Mutation of ROR γT reveals a role for Th17 cells in both injury and recovery from renal ischemia reperfusion injury.
Mutation of RORγT reveals a role for Th17 cells in both injury and recovery from renal ischemia reperfusion injury. Am J Physiol Renal Physiol. 2020 Sep 14;: Authors: Mehrotra P, Ullah MM, Collett JA, Myers SL, Dwinell MR, Geurts AM, Basile DP Abstract To investigate Th17 cells in the setting of acute kidney injury (AKI), the master regulator of Th17 cell differentiation, RORγT, was mutated in Lewis rats using CRISPR/Cas9 technology. In response to 40 min bilateral renal I/R, Rorc-/- rats were resistant to injury relative to wild-type Rorc+/+ rats. This protection was associated with inhib...
Source: Am J Physiol Renal P... - September 14, 2020 Category: Urology & Nephrology Authors: Mehrotra P, Ullah MM, Collett JA, Myers SL, Dwinell MR, Geurts AM, Basile DP Tags: Am J Physiol Renal Physiol Source Type: research

Distal Convoluted Tubule Sexual Dimorphism Revealed by Advanced 3D-Imaging.
Abstract The thiazide-sensitive sodium chloride cotransporter (NCC) is more abundant in kidneys of females than of males. Because morphological remodeling of the distal convoluted tubule (DCT) is dependent on NCC activity, it has been generally assumed that there is a corresponding sexual dimorphism in the structure of the DCT, leading to a larger female DCT. Until now, this has never been directly examined. Here, optical clearing techniques were combined with antibody labeling of DCT segment markers, state-of-the-art high-speed volumetric imaging and analysis tools to visualize and quantify DCT morphology in male...
Source: Am J Physiol Renal P... - September 14, 2020 Category: Urology & Nephrology Authors: Tahaei E, Coleman R, Saritas T, Ellison DH, Welling PA Tags: Am J Physiol Renal Physiol Source Type: research

A minireview of pharmacologic strategies used to ameliorate polyuria associated with X-linked nephrogenic diabetes insipidus.
Abstract Nephrogenic diabetes insipidus (NDI) is characterized by renal resistance to the antidiuretic hormone arginine vasopressin (AVP) which leads to polyuria, plasma hyperosmolarity, polydipsia and impaired quality of living. Inherited forms are caused by X-linked loss-of-function mutations in the gene encoding the vasopressin 2- receptor (V2R) or autosomal mutations in the gene encoding aquaporin 2 (AQP2). A common acquired form is lithium-induced NDI. AVP facilitates reabsorption of water through increased abundance and insertion of AQP2 in the apical membrane of the principal cells in the collecting ducts. ...
Source: Am J Physiol Renal P... - September 14, 2020 Category: Urology & Nephrology Authors: Mortensen LA, Bistrup C, Jensen BL, Hinrichs GR Tags: Am J Physiol Renal Physiol Source Type: research

G Protein-coupled Estrogen Receptor as a Novel Regulator of Blood Pressure.
Abstract The mechanisms underlying hypertension are multi-faceted and incompletely understood. New evidence suggests that the G protein-coupled estrogen receptor 1 (GPER1) mediates protective actions within the cardiovascular and renal systems. This minireview focuses on recent advancements in our understanding of the vascular, renal, and cardiac GPER1-mediated mechanisms that influence blood pressure regulation. We emphasize clinical and basic evidence that suggests GPER1 as a novel target to aid therapeutic strategies for hypertension. Furthermore, we discuss current controversies and challenges facing GPER1-rel...
Source: Am J Physiol Renal P... - September 7, 2020 Category: Urology & Nephrology Authors: Gohar EY Tags: Am J Physiol Renal Physiol Source Type: research

A role for the tubular Na+-H+-exchanger NHE3 in the natriuretic effect of the SGLT2 inhibitor empagliflozin.
Abstract Inhibitors of the proximal tubular sodium glucose cotransporter SGLT2 are natriuretic and they lower blood pressure. There are reports that the activities of SGLT2 and the Na+-H+-exchanger, NHE3, are coordinated. If so, then part of the natriuretic response to an SGLT2 inhibitor is mediated by suppressing NHE3. To examine this further, we compared the effects of the SGLT2 inhibitor, empagliflozin, on urine composition and systolic blood pressure (SBP) in non-diabetic mice with tubule-specific NHE3 knock-down (NHE3-ko) and wild-type littermates (WT). A single dose of empagliflozin, titrated to cause minima...
Source: Am J Physiol Renal P... - September 7, 2020 Category: Urology & Nephrology Authors: Onishi A, Fu Y, Patel R, Darshi M, Crespo-Masip M, Huang W, Song P, Freeman B, Kim YC, Soleimani M, Sharma K, Thomson SC, Vallon V Tags: Am J Physiol Renal Physiol Source Type: research

