Antidiabetic, anti-hyperlipidemic and antioxidant activities of Bauhinia variegata flower extract
Publication date: Available online 17 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Abhishek K. Tripathi, Pushpraj S. Gupta, Sunil K. SinghAbstractTo evaluate antidiabetic, anti-hyperlipidemic and antioxidant activities of ethanolic extract of Bauhinia variegata flower. Ethanolic extract of B. variegata was administered orally to Streptozotocin (STZ) induced diabetic rats once daily for 21 days. Blood glucose levels were estimated at day 0, 7, 14 and 21 by glucometer (one touch) and lipid profile and histopathological examination of isolated organs (kidney, liver and pancreas) were also estimated ...
Source: Biocatalysis and Agricultural Biotechnology - April 19, 2019 Category: Biotechnology Source Type: research

Phospholipase A2-Mediated preparation of phosphatidylcholine containing ricinoleic acid and its anti-inflammatory effect on murine macrophage-like RAW264.7 cells
Publication date: Available online 17 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Yukihiro Yamamoto, Kazuki Harada, Suzuna Kasuga, Masashi HosokawaAbstractRicinoleic acid (RA) is a type of fatty acid found in castor oil and has been known to have anti-inflammatory effects. Phospholipids are useful functional compounds in food/medical fields due to their amphiphilic property and biocompatibility. The purpose of this study was to prepare phospholipid (PL) containing RA and to evaluate the anti-inflammatory activity of a prepared PL in vitro. Lyso-phosphatidylcholine (LPC) from egg yolk was subject...
Source: Biocatalysis and Agricultural Biotechnology - April 19, 2019 Category: Biotechnology Source Type: research

Efficacy of Andrographis paniculata against AmpC producing multi drug resistant E. coli
Publication date: Available online 18 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Saroj Kumar Sah, Ubaid Rasool, D. Mubarak Ali, S. HemalathaAbstractAndrographis paniculata (Acanthaceae) is a well-known medicinal plant and is conventionally used in herbal medicine for the treatment of several diseases such as cancer, metabolic disorders, skin infections, respiratory tract infections, etc. In this present study, ethyl acetate extract of A. paniculata was used to test its efficacy on controlling growth and biofilm formation in multi drug resistant pathogenic strains of Escherichia coli through mic...
Source: Biocatalysis and Agricultural Biotechnology - April 19, 2019 Category: Biotechnology Source Type: research

Nanoporous Zeolite-X as a new carrier for laccase immobilization and its application in dyes decolorization
Publication date: Available online 16 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Hala R. Wehaidy, Mohamed A. Abdel-Naby, Heba M. El-Hennawi, Hanan F. YoussefAbstractLaccase enzyme from Polyporus durus ATCC 26726 was immobilized on nanoporous Zeolite-X (ZX), with 83% immobilization yield. The immobilization process protected laccase against pH and temperature changes. The activation energy was lowered after immobilization. Compared to the free enzyme the immobilized enzyme displayed higher Km value and lower Vmax. The immobilized laccase exhibited higher half-life values (t1/2), higher decimal r...
Source: Biocatalysis and Agricultural Biotechnology - April 18, 2019 Category: Biotechnology Source Type: research

