Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis
Histol Histopathol. 2021 Mar 4:18324. doi: 10.14670/HH-18-324. Online ahead of print.ABSTRACTBACKGROUND: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness.OBJECTIVES: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis.MATERIALS AND METHODS: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples w...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Emilio Lopez-Trujillo M ònica Gonzàlez-Farré Ramon M Pujol Beatriz Bellosillo Roser Fisa Cristina Riera Magdalena Alcover Carlos Barranco Gemma Martin-Ezquerra Source Type: research

Overexpression of hsa_circ_0094742 inhibits IL-1 β-induced decline in CHON-001 cell viability by targeting microRNA-127-5p
Histol Histopathol. 2021 Mar 5:18325. doi: 10.14670/HH-18-325. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a public health problem that affects 240 million people globally; however, the current treatment options for OA are not effective. Therefore, there is still an urgent need to identify novel strategies to reduce the incidence and progression of OA. The circular RNA hsa_circ_0094742 was reported to be downregulated in patients with OA. However, the underlying mechanism remains unclear. The levels of hsa_circ_0094742 in CHON-001 were detected by reverse transcription quantitative polymerase chain reaction. Moreo...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Mingqi Sun Junli Yang Dianming Jiang Guoyu Bao Source Type: research

Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis
Histol Histopathol. 2021 Mar 4:18324. doi: 10.14670/HH-18-324. Online ahead of print.ABSTRACTBACKGROUND: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness.OBJECTIVES: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis.MATERIALS AND METHODS: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples w...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Emilio Lopez-Trujillo M ònica Gonzàlez-Farré Ramon M Pujol Beatriz Bellosillo Roser Fisa Cristina Riera Magdalena Alcover Carlos Barranco Gemma Martin-Ezquerra Source Type: research

Overexpression of hsa_circ_0094742 inhibits IL-1 β-induced decline in CHON-001 cell viability by targeting microRNA-127-5p
Histol Histopathol. 2021 Mar 5:18325. doi: 10.14670/HH-18-325. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a public health problem that affects 240 million people globally; however, the current treatment options for OA are not effective. Therefore, there is still an urgent need to identify novel strategies to reduce the incidence and progression of OA. The circular RNA hsa_circ_0094742 was reported to be downregulated in patients with OA. However, the underlying mechanism remains unclear. The levels of hsa_circ_0094742 in CHON-001 were detected by reverse transcription quantitative polymerase chain reaction. Moreo...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Mingqi Sun Junli Yang Dianming Jiang Guoyu Bao Source Type: research

Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal anti-leishmania antibodies in cutaneous leishmaniasis
Histol Histopathol. 2021 Mar 4:18324. doi: 10.14670/HH-18-324. Online ahead of print.ABSTRACTBACKGROUND: Different immunohistochemical markers to detect amastigotes in cutaneous leishmaniasis have been proposed with variable diagnostic usefulness.OBJECTIVES: To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous leishmaniasis.MATERIALS AND METHODS: Thirty-three skin samples corresponding to PCR confirmed cutaneous leishmaniasis were included in the study. All samples w...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Emilio Lopez-Trujillo M ònica Gonzàlez-Farré Ramon M Pujol Beatriz Bellosillo Roser Fisa Cristina Riera Magdalena Alcover Carlos Barranco Gemma Martin-Ezquerra Source Type: research

Overexpression of hsa_circ_0094742 inhibits IL-1 β-induced decline in CHON-001 cell viability by targeting microRNA-127-5p
Histol Histopathol. 2021 Mar 5:18325. doi: 10.14670/HH-18-325. Online ahead of print.ABSTRACTOsteoarthritis (OA) is a public health problem that affects 240 million people globally; however, the current treatment options for OA are not effective. Therefore, there is still an urgent need to identify novel strategies to reduce the incidence and progression of OA. The circular RNA hsa_circ_0094742 was reported to be downregulated in patients with OA. However, the underlying mechanism remains unclear. The levels of hsa_circ_0094742 in CHON-001 were detected by reverse transcription quantitative polymerase chain reaction. Moreo...
Source: Histology and Histopathology - March 5, 2021 Category: Cytology Authors: Mingqi Sun Junli Yang Dianming Jiang Guoyu Bao Source Type: research

