Thermoresponsive and Biodegradable Amphiphilic Block Copolymers with Pendant Functional Groups
CONCLUSION:MCL-(CO), MCL-(COOH), and MCL-(NH2) with ketone, COOH, and NH2 pendant groups showed solution-to-hydrogel phase transitions and biodegradation behaviors that depended on both the type and number of pendant groups. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 4, 2018 Category: Biotechnology Source Type: research

A Novel Dorsal Slit Approached Non-Ischemic Partial Nephrectomy Method for a Renal Tissue Regeneration in a Mouse Model
CONCLUSION:The Non-IR and dorsal slit method for kidney surgery in a mouse model can be an alternative surgical approach for researchers without adverse reactions such as apoptosis, inflammation, fibrosis, functional impairment, and systemic reactions. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 1, 2018 Category: Biotechnology Source Type: research

In Vitro and In Vivo Osteogenesis of Human Orbicularis Oculi Muscle-Derived Stem Cells
CONCLUSION:Our data suggest that human OOM tissue is a valuable and noninvasive resource for osteoprogenitor cells to be used in bone repair and regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 29, 2018 Category: Biotechnology Source Type: research

A Pathophysiological Validation of Collagenase II-Induced Biochemical Osteoarthritis Animal Model in Rabbit
CONCLUSION:These study shows that biochemically induced cartilage degeneration truly represented uniform and reliable OA state. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 29, 2018 Category: Biotechnology Source Type: research

Correction to: Endogenous Stem Cells in Homeostasis and Aging
Regrettably, in the previous version of this article, two important references ([47-2, 173] were missing. The authors would like to make following corrections in the original version of the article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 19, 2018 Category: Biotechnology Source Type: research

MiR-214 Regulates the Human Hair Follicle Stem Cell Proliferation and Differentiation by Targeting EZH2 and Wnt/ β-Catenin Signaling Way In Vitro
AbstractmiR-214 plays a major role in the self-renewal of skin tissue. However, whether miR-214 regulates the proliferation and differentiation of human hair follicle stem cells (HFSCs) is unknown. Primary HFSCs were isolated from human scalp skin tissue, cultured, and identified using flow cytometry. An miR-214 mimic and inhibitor were constructed for transfection into HFSCs. The MTS and colony formation assays examined cell proliferation. Immunofluorescence detected the localization and expression levels of TCF4, β-catenin, and differentiation markers. Luciferase reporter and TOP/FOP Flash assays investigated whethe...
Source: Tissue Engineering and Regenerative Medicine - May 7, 2018 Category: Biotechnology Source Type: research

In Vitro Anti-Inflammation and Chondrogenic Differentiation Effects of Inclusion Nanocomplexes of Hyaluronic Acid-Beta Cyclodextrin and Simvastatin
AbstractThe aim of this study was to prepare inclusion nanocomplexes of hyaluronic acid- β-cyclodextrin and simvastatin (HA-β-CD/SIM) and evaluatein vitro anti-inflammation effects on lipopolysaccharide (LPS)-activated synoviocytes and chondrogenic differentiation effects on rat adipose-derived stem cells (rADSCs). The β-CD moieties in HA-β-CD could incorporate SIM to form HA-β-CD/SIM nanocomplexes with diameters of 297–350 nm. HA-β-CD/SIM resulted in long-term release of SIM from the nanocomplexes for up to 63 days in a sustained manner.In vitro studies revealed that HA- &beta...
Source: Tissue Engineering and Regenerative Medicine - May 3, 2018 Category: Biotechnology Source Type: research

Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography
This study evaluated the feasibility of using micro-computed tomography ( µCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffoldsin vitro. Engineered cartilages were incubated with Hexabrix 320 for 20  min and analyzed via µCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D µCT images of...
Source: Tissue Engineering and Regenerative Medicine - April 25, 2018 Category: Biotechnology Source Type: research