Panchagavya mediated copper nanoparticles synthesis, characterization and evaluating cytotoxicity in brine shrimp.
In this study, biosynthesis of Copper nanoparticles using Indian traditional medicine, Panchagavya is used which is eco-friendly, simple, cost effective and non-toxic. Copper sulphate (25 mM) solution was mixed with Panchagavya filtrate for the synthesis of Copper nanoparticles. The Nano metallic dispersion was characterized ultra violet spectrophotometer, Fourier transmission infrared ray spectroscopy, X-ray diffraction, Dynamic light scattering, Laser Raman, Scanning electron microscope, High resolution transmission electron microscope, X-ray fluorescent microscope. In addition, we studied the antioxidant and also cyto...
Source: Biocatalysis and Agricultural Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Probiotic bacteria promote the growth of associating host(red seaweed, Gracilaria edulis) also synthesize antibacterial protein
In this study, isolates of Gram positive Lysinibacillus xylanilyticus associated with red seaweed species were evaluated for antibacterial activity against plant pathogenic bacterium X. oryzae pv. oryzae which cause bacterial blight in rice using disc and well diffusion assays. The isolate HVT234 among the isolates of L. xylanilyticus associated with red seaweed species exhibit high antibacterial activity. The optimum level of extracellular antibacterial protein synthesize in HVT234 was recorded when cultured in 1.5% inoculum in seawater medium with lactose carbon and ammonium chloride nitrogen at pH 7.0 at 35 °C f...
Source: Biocatalysis and Agricultural Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Anticancer and potential bioactive studies of TiO2 nanoparticles synthesized using Glycyrrhiza glabra
Publication date: Available online 16 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Muthiah Bavanilatha, Lakshmanan Yoshitha, S. Nivedhitha, S. SahithyaAbstractThe world is fascinated by the emergence of nano products with the venture of nanotechnology with indomitable scientific advancements in almost all domains. The most interesting aspect of nanomaterials involves its synthesis protocol using green chemistry principles. In the event of eco-friendly approach, TiO2 nanoparticles were synthesized using Glycyrrhiza glabra root extracts from titanium oxysulfate precursor. These TiO2 nanoparticles w...
Source: Biocatalysis and Agricultural Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Chemical constituents and larvicidal efficacy of Naringi crenulata (Rutaceae) plant extracts and bioassay guided fractions against Culex quinquefasciatus mosquito (Diptera: Culicidae)
Publication date: Available online 16 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): T. Pratheeba, P. Vivekanandhan, A.K. Nur Faeza, D. NatarajanAbstractMosquitoes transmitted several vector borne diseases like malaria, dengue, lymphatic filariasis and now Zika virus. Synthetic chemicals cause much illness to humans, non-target organisms and natural ecosystem. In the present study, larvicidal efficacy of Naringi crenulata (Rutaceae) different leaf extracts and bioassay guided fractions against Culex quinquefasciatus, phytochemical screening and secondary metabolites were characterized by TLC, HPLC,...
Source: Biocatalysis and Agricultural Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Immobilization of Burkholderia cepacia lipase on crosslinked chitosan-based support for the synthesis of geranyl acetate
In this study, glutaraldehyde-crosslinked chitosan beads were prepared and characterized for the immobilization of Burkholderia cepacia lipase (BCL, 23,000 U/g). The crosslinking time and the concentration of glutaraldehyde were evaluated. The morphological modifications and the chemical interaction between the chitosan beads and the crosslinking agent were investigated by Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and ninhydrin assay. In general, both the concentration of glutaraldehyde (1.25 and 5.0% v/v) and crosslinking time (6 and 12 h) showed a minor influence on the intens...
Source: Biocatalysis and Agricultural Biotechnology - April 16, 2019 Category: Biotechnology Source Type: research

Spectral characterization of bioactive compounds from microalgae: N. Oculata and C. Vulgaris
Publication date: Available online 14 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): P. Bhuvana, P. Sangeetha, V. Anuradha, M. Syed AliAbstractMicroalgae are unicellular eukaryotic organisms that thrive in a wide variety of habitats such as fresh, salt water and marine environment. Microalgae are considered to be a good source of fatty acids, pigments and various other bioactive metabolites. Hence, the current study attempts to identify bioactive metabolites from two microalgae species: Nannochloropsis Oculata and Chlorella Vulgaris. This diverse group of autotrophic organisms can rapidly produce l...
Source: Biocatalysis and Agricultural Biotechnology - April 14, 2019 Category: Biotechnology Source Type: research

Corrigendum to “Diarylheptanoids as nutraceutical: A review” [Biocatal. Agric. Biotechnol. 19 (2019) 101109]
Publication date: Available online 6 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): G. Ganapathy, R. Preethi, J.A. Moses, C. Anandharamakrishnan (Source: Biocatalysis and Agricultural Biotechnology)
Source: Biocatalysis and Agricultural Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Cytotoxicity study and antioxidant activity of crude extracts and SPE fractions from Carica papaya leaves
Publication date: Available online 11 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Fitrien Husin, Harisun Ya'akob, Siti Norazlina Abd Rashid, Saleha Shahar, Husnul Hanani SoibAbstractMillions of people in various traditional systems resorted to the use of medicinal plants for their ailments and recently, substances with antioxidant properties have been given unprecedented attention as possible therapeutic and preventive agents. The present study focused on cytotoxicity and antioxidant assessment of crude and fractions of C. papaya leaves as an important medicinal plant. Cytotoxicity was measured ...
Source: Biocatalysis and Agricultural Biotechnology - April 13, 2019 Category: Biotechnology Source Type: research

Probing the biomolecular targets of azo colorant carcinogens towards purified wetland peroxidase-computational cum in vitro validation
Publication date: Available online 10 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Sai Preethi, M. RameshpathyAbstractAzo dyes are attributed to be noxious to aquatic organisms impeding the endocrinal and metabolic phenomenon. Moreover, they are dwelled to exhibit carcinogenesis, teratogenesis and mutagenesis on humans and animals. Hence, this study explicitly targets on eradication of azo dye toxicity by microbial peroxidase as a robust tool. A novel peroxidase producing wetland isolate, Streptomyces coelicolor strain SPR7 was bioprospected. Computational prediction of chemical properties of syn...
Source: Biocatalysis and Agricultural Biotechnology - April 12, 2019 Category: Biotechnology Source Type: research

