Filtered By:
Specialty: Urology & Nephrology

This page shows you your search results in order of date. This is page number 18.

Order by Relevance | Date

Total 430 results found since Jan 2013.

Negative regulation of Smad1 pathway and collagen IV expression by store-operated Ca2+ entry in glomerular mesangial cells
Collagen IV (Col IV) is a major component of expanded glomerular extracellular matrix in diabetic nephropathy and Smad1 is a key molecule regulating Col IV expression in mesangial cells (MCs). The present study was conducted to determine if Smad1 pathway and Col IV protein abundance were regulated by store-operated Ca2+ entry (SOCE). In cultured human MCs, pharmacological inhibition of SOCE significantly increased the total amount of Smad1 protein. Activation of SOCE blunted high-glucose-increased Smad1 protein content. Treatment of human MCs with ANG II at 1 µM for 15 min, high glucose for 3 days, or TGF-β1 at ...
Source: AJP: Renal Physiology - June 4, 2017 Category: Urology & Nephrology Authors: Wu, P., Ren, Y., Ma, Y., Wang, Y., Jiang, H., Chaudhari, S., Davis, M. E., Zuckerman, J. E., Ma, R. Tags: RESEARCH ARTICLE Source Type: research

Role for reactive oxygen species in flow stimulated inner medullary collecting duct endothelin-1 production.
Abstract Inner medullary collecting duct (IMCD)-derived endothelin-1 (ET-1) is stimulated by volume expansion, in part through augmented luminal flow, whereupon it can elicit natriuresis and diuresis. Since flow can alter nitric oxide (NO) and reactive oxygen species (ROS), both of which can affect collecting duct salt transport, we asked whether NO and/or ROS mediate flow-stimulated IMCD ET-1. Mouse IMCD3 cells were exposed to flow and ET-1/GAPDH mRNA assessed. A shear stress of 10 dyne/cm2 for 1 hr increased ET-1 mRNA by 4-fold compared to no flow (ET-1 flow response). Global NO synthase (NOS) inhibition (L-NAME...
Source: Am J Physiol Renal P... - May 17, 2017 Category: Urology & Nephrology Authors: Wheatley W, Kohan DE Tags: Am J Physiol Renal Physiol Source Type: research

Re: Mg(II)-Catechin Nanoparticles Delivering siRNA Targeting EIF5A2 Inhibit Bladder Cancer Cell Growth In Vitro and In Vivo
Z. Chen, T. Yu, B. Zhou, J. Wei, Y. Fang, J. Lu, L. Guo, W. Chen, Z. P. Liu and J. Luo
Source: The Journal of Urology - May 10, 2017 Category: Urology & Nephrology Authors: Anthony Atala Tags: Urological Survey Source Type: research

Beneficial Effects of Myo-Inositol Oxygenase Deficiency in Cisplatin-Induced AKI
Overexpression of the proximal tubular enzyme myo-inositol oxygenase (MIOX) induces oxidant stress in vitro. However, the relevance of MIOX to tubular pathobiology remains enigmatic. To investigate the role of MIOX in cisplatin-induced tubular AKI, we generated conditional MIOX-overexpressing transgenic (MIOX-TG) mice and MIOX-knockout (MIOX–/–) mice with tubule-specific MIOX overexpression or knockout, respectively. Compared with cisplatin-treated wild-type (WT) mice, cisplatin-treated MIOX-TG mice had even greater increases in urea, creatinine, and KIM-1 levels and more tubular injury and apoptosis, but these...
Source: Journal of the American Society of Nephrology : JASN - April 28, 2017 Category: Urology & Nephrology Authors: Dutta, R. K., Kondeti, V. K., Sharma, I., Chandel, N. S., Quaggin, S. E., Kanwar, Y. S. Tags: Basic Research Source Type: research

Fibrinogen links podocyte injury with Toll ‐like receptor 4 and is associated with disease activity in FSGS patients
ConclusionsFg induced podocytes injury via the TLR4‐p38 MAPK‐NF‐κB p65 pathway. In FSGS patients, urinary Fg levels reflect therapeutic response to prednisone and disease activity.
Source: Nephrology - April 13, 2017 Category: Urology & Nephrology Authors: Hongtian Wang, Chunxia Zheng, Xiaodong Xu, Yue Zhao, Yinghui Lu, Zhihong Liu Tags: Original Article Source Type: research