Remifentanil Negatively Regulates RANKL-Induced Osteoclast Differentiation and Bone Resorption by Inhibiting c-Fos/NFATc1 Expression
This study shows that remifentanil inhibits the differentiation and maturation of osteoclasts and reduces bone resorption. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - April 21, 2018 Category: Biotechnology Source Type: research

Schwann Cells Enhance Penetration of Regenerated Axons into Three-Dimensional Microchannels
AbstractNerve regeneration after injury requires proper axon alignment to bridge the lesion site and myelination to achieve functional recovery. Transplanted scaffolds with aligned channels, have been shown to induce axon growth to some extent. However, the penetration of axons into the microchannels remain a challenge, influencing the functional recovery of regenerated nerves. We previously demonstrated that the size of microchannels exerts significant impact on Schwann cells (SCs) migration. Here we demonstrate that migration of SCs promotes, significantly, the dorsal root ganglion (DRG) neurons to extend axons into thre...
Source: Tissue Engineering and Regenerative Medicine - February 28, 2018 Category: Biotechnology Source Type: research

In Vivo Biocompatibility and Improved Compression Strength of Reinforced Keratin/Hydroxyapatite Scaffold
AbstractA rapid freezing/lyophilizing/reinforcing process is suggested to fabricate reinforced keratin/hydroxyapatite (HA) scaffold with improved mechanical property and biocompatibility for tissue engineering. The keratin, extracted from human hair, and HA mixture were rapidly frozen with liquid nitrogen and then lyophilized to prepare keratin/HA laminar scaffold. The scaffold was then immersed in PBS for reinforcement treatment, and followed by a second lyophilization to prepare the reinforced keratin/HA scaffold. The morphology, mechanical, chemical, crystal and thermal property of the keratin/HA scaffold were investiga...
Source: Tissue Engineering and Regenerative Medicine - February 23, 2018 Category: Biotechnology Source Type: research

A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
AbstractChronic kidney disease is a major global health problem affecting millions of people; kidney tissue engineering provides an opportunity to better understand this disease, and has the capacity to provide a cure. Two-dimensional cell culture and decellularised tissue have been the main focus of this research thus far, but despite promising results these methods are not without their shortcomings. Polymer fabrication techniques such as electrospinning have the potential to provide a non-woven path for kidney tissue engineering. In this experiment we isolated rat primary kidney cells which were seeded on electrospun po...
Source: Tissue Engineering and Regenerative Medicine - February 14, 2018 Category: Biotechnology Source Type: research

Mechanically Reinforced Extracellular Matrix Scaffold for Application of Cartilage Tissue Engineering
In this study, decellularized porcine cartilage-derived extracellular matrix (ECM) was utilized to fabricate ECM scaffolds. Mechanically reinforced ECM scaffolds were developed by combining salt-leaching and crosslinking for cartilage repair. The developed scaffolds were investigated with respect to their physicochemical properties and their cartilage tissue formation ability. The mechanically reinforced ECM scaffold showed similar mechanical strength to that of synthetic PLGA scaffold and expressed higher levels of cartilage-specific markers compared to those expressed by the ECM scaffold prepared by simple freeze-drying....
Source: Tissue Engineering and Regenerative Medicine - February 8, 2018 Category: Biotechnology Source Type: research

In Vivo Observation of Endothelial Cell-Assisted Vascularization in Pancreatic Cancer Xenograft Engineering
In this study, for better understanding of patient-derived xenograft (PDX) generation, angiogenic characteristics during PDX cancerous tissue generation was investigated with different initial cell seeding conditions in the hydrogel. We monitored the angiogenic changes during the formation ofin vivo cancer cell line xenografts induced by endothelial cells. Ourin vivo cancer tissue formation system was designed with the assistance of tissue engineering technology to mimic patient-derived xenograft formation. Endothelial cells and MIA PaCa-2 pancreatic carcinoma cells were encapsulated in fibrin gel at different mixing confi...
Source: Tissue Engineering and Regenerative Medicine - February 3, 2018 Category: Biotechnology Source Type: research