Phosphate solubilizing and PGR activities of ericaceous shrubs microorganisms isolated from Mediterranean forest soil
Publication date: Available online 11 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Ahlam Hamim, Amal Boukeskasse, Yedir Ouhdouch, Abderhmane Farrouki, Said Barrijal, Lucie Miché, Rachid Mrabet, Robin Duponnois, Mohamed HafidiAbstractMany soil microorganisms are able to transform insoluble forms of phosphorus into an accessible soluble form, thus contributing to plant nutrition through exhibiting other beneficial traits such as production of organic acids, siderophore indole acetic acid (IAA) and production of hydrogen cyanide (HCN). Achieving this purpose, ericoid fungi and actinobacteria ...
Source: Biocatalysis and Agricultural Biotechnology - April 12, 2019 Category: Biotechnology Source Type: research

Cerium oxide nanocluster based nanobiosensor for ROS detection
Publication date: Available online 9 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): V. Thendral, T. Dharshni, M. Ramalakshmi, Agnishwar Girigoswami, Koyeli GirigoswamiAbstract:The present study demonstrates the synthesis of cerium oxide nanoclusters (CeO2-NC) and its application towards detection of reactive oxygen species (ROS). ROS are generated due to exogenous exposure to oxidants, car smoke, pollutants etc. as well as endogenously as a byproduct of metabolism that can cause cellular damage. Although, protective antioxidant molecules and antioxidant enzymes are present to combat these oxidative...
Source: Biocatalysis and Agricultural Biotechnology - April 10, 2019 Category: Biotechnology Source Type: research

Production optimisation and characterisation of extracellular protease secreted by newly isolated Bacillus subtilis AU-2 strain obtained from Tribolium castaneum gut
Publication date: Available online 8 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Aniketa R. Patel, Narendra U. Mokashe, Diptaraj S. Chaudhari, Ajay G. Jadhav, Ulhas K. PatilAbstractA novel protease secreting bacterial strain was isolated from the gut of Tribolium castaneum residing in stored Glycine max (soybean) seeds. The strain was identified as Bacillus subtilis AU-2. A response surface approach using Plakett-Burman Design (PBD) was used for the screening and a Central Composite Design (CCD) was used for the optimization of production parameters. An elevated protease production (580 U/mL) wa...
Source: Biocatalysis and Agricultural Biotechnology - April 10, 2019 Category: Biotechnology Source Type: research

Augmenting a competent in vitro organogenesis etiquette from leaf base of country mallow, Abutilon indicum L. sweet: An ethno-botanically valuable medicinal plant
Publication date: Available online 5 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Subramanian Radhesh Krishnan, Subramani Pandian, Ramachandradoss Banupriya, Pandiyan Muthuramalingam, Syed Jeritha Banu, Manikandan Arumugam, Manikandan RameshAbstractAn easy, efficient and highly reproducible regeneration system was established through organogenesis from leaf base mediated callus of an ethnobotanical hairy shrub Abutilon indicum L. sweet, which is documented to possess pharmaceutically important phytochemicals. Among various explants, leaf bases produced significant callus induction (89%) with 140...
Source: Biocatalysis and Agricultural Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Corrigendum to “Diarylheptanoids as nutraceutical: A review” [Biocatalysis and Agricultural Biotechnology Vol. 19 (2019) 101109]
Publication date: Available online 6 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): G. Ganapathy, R. Preethi, J.A. Moses, C. Anandharamakrishnan (Source: Biocatalysis and Agricultural Biotechnology)
Source: Biocatalysis and Agricultural Biotechnology - April 7, 2019 Category: Biotechnology Source Type: research

Response surface optimization of a glycolipid biosurfactant produced by a sponge associated marine bacterium Planococcus sp. MMD26
Publication date: March 2019Source: Biocatalysis and Agricultural Biotechnology, Volume 18Author(s): T. Hema, G. Seghal Kiran, Arya Sajayyan, Amrudha Ravendran, G. Gowtham Raj, Joseph SelvinAbstractMarine sponge associated bacterium Planococcus sp. MMD26 was isolated and selected for biosurfactant production. Criteria for screening of biosurfactant production by MMD26 were hemolytic activity, oil displacement test and drop collapsing test. Based on the response surface methodology (RSM)-based optimization experiments the biosurfactant production was enhanced to one fold over the wild strain. The biosurfactant produced by t...
Source: Biocatalysis and Agricultural Biotechnology - April 5, 2019 Category: Biotechnology Source Type: research

