Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Design, construction, and application of noncanonical redox cofactor infrastructures
Curr Opin Biotechnol. 2023 Nov 6;84:103019. doi: 10.1016/j.copbio.2023.103019. Online ahead of print.ABSTRACTControlling the flow of carbon and reducing power in biological systems is a central theme in metabolic engineering. Often, trade-offs in pushing carbon flux through targeted pathways while operating in conditions agreeable to the host are required due to the central pools of the shared native redox cofactors NAD(P)/H. Noncanonical redox cofactors (NRCs) have emerged as promising tools to transform how engineers develop biotransformation systems. These new-to-Nature redox cofactors have been demonstrated to function...
Source: Current Opinion in Biotechnology - November 8, 2023 Category: Biotechnology Authors: William B Black Sean Perea Han Li Source Type: research
Matching diverse feedstocks to conversion processes for the future bioeconomy
Curr Opin Biotechnol. 2023 Nov 5;84:103017. doi: 10.1016/j.copbio.2023.103017. Online ahead of print.ABSTRACTA wide variety of wasted or underutilized organic feedstocks can be leveraged to build a sustainable bioeconomy, ranging from crop residues to food processor residues and municipal wastes. Leveraging these feedstocks is both high-risk and high-reward. Converting mixed, variable, and/or highly contaminated feedstocks can pose engineering and economic challenges. However, converting these materials to fuels and chemicals can divert waste from landfills, reduce fugitive methane emissions, and enable more responsible fo...
Source: Current Opinion in Biotechnology - November 7, 2023 Category: Biotechnology Authors: Corinne D Scown Nawa R Baral Deepti Tanjore Vi Rapp Source Type: research
Matching diverse feedstocks to conversion processes for the future bioeconomy
Curr Opin Biotechnol. 2023 Nov 5;84:103017. doi: 10.1016/j.copbio.2023.103017. Online ahead of print.ABSTRACTA wide variety of wasted or underutilized organic feedstocks can be leveraged to build a sustainable bioeconomy, ranging from crop residues to food processor residues and municipal wastes. Leveraging these feedstocks is both high-risk and high-reward. Converting mixed, variable, and/or highly contaminated feedstocks can pose engineering and economic challenges. However, converting these materials to fuels and chemicals can divert waste from landfills, reduce fugitive methane emissions, and enable more responsible fo...
Source: Current Opinion in Biotechnology - November 7, 2023 Category: Biotechnology Authors: Corinne D Scown Nawa R Baral Deepti Tanjore Vi Rapp Source Type: research
Advances in generative modeling methods and datasets to design novel enzymes for renewable chemicals and fuels
Curr Opin Biotechnol. 2023 Nov 3;84:103007. doi: 10.1016/j.copbio.2023.103007. Online ahead of print.ABSTRACTBiotechnology has revolutionized the development of sustainable energy sources by harnessing biomass as a feedstock for energy production. However, challenges such as recalcitrant feedstocks and inefficient metabolic pathways hinder the large-scale integration of renewable energy systems. Enzyme engineering has emerged as a powerful tool to address these challenges by enhancing enzyme activity, specificity, and stability. Generative machine learning (ML) models have shown great promise in accelerating protein design...
Source: Current Opinion in Biotechnology - November 6, 2023 Category: Biotechnology Authors: Rana A Barghout Zhiqing Xu Siddharth Betala Radhakrishnan Mahadevan Source Type: research
Advances in generative modeling methods and datasets to design novel enzymes for renewable chemicals and fuels
Curr Opin Biotechnol. 2023 Nov 3;84:103007. doi: 10.1016/j.copbio.2023.103007. Online ahead of print.ABSTRACTBiotechnology has revolutionized the development of sustainable energy sources by harnessing biomass as a feedstock for energy production. However, challenges such as recalcitrant feedstocks and inefficient metabolic pathways hinder the large-scale integration of renewable energy systems. Enzyme engineering has emerged as a powerful tool to address these challenges by enhancing enzyme activity, specificity, and stability. Generative machine learning (ML) models have shown great promise in accelerating protein design...
