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Total 393 results found since Jan 2013.

Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
Source: Springer protocols feed by Biotechnology - February 25, 2014 Category: Biotechnology Source Type: news

Development of Two-Component Nanorod Complex for Dual-Fluorescence Imaging and siRNA delivery.
This study demonstrates the feasibility of using two-component nanorods as a potential theranostic in breast cancer treatment with capabilities in dual imaging and targeted gene delivery. PMID: 24986677 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - July 2, 2014 Category: Biotechnology Authors: Choi JH, Oh BK Tags: J Microbiol Biotechnol Source Type: research

Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer–siRNA Nanoparticles Via the Sticky Bridge
Structured functional RNA entities, including aptamers and siRNAs, have amazing versatility in structure and function. These molecules can serve as powerful, attractive building blocks for the bottom-up assembly of complex nanostructures. Here, we describe novel cell-type specific and internalizing B-cell activating factor receptor (BAFF-R) aptamer–siRNA delivery systems for B-cell lymphoma therapy, in which both the aptamer and the Dicer substrate siRNA (DsiRNA) portions are conjugated through a “sticky bridge.” The BAFF-R is overexpressed on the surface of B-cell malignancies, allowing binding and inter...
Source: Springer protocols feed by Biotechnology - April 25, 2015 Category: Biotechnology Source Type: news

Preparation and evaluation of a non-viral gene vector for SiRNA: Multifunctional envelope-type nano device.
Authors: Zhang Y, Wei H, Xu L, Yan G, Ma C, Yu M, Wei C, Sun Y Abstract We prepared and evaluated a multifunctional envelope-type nano device (MEND) as a liver-targeting and long-circulation carrier for SiRNA. The polymer GA-PEG-Pp-DOPE was synthesized by modifying polyethylene glycol (PEG) with glycyrrhetinic acid (GA), peptide (Pp), and dioleoyl phosphoethanolamine (DOPE). The Pp is a substrate of matrix metalloproteinase 2. MEND was prepared with GA-PEG-Pp-DOPE and cationic phospholipids by the filming-rehydration method, and the orthogonal test was applied to optimize the prescription. The results of the biolog...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 12, 2015 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Identifying HIPK1 as target of miR-22-3p enhancing recombinant protein production from HEK 293 cell by using microarray and HTP siRNA screen.
Abstract Protein expression from human embryonic kidney cells (HEK 293) is an important tool for structural and clinical studies. It was previously shown that microRNAs (small, noncoding RNAs) are effective means for improved protein expression from these cells, and by conducting a high-throughput screening of the human microRNA library, several microRNAs were identified as potential candidates for improving expression. From these, miR-22-3p was chosen for further study since it increased the expression of luciferase, two membrane proteins and a secreted fusion protein with minimal effect on the cells' growth and ...
Source: Biotechnology Journal - October 7, 2017 Category: Biotechnology Authors: Inwood S, Buehler E, Betenbaugh M, Lal M, Shiloach J Tags: Biotechnol J Source Type: research

Cancer cell membrane cloaking nanoparticles for targeted co-delivery of doxorubicin and PD-L1 siRNA.
Authors: Chen M, Chen M, He J Abstract Nanoparticles coated with cell membranes have been garnering growing attention due to their homologous binding capability of membrane molecules and consequent self-recognition by their source cells. In the present study, we report on the construction of doxorubicin and PD-L1 siRNA-loaded PLGA nanoparticles and their biological functionalization by cancer cell-derived membrane cloaking. The resulting cancer cell membrane-coated nanoparticles (CCMNPs) presented a core-shell nanostructure with highly specific self-recognition affinity to the homotypic cells, which can be attribut...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 28, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Intranasal Delivery of Chitosan–siRNA Nanoparticle Formulation to the Brain
Neurodegeneration is characterized by a progressive loss of neuron structure and function. Most neurodegenerative diseases progress slowly over the time. There is currently no cure available for any neurodegenerative disease, and the existing therapeutic interventions only alleviate the symptoms of the disease. The advances in the drug discovery research have come to a halt with a lack of effective means to deliver drugs at the targeted site. In addition, the route of delivering the drugs is equally important as most invasive techniques lead to postoperative complications. This chapter focuses on a non-invasive, intranasal...
Source: Springer protocols feed by Biotechnology - February 25, 2014 Category: Biotechnology Source Type: news

siRNA mediated gene silencing in Fusarium sp. HKF15 for overproduction of bikaverin.
Abstract Fusarium sp. HKF15 is an isolate from effluent treatment plant which produces bikaverin. Bikaverin is a polyketide having antitumor and antibiotic potential. Acetyl coenzyme A is a common precursor for bikaverin as well as carotenoids and gibberellins. A polyketide synthase gene bik1 is responsible for bikaverin production whereas, hydroxymethyl glutaryl coenzyme A reductase (hmgR) and farnesyl pyrophosphate synthase (fpps) are carotenoid and gibberellin pathway genes. Aim of this study was assessing siRNA mediated gene silencing for bikaverin overproduction with down-regulation of carotenoid and gibberel...
Source: Bioresource Technology - February 25, 2014 Category: Biotechnology Authors: Deshmukh R, Purohit HJ Tags: Bioresour Technol Source Type: research

Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown
Nature Biotechnology 33, 870 (2015). doi:10.1038/nbt.3298 Authors: Anders Wittrup, Angela Ai, Xing Liu, Peter Hamar, Radiana Trifonova, Klaus Charisse, Muthiah Manoharan, Tomas Kirchhausen & Judy Lieberman A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient process, we studied the uptake and cytosolic release of siRNAs, formulated in lipoplexes or lipid n...
Source: Nature Biotechnology - July 20, 2015 Category: Biotechnology Authors: Anders WittrupAngela AiXing LiuPeter HamarRadiana TrifonovaKlaus CharisseMuthiah ManoharanTomas KirchhausenJudy Lieberman Tags: Research Source Type: research

Lipid nanoparticle-based co-delivery of epirubicin and BCL-2 siRNA for enhanced intracellular drug release and reversing multidrug resistance.
In this study, an acid-cleavable ketal containing poly (β-amino ester) (KPAE) was used to bind siBCL-2 and the KPAE/siBCL-2 complexes were further coated by epirubicin (EPI) containing lipid to form EPI/siBCL-2 dual loaded lipid-nanoparticles. The results showed that the average size of EPI/siBCL-2-MEND was about 120 nm, and the average zeta potential was about 41 mV. The encapsulation efficiency (EE) of EPI and siBCL-2 was 86.13% and 97.07%, respectively. EPI/siBCL-2 dual loaded lipid-nanoparticles showed enhanced inhibition efficiency than individual EPI-loaded liposomes on HepG2 cells by MTT assay. Moreover, wester...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 12, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Intracellular miRNA or siRNA delivery and function.
Abstract Nanocarrier-mediated delivery and release of short non-coding RNA (e.g., miRNA or siRNA) into the cells with subsequent suppression of the populations of some of the mRNAs and proteins is of interest in the context of the development of a new generation of drugs. Bearing in mind such applications, the author shows the specifics of the corresponding transient kinetics by using three generic models without and with feedback resulting in bistability in the gene expression. In the absence of feedback, the suppression of the mRNA and protein population is transient. In the case of bistable kinetics, non-coding...
Source: Biosystems - May 30, 2018 Category: Biotechnology Authors: Zhdanov VP Tags: Biosystems Source Type: research

A 1,8-naphthalimide-12aneN3 derivative for efficient Cu2+ recognition, lysosome staining and siRNA delivery.
Abstract Development of multifunctional compounds as both fluorescence probes and non-viral vectors is still difficult till date. It is necessary to overcome many hurdles such as the balance of hydrophilic and hydrophobic moieties, binding affinity between multifunctional compound and targeting substrate, the cytotoxicity of multifunctional compound, and so on. In this work, the performances of compound 1 on Cu2+ recognition, lysosome staining and siRNA (small interfering RNA) delivery were investigated. It was found that compound 1 exhibited high selectivity and sensitivity toward Cu2+ in aqueous solutions. The f...
Source: Colloids and Surfaces - October 31, 2019 Category: Biotechnology Authors: Gao YG, Dang K, Zhang WJ, Liu FL, Patil S, Qadir A, Ding AX, Qian AR Tags: Colloids Surf B Biointerfaces Source Type: research

A window onto siRNA delivery
Nature Biotechnology 31, 611 (2013). doi:10.1038/nbt.2634 Authors: Yuhua Wang & Leaf Huang Two studies trace the intracellular trafficking of siRNA delivered by lipid nanoparticles.
Source: Nature Biotechnology - July 9, 2013 Category: Biotechnology Authors: Yuhua WangLeaf Huang Tags: News and Views Source Type: research

Inhibition of Infectious Bursal Disease Virus by Vector Delivered SiRNA in Cell Culture.
In this study, IBDV was isolated from field cases and confirmed by RT-PCR. The virus was then adapted on chicken embryo fibroblast cells (CEF) in which it showed severe cytopathic effects (CPE). The short hairpin RNA (shRNAs) constructs homologous to the VP2 gene were designed and one, having maximum score and fulfilling maximum Reynolds criteria, was selected for evaluation of effective inhibition. Selected shRNA construct (i.e., VP2-shRNA) was observed to be the most effective for inhibiting VP2 gene expression. Real time PCR analysis was performed to measure the relative expression of VP2 gene in different experimental ...
Source: Animal Biotechnology - August 27, 2014 Category: Biotechnology Authors: Sahare AA, Bedekar MK, Jain SK, Singh A, Singh S, Sarkhel BC Tags: Anim Biotechnol Source Type: research

Taking charge of siRNA delivery
Nature Biotechnology 32, 1197 (2014). doi:10.1038/nbt.3091 Authors: Anastasia Khvorova, Maire F Osborn & Matthew R Hassler Delivery of siRNA into cells is achieved by neutralizing the negative charge of the phosphate backbone in a reversible manner.
Source: Nature Biotechnology - December 9, 2014 Category: Biotechnology Authors: Anastasia KhvorovaMaire F OsbornMatthew R Hassler Tags: News and Views Source Type: research