Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor
Cumulative progress in nanoparticle development has opened a new era of targeted delivery of therapeutics to cancer cells and tissue. However, developing proper detection methods has lagged behind resulting in the lack of precise evaluation and monitoring of the systemically administered nanoparticles. RNA nanoparticles derived from the bacteriophage phi29 DNA packaging motor pRNA have emerged as a new generation of drugs for cancer therapy. Multifunctional RNA nanoparticles can be fabricated by bottom-up self-assembly of engineered RNA fragments harboring targeting (RNA aptamer or chemical ligand), therapeutic (siRNA, miRNA, ribozymes, and small molecule drugs), and imaging (fluorophore, radiolabels) modules. We have recently demonstrated that RNA nanoparticles can reach and target intracranial brain tumors in mice upon systemic injection with little or no accumulation in adjacent healthy brain tissues or in major healthy internal organs. Herein, we describe various functional imaging methods (fluorescence confocal microscopy, flow cytometry, fluorescence whole body imaging, and magnetic resonance imaging) to evaluate and monitor RNA nanoparticle targeting to intracranial brain tumors in mice. Such imaging techniques will allow in-depth evaluation of specifically delivered RNA therapeutics to brain tumors.
In conclusion, we suggested that valproate enhanced 3-BrPA-induced cell death. This might be attributable to the increase in cellular ATP consumption owing to valproate-induced MRP2 or BCRP expression.Graphical abstract
Jordan Payne, 23, from Ripley in Derbyshire, is battling a golf ball-sized brain tumour that is made of germ cells - created as a foetus grows in the womb. They eventually become sperm or eggs.
(Osaka University) Researchers in collaboration with Osaka University found that brain tumors in mice and humans cause immune T-cells to become trapped in bone marrow. This occurs prior to cancer treatment and explains the low circulating levels of T-cells seen in some tumor patients. The effects that brain tumors have on the cell surface S1P1 protein appear to mediate the process. These findings open the door for possible adjuvant cancer treatments.
Georgetown Lombardi Comprehensive Cancer Center has released a set of brain...Read more on AuntMinnie.comRelated Reading: FLT-PET points toward survival for glioblastoma patients SNMMI: FET-PET could have answers for brainstem glioma PET/CT helps assess pediatric brain tumor therapy Brain MRI surprises with incidental findings SNMMI: FDOPA-PET/MRI monitors pediatric brain tumors
(University of Leeds) Aggressive brain tumour cells taken from patients self-destructed after being exposed to a chemical in laboratory tests, researchers have shown.
(Duke University Medical Center) Researchers at Duke Cancer Institute have tracked the missing T-cells in glioblastoma patients. They found them in abundance in the bone marrow, locked away and unable to function because of a process the brain stimulates in response to glioblastoma, to other tumors that metastasize in the brain and even to injury.
SummaryThe response of tumor intracellular pH to a pharmacological challenge could help identify aggressive cancer. Chemical exchange saturation transfer (CEST) is an MRI contrast mechanism that is dependent on intracellular pH (pHi). pHi is important in the maintenance of normal cell function and is normally maintained within a narrow range by the activity of transporters located at the plasma membrane. In cancer, changes in pHi have been correlated with both cell proliferation and cell death. Quercetin is a bioflavonoid and monocarboxylate transporter (MCT) inhibitor. Since MCTs plays a significant role in maintaining pH...
In this report, we present the case of a 5-year-old female with GBM and CMMRD due to an MSH6 homozygous c.1883G>A mutation, who continues to experience an exceptional and durable response (9 months) to the immune checkpoint inhibitor (ICPI) nivolumab. Our patient presented with acute neurologic decline and increased intracranial pressure. Neuroimaging studies revealed a large left frontoparietal mass requiring neurosurgical decompression and resection. Histopathologic analyses resulted in a diagnosis of de novo GBM that was BRAF wild type and negative for programmed death-ligand 1 protein expression. She received standa...
Title: Childhood Brain Tumor Treatment May Hamper Adult SurvivorsCategory: Health NewsCreated: 8/9/2018 12:00:00 AMLast Editorial Review: 8/10/2018 12:00:00 AM