Bayesian Identification of Candidate Transcription Factors for Regulation of Aqp2 Gene Expression
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00204.2021. Online ahead of print.ABSTRACTAqp2 gene transcription is strongly regulated by vasopressin in the renal collecting duct. However, the transcription factors (TFs) responsible for regulation of expression of aquaporin-2 (Aqp2) remain largely unknown. We used Bayes' Theorem to integrate several -omics datasets to stratify the 1344 TFs present in the mouse genome with regard to probabilities of regulating Aqp2 gene transcription. Also, we carried out new RNA-Seq experiments mapping the time course of vasopressin-induced changes in the transcriptome of m...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Hiroaki Kikuchi Hyun Jun Jung Viswanathan Raghuram Kirby T Leo Euijung Park Chin-Rang Yang Chung-Lin Chou Lihe Chen Mark A Knepper Source Type: research

Mechanisms of decreased tubular flow-induced nitric oxide in Dahl salt-sensitive rat thick ascending limbs
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00124.2021. Online ahead of print.ABSTRACTDahl salt-sensitive rat (SS) kidneys produce less nitric oxide (NO) than those of salt-resistant rats (SR). Thick ascending limb (THAL) NO synthase 3 (NOS3) is a major source of renal NO, and luminal flow enhances its activity. We hypothesized that flow-induced NO is reduced in SS THALs primarily due to NOS uncoupling and diminished NOS3 expression rather than scavenging. Rats were fed normal (NS) or high-salt (HS) diets. We measured flow-induced NO and superoxide in perfused THALs, and performed Western blots of renal ...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Nancy J Hong Agustin Gonzalez-Vicente Fara Saez Jeffrey L Garvin Source Type: research

Podocyte endowment and the impact of adult body size on kidney health
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00029.2021. Online ahead of print.ABSTRACTLow birth weight is a risk factor for chronic kidney disease (CKD), while adult podocyte depletion is a key event in the pathogenesis of glomerulosclerosis. However, whether low birth weight due to poor maternal nutrition is associated with low podocyte endowment and glomerulosclerosis in later life is not known. Female Sprague-Dawley rats were fed a normal (NPD, 20%) or low (LPD, 8%) protein diet from 3 weeks before mating until postnatal day 21 (PN21), when kidneys from some male offspring were taken for quantitation ...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Luise Anne Cullen-McEwen James van der Wolde Kotaro Haruhara Leon Tribolet John Dowling Michael Bertram Robert De Matteo Fabian Haas Jan Czogalla Yusuke Okabayashi James Armitage M Jane Black Wendy E Hoy Victor Puelles John F Bertram Source Type: research

Bayesian Identification of Candidate Transcription Factors for Regulation of Aqp2 Gene Expression
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00204.2021. Online ahead of print.ABSTRACTAqp2 gene transcription is strongly regulated by vasopressin in the renal collecting duct. However, the transcription factors (TFs) responsible for regulation of expression of aquaporin-2 (Aqp2) remain largely unknown. We used Bayes' Theorem to integrate several -omics datasets to stratify the 1344 TFs present in the mouse genome with regard to probabilities of regulating Aqp2 gene transcription. Also, we carried out new RNA-Seq experiments mapping the time course of vasopressin-induced changes in the transcriptome of m...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Hiroaki Kikuchi Hyun Jun Jung Viswanathan Raghuram Kirby T Leo Euijung Park Chin-Rang Yang Chung-Lin Chou Lihe Chen Mark A Knepper Source Type: research

Mechanisms of decreased tubular flow-induced nitric oxide in Dahl salt-sensitive rat thick ascending limbs
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00124.2021. Online ahead of print.ABSTRACTDahl salt-sensitive rat (SS) kidneys produce less nitric oxide (NO) than those of salt-resistant rats (SR). Thick ascending limb (THAL) NO synthase 3 (NOS3) is a major source of renal NO, and luminal flow enhances its activity. We hypothesized that flow-induced NO is reduced in SS THALs primarily due to NOS uncoupling and diminished NOS3 expression rather than scavenging. Rats were fed normal (NS) or high-salt (HS) diets. We measured flow-induced NO and superoxide in perfused THALs, and performed Western blots of renal ...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Nancy J Hong Agustin Gonzalez-Vicente Fara Saez Jeffrey L Garvin Source Type: research

