Efficient Production of Murine Uterine Damage Model
Conclusion:Taken together, murine uterine damage models were most successfully established by chemical treatment. This production protocols could be applied further to larger animals such as non-human primate. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 18, 2018 Category: Biotechnology Source Type: research

Click Chemistry-Based Injectable Hydrogels and Bioprinting Inks for Tissue Engineering Applications
Conclusion:Interestingly, the emergence of click chemistry reactions in bioink synthesis for 3D bioprinting have shown the massive potential of these reaction methods in creating 3D tissue constructs. However, the limitations and challenges involved in the click chemistry reactions should be analyzed and bettered to be applied to tissue engineering and 3D bioinks. The future scope of these materials is promising, including their applications inin situ 3D bioprinting for tissue or organ regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 16, 2018 Category: Biotechnology Source Type: research

Keratinocyte Migration in a Three-Dimensional In Vitro Wound Healing Model Co-Cultured with Fibroblasts
Conclusion:This 3Din vitro wound healing model is a step towards developing a mimetic construct that recapitulates the complex microenvironment of healing wounds and could aid in the early studies of novel therapeutics that promote migration and proliferation of epithelial cells. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research

A Comparison of the Effects of Silica and Hydroxyapatite Nanoparticles on Poly( ε-caprolactone)-Poly(ethylene glycol)-Poly(ε-caprolactone)/Chitosan Nanofibrous Scaffolds for Bone Tissue Engineering
Conclusion:Compared to other groups, PCEC-CS nanofibers incorporated with 15  wt% n-HA were able to support more cell adhesion and differentiation, thus are better candidates for bone tissue engineering applications. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research

Polypeptide Thermogels as Three-Dimensional Scaffolds for Cells
Conclusion:In addition to all the advantages of thermogels including mild conditions for cell-incorporation and controlled supplies of the growth factors, polypeptide thermogels provide neutral pH environments to the cells during the degradation of the gel. Polypeptide thermogels as an injectable scaffold can be a promising system for their eventualin vivo applications in stem cell therapy. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 14, 2018 Category: Biotechnology Source Type: research

Electrostatically Interactive Injectable Hydrogels for Drug Delivery
Conclusion:The review concludes with perspectives on the future of electrostatically interactivein situ-forming hydrogels. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 9, 2018 Category: Biotechnology Source Type: research

Current Status of Stem Cell Treatment for Type I Diabetes Mellitus
CONCLUSION:Stem cell transplantation may prove to be a safe and effective treatment for patients with Type 1 diabetes mellitus. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - August 2, 2018 Category: Biotechnology Source Type: research

Collagen Scaffolds in Cartilage Tissue Engineering and Relevant Approaches for Future Development
Conclusion:Considering the previous improvements of physical and compositional properties of collagen scaffolds and recent manufacturing developments of structural polymer, it is concluded that hybrid strategy is a promising approach to advance further collagen-based scaffolds in CTE. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 25, 2018 Category: Biotechnology Source Type: research

Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity
Conclusion:Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 24, 2018 Category: Biotechnology Source Type: research

A 4-Axis Technique for Three-Dimensional Printing of an Artificial Trachea
Conclusion:We suggest that the 4-axis FDM process is more suitable for the development of an accurate and mechanically superior trachea scaffold. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 14, 2018 Category: Biotechnology Source Type: research

Combination of Mesenchymal Stem Cells, Cartilage Pellet and Bioscaffold Supported Cartilage Regeneration of a Full Thickness Articular Surface Defect in Rabbits
CONCLUSION:HyalofastTM together with BM-MSCs and CP led to efficient cartilage regeneration following full thickness surgical ablation of tibial articular cartilagein vivo in rabbits. Presence of hyaluronic acid in the scaffold and native microenvironment cues probably facilitated differentiation and integration of BM-MSCs. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 13, 2018 Category: Biotechnology Source Type: research

Cellular Response of Anodized Titanium Surface by Poly(Lactide-co-Glycolide)/Bone Morphogenic Protein-2
Conclusion:PLG polymers mixed with rhBMP-2 might improve proliferation, differentiation and osteogenic protein formation of cells on the anodized titanium. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 13, 2018 Category: Biotechnology Source Type: research