Praliciguat inhibits progression of diabetic nephropathy in ZSF1 rats and suppresses inflammation and apoptosis in human renal proximal tubular cells.
In conclusion, praliciguat improved proteinuria in the ZSF1 rat model of DN, and its actions in human RPTC suggest that tubular effects may contribute to its renal benefits, building upon strong evidence for the role of cGMP signaling in renal health. PMID: 32865013 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - August 31, 2020 Category: Urology & Nephrology Authors: Liu G, Shea CM, Jones JE, Price GM, Warren W, Lonie E, Yan S, Currie MG, Profy AT, Masferrer JL, Zimmer DP Tags: Am J Physiol Renal Physiol Source Type: research

A (pro)renin receptor decoy peptide PRO20 protects against adriamycin-induced nephropathy by targeting intrarenal renin-angiotensin system.
Abstract Adriamycin (ADR) administration in susceptible rodents such as the BALB/c mouse strain produces injury to the glomerulus mimicking human chronic kidney disease (CKD) due to primary focal segmental glomerulosclerosis. The goal of the present study was to use this model to investigate antiproteinuric action of (pro)renin receptor (PRR) decoy inhibitor PRO20. BALB/c mice were pretreated for 1 day with PRO20 at 500 μg/kg/day via an osmotic minipump, followed by a single injection of vehicle or ADR (10 mg/kg) via the tail vein. Albuminuria and renal function were analyzed at 4th week post-ADR administration...
Source: Am J Physiol Renal P... - August 31, 2020 Category: Urology & Nephrology Authors: Luo R, Yang KT, Wang F, Xu C, Yang T Tags: Am J Physiol Renal Physiol Source Type: research

Soluble (pro)renin receptor promotes fibrotic response in renal proximal tubule epithelial cells in vitro via Akt/ β-catenin/Snail signaling pathway.
Soluble (pro)renin receptor promotes fibrotic response in renal proximal tubule epithelial cells in vitro via Akt/β-catenin/Snail signaling pathway. Am J Physiol Renal Physiol. 2020 Aug 31;: Authors: Xie S, Su J, Lu A, Lai Y, Mo S, Pu M, Yang T Abstract Tubulointerstitial fibrosis has been regarded as a critical event in the pathogenesis of chronic kidney disease (CKD). Soluble form of PRR (sPRR), generated by site-1 protease (S1P) cleavage of full-length PRR, is detected in biological fluid and elevated under certain pathological conditions. The present study was designed to evaluate the potenti...
Source: Am J Physiol Renal P... - August 31, 2020 Category: Urology & Nephrology Authors: Xie S, Su J, Lu A, Lai Y, Mo S, Pu M, Yang T Tags: Am J Physiol Renal Physiol Source Type: research

Vitamin K Deficiency: An Emerging Player in the Pathogenesis of Vascular Calcification and an Iatrogenic Consequence of Therapies in Advanced Renal Disease.
Abstract Vascular calcification is a known complication of chronic kidney disease (CKD). The prevalence of vascular calcification in patients with non-dialysis-dependent CKD stages 3-5 has been shown to be as high as 79%. [1] Vascular calcification has been associated with increased risk for mortality, hospital admissions, and cardiovascular disease. [1] [2] [3] [4] Alterations in mineral and bone metabolism play a pivotal role in the pathogenesis of vascular calcification in CKD. As CKD progresses, levels of fibroblast growth factor-23 (FGF-23), parathyroid hormone (PTH) and serum phosphorus increase and 1,25-(OH...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Levy D, Grewal R, Le TH Tags: Am J Physiol Renal Physiol Source Type: research

Protein Disulfide Isomerase Inhibition Impairs Keap1/Nrf2 Signaling and Mitochondrial Function and Induces Apoptosis in Renal Proximal Tubular Cells.
Abstract Renal proximal tubular apoptosis plays a critical role in kidney health and disease. However, cellular molecules that trigger renal apoptosis remain elusive. Here, we evaluated the effect of inhibiting protein disulfide isomerase (PDI), a critical thioredoxin chaperone protein, on apoptosis, and the underlying mechanisms in human renal proximal tubular (HK2) cells. HK2 cells were transfected with PDI specific siRNA in the absence and presence of an antioxidant tempol. PDI siRNA transfection resulted in a decrease of ~70% in PDI protein expression and enzyme activity. PDI inhibition increased caspase-3 act...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Pokkunuri ID, Lokhandwala MF, Banday AA Tags: Am J Physiol Renal Physiol Source Type: research

MAD2B contributes to parietal epithelial cell activation and crescentic glomerulonephritis via Skp2.
Abstract Mitotic spindle assembly checkpoint protein 2 (MAD2B), a well-known anaphase-promoting complex/cyclosome (APC/C) inhibitor and a small subunit of DNA polymerase ζ, is critical for mitotic control and DNA repair. Previously, we detected a strong increase of MAD2B in the glomeruli from crescentic glomerulonephritis patients and anti-glomerular basement membrane (anti-GBM) rats, which predominantly originated from activated parietal epithelial cells (PECs). Consistently, in vitro MAD2B was increased in tumor necrosis factor-α (TNF-α)-treated PECs, along with cell activation and proliferation...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Ye C, Xiong W, Lei CT, Tang H, Su H, Yi F, Zhang C Tags: Am J Physiol Renal Physiol Source Type: research