NLRP3 inflammasome activation contributes to aldosterone-induced podocyte injury
Aldosterone (Aldo) has been shown as an important contributor of podocyte injury. However, the underlying molecular mechanisms are still elusive. Recently, the pathogenic role of NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome in mediating renal tubular damage was identified while its role in podocyte injury still needs evidence. Thus the present study was undertaken to investigate the role of NLRP3 inflammasome in Aldo-induced podocyte damage. In vitro, exposure of podocytes to Aldo enhanced NLRP3, caspase-1, and IL-18 expressions in dose- and time-dependent manners, indicating an activation of NL...
Source: AJP: Renal Physiology - April 1, 2017 Category: Urology & Nephrology Authors: Bai, M., Chen, Y., Zhao, M., Zhang, Y., He, J. C.-J., Huang, S., Jia, Z., Zhang, A. Tags: RESEARCH ARTICLE Source Type: research

Elevated PTH induces endothelial-to-chondrogenic transition in aortic endothelial cells
Previous studies have shown that increased parathyroid hormone (PTH) attributable to secondary hyperparathyroidism in chronic kidney disease accelerates the arteriosclerotic fibrosis and calcification. Although the underlying mechanisms remain largely unknown, endothelial cells (ECs) have recently been demonstrated to participate in calcification in part by providing chondrogenic cells via the endothelial-to-mesenchymal transition (EndMT). Therefore, this study aimed to investigate whether elevated PTH could induce endothelial-to-chondrogenic transition in aortic ECs and to determine the possible underlying signaling pathw...
Source: AJP: Renal Physiology - February 28, 2017 Category: Urology & Nephrology Authors: Wu, M., Zhang, J.-D., Tang, R.-N., Crowley, S. D., Liu, H., Lv, L.-L., Ma, K.-L., Liu, B.-C. Tags: RESEARCH ARTICLE Source Type: research

Elevation of SHARPIN Protein Levels in Prostate Adenocarcinomas Promotes Metastasis and Impairs Patient Survivals
CONCLUSIONSSHARPIN enhances the metastasis of prostate cancer and impair patient survivals. Prostate. © 2017 Wiley Periodicals, Inc.
Source: The Prostate - February 23, 2017 Category: Urology & Nephrology Authors: Hai Huang, Tao Du, Yiming Zhang, Yiming Lai, Kaiwen Li, Xinxing Fan, Dingjun Zhu, Tianxin Lin, Kewei Xu, Jian Huang, Leyuan Liu, Zhenghui Guo Tags: Original Article Source Type: research

Androgen Receptor Splice Variants Are Not Substrates of Nonsense ‐Mediated Decay
CONCLUSIONThis study eliminates a possible mechanism of regulation of certain AR variants. Future research into the regulation of AR variants should focus on other mechanisms to better understand the origin of these variants and to possibly inhibit their expression for the resensitization of resistant cancers. Prostate. © 2017 Wiley Periodicals, Inc.
Source: The Prostate - February 22, 2017 Category: Urology & Nephrology Authors: Atinuke S. Ajiboye, David Esopi, Srinivasan Yegnasubramanian, Samuel R. Denmeade Tags: Original Article Source Type: research

Delayed treatment with fenofibrate protects against high-fat diet-induced kidney injury in mice: the possible role of AMPK autophagy
Fenofibrate activates not only peroxisome proliferator-activated receptor-α (PPARα) but also adenosine monophosphate-activated protein kinase (AMPK). AMPK-mediated cellular responses protect kidney from high-fat diet (HFD)-induced injury, and autophagy resulting from AMPK activation has been regarded as a stress-response mechanism. Thus the present study examined the role of AMPK and autophagy in the renotherapeutic effects of fenofibrate. C57BL/6J mice were divided into three groups: normal diet (ND), HFD, and HFD + fenofibrate (HFD + FF). Fenofibrate was administered 4 wk after the initiation of the HFD when ...
Source: AJP: Renal Physiology - February 7, 2017 Category: Urology & Nephrology Authors: Sohn, M., Kim, K., Uddin, M. J., Lee, G., Hwang, I., Kang, H., Kim, H., Lee, J. H., Ha, H. Tags: RESEARCH ARTICLE Source Type: research

