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Total 393 results found since Jan 2013.

Novel cell-penetrating peptide-loaded nanobubbles synergized with ultrasound irradiation enhance EGFR siRNA delivery for triple negative Breast cancer therapy.
Abstract The lack of safe and effective gene delivery strategies remains a bottleneck for cancer gene therapy. Here, we describe the synthesis, characterization, and application of cell-penetrating peptide (CPP)-loaded nanobubbles (NBs), which are characterized by their safety, strong penetrating power and high gene loading capability for gene delivery. An epidermal growth factor receptor (EGFR)-targeted small interfering RNA (siEGFR) was transfected into triple negative breast cancer (TNBC) cells via prepared CPP-NBs synergized with ultrasound-targeted microbubble destruction (UTMD) technology. Fluorescence micro...
Source: Colloids and Surfaces - June 20, 2016 Category: Biotechnology Authors: Jing H, Cheng W, Li S, Wu B, Leng X, Xu S, Tian J Tags: Colloids Surf B Biointerfaces Source Type: research

Local Delivery of CTGF siRNA with Poly(sorbitol-co-PEI) Reduces Scar Contraction in Cutaneous Wound Healing
This study exemplifies a successful treatment of cutaneous wound healing using a polymer system coupled with RNA interference. Hence, the approach holds a great promise for developing new treatments with novel targets in regenerative medicines.
Source: Tissue Engineering and Regenerative Medicine - May 12, 2017 Category: Biotechnology Source Type: research

Host-Guest Interaction-Based Dual response core/shell nanoparticles as efficient siRNA carrier for killing breast cancer cells
Colloids Surf B Biointerfaces. 2021 Jun 11;205:111918. doi: 10.1016/j.colsurfb.2021.111918. Online ahead of print.ABSTRACTHow to overcome multiple obstacles to achieve the efficient and safe delivery of therapeutic genes is still the key to gene therapy. To address this issue, a cationic carrier consisting of polyamide-amine (HPAA-peptide-Fc) modified by an enzyme-responsive polypeptide as the core and hyperbranched polyglycerol derivative (CD-HPG) as the shell was synthesized by self-assembly. The obtained HPAA-peptide-HPG could form the compact nanocomplex with siPlk1, thus confirming the stable load of genes and subsequ...
Source: Colloids and Surfaces - June 18, 2021 Category: Biotechnology Authors: Jinglan Liang Chengguang Wu Xiaoyan Zhou Yunfeng Shi Jun Xu Xiang Cai Tingling Fu Dong Ma Wei Xue Source Type: research

Enhanced sucrose-mediated cryoprotection of siRNA-loaded poly (lactic-co-glycolic acid) nanoparticles
Colloids Surf B Biointerfaces. 2022 Sep 28;220:112880. doi: 10.1016/j.colsurfb.2022.112880. Online ahead of print.ABSTRACTThe present study aimed to determine the effects of sucrose on the physical stability, cellular entry pathways and functional efficacy of poly(lactic-co-glycolic acid) nanoparticles (PLGA-NPs). PLGA-NPs were synthesized in the absence or presence of 10 % sucrose, using HEI-101, an unmodified small interfering RNA (siRNA), as a drug model. The newly synthesized HEI-101-loaded PLGA-NPs (HEI-101-NPs) were exposed to repeated freeze-thaw cycles and iteratively tested over a six-month evaluation period. The ...
Source: Colloids and Surfaces - October 14, 2022 Category: Biotechnology Authors: Ibrahima Youm Matthew B West Xiangping Huang Wei Li Richard D Kopke Source Type: research

Analysis of polymeric nanoparticle properties for siRNA/DNA delivery in a tumor xenograft tissue slice air-liquid interface model
CONCLUSION: The ex vivo 3D tissue slice ALI culture model is a powerful system that allows the effective evaluation of biological nanoparticle efficacy and biocompatibility in an intact tissue environment. It is comparably inexpensive, time-saving, and follows the 3R principle, while allowing the identification of critical nanoparticle properties and optimal candidates for in vivo applications. This article is protected by copyright. All rights reserved.PMID:36541426 | DOI:10.1002/biot.202200415
Source: Biotechnology Journal - December 21, 2022 Category: Biotechnology Authors: Michael Karimov Marlene Scherer Heike Franke Alexander Ewe Achim Aigner Source Type: research