Purification of a novel monophenolase inhibitory peptides prepared from Vicia faba pods protein via enzymatic hydrolysis
Publication date: Available online 4 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Sanaa T. El-Sayed, Rehab A. Al- Azzouny, Ola S. AliAbstractThe aim of the present study was to prepare novel monophenolase inhibitory peptides from Vicia faba (broad bean) pods. Isolated protein was hydrolysed by immobilized protease at pH 10. For maximum production of hydrolysate (peptides), an optimized hydrolysis process, including incubation temperature and protein concentration, was established. Broad bean peptides had higher tyrosinase inhibitor potency than that of the parent protein. They were fractionated b...
Source: Biocatalysis and Agricultural Biotechnology - April 5, 2019 Category: Biotechnology Source Type: research

Purification and partial characterization of seed lectins from Vicias belonging to subgenus Vicilla section cracca
Publication date: Available online 4 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Cristina Megias, Isabel Cortés-Giraldo, Julio Giron-Calle, Manuel Alaiz, Javier VioqueAbstractLectins from the seeds of ten Vicias belonging to section Cracca (V. benghalensis, V. dasycarpa, V. monantha, V. villosa, V. cracca, V. vicioides, V. pseudocracca, V. disperma, V. tenuifolia and V. monardii) have been purified by ultrafiltration and gel filtration chromatography, and characterized. All lectins belonged to the single-chain legume lectin group, having four subunits with molecular weights around 35&ndas...
Source: Biocatalysis and Agricultural Biotechnology - April 5, 2019 Category: Biotechnology Source Type: research

Intestinal adsorption of glucose, cholesterol and bile salt by simultaneous incorporation of edible microbiosorbent and intestinal bacteria
Publication date: Available online 4 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Alireza Chackoshian Khorasani, Seyed Abbas ShojaosadatiAbstractNatural edible adsorbents can play an important role in human healthcare via lowering the harmful materials from gut and regulating the microflora composition. In this work, several microalgae and plant sources, including Chlorella vulgaris (Ch), zucchini (Zu), ginger (Gi), rice bran (RB) and Zahedi date (ZD), were studied on the production of edible and bacterial biosorbents for the adsorption of glucose, cholesterol and bile salt in vitro. By screening...
Source: Biocatalysis and Agricultural Biotechnology - April 5, 2019 Category: Biotechnology Source Type: research

Characterization of mutamba (Guazuma ulmifolia LAM.) fruit flour and development of bread
Publication date: Available online 3 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Renato Queiroz Assis, Karina Luzia Andrade, Lílian Emanoela Gomes Batista, Alessandro de Oliveira Rios, Disney Ribeiro Dias, Eliane Augusto Ndiaye, Éllen Cristina de SouzaAbstractThis work aims to characterise chemical and phytochemical mutamba (Guazuma ulmifolia LAM.) flour and evaluate its addition in whole wheat bread. The phytochemical analysis revealed the presence of phenols, tannins, saponins, steroids, resins, quaternary bases, quinones, flavonoids and triterpenoids. For the test of Artemia sal...
Source: Biocatalysis and Agricultural Biotechnology - April 5, 2019 Category: Biotechnology Source Type: research

Pellet diameter and morphology of European Ganoderma pfeifferi in a repeated-batch fermentation for exopolysaccharide production
Publication date: Available online 3 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Sugenendran Supramani, Nurulhuda Jailani, Kivaandra Ramarao, Nurul Amalina Mohd Zain, Anita Klaus, Rahayu Ahmad, Wan Abd Al Qadr Imad Wan-MohtarAbstractThe pellet diameter and morphology of an underutilized European Ganoderma pfeifferi (EGP) mycelium was studied in a repeated-batch fermentation (RBF) for exopolysaccharide (EPS) production. In preliminary fermentation, growth screening was performed for initial pH, glucose concentration, and agitation speed. At 30 °C, the favourable conditions for EPS productio...
Source: Biocatalysis and Agricultural Biotechnology - April 4, 2019 Category: Biotechnology Source Type: research

In vitro antileishmanial and antioxidant potential, cytotoxicity evaluation and phytochemical analysis of extracts from selected medicinally important plants
Publication date: Available online 1 April 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Shaila Mehwish, Arshad Islam, Ikram Ullah, Abdul Wakeel, Muhammad Qasim, Mubarak Ali Khan, Ayaz Ahmad, Nazif UllahAbstractThe antileishmanial drugs presently available have stern limitations regarding efficiency, safety and cost that make it crucial to hunt for novel therapeutic approaches from natural products. In current study crude methanolic extracts (CMEs) of four medicinally important plants were evaluated for their phytochemical constituents, antioxidant potentials, cytotoxicity and antileishmanial activities...
Source: Biocatalysis and Agricultural Biotechnology - April 2, 2019 Category: Biotechnology Source Type: research

