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Total 393 results found since Jan 2013.

< em > Chelidonium majus < /em > Induces Apoptosis of Human Ovarian Cancer Cells Via ATF3-Mediated Regulation of Foxo3a by Tip60
This study demonstrated that Chelidonium majus upregulated the expression of ATF3 and Tip60 and promoted Foxo3a nuclear translocation, ultimately increasing the level of Bcl-2-associated X protein (Bax) protein. ATF3 overexpression stimulated Tip60 expression, while ATF3 inhibition by siRNA repressed Tip60 expression. Furthermore, siRNA-mediated Tip60 inhibition significantly promoted Foxo3a phosphorylation, leading to blockade of Foxo3a translocation into the nucleus. Thus, we were able to deduce that ATF3 mediates the regulation of Foxo3a by Tip60. Moreover, siRNA-mediated Foxo3a inhibition suppressed the expression of B...
Source: Journal of Microbiology and Biotechnology - March 14, 2022 Category: Biotechnology Authors: Lei Shen Soon Lee Jong Cheon Joo Eunmi Hong Zhen Yang Cui Eunbi Jo Soo Jung Park Hyun-Jin Jang Source Type: research

A cationic amino acid polymer nanocarrier synthesized in supercritical CO < sub > 2 < /sub > for co-delivery of drug and gene to cervical cancer cells
Colloids Surf B Biointerfaces. 2022 May 18;216:112584. doi: 10.1016/j.colsurfb.2022.112584. Online ahead of print.ABSTRACTThe present study was undertaken to investigate the ability of a drug curcumin-loaded polymer to inhibit the growth of cervical cancer cells by enhancing the anti-cancer efficiency of curcumin. We synthesized poly(methacryloyl beta-alanine) (PMBA) as a nanocarrier by radical polymerization in supercritical CO2. The results showed that the curcumin encapsulated and folic acid (FA)-treated PMBA (Poly@Cur-FA) for 24 h activated the reactive oxygen species-mediated programmed cell death machinery in HeLa ce...
Source: Colloids and Surfaces - May 26, 2022 Category: Biotechnology Authors: K V Kavya Stella Vargheese Shruti Shukla Imran Khan Debasish Kumar Dey Vivek K Bajpai Kavitha Thangavelu Raju Vivek R T Rajendra Kumar Young-Kyu Han Yun Suk Huh Yuvaraj Haldorai Source Type: research

Efficient and specific gene knockdown by small interfering RNAs produced in bacteria
Nature Biotechnology 31, 350 (2013). doi:10.1038/nbt.2537 Authors: Linfeng Huang, Jingmin Jin, Padraig Deighan, Evgeny Kiner, Larry McReynolds & Judy Lieberman Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. Whe...
Source: Nature Biotechnology - March 10, 2013 Category: Biotechnology Authors: Linfeng HuangJingmin JinPadraig DeighanEvgeny KinerLarry McReynoldsJudy Lieberman Tags: Research Source Type: research

Knockdown of PsbO leads to induction of HydA and production of photobiological H2 in the green alga Chlorella sp. DT.
Abstract Green algae are able to convert solar energy to H2 via the photosynthetic electron transport pathway under certain conditions. Algal hydrogenase (HydA, encoded by HYDA) is in charge of catalyzing the reaction: 2H(+)+2e(-)↔H2 but usually inhibited by O2, a byproduct of photosynthesis. The aim of this study was to knockdown PsbO (encoded by psbO), a subunit concerned with O2 evolution, so that it would lead to HydA induction. The alga, Chlorella sp. DT, was then transformed with short interference RNA antisense-psbO (siRNA-psbO) fragments. The algal mutants were selected by checking for the existence of s...
Source: Bioresource Technology - June 4, 2013 Category: Biotechnology Authors: Lin HD, Liu BH, Kuo TT, Tsai HC, Feng TY, Huang CC, Chien LF Tags: Bioresour Technol Source Type: research

Tristetraprolin regulates prostate cancer cell growth through suppression of E2F1.
Abstract The transcription factor E2F1 is active during the G1 to S transition and is involved in cell cycle and progression. A recent study reported that increased E2F1 is associated with DNA damage and tumour development in several tissues using transgenic models. Here, we show that E2F1 expression is regulated by tristetraprolin (TTP) in prostate cancer. Overexpression of TTP decreased the stability of E2F1 mRNA and the expression level of E2F1. In contrast, inhibition of TTP using siRNA increased the E2F1 expression. E2F1 mRNA contains three AREs within the 3'UTR and TTP destabilized a luciferase mRNA that con...
Source: Journal of Microbiology and Biotechnology - October 22, 2013 Category: Biotechnology Authors: Lee HH, Lee SR, Leem SH Tags: J Microbiol Biotechnol Source Type: research

