Coupling and uncoupling growth and product formation for producing chemicals
Curr Opin Biotechnol. 2024 Apr 18;87:103133. doi: 10.1016/j.copbio.2024.103133. Online ahead of print.ABSTRACTMicrobial fermentation employs two strategies: growth- and nongrowth-coupled productions. Stoichiometric metabolic models with flux balance analysis enable pathway engineering to couple target synthesis with growth, yielding numerous successful results. Growth-coupled engineering also contributes to improving bottleneck flux through subsequent adaptive evolution. However, because growth-coupled production inevitably shares resources between biomass and target syntheses, the cost-effective production of bulk chemica...
Source: Current Opinion in Biotechnology - April 19, 2024 Category: Biotechnology Authors: Yoshihiro Toya Hiroshi Shimizu Source Type: research
Coupling and uncoupling growth and product formation for producing chemicals
Curr Opin Biotechnol. 2024 Apr 18;87:103133. doi: 10.1016/j.copbio.2024.103133. Online ahead of print.ABSTRACTMicrobial fermentation employs two strategies: growth- and nongrowth-coupled productions. Stoichiometric metabolic models with flux balance analysis enable pathway engineering to couple target synthesis with growth, yielding numerous successful results. Growth-coupled engineering also contributes to improving bottleneck flux through subsequent adaptive evolution. However, because growth-coupled production inevitably shares resources between biomass and target syntheses, the cost-effective production of bulk chemica...
Source: Current Opinion in Biotechnology - April 19, 2024 Category: Biotechnology Authors: Yoshihiro Toya Hiroshi Shimizu Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Stem cell engineering approaches for investigating glial cues in central nervous system disorders
Curr Opin Biotechnol. 2024 Apr 9;87:103131. doi: 10.1016/j.copbio.2024.103131. Online ahead of print.ABSTRACTGlial cells are important in maintaining homeostasis for neurons in the central nervous system (CNS). During CNS disease or after injury, glia react to altered microenvironments and often acquire altered functions that contribute to disease pathology. A major focus for research is utilizing stem cell (SC)-derived glia as a potential renewable source for cell replacement to restore function, including neuronal support, and as a model for disease states to identify therapeutic targets. In this review, we focus on SC d...
Source: Current Opinion in Biotechnology - April 10, 2024 Category: Biotechnology Authors: Sangamithra Vardhan Tyler Jordan Shelly Sakiyama-Elbert Source Type: research
Does the surface charge of the nanoparticles drive nanoparticle-cell membrane interactions?
Curr Opin Biotechnol. 2024 Apr 5;87:103128. doi: 10.1016/j.copbio.2024.103128. Online ahead of print.ABSTRACTClassical Coulombic interaction, characterized by electrostatic interactions mediated through surface charges, is often regarded as the primary determinant in nanoparticles' (NPs) cellular association and internalization. However, the intricate physicochemical properties of particle surfaces, biomolecular coronas, and cell surfaces defy this oversimplified perspective. Moreover, the nanometrological techniques employed to characterize NPs in complex physiological fluids often exhibit limited accuracy and reproducibi...
Source: Current Opinion in Biotechnology - April 6, 2024 Category: Biotechnology Authors: Sandor Balog Mauro Sousa de Almeida Patricia Taladriz-Blanco Barbara Rothen-Rutishauser Alke Petri-Fink Source Type: research
Does the surface charge of the nanoparticles drive nanoparticle-cell membrane interactions?
Curr Opin Biotechnol. 2024 Apr 5;87:103128. doi: 10.1016/j.copbio.2024.103128. Online ahead of print.ABSTRACTClassical Coulombic interaction, characterized by electrostatic interactions mediated through surface charges, is often regarded as the primary determinant in nanoparticles' (NPs) cellular association and internalization. However, the intricate physicochemical properties of particle surfaces, biomolecular coronas, and cell surfaces defy this oversimplified perspective. Moreover, the nanometrological techniques employed to characterize NPs in complex physiological fluids often exhibit limited accuracy and reproducibi...
Source: Current Opinion in Biotechnology - April 6, 2024 Category: Biotechnology Authors: Sandor Balog Mauro Sousa de Almeida Patricia Taladriz-Blanco Barbara Rothen-Rutishauser Alke Petri-Fink Source Type: research
Does the surface charge of the nanoparticles drive nanoparticle-cell membrane interactions?
