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Specialty: Urology & Nephrology

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Total 430 results found since Jan 2013.

Protective effect of Gelofusine against cRGD-siRNA-induced nephrotoxicity in mice.
Authors: Liao W, Qin Y, Liao L, Cen B, Wu Z, Wei Y, Wang Z, Li G, Ji A Abstract Based on successful targeting to the αvβ3 integrin of cyclic arginine-glycine-aspartic acid (cRGD), cRGD-conjugated small interfering RNA (siRNA) exhibits tumor targeting and has become a new treatment strategy for solid tumors. However, the nephrotoxicity caused by its renal retention limits its clinical application. Here, we evaluated the protective effect of Gelofusine against cRGD-conjugated siRNA-induced nephrotoxicity in mice. Male Kunming mice (six per group) were either co-injected with Gelofusine and cRGD-siRNA or injected wi...
Source: Renal Failure - April 7, 2018 Category: Urology & Nephrology Tags: Ren Fail Source Type: research

Urethral Reconstruction with Small Intestinal Submucosa Seeded with Oral Keratinocytes and TIMP-1 siRNA Transfected Fibroblasts in a Rabbit Model
Background: To evaluate the effect of tissue inhibitor of metalloproteinase-1 small interfering RNA (TIMP-1 siRNA) transfected fibroblasts (FB) for urethral reconstruction. Materials and Methods: A ventral urethral mucosal defect was created. Substitution urethroplasty was performed with small intestinal submucosa (SIS) alone (8 rabbits, group 1), autogenic oral keratinocytes (OK)-seeded SIS (8 rabbits, group 2) or autogenic OK and TIMP-1 siRNA transfected FB-seeded SIS (8 rabbits, group 3). At 1 and 6 months after surgery (4 rabbits at each time point), retrograde urethrogram and histologic analysis were performed to eval...
Source: Urologia Internationalis - October 17, 2015 Category: Urology & Nephrology Source Type: research

Intravenous siRNA silencing of survivin enhances activity of mitomycin C in human bladder RT4 xenografts
Survivin inhibits apoptosis and enables tumor cells to escape from therapy-induced senescence. High expression of survivin is associated with bladder cancer aggressiveness and recurrence. The present study evaluated if survivin expression is reduced by siRNA and if survivin silencing enhances the activity of mitomycin C (MMC), in human RT4 bladder transitional cell tumors in vitro and in vivo.
Source: The Journal of Urology - February 10, 2015 Category: Urology & Nephrology Authors: Minjian Cui, Jessie L.-S. Au, M. Guillaume Wientjes, Michael A. O’Donnell, Kevin R. Loughlin, Ze Lu Source Type: research

Mp46-15 lipid nanoparticle sirna potently silences clusterin and delays progression when combined with androgen receptor co-targeting in enzalutamide resistant prostate cancer
Suppression of androgen receptor (AR) signaling remains a therapeutic goal for castration resistant prostate cancer (CRPC) and despite newer potent AR pathway inhibitors, resistance frequently occurs. While co-targeting the AR with adaptive survival pathways is a rational goal, many biologically relevant genes including clusterin (CLU) are not druggable with small molecule inhibitors. While small interfering RNA (siRNA) offers the promise for very potent and specific gene silencing, in vivo delivery remains a major barrier.
Source: The Journal of Urology - April 1, 2015 Category: Urology & Nephrology Authors: Yoshiaki Yamamoto, Paulo Lin, Fan Zhang, Eliana Beraldi, Yoshihisa Kawai, Jeffrey Leong, Hideyasu Matsuyama, Pieter Cullis, Martin Gleave Tags: Prostate Cancer: Basic Research II Source Type: research

PLK-1 Silencing in Bladder Cancer by siRNA Delivered with Exosomes
To use exosomes as a vector to deliver siRNA to silence the polo-like kinase 1 gene (PLK-1) in bladder cancer cells.
Source: Urology - February 11, 2016 Category: Urology & Nephrology Authors: Kristin A Greco, Carrie A. Franzen, Kimberly E. Foreman, Robert C. Flanigan, Paul C. Kuo, Gopal N. Gupta Tags: Basic and Translational Science Source Type: research

Small interfering RNA targeting heat shock protein 70 enhances chemosensitivity in human bladder cancer cells
Conclusions: Silencing of HSP70 expression using siRNA could be an attractive therapeutic strategy for bladder cancer by inducing inhibition of tumor growth as well as enhancing chemosensitivity.
Source: Urologic Oncology: Seminars and Original Investigations - September 5, 2011 Category: Urology & Nephrology Authors: Hosny M. Behnsawy, Hideaki Miyake, Yuji Kusuda, Masato Fujisawa Tags: Clinical - Bladder Source Type: research

Silencing of p53 RNA through transarterial delivery ameliorates renal tubular injury and down-regulates GSK-3β expression after ischemia-reperfusion injury.
Abstract p53, a pivotal protein in the apoptotic pathway, has been identified as a mediator of transcriptional responses to ischemia-reperfusion (IR) injury. The characteristics and functional significance of p53 response in vivo are largely unknown in IR-induced kidney injury. Therapeutic opportunities of delivering small interfering RNA (siRNA) via venous injection have gained recognition; however, systemic adverse effects of siRNA therapy should be considered. To prevent IR-induced kidney injury, we tested the efficacy of transarterial administration of siRNA targeting p53 (p53 siRNA). Female C57BL/6 mice under...
Source: Am J Physiol Renal P... - October 9, 2013 Category: Urology & Nephrology Authors: Fujino T, Muhib S, Sato N, Hasebe N Tags: Am J Physiol Renal Physiol Source Type: research

