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Specialty: Cytology
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Total 2613 results found since Jan 2013.
Mfsd2a attenuated hypoxic-ischemic brain damage via protection of the blood-brain barrier in mfat-1 transgenic mice
This study aims to investigate the mechanisms of the protective effect against hypoxic-ischemic brain damage (HIBD) of mfat-1 transgenic mice. mfat-1 mice not only demonstrated a significant amelioration of neurological dysfunction and neuronal damage but also partly maintained the physiological permeability of the BBB after HIBD. We initially showed this was associated with elevated major facilitator superfamily domain-containing 2a (Mfsd2a) expression on the BBB, resulting from more lysophosphatidylcholine (LPC)-DHA entering the brain. Wild-type (WT) mice showed a similar Mfsd2a expression trend after long-term feeding w...
Source: Cellular and Molecular Life Sciences : CMLS - February 23, 2023 Category: Cytology Authors: Xiaoxue Li Yumeng Zhang Jianghao Chang Chenglin Zhang Lin Li Yifan Dai Haiyuan Yang Ying Wang Source Type: research
G βγ signaling regulates microtubule-dependent control of Golgi integrity
In this study, we use siRNA against Gβ1/2 or specific Gγ subunits to deplete their expression, and show that their knockdown causes a significant reduction in nocodazole-induced Golgi fragmentation. We establish that knockdown of Gβγ or inhibition of Gβγ with gallein resulted in decreased activation of protein kinase D (PKD) in response to nocodazole treatment. We demonstrate that restricting the amount of free Gβγ available for signaling by either inhibiting Gαi activation using pertussis toxin or by knockdown of the non-GPCR GEF, Girdin/GIV protein, results in a substantial decrease in nocodazole-induced Golgi f...
Source: Cellular Signalling - February 22, 2023 Category: Cytology Authors: Kalpana Rajanala Philip B Wedegaertner Source Type: research
SPOP is essential for DNA replication licensing through maintaining translation of CDT1 and CDC6 in HaCaT cells
In this study, we evaluated the cellular functions of wild-type SPOP in non-cancerous human keratinocyte-derived HaCaT cells expressing wild-type SPOP gene. SPOP knockdown using siRNA in HaCaT cells dramatically reduced cell growth and arrested their cell cycles at G1/S phase. The expression of DNA replication licensing factors CDT1 and CDC6 in HaCaT cells drastically decreased on SPOP knockdown as their translation was inhibited. CDT1 and CDC6 downregulation induced p21 expression without p53 activation. Our results suggest that SPOP is essential for DNA replication licensing in non-cancerous keratinocyte HaCaT cells.PMID...
Source: Cell Research - February 15, 2023 Category: Cytology Authors: Sayoko Sanada Masashi Maekawa Sota Tate Hiroki Nakaoka Yasuhiro Fujisawa Koji Sayama Shigeki Higashiyama Source Type: research
Carnosol inhibits KGN cells oxidative stress and apoptosis and attenuates polycystic ovary syndrome phenotypes in mice through Keap1-mediated Nrf2/HO-1 activation
In this study, we established hyperandrogenic PCOS models both in vitro and in vivo. In the human ovarian granulosa cell line, KGN cells, decreased viability and mitochondrial membrane potential, and upregulated reactive oxygen species (ROS) level and apoptosis induced by DHT were partly reversed by carnosol. Western blotting results showed that carnosol treatment inhibited the DHT-activated mitochondrial apoptotic pathway by activating nuclear factor-erythroid 2-related factor (Nrf2)/heme oxygenase 1 (HO-1). Knockdown of Nrf2 by transfecting with siRNA or inhibiting HO-1 by zinc protoporphyrin (ZnPP) blocked the protectiv...
Source: Cell Research - February 14, 2023 Category: Cytology Authors: Rui Ji Fangyuan Jia Xin Chen Yue Gao Jing Yang Source Type: research
Iroquois homeobox 3 regulates odontoblast proliferation and differentiation mediated by Wnt5a expression
Biochem Biophys Res Commun. 2023 Feb 4;650:47-54. doi: 10.1016/j.bbrc.2023.02.004. Online ahead of print.ABSTRACTIroquois homeobox (Irx) genes are TALE-class homeobox genes that are evolutionarily conserved across species and have multiple critical cellular functions in fundamental tissue development processes. Previous studies have shown that Irxs genes are expressed during tooth development. However, the precise roles of genes in teeth remain unclear. Here, we demonstrated for the first time that Irx3 is an essential molecule for the proliferation and differentiation of odontoblasts. Using cDNA synthesized from postnatal...
Source: Cell Research - February 11, 2023 Category: Cytology Authors: Anrizandy Narwidina Aya Miyazaki Kokoro Iwata Rika Kurogoushi Asuna Sugimoto Yasusei Kudo Keita Kawarabayashi Yoshihito Yamakawa Yuki Akazawa Takamasa Kitamura Hiroshi Nakagawa Kimiko Yamaguchi-Ueda Tomokazu Hasegawa Keigo Yoshizaki Satoshi Fukumoto Akihi Source Type: research
Effects of Semaphorin3A on the growth of sensory and motor neurons
Exp Cell Res. 2023 Feb 8:113506. doi: 10.1016/j.yexcr.2023.113506. Online ahead of print.ABSTRACTAfter peripheral nerve injury, motor and sensory axons can regenerate, but the inaccurate reinnervation of the target leads to poor functional recovery. Schwann cells (SCs) express sensory and motor phenotypes associated with selective regeneration. Semaphorin 3A (Sema3A) is an axonal chemorepellent that plays an essential role in axon growth. SCs can secret Sema3A, and Sema3A presents a different expression pattern at the proximal and distal ends of injured sensory and motor nerves. Hence, in our study, the protein expression ...