Heat-shock protein beta 1 regulates androgen-mediated bovine myogenesis.
Abstract To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) and repressed the formation of myotubes in cells induced for myogenic differentiation. In contrast, overexpression of HSPB1 enhanced the expression of desmin and accelerated formation of myotubes. The loss and gain of HSPB1 function was closely associated with the expression level of androgen...
Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Zhang Q, Lee HG, Kang SK, Baik M, Choi YJ Tags: Biotechnol Lett Source Type: research

siRNAs encapsulated in Recombinant Capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells
In this study we have evaluated the utility of the protein as an siRNA carrier for anti Dengue viral and anti cancer applications using cell culture systems. We show that target specific siRNAs delivered using the capsid protein inhibit infection by the four serotypes of Dengue virus and proliferation of two cancer cell lines. Our data confirm the potential of the capsid for anti Dengue viral and anti cancer RNAi applications. In addition, we have optimized a fermentation strategy to improve the yield of E. coli expressed D2C protein since the reported yield of E.coli expressed flaviviral capsid proteins are low.
Source: Journal of Biotechnology - November 13, 2014 Category: Biotechnology Source Type: research

Retinoblastoma-binding protein 2 induces epithelial-mesenchymal transition in esophageal squamous cancer cells.
CONCLUSION: RBP2 could induce EMT in esophageal cancer cells and exert a greater effect on the expression of E-cadherin in lung squamous cells than in esophageal squamous cells. PMID: 26264242 [PubMed - as supplied by publisher]
Source: Biotechnology Letters - August 12, 2015 Category: Biotechnology Authors: Qiao N, Wang S, Hu L Tags: Biotechnol Lett Source Type: research

Chitosan-Mediated shRNA Knockdown of Cytosolic Alanine Aminotransferase Improves Hepatic Carbohydrate Metabolism.
In this study, we examined the effect of silencing cytosolic ALT (cALT) expression on the hepatic metabolism in Sparus aurata. A number of siRNA and shRNA designed to down-regulate cALT expression were validated in HEK-293 cells transfected with plasmids expressing S. aurata cALT or mitochondrial ALT (mALT) isoforms: ALT silencing significantly decreased the expression levels of S. aurata mRNA cALT1 to 62 % (siRNA) and 48 % (shRNA) of the values observed in control cells. The effect of cALT silencing was analysed in the liver of S. aurata 72 h after intraperitoneal injection of chitosan-tripolyphosphate (TPP) nanopartic...
Source: Marine Biotechnology - October 16, 2015 Category: Biotechnology Authors: González JD, Silva-Marrero JI, Metón I, Caballero-Solares A, Viegas I, Fernández F, Miñarro M, Fàbregas A, Ticó JR, Jones JG, Baanante IV Tags: Mar Biotechnol (NY) Source Type: research

EGb 761 protects cardiac microvascular endothelial cells against hypoxia/reoxygenation injury and exerts inhibitory effect on ATM pathway.
In this study, H/R-injuried MVECs were treated with EGb 761, then cell viability, apoptosis, ROS production, SOD activity, caspase-3 activity, and the protein level of ATM, γ-H2AX, p53, Bax were measured. ATM siRNA was transfected to study the changes of protein in ATM pathway. EGb 761 presented protective effect on H/R-injuried MVECs with decreasing cell death, apoptosis and ROS, and elevated SOD activity. Next, EGb 761 could inhibit H/R-induced ATM, γ-H2AX, p53, Bax in a dose-dependent manner. Moreover, ATM siRNA also could inhibit H/R-induced ATM, γ-H2AX, p53, Bax. Overall, these findings verify EGb 761 protects card...
Source: Journal of Microbiology and Biotechnology - December 13, 2016 Category: Biotechnology Authors: Zhang C, Wang DF, Zhang Z, Han D, Yang K Tags: J Microbiol Biotechnol Source Type: research

Liposome-mediated RNA interference delivery against Erk1 and Erk2 does not equally promote chemosensitivity in human hepatocellular carcinoma cell line HepG2.
CONCLUSION: Our data show that liposomal siRNA-mediated down-regulation of Erk1/2 can lead to potent chemosensitizing effects in HepG2 cells. Moreover, a higher chemosensitivity following Erk2 down-regulation than Erk1 down-regulation may be associated with the higher expression of Erk2 in human HCC. PMID: 28058860 [PubMed - as supplied by publisher]
Source: Artificial Cells, Nanomedicine and Biotechnology - January 7, 2017 Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research

Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions.
Abstract The interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane dendrimers of generations 1-3 with two different ammonium functions at the periphery ([-NMe2R]+, R=Me, (CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single molecule pulling experiments) and molecular dynamic calculations. These studies state the presence of different forces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higher dendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compounds...
Source: Colloids and Surfaces - December 8, 2017 Category: Biotechnology Authors: Sánchez-Milla M, Pastor I, Maly M, Serramía MJ, Gómez R, Sánchez-Nieves J, Ritort F, Muñoz-Fernández MÁ, de la Mata FJ Tags: Colloids Surf B Biointerfaces Source Type: research