Silencing of p53 RNA through transarterial delivery ameliorates renal tubular injury and downregulates GSK-3{beta} expression after ischemia-reperfusion injury
p53, a pivotal protein in the apoptotic pathway, has been identified as a mediator of transcriptional responses to ischemia-reperfusion (IR) injury. The characteristics and functional significance of the p53 response in vivo are largely unknown in IR-induced kidney injury. Therapeutic opportunities of delivering small interfering RNA (siRNA) via venous injection have gained recognition; however, systemic adverse effects of siRNA therapy should be considered. To prevent IR-induced kidney injury, we tested the efficacy of transarterial administration of siRNA targeting p53 (p53 siRNA). Female C57BL/6 mice underwent unilatera...
Source: AJP: Renal Physiology - December 1, 2013 Category: Urology & Nephrology Authors: Fujino, T., Muhib, S., Sato, N., Hasebe, N. Tags: ARTICLES Source Type: research

Preliminary experimental study of urethral reconstruction with tissue engineering and RNA interference techniques.
This study investigated the feasibility of replacing urinary epithelial cells with oral keratinocytes and transforming growth factor-β1 (TGF-β1) small interfering RNA (siRNA)-transfected fibroblasts seeded on bladder acellular matrix graft (BAMG) in order to reconstruct tissue-engineered urethra. Constructed siRNAs, which expressed plasmids targeting TGF-β1, were transfected into rabbit fibroblasts. The effective siRNA was screened out by RT-PCR and was transfected into rabbit fibroblasts again. Synthesis of type I collagen in culture medium was measured by enzyme-linked immuno sorbent assay (ELISA). Autologous oral ker...
Source: Asian Journal of Andrology - May 1, 2013 Category: Urology & Nephrology Authors: Li C, Xu YM, Li HB Tags: Asian J Androl Source Type: research

Intrarenal ghrelin receptor inhibition ameliorates angiotensin ii-dependent hypertension in rats.
INTRARENAL GHRELIN RECEPTOR INHIBITION AMELIORATES ANGIOTENSIN II-DEPENDENT HYPERTENSION IN RATS. Am J Physiol Renal Physiol. 2018 Jun 20;: Authors: Kemp BA, Howell NL, Padia SH Abstract The intrarenal ghrelin receptor (GR) is localized to collecting duct (CD) cells where it increases αENaC-dependent sodium reabsorption in rodents. We hypothesized that chronic GR inhibition with intrarenal GR siRNA lowers blood pressure (BP) in Angiotensin II-dependent hypertension via reductions in αENaC-dependent sodium reabsorption. Uninephrectomized Sprague-Dawley rats (N=121) received subcutaneous osmotic pumps...
Source: Am J Physiol Renal P... - June 20, 2018 Category: Urology & Nephrology Authors: Kemp BA, Howell NL, Padia SH Tags: Am J Physiol Renal Physiol Source Type: research

Small interference RNA-mediated silencing of prostate stem cell antigen attenuates growth, reduces migration and invasion of human prostate cancer PC-3M cells
Conclusions: The present study provides the first evidence that silencing PSCA using siRNA can inhibit the proliferation and invasiveness properties of human CaP cells, which may provide a promising therapeutic strategy for CaP and open a novel avenue toward the investigation of the role of PSCA overexpression in cancers.
Source: Urologic Oncology: Seminars and Original Investigations - March 24, 2011 Category: Urology & Nephrology Authors: Zhigang Zhao, Wenjing Ma, Guohua Zeng, Defeng Qi, Lili Ou, Yeping Liang Tags: Clinical - Prostate Source Type: research

Low androgen status inhibits erectile function by up ‐regulating the expression of proteins of mitochondria‐associated membranes in rat corpus cavernosum
ConclusionLow androgen status up-regulated the expressions of patients in MAMs (FACL-4, PACS-2 and IP3R1) in the corpus cavernosum  and inhibited the eNOS/NO/cGMP signaling pathway, resulting in impaired erectile function in rats. Erectile function may be improved by inhibiting the high expression of PACS-2 in the corpus cavernosum under low androgen state.This article is protected by copyright. All rights reserved
Source: Andrology - April 25, 2022 Category: Urology & Nephrology Authors: Hao ‐Zhou Yang, Wen‐ju Xiong, Xiong Li, Jun Jiang, Rui Jiang Tags: ORIGINAL ARTICLE Source Type: research

Low androgen status inhibits erectile function by upregulating the expression of proteins of mitochondria ‐associated membranes in rat corpus cavernosum
ConclusionLow androgen status upregulated the expressions of patients in MAMs (FACL-4, PACS-2, and IP3R1) in the corpus cavernosum  and inhibited the eNOS/NO/cGMP signaling pathway, resulting in impaired erectile function in rats. Erectile function may be improved by inhibiting the high expression of PACS-2 in the corpus cavernosum under low androgen state.
Source: Andrology - May 2, 2022 Category: Urology & Nephrology Authors: Hao ‐Zhou Yang, Wen‐ju Xiong, Xiong Li, Jun Jiang, Rui Jiang Tags: ORIGINAL ARTICLE Source Type: research

Urethral Reconstruction With Tissue Engineering and RNA Interference Techniques in Rabbits
Conclusion: Our study suggested that oral keratinocytes and TGF-β1 siRNA transfected fibroblasts could be used as a source of seed cells for urethral tissue engineering.
Source: Urology - March 13, 2013 Category: Urology & Nephrology Authors: Chao Li, Yue-Min Xu, Zhang-Shun Liu, Hong-Bin Li Tags: Reconstructive Urology Source Type: research