Wicking Property of Graft Material Enhanced Bone Regeneration in the Ovariectomized Rat Model
AbstractBackground:Recruitment and homing cells into graft materials from host tissue is crucial for bone regeneration.Methods:Highly porous, multi-level structural, hydroxyapatite bone void filler (HA-BVF) have been investigated to restore critical size bone defects. The aim was to investigate a feasibility of bone regeneration of synthetic HA-BVF compared to commercial xenograft (Bio-Oss). HA-BVF of 0.7  mm in average diameter was prepared via template coating method. Groups of animals (n = 6) were divided into two with normal (Sham) or induced osteoporotic conditions (Ovx). Subsequently, subdivided in...
Source: Tissue Engineering and Regenerative Medicine - July 13, 2018 Category: Biotechnology Source Type: research

Three-Dimensional Spheroid Culture Increases Exosome Secretion from Mesenchymal Stem Cells
Conclusion:These results suggested that 3D spheroid culture produces more exosomes than 2D culture and the non-adherent round cell morphology itself might be a causative factor. The result of the present study could provide useful information to develop an optimal process for the mass production of exosomes. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 12, 2018 Category: Biotechnology Source Type: research

Characterization of Human Fetal Cartilage Progenitor Cells During Long-Term Expansion in a Xeno-Free Medium
CONCLUSION:These results suggest that SFM-XF supports the long-term expansion of hFCPCs without significant phenotypic and chromosomal changes. This study have also shown that hFCPCs can be mass-producedin vitro, proving their commercial value as a novel source for developing cell therapies. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 10, 2018 Category: Biotechnology Source Type: research

Fabrication of Microchannels and Evaluation of Guided Vascularization in Biomimetic Hydrogels
CONCLUSION:The strategy of fabricating microchannels in a biomimetic hydrogel and simultaneously providing a chemical cue for angiogenesis is a promising formula for large-scale tissue regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 10, 2018 Category: Biotechnology Source Type: research

Mesenchymal Stem Cells of Different Origin-Seeded Bioceramic Construct in Regeneration of Bone Defect in Rabbit
CONCLUSION:In vivo experiments in rabbit CSD model confirmed that autogenous, allogenous and xenogenous BMSC seeded on bioscaffold promoted faster healing of critical size defects. Hence, we may suggest that BMSCs are suitable for bone formation in fracture healing and non-union. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 10, 2018 Category: Biotechnology Source Type: research

Comparison of Angiogenic Activities of Three Neuropeptides, Substance P, Secretoneurin, and Neuropeptide Y Using Myocardial Infarction
CONCLUSION:In our comparison study, the SP-immobilized hydrogel shows the highest angiogenic activity and tissue regeneration among the test groups. This result suggests that nerve regeneration factors help angiogenesis in damaged tissues, which also highlights the importance of the neuro-angiogenic peptides as an element of tissue regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 5, 2018 Category: Biotechnology Source Type: research

Optimal Stem Cell Transporting Conditions to Maintain Cell Viability and Characteristics
CONCLUSION:Our findings can provide a foundation to optimization of conditions for stem cell transport. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - July 3, 2018 Category: Biotechnology Source Type: research

In Vivo Validation Model of a Novel Anti-Inflammatory Scaffold in Interleukin-10 Knockout Mouse
CONCLUSION:These results indicate that the MH-PLGA scaffold had anti-inflammatory effects and high renal regeneration potency. Therefore, IL-10 KO mice are a suitable animal model forin vivo validation of novel anti-inflammatory scaffolds. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 23, 2018 Category: Biotechnology Source Type: research

The Use of Normal Stem Cells and Cancer Stem Cells for Potential Anti-Cancer Therapeutic Strategy
CONCLUSION:There has been a continuing increase in the number of studies on therapeutic stem cells and CSC-specific markers for selective diagnosis and therapy of cancer. This review focuses on the current status in the use of normal stem cells and CSCs for targeted cancer therapy. Future direction is also proposed. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 21, 2018 Category: Biotechnology Source Type: research