The function and regulation mechanism of piRNAs in human cancers
Histol Histopathol. 2021 Mar 2:18323. doi: 10.14670/HH-18-323. Online ahead of print.ABSTRACTPiwi-interacting RNAs (piRNAs) are mainly expressed in mammalian germ cells, playing an important role in maintaining germ line DNA integrity, inhibiting transposon transcription and translation, participating in heterochromatin formation, epigenetic regulation, and germ cell genesis. They combine with P-element induced wimpy testis (PIWI) proteins to form effector complexes known as piRNA-induced silencing complexes (pi-RISC) to regulate the gene silencing pathway. Recent evidence suggests that numerous piRNAs, with tumor-promotin...
Source: Histology and Histopathology - March 2, 2021 Category: Cytology Authors: Wu Wu Bing-Feng Lu Ru-Qi Jiang Shuo Chen Source Type: research

Defective expression of the peroxisome regulators PPAR α receptors and lysogenesis with increased cellular senescence in the venous wall of chronic venous disorder
Histol Histopathol. 2021 Mar 1:18322. doi: 10.14670/HH-18-322. Online ahead of print.ABSTRACTThe pathogenesis of chronic venous disorder (CVeD) remains partially understood. A marked wall remodeling has been shown with potential accelerated tissue senescence. We have investigated the expression of peroxisome proliferator-activated receptor (PPAR) isoforms transcription factor EB (TFEB) as regulatory molecules of cellular homeostasis and makers of peroxisomal and lysosomal biogenesis. We have also quantified p16 expression as a cellular senescence marker. In specimens of maior safena vein from 35 CVeD and 27 healthy venous ...
Source: Histology and Histopathology - March 1, 2021 Category: Cytology Authors: Miguel A Ortega Oscar Fraile-Mart ínez Leonel Pekarek Miguel A Alvarez-Mon Ángel Asúnsolo Lara Sanchez-Trujillo Santiago Coca Julia Buj án Melchor Álvarez-Mon Natalio Garc ía-Honduvilla Felipe Sainz Source Type: research

The long-term effects of rapamycin-based immunosuppressive protocols on the expression of renal aquaporins 1, 2, 3 and 4 water channels in rats
CONCLUSIONS: The long-term immunosuppressive treatment using multi-drug protocols decreased AQP1-4 expressions in renal tubules, possibly leading to impaired urine-concentrating ability in rat.PMID:33634832 | DOI:10.14670/HH-18-321 (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 26, 2021 Category: Cytology Authors: Marta Grabowska Katarzyna Micha łek Karolina K ędzierska-Kapuza Andrzej Kram Kamil Gill Ma łgorzata Piasecka Source Type: research

Expression of Claudin-1 in laryngeal squamous cell carcinomas (LSCCs) and its significance
CONCLUSION: The above may suggest a role for claudin-1 in the development and progression of laryngeal squamous cell carcinoma. Overall, claudin-1's aberrant expression in laryngeal cancer is in line with evidence seen in other head and neck cancers. Its co-expression with slug/snail-1 in LSCC patients should be investigated further to understand the nature of the relationship of the two proteins in LSCC and their possible contribution to its development and progression.PMID:33629735 | DOI:10.14670/HH-18-320 (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 25, 2021 Category: Cytology Authors: Abderrahman Ouban Source Type: research

Traumatic brain injury: Estimate of the age of the injury based on neuroinflammation, endothelial activation markers and adhesion molecules
Histol Histopathol. 2021 Feb 24:18319. doi: 10.14670/HH-18-319. Online ahead of print.ABSTRACTStudies on traumatic brain injury (TBI) are applicable not only in the clinical context, but also in the forensic field. Over time, the literature has accumulated scientific evidence supporting the use of specific histopathological tests in dating traumatic brain injuries. In primary damage, cell death occurs by necrosis/apoptosis. In secondary injury, the underlying mechanisms are inflammation and ischemia. The inflammatory response of the central nervous system (CNS) follows the common steps of the innate response. In head injur...
Source: Histology and Histopathology - February 24, 2021 Category: Cytology Authors: Massimiliano dell'Aquila Aniello Maiese Alessandra De Matteis Rocco Valerio Viola Mauro Arcangeli Raffaele La Russa Vittorio Fineschi Source Type: research