(Pro)renin receptor promotes crescent formation via the ERK1/2 and Wnt/ β-catenin pathways in glomerulonephritis.
(Pro)renin receptor promotes crescent formation via the ERK1/2 and Wnt/β-catenin pathways in glomerulonephritis. Am J Physiol Renal Physiol. 2020 Aug 24;: Authors: Urushihara M, Kondo S, Kinoshita Y, Ozaki N, Jamba A, Nagai T, Fujioka K, Hattori T, Kagami S Abstract (Pro)renin receptor ((P)RR) has multiple functions, but its regulation and role in the pathogenesis in glomerulonephritis (GN) are poorly defined. The aims of this study were to determine the effects of direct renin inhibition (DRI) and demonstrate the role of (P)RR on the progression of crescentic GN. Anti-glomerular basement membran...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Urushihara M, Kondo S, Kinoshita Y, Ozaki N, Jamba A, Nagai T, Fujioka K, Hattori T, Kagami S Tags: Am J Physiol Renal Physiol Source Type: research

Expression of Acsm2, a kidney-specific gene, parallels the function and maturation of proximal tubular cells.
In this study, we found that the Acsm2 gene was expressed specifically and at a high level in normal adult kidneys. The expression of Acsm2 in kidneys followed a maturational pattern: it was low in newborn mice and increased with kidney development and maturation. In situ hybridization and immunohistochemistry revealed that Acsm2 was expressed specifically in proximal tubular cells of adult kidneys. Data from the Encyclopedia of DNA Elements database revealed that the Acsm2 gene locus in the mouse has specific histone modifications related to the active transcription of the gene exclusively in kidney cells. Following acute...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Watanabe H, Paxton RL, Tolerico MR, Nagalakshmi VK, Tanaka S, Okusa MD, Goto S, Narita I, Watanabe S, Sequeira-Lοpez MLS, Gomez RA Tags: Am J Physiol Renal Physiol Source Type: research

Impaired Renal Hemodynamics and Glomerular Hyperfiltration Contribute to Hypertension-Induced Renal Injury.
Abstract Recently, we reported a mutation in γ-Adducin (ADD3) was associated with an impaired myogenic response of the afferent arteriole and hypertension-induced chronic kidney disease (CKD) in FHH rats. However, the mechanisms by which altered renal blood flow (RBF) autoregulation promotes hypertension-induced renal injury remains to be determined. The present study compared the time course of changes in renal hemodynamics and the progression of CKD during the development of DOCA-salt hypertension in FHH 1BN congenic rats (WT) with an intact myogenic response versus FHH 1BN Add3KO (Add3KO) rats which have ...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Fan L, Gao W, Nguyen BV, Jefferson JR, Liu Y, Fan F, Roman RJ Tags: Am J Physiol Renal Physiol Source Type: research

C57BL/6 mice require a higher dose of cisplatin to induce renal fibrosis and CCL2 correlates with cisplatin-induced kidney injury.
In this study, we demonstrate that increasing the dose of cisplatin to 9 mg/kg once a week for four weeks is sufficient to consistently induce fibrosis in C57BL/6 mice while maintaining animal survival. In addition, we present that cohorts of C57BL/6 mice purchased from Jackson one year apart and mice bred in house display variability in renal outcomes following repeated low dose cisplatin treatment. In depth analyses of this intra-animal variability revealed CCL2 as a marker of cisplatin-induced kidney injury through correlation studies. In addition, significant immune cell infiltration was observed in the kidney after fo...
Source: Am J Physiol Renal P... - August 24, 2020 Category: Urology & Nephrology Authors: Sears SM, Sharp CN, Krueger AM, Oropilla GB, Saforo D, Doll MA, Megyesi J, Beverly LJ, Siskind LJ Tags: Am J Physiol Renal Physiol Source Type: research

Intracellular location of aquaporin-2 serine 269 phosphorylation and dephosphorylation in kidney collecting duct cells.
Abstract Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for water reabsorption by the kidney collecting ducts. Under control conditions, most AQP2 resides in the recycling endosomes of the principal cells where it answers to vasopressin with trafficking to the apical plasma membrane to increase water reabsorption. Upon vasopressin withdrawal, apical AQP2 retreats to the early endosomes before joining the recycling endosomes for the next vasopressin stimulation. Prior studies have demonstrated a role of AQP2 S269 phosphorylation in reducing AQP2 endocytosis thereby prolonging apical...
Source: Am J Physiol Renal P... - August 17, 2020 Category: Urology & Nephrology Authors: Wong KY, Wang WL, Su SH, Liu CF, Yu MJ Tags: Am J Physiol Renal Physiol Source Type: research

Intratubular epithelial-mesenchymal transition and tubular atrophy after kidney injury in mice.
In conclusion, injury-induced intratubular EMT is closely related to tubular atrophy in a FAK-dependent manner. PMID: 32799673 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - August 17, 2020 Category: Urology & Nephrology Authors: Yamashita N, Kusaba T, Nakata T, Tomita A, Ida T, Watanabe-Uehara N, Ikeda K, Kitani T, Uehara M, Kirita Y, Matoba S, Humphreys BD, Tamagaki K Tags: Am J Physiol Renal Physiol Source Type: research

