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Specialty: Urology & Nephrology

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Total 430 results found since Jan 2013.

Inhibition of histone deacetylase 2 mitigates profibrotic TGF-β1 responses in fibroblasts derived from Peyronie's plaque.
This study was undertaken to determine the anti-fibrotic effect of small interfering RNA (siRNA)-mediated silencing of histone deacetylase 2 (HDAC2) in primary fibroblasts derived from human PD plaque. PD fibroblasts were pre-treated with HDAC2 siRNA and then stimulated with transforming growth factor-β1 (TGF-β1). Protein was extracted from treated fibroblasts for Western blotting and the membranes were probed with antibody to phospho-Smad2/Smad2, phospho-Smad3/Smad3, smooth muscle α-actin and extracellular matrix proteins, including plasminogen activator inhibitor-1, fibronectin, collagen I and collagen IV. We also per...
Source: Asian Journal of Andrology - June 17, 2013 Category: Urology & Nephrology Authors: Ryu JK, Kim WJ, Choi MJ, Park JM, Song KM, Kwon MH, Das ND, Kwon KD, Batbold D, Yin GN, Suh JK Tags: Asian J Androl Source Type: research

Long Noncoding RNA MALAT-1 is a New Potential Therapeutic Target for Castration Resistant Prostate Cancer
Conclusions: MALAT-1 may be needed to maintain prostate tumorigenicity and it is involved in prostate cancer progression. Thus, MALAT-1 may serve as a potential therapeutic target for castration resistant prostate cancer.
Source: The Journal of Urology - July 9, 2013 Category: Urology & Nephrology Authors: Shancheng Ren, Yawei Liu, Weidong Xu, Yi Sun, Ji Lu, Fubo Wang, Min Wei, Jian Shen, Jianguo Hou, Xu Gao, Chuanliang Xu, Jiaoti Huang, Yi Zhao, Yinghao Sun Tags: Investigative Urology Source Type: research

Mechanisms of AT1a receptor-mediated uptake of angiotensin II by proximal tubule cells: a novel role of the multi-ligand endocytic receptor megalin.
Abstract The present study tested the hypothesis that the multi-ligand endocytic receptor megalin is partially involved in the uptake of angiotensin II (ANG II) and downstream signaling responses in mouse proximal tubule cells (mPCT) by interacting with AT1a receptors. mPCT cells of wild-type (WT) and AT1a receptor-deficient (AT1a-KO) mice were treated with vehicle, the AT1 receptor blocker losartan (10 µM), or a selective megalin siRNA for 48 h. The uptake of fluorescein (FITC)-labeled ANG II (10 nM, 37(o)C) and downstream signaling responses were analyzed by fluorescence imaging and western blot. AT1a receptors...
Source: Am J Physiol Renal P... - April 16, 2014 Category: Urology & Nephrology Authors: Li XC, Zhuo JL Tags: Am J Physiol Renal Physiol Source Type: research

Indoxyl Sulfate Induces IL-6 Expression in Vascular Endothelial and Smooth Muscle Cells through OAT3-Mediated Uptake and Activation of AhR/NF-κB Pathway
Conclusion: IS induces IL-6 expression in vascular endothelial and smooth muscle cells through OAT3/AhR/NF-κB pathway.Nephron Exp Nephrol
Source: Nephron Experimental Nephrology - November 7, 2014 Category: Urology & Nephrology Source Type: research

Indoxyl Sulfate Induces IL-6 Expression in Vascular Endothelial and Smooth Muscle Cells through OAT3-Mediated Uptake and Activation of AhR/NF-κB Pathway.
Conclusion: IS induces IL-6 expression in vascular endothelial and smooth muscle cells through OAT3/AhR/NF-κB pathway. © 2014 S. Karger AG, Basel. PMID: 25376195 [PubMed - as supplied by publisher]
Source: Nephron Experimental Nephrology - November 5, 2014 Category: Urology & Nephrology Authors: Adelibieke Y, Yisireyili M, Ng HY, Saito S, Nishijima F, Niwa T Tags: Nephron Exp Nephrol Source Type: research

Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway
ConclusionSimulated physiological stretch increases the expression of ECM proteins in HBSMCs, and integrin α4/αv-FAK-ERK1/2 signaling pathway partly modulates the mechano-transducing process.
Source: World Journal of Urology - December 23, 2016 Category: Urology & Nephrology Source Type: research

Indoxyl Sulfate Induces IL-6 Expression in Vascular Endothelial and Smooth Muscle Cells through OAT3-Mediated Uptake and Activation of AhR/NF- & #954;B Pathway
Conclusion: IS induces IL-6 expression in vascular endothelial and smooth muscle cells through OAT3/AhR/NF-#954;B pathway.Nephron Exp Nephrol 2014;128:1-8
Source: Nephron Experimental Nephrology - November 9, 2017 Category: Urology & Nephrology Source Type: research

The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor
Conclusions: Our study suggests reciprocal regulation of PlncRNA-1 and androgen receptor contribute to CaP pathogenesis and that PlncRNA-1 is a potential therapy target.
Source: Urologic Oncology: Seminars and Original Investigations - January 24, 2012 Category: Urology & Nephrology Authors: Zilian Cui, Shancheng Ren, Ji Lu, Fubo Wang, Weidong Xu, Yi Sun, Min Wei, Junyi Chen, Xu Gao, Chuanliang Xu, Jian-Hua Mao, Yinghao Sun Tags: Laboratory - Prostate Source Type: research

