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Total 393 results found since Jan 2013.

Mesoporous silica nanoparticle-modified electrode arrays of cochlear implants for delivery of siRNA-TGF β1 into the inner ear
Colloids Surf B Biointerfaces. 2022 Aug 4;218:112753. doi: 10.1016/j.colsurfb.2022.112753. Online ahead of print.ABSTRACTCochlear implants (CI) are widely used in patients to restore hearing function. Uncontrolled fibrosis in the cochleae induced by excess secretion of TGFβ1 seriously affects the effectiveness of CIs. siRNA is a potential therapeutic strategy to downregulate TGFβ1 specifically. However, treatment with siRNA in cochleae is difficult due to the poor penetration capability and instability of siRNA and the inaccessibility and vulnerability of cochleae. To address these challenges, we developed amino-function...
Source: Colloids and Surfaces - August 13, 2022 Category: Biotechnology Authors: Anning Chen Yaoheng Chen Shixin Liu Dong Ma Jie Tang Hongzheng Zhang Source Type: research

Polymeric nanoparticles-siRNA as an emerging nano-polyplexes against ovarian cancer
Colloids Surf B Biointerfaces. 2022 Aug 9;218:112766. doi: 10.1016/j.colsurfb.2022.112766. Online ahead of print.ABSTRACTOvarian cancer (OC) is considered fifth-deadliest cancer globally responsible for high mortality in women. As the conventional therapeutic and diagnostic approaches are ineffective in increasing the survival rates of advanced staged patients by more than 5 years, OC has resulted in high morbidity and mortality rates over the last two decades. As a result, there is a dire need for innovative treatment approaches to address the issues. RNAi and nanotechnology can be considered the most appropriate strategi...
Source: Colloids and Surfaces - August 22, 2022 Category: Biotechnology Authors: Urushi Rehman Neha Parveen Afsana Sheikh Mohammed A S Abourehab Amirhossein Sahebkar Prashant Kesharwani Source Type: research

Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling
Nature Biotechnology 31, 653 (2013). doi:10.1038/nbt.2614 Authors: Gaurav Sahay, William Querbes, Christopher Alabi, Ahmed Eltoukhy, Sovan Sarkar, Christopher Zurenko, Emmanouil Karagiannis, Kevin Love, Delai Chen, Roberto Zoncu, Yosef Buganim, Avi Schroeder, Robert Langer & Daniel G Anderson Despite efforts to understand the interactions between nanoparticles and cells, the cellular processes that determine the efficiency of intracellular drug delivery remain unclear. Here we examine cellular uptake of short interfering RNA (siRNA) delivered in lipid nanoparticles (LNPs) using cellular trafficking probes in co...
Source: Nature Biotechnology - June 23, 2013 Category: Biotechnology Authors: Gaurav SahayWilliam QuerbesChristopher AlabiAhmed EltoukhySovan SarkarChristopher ZurenkoEmmanouil KaragiannisKevin LoveDelai ChenRoberto ZoncuYosef BuganimAvi SchroederRobert LangerDaniel G Anderson Tags: Research Source Type: research

Silencing of the scavenger receptor (Class B - Type 1) gene using siRNA-loaded chitosan nanaoparticles in a HepG2 cell model.
In this study, we investigated the use of chitosan nanoparticles as non-viral delivery carriers of siRNA. As a model target, we selected the scavenger receptor (SR-B1), due to its proposed involvement in hepatitis C virus (HCV) internalization. Low molecular weight (LMW) chitosan nanoparticles were prepared by simple ionic gelation using sodium tripolyphosphate (TPP) as a cross-linking agent; a fixed chitosan and TPP concentration of 0.1% was used, and a chitosan to TPP weight ratios of 3:1, 5:1, and 9:1 were investigated. Nanoparticle uptake efficiency was measured using FITC-labeled chitosan nanoparticles and silencing o...
Source: Colloids and Surfaces - November 3, 2014 Category: Biotechnology Authors: Farid MM, Hathout RM, Fawzy M, Abou-Aisha K Tags: Colloids Surf B Biointerfaces Source Type: research

Mechanisms of immune system activation in mammalians by small interfering RNA (siRNA).
Authors: Mansoori B, Mohammadi A, Shir Jang S, Baradaran B Abstract RNA interference (RNAi) guided by small interfering RNAs (siRNA), because of its potential to target and silence the expression of specific genes is utilized as an effective tool in a variety of biological applications. RNAi guided by siRNAs is a powerful tool to attain gene silencing in mammalian cells. One of the features which make siRNA as an amazing biological tool is extremely specific knockdown of target genes by degradation of analogous mRNAs. However, various non-specific effects limit the use of RNAi including the activation of innate imm...
Source: Artificial Cells, Nanomedicine and Biotechnology - December 12, 2015 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

