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Specialty: Cytology
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Total 2613 results found since Jan 2013.
Downregulation of < em > CLDN6 < /em > inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma
CONCLUSIONS: CLDN6 knockdown results in the inhibition of HCC cells' infiltration and migration and promotes apoptosis via downregulation of the JAK2/STAT3 signaling pathway.PMID:37588735 | PMC:PMC10425638 | DOI:10.21037/tcr-23-19
Source: Cell Research - August 17, 2023 Category: Cytology Authors: Zhiyi He Fangtian Fan Zhidong Xu Mei Zhang Rui Zhao Xiquan Ke Qizhi Wang Shanjun Yan Hailun Zheng Source Type: research
Purinergic 2X 4 (P2X4), but not P2X7, receptors increase cytosolic Ca < sup > 2+ < /sup > and stimulate mucin secretion in rat conjunctival goblet cells to maintain ocular surface health
Exp Eye Res. 2023 Aug 12:109614. doi: 10.1016/j.exer.2023.109614. Online ahead of print.ABSTRACTIonotropic purinergic receptors (P2XRs) are activated by ATP and ATP analogs. ATP can be released through ATP-permeable channels such as the pannexin hemichannels. Upon activation, the P2XRs become permeable to Ca2+, a potent stimulator of mucin secretion in conjunctival goblet cells (CGCs). The purpose of this study was to investigate the presence and function of P2XRs in CGCs. We also examined the presence of pannexin hemichannels. Rat first passage CGCs were stained with the goblet cell marker anti-cytokeratin 7 antibody and ...
Source: Cell Research - August 14, 2023 Category: Cytology Authors: Haakon K Fj ærvoll Ketil A Fj ærvoll Menglu Yang Jeffrey Bair Tor P Utheim Darlene A Dartt Source Type: research
The formin DAAM1 regulates the deubiquitinase activity of USP10 and integrin homeostasis
In this study, a yeast two-hybrid screen revealed a novel binding partner for USP10, the formin, DAAM1. We found that DAAM1 binds to and inhibits USP10's DUB activity through the FH2 domain of DAAM1 independent of its actin functions. The USP10/DAAM1 interaction was also supported by proximity ligation assay (PLA) in primary human corneal fibroblasts. Treatment with TGFβ1 significantly increased USP10 and DAAM1 protein expression, PLA signal, and co-localization to actin stress fibers. DAAM1 siRNA knockdown significantly reduced co-precipitation of USP10 and DAAM1 on purified actin stress fibers, and β1- and β5-integrin...
Source: European Journal of Cell Biology - August 10, 2023 Category: Cytology Authors: Andrew T Phillips Edward F Boumil Arunkumar Venkatesan Christine Tilstra-Smith Nileyma Castro Barry E Knox Jessica L Henty-Ridilla Audrey M Bernstein Source Type: research
miR-147b is an oncomiR acting synergistically with HIPK2 to promote pancreatic carcinogenesis
This study addressed this issue based on in silico prediction, in vitro experiments, and in vivo validation. The differentially expressed PCa-related miRNAs and bioinformatics tools predicted downstream regulators. The expression of miR-147b was examined in PCa cell lines. Putative targets of miR-147b were predicted by a publicly available database and confirmed by luciferase activity assay. Mimic/inhibitor, siRNA/overexpression plasmid, or pifithrin-α (p53 inhibitor) were delivered into PCa cells to assess the effect of miR-147b, HIPK2, and p53 on malignant phenotypes of PCa cells. AntagomiR-147b and shRNA targeting HIPK...
Source: Cellular Signalling - August 5, 2023 Category: Cytology Authors: Ganggang Wang Zenghui Ma Chao Song Xiaoliang Wang Zhijie Zhou Source Type: research
3-deazaneplanocin A, a histone methyltransferase inhibitor, improved the chemoresistance induced under hypoxia in melanoma cells
In conclusion, a histone methyltransferase EZH2 inhibitor, DZNep was capable of tackling acquired chemoresistance via the suppression of histone methylation induced under hypoxic tumor microenvironment.PMID:37542772 | DOI:10.1016/j.bbrc.2023.08.003
Source: Cell Research - August 5, 2023 Category: Cytology Authors: Mika Hosokawa Sekai Tetsumoto Mirano Yasui Yusuke Kono Ken-Ichi Ogawara Source Type: research
HIF-1 α is positively associated with endometrial receptivity by regulating PKM2
CONCLUSIONS: HIF-1α plays a crucial role in the building of ER through regulating glycolysis.PMID:37533344 | DOI:10.1111/jog.15752
Source: Cell Research - August 3, 2023 Category: Cytology Authors: Lixia Liang Yang Yang Lin Yang Xiuping Zhang Suming Xu Yanling Liu Xueqing Wu Lan Chao Source Type: research
PGC-1 α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1
We examined the expression of PGC-1α after sciatic nerve crush or transection using Western blotting and found that PGC-1α expression increased after PNI. Then we utilized ex vivo and in vitro models to investigate the effects of PGC-1α inhibition and activation on Schwann cell dedifferentiation and nerve degeneration. Our findings indicate that PGC-1α negatively regulates Schwann cell dedifferentiation and nerve degeneration. Through the use of RNA-seq, siRNA/plasmid transfection and reversal experiments, we identified that PGC-1α targets inhibit the expression of paraoxonase 1 (PON1) during Schwann cell dedifferenti...
