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Total 393 results found since Jan 2013.

KLF16 suppresses human glioma cell proliferation and tumourigenicity by targeting TFAM.
CONCLUSIONS: KLF16 is a key regulator of glioma cell proliferation by directly targeting TFAM. PMID: 29374989 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - January 31, 2018 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Hyperglycemia aggravates microenvironment hypoxia and promotes the metastatic ability of pancreatic cancer
ConclusionOur results demonstrate that the association between hyperglycemia and poor prognosis can be attributed to microenvironment hypoxia in pancreatic cancer.Graphical Abstract
Source: Computational and Structural Biotechnology Journal - October 30, 2018 Category: Biotechnology Source Type: research

Targeting clusterin induces apoptosis, reduces growth ability, invasion and mediates sensitivity to chemotherapy in human cancer in human osteosarcoma cells.
CONCLUSIONS: Overexpression of sCLU was significantly correlated with metastasis and chemosensitivity in osteosarcoma cells. The described sCLU-specific siRNA that can potently silence sCLU gene expression may thus prove valuable agents during antitumor therapy. PMID: 31433751 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - August 20, 2019 Category: Biotechnology Authors: Wang X, Yu Y, Zang L, Zhang P, Ma J, Chen D Tags: Curr Pharm Biotechnol Source Type: research

Molecular and expression characteristics of resistin (RETN) and its effects on the differentiation of intramuscular preadipocyte in goat.
In this study, the expression of RETN mRNA in goat tissues and intramuscular preadipocytes during differentiation was detected by qPCR technique. Furthermore, RNA interference was used to explore the effects of RETN on intramuscular preadipocytes differentiation in goat. The results showed that the cloned goat RETN gene sequence was 428 bp in length, of which the ORF was 330 bp, encoding 109 amino acids. Sequence analysis revealed that it had 12 phosphorylation sites and an O-glycosylation site, and its protein contained a signal peptide sequence. Also, the RETN gene is expressed in goat various analyzed tissues, and t...
Source: Animal Biotechnology - September 12, 2019 Category: Biotechnology Authors: Zhang Y, Wang Y, Xu Q, Zhu J, Lin Y Tags: Anim Biotechnol Source Type: research

PLCG2 promotes hepatocyte proliferation in vitro via NF- κB and ERK pathway by targeting bcl2, myc and ccnd1.
In this study, we investigate the mechanism of PLCG2 action using a short interference RNA (siRNA) method. The effects of PLCG2 on rat liver BRL-3A cells treated siRNA were studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT assay), bromodeoxyuridine (BrdU) labelling assay, flow cytometry method (FCM), quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. The results showed when PLCG2 was reduced, cell vitality and proliferation rate were significantly decreased (p < .05 vs. control). FCM analysis showed that the number of cell division phase (G2 + M) was decl...
Source: Artificial Cells, Nanomedicine and Biotechnology - September 26, 2019 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Activatable MRI-monitoring gene delivery for the theranostic of renal carcinoma.
In this study, we constructed a PEG-MnO2-OPN siRNA nanocomplex via a modular streptavidin bridge for the theranostic treatment of renal carcinoma in vitro and in vivo. This strategy of utilizing PEG-MnO2 nanosheets for OPN siRNA delivery showed effective tumor growth inhibition of 786-O tumor-bearing mice, and such a process could be monitored by the activated T1-weight MRI, illustrating the promising potential of PEG-MnO2 nanosheets for the fabrication of an MRI-based theranostic system. PMID: 31734089 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - November 7, 2019 Category: Biotechnology Authors: Shi M, Wang S, Zheng S, Hou P, Dong L, He M, Wu C, Zhang X, Zuo F, Xu K, Li J Tags: Colloids Surf B Biointerfaces Source Type: research

Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21.
In conclusion, the neuroprotective effects of MEG3 silence on PC12 cells injured by hypoxia were observed in this study. Mechanistically, the neuroprotective effects of MEG3 silence on PC12 cells were via sponging miR-21 and thus regulating PI3K/AKT and NF-κB pathways.HIGHLIGHTSMEG3 is highly expressed in PC12 cells following hypoxic injury;Silence of MEG3 prevents hypoxia-induced cell damage in PC12 cells;MEG3 acts as a miR-21 sponge;MEG3 sponges miR-21 to regulate PI3K/AKT and NF-κB pathways. PMID: 32050796 [PubMed - in process]
Source: Artificial Cells, Nanomedicine and Biotechnology - February 14, 2020 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

A novel vector-based RNAi method using mouse U6 promoter-driven shRNA expression in the filamentous fungus Blakeslea trispora
CONCLUSIONS: The results suggest that the mouse U6 promoter that drives the expression of shRNA vectors may serve as a novel tool for RNAi induction in filamentous fungi.PMID:34185215 | DOI:10.1007/s10529-021-03155-5
Source: Biotechnology Letters - June 29, 2021 Category: Biotechnology Authors: Ye Li Hui Feng Lihua Jin Xiulan Xin Qipeng Yuan Source Type: research

