350. Online ahead of print.ABSTRACTThe extraordinary success that chimeric antigen receptor (CAR) T cell therapies have shown over the years on fighting hematological malignancies is evidenced by the six FDA-approved products present on the market. CAR T treatments have forever changed the way we understand cellular immunotherapies, as current research in the topic is expanding even outside the field of cancer with very promising results. Until now, virus-based strategies have been used for CAR T cell manufacturing. However, this methodology presents ...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Lucia Enriquez-Rodriguez Noha Attia Idoia Gallego Mohamed Mashal Iv án Maldonado Gustavo Puras Jos é Luis Pedraz Source Type: research
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy
Biotechnol Adv. 2024 Mar 26;72:108340. doi: 10.1016/j.biotechadv.2024.108340. Online ahead of print.ABSTRACTAs an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering strategies can further improve the efficiency of substrate assimilation and PHB synthesis. In the downstream link...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Jianfei Wang Jiaqi Huang Shijie Liu Source Type: research
Expanding the horizon of transient CAR T therapeutics using virus-free technology
Biotechnol Adv. 2024 Mar 25:108350. doi: 10.1016/j.biotechadv.2024.108350. Online ahead of print.ABSTRACTThe extraordinary success that chimeric antigen receptor (CAR) T cell therapies have shown over the years on fighting hematological malignancies is evidenced by the six FDA-approved products present on the market. CAR T treatments have forever changed the way we understand cellular immunotherapies, as current research in the topic is expanding even outside the field of cancer with very promising results. Until now, virus-based strategies have been used for CAR T cell manufacturing. However, this methodology presents rel...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Lucia Enriquez-Rodriguez Noha Attia Idoia Gallego Mohamed Mashal Iv án Maldonado Gustavo Puras Jos é Luis Pedraz Source Type: research
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy
Biotechnol Adv. 2024 Mar 26;72:108340. doi: 10.1016/j.biotechadv.2024.108340. Online ahead of print.ABSTRACTAs an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering strategies can further improve the efficiency of substrate assimilation and PHB synthesis. In the downstream link...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Jianfei Wang Jiaqi Huang Shijie Liu Source Type: research
Expanding the horizon of transient CAR T therapeutics using virus-free technology
Biotechnol Adv. 2024 Mar 25:108350. doi: 10.1016/j.biotechadv.2024.108350. Online ahead of print.ABSTRACTThe extraordinary success that chimeric antigen receptor (CAR) T cell therapies have shown over the years on fighting hematological malignancies is evidenced by the six FDA-approved products present on the market. CAR T treatments have forever changed the way we understand cellular immunotherapies, as current research in the topic is expanding even outside the field of cancer with very promising results. Until now, virus-based strategies have been used for CAR T cell manufacturing. However, this methodology presents rel...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Lucia Enriquez-Rodriguez Noha Attia Idoia Gallego Mohamed Mashal Iv án Maldonado Gustavo Puras Jos é Luis Pedraz Source Type: research
The production, recovery, and valorization of polyhydroxybutyrate (PHB) based on circular bioeconomy
Biotechnol Adv. 2024 Mar 25:108340. doi: 10.1016/j.biotechadv.2024.108340. Online ahead of print.ABSTRACTAs an energy-storage substance of microorganisms, polyhydroxybutyrate (PHB) is a promising alternative to petrochemical polymers. Under appropriate fermentation conditions, PHB-producing strains with metabolic diversity can efficiently synthesize PHB using various carbon sources. Carbon-rich wastes may serve as alternatives to pure sugar substrates to reduce the cost of PHB production. Genetic engineering strategies can further improve the efficiency of substrate assimilation and PHB synthesis. In the downstream link, P...
Source: Biotechnology Advances - March 27, 2024 Category: Biotechnology Authors: Jianfei Wang Jiaqi Huang Shijie Liu Source Type: research
Rational construction of synthetic consortia: Key considerations and model-based methods for guiding the development of a novel biosynthesis platform
Biotechnol Adv. 2024 Mar 24:108348. doi: 10.1016/j.biotechadv.2024.108348. Online ahead of print.ABSTRACTThe rapid development of synthetic biology has significantly improved the capabilities of mono-culture systems in converting different substrates into various value-added bio-chemicals through metabolic engineering. However, overexpression of biosynthetic pathways in recombinant strains can impose a heavy metabolic burden on the host, resulting in imbalanced energy distribution and negatively affecting both cell growth and biosynthesis capacity. Synthetic consortia, consisting of two or more microbial species or strains...
Source: Biotechnology Advances - March 26, 2024 Category: Biotechnology Authors: Yu Liu Boyuan Xue Hao Liu Shaojie Wang Haijia Su Source Type: research