Curr Opin Biotechnol. 2024 Apr 5;87:103128. doi: 10.1016/j.copbio.2024.103128. Online ahead of print.ABSTRACTClassical Coulombic interaction, characterized by electrostatic interactions mediated through surface charges, is often regarded as the primary determinant in nanoparticles' (NPs) cellular association and internalization. However, the intricate physicochemical properties of particle surfaces, biomolecular coronas, and cell surfaces defy this oversimplified perspective. Moreover, the nanometrological techniques employed to characterize NPs in complex physiological fluids often exhibit limited accuracy and reproducibi...
Source: Current Opinion in Biotechnology - April 6, 2024 Category: Biotechnology Authors: Sandor Balog Mauro Sousa de Almeida Patricia Taladriz-Blanco Barbara Rothen-Rutishauser Alke Petri-Fink Source Type: research
Does the surface charge of the nanoparticles drive nanoparticle-cell membrane interactions?
Curr Opin Biotechnol. 2024 Apr 5;87:103128. doi: 10.1016/j.copbio.2024.103128. Online ahead of print.ABSTRACTClassical Coulombic interaction, characterized by electrostatic interactions mediated through surface charges, is often regarded as the primary determinant in nanoparticles' (NPs) cellular association and internalization. However, the intricate physicochemical properties of particle surfaces, biomolecular coronas, and cell surfaces defy this oversimplified perspective. Moreover, the nanometrological techniques employed to characterize NPs in complex physiological fluids often exhibit limited accuracy and reproducibi...
Source: Current Opinion in Biotechnology - April 6, 2024 Category: Biotechnology Authors: Sandor Balog Mauro Sousa de Almeida Patricia Taladriz-Blanco Barbara Rothen-Rutishauser Alke Petri-Fink Source Type: research
Advances in algal lipid metabolism and their use to improve oil content
Curr Opin Biotechnol. 2024 Apr 4;87:103130. doi: 10.1016/j.copbio.2024.103130. Online ahead of print.ABSTRACTMicroalgae are eukaryotic photosynthetic micro-organisms that convert CO2 into carbohydrates, lipids, and other valuable metabolites. They are considered promising chassis for the production of various bioproducts, including fatty acid-derived biofuels. However, algae-based biofuels are not yet commercially available, mainly because of their low yields and high production cost. Optimizing strains to improve lipid productivity using the principles of synthetic biology should help move forward. This necessitates devel...
Source: Current Opinion in Biotechnology - April 5, 2024 Category: Biotechnology Authors: Fantao Kong Carla Blot Keqing Liu Minjae Kim Yonghua Li-Beisson Source Type: research
Advances in algal lipid metabolism and their use to improve oil content
Curr Opin Biotechnol. 2024 Apr 4;87:103130. doi: 10.1016/j.copbio.2024.103130. Online ahead of print.ABSTRACTMicroalgae are eukaryotic photosynthetic micro-organisms that convert CO2 into carbohydrates, lipids, and other valuable metabolites. They are considered promising chassis for the production of various bioproducts, including fatty acid-derived biofuels. However, algae-based biofuels are not yet commercially available, mainly because of their low yields and high production cost. Optimizing strains to improve lipid productivity using the principles of synthetic biology should help move forward. This necessitates devel...
Source: Current Opinion in Biotechnology - April 5, 2024 Category: Biotechnology Authors: Fantao Kong Carla Blot Keqing Liu Minjae Kim Yonghua Li-Beisson Source Type: research
Engineering bacterial warriors: harnessing microbes to modulate animal physiology
Curr Opin Biotechnol. 2024 Apr 1;87:103113. doi: 10.1016/j.copbio.2024.103113. Online ahead of print.ABSTRACTA central goal of synthetic biology is the reprogramming of living systems for predetermined biological functions. While many engineering efforts have been made in living systems, these innovations have been mainly employed with microorganisms or cell lines. The engineering of multicellular organisms including animals remains challenging owing to the complexity of these systems. In this context, microbes, with their intricate impact on animals, have opened new opportunities. Through the utilization of the symbiotic ...
Source: Current Opinion in Biotechnology - April 2, 2024 Category: Biotechnology Authors: Baizhen Gao Daniela Ruiz Hayden Case Robert E Jinkerson Qing Sun Source Type: research