Positive Effects of Bisphosphonates on Osteogenic Differentiation in Patient-Derived Mesenchymal Stem Cells for the Treatment of Osteoporosis
CONCLUSION:Our study using a clinically relevantin vitro model suggests that bisphosphonate treatment is more effective for patients with osteoporosis than its preventive effect for healthy person. In addition, patient-specific responses to bisphosphonates should be considered rather than bisphosphonate type prior to prescription. Further investigations are needed to determine how bisphosphonates influence hBMSCs function to mediate bone quality and turnover in osteoporotic patients. Such studies can generate novel approaches to treat age-related osteoporotic bone loss. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 8, 2018 Category: Biotechnology Source Type: research

Thermoresponsive and Biodegradable Amphiphilic Block Copolymers with Pendant Functional Groups
CONCLUSION:MCL-(CO), MCL-(COOH), and MCL-(NH2) with ketone, COOH, and NH2 pendant groups showed solution-to-hydrogel phase transitions and biodegradation behaviors that depended on both the type and number of pendant groups. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 4, 2018 Category: Biotechnology Source Type: research

A Novel Dorsal Slit Approached Non-Ischemic Partial Nephrectomy Method for a Renal Tissue Regeneration in a Mouse Model
CONCLUSION:The Non-IR and dorsal slit method for kidney surgery in a mouse model can be an alternative surgical approach for researchers without adverse reactions such as apoptosis, inflammation, fibrosis, functional impairment, and systemic reactions. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - June 1, 2018 Category: Biotechnology Source Type: research

In Vitro and In Vivo Osteogenesis of Human Orbicularis Oculi Muscle-Derived Stem Cells
CONCLUSION:Our data suggest that human OOM tissue is a valuable and noninvasive resource for osteoprogenitor cells to be used in bone repair and regeneration. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 29, 2018 Category: Biotechnology Source Type: research

A Pathophysiological Validation of Collagenase II-Induced Biochemical Osteoarthritis Animal Model in Rabbit
CONCLUSION:These study shows that biochemically induced cartilage degeneration truly represented uniform and reliable OA state. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 29, 2018 Category: Biotechnology Source Type: research

Correction to: Endogenous Stem Cells in Homeostasis and Aging
Regrettably, in the previous version of this article, two important references ([47-2, 173] were missing. The authors would like to make following corrections in the original version of the article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - May 19, 2018 Category: Biotechnology Source Type: research

MiR-214 Regulates the Human Hair Follicle Stem Cell Proliferation and Differentiation by Targeting EZH2 and Wnt/ β-Catenin Signaling Way In Vitro
AbstractmiR-214 plays a major role in the self-renewal of skin tissue. However, whether miR-214 regulates the proliferation and differentiation of human hair follicle stem cells (HFSCs) is unknown. Primary HFSCs were isolated from human scalp skin tissue, cultured, and identified using flow cytometry. An miR-214 mimic and inhibitor were constructed for transfection into HFSCs. The MTS and colony formation assays examined cell proliferation. Immunofluorescence detected the localization and expression levels of TCF4, β-catenin, and differentiation markers. Luciferase reporter and TOP/FOP Flash assays investigated whethe...
Source: Tissue Engineering and Regenerative Medicine - May 7, 2018 Category: Biotechnology Source Type: research

In Vitro Anti-Inflammation and Chondrogenic Differentiation Effects of Inclusion Nanocomplexes of Hyaluronic Acid-Beta Cyclodextrin and Simvastatin
AbstractThe aim of this study was to prepare inclusion nanocomplexes of hyaluronic acid- β-cyclodextrin and simvastatin (HA-β-CD/SIM) and evaluatein vitro anti-inflammation effects on lipopolysaccharide (LPS)-activated synoviocytes and chondrogenic differentiation effects on rat adipose-derived stem cells (rADSCs). The β-CD moieties in HA-β-CD could incorporate SIM to form HA-β-CD/SIM nanocomplexes with diameters of 297–350 nm. HA-β-CD/SIM resulted in long-term release of SIM from the nanocomplexes for up to 63 days in a sustained manner.In vitro studies revealed that HA- &beta...
Source: Tissue Engineering and Regenerative Medicine - May 3, 2018 Category: Biotechnology Source Type: research

