Correction to: The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages
Unfortunately, the online published article has error in Figure  3. The name of protein, “Gelectin-1” should be corrected to “Galectin-3”. The corrected Fig. 3 is placed in the following page. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 12, 2019 Category: Biotechnology Source Type: research

Genome Engineering for Osteoarthritis: From Designer Cells to Disease-Modifying Drugs
CONCLUSION:With recent advances in the development of genome editing technologies such as the CRISPR-Cas9 system, these genetic and epigenetic alternations in OA can be used as platforms from which potential biomarkers for the diagnosis, prognosis, drug response, and development of potential personalized therapeutic targets for OA can be approached. Furthermore, genome editing has allowed the development of “designer” cells, whereby the receptors, gene regulatory networks, or transgenes can be modified as a basis for new cell-based therapies. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 5, 2019 Category: Biotechnology Source Type: research

D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation
CONCLUSION:This study demonstrates that our nHA/PA66/D-RADA16-RGD composite exhibits reasonable mechanical properties, biocompatibility and bioactivity with promotion of bone formation. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 5, 2019 Category: Biotechnology Source Type: research

Titanium Powder Coating Using Metal 3D Printing: A Novel Coating Technology for Cobalt –Chromium Alloy Implants
AbstractBackground:Three-dimensional (3D) printing with a direct metal fabrication (DMF) technology has been innovatively introduced in the field of surface treatment of prostheses. The purpose of this study was to determine whether such modifications on the surface of cobalt –chromium (CoCr) alloy by titanium powder coating using DMF improves the osseointegration ability of CoCr alloy.Methods:We compared the in vitro and in vivo ability of cells to adhere to DMF-coated CoCr alloy with machining. Biological and morphological responses to human osteoblast cell lines were examined by measuring cell proliferation rate a...
Source: Tissue Engineering and Regenerative Medicine - January 4, 2019 Category: Biotechnology Source Type: research

A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices
AbstractBackground:Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits.Methods:The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxyli...
Source: Tissue Engineering and Regenerative Medicine - December 8, 2018 Category: Biotechnology Source Type: research

Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin Model
Conclusion:These results suggested that hE-MSCs have a potency of immune modulation in psoriasis, which might be the key factor for the improved psoriasis. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - December 1, 2018 Category: Biotechnology Source Type: research

Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues
Conclusion:This hybrid biofabrication process can be established as a highly efficient and scalable platform to fabricate biomimetic scaffolds with patterned fibrous microstructure, and will facilitate future development of clinical solutions for musculoskeletal tissue regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - December 1, 2018 Category: Biotechnology Source Type: research

Induction of Chondrogenic Differentiation in Human Mesenchymal Stem Cells Cultured on Human Demineralized Bone Matrix Scaffold under Hydrostatic Pressure
In this study, CHP was applied to human bone marrow mesenchymal stem cells (hBMSCs) seeded on DBM scaffolds at a pressure of 5  MPa with a frequency of 0.5 Hz and 4 h per day for 1 week. Changes in chondrogenic and osteogenic gene expressions were analyzed by quantifying mRNA signal level of Sox9, collagen type I, collagen type II, aggrecan (ACAN), Osteocalcin, and Runx2. Histological analysis was carried out by hematox ylin and eosin, and Alcian blue staining. Moreover, DMMB and immunofluorescence staining were used for glycosaminoglycan (GAG) and collagen type II detection, respectively.Results:Real-t...
Source: Tissue Engineering and Regenerative Medicine - November 17, 2018 Category: Biotechnology Source Type: research

Combined Treatment with Low-Level Laser and rhBMP-2 Promotes Differentiation and Mineralization of Osteoblastic Cells under Hypoxic Stress
Conclusion:Combined treatment with rhBMP-2 and LLL induced differentiation and mineralization of hypoxic-cultured MC3T3-E1 osteoblasts by activating p38/PKD signalingin vitro. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - November 17, 2018 Category: Biotechnology Source Type: research

GM-CSF Enhances Mobilization of Bone Marrow Mesenchymal Stem Cells via a CXCR4-Medicated Mechanism
Conclusion:These results suggest that GM-CSF is a useful tool for mobilizing BM MSCs into the PB. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - November 15, 2018 Category: Biotechnology Source Type: research

Local Silencing of Connective Tissue Growth Factor by siRNA/Peptide Improves Dermal Collagen Arrangements
Conclusion:CTGF expression was down-regulated to 40% of control by CTGF siRNA/KALA (1:24) complexes (p 
Source: Tissue Engineering and Regenerative Medicine - November 14, 2018 Category: Biotechnology Source Type: research

Conditioned Medium from Tonsil-Derived Mesenchymal Stem Cells Relieves CCl 4 -Induced Liver Fibrosis in Mice
Conclusions:Taken together, these data support the therapeutic potential of T-MSC CM and/or IL-1Ra for the alleviation of liver fibrosis, as well as in treating diseases involving organ fibrosis. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 19, 2018 Category: Biotechnology Source Type: research

