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Specialty: Cytology
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Total 2613 results found since Jan 2013.
Linc-ROR Regulates < em > POU5F1 < /em > and < em > SOX2 < /em > by Competitively Binding miR-145-5p to Affect the Proliferation and Migration of Gastric Cancer Cells
In this study, in situ hybridization revealed that long intergenic non-protein coding RNA-regulator of reprogramming (linc-ROR) was highly expressed in gastric cancer cells and was mainly cytoplasmic-positive. Cell counting kit-8 (CCK-8), plate colony formation, wound healing, and Transwell assay revealed that linc-ROR knockdown impedes the growth, proliferation, and migration of gastric cancer cells, while linc-ROR overexpression promoted gastric cancer cell growth, migration, and colony formation ability. Combined with previous studies, the molecular mechanism axis of linc-ROR/miR-145-5-5p/POU5F1/SOX2 was verified. The e...
Source: Cell Transplantation - June 12, 2023 Category: Cytology Authors: Xiuchong Yu Xinyi Ma Yangtao Jia Xinxin Wu Zhilong Yan Source Type: research
siRNA screening reveals that SNAP29 contributes to exosome release
Cell Mol Life Sci. 2023 Jun 7;80(7):177. doi: 10.1007/s00018-023-04822-8.ABSTRACTCells release extracellular vesicles (EVs) of different sizes. Small EVs (< 200 nm) can originate from the fusion of multivesicular bodies with the plasma membrane, i.e. exosomes, and from budding of the plasma membrane, i.e. small ectosomes. To investigate the molecular machinery required for the release of small EVs, we developed a sensitive assay based on incorporation of radioactive cholesterol in EV membranes and used it in a siRNA screening. The screening showed that depletion of several SNARE proteins affected the release of small EV...
Source: Cellular and Molecular Life Sciences : CMLS - June 7, 2023 Category: Cytology Authors: Nina Pettersen Hessvik Krizia Sagini Silvana Romero Manuel Ramirez-Garrastacho Marta Rodriguez Astrid Elisabeth V Tutturen Audun Kvalvaag Espen Stang Andreas Brech Kirsten Sandvig Alicia Llorente Source Type: research
Matrix metalloproteinase-2 delivery to extracellular vesicles produced by HPV-positive cervical cancer cells enhances metastasis via the Akt pathway
Cell Biol Int. 2023 Jun 5. doi: 10.1002/cbin.12054. Online ahead of print.ABSTRACTHere, we aimed to analyze the effects of matrix metalloproteinase-2 (MMP-2) delivery to extracellular vesicles (EVs) secreted by human papillomavirus (HPV)-associated cervical cancer cells on human umbilical vein endothelial cell (HUVEC) angiogenesis. First, MMP-2 expression was compared among SiHa (HPV16), HeLa (HPV18), and C-33A (negative) cells. Then, EVs were isolated from these cells, and MMP-2 expression in the EVs was compared. SiHa and HeLa cells were transfected with MMP-2 or control siRNA. HUVECs were treated with EVs isolating from...
Source: Cell Biology International - June 5, 2023 Category: Cytology Authors: Jianan Sun Junjun Mou Yunmei Zhang Kang Yuan Ying Yang Qing Tang Source Type: research
TM6SF2 reduces lipid accumulation in vascular smooth muscle cells by inhibiting LOX-1 and CD36 expression
Exp Cell Res. 2023 Jun 2:113666. doi: 10.1016/j.yexcr.2023.113666. Online ahead of print.ABSTRACTTM6SF2, predominantly expressed in the liver and intestine, is closely associated with lipid metabolism. We have demonstrated the presence of TM6SF2 in VSMCs within human atherosclerotic plaques. Subsequent functional studies were conducted to investigate its role in lipid uptake and accumulation in human vascular smooth muscle cells (HAVSMCs) using siRNA knockdown and overexpression techniques. Our results showed that TM6SF2 reduced lipid accumulation in oxLDL-stimulated VSMCs, likely through the regulation of lectin-like oxLD...
Source: Experimental Cell Research - June 4, 2023 Category: Cytology Authors: Ting-Ting Li Yu-Ting Cui Tao-Hua Li Qiong Xiang Yan-Yu Chen Xi-Long Zheng Juan Peng Zhi-Han Tang Source Type: research
VCAN activates JAK/STAT signaling pathway to promote the progression of LPS-induced acute pneumonia
ConclusionsVCAN knockdown alleviates lung injury of mice with pneumonia by inhibiting the JAK/STAT signaling pathway.
Source: Molecular and Cellular Toxicology - June 3, 2023 Category: Cytology Source Type: research