Optimisation and validation of immunohistochemistry protocols for cancer research.
CONCLUSIONS: This study has optimised and standardized several tissue-based biomarkers that may be, in the future, used to screen, diagnose and monitor patients with certain cancer, such as bladder cancer. Accurate data on optimised protocols reduce time and resources wasted on experimental protocols, and ultimately help identify biomarkers or biomarker panels, which may be used to select treatment regimens for various cancers. PMID: 33616195 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 23, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Visual deficits after traumatic brain injury.
Discussion of current advancements in TBI animal models and the recent pathophysiological findings related to the neuro-glia-vascular unit (NVU) will help elucidate novel targets for potential therapeutics lines. Only over the past decade have newer pharmaceutical and stem cell-based treatments begun to come to light. The potency for these new lines of TBI specific curatives will be discussed along with the review of current blast-induced TBI models, providing potential directions for future research. PMID: 33599281 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 20, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Biological and biocompatible characteristics of fullerenols nanomaterials for tissue engineering.
Authors: Zhao Y, Shen X, Ma R, Hou Y, Qian Y, Fan C Abstract Fullerenes, as hydrophobic molecules, are limited in biomedical function due to their very low solubility. But taking C60 (OH) x as an example, the properties of fullerenols were analyzed. It was found that fullerenols had good stability, water solubility, good biocompatibility and low cytotoxicity by adding a hydroxyl group to carbon atoms. In the biomedical field, it has been found that fullerene C60 can be used as a powerful free radical scavenger, with antioxidant activity, with antibacterial and inhibitory effects on cancer cells. Fullerenols inherit...
Source: Histology and Histopathology - February 20, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Pericyte morphology and function.
Authors: Alarcon-Martinez L, Yemisci M, Dalkara T Abstract The proper delivery of blood is essential for healthy neuronal function. The anatomical substrate for this precise mechanism is the neurovascular unit, which is formed by neurons, glial cells, endothelia, smooth muscle cells, and pericytes. Based on their particular location on the vessel wall, morphology, and protein expression, pericytes have been proposed as cells capable of regulating capillary blood flow. Pericytes are located around the microvessels, wrapping them with their processes. Their morphology and protein expression substantially vary along t...
Source: Histology and Histopathology - February 19, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Low expression of FXYD5 reverses the cisplatin resistance of epithelial ovarian cancer cells.
CONCLUSION: FXYD5 downregulation may reduce the invasion, migration and EMT formation of EOC cells to increase their sensitivity to cisplatin chemotherapy by inhibiting cell proliferation and promoting cell apoptosis. PMID: 33570156 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 13, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Exposure to second-hand cigarette smoke exacerbates the progression of osteoarthritis in a surgical induced murine model.
Authors: Rose BJ, Weyand JA, Liu B, Smith JF, Perez BR, Clark JC, Goodman M, Hirschi Budge KM, Eggett DL, Arroyo JA, Reynolds PR, Kooyman DL Abstract Osteoarthritis (OA), formerly understood to be a result of passive wear, is now known to be associated with chronic inflammation. Cigarette smoking promotes systemic inflammation and has been implicated in increased joint OA incidence in some studies, though the recent observational data on the association are contradictory. We hypothesize that second-hand smoke (SHS) treatment will increase the incidence of OA in a mouse model that has been subjected to a surgical de...
Source: Histology and Histopathology - February 13, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Is there still a place for conventional histopathology in the age of molecular medicine? Laur én classification, inflammatory infiltration and other current topics in gastric cancer diagnosis and prognosis.
In this study, we discuss recent topics on the histological diagnosis of GC, focusing on the current role of Laurén classification and the potential value of new histological features in GC, such as inflammatory infiltration and tumor budding. PMID: 33565601 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 12, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Transient receptor potential (TRP) channels in human colorectal cancer: evidence and perspectives.
Authors: Rizopoulos T, Assimakopoulou M Abstract Colorectal cancer (CRC) is one of the leading causes of death in the civilized world. Transient receptor potential channels (TRPs) are a heterogeneous family of cation channels that play an important role in gastrointestinal physiology. TRPs have been linked with carcinogenesis in the colon and their role as potential therapeutic targets and prognostic biomarkers is under investigation. PMID: 33528023 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - February 3, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Patient iPSC-derived retinal organoids: Observable retinal diseases in-a-dish.
Authors: Zhang XH, Jin ZB Abstract Induced pluripotent stem cells (iPSCs), reprogrammed from human somatic cells, hold the capacity to differentiate into most human body cells. iPSCs can be differentiated into retinal organoids, a three-dimensional structured retina containing various retinal cells. Patient-specific retinal organoids provide a powerful disease model to recapitulate the disease to study the pathogenesis of inherited retinal dystrophies, to screen or discover new drugs, and most importantly to supply an unlimited cell source for retinal regeneration. PMID: 33491763 [PubMed - as supplied by publis...
Source: Histology and Histopathology - January 27, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