In Vivo Safety and Regeneration of Long-Term Transported Amniotic Fluid Stem Cells for Renal Regeneration
Conclusion:This study demonstrates that long-term transported cells under optimized conditions can be used for cell therapy without adverse effects on stem cell characteristics,in vivo safety, and tissue regeneration potency. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 11, 2018 Category: Biotechnology Source Type: research

Biopolymeric In Situ Hydrogels for Tissue Engineering and Bioimaging Applications
Conclusion:The applications of bioactive injectable hydrogels and their bioimaging are a promising area in tissue engineering and regenerative medicine. There is a high demand for injectable hydrogels forin situ imaging. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Hydroxyapatite Nanorod-Modified Sand Blasted Titanium Disk for Endosseous Dental Implant Applications
Conclusions:The modification of Ti implants byl-glutamic acid grafted nHA has produced highly osteogenic Ti disk plants in comparison to pristine Ti disk implants due to the formation of bioactive surfaces by hydroxyapatite nano rods on Ti disk implants. Ti-nHA disk implants showed enhanced adhesion, proliferation, and MC3T3-E1 cells viability in comparison to pristine Ti disk implants. Thus nHA might be to be useful to enhance the osseointegration of Ti implants with teeth bones. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Combination of Mesenchymal Stem Cells, Cartilage Pellet and Bioscaffold Supported Cartilage Regeneration of a Full Thickness Articular Surface Defect in Rabbits
CONCLUSION:HyalofastTM together with BM-MSCs and CP led to efficient cartilage regeneration following full thickness surgical ablation of tibial articular cartilagein vivo in rabbits. Presence of hyaluronic acid in the scaffold and native microenvironment cues probably facilitated differentiation and integration of BM-MSCs. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts
Conclusion:FN-immobilized microgrooved Ti can act as an effective surface for enhancing fibroblast proliferation, and can be used for promoting soft tissue response on the connective tissue attachment zone of biomaterial surfaces. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Injectable Biomaterials in Plastic and Reconstructive Surgery: A Review of the Current Status
Conclusions:The usage of injectable biomaterials has been increasing for the tissue restoration and regeneration. The future of incorporating ADSCs and growth factors into the injectable biomaterials is promising. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine
Conclusion:In this review, we discuss the use of in situ cross-linkable hydrogels in tissue engineering and regenerative medicine applications. In particular, we focus on emerging technologies as a powerful therapeutic tool for tissue regenerative medicine applications. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Polypeptide Thermogels as Three-Dimensional Scaffolds for Cells
Conclusion:In addition to all the advantages of thermogels including mild conditions for cell-incorporation and controlled supplies of the growth factors, polypeptide thermogels provide neutral pH environments to the cells during the degradation of the gel. Polypeptide thermogels as an injectable scaffold can be a promising system for their eventualin vivo applications in stem cell therapy. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications
Conclusion:Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications inin situ 3D bioprinting for tissue or organ regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Full Thickness Skin Expansion ex vivo in a Newly Developed Reactor and Evaluation of Auto-Grafting Efficiency of the Expanded Skin Using Yucatan Pig Model
Conclusions:These findings provided insight on the potential of this expansion system in promoting dermal extracellular matrix synthesisin vitro. Conclusively, this newly developed smart skin bioreactor enabled effective skin expansionex vivo and successful graftingin vivo in a pig model. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2
Conclusion:PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
CONCLUSION:Our findings can provide a foundation to optimization of conditions for stem cell transport. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Electrostatically Interactive Injectable Hydrogels for Drug Delivery
Conclusion:The review concludes with perspectives on the future of electrostatically interactivein situ-forming hydrogels. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Characterization of Human Fetal Cartilage Progenitor Cells During Long-Term Expansion in a Xeno-Free Medium
CONCLUSION:These results suggest that SFM-XF supports the long-term expansion of hFCPCs without significant phenotypic and chromosomal changes. This study have also shown that hFCPCs can be mass-producedin vitro, proving their commercial value as a novel source for developing cell therapies. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Injectable Hydrogels for Regenerative Medicine
(Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - October 1, 2018 Category: Biotechnology Source Type: research

Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration
Conclusion:We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 21, 2018 Category: Biotechnology Source Type: research

Biopolymeric In Situ Hydrogels for Tissue Engineering and Bioimaging Applications
Conclusion:The applications of bioactive injectable hydrogels and their bioimaging are a promising area in tissue engineering and regenerative medicine. There is a high demand for injectable hydrogels forin situ imaging. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 14, 2018 Category: Biotechnology Source Type: research

Injectable Hydrogels for Regenerative Medicine
(Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 11, 2018 Category: Biotechnology Source Type: research

Late Complications of Allogenic Stem Cells Transplantation in Leukaemia
Conclusion:While allogenic stem cell transplantation is an effective cure for leukaemia, the procedure is associated with complications that can have their onset many years after the procedure. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 6, 2018 Category: Biotechnology Source Type: research

Injectable Biomaterials in Plastic and Reconstructive Surgery: A Review of the Current Status
Conclusions:The usage of injectable biomaterials has been increasing for the tissue restoration and regeneration. The future of incorporating ADSCs and growth factors into the injectable biomaterials is promising. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 1, 2018 Category: Biotechnology Source Type: research