Effects of different extracting conditions on anti-tyrosinase and antioxidant activities of Schizophyllum commune fruit bodies
The objective of this preliminary study was to evaluate the effect of extraction conditions on the cosmeceutical properties of S. commune extract. Fruit bodies were extracted using two different temperatures; 4 °C and 30 °C at three different extraction times; 1, 12 and 24 h, using water as extracting solvent. Anti-pigmentation activity measured by a tyrosinase inhibition assay, as well as antioxidant activities, were evaluated. Biological components such as total phenolic, polysaccharide, and glucan content were also assessed. The results revealed that a 1 h extraction time at 4 °C or 30 °C pro...
Source: Biocatalysis and Agricultural Biotechnology - April 2, 2019 Category: Biotechnology Source Type: research

Antihypercholesterolemic, antioxidative and anti-inflammatory potential of an extract of the plant Tabernaemontana divaricata in experimental rats fed an atherogenic diet
Publication date: Available online 30 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Muniyandi Anbukkarasi, Mahalingam Sundararajan, Karuppasamy Venkadeswaran, Vasanthakumar Vasantha Ruban, Thiraviyam Anand, Pitchairaj GeraldineAbstractThe present study highlights the putative anti-atherogenic, antioxidative and anti-inflammatory potential of Tabernaemontana divaricata extract (ethanolic) in a Wistar rat model of experimental hypercholesterolemia. The rats were divided into Group I (control), Group II (atherogenic diet-fed, saline-treated) and Group III (atherogenic diet-fed, T. divaricata extract-...
Source: Biocatalysis and Agricultural Biotechnology - March 31, 2019 Category: Biotechnology Source Type: research

Cloning, expression, purification and characterization of a thermo- and surfactant-stable protease from Thermomonospora curvata
Publication date: Available online 26 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Duangjai Sittipol, Pijitra Saelao, Tassanee Lohnoo, Tassanee Lerksuthirat, Yothin Kumsang, Wanta Yingyong, Pongsak Khunrae, Triwit Rattanarojpong, Nujarin JongrujaAbstractProtease is widely used in various industrial applications to hydrolyze the peptide bonds in protein molecules. The appropriate biochemical characteristics of the proteases for the industry, especially thermostability of the enzyme, are the key requirement for the potential applications. Therefore, we identified, expressed, purified and characteri...
Source: Biocatalysis and Agricultural Biotechnology - March 27, 2019 Category: Biotechnology Source Type: research

Bioremoval of toxic substances in synthetic wastewater using Trichoderma pubescens (NPK2), isolated from mangrove soil
Publication date: Available online 26 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): R. Narendran, K. Kathiresan, R.S. Sathishkumar, K. Kayalvizhi, A. Sundaramanickam (Source: Biocatalysis and Agricultural Biotechnology)
Source: Biocatalysis and Agricultural Biotechnology - March 27, 2019 Category: Biotechnology Source Type: research

Diarylheptanoids as nutraceutical: A review
Publication date: Available online 26 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Ganapathy G, Preethi R, J.A. Moses, C. AnandharamakrishnanAbstractPhenolic compounds are naturally occurring compounds present ubiquitously in plants. They have potential health benefits and substantiate evidence for their nutraceutical applications. Diarylheptanoids are part of the broad class of plant phenolics with structurally divergent compounds. They have been used in traditional medicines and homemade remedies to treat various ailments, as organoleptic additives in foods, and also for aesthetic purposes. Wit...
Source: Biocatalysis and Agricultural Biotechnology - March 27, 2019 Category: Biotechnology Source Type: research

Glycosylation of caffeic acid and structural analogues catalyzed by novel glucansucrases from Leuconostoc and Weissella species
Publication date: Available online 26 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Johannes Nolte, Alexander Kempa, Arne Schlockermann, Matthias Hochgürtel, Ulrich SchörkenAbstractTwelve Leuconostoc and seven Weissella strains with extracellular glucansucrase activity were obtained from an analysis of 41 lactic acid bacteria. Culture supernatants of all glucansucrase positive strains catalyzed the glycosylation of caffeic acid with sucrose as donor substrate. Eighteen enzymes produced one major peak, which was identified as caffeic acid-4′O-α-D-monoglucoside by LC-MS and NMR...
Source: Biocatalysis and Agricultural Biotechnology - March 27, 2019 Category: Biotechnology Source Type: research

Production of biofuel from sugarcane molasses by diazotrophic Bacillus and recycle of spent bacterial biomass as biofertilizer inoculants for oil crops
This study was designed to identify the most potent N2-fixing and biofuel producing Bacillus species. Four isolates were selected as the most efficient N2-fixing organisms which confirmed by nifH gene expression. These isolates were identified genetically by 16S rRNA as Bacillus thuringensis, Bacillus subtilis, Bacillus pumilus and Bacillus licheniformis. The highest biohydrogen production was 2450 and 2300 ml/L from 6% sugarcane molasses by B. thuringensis and B. subtilis respectively. Nitrogenase activity of B. thuringensis and B. subtilis were 1.4 and 1.3 μmol C2H4 min−1 mg protein −1 at 6% molasses. ...
Source: Biocatalysis and Agricultural Biotechnology - March 27, 2019 Category: Biotechnology Source Type: research