COX-2-Independent Activation of Renal (Pro)Renin Receptor Contributes to DOCA-Salt Hypertension in Rats.
Abstract It's been shown that COX-2-dependent activation of renal (pro)renin receptor (PRR) contributes to angiotensin II (AngII)-induced hypertension. However, less is known about the involvement of this mechanism in AngII-independent hypertension. The goal of the present study was to test whether or not COX-2-dependent upregulation of PRR serves as a universal mechanism contributing to AngII-dependent and -independent hypertension. Here we examined the association between renal COX-2 and PRR during deoxycorticosterone acetate (DOCA)-salt hypertension in rats. By immunoblotting and immunofluorescence, renal prote...
Source: Am J Physiol Renal P... - August 17, 2020 Category: Urology & Nephrology Authors: Wang F, Sun Y, Luo R, Lu X, Yang B, Yang T Tags: Am J Physiol Renal Physiol Source Type: research

Early antihypertensive treatment and ischemia-induced acute kidney injury.
Conclusions - Early antihypertensive treatment worsened renal outcome after AKI by further reducing renal perfusion despite reduced glomerulosclerosis. PMID: 32799675 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - August 17, 2020 Category: Urology & Nephrology Authors: Greite R, Derlin K, Hensen B, Thorenz A, Rong S, Chen R, Hellms S, Jang MS, Bräsen JH, Meier M, Willenberg I, Immenschuh S, Haller H, Luft FC, Panigrahy D, Hwang SH, Hammock BD, Schebb NH, Gueler F Tags: Am J Physiol Renal Physiol Source Type: research

Distal Convoluted Tubule Chloride Concentration is Modulated via Potassium Channels and Transporters.
Abstract Cl--sensitive with-no-lysine kinase (WNK) plays a key role in regulating the thiazide-sensitive sodium-chloride cotransporter (NCC) in the distal convoluted tubule (DCT). Cl- enters DCT cells through NCC and leaves the cell across the basolateral membrane via the Cl- channel ClC-Kb or a K-Cl cotransporter (KCC). While KCC is electroneutral, Cl- exit via ClC-Kb is electrogenic. Therefore, an alteration in DCT basolateral K+ channel activity is expected to influence Cl- movement across the basolateral membrane. Although a role for intracellular Cl- in the regulation of WNK and NCC has been established, intr...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Su XT, Klett NJ, Sharma A, Allen CN, Wang WH, Yang CL, Ellison DH Tags: Am J Physiol Renal Physiol Source Type: research

Systemic Inhibition of miR-451 Increases Fibrotic Signaling and Diminishes Autophagic Response to Exacerbate Renal Damage in Tallyho/Jng Mice.
Abstract MicroRNAs (miRs) provide fine-tuning of gene expression via inhibition of translation. MiR-451 has a modulatory role in cell cycling via down-regulation of the mechanistic target of rapamycin (mTOR). We aimed to test whether chronic, systemic inhibition of miR-451 would enhance renal fibrosis (associated with deranged autophagy). Adult, TallyHo/Jng mice (obese, insulin-resistant) were randomized to two treatment groups to receive either miR-451 inhibition (via a locked nucleic acid, LNA, construct) or a similar scrambled-LNA control for 8 weeks. All mice were fed a high-fat diet (60% kcal from fat) ad lib...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Fluitt MB, Shivapurkar N, Kumari M, Singh S, Li L, Tiwari S, Ecelbarger CM Tags: Am J Physiol Renal Physiol Source Type: research

Protective Role of DJ-1 in Endotoxin-induced Acute Kidney Injury.
In this study we examined the effect of DJ-1 deficiency in a murine model of endotoxin-induced AKI. Endotoxemia induced greater kidney injury in DJ-1-deficient mice. Further, DJ-1 deficiency increased renal oxidative stress associated with increased renal tubular apoptosis and with expression of death domain-associated protein (DAXX). Similar to the in vivo model, in vitro studies, using a medullary collecting duct cell line (IMCD3) and cytotoxic serum, we show that serum obtained from WT mice results in increased expression of s100A8/s100A9, DAXX, and apoptosis in DJ-1 deficient iMCD3 cells. Our findings demonstrate a nov...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Leeds J, Scindia Y, Loi V, Wlazlo E, Ghias E, Cechova S, Portilla D, Ledesma J, Swaminathan S Tags: Am J Physiol Renal Physiol Source Type: research

PGF2 α stimulates 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule.
PGF2α stimulates 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule. Am J Physiol Renal Physiol. 2020 Jul 27;: Authors: Wang LJ, Xiao Y, Fang J, Wang JL, Zhang H, Meng XX, Gong RL, Gu R Abstract We used patch-clamp and western blot to test whether PGF2α stimulates the basolateral 10-pS Cl channel and thiazide-sensitive Na-Cl cotransporter (NCC) in distal convoluted tubule (DCT) via FP receptor (FP-R). The single-channel and whole cell recordings demonstrated that PGF2α stimulated the 10-pS Cl channel in DCT. The stimulatory effect of PGF2&al...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Wang LJ, Xiao Y, Fang J, Wang JL, Zhang H, Meng XX, Gong RL, Gu R Tags: Am J Physiol Renal Physiol Source Type: research