Source: Experimental Cell Research - February 10, 2023 Category: Cytology Authors: Mi Shen Chun Zhou Yingchao Tian Tongxin Shang None Qingyun Liang Mengru Ming Fei Ding Yuhua Ji Source Type: research
MDM2 promotes cancer cell survival through regulating the expression of HIF-1 α and pVHL in retinoblastoma
In this study, we investigated the role of MDM2 and its relation to HIF-1α in RB. Expression analysis on primary human RB samples showed that MDM2 expression was positively correlated with that of HIF-1α while negatively correlated with von Hippel-Lindau protein (pVHL), the regulator of HIF-1α. In agreement, RB cells with MDM2 overexpression showed increased expression of HIF-1α and decreased expression of pVHL, while cells with MDM2 siRNA knockdown or MDM2-specific inhibitor showed the opposite effect under hypoxia. Further immuno-precipitation analysis revealed that MDM2 could directly interact with pVHL and promotes...
Source: Cell Research - February 6, 2023 Category: Cytology Authors: Shouhua Zhang Hongyan Xu Weiming Li Jianfeng Ji Qifang Jin Leifeng Chen Qiang Gan Qiang Tao Yong Chai Source Type: research
Recombinant Adenovirus siRNA Knocking Down the Ndufs4 Gene Alleviates Myocardial Apoptosis Induced by Oxidative Stress Injury
In conclusion, we successfully constructed Ndufs4 siRNA recombinant adenovirus; furthermore, the downexpression of the Ndufs4 gene may alleviate H2O2-induced H9c2 cell apoptosis.PMID:36741296 | PMC:PMC9897913 | DOI:10.1155/2023/8141129
Source: Cell Research - February 6, 2023 Category: Cytology Authors: Beibei Wang Jinsheng Zhang Aijun Xu Source Type: research
Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma
CONCLUSIONS: PAPSS1 may be an ESCC suppressor gene, and its specific molecular mechanism in ESCC needs to be further studied.PMID:36734141 | DOI:10.14670/HH-18-590
Source: Histology and Histopathology - February 3, 2023 Category: Cytology Authors: Xuyou Zhu Peipei Zhu Xue Chen Long Zhang Caixia Wu Haoyang Zhang Xiaoying Shen Yao Qi Mingmin Chen Shunli Wang Xianghua Yi Source Type: research
Screening of an epigenetic compound library identifies BRD4 as a potential antiviral target for hepatitis B virus covalently closed circular DNA transcription
In this study, we screened an epigenetic compound library in the cccDNA reporter cell line HepBHAe82, which produces the HA-tagged HBeAg in a cccDNA-dependent manner. Among the obtained hits, a bromodomain-containing protein 4 (BRD4) inhibitor MS436 exhibited marked inhibition of cccDNA transcription in both HBV stable cell line HepAD38 and HepG2-NTCP or primary human hepatocyte infection system under noncytotoxic concentrations. Chromatin immunoprecipitation (ChIP) assay demonstrated that MS436 dramatically reduced the enrichment of H3K27ac, an activating histone modification pattern, on cccDNA minichromosome. RNAseq diff...
Source: Cell Research - February 3, 2023 Category: Cytology Authors: Xiaoyang Yu Quanxin Long Sheng Shen Zhentao Liu Jithin Chandran Junjie Zhang Hao Ding Hu Zhang Dawei Cai Elena S Kim Yufei Huang Haitao Guo Source Type: research
Lipid Nanoparticles for Nucleic Acid Delivery to Endothelial Cells
Pharm Res. 2023 Feb 3. doi: 10.1007/s11095-023-03471-7. Online ahead of print.ABSTRACTEndothelial cells play critical roles in circulatory homeostasis and are also the gateway to the major organs of the body. Dysfunction, injury, and gene expression profiles of these cells can cause, or are caused by, prevalent chronic diseases such as diabetes, cardiovascular disease, and cancer. Modulation of gene expression within endothelial cells could therefore be therapeutically strategic in treating longstanding disease challenges. Lipid nanoparticles (LNP) have emerged as potent, scalable, and tunable carrier systems for deliverin...
Source: Cell Research - February 3, 2023 Category: Cytology Authors: Gary W Liu Edward B Guzman Nandita Menon Robert S Langer Source Type: research
Correlation of 3'-Phosphoadenosine-5'-Phosphosulfate synthase 1 (PAPSS1) expression with clinical parameters and prognosis in esophageal squamous carcinoma
CONCLUSIONS: PAPSS1 may be an ESCC suppressor gene, and its specific molecular mechanism in ESCC needs to be further studied.PMID:36734141 | DOI:10.14670/HH-18-590
Source: Histology and Histopathology - February 3, 2023 Category: Cytology Authors: Xuyou Zhu Peipei Zhu Xue Chen Long Zhang Caixia Wu Haoyang Zhang Xiaoying Shen Yao Qi Mingmin Chen Shunli Wang Xianghua Yi Source Type: research