MiR-590-5p regulates cell proliferation, apoptosis, migration and invasion in oral squamous cell carcinoma by targeting RECK.
CONCLUSION: MiR-590-5p expression was obviously increased in OSCC, and inhibiting miR-590-5p enhanced the expression of its target gene RECK, thereby suppressing proliferation, migration and invasion of OSCC cells and promoting apoptosis of OSCC cells. PMID: 33447989 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 17, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

PDCD4 regulates apoptosis in human peritoneal mesothelial cells and promotes gastric cancer peritoneal metastasis.
CONCLUSIONS: These data suggested that decreased expression of PDCD4 significantly promoted apoptosis in human peritoneal mesothelial cells, thus inducing peritoneal metastasis, and that TGF-β1 secreted from gastric cancer cells may have played a crucial role. PMID: 33442866 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 16, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Aldehyde dehydrogenase 1B1 is a potential marker of colorectal tumors.
Authors: Wang H, Li Y, Zhou D, Li X, Jia S, Qi S, Huang J Abstract Colorectal cancer (CRC) is common worldwide. Aldehyde dehydrogenase 1B1 (ALDH1B1), a member of the ALDH1 family, serves as a biomarker for cancer stem cells. We hypothesized that ALDH1B1 expression is associated with colorectal tumors. Immunohistochemistry was used to detect ALDH1B1 expression across a commercial colorectal tissue microarray. The signal intensities of the positively stained tissues were expressed using the mean integrated optical density (mean IOD). We also analyzed ALDH1B1 mRNA expression in the Oncomine database. The associations ...
Source: Histology and Histopathology - January 14, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Morphological and histochemical changes in the dromedary camel epididymis in relation to reproductive activity.
Authors: Ibrahim ZH, Al-Kheraije KA, Singh SK Abstract Environmental conditions such as temperature, light and food availability are known to influence the physiological status of animals. The male dromedary camel (Camelus dromedarius) is considered as a seasonal breeder with maximal sexual activity during certain period of the year followed by a decrease in activity during the remaining period. On the other hand, the male camel is also shown as an atypical seasonal breeder because this does not undergo sexual quiescence with complete cessation of spermatogenesis. This animal, however, shows remarkable physiologica...
Source: Histology and Histopathology - January 13, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