Virtual Measurements of Paracellular Permeability and Chronic Inflammation Via Color Coded Pixel-wise T1 Mapping.
Abstract To assess whether quantitative T1 relaxometry can measure permeability, assess chronic inflammation and mural thickening of mouse bladder wall. Adult female C57BL6 mice unexposed to radiation (controls) or 40 weeks post-irradiation10Gy were scanned at 9.4T before and after instillation (0.1mL) of aqueous, novel contrast mixture containing 4mM Gadobutrol and 5mM Ferumoxytol. Rapid Acquisition with Refocused Echo (RARE) sequence with variable Repetition Times (TR) generated pixel wise map of T1 relaxation times for segmented bladder wall layers from voxel-wise, nonlinear least square data fitting of TR depe...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Singh N, Zabbarova I, Ikeda Y, Maranchie J, Chermansky C, Foley LM, Hitchens TK, Yoshimura N, Kanai AJ, Kaufman J, Tyagi P Tags: Am J Physiol Renal Physiol Source Type: research

Liraglutide suppresses production of extracellular matrix proteins and ameliorates renal injury of diabetic nephropathy by enhancing Wnt/ β-catenin signaling.
Liraglutide suppresses production of extracellular matrix proteins and ameliorates renal injury of diabetic nephropathy by enhancing Wnt/β-catenin signaling. Am J Physiol Renal Physiol. 2020 Jul 27;: Authors: Huang L, Lin T, Shi M, Chen X, Wu P Abstract The Wnt/β-catenin signaling pathway is involved in the production of extracellular matrix (ECM) by mesangial cells (MCs). Recent studies by us and others demonstrated that glucagon-like peptide-1 receptor agonists (GLP-1RAs) have protective effects against diabetic nephropathy. The purpose of this study was to investigate whether the Wnt/&bet...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Huang L, Lin T, Shi M, Chen X, Wu P Tags: Am J Physiol Renal Physiol Source Type: research

Endothelial prolyl hydroxylase 2 is necessary for Angiotensin II-mediated renal fibrosis and injury.
Abstract Angiotensin II (Ang II) is the key contributor to renal fibrosis and injury. The present study is to investigate the role of endothelium prolyl hydroxylase 2 (PHD2) in Ang II-mediated renal fibrosis and injury. In vitro, endothelial cells (ECs) were isolated from PHD2f/f control (wild type, WT) mice or PHD2 EC knockout (PHD2ECKO) mice. In vivo, WT and PHD2ECKO mice were infused with Ang II (1000ng/kg/min) for 28 days. Renal fibrosis, reactive oxygen species (ROS) and iron contents were measured. Knockout of PHD2 resulted in a significant increase in expression of HIF-1 and HIF-2 in ECs. Intriguingly...
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Zhao Y, Zeng H, Liu B, He X, Chen JX Tags: Am J Physiol Renal Physiol Source Type: research

Decreased secretion and profibrotic activity of tubular exosomes in diabetic kidney disease.
In conclusion, DKD is associated with a decrease in exosome secretion in renal tubular cells. The exosomes from high glucose conditioned tubular cells may regulate the proliferation and activation of fibroblasts, contributing to the paracrine signaling mechanism responsible for the pathological onset of renal interstitial fibrosis in DKD. PMID: 32715764 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 27, 2020 Category: Urology & Nephrology Authors: Wen J, Ma Z, Livingston MJ, Zhang W, Yuan Y, Guo C, Liu Y, Fu P, Dong Z Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +31 new citations
31 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2020/07/18PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 18, 2020 Category: Urology & Nephrology Tags: Report Source Type: research

Am J Physiol Renal Physiol; +41 new citations
41 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2020/07/16PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 16, 2020 Category: Urology & Nephrology Tags: Report Source Type: research

Gene expression profiling of proximal and distal renal tubules in Atlantic salmon (Salmo salar) acclimated to fresh water and seawater.
This study aimed to obtain a broader understanding of transcriptional regulation in proximal vs. distal tubular segments during osmotic transitions. Proximal and distal tubule cells were dissected separately by laser capture micro-dissection, RNA was extracted, and relative mRNA expression level of>30 targets involved in solute and water transport were quantified by qPCR in relation to segment type in fish acclimated to FW or SW. The gene categories were aquaporins, solute transporters, fxyd proteins and tight junction proteins. aqp8bb1, aqp10b1, nhe3, sglt1, slc41a1, cnnm3, fxyd12a, cldn3b, cldn10b, cldn15a and cldn12 ...
Source: Am J Physiol Renal P... - July 6, 2020 Category: Urology & Nephrology Authors: Madsen SS, Bollinger RJ, Brauckhoff M, Engelund MB Tags: Am J Physiol Renal Physiol Source Type: research

Urinary bladder smooth muscle ion channels: expression, function, and regulation in health and disease.
Abstract The urinary bladder smooth muscle (UBSM), also known as detrusor smooth muscle, forms the bladder wall and ultimately determines the two main attributes of the organ: urine storage and voiding. The two functions are facilitated by UBSM relaxation and contraction, respectively, which depend on UBSM excitability shaped by multiple ion channels. In this review, we summarize the current understanding of key ion channels establishing and regulating UBSM excitability and contractility. They include excitation-enhancing voltage-gated Ca2+ (CaV) and transient receptor potential (TRP) channels, excitation-reducing...
Source: Am J Physiol Renal P... - July 6, 2020 Category: Urology & Nephrology Authors: Malysz J, Petkov GV Tags: Am J Physiol Renal Physiol Source Type: research