Podocyte endowment and the impact of adult body size on kidney health
Am J Physiol Renal Physiol. 2021 Jul 26. doi: 10.1152/ajprenal.00029.2021. Online ahead of print.ABSTRACTLow birth weight is a risk factor for chronic kidney disease (CKD), while adult podocyte depletion is a key event in the pathogenesis of glomerulosclerosis. However, whether low birth weight due to poor maternal nutrition is associated with low podocyte endowment and glomerulosclerosis in later life is not known. Female Sprague-Dawley rats were fed a normal (NPD, 20%) or low (LPD, 8%) protein diet from 3 weeks before mating until postnatal day 21 (PN21), when kidneys from some male offspring were taken for quantitation ...
Source: Am J Physiol Renal P... - July 26, 2021 Category: Urology & Nephrology Authors: Luise Anne Cullen-McEwen James van der Wolde Kotaro Haruhara Leon Tribolet John Dowling Michael Bertram Robert De Matteo Fabian Haas Jan Czogalla Yusuke Okabayashi James Armitage M Jane Black Wendy E Hoy Victor Puelles John F Bertram Source Type: research

A crosstalk between HIF and NF- κB in AKI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00256.2021. Online ahead of print.NO ABSTRACTPMID:34282955 | DOI:10.1152/ajprenal.00256.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Zhiwen Liu Zheng Dong Source Type: research

Characterization of a functional V < sub > 1B < /sub > vasopressin receptor in the male rat kidney, evidence for crosstalk between V < sub > 1B < /sub > and V < sub > 2 < /sub > receptor signaling pathways
This study brings new data on the localization and signaling pathways of V1B-R in the kidney, highlights a crosstalk between V1B-R and V2-R in IMCD and suggests that V1B-R may counterbalance in some pathophysiological conditions the antidiuretic effect triggered by V2-R activation.PMID:34282956 | DOI:10.1152/ajprenal.00081.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Annette Hus-Citharel Nadine Bouby Ma ïthé Corbani Julie Mion Christiane Mendre Judit Darusi Csaba Tomboly Miguel Trueba Claudine Serradeil-Le Gal Catherine Llorens-Cortes Gilles Guillon Source Type: research

Image analysis techniques to map pyramids, pyramid structure, glomerular distribution, and pathology in the intact human kidney from 3D MRI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00130.2021. Online ahead of print.ABSTRACTKidney pathologies are often highly heterogenous. To comprehensively understand kidney structure and pathology, it is critical to develop tool to map tissue microstructure in the context of the whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional (3D) view of the kidney and allows for measurement of multiple pathologic features. Here, we develop a platform to systematically render and map gross and microstructural features of the human kidney based on 3D MRI. These features inclu...
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Jennifer R Charlton Yanzhe Xu Neda Parvin Teresa Wu Fei Gao Edwin J Baldelomar Darya Morozov Scott C Beeman Jamal Derakhshan Kevin M Bennett Source Type: research

First Author Spotlight
Am J Physiol Renal Physiol. 2021 Jul 1;321(1):i. doi: 10.1152/ajprenal.2021.321.1.AU.NO ABSTRACTPMID:34283638 | DOI:10.1152/ajprenal.2021.321.1.AU (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Source Type: research

A crosstalk between HIF and NF- κB in AKI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00256.2021. Online ahead of print.NO ABSTRACTPMID:34282955 | DOI:10.1152/ajprenal.00256.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Zhiwen Liu Zheng Dong Source Type: research

Characterization of a functional V < sub > 1B < /sub > vasopressin receptor in the male rat kidney, evidence for crosstalk between V < sub > 1B < /sub > and V < sub > 2 < /sub > receptor signaling pathways
This study brings new data on the localization and signaling pathways of V1B-R in the kidney, highlights a crosstalk between V1B-R and V2-R in IMCD and suggests that V1B-R may counterbalance in some pathophysiological conditions the antidiuretic effect triggered by V2-R activation.PMID:34282956 | DOI:10.1152/ajprenal.00081.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Annette Hus-Citharel Nadine Bouby Ma ïthé Corbani Julie Mion Christiane Mendre Judit Darusi Csaba Tomboly Miguel Trueba Claudine Serradeil-Le Gal Catherine Llorens-Cortes Gilles Guillon Source Type: research