Source: Cellular and Molecular Neurobiology - August 1, 2023 Category: Cytology Authors: Ying Zou Shu Wu Fei Wen Yuanlong Ge Shengkang Luo Source Type: research
TNFAIP2 confers cisplatin resistance in head and neck squamous cell carcinoma via KEAP1/NRF2 signaling
CONCLUSIONS: Our results reveal the antioxidant and cisplatin resistance-regulating roles of the TNFAIP2/KEAP1/NRF2/JNK axis in HNSCC, suggesting that TNFAIP2 might be a potential target in improving the cisplatin treatment effect, particularly for patients with cisplatin resistance.PMID:37525222 | PMC:PMC10391982 | DOI:10.1186/s13046-023-02775-1
Source: Cell Research - July 31, 2023 Category: Cytology Authors: Teng Xu Yuemei Yang Zhihong Chen Jinsong Wang Xiaolei Wang Yang Zheng Chao Wang Yachen Wang Zaiou Zhu Xu Ding Junbo Zhou Gang Li Hongchuang Zhang Wei Zhang Yunong Wu Xiaomeng Song Source Type: research
SGLT2 inhibitor canagliflozin alleviates vascular calcification through suppression of NLRP3 inflammasome
CONCLUSIONS: Our study for the first time demonstrates that CANA exerts a protective effect on vascular calcification at least partially via suppressing NLRP3 signaling pathway. Therefore, supplementation of CANA as well as inhibition of NLRP3 inflammasome presents a potential therapy for vascular calcification.PMID:37523743 | DOI:10.1093/cvr/cvad119
Source: Cell Research - July 31, 2023 Category: Cytology Authors: An Chen Zirong Lan Li Li Luting Xie Xiaoyu Liu Xiulin Yang Siyi Wang Qingchun Liang Qianqian Dong Liyun Feng Yining Li Yuanzhi Ye Mingwei Fu Lihe Lu Jianyun Yan Source Type: research
Stable expression of shRNA for the control of recombinant adenovirus replication
Braz J Med Biol Res. 2023 Jul 21;56:e12682. doi: 10.1590/1414-431X2023e12682. eCollection 2023.ABSTRACTPreventing the replication of adenovirus could have practical uses, such as controlling infection with wild-type virus or in applications involving recombinant vectors. Mainly transient methods have been used to inhibit adenovirus replication, including siRNA or drugs. Here, we tested whether stable expression of shRNA designed to target hexon, Iva2, or pol can inhibit the replication of a recombinant adenoviral vector, Ad-LacZ (serotype 5, E1/E3 deleted), in 293T cells. Significant knockdown correlating with reduced Ad-L...
Source: Cell Research - July 26, 2023 Category: Cytology Authors: M V G Lana F Antunes N G Tessarollo B E Strauss Source Type: research
MicroRNA-140 is not involved in sepsis-induced muscle atrophy
Am J Physiol Cell Physiol. 2023 Jul 24. doi: 10.1152/ajpcell.00157.2023. Online ahead of print.ABSTRACTSepsis is a life-threatening inflammatory response to infection, often accompanied by skeletal muscle atrophy. A previous study demonstrated that the administration of microRNA-140 (miR-140) attenuated lipopolysaccharides (LPS)-induced muscle atrophy, whereas miR-140 knockdown with siRNA promoted atrophy. Therefore, we investigated whether miR-140 is involved in LPS-induced muscle atrophy using a genetic model, miR-140-/- mice. We found that a single injection of LPS induced atrophy both in slow-twitch and fast-twitch mus...
Source: Am J Physiol Cell Ph... - July 24, 2023 Category: Cytology Authors: Jaehoon Shin Shigeru Miyaki Hiroshi Asahara Takayuki Akimoto Source Type: research