Combined Treatment with Methylprednisolone and Human Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Experimental Autoimmune Encephalomyelitis
AbstractMultiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Although advances have been made in the treatment of MS, such as the use of IFN- β, glucocorticoids and stem cells, the therapeutic effects of these treatments are not sufficient. In the present study, we evaluated whether the combination of methylprednisolone (MP) and human bone marrow-derived mesenchymal stem cells (BM-MSCs) could enhance the therapeutic effectiveness in expe rimental autoimmune encephalomyelitis (EAE), a model for MS. EAE was induced by immunizing C57BL/6 mice with myelin oligodendrocyte glycopro...
Source: Tissue Engineering and Regenerative Medicine - January 25, 2018 Category: Biotechnology Source Type: research

Correction to: New Advances in Human X Chromosome Status from a Developmental and Stem Cell Biology
AbstractUnfortunately, Acknowledgements section was missing in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Correction to: Engineered M13 Nanofiber Accelerates Ischemic Neovascularization by Enhancing Endothelial Progenitor Cells
AbstractThere is a minor spelling error in the last of name of the 9th author in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Osteogenic Nanofibrous Coated Titanium Implant Results in Enhanced Osseointegration: In Vivo Preliminary Study in a Rabbit Model
AbstractA titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and hence results in enhanced osseointegration of the implant. The osteogenic nanofibrous coating (ONFC) consists of poly-caprolactone, gelatin, nano-sized hydroxyapatite, dexamethasone, ascorbic acid and beta-glycerophosphate. ONFC exhibits optimum mechanical properties to support mesenchymal stem cells and...
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Correction to: Extracellular Vesicles in Neurodegenerative Diseases: A Double-Edged Sword
AbstractUnfortunately, Acknowledgements section was missing in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 18, 2018 Category: Biotechnology Source Type: research

Adipose Stem Cells with Conditioned Media for Treatment of Acne Vulgaris Scar
In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the C M has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Feeder Cells Free Rabbit Oral Mucosa Epithelial Cell Sheet Engineering
AbstractThe optimal cell culture method of autologous oral mucosal epithelial cell sheet is not well established for a safe transplantation on to the patients ’ ocular surface. Animal serum and 3T3 mouse feeder cells are currently being used to stimulate the growth of the epithelial cells. However, the use of animal compounds can have potential side effects for the patient after transplantation of the engineered cell sheet. In the present study, we focu sed on engineering a rabbit oral mucosal epithelial cell sheet without 3T3 mouse feeder cells using a mix of Dulbecco’s Modified Eagle Medium/Bronchial Epitheli...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Stem Cell Tracing Through MR Molecular Imaging
AbstractStem cell therapy opens a new window in medicine to overcome several diseases that remain incurable. It appears such diseases as cardiovascular disorders, brain injury, multiple sclerosis, urinary system diseases, cartilage lesions and diabetes are curable with stem cell transplantation. However, some questions related to stem cell therapy have remained unanswered. Stem cell imaging allows approval of appropriated strategies such as selection of the type and dose of stem cell, and also mode of cell delivery before being tested in clinical trials. MRI as a non-invasive imaging modality provides proper conditions for...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach
AbstractSilk fibroin/xanthan composite was investigated as a suitable biomedical material for controlled drug delivery, and blending ratios of silk fibroin and xanthan were optimized by response surface methodology (RSM) and artificial neural network (ANN) approach. A non-linear ANN model was developed to predict the effect of blending ratios, percentage swelling and porosity of composite material on cumulative percentage release. The efficiency of RSM was assessed against ANN and it was found that ANN is better in optimizing and modeling studies for the fabrication of the composite material.In-vitro release studies of the...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Effects of Hexachlorophene, a Chemical Accumulating in Adipose Tissue, on Mouse and Human Mesenchymal Stem Cells
AbstractThe hexachlorophene (HCP) is a highly lipophilic chlorinated bisphenol present in hygienic and dermatological products. The HCP accumulates preferentially in adipose tissue that is a privileged source of mesenchymal stem cells (MSCs). The evaluation of the potential effects of HCP on MSCs is important for their medical application. Here we examined the effects of HCP on murine adipose tissue-derived stem cells (ADSCs) and human umbilical cord-derived stem cells (UCSCs) in cell culture. We found that 10−4 and 10−5 M HCP inhibits proliferation, osteogenesis and increases apoptosis of ADSCs and UCSCs....
Source: Tissue Engineering and Regenerative Medicine - January 8, 2018 Category: Biotechnology Source Type: research

Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells
In conclusion, USCs are noteworthy cell source in the field of neu ronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - December 28, 2017 Category: Biotechnology Source Type: research

Development of Printable Natural Cartilage Matrix Bioink for 3D Printing of Irregular Tissue Shape
In this study, cartilage acellular matrix (CAM)-based bioink was developed to fabricate functional biomolecule-containing scaffolds. The CAM provides an adequate cartilage tissue –favorable environment for chondrogenic differentiation of cells. Conventional manufacturing techniques such as salt leaching, solvent casting, gas forming, and freeze drying when applied to CAM-based scaffolds cannot precisely control the scaffold geometry for mimicking tissue shape. As an altern ative to the scaffold fabrication methods, 3D printing was recently introduced in the field of tissue engineering. 3D printing may better control ...
Source: Tissue Engineering and Regenerative Medicine - December 28, 2017 Category: Biotechnology Source Type: research

Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
AbstractFast progresses in stem cell-based tooth tissue engineering have been achieved in recent years in several animal models including the mouse, rat, dog, and pig. Moreover, various postnatal mesenchymal stem cells of dental origin have been isolated and shown capable of differentiating into odontoblasts and generating dentin. Meanwhile, human keratinocyte stem/progenitor cells, gingival epithelial cells, and even iPSC-derived epithelium have been demonstrated to be able to differentiate into functional ameloblasts. Translational medicine studies in the nonhuman primate are irreplaceable steps towards clinical applicat...
Source: Tissue Engineering and Regenerative Medicine - December 20, 2017 Category: Biotechnology Source Type: research

The Application of Fibrin/Hyaluronic Acid –Poly( l -Lactic- co -Glycolic Acid) Construct in Augmentation Rhinoplasty
This study evaluated the utility of allogenic chondrocytes with fibrin/hyaluronic acid (HA) –poly(l-lactic-co-glycolic acid) (PLGA) constructs in augmentation rhinoplasty. Chondrocytes from rabbit auricular cartilage were isolated and cultured with fibrin/HA hydrogels and implanted into PLGA scaffolds. After 8  weeks ofin vitro culture, the scaffolds were implanted in the nasal dorsum of six rabbits. Eight weeks postoperatively, the implanted sites were evaluated with gross, radiologic, and histologic analysis.In vitro, more than 90% of the seeded chondrocytes in the PLGA scaffolds survived for 2  weeks, an...
Source: Tissue Engineering and Regenerative Medicine - November 24, 2017 Category: Biotechnology Source Type: research

Chondrogenic Potential of Dedifferentiated Rat Chondrocytes Reevaluated in Two- and Three-Dimensional Culture Conditions
AbstractFor the cartilage repair, the cell sources currently adopted are primarily chondrocytes or mesenchymal stem cells (MSCs). Due to the fact that chondrocytes dedifferentiate during 2-dimensional (2D) expansion, MSCs are generally more studied and considered to have higher potential for cartilage repair purposes. Here we question if the dedifferentiated chondrocytes can regain the chondrogenic potential, to find potential applications in cartilage repair. For this we chose chondrocytes at passage 12 (considered to have sufficiently dedifferentiated) and the expression of chondrogenic phenotypes and matrix syntheses we...
Source: Tissue Engineering and Regenerative Medicine - November 24, 2017 Category: Biotechnology Source Type: research