Evaluation of heavy metals tolerant bacterial strains as antioxidant agents and plant growth promoters
This study aimed to evaluate three heavy metal tolerant bacterial strains (HMTB) namely Alcaligenes faecalis MG257493.1, Bacillus cereus MG257494.1 and Alcaligenes faecalis MG966440.1 as antioxidant agents and plant growth promotors under laboratory conditions. Their abilities to inhibit 2,2-DiPhenyl-2-Picrylhydrazyl hydrate (DPPH) as non-enzymatic antioxidant activity and produce plant growth promoters (indole acetic acid (IAA), gibberellins (GA3), salicylic acid (SA), proline (Pro.), sidrophores, exopolysaccharide (EPS) and biosurfactant) were estimated in presence of four heavy metals Zinc (Zn2+) cadmium (Cd2+), copper ...
Source: Biocatalysis and Agricultural Biotechnology - March 25, 2019 Category: Biotechnology Source Type: research

Screening and characterization of microorganisms catalyzing (S)-selective oxidation of α,α-disubstituted propanediols
In this study, we attempted to achieve efficient synthesis of (S)-form 2-(hydroxymethyl)-2-methylbutanoic acid (HMMBA) from 2-ethyl-2-methyl-1,3-propanediol (EMPD) using an enzymatic approach. Agromyces sp. E2 was found to produce HMMBA from soil samples through enrichment culture using EMPD as a carbon source. The microbial asymmetric oxidation was characterized, and the highest activity was observed following addition of 0.2% (w/v) 2,2-diethyl-1,3-propanediol in the culture as an inducer. After 72 h of whole-cell incubation with 40 mM EMPD under optimized conditions, strain E2 completely converted EMPD to HMMBA with ...
Source: Biocatalysis and Agricultural Biotechnology - March 25, 2019 Category: Biotechnology Source Type: research

In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel. (Lamiaceae) – A medicinally important source of phenolic compounds
Publication date: Available online 24 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Shahla Ghaderi, Samad Nejad Ebrahimi, Hamid Ahadi, Sara Eslambolchi Moghadam, Mohammad Hossein MirjaliliAbstractPerovskia abrotanoides Karel. (Lamiaceae) is an aromatic herb that is mainly grown in the mountains of Northeastern Iran across Northern Pakistan and Northwestern India. Here, a suitable system for regenerating P. abrotanoides via direct organogenesis was performed for the first time. In vitro-grown shoot tips were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (1.0...
Source: Biocatalysis and Agricultural Biotechnology - March 25, 2019 Category: Biotechnology Source Type: research

Effect of extraction temperatures on tannin content and antioxidant activity of Quercus infectoria (Manjakani)
This study was specifically aimed to investigate the effects of extraction temperatures on tannin and antioxidant activity; the interaction between these two responses towards the processing parameter chosen. The main bioactive compound; tannin was extracted from Quercus infectoria (Manjakani) galls using aqueous decoction method. Few different extraction temperatures were selected prior for the extraction of tannin from Q. infectoria galls optimally. The selected temperatures were at 50, 75 and 100 °C and the effects of these temperatures on tannin content and antioxidant activity were examined thoroughly. High Perf...
Source: Biocatalysis and Agricultural Biotechnology - March 24, 2019 Category: Biotechnology Source Type: research

Isolation, optimization, and purification of extracellular levansucrase from nonpathogenic Klebsiella strain L1 isolated from waste sugarcane bagasse
Publication date: Available online 23 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Maitri Desai, Kartik PatelAbstractA newly isolated acidophilic bacterial strain from waste sugarcane bagasse was identified as Klebsiella species by biochemical and 16S rRNA sequencing methods. Klebsiella sp. strain L1 was selected for its ability to produce extracellular levansucrase. The optimization of carbon source, nitrogen source, sucrose concentration, NaCl concentration, initial pH and temperature of the growth medium in flask fermentation medium. In the investigation, sucrose acts as superior carbon source...
Source: Biocatalysis and Agricultural Biotechnology - March 24, 2019 Category: Biotechnology Source Type: research