The impact of the codelivery of drug-siRNA by trimethyl chitosan nanoparticles on the efficacy of chemotherapy for metastatic breast cancer cell line (MDA-MB-231).
Authors: Eivazy P, Atyabi F, Jadidi-Niaragh F, Aghebati Maleki L, Miahipour A, Abdolalizadeh J, Yousefi M Abstract High-mobility group protein two (HMGA2), a nonhistone nuclear-binding protein and its downregulators; vimentin, matrix metallopeptidase-9 (MMP-9), and E-cadherin are shown to contribute to tumor progression and metastasis. Thus, in this study, we checked simultaneous delivery of HMGA-2 siRNA and the anticancer drug doxorubicin to enhance the anticancer treatment effects. For this purpose, we used MTT assay and real-time polymerase chain reaction (RT-PCR). Our results showed that dual delivery of Dox an...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 19, 2016 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

SiRNA-mediated silencing of Snail-1 induces apoptosis and alters micro RNA expression in human urinary bladder cancer cell line.
Authors: Musavi Shenas SM, Mansoori B, Mohammadi A, Salehi S, Kaffash B, Talebi B, Babaloo Z, Shanehbandi D, Baradaran B Abstract Snail-1 known as one of the important transcription factor is a mediator of survival and cell migration, and expression is raised in numerous cancer types. Snail-1 gene may show a role in recurrence of several cancers including bladder cancer by down-regulating E-cadherin, inducing an epithelial to mesenchymal transition (EMT) and its related microRNAs (miRNAs). The aim of this study was to investigate the effect of a specific Snail-1 siRNA on apoptosis and alter EMT related miRNAs of EJ...
Source: Artificial Cells, Nanomedicine and Biotechnology - June 22, 2016 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Co-delivery of insulin-like growth factor 1 receptor specific siRNA and doxorubicin using chitosan-based nanoparticles enhanced anticancer efficacy in A549 lung cancer cell line.
Authors: Shali H, Shabani M, Pourgholi F, Hajivalili M, Aghebati-Maleki L, Jadidi-Niaragh F, Baradaran B, Movassaghpour Akbari AA, Younesi V, Yousefi M Abstract Here, we investigated the effects of dual delivery of IGF-1R siRNA and doxorubicin by chitosan nanoparticles on viability of A549 lung cancer cells line by utilization of MTT and qRT-PCR. Furthermore apoptosis and migration of treated cells were assessed by Annexin-PI and wound healing assays, respectively. The chitosan nanoparticles had about 176 nm size with zeta potential and polydispersive index about 11 mV and 0.3, respectively. The IGF-1R siRNA ha...
Source: Artificial Cells, Nanomedicine and Biotechnology - April 2, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Toxicological exploration of peptide-based cationic liposomes in siRNA delivery.
Abstract The toxicology of cationic liposomes was explored to advance clinical trials of liposome-mediated gene therapy through the analysis of a peptide cationic liposome with DOTAP as a positive control. We first investigated the delivery of luciferase siRNA by several peptide liposomes in mice bearing lung cancer A549 cell xenografts. Of these, a cationic liposome (CDO14) was selected for further investigation. CDO14 efficiently mediated IGF-1R-siRNA delivery and inhibited the growth of the A549 cell xenografts. The in vivo toxicity and toxicological mechanisms of the selected liposome were evaluated to assess ...
Source: Colloids and Surfaces - March 24, 2019 Category: Biotechnology Authors: Zhu Y, Meng Y, Zhao Y, Zhu J, Xu H, Zhang E, Shi L, Du L, Liu G, Zhang C, Xu X, Kang X, Zhen Y, Zhang S Tags: Colloids Surf B Biointerfaces Source Type: research

Lipid-based Vehicles for siRNA Delivery in Biomedical Field.
Abstract Genetic drugs have aroused much attention in the past twenty years. RNA interference (RNAi) offers novel insights into discovering potential gene functions and therapies targeting genetic diseases. Small interference RNA (siRNA), typically 21-23 nucleotides in length, can specifically degrade complementary mRNA. However, targeted delivery and controlled release of siRNA remain a great challenge. Considering the vast number of drug carriers, when compared with viral vectors, non-viral ones show reduced cytotoxicity and low production cost. Especially, lipid-based vehicles are more promising due to their hi...
Source: Current Pharmaceutical Biotechnology - September 23, 2019 Category: Biotechnology Authors: Li T, Huang L, Yang M Tags: Curr Pharm Biotechnol Source Type: research

Recent advances in siRNA delivery systems for prostate cancer therapy
Curr Pharm Biotechnol. 2021 Jun 15. doi: 10.2174/1389201022666210615123211. Online ahead of print.ABSTRACTThe critical problems of conventional prostate cancer therapeutic strategies like nonspecific toxicity and multi-drug resistance prompted the development and application of countless nanoparticle-based siRNA therapeutics. The main challenges to siRNA-based therapeutics becoming a new paradigm in the treatment of prostate cancer stem from the lack of safe and effective delivery systems, immune system stimulation, poor knowledge of nano-bio interactions, and limitations concerning designing, manufacturing, clinical trans...
Source: Current Pharmaceutical Biotechnology - June 16, 2021 Category: Biotechnology Authors: Shahin Aghamiri Pourya Raee Shiva Shahmohamadnejad Sasan Shabani Jaber Ghorbani Marzieh Sameni Mohammad Taha Ebrahimi Source Type: research