New Advances in Human X Chromosome Status from a Developmental and Stem Cell Biology
AbstractRecent advances in stem cell biology have dramatically increased the understanding of molecular and cellular mechanism of pluripotency and cell fate determination. Additionally, pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, arose as essential resources for disease modeling and cellular therapeutics. Despite these advancements, the epigenetic dysregulation in pluripotency such as the imprinting status, and X chromosome dosage compensation, and its consequences on future utility of PSCs yet remain unresolved. In this review, we will focus on the X chromosome regulat...
Source: Tissue Engineering and Regenerative Medicine - November 22, 2017 Category: Biotechnology Source Type: research

Endogenous Stem Cells in Homeostasis and Aging
AbstractIn almost all human tissues and organs, adult stem cells or tissue stem cells are present in a unique location, the so-called stem cell niche or its equivalent, continuously replenishing functional differentiated cells. Those endogenous stem cells can be expanded for cell therapeutics usingex vivo cell culture or recalled for tissue repairin situ through cell trafficking and homing. In the aging process, inefficiency in the endogenous stem cell –mediated healing mechanism can emerge from a variety of impairments that accumulate in the processes of stem cell self-renewal, function, differentiation capacity, an...
Source: Tissue Engineering and Regenerative Medicine - November 20, 2017 Category: Biotechnology Source Type: research

Current Understanding of Stem Cell and Secretome Therapies in Liver Diseases
AbstractLiver failure is one of the main risks of death worldwide, and it originates from repetitive injuries and inflammations of liver tissues, which finally leads to the liver cirrhosis or cancer. Currently, liver transplantation is the only effective treatment for the liver diseases although it has a limitation due to donor scarcity. Alternatively, cell therapy to regenerate and reconstruct the damaged liver has been suggested to overcome the current limitation of liver disease cures. Several transplantable cell types could be utilized for recovering liver functions in injured liver, including bone marrow cells, mesenc...
Source: Tissue Engineering and Regenerative Medicine - November 20, 2017 Category: Biotechnology Source Type: research

Recent Advances in Stem Cell Researches and Their Future Perspectives in Regenerative Medicine
(Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - November 17, 2017 Category: Biotechnology Source Type: research

Osteochondral Regeneration with a Scaffold-Free Three-Dimensional Construct of Adipose Tissue-Derived Mesenchymal Stromal Cells in Pigs
AbstractOsteochondral lesion is a major joint disease in humans. Therefore, this study was designed to investigate the regeneration of articular cartilage and subchondral bone, using three-dimensional constructs of autologous adipose tissue-derived mesenchymal stromal cells without any biocompatible scaffolds. Mesenchymal stromal cells were harvested by liposuction from seven pigs, isolated enzymatically, and expanded until construct creation. The pig models had two osteochondral defects (cylindrical defects with a diameter of 5.2  mm and a depth of 5 mm) in one of their patello-femoral grooves. A columnar struct...
Source: Tissue Engineering and Regenerative Medicine - November 15, 2017 Category: Biotechnology Source Type: research

Different Bone Healing Effects of Undifferentiated and Osteogenic Differentiated Mesenchymal Stromal Cell Sheets in Canine Radial Fracture Model
This study was performed to clarify bone healing mechanism of undifferentiated (UCS) and osteogenic (OCS) differentiated mesenchymal stromal cell (MSC) sheets in the fracture model of dogs. UCS and OCS were harvested at 10  days of culture. Transverse fractures at the radius of six beagle dogs were assigned into three groups (n = 4 in each group) i.e. UCS, OCS and control. The fractures were fixed with a 2.7 mm locking plate and six screws. Cell sheets were wrapped around the fracture site. Bones were harvested 8  weeks after operation, then scanned by micro-computed tomography (micro-CT) and analy...
Source: Tissue Engineering and Regenerative Medicine - November 15, 2017 Category: Biotechnology Source Type: research