Fungal strain impacts the shape, bioactivity and multifunctional properties of green synthesized zinc oxide nanoparticle
Publication date: Available online 24 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Asem A. Mohmed, Amr Fouda, Mohamed Ali Abdel-Rahman, Saad El-Din Hassan, Mamdouh S. El-Gamal, Salem S. Salem, Tharwat I. ShaheenAbstractThis work aimed to investigate the influence of fungal strains onto shape, functional properties, and potential applications of biosynthesized nanoparticles (NPs). The aqueous extract of two newly isolated fungal strains, Fusarium keratoplasticum strain (A1-3) and Aspergillus niger strain (G3-1), were used for synthesis of ZnO-NPs. Nanoparticles formation was confirmed by visual ob...
Source: Biocatalysis and Agricultural Biotechnology - March 24, 2019 Category: Biotechnology Source Type: research

In vitro and in vivo antitumor activity of deoxyelephantopin from a potential medicinal plant Elephantopus scaber against Ehrlich ascites carcinoma
Publication date: Available online 22 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Farha Arakkaveettil Kabeer, Dhanya Sethumadhavan Rajalekshmi, Mangalam Sivasankaran Nair, Remani PrathapanAbstractElephantopus scaber Linn. Is a perennial medicinal herb widely used in Asian traditional medicine for the treatment of different kinds of neoplasm. The aim of the present study was to appraise the in vitro and in vivo antitumor activity of deoxyelephantopin (DOE) - the active principle isolated from E. scaber against murine Ehrlich's ascites carcinoma (EAC). Intraperitoneal administration of DOE (25 m...
Source: Biocatalysis and Agricultural Biotechnology - March 23, 2019 Category: Biotechnology Source Type: research

The effect of temperature variation on the growth of Leptolyngbya (cyanobacteria) HS-16 and HS-36 to biomass weight in BG-11 medium
This study is expected to provide information on the temperature of growth ability of Leptolyngbya HS-16 and Leptolyngbya HS-36, so that storage conditions of culture space in subsequent research can be arranged to prevent these strains from dying or not growing well. In the utilization of cyanobacteria, a clear physiological characterization of the cyanobacteria is required. Temperature is one of the major factors affecting the growth of cyanobacteria. The growth differences between cyanobacteria strains Leptolyngbya HS-16 and Leptolyngbya HS-36 which were incubated in 20 °C, 35 °C, and 50 °C had been st...
Source: Biocatalysis and Agricultural Biotechnology - March 23, 2019 Category: Biotechnology Source Type: research

Use of low-cost substrates for cost-effective production of extracellular and cell-bound lipases by a newly isolated yeast Dipodascus capitatus A4C
Publication date: Available online 21 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Khurshid Ahmed Baloch, Apichat Upaichit, Benjamas CheirsilpAbstractAs the enzyme production and its application is a costly process mainly because of the inducible production, this study aimed to screen the basic components of media for the production of cost-effective lipases. A newly isolated yeast strain, namely Dipodascus capitatus A4C is a specific yeast that could produce lipases in both extracellular and cell-bound forms. Various low-cost substrates were applied as single or mixed carbon sources for D. capit...
Source: Biocatalysis and Agricultural Biotechnology - March 22, 2019 Category: Biotechnology Source Type: research

Properties of bromelain extract from different parts of the pineapple variety Morris
Publication date: Available online 19 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Effaliza Misran, Ani Idris, Siti Hajar Mat Sarip, Harisun Ya'akobAbstractThe purpose of this study is to characterize the bromelain extract from pineapples which belong to the Morris variety. The bromelain was extracted from two different batches of Morris pineapples; one with crown and the other without crown. The weight of each part such as flesh, peel, core, stem and crown were 45–52%, 28–29%, 13–14%, 5–6% and 7% (w/w), respectively. The physiochemical properties of each part of the pinea...
Source: Biocatalysis and Agricultural Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research

Maltose syrup residue as the substrate for Monascus pigments production
The objective of this study was to determine the effect of pH on the growth rate and production of pigments by Monascus ruber from maltose syrup residue as a substrate. The cultivations were carried out in solid and submerged media under different pH conditions. The highest radial growth rate occurred when the Monascus ruber was cultivated at pH 6.5 and the production of yellow pigments occurred at low pH values (2.0 and 2.5), whereas the production of orange pigments occurred at pH 3.0 and 3.5 and that of red pigments when the fungus was cultivated above pH 4.0. The results showed that the pH value had a significant influ...
Source: Biocatalysis and Agricultural Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research

Valorization of fruit by-products of Bromelia antiacantha Bertol.: Protease obtaining and its potential as additive for laundry detergents
Publication date: Available online 20 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Grisel Bersi, Diego Vallés, Fabricio Penna, Ana María Cantera, Sonia BarberisAbstractThe aim of this work is to isolate and study the performance of an alkaline protease from fruit by-products of Bromelia antiachanta Bertol. for its potential application as an additive of laundry detergent formulations. Its behavior is compared with Pura Felt 4000 (Genencor Argentina). Antiacanthain, a cysteine protease from fruit by-products of B. antiachanta, showed a remarkable stability (50 ± 0.82% to 1...
Source: Biocatalysis and Agricultural Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research