STAT5 drives abnormal proliferation in autosomal dominant polycystic kidney disease
Autosomal dominant polycystic kidney disease (ADPKD) leads to renal failure. The hallmark of ADPKD is increased epithelial proliferation, which has been proposed to be due to atypical signaling including abnormal JAK-STAT activity. However, the relative contribution of JAK-STAT family members in promoting proliferation in ADPKD is unknown. Here, we present siRNA JAK-STAT –focused screens discovering a previously unknown proliferative role for multiple JAK-STAT components (including STAT1, STAT2, STAT4, STAT5a, and STAT5b).
Source: Kidney International - January 15, 2017 Category: Urology & Nephrology Authors: Maria Fragiadaki, Morgane Lannoy, Madeleine Themanns, Barbara Maurer, Wouter N. Leonhard, Dorien J.M. Peters, Richard Moriggl, Albert C.M. Ong Tags: Basic Research Source Type: research

Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway
ConclusionSimulated physiological stretch increases the expression of ECM proteins in HBSMCs, and integrin α4/αv-FAK-ERK1/2 signaling pathway partly modulates the mechano-transducing process.
Source: World Journal of Urology - December 23, 2016 Category: Urology & Nephrology Source Type: research

ILK and cytoskeletal architecture: an important determinant of AQP2 recycling and subsequent entry into the exocytotic pathway
Within the past decade tremendous efforts have been made to understand the mechanism behind aquaporin-2 (AQP2) water channel trafficking and recycling, to open a path toward effective diabetes insipidus therapeutics. A recent study has shown that integrin-linked kinase (ILK) conditional-knockdown mice developed polyuria along with decreased AQP2 expression. To understand whether ILK also regulates AQP2 trafficking in kidney tubular cells, we performed in vitro analysis using LLCPK1 cells stably expressing rat AQP2 (LLC-AQP2 cells). Upon treatment of LLC-AQP2 cells with ILK inhibitor cpd22 and ILK-siRNA, we observed increas...
Source: AJP: Renal Physiology - December 11, 2016 Category: Urology & Nephrology Authors: Mamuya, F. A., Cano-Penalver, J. L., Li, W., Rodriguez Puyol, D., Rodriguez Puyol, M., Brown, D., de Frutos, S., Lu, H. A. J. Tags: CALL FOR PAPERS Source Type: research

Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2
The carboxyl terminus of aquaporin-2 (AQP2c) undergoes posttranslational modifications, including phosphorylation and ubiquitination, in the process of regulating aquaporin-2 (AQP2) translocation and protein abundance. We aimed to identify novel proteins interacting with AQP2c. Recombinant AQP2c protein was made in Escherichia coli BL21 (DE3) cells by exploiting the pET32 TrxA fusion system. Lysates of rat kidney inner medullary collecting duct (IMCD) tubule suspensions interacted with rat AQP2c bound to Ni2+-resin were subjected to LC-MS/MS proteomic analysis. Potential interacting proteins were identified, including vacu...
Source: AJP: Renal Physiology - November 30, 2016 Category: Urology & Nephrology Authors: Lee, M. S., Choi, H.-J., Park, E.-J., Park, H.-J., Kwon, T.-H. Tags: ARTICLES Source Type: research

Simvastatin Up ‐Regulates Annexin A10 That Can Inhibit the Proliferation, Migration, and Invasion in Androgen‐Independent Human Prostate Cancer Cells
CONCLUSIONOur results suggest that statins inhibit the proliferation, migration, and invasion of androgen‐independent prostate cancer cells by up‐regulation of ANXA10. Additionally, it is possible that S100A4 plays a role in these effects. Statins may be beneficial in the prevention and/or treatment of prostate cancer. Prostate © 2016 Wiley Periodicals, Inc.
Source: The Prostate - November 7, 2016 Category: Urology & Nephrology Authors: Yoshiyuki Miyazawa, Yoshitaka Sekine, Haruo Kato, Yosuke Furuya, Hidekazu Koike, Kazuhiro Suzuki Tags: Original Article Source Type: research