Extracellular Vesicles in Neurodegenerative Diseases: A Double-Edged Sword
AbstractsExtracellular vesicles (EVs), a heterogenous group of membrane-bound particles, are virtually secreted by all cells and play important roles in cell –cell communication. Loaded with proteins, mRNAs, non-coding RNAs and membrane lipids from their donor cells, these vesicles participate in normal physiological and pathogenic processes. In addition, these sub-cellular vesicles are implicated in the progression of neurodegenerative disorders. Accu mulating evidence suggests that intercellular communication via EVs is responsible for the propagation of key pathogenic proteins involved in the pathogenesis of amyot...
Source: Tissue Engineering and Regenerative Medicine - November 15, 2017 Category: Biotechnology Source Type: research

Synthesis and Biocompatibility Characterizations of in Situ Chondroitin Sulfate –Gelatin Hydrogel for Tissue Engineering
AbstractNovel hydrogel composed of both chondroitin sulfate (CS) and gelatin was developed for better cellular interaction through two step double crosslinking ofN-(3-diethylpropyl)-N-ethylcarbodiimide hydrochloride (EDC) chemistries and then click chemistry. EDC chemistry was proceeded during grafting of amino acid dihydrazide (ADH) to carboxylic groups in CS and gelatin network in separate reactions, thus obtaining CS –ADH and gelatin–ADH, respectively. CS–acrylate and gelatin–TCEP was obtained through a second EDC chemistry of the unreacted free amines of CS–ADH and gelatin–ADH with a...
Source: Tissue Engineering and Regenerative Medicine - November 14, 2017 Category: Biotechnology Source Type: research

Synergistic Effect of Cold Plasma Treatment and RGD Peptide Coating on Cell Proliferation over Titanium Surfaces
AbstractThe aim of this study was to investigate the synergistic effect of cold atmospheric plasma (CAP) treatment and RGD peptide coating for enhancing cellular attachment and proliferation over titanium (Ti) surfaces. The surface structure of CAP-treated and RGD peptide-coated Ti discs were characterized by contact angle goniometer and atomic force microscopy. The effect of such surface modification on human bone marrow derived mesenchymal stem cells (hMSCs) adhesion and proliferation was assessed by cell proliferation and DNA content assays. Besides, hMSCs ’ adhesion and morphology on surface modified Ti discs wer...
Source: Tissue Engineering and Regenerative Medicine - November 10, 2017 Category: Biotechnology Source Type: research

Mesenchymal Stromal Cells from the Maternal Segment of Human Umbilical Cord is Ideal for Bone Regeneration in Allogenic Setting
AbstractUmbilical cord (UC) is a discarded product from the operating theatre and a ready source of mesenchymal stromal cells (MSCs). MSCs from UC express both embryonic and adult mesenchymal stem cell markers and are known to be hypoimmunogenic and non-tumorigenic and thus suitable for allogeneic cell transplantation. Our study aimed to determine the degree of immunotolerance and bone-forming capacity of osteodifferentiated human Wharton ’s jelly-derived mesenchymal stromal cells (hWJ-MSCs) from different segments of UC in an allogenic setting. UCs were obtained from healthy donors delivering a full-term infant by e...
Source: Tissue Engineering and Regenerative Medicine - October 25, 2017 Category: Biotechnology Source Type: research

Implantation of Bone Marrow Stromal Cell Sheets Derived from Old Donors Supports Bone Tissue Formation
AbstractThe purpose of this study was to evaluate the osteogenesis ability of osteogenic matrix cell sheets (OMCS) derived from old donor cells. Bone marrow stromal cells (BMSC) were obtained from young (7-week-old) and old (1-year-old) Fischer344 rats donors and cultured with modified Eagle ’s medium (MEM group) alone or containing dexamethasone (Dex; 10 nM) and ascorbic acid phosphate (AscP; 0.28 mM) (Dex/AscP group). We prepared fourin vitro experimental groups: (1) young MEM, (2) young Dex/AscP, (3) old MEM and (4) old Dex/AscP. Cell proliferation and osteogenic marker mRNA expression levels were evalua...
Source: Tissue Engineering and Regenerative Medicine - October 25, 2017 Category: Biotechnology Source Type: research