Prospects of using entomopathogenic fungus in development of a biopesticide product with nematicidal activity
The objective of this work was to evaluate the prospects of using an entomopathogenic fungus strain of Beauveria bassiana to develop a biological product, in native form and immobilized on a carrier, which would possess, among other characteristics, nematicide activity. In culture suspension based on a mixture of milk whey and brewing spent grain liquor, the product showed catalase and cellulolytic activity, as well as high yield of spore-bearing biomass. Insecticidal activity of the fungus has been determined relative to a test-organism - Drosophila melanogaster. Virulence was at 50% with the infective dose of 102 CFU. ...
Source: Biocatalysis and Agricultural Biotechnology - March 21, 2019 Category: Biotechnology Source Type: research

Nitrate (NO3−) and phosphate (PO43−) removal from aqueous solutions by microalgae Dunaliella salina
This study was conducted to investigate the mechanism involved in the removal of nitrate (NO3−) and phosphate (PO43−) by microalgae, Dunaliella salina from aqueous solutions through a series of batch experiments and FT-IR analyses. The effect of various operation parameters including pH of the solution, microalgae dosage, initial NO3− and PO43− concentrations and interference of other cations and anions on the uptake capacity and removal efficiency of D. salina was evaluated. The NO3− and PO43− concentrations were measured with spectrophotometric methods. The results showed that the maxi...
Source: Biocatalysis and Agricultural Biotechnology - March 19, 2019 Category: Biotechnology Source Type: research

The protective role of Piriformospora indica colonization in Centella asiatica (L.) in vitro under phosphate stress
Publication date: Available online 18 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): S. Jisha, K.N. Anith, K.K. SabuAbstractThe core of present study was to determine potential role of Piriformospora indica, mutualistic Basidiomycete endosymbiont on co-cultivation with Centella asiatica (L.) Urb., a neutraceutical plant. The analysis of major secondary metabolite asiaticoside and differential expression of key genes involved in pathway under varying phosphate (P) concentrations maintained in vitro were carried out. Maximum asiaticoside content was documented in plants maintained in MS medium contai...
Source: Biocatalysis and Agricultural Biotechnology - March 18, 2019 Category: Biotechnology Source Type: research

Quinoline derivatives producing Pseudomonas aeruginosa H6 as an efficient bioherbicide for weed management
Publication date: Available online 16 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Smisha Lawrance, Sherin Varghese, Edna Mary Varghese, Aju K. Asok, Jisha M. SAbstractRegular application of weedicides has led to multiple toxic effects on human health, environment, and food safety which also facilitated an emergence of weed resistance. Development of bioherbicides using microorganisms has attained considerable attention nowadays for weed management in agricultural fields. In the present study, a rhizospheric bacteria, H6, showing a weedicide efficacy was discovered from the rhizosphere of Momordi...
Source: Biocatalysis and Agricultural Biotechnology - March 17, 2019 Category: Biotechnology Source Type: research

Isolation and identification of a phosphate-solubilizing Paenibacillus polymyxa strain GOL 0202 from durum wheat (Triticum durum Desf.) rhizosphere and its effect on some seedlings morphophysiological parameters
In this study, we have identified GOL 0202 strain as Paenibacillus polymyxa, isolated from durum wheat (Triticum durum Desf.) rhizosphere collected from arid region soil of Southern. This genomic identification was performed by 16S rRNA gene sequencing. It was shown that this strain possesses an interesting phosphate solubilization activity on NBRIP (National Botanical Research Institute Phosphorus) medium containing tricalcium phosphate (Ca3 (PO4)2). This activity, was recorded over five days, it was negatively correlated with pH medium whose decline was due probably to the release of organic acids. In addition to other a...
Source: Biocatalysis and Agricultural Biotechnology - March 16, 2019 Category: Biotechnology Source Type: research

Covalent immobilization of soybean seed hull urease on chitosan mini-spheres and the impact on their properties
Publication date: Available online 14 March 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Lautaro Fidel Bracco, Gustavo Javier Levin, Nicolás Urtasun, Agustín Andrés Navarro del Cañizo, Federico Javier Wolman, María Victoria Miranda, Osvaldo CasconeAbstractUrease (EC 3.5.1.5) is found in bacteria, fungi, algae, plants and some invertebrates, and in soils. Soil ureases are partly extracellular being liberated during microbial and plant root metabolism and death. In plants, urease is present in most of Leguminosae seeds, accounting for 0.2–0.3% of the extractable s...
Source: Biocatalysis and Agricultural Biotechnology - March 15, 2019 Category: Biotechnology Source Type: research