Aldosterone-dependent and -independent regulation of Na and K excretion and ENaC in mouse kidney.
Abstract We investigated the regulation of Na+and K+ excretion and ENaC in mice lacking the gene for aldosterone synthase (AS), using clearance methods to assess excretion and electrophysiology and Western blotting to test for ENaC activity and processing. After one day of dietary Na restriction AS-/- mice lost more Na+ in the urine than did AS+/+. After 1 week on this diet both genotypes strongly reduced urinary Na+ excretion, but creatinine clearance decreased only in AS-/- mice. Only AS+/+ animals exhibited increased ENaC function, assessed as amiloride-sensitive whole-cell currents in collecting ducts or the c...
Source: Am J Physiol Renal P... - July 6, 2020 Category: Urology & Nephrology Authors: Yang L, Frindt G, Xu Y, Uchida S, Palmer LG Tags: Am J Physiol Renal Physiol Source Type: research

MiR-21 promotes calcium oxalate-induced renal tubular cell injury by targeting PPARA.
Abstract Kidney stone disease is a crystal concretion formed in kidneys which has been associated with an increased risk of chronic kidney disease. MicroRNAs are functionally involved in kidney injury. Data mining using a microRNA array database suggested that miR-21 may be associated with calcium oxalate monohydrate (COM)-induced renal tubular cell injury. Here, we confirmed that COM exposure significantly upregulated miR-21 expression, inhibited proliferation, promoted apoptosis and caused lipid accumulation in the immortalized renal tubular cell line HK-2. Moreover, inhibition of miR-21 enhanced proliferation a...
Source: Am J Physiol Renal P... - July 6, 2020 Category: Urology & Nephrology Authors: Su B, Han H, Ji C, Hu W, Yao J, Yang J, Fan Y, Li J Tags: Am J Physiol Renal Physiol Source Type: research

Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
This study demonstrates the potential of the IMPRes method to identify novel pathways. Identifying the "Proteoglycans in cancer" and "Galactose metabolism" pathways generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix. PMID: 32628542 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 6, 2020 Category: Urology & Nephrology Authors: Srivastava T, Joshi T, Jiang Y, Heruth DP, Rezaiekhaligh MH, Novak J, Staggs VS, Alon US, Garola RE, El-Meanawy A, McCarthy ET, Zhou J, Boinpelly VC, Sharma R, Savin VJ, Sharma M Tags: Am J Physiol Renal Physiol Source Type: research

Sex differences in renal ischemia/reperfusion injury.
e; AJ Abstract Ischemia/reperfusion injury of the kidney is caused by the sudden and temporary obstruction of blood flow to the organ. Renal ischemia/reperfusion injury is associated with high morbidity and mortality, but effective therapies are lacking. Sexual dimorphism in renal injury has been acknowledged since the 1940s and the possible role of sex hormones has been intensively investigated in the past decades. Clinical and experimental data demonstrate sexual differences in renal anatomy, physiology and susceptibility to renal diseases including, but not limited to ischemia/reperfusion injury. Some data sugg...
Source: Am J Physiol Renal P... - June 22, 2020 Category: Urology & Nephrology Authors: Hosszu A, Fekete A, Szabó AJ Tags: Am J Physiol Renal Physiol Source Type: research

Quantitative intravital calcium imaging maps single cell behavior to kidney tubular structure.
Abstract Ca2+ is an important second messenger that translates extracellular stimuli into intracellular responses. Although there has been significant progress in understanding Ca2+ dynamics in organs such as the brain, the nature of Ca2+ signals in the kidney is still poorly understood. Here, we show that by using a genetically expressed highly sensitive reporter (GCaMP6s) it is possible to perform imaging of Ca2+ signals at high resolution in the mouse kidney in vivo. Moreover, by applying machine learning based automated analysis using a Ca2+-independent signal, quantitative data can be extracted in an unbiased...
Source: Am J Physiol Renal P... - June 22, 2020 Category: Urology & Nephrology Authors: Martins J, Haenni D, Bugarski M, Figurek A, Hall AM Tags: Am J Physiol Renal Physiol Source Type: research

Rab-rabphilin system in injured human podocytes stressed by glucose overload and angiotensin ii.
RAB-RABPHILIN SYSTEM IN INJURED HUMAN PODOCYTES STRESSED BY GLUCOSE OVERLOAD AND ANGIOTENSIN II. Am J Physiol Renal Physiol. 2020 Jun 22;: Authors: Martinez-Arroyo O, Ortega A, Perez-Hernandez J, Chaves FJ, Redon J, Cortes R Abstract Kidney injury in hypertension and diabetes entails, among in other structures, damage in a key cell of the glomerular filtration barrier, the podocyte. Podocytes are polarized and highly differentiated cells in which vesicular transport, partly driven by Rab GTPases, is a relevant process. The present aim was to analyze Rab GTPases of the Rab-Rabphilin system in human imm...
Source: Am J Physiol Renal P... - June 22, 2020 Category: Urology & Nephrology Authors: Martinez-Arroyo O, Ortega A, Perez-Hernandez J, Chaves FJ, Redon J, Cortes R Tags: Am J Physiol Renal Physiol Source Type: research