Nondestructive Assessment of Glycosaminoglycans in Engineered Cartilages Using Hexabrix-Enhanced Micro-Computed Tomography
This study evaluated the feasibility of using micro-computed tomography ( µCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffoldsin vitro. Engineered cartilages were incubated with Hexabrix 320 for 20  min and analyzed via µCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D µCT images of...
Source: Tissue Engineering and Regenerative Medicine - April 25, 2018 Category: Biotechnology Source Type: research

Remifentanil Negatively Regulates RANKL-Induced Osteoclast Differentiation and Bone Resorption by Inhibiting c-Fos/NFATc1 Expression
This study shows that remifentanil inhibits the differentiation and maturation of osteoclasts and reduces bone resorption. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - April 21, 2018 Category: Biotechnology Source Type: research

Schwann Cells Enhance Penetration of Regenerated Axons into Three-Dimensional Microchannels
AbstractNerve regeneration after injury requires proper axon alignment to bridge the lesion site and myelination to achieve functional recovery. Transplanted scaffolds with aligned channels, have been shown to induce axon growth to some extent. However, the penetration of axons into the microchannels remain a challenge, influencing the functional recovery of regenerated nerves. We previously demonstrated that the size of microchannels exerts significant impact on Schwann cells (SCs) migration. Here we demonstrate that migration of SCs promotes, significantly, the dorsal root ganglion (DRG) neurons to extend axons into thre...
Source: Tissue Engineering and Regenerative Medicine - February 28, 2018 Category: Biotechnology Source Type: research

In Vivo Biocompatibility and Improved Compression Strength of Reinforced Keratin/Hydroxyapatite Scaffold
AbstractA rapid freezing/lyophilizing/reinforcing process is suggested to fabricate reinforced keratin/hydroxyapatite (HA) scaffold with improved mechanical property and biocompatibility for tissue engineering. The keratin, extracted from human hair, and HA mixture were rapidly frozen with liquid nitrogen and then lyophilized to prepare keratin/HA laminar scaffold. The scaffold was then immersed in PBS for reinforcement treatment, and followed by a second lyophilization to prepare the reinforced keratin/HA scaffold. The morphology, mechanical, chemical, crystal and thermal property of the keratin/HA scaffold were investiga...
Source: Tissue Engineering and Regenerative Medicine - February 23, 2018 Category: Biotechnology Source Type: research

A Non-woven Path: Electrospun Poly(lactic acid) Scaffolds for Kidney Tissue Engineering
AbstractChronic kidney disease is a major global health problem affecting millions of people; kidney tissue engineering provides an opportunity to better understand this disease, and has the capacity to provide a cure. Two-dimensional cell culture and decellularised tissue have been the main focus of this research thus far, but despite promising results these methods are not without their shortcomings. Polymer fabrication techniques such as electrospinning have the potential to provide a non-woven path for kidney tissue engineering. In this experiment we isolated rat primary kidney cells which were seeded on electrospun po...
Source: Tissue Engineering and Regenerative Medicine - February 14, 2018 Category: Biotechnology Source Type: research

Mechanically Reinforced Extracellular Matrix Scaffold for Application of Cartilage Tissue Engineering
In this study, decellularized porcine cartilage-derived extracellular matrix (ECM) was utilized to fabricate ECM scaffolds. Mechanically reinforced ECM scaffolds were developed by combining salt-leaching and crosslinking for cartilage repair. The developed scaffolds were investigated with respect to their physicochemical properties and their cartilage tissue formation ability. The mechanically reinforced ECM scaffold showed similar mechanical strength to that of synthetic PLGA scaffold and expressed higher levels of cartilage-specific markers compared to those expressed by the ECM scaffold prepared by simple freeze-drying....
Source: Tissue Engineering and Regenerative Medicine - February 8, 2018 Category: Biotechnology Source Type: research

In Vivo Observation of Endothelial Cell-Assisted Vascularization in Pancreatic Cancer Xenograft Engineering
In this study, for better understanding of patient-derived xenograft (PDX) generation, angiogenic characteristics during PDX cancerous tissue generation was investigated with different initial cell seeding conditions in the hydrogel. We monitored the angiogenic changes during the formation ofin vivo cancer cell line xenografts induced by endothelial cells. Ourin vivo cancer tissue formation system was designed with the assistance of tissue engineering technology to mimic patient-derived xenograft formation. Endothelial cells and MIA PaCa-2 pancreatic carcinoma cells were encapsulated in fibrin gel at different mixing confi...
Source: Tissue Engineering and Regenerative Medicine - February 3, 2018 Category: Biotechnology Source Type: research