Image analysis techniques to map pyramids, pyramid structure, glomerular distribution, and pathology in the intact human kidney from 3D MRI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00130.2021. Online ahead of print.ABSTRACTKidney pathologies are often highly heterogenous. To comprehensively understand kidney structure and pathology, it is critical to develop tool to map tissue microstructure in the context of the whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional (3D) view of the kidney and allows for measurement of multiple pathologic features. Here, we develop a platform to systematically render and map gross and microstructural features of the human kidney based on 3D MRI. These features inclu...
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Jennifer R Charlton Yanzhe Xu Neda Parvin Teresa Wu Fei Gao Edwin J Baldelomar Darya Morozov Scott C Beeman Jamal Derakhshan Kevin M Bennett Source Type: research

First Author Spotlight
Am J Physiol Renal Physiol. 2021 Jul 1;321(1):i. doi: 10.1152/ajprenal.2021.321.1.AU.NO ABSTRACTPMID:34283638 | DOI:10.1152/ajprenal.2021.321.1.AU (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Source Type: research

A crosstalk between HIF and NF- κB in AKI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00256.2021. Online ahead of print.NO ABSTRACTPMID:34282955 | DOI:10.1152/ajprenal.00256.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Zhiwen Liu Zheng Dong Source Type: research

Characterization of a functional V < sub > 1B < /sub > vasopressin receptor in the male rat kidney, evidence for crosstalk between V < sub > 1B < /sub > and V < sub > 2 < /sub > receptor signaling pathways
This study brings new data on the localization and signaling pathways of V1B-R in the kidney, highlights a crosstalk between V1B-R and V2-R in IMCD and suggests that V1B-R may counterbalance in some pathophysiological conditions the antidiuretic effect triggered by V2-R activation.PMID:34282956 | DOI:10.1152/ajprenal.00081.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Annette Hus-Citharel Nadine Bouby Ma ïthé Corbani Julie Mion Christiane Mendre Judit Darusi Csaba Tomboly Miguel Trueba Claudine Serradeil-Le Gal Catherine Llorens-Cortes Gilles Guillon Source Type: research

Image analysis techniques to map pyramids, pyramid structure, glomerular distribution, and pathology in the intact human kidney from 3D MRI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00130.2021. Online ahead of print.ABSTRACTKidney pathologies are often highly heterogenous. To comprehensively understand kidney structure and pathology, it is critical to develop tool to map tissue microstructure in the context of the whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional (3D) view of the kidney and allows for measurement of multiple pathologic features. Here, we develop a platform to systematically render and map gross and microstructural features of the human kidney based on 3D MRI. These features inclu...
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Jennifer R Charlton Yanzhe Xu Neda Parvin Teresa Wu Fei Gao Edwin J Baldelomar Darya Morozov Scott C Beeman Jamal Derakhshan Kevin M Bennett Source Type: research

First Author Spotlight
Am J Physiol Renal Physiol. 2021 Jul 1;321(1):i. doi: 10.1152/ajprenal.2021.321.1.AU.NO ABSTRACTPMID:34283638 | DOI:10.1152/ajprenal.2021.321.1.AU (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Source Type: research

A crosstalk between HIF and NF- κB in AKI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00256.2021. Online ahead of print.NO ABSTRACTPMID:34282955 | DOI:10.1152/ajprenal.00256.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Zhiwen Liu Zheng Dong Source Type: research

Characterization of a functional V < sub > 1B < /sub > vasopressin receptor in the male rat kidney, evidence for crosstalk between V < sub > 1B < /sub > and V < sub > 2 < /sub > receptor signaling pathways
This study brings new data on the localization and signaling pathways of V1B-R in the kidney, highlights a crosstalk between V1B-R and V2-R in IMCD and suggests that V1B-R may counterbalance in some pathophysiological conditions the antidiuretic effect triggered by V2-R activation.PMID:34282956 | DOI:10.1152/ajprenal.00081.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Annette Hus-Citharel Nadine Bouby Ma ïthé Corbani Julie Mion Christiane Mendre Judit Darusi Csaba Tomboly Miguel Trueba Claudine Serradeil-Le Gal Catherine Llorens-Cortes Gilles Guillon Source Type: research

Image analysis techniques to map pyramids, pyramid structure, glomerular distribution, and pathology in the intact human kidney from 3D MRI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00130.2021. Online ahead of print.ABSTRACTKidney pathologies are often highly heterogenous. To comprehensively understand kidney structure and pathology, it is critical to develop tool to map tissue microstructure in the context of the whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional (3D) view of the kidney and allows for measurement of multiple pathologic features. Here, we develop a platform to systematically render and map gross and microstructural features of the human kidney based on 3D MRI. These features inclu...
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Jennifer R Charlton Yanzhe Xu Neda Parvin Teresa Wu Fei Gao Edwin J Baldelomar Darya Morozov Scott C Beeman Jamal Derakhshan Kevin M Bennett Source Type: research