A two-stage bilateral ischemia reperfusion injury-induced AKI to CKD transition model in mice.
In conclusion, a new bilateral AKI to CKD transition animal model with typical phenotype of CKD was generated in C57BL/6 mice. PMID: 32567350 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 22, 2020 Category: Urology & Nephrology Authors: Zhang J, Wang X, Wei J, Wang L, Jiang S, Xu L, Qu L, Yang K, Fu L, Buggs J, Cheng F, Liu R Tags: Am J Physiol Renal Physiol Source Type: research

An acute rise of plasma sodium concentration associates with syndecan-1 shedding during hemodialysis.
Abstract Endothelial dysfunction (ED) contributes to the high incidence of cardiovascular events in hemodialysis patients. Syndecan-1 in the endothelial glycocalyx can be shed into the circulation serving as a biomarker for ED. As sodium is a trigger for glycocalyx shedding, we now tested whether hemodialysis with higher dialysate sodium concentrations is associated with more syndecan-1 shedding compared with standard hemodialysis (SHD). In this cross-over study in 29 patients, plasma syndecan-1 was repeatedly measured during SHD and during Hemocontrol hemodialysis (HHD) which is characterized by initially higher ...
Source: Am J Physiol Renal P... - June 15, 2020 Category: Urology & Nephrology Authors: Koch J, Idzerda NMA, Ettema EM, Kuipers H, Dam W, van den Born J, Franssen CF Tags: Am J Physiol Renal Physiol Source Type: research

Chronic unilateral cervical vagotomy reduces renal inflammation, blood pressure, and renal injury in a mouse model of lupus.
CONCLUSION: In summary, these data indicate that unilateral vagotomy reduces renal inflammation and reduces blood pressure in SLE mice. The vagus nerves have myriad functions and perhaps other neuroimmune interactions compensate for the ligation of one nerve. PMID: 32538149 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 15, 2020 Category: Urology & Nephrology Authors: Pham GS, Gusson Shimoura C, Chaudhari S, Kulp DV, Mathis KW Tags: Am J Physiol Renal Physiol Source Type: research

Evolution of Altered Tubular Metabolism and Mitochondrial Function in Sepsis Associated Acute Kidney Injury.
In conclusion, we demonstrate significant alterations in renal oxygenation, tubular mitochondrial function and metabolic reprogramming in s-AKI which may play an important role in the progression of injury and recovery from AKI in sepsis. PMID: 32538150 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 15, 2020 Category: Urology & Nephrology Authors: Li Y, Nourbakhsh N, Pham H, Tham R, Zuckerman JE, Singh P Tags: Am J Physiol Renal Physiol Source Type: research

Molecular Targeting of Renal Inflammation using Drug Delivery Technology to Inhibit NF-kB Improves Renal Recovery in Chronic Kidney Disease.
Abstract Inflammation is a major determinant for the progression of chronic kidney disease (CKD). Nuclear-factor-kappa-B (NF-kB) is a master transcription factor up-regulated in CKD that promotes inflammation, regulates apoptosis, and vascular remodeling. We aimed to modulate this pathway for CKD therapy in a swine model of CKD using a peptide inhibitor of the NF-kB p50 subunit (p50i) fused to a protein carrier (elastin-like polypeptide, ELP) and equipped with a cell-penetrating peptide (SynB1). We hypothesize that intra-renal SynB1-ELP-p50i therapy will inhibit NF-kB-driven inflammation and induce renal recovery....
Source: Am J Physiol Renal P... - June 15, 2020 Category: Urology & Nephrology Authors: Chade AR, Williams ML, Engel JE, Williams E, Bidwell GL Tags: Am J Physiol Renal Physiol Source Type: research

Ultrasound for the Treatment of Acute Kidney Injury and other Inflammatory Conditions: A Promising Path Toward Noninvasive Neuroimmune Regulation.
Abstract Acute kidney injury (AKI) is an important clinical disorder with high prevalence, serious consequences and limited therapeutic options. Modulation of neuro-immune interaction by nonpharmacological methods is emerging as a novel strategy for treating inflammatory diseases, including AKI. Recently, pulsed ultrasound (US) treatment was shown to protect from AKI by stimulating the cholinergic anti-inflammatory pathway (CAP). Due to the relatively simple, portable, and noninvasive nature of US procedures, US-stimulation may be a valuable therapeutic option for treating inflammatory conditions. This review disc...
Source: Am J Physiol Renal P... - June 8, 2020 Category: Urology & Nephrology Authors: Cai J, Nash WT, Okusa MD Tags: Am J Physiol Renal Physiol Source Type: research