Oral Immunization of FMDV Vaccine Using pH-Sensitive and Mucoadhesive Thiolated Cellulose Acetate Phthalate Microparticles
AbstractSeveral barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer ’s patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) prepared by double emulsion technique using cellulose acetate phthalate (CAP) to enhance immune response of foot-and-mouth disease (FMD) virus (FMDV) subun it vaccine. Thiolation of CAP improved mucoadhesive property of CAP to prolong the MPs transit time through the gastrointestinal tract. Thiol...
Source: Tissue Engineering and Regenerative Medicine - October 17, 2017 Category: Biotechnology Source Type: research

Substance-P Ameliorates Dextran Sodium Sulfate-Induced Intestinal Damage by Preserving Tissue Barrier Function
In this study, we explored the therapeutic effect of SP on dextran sodium sulfate-induced intestine damage by injecting SP. The effects of SP were evaluated by analyzing crypt structures, proliferating cell pool and infiltration of immune cells. DSS treatment provoked abnormal immune response and disruption of intestine epithelial barrier. However, co-treatment of SP obviously blocked the development of intestinal damages by declining inflammatory responses and sustaining intestinal structure more intact. The treatment of SP to chronic damages also promoted intestinal regeneration by preserving the integrity of colon tissu...
Source: Tissue Engineering and Regenerative Medicine - October 13, 2017 Category: Biotechnology Source Type: research

Non-contact Coculture Reveals a Comprehensive Response of Chondrocytes Induced by Mesenchymal Stem Cells Through Trophic Secretion
AbstractCoculture between mesenchymal stem cells (MSCs) and chondrocytes has significant implications in cartilage regeneration. However, a conclusive understanding remains elusive. Previously, we reported that rabbit bone marrow-derived MSCs (rbBMSCs) could downregulate the differentiated phenotype of rabbit articular chondrocytes (rbACs) in a non-contact coculture system for the first time. In the present study, a systemic investigation was performed to understand the biological characteristics of chondrocytes in coculture with MSCs. Firstly, cells (MSCs and chondrocytes) from different origins were cocultured in transwe...
Source: Tissue Engineering and Regenerative Medicine - September 27, 2017 Category: Biotechnology Source Type: research

Potency of Human Urine-Derived Stem Cells for Renal Lineage Differentiation
In this study, the ability of USCs to differentiate into renal lineage cells was compared with that of adipose tissue-derived stem cells (ADSCs) and amniotic fluid-derived stem cells (AFSCs), with respect to surf ace antigen expression, morphology, immunocytochemistry, renal lineage gene expression, secreted factors, immunomodulatory marker expression,in vivo safety, and renal differentiation potency. Undifferentiated USCs were positive for CD44 and CD73, negative for CD34 and CD45, and formed aggregates after 3  weeks of renal differentiation. Undifferentiated USCs showed high SSEA4 expression, while renal-differenti...
Source: Tissue Engineering and Regenerative Medicine - September 27, 2017 Category: Biotechnology Source Type: research

Calcium Microcrystal Formation in Recurrent Herniation Patients After Autologous Disc Cell Transplantation
This study aims to investigate the presence of structural phenomena accompanying the transformation process after ADCT treatment in IVD disease. Structural phenomena of ADCT-treated patients (Group 1, n  = 10) with recurrent disc herniation were compared to conventionally-treated patients with recurrent herniation (Group 2, n = 10) and patients with a first-time herniation (Group 3, n = 10). For ethical reasons, a control group of ADCT patients who did not have a recurrent disc herniation was not possible. Tissue samples were obtained via micro-sequestrectomy after disc herniation and analyzed...
Source: Tissue Engineering and Regenerative Medicine - September 22, 2017 Category: Biotechnology Source Type: research