Combined Treatment with Methylprednisolone and Human Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate Experimental Autoimmune Encephalomyelitis
AbstractMultiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system. Although advances have been made in the treatment of MS, such as the use of IFN- β, glucocorticoids and stem cells, the therapeutic effects of these treatments are not sufficient. In the present study, we evaluated whether the combination of methylprednisolone (MP) and human bone marrow-derived mesenchymal stem cells (BM-MSCs) could enhance the therapeutic effectiveness in expe rimental autoimmune encephalomyelitis (EAE), a model for MS. EAE was induced by immunizing C57BL/6 mice with myelin oligodendrocyte glycopro...
Source: Tissue Engineering and Regenerative Medicine - January 25, 2018 Category: Biotechnology Source Type: research

Correction to: New Advances in Human X Chromosome Status from a Developmental and Stem Cell Biology
AbstractUnfortunately, Acknowledgements section was missing in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Correction to: Engineered M13 Nanofiber Accelerates Ischemic Neovascularization by Enhancing Endothelial Progenitor Cells
AbstractThere is a minor spelling error in the last of name of the 9th author in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Osteogenic Nanofibrous Coated Titanium Implant Results in Enhanced Osseointegration: In Vivo Preliminary Study in a Rabbit Model
AbstractA titanium implant surface when coated with biodegradable, highly porous, osteogenic nanofibrous coating has shown enhanced intrinsic osteoinductive and osteoconductive properties. This coating mimics extracellular matrix resulting in differentiation of stem cells present in the peri-implant niche to osteoblast and hence results in enhanced osseointegration of the implant. The osteogenic nanofibrous coating (ONFC) consists of poly-caprolactone, gelatin, nano-sized hydroxyapatite, dexamethasone, ascorbic acid and beta-glycerophosphate. ONFC exhibits optimum mechanical properties to support mesenchymal stem cells and...
Source: Tissue Engineering and Regenerative Medicine - January 19, 2018 Category: Biotechnology Source Type: research

Correction to: Extracellular Vesicles in Neurodegenerative Diseases: A Double-Edged Sword
AbstractUnfortunately, Acknowledgements section was missing in the originally published article. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 18, 2018 Category: Biotechnology Source Type: research

Adipose Stem Cells with Conditioned Media for Treatment of Acne Vulgaris Scar
In conclusion, the ASCs with CM has a significant curative effect on acne vulgaris scar, more to the point, the C M has a key role on treatment. It could be applied to a therapeutic approach to regenerate to treat acne vulgaris scar. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Feeder Cells Free Rabbit Oral Mucosa Epithelial Cell Sheet Engineering
AbstractThe optimal cell culture method of autologous oral mucosal epithelial cell sheet is not well established for a safe transplantation on to the patients ’ ocular surface. Animal serum and 3T3 mouse feeder cells are currently being used to stimulate the growth of the epithelial cells. However, the use of animal compounds can have potential side effects for the patient after transplantation of the engineered cell sheet. In the present study, we focu sed on engineering a rabbit oral mucosal epithelial cell sheet without 3T3 mouse feeder cells using a mix of Dulbecco’s Modified Eagle Medium/Bronchial Epitheli...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Stem Cell Tracing Through MR Molecular Imaging
AbstractStem cell therapy opens a new window in medicine to overcome several diseases that remain incurable. It appears such diseases as cardiovascular disorders, brain injury, multiple sclerosis, urinary system diseases, cartilage lesions and diabetes are curable with stem cell transplantation. However, some questions related to stem cell therapy have remained unanswered. Stem cell imaging allows approval of appropriated strategies such as selection of the type and dose of stem cell, and also mode of cell delivery before being tested in clinical trials. MRI as a non-invasive imaging modality provides proper conditions for...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Preparation of Drug Eluting Natural Composite Scaffold Using Response Surface Methodology and Artificial Neural Network Approach
AbstractSilk fibroin/xanthan composite was investigated as a suitable biomedical material for controlled drug delivery, and blending ratios of silk fibroin and xanthan were optimized by response surface methodology (RSM) and artificial neural network (ANN) approach. A non-linear ANN model was developed to predict the effect of blending ratios, percentage swelling and porosity of composite material on cumulative percentage release. The efficiency of RSM was assessed against ANN and it was found that ANN is better in optimizing and modeling studies for the fabrication of the composite material.In-vitro release studies of the...
Source: Tissue Engineering and Regenerative Medicine - January 16, 2018 Category: Biotechnology Source Type: research