Mechanisms of AT1a receptor-mediated uptake of angiotensin II by proximal tubule cells: a novel role of the multiligand endocytic receptor megalin
The present study tested the hypothesis that the multiligand endocytic receptor megalin is partially involved in the uptake of ANG II and downstream signaling responses in mouse proximal tubule cells (mPCT) by interacting with AT1a receptors. mPCT cells of wild-type (WT) and AT1a receptor-deficient (AT1a-KO) mice were treated with vehicle, the AT1 receptor blocker losartan (10 μM), or a selective megalin small interfering (si) RNA for 48 h. The uptake of fluorescein (FITC)-labeled ANG II (10 nM, 37°C) and downstream signaling responses were analyzed by fluorescence imaging and Western blotting. AT1a receptors and me...
Source: AJP: Renal Physiology - July 15, 2014 Category: Urology & Nephrology Authors: Li, X. C., Zhuo, J. L. Tags: INNOVATIVE METHODOLOGY Source Type: research

Mechanisms of Bradykinin-Induced Expression of Connective Tissue Growth Factor and Nephrin in Podocytes.
Abstract Diabetic Nephropathy (DN) is the main cause of morbidity and mortality in diabetes, characterized by mesangial matrix deposition and podocytopathy including podocyte loss. The risk factors and mechanisms involved in the pathogenesis of DN are still not completely defined. In the current study we aim to understand the cellular mechanisms through which activation of B2 kinin receptors contribute to the initiation and progression of DN. Stimulation of cultured rat podocytes with bradykinin (BK) resulted in a significant increase in reactive oxygen species generation (ROS) and this was associated with a signi...
Source: Am J Physiol Renal P... - October 7, 2015 Category: Urology & Nephrology Authors: Abou Msallem JP, Chalhoub H, Al Hariri M, Saad L, Jaffa M, Ziyadeh F, Jaffa AA Tags: Am J Physiol Renal Physiol Source Type: research

The Vav3 oncogene enhances the malignant potential of prostate cancer cells under chronic hypoxia.
CONCLUSIONS: Our results demonstrate that Vav3 plays a crucial role in prostate cancer growth and malignant behavior, thus revealing a novel potential therapeutic target. PMID: 23403204 [PubMed - as supplied by publisher]
Source: Urologic Oncology - February 8, 2013 Category: Urology & Nephrology Authors: Hirai K, Nomura T, Yamasaki M, Inoue T, Narimatsu T, Chisato Nakada PD, Yoshiyuki Tsukamoto PD, Matsuura K, Sato F, Moriyama M, Mimata H Tags: Urol Oncol Source Type: research

The Role of c-FLIP in Cisplatin Resistance of Human Bladder Cancer Cells
Purpose: We investigated the mechanisms underlying cisplatin resistance in human bladder cancer cells to provide novel molecular targets for the treatment of cisplatin resistant bladder cancer. Materials and Methods: The differential gene expression of cisplatin sensitive (T24) and resistant (T24R2) human bladder cancer cell lines was analyzed and validated by microarray and Western blot analysis. Changes in cisplatin sensitivity by c-FLIP knockdown and related mechanisms in T24R2 cells were assessed using the Cell Counting Kit-8 assay (Dojindo Molecular Technologies, Gaithersburg, Maryland) and Western blot. siRNA olig...
Source: The Journal of Urology - January 10, 2013 Category: Urology & Nephrology Authors: Sangchul Lee, Cheol Yong Yoon, Seok-Soo Byun, Eunsik Lee, Sang Eun Lee Tags: Investigative Urology Source Type: research

p62/SQSTM1 is required for cell survival of apoptosis‐resistant bone metastatic prostate cancer cell lines
CONCLUSIONSA pattern emerges wherein the BMSC‐sensitive PCa cell lines are known to be osteoblastic and express the androgen receptor, while the BMSC‐insensitive PCa cell lines are characteristically osteolytic and do not express the androgen receptor. Furthermore, BMSC‐insensitive PCa may have evolved a dependency on p62 for cell survival that could be exploited to target and kill these apoptosis‐resistant PCa cells in the bone. Prostate © 2013 Wiley Periodicals, Inc.
Source: The Prostate - September 30, 2013 Category: Urology & Nephrology Authors: Megan A. Chang, Micaela Morgado, Curtis R. Warren, Cimona V. Hinton, Mary C. Farach‐Carson, Nikki A. Delk Tags: Original Article Source Type: research

This Month in Investigative Urology
MALAT-1, a long noncoding RNA, was described as a regulator of metastasis and motility, and is over expressed in various human solid tumors. In this issue of The Journal Ren et al (page 2278) from the People's Republic of China evaluated MALAT-1 expression in prostate cancer tissues and cell lines, and studied the therapeutic effects of MALAT-1 silencing on castration resistant prostate cancer (CRPC) cells in vitro and in vivo. Quantitative reverse transcriptase-polymerase chain reaction was done to detect MALAT-1 expression and examine the silencing effect of siRNA designed against MALAT-1. The biological effects of M...
Source: The Journal of Urology - September 16, 2013 Category: Urology & Nephrology Authors: Karl-Erik Andersson Source Type: research

Dual inhibition by S6K1 and Elf4E is essential for controlling cellular growth and invasion in bladder cancer☆,☆☆?>
CONCLUSION: These findings suggest that both the mTOR pathway downstream of eukaryotic initiation factor 4E and S6K1 can be successfully inhibited, therefore, the recurrence of bladder cancer can be prevented by high-dose rapamycin only, suggesting that 4E-BP1 might be still under mTORC1. PMID: 24239466 [PubMed - as supplied by publisher]
Source: Urologic Oncology - November 13, 2013 Category: Urology & Nephrology Authors: Kyou Kwon J, Kim SJ, Hoon Kim J, Mee Lee K, Ho Chang I Tags: Urol Oncol Source Type: research