Adult Stem Cells of Orofacial Origin: Current Knowledge and Limitation and Future Trend in Regenerative Medicine
AbstractStem cell research is one of the most rapidly expanding field of medicine which provides significant opportunities for therapeutic and regenerative applications. Different types of stem cells have been isolated investigating their accessibility, control of the differentiation pathway and additional immunomodulatory properties. Bulk of the literature focus has been on the study and potential applications of adult stem cells (ASC) because of their low immunogenicity and reduced ethical considerations. This review paper summarizes the basic available literature on different types of ASC with special focus on stem cell...
Source: Tissue Engineering and Regenerative Medicine - September 22, 2017 Category: Biotechnology Source Type: research

Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages
AbstractMSC-based therapy is providing a cure for degenerative diseases with unmet medical need and usually iliac crest bone marrow (ICBM) are being applied in clinics. Alternative sources, including adipose tissue and reamer/irrigator/aspirator hold great potential for isolating MCSs. Here, we compared original MSCs features of adipose tissue (Ad-MSCs) and bone marrow of long-bone (RIA-MSCs) or iliac crest, and the expression of chemokine receptors (includingCXCR4,CX3CR1,CXCR6,CXCR2,CCR1 andCCR7) in these three sources, which are important in the context of homing. We further investigated the role of SDF-1/CXCR4 axis as a...
Source: Tissue Engineering and Regenerative Medicine - September 19, 2017 Category: Biotechnology Source Type: research

Atelocollagen-based Hydrogels Crosslinked with Oxidised Polysaccharides as Cell Encapsulation Matrix for Engineered Bioactive Stromal Tissue
In this study, a bioactive stroma was obtained considering two main components: 1) the artificial ECM formed using atelocollagen-oxidized polysaccharides hydrogels in which the polysaccharide compound (oxidised gellan or pullulan) has the role of crosslinker and 2) encapsulated stromal cells (dermal fibroblasts, ovarian theca-interstitial and granulosa cells). The cell-hosting ability of the hydrogels is demonstrated by a good diffusion of globular proteins (albumin) while the fibrillar morphology proves to be optimal for cell adhesion. These structural properties and cytocompatibility of the components maintain good cell ...
Source: Tissue Engineering and Regenerative Medicine - September 19, 2017 Category: Biotechnology Source Type: research

Novel Approach Toward the Generation of Tissue Engineered Heart Valve by Using Combination of Antioxidant and Detergent: A Potential Therapy in Cardiovascular Tissue Engineering
AbstractTo develop decellularized heart valve scaffold from porcine for heart valve regeneration. Porcine heart valves were decellularized with unique optimized approach by using 1% sodium dodecyl sulfate solution and 5% dimethyl sulfoxide for the first time. Effect of decellularization process on scaffold were characterized by hematoxylin –eosin, 4′,6-diamidino-2-phenylindole, Masson’s trichrome, alcian blue staining and scanning electron microscopy for extracellular matrix (ECM) analysis in scaffold. The results showed that developed protocol for decellularization of heart valve scaffold shown complete ...
Source: Tissue Engineering and Regenerative Medicine - September 18, 2017 Category: Biotechnology Source Type: research

Serial Analysis of Tracheal Restenosis After 3D-Printed Scaffold Implantation: Recruited Inflammatory Cells and Associated Tissue Changes
AbstractTracheal restenosis is a major obstacle to successful tracheal replacement, and remains the greatest challenge in tracheal regeneration. However, there have been no detailed investigations of restenosis. The present study was performed to analyze the serial changes in recruited inflammatory cells and associated histological changes after tracheal scaffold implantation. Asymmetrically porous scaffolds, which successfully prevented tracheal stenosis in a partial trachea defect model, designed with a tubular shape by electrospinning and reinforced by 3D-printing to reconstruct 2-cm circumferential tracheal defect. Ser...
Source: Tissue Engineering and Regenerative Medicine - September 13, 2017 Category: Biotechnology Source Type: research