First Author Spotlight
Am J Physiol Renal Physiol. 2021 Jul 1;321(1):i. doi: 10.1152/ajprenal.2021.321.1.AU.NO ABSTRACTPMID:34283638 | DOI:10.1152/ajprenal.2021.321.1.AU (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Source Type: research

A crosstalk between HIF and NF- κB in AKI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00256.2021. Online ahead of print.NO ABSTRACTPMID:34282955 | DOI:10.1152/ajprenal.00256.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Zhiwen Liu Zheng Dong Source Type: research

Characterization of a functional V < sub > 1B < /sub > vasopressin receptor in the male rat kidney, evidence for crosstalk between V < sub > 1B < /sub > and V < sub > 2 < /sub > receptor signaling pathways
This study brings new data on the localization and signaling pathways of V1B-R in the kidney, highlights a crosstalk between V1B-R and V2-R in IMCD and suggests that V1B-R may counterbalance in some pathophysiological conditions the antidiuretic effect triggered by V2-R activation.PMID:34282956 | DOI:10.1152/ajprenal.00081.2021 (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Annette Hus-Citharel Nadine Bouby Ma ïthé Corbani Julie Mion Christiane Mendre Judit Darusi Csaba Tomboly Miguel Trueba Claudine Serradeil-Le Gal Catherine Llorens-Cortes Gilles Guillon Source Type: research

Image analysis techniques to map pyramids, pyramid structure, glomerular distribution, and pathology in the intact human kidney from 3D MRI
Am J Physiol Renal Physiol. 2021 Jul 20. doi: 10.1152/ajprenal.00130.2021. Online ahead of print.ABSTRACTKidney pathologies are often highly heterogenous. To comprehensively understand kidney structure and pathology, it is critical to develop tool to map tissue microstructure in the context of the whole, intact organ. Magnetic resonance imaging (MRI) can provide a unique, three-dimensional (3D) view of the kidney and allows for measurement of multiple pathologic features. Here, we develop a platform to systematically render and map gross and microstructural features of the human kidney based on 3D MRI. These features inclu...
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Authors: Jennifer R Charlton Yanzhe Xu Neda Parvin Teresa Wu Fei Gao Edwin J Baldelomar Darya Morozov Scott C Beeman Jamal Derakhshan Kevin M Bennett Source Type: research

First Author Spotlight
Am J Physiol Renal Physiol. 2021 Jul 1;321(1):i. doi: 10.1152/ajprenal.2021.321.1.AU.NO ABSTRACTPMID:34283638 | DOI:10.1152/ajprenal.2021.321.1.AU (Source: Am J Physiol Renal P...)
Source: Am J Physiol Renal P... - July 20, 2021 Category: Urology & Nephrology Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

Critical role of the mineralocorticoid receptor in aldosterone-dependent and aldosterone-independent regulation of ENaC in the distal nephron
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00139.2021. Online ahead of print.ABSTRACTThe epithelial sodium channel (ENaC) constitutes the rate-limiting step for sodium absorption in the aldosterone-sensitive distal nephron (ASDN) comprising the late distal convoluted tubule (DCT2), the connecting tubule (CNT) and the collecting duct. Previously, we demonstrated that ENaC activity in the DCT2/CNT transition zone is constitutively high and independent of aldosterone, in contrast to its aldosterone dependence in the late CNT and initial cortical collecting duct (CNT/CCD). The mineralocorticoid receptor (MR...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Viatcheslav Nesterov Marko Bertog J érémie Canonica Edith Hummler Richard Coleman Paul A Welling Christoph Korbmacher Source Type: research

IFT88 deficiency in proximal tubular cells exaggerates cisplatin-induced injury by suppressing autophagy
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00672.2020. Online ahead of print.ABSTRACTPrimary cilia are widely regarded as specialized sensors in differentiated cells that have been implicated in the regulation of cell proliferation, differentiation, and viability. We previously showed that shortening of primary cilia sensitizes cultured kidney tubular cells to cisplatin-induced apoptosis. IFT88 is an essential component for ciliogenesis and maintenance. Here, we have further examined the effect of proximal tubule-specific IFT88 ablation on cisplatin-induced acute kidney injury (AKI). In the study, more ...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Shixuan Wang Shougang Zhuang Zheng Dong Source Type: research