Effects of Hexachlorophene, a Chemical Accumulating in Adipose Tissue, on Mouse and Human Mesenchymal Stem Cells
AbstractThe hexachlorophene (HCP) is a highly lipophilic chlorinated bisphenol present in hygienic and dermatological products. The HCP accumulates preferentially in adipose tissue that is a privileged source of mesenchymal stem cells (MSCs). The evaluation of the potential effects of HCP on MSCs is important for their medical application. Here we examined the effects of HCP on murine adipose tissue-derived stem cells (ADSCs) and human umbilical cord-derived stem cells (UCSCs) in cell culture. We found that 10−4 and 10−5 M HCP inhibits proliferation, osteogenesis and increases apoptosis of ADSCs and UCSCs....
Source: Tissue Engineering and Regenerative Medicine - January 8, 2018 Category: Biotechnology Source Type: research

Laminin and Platelet-Derived Growth Factor-BB Promote Neuronal Differentiation of Human Urine-Derived Stem Cells
In conclusion, USCs are noteworthy cell source in the field of neu ronal regeneration and laminin and PDGF-BB promote their neuronal differentiation efficiency. (Source: Tissue Engineering and Regenerative Medicine)
Source: Tissue Engineering and Regenerative Medicine - December 28, 2017 Category: Biotechnology Source Type: research

Development of Printable Natural Cartilage Matrix Bioink for 3D Printing of Irregular Tissue Shape
In this study, cartilage acellular matrix (CAM)-based bioink was developed to fabricate functional biomolecule-containing scaffolds. The CAM provides an adequate cartilage tissue –favorable environment for chondrogenic differentiation of cells. Conventional manufacturing techniques such as salt leaching, solvent casting, gas forming, and freeze drying when applied to CAM-based scaffolds cannot precisely control the scaffold geometry for mimicking tissue shape. As an altern ative to the scaffold fabrication methods, 3D printing was recently introduced in the field of tissue engineering. 3D printing may better control ...
Source: Tissue Engineering and Regenerative Medicine - December 28, 2017 Category: Biotechnology Source Type: research

Induction of Rhesus Keratinocytes into Functional Ameloblasts by Mouse Embryonic Dental Mesenchyme
AbstractFast progresses in stem cell-based tooth tissue engineering have been achieved in recent years in several animal models including the mouse, rat, dog, and pig. Moreover, various postnatal mesenchymal stem cells of dental origin have been isolated and shown capable of differentiating into odontoblasts and generating dentin. Meanwhile, human keratinocyte stem/progenitor cells, gingival epithelial cells, and even iPSC-derived epithelium have been demonstrated to be able to differentiate into functional ameloblasts. Translational medicine studies in the nonhuman primate are irreplaceable steps towards clinical applicat...
Source: Tissue Engineering and Regenerative Medicine - December 20, 2017 Category: Biotechnology Source Type: research

The Application of Fibrin/Hyaluronic Acid –Poly( l -Lactic- co -Glycolic Acid) Construct in Augmentation Rhinoplasty
This study evaluated the utility of allogenic chondrocytes with fibrin/hyaluronic acid (HA) –poly(l-lactic-co-glycolic acid) (PLGA) constructs in augmentation rhinoplasty. Chondrocytes from rabbit auricular cartilage were isolated and cultured with fibrin/HA hydrogels and implanted into PLGA scaffolds. After 8  weeks ofin vitro culture, the scaffolds were implanted in the nasal dorsum of six rabbits. Eight weeks postoperatively, the implanted sites were evaluated with gross, radiologic, and histologic analysis.In vitro, more than 90% of the seeded chondrocytes in the PLGA scaffolds survived for 2  weeks, an...
Source: Tissue Engineering and Regenerative Medicine - November 24, 2017 Category: Biotechnology Source Type: research