Source: Current Opinion in Biotechnology - November 6, 2023 Category: Biotechnology Authors: Rana A Barghout Zhiqing Xu Siddharth Betala Radhakrishnan Mahadevan Source Type: research
Editorial overview: Novel approaches to gauge the human footprint on the biosphere
Curr Opin Biotechnol. 2023 Nov 2;84:103018. doi: 10.1016/j.copbio.2023.103018. Online ahead of print.ABSTRACTHuman disturbances are altering the biosphere in unprecedented ways. Yet, the precise picture of how these disturbances are altering the biosphere and the consequences for humans and the planet reamain undefined. The knowledge and tools to quantify these impacts are often dispersed across traditionally independent scientific disciplines. This special issue brings together a large diversity of topics and global experts under the common theme of using novel advances and tools to gauge the human footprint on the biosph...
Source: Current Opinion in Biotechnology - November 4, 2023 Category: Biotechnology Authors: Luis F De Le ón Jaime Martinez-Urtaza Source Type: research
Improving microbial bioproduction under low-oxygen conditions
Curr Opin Biotechnol. 2023 Nov 2;84:103016. doi: 10.1016/j.copbio.2023.103016. Online ahead of print.ABSTRACTMicrobial bioconversion provides access to a wide range of sustainably produced chemicals and commodities. However, industrial-scale bioproduction process operations are preferred to be anaerobic due to the cost associated with oxygen transfer. Anaerobic bioconversion generally offers limited substrate utilization profiles, lower product yields, and reduced final product diversity compared with aerobic processes. Bioproduction under conditions of reduced oxygen can overcome the limitations of fully aerobic and anaer...
Source: Current Opinion in Biotechnology - November 4, 2023 Category: Biotechnology Authors: Shawn Kulakowski Deepanwita Banerjee Corinne D Scown Aindrila Mukhopadhyay Source Type: research
Editorial overview: Novel approaches to gauge the human footprint on the biosphere
Curr Opin Biotechnol. 2023 Nov 2;84:103018. doi: 10.1016/j.copbio.2023.103018. Online ahead of print.ABSTRACTHuman disturbances are altering the biosphere in unprecedented ways. Yet, the precise picture of how these disturbances are altering the biosphere and the consequences for humans and the planet reamain undefined. The knowledge and tools to quantify these impacts are often dispersed across traditionally independent scientific disciplines. This special issue brings together a large diversity of topics and global experts under the common theme of using novel advances and tools to gauge the human footprint on the biosph...
Source: Current Opinion in Biotechnology - November 4, 2023 Category: Biotechnology Authors: Luis F De Le ón Jaime Martinez-Urtaza Source Type: research
Improving microbial bioproduction under low-oxygen conditions
Curr Opin Biotechnol. 2023 Nov 2;84:103016. doi: 10.1016/j.copbio.2023.103016. Online ahead of print.ABSTRACTMicrobial bioconversion provides access to a wide range of sustainably produced chemicals and commodities. However, industrial-scale bioproduction process operations are preferred to be anaerobic due to the cost associated with oxygen transfer. Anaerobic bioconversion generally offers limited substrate utilization profiles, lower product yields, and reduced final product diversity compared with aerobic processes. Bioproduction under conditions of reduced oxygen can overcome the limitations of fully aerobic and anaer...
Source: Current Opinion in Biotechnology - November 4, 2023 Category: Biotechnology Authors: Shawn Kulakowski Deepanwita Banerjee Corinne D Scown Aindrila Mukhopadhyay Source Type: research
Emerging nonmodel eukaryotes for biofuel production
Curr Opin Biotechnol. 2023 Oct 30;84:103015. doi: 10.1016/j.copbio.2023.103015. Online ahead of print.ABSTRACTMicrobial synthesis of biofuels offers a promising solution to the global environmental and energy concerns. However, the main challenge of microbial cell factories is their high fermentation costs. Model hosts, such as Escherichia coli and Saccharomyces cerevisiae, are typically used for proof-of-concept studies of producing different types of biofuels, however, they have a limited potential for biofuel production at an industrially relevant scale due to the weak stability/robustness and narrow substrate scope. Wi...
Source: Current Opinion in Biotechnology - November 1, 2023 Category: Biotechnology Authors: Lin Hu Huihui Qiu Liuheng Huang Fenghui Zhang Vinh G Tran Jifeng Yuan Ning He Mingfeng Cao Source Type: research