1,3-Butanediol attenuates hypertension and suppresses kidney injury in female rats.
This study tests the hypothesis that 1,3-Butanediol attenuates hypertension and progression of CKD in female S-SHR(11). S-SHR(11) is a congenic rat strain generated from genetic modification of the Dahl S rat, previously characterized as a model of accelerated renal disease. Rats received 1,3-Butanediol (20% via drinking water) or control for 10 weeks and were maintained on a 0.3% NaCl rodent diet (n=12-14/group). Blood pressure was measured after 6 and 9 weeks of treatment by tail-cuff plethysmography, and after 10 weeks urine and tissues were collected. Activity of the treatment was confirmed by measuring plasma β-h...
Source: Am J Physiol Renal P... - June 8, 2020 Category: Urology & Nephrology Authors: Ishimwe JA, Garrett MR, Sasser JM Tags: Am J Physiol Renal Physiol Source Type: research

Pharmacokinetics (PK) and pharmacodynamics (PD) of TTI-101, a STAT3 inhibitor that blocks muscle proteolysis in rats with chronic kidney disease.
Abstract Loss of muscle proteins increases the morbidity and mortality of patients with chronic kidney disease (CKD) and there are no reliable preventive treatments. We uncovered a STAT3, CEBPδ to myostatin signaling pathway that activates muscle protein degradation in mice with CKD or cancer; we also identified a small molecule inhibitor of STAT3 (TTI-101) that blocks this pathway. To evaluate TTI-101 as a treatment of CKD-induced cachexia, we measured TTI-101 pharmacokinetics (PK) and pharmacodynamics (PD) in control and CKD rats that were orally administrated TTI-101or its diluent. Two groups of gavage-fe...
Source: Am J Physiol Renal P... - June 1, 2020 Category: Urology & Nephrology Authors: Zhang L, Wang Y, Dong Y, Chen Z, Eckols TK, Kasembeli MM, Tweardy DJ, Mitch WE Tags: Am J Physiol Renal Physiol Source Type: research

Chronic elevation of renal venous pressure induces extensive renal venous collateral formation and modulates renal function and cardiovascular stability in rats.
Abstract Acutely increased renal venous pressure (RVP) impairs renal function but long-term impact is unknown. We investigated whether chronic RVP elevation impairs baseline renal function and prevents exacerbation of renal dysfunction and cardiovascular instability upon further RVP increase. RVP elevation (20-25mmHg) or sham was performed in rats. After 1 (n=17) or 3 weeks (n=22), blood pressure, RVP, renal blood flow (RBF), renal vascular conductance (RVC) and glomerular filtration rate (GFR) were measured at baseline and during superimposed RVP increase. Chronic RVP elevation induced extensive renal venous coll...
Source: Am J Physiol Renal P... - June 1, 2020 Category: Urology & Nephrology Authors: Hamza SM, Huang X, Zehra T, Zhuang W, Cupples WA, Braam B Tags: Am J Physiol Renal Physiol Source Type: research

In vitro efficacy and safety of a system for sorbent assisted peritoneal dialysis.
CONCLUSIONS: SAPD treatment may improve removal of uremic toxins compared with conventional PD, provided that peritoneal mass transport will increase. PMID: 32475132 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 1, 2020 Category: Urology & Nephrology Authors: van Gelder MK, Ligabue G, Giovanella S, Bianchini E, Simonis F, Hazenbrink DHM, Joles JA, Bajo Rubio MA, Selgas R, Cappelli G, Gerritsen KGF Tags: Am J Physiol Renal Physiol Source Type: research

Long non-coding RNA NEAT1 sponges miR-129 to modulate renal fibrosis by regulation of collagen I.
In conclusion, NEAT1 sponged miR-129 to modulate EMT process and inflammation response of renal fibrosis by regulation of collagen I. Our study indicated a novel role in the regulation of renal fibrosis and provided a new potential treatment target for renal fibrosis. PMID: 32475133 [PubMed - as supplied by publisher] (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - June 1, 2020 Category: Urology & Nephrology Authors: Li C, Liu YF, Huang C, Chen YX, Xu CY, Chen Y Tags: Am J Physiol Renal Physiol Source Type: research

A non-invasive method to study evolution of extracellular fluid volume in mice using time domain nuclear magnetic resonance.
We examined the feasibility of monitoring mice ECF by a non-invasive method, using time-domain nuclear magnetic resonance (TD-NMR). This technique allows differentiating protons in a liquid environment (free fluid) from protons in soft tissues containing a majority of either small molecules (lean) or large molecules (fat). Moreover, this apparatus enables rapid, non-invasive, and repeated measurements on the same animal. We assessed the feasibility of coupling TD-NMR analysis to a longitudinal metabolic cage study by monitoring mice daily. We determined the effect of a 24-hour water deprivation on mice body parameters and ...
Source: Am J Physiol Renal P... - June 1, 2020 Category: Urology & Nephrology Authors: Morla L, Shore O, Lynch IJ, Merritt ME, Wingo CS Tags: Am J Physiol Renal Physiol Source Type: research

Am J Physiol Renal Physiol; +29 new citations
29 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Physiol Renal Physiol These pubmed results were generated on 2020/05/13PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites. (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - May 13, 2020 Category: Urology & Nephrology Tags: Report Source Type: research