Full Thickness Skin Expansion ex vivo in a Newly Developed Reactor and Evaluation of Auto-Grafting Efficiency of the Expanded Skin Using Yucatan Pig Model
Conclusions:These findings provided insight on the potential of this expansion system in promoting dermal extracellular matrix synthesisin vitro. Conclusively, this newly developed smart skin bioreactor enabled effective skin expansionex vivo and successful graftingin vivo in a pig model. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - September 1, 2018 Category: Biotechnology Source Type: research

Glucosamine Hydrochloride and N -Acetylglucosamine Influence the Response of Bovine Chondrocytes to TGF- β3 and IGF in Monolayer and Three-Dimensional Tissue Culture
Conclusion:GlcN ·HCl and GlcNAc increased cell growth and stimulated COL2 synthesis in long-time 3D culture. However, only GlcNAc added to GF improved proteoglycan synthesis. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 30, 2018 Category: Biotechnology Source Type: research

The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages
CONCLUSION:Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 28, 2018 Category: Biotechnology Source Type: research

Proliferation Profile of Uterine Endometrial Stromal Cells during In Vitro Culture with Gonadotropins: Recombinant versus Urinary Follicle Stimulating Hormone
Conclusions:FSH may inhibit the proliferation of murine uterine endometrial stromal cells duringin vitro culture. rFSH may have more significant inhibitory effects on the proliferation of endometrial stromal cells than uFSH. Establishing an optimal endocrine milieu is necessary using more advanced combination of female hormones forin vitro culture of this type of cells. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 28, 2018 Category: Biotechnology Source Type: research

The Effect of Fibronectin-Immobilized Microgrooved Titanium Substrata on Cell Proliferation and Expression of Genes and Proteins in Human Gingival Fibroblasts
Conclusion:FN-immobilized microgrooved Ti can act as an effective surface for enhancing fibroblast proliferation, and can be used for promoting soft tissue response on the connective tissue attachment zone of biomaterial surfaces. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 28, 2018 Category: Biotechnology Source Type: research

The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages
CONCLUSION:Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 28, 2018 Category: Biotechnology Source Type: research

Proliferation Profile of Uterine Endometrial Stromal Cells during In Vitro Culture with Gonadotropins: Recombinant versus Urinary Follicle Stimulating Hormone
Conclusions:FSH may inhibit the proliferation of murine uterine endometrial stromal cells duringin vitro culture. rFSH may have more significant inhibitory effects on the proliferation of endometrial stromal cells than uFSH. Establishing an optimal endocrine milieu is necessary using more advanced combination of female hormones forin vitro culture of this type of cells. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 28, 2018 Category: Biotechnology Source Type: research

In Situ Cross-Linkable Hydrogels as a Dynamic Matrix for Tissue Regenerative Medicine
Conclusion:In this review, we discuss the use of in situ cross-linkable hydrogels in tissue engineering and regenerative medicine applications. In particular, we focus on emerging technologies as a powerful therapeutic tool for tissue regenerative medicine applications. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 27, 2018 Category: Biotechnology Source Type: research

Hydroxyapatite Nanorod-Modified Sand Blasted Titanium Disk for Endosseous Dental Implant Applications
Conclusions:The modification of Ti implants byl-glutamic acid grafted nHA has produced highly osteogenic Ti disk plants in comparison to pristine Ti disk implants due to the formation of bioactive surfaces by hydroxyapatite nano rods on Ti disk implants. Ti-nHA disk implants showed enhanced adhesion, proliferation, and MC3T3-E1 cells viability in comparison to pristine Ti disk implants. Thus nHA might be to be useful to enhance the osseointegration of Ti implants with teeth bones. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 25, 2018 Category: Biotechnology Source Type: research

Efficient Production of Murine Uterine Damage Model
Conclusion:Taken together, murine uterine damage models were most successfully established by chemical treatment. This production protocols could be applied further to larger animals such as non-human primate. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 18, 2018 Category: Biotechnology Source Type: research

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications
Conclusion:Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications inin situ 3D bioprinting for tissue or organ regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 16, 2018 Category: Biotechnology Source Type: research

Keratinocyte Migration in a Three-Dimensional In Vitro Wound Healing Model Co-Cultured with Fibroblasts
Conclusion:This 3Din vitro wound healing model is a step towards developing a mimetic construct that recapitulates the complex microenvironment of healing wounds and could aid in the early studies of novel therapeutics that promote migration and proliferation of epithelial cells. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly( ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering
Conclusion:Compared to other groups, PCEC-CS nanofibers incorporated with 15  wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research

Polypeptide Thermogels as Three-Dimensional Scaffolds for Cells
Conclusion:In addition to all the advantages of thermogels including mild conditions for cell-incorporation and controlled supplies of the growth factors, polypeptide thermogels provide neutral pH environments to the cells during the degradation of the gel. Polypeptide thermogels as an injectable scaffold can be a promising system for their eventualin vivo applications in stem cell therapy. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research