Ultrabright Plasmonic-Fluor Nanolabel-Enabled Detection of a Urinary ER Stress Biomarker in Autosomal Dominant Tubulointerstitial Kidney Disease
Am J Physiol Renal Physiol. 2021 Jul 12. doi: 10.1152/ajprenal.00231.2021. Online ahead of print.ABSTRACTAutosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin (UMOD) is the most common non-polycystic genetic kidney disease, but it remains unrecognized due to its clinical heterogeneity and lack of screening test. Moreover, clinical feature being a poor predictor of the disease outcome further highlights the need for development of mechanistic biomarkers in ADTKD. However, low abundant urinary proteins secreted by thick ascending limb (TAL) cells, where UMOD is synthesized, have posed a challenge on detecti...
Source: Am J Physiol Renal P... - July 12, 2021 Category: Urology & Nephrology Authors: Yeawon Kim Zheyu Wang Chuang Li Kendrah Kidd Yixuan Wang Bryce Johnson Stanislav Kmoch Jeremiah Morrissey Anthony Bleyer Jeremy Duffield Srikanth Singamaneni Ying Maggie Chen Source Type: research

HIF-1 α is transcriptionally regulated by NF-κB in acute kidney injury
In this study, the mechanism of HIF-α regulation in AKI was investigated. We found that tubular HIF-1α expression significantly increased at the transcriptional level in ischemia/reperfusion (I/R)-, unilateral ureteral obstruction (UUO)-, and sepsis-induced AKI models, which was closely associated with macrophage-dependent inflammation. Meanwhile, nuclear factor-κB (NF-κB), which plays a central role in inflammation response, was involved in the increasing expression of HIF-1α in AKI, as evidenced by pharmacological modulation (NF-κB inhibitor BAY11-7082). Mechanistically, NF-κB di...
Source: Am J Physiol Renal P... - July 7, 2021 Category: Urology & Nephrology Authors: Zuo-Lin Li Jia-Ling Ji Yi Wen Jingyuan Cao Naresh Kharbuja Wei-Jie Ni Di Yin Song-Tao Feng Hong Liu Lin-Li Lv Bi-Cheng Liu Bin Wang Source Type: research

L-WNK1 is required for BK channel activation in intercalated cells
We examined the role of IC L-WNK1 expression in enhancing BK channel activity in response to a high K+ diet. Mice with an IC-selective deletion of L-WNK1 (IC-L-WNK1-KO) and littermate controls were placed on a high K+ (5% K+ as KCl) diet for 10 or more days. IC-L-WNK1-KO mice exhibited reduced IC apical/subapical BK α subunit expression and BK channel-dependent whole-cell currents compared to controls. Six-hour urinary K+ excretion in response a saline load was similar in IC-L-WNK1-KO mice and controls. The observations that IC-L-WNK1-KO mice on a high K+ diet have higher blood [K+] and reduced IC BK channel activity...
Source: Am J Physiol Renal P... - July 7, 2021 Category: Urology & Nephrology Authors: Evan C Ray Rolando Carrisoza-Gayt án Mohammad M Al-Bataineh Allison L Marciszyn Lubika J Nkashama Jingxin Chen Aaliyah Winfrey Shawn E Griffiths Tracey Lam Daniel Flores Peng Wu Wen-Hui Wang Chou-Long Huang Arohan R Subramanya Thomas R Kleyman Lisa M Sat Source Type: research

HIF-1 α is transcriptionally regulated by NF-κB in acute kidney injury
In this study, the mechanism of HIF-α regulation in AKI was investigated. We found that tubular HIF-1α expression significantly increased at the transcriptional level in ischemia/reperfusion (I/R)-, unilateral ureteral obstruction (UUO)-, and sepsis-induced AKI models, which was closely associated with macrophage-dependent inflammation. Meanwhile, nuclear factor-κB (NF-κB), which plays a central role in inflammation response, was involved in the increasing expression of HIF-1α in AKI, as evidenced by pharmacological modulation (NF-κB inhibitor BAY11-7082). Mechanistically, NF-κB di...
Source: Am J Physiol Renal P... - July 7, 2021 Category: Urology & Nephrology Authors: Zuo-Lin Li Jia-Ling Ji Yi Wen Jingyuan Cao Naresh Kharbuja Wei-Jie Ni Di Yin Song-Tao Feng Hong Liu Lin-Li Lv Bi-Cheng Liu Bin Wang Source Type: research