A two-pronged approach to regulate the behaviors of ECs and SMCs by the dual targeting-nanoparticles
Colloids Surf B Biointerfaces. 2021 Aug 26;208:112068. doi: 10.1016/j.colsurfb.2021.112068. Online ahead of print.ABSTRACTInhibiting vascular restenosis remains a tricky challenge for the postoperative development of cardiovascular interventional therapy. The ideal approaches should activate endothelial cells (ECs) and restrain smooth muscle cells (SMCs), however, they are commonly contradictory. Herein, a strategy was developed for synchronizing ECs promotion and SMCs inhibition by codelivery DNA and siRNA for combination therapy. Thus, an easy and efficient strategy integrated dual-superiorities of precise targeting and ...
Source: Colloids and Surfaces - August 31, 2021 Category: Biotechnology Authors: Xiaoyu Wang Bin Gao Xiang-Kui Ren Jintang Guo Shihai Xia Wencheng Zhang Cheng Yang Yakai Feng Source Type: research

Ultrastable pRNA hexameric ring gearing hexameric phi29 DNA-packaging motor by revolving without rotating and coiling.
Abstract Biomotors have previously been classified into two categories: linear and rotational motors. It has long been popularly believed that viral DNA packaging motors are rotation motors. We have recently found that the DNA-packaging motor of bacteriophage phi29 uses a third mechanism: revolution without rotation. phi29 motor consists of three-coaxial rings of hexameric RNA, a hexameric ATPase, and a dodecameric channel. The motor uses six ATP to revolve one helical turn of dsDNA around the hexameric ring of ATPase gp16. Each dodecameric segment tilts at a 30°-angle and runs anti-parallel to the dsDNA helix to...
Source: Current Opinion in Biotechnology - May 14, 2013 Category: Biotechnology Authors: Schwartz C, Guo P Tags: Curr Opin Biotechnol Source Type: research

MiR-424 regulates monocytic differentiation of human leukemia U937 cells by directly targeting CDX2.
Abstract MiR-424 plays an important role via promoting the monocytic differentiation in many human leukemia cell lines. Here, we report that miR-424 decreased miR-125b expression to 36 % by directly targeting caudal type homeobox 2. However, miR-424 also decreased expression of Fes, PU.1 and colony-stimulating factor receptor (MCSFR). As Fes, PU.1 and MCSFR were down-regulated by over-expression of miR-125b (unpublished work), a similar effect of miR-424 and Fes siRNA on CD64, Egr-1, Egr-2 and CEBPA indicates that Fes may be an important downstream target of miR-424. We hypothesize that miR-424 promotes monocytic...
Source: Biotechnology Letters - June 26, 2013 Category: Biotechnology Authors: Shen X, Tang J, Hu J, Guo L, Xing Y, Xi T Tags: Biotechnol Lett Source Type: research

Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape
Nature Biotechnology 31, 638 (2013). doi:10.1038/nbt.2612 Authors: Jerome Gilleron, William Querbes, Anja Zeigerer, Anna Borodovsky, Giovanni Marsico, Undine Schubert, Kevin Manygoats, Sarah Seifert, Cordula Andree, Martin Stöter, Hila Epstein-Barash, Ligang Zhang, Victor Koteliansky, Kevin Fitzgerald, Eugenio Fava, Marc Bickle, Yannis Kalaidzidis, Akin Akinc, Martin Maier & Marino Zerial
Source: Nature Biotechnology - June 23, 2013 Category: Biotechnology Authors: Jerome GilleronWilliam QuerbesAnja ZeigererAnna BorodovskyGiovanni MarsicoUndine SchubertKevin ManygoatsSarah SeifertCordula AndreeMartin StöterHila Epstein-BarashLigang ZhangVictor KotelianskyKevin FitzgeraldEugenio FavaMarc BickleYannis KalaidzidisAkin Tags: Research Source Type: research

SIRT1 Suppresses Activating Transcription Factor 4 (ATF4) Expression in Response to Proteasome Inhibition.
Abstract The synthetic machinery of ATF4 (activating transcription factor 4) is activated in response to various stress conditions involved in nutrient restriction, endoplasmic reticulum homeostasis and oxidation. Stress-induced inhibition of proteasome activity triggers the unfolded protein response and endoplasmic reticulum stress where ATF4 is crucial for consequent biological events. In the current study, we showed that the NAD+-dependent deacetylase, SIRT1, suppresses ATF4 synthesis during proteasome inhibition. SIRT1 depletion via transfection of specific siRNA into HeLa cells resulted in a significant incre...
Source: Journal of Microbiology and Biotechnology - October 4, 2013 Category: Biotechnology Authors: Woo SR, Park JE, Kim YH, Ju YJ, Shin HJ, Joo HY, Park ER, Hong SH, Park GH, Lee KH Tags: J Microbiol Biotechnol Source Type: research

Lithium chloride inhibits TGF-β1-induced myofibroblast transdifferentiation via PI3K/Akt pathway in cultured fibroblasts from Tenon's capsule of the human eye.
Abstract Excess scarring of the conjunctiva after glaucoma filtration surgery is a major cause of failure. Transforming growth factor (TGF)-β is critically involved in post-operative scarring. Lithium inhibits TGF-β-induced gene protein expression in corneal fibroblasts and inhibits TGF-β-induced epithelial mesenchymal transition. Here, we investigated the effects of LiCl on TGF-β1-mediated signaling pathways and on myofibroblast transdifferentiation of human Tenon's capsule fibroblasts (HTFs). LiCl treatment reduced expression of TGF-β1-induced α-SMA expression in HTFs. LiCl also decreased Akt phosphorylati...
Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Chung EJ, Sohn YH, Kwon SH, Jung SA, Lee JH Tags: Biotechnol Lett Source Type: research