Heat-shock protein beta 1 regulates androgen-mediated bovine myogenesis.

Heat-shock protein beta 1 regulates androgen-mediated bovine myogenesis.
Biotechnol Lett. 2014 Feb 22;
Authors: Zhang Q, Lee HG, Kang SK, Baik M, Choi YJ
Abstract
To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) and repressed the formation of myotubes in cells induced for myogenic differentiation. In contrast, overexpression of HSPB1 enhanced the expression of desmin and accelerated formation of myotubes. The loss and gain of HSPB1 function was closely associated with the expression level of androgen receptor (AR). Our findings suggest that HSPB1 mediates androgen signaling by binding directly to AR and then enhancing androgen-mediated myogenesis in myogenic cells.
PMID: 24563320 [PubMed - as supplied by publisher]

Source: Biotechnology Letters - February 22, 2014 Category: Biotechnology Authors: Zhang Q, Lee HG, Kang SK, Baik M, Choi YJ Tags: Biotechnol Lett Source Type: research