An overview of the structural and functional aspects of immune cells in teleosts.
Authors: Mokhtar DM, Abdelhafez EA Abstract The immune system of fish consists of two main components, innate and adaptive immunities. Innate immunity is non-specific and acts as the primary line of protection against pathogen invasion, while adaptive immunity is more specific to a certain pathogen/following adaptation. The adaptive immune system consists of the humoral and cellular components. Cytotoxic T-lymphocyte cells are the major component of the cellular immunity that frequently kills viral-, bacterial- or parasitic-infected cells. According to the anatomical location, the mucosal-associated lymphoid tissue...
Source: Histology and Histopathology - January 10, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Propofol protects PC12 cells from cobalt chloride -induced injury by mediating miR-134.
CONCLUSION: PRO may mitigate CoCl2-induced autophagy in PC12 cells with decreased apoptosis and improved oxidative stress via mediating miR-134. PMID: 33410125 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 9, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Extracellular vesicles derived from mesenchymal stem cells: A platform that can be engineered.
Authors: Qin B, Zhang Q, Chen D, Yu HY, Luo AX, Suo LP, Cai Y, Cai DY, Luo J, Huang JF, Xiong K Abstract Mesenchymal stem cells play an important role in tissue damage and repair. This role is mainly due to a paracrine mechanism, and extracellular vesicles (EVs) are an important part of the paracrine function. EVs play a vital role in many aspects of cell homeostasis, physiology, and pathology, and EVs can be used as clinical biomarkers, vaccines, or drug delivery vehicles. A large number of studies have shown that EVs derived from mesenchymal stem cells (MSC-EVs) play an important role in the treatment of various ...
Source: Histology and Histopathology - January 7, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Farnesoid X receptor: a potential therapeutic target in multiple organs.
Authors: Zhang C, Wang Z, Feng Q, Chen WD, Wang YD Abstract Farnesoid X receptor (FXR), a member of the nuclear receptor family, is a common receptor found in the intestine and liver, and helps to maintain systemic metabolic homeostasis through regulating bile acid, glucose, lipid metabolism, and energy homeostatsis. In addition, FXR regulates the functions of various organs, such as liver, intestine, kidney, breast, pancreas, cardiovascular system and brain. FXR also plays a key role in regulation of gut-microbiota through mediating the various signaling pathways. Accordingly, FXR has become an attractive therapeu...
Source: Histology and Histopathology - January 6, 2021 Category: Cytology Tags: Histol Histopathol Source Type: research

Implications of androgen receptor and FUS expression on tumor progression in urothelial carcinoma.
CONCLUSION: FUS expression is associated with favorable prognostic parameters of UC. A possible interaction is suggested between FUS and AR pathways involved in urothelial cancer progression. Manipulating FUS levels and androgen deprivation therapy can provide new promising targets for treatment trials. PMID: 33354760 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 30, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Frequent DYRK2 gene amplification in micropapillary element of lung adenocarcinoma - an implication in progression in EGFR-mutated lung adenocarcinoma.
Authors: Koike C, Okudela K, Matsumura M, Mitsui H, Suzuki T, Arai H, Kataoka T, Ishikawa Y, Umeda S, Tateishi Y, Ohashi K Abstract The present study aimed to discern the molecular alterations involved in the progression of EGFR-mutated lung adenocarcinoma (LADC). We previously demonstrated that the micropapillary (mPAP) element is the most important histological factor for assessing malignant grades in LADCs. Therefore, mPAP and other elements were separately collected from three cases of EGFR-mutated LADC using laser capture microdissection and subjected to a comprehensive mRNA expression analysis. We focused on ...
Source: Histology and Histopathology - December 30, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Shufeng Jiedu capsules protect rats against LPS-induced acute lung injury via activating NRF2-associated antioxidant pathway.
In conclusion, SFJDC treatment may protect the rat lungs from LPS by alleviating the inflammation response via NRF2-associated antioxidant pathway. PMID: 33346364 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 22, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Myriad pillars formed by intussusceptive angiogenesis as the basis of intravascular papillary endothelial hyperplasia (IPEH). IPEH is intussusceptive angiogenesis made a lesion.
In conclusion, the formation of myriad pillars, predominantly in veins, is the basis of IPEH. This lesion may therefore be considered an excessive expression of IA: IA becomes a lesion. PMID: 33336356 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 20, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Argentic staining reveals changes in cerebellar tissue organisation by prenatal glucocorticoid administration in rats.
Authors: Rivas-Manzano P, Ramírez-Escoto MM, De la Rosa-Rugerio C, Rugerio-Vargas C, Ortiz-Hernández R, Torres-Ramírez N Abstract It was almost 150 years ago that Golgi revolutionised histology with silver-based stains. Major advances in knowledge of the nervous system became possible because of silver impregnations. Silver staining combined with classical histological staining, cytochemistry methods, and electron microscopy is useful for studying mechanisms and components at subcellular, cellular, and tissue levels. Despite the advantages of silver staining, its use has decreased over time. Th...
Source: Histology and Histopathology - December 18, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Effect of sulfur dioxide on vascular biology.
Authors: Cai H, Wang X Abstract Gasotransmitters, such as nitric oxide, carbon monoxide and hydrogen sulfide, can be generated endogenously. These gasotransmitters play important roles in vascular biology, including vasorelaxation and inhibition of vascular smooth muscle cell (VSMC) proliferation. In recent years, sulfur dioxide (SO₂) has been considered as a fourth gasotransmitter. SO₂ is present in air pollution. Moreover, SO₂ toxicity, including oxidative stress and DNA damage, has been extensively reported in previous studies. Recent studies have shown that SO₂ can be endogenously generated in various o...
Source: Histology and Histopathology - December 17, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

The pathogenesis and pathology of idiopathic pleuroparenchymal fibroelastosis.
Authors: Kinoshita Y, Ishii H, Nabeshima K, Watanabe K Abstract Idiopathic pleuroparenchymal fibroelastosis (IPPFE) is a rare subtype of idiopathic interstitial pneumonias that consists of elastofibrosis involving the lung parenchyma and pleural collagenous fibrosis predominantly located in the upper lobes. IPPFE has various distinct clinical and physiological characteristics, including platythorax and a marked decrease of forced vital capacity with an increased residual volume on a respiratory function test. The concept of IPPFE is now widely recognized and some diagnostic criteria have been proposed. In addition,...
Source: Histology and Histopathology - December 15, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

CORRIGENDUM TO: "Preterm birth and/or low birth weight are associated with periodontal disease and the increased placental immunohistochemical expression of inflammatory markers".
CORRIGENDUM TO: "Preterm birth and/or low birth weight are associated with periodontal disease and the increased placental immunohistochemical expression of inflammatory markers". Histol Histopathol. 2020 Oct;35(10):1211 Authors: Pozo E, Mesa F, Ikram MH, Puertas A, Torrecillas-Martínez L, Ortega-Oller I, Magán-Fernández A, Rodríguez-Martínez MD, Padial-Molina M, Sánchez-Fernández E, Galindo-Moreno P, O'Valle F Abstract This correct the article DOI: 10.14670/HH-11-671. PMID: 33305349 [PubMed - in process] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 13, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Pancreatic neuroendocrine neoplasms: Clinicopathological features and pathological staging.
Authors: Lam AK, Ishida H Abstract The nomenclature and classification of pancreatic neuroendocrine neoplasms has evolved in the last 15 years based on the advances in knowledge of the genomics, clinical behaviour and response to therapies. The current 2019 World Health Organization classification of pancreatic neuroendocrine neoplasms categorises them into three groups; pancreatic neuroendocrine tumours (PanNETs)(grade 1 grade 2, grade 3), pancreatic neuroendocrine carcinomas and mixed neuroendocrine-non-neuroendocrine neoplasms (MiNENs) based on the mitotic rate, Ki-67 index, morphological differentiation and/or ...
Source: Histology and Histopathology - December 13, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Paracervical ganglion in the female pig during prenatal development: Morphology and immunohistochemical characteristics.
In this study, for the first time, the morphology of PCG formation in the porcine foetus has been described in three stages of development. Dynamic changes in the number of neurons and their sizes were also noted, as well as the changes in immunochistochemical coding of maturing neurons. PMID: 33269806 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 5, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Targeting NRF2 to suppress ferroptosis in brain injury.
Authors: Song S, Gao Y, Sheng Y, Rui T, Luo C Abstract Brain injury is accompanied by serious iron metabolism disorder and oxidative stress. As a novel form of regulated cell death (RCD) depending on lipid peroxidation caused by iron overload, ferroptosis (FPT) further aggravates brain injury, which is different from apoptosis, autophagy and other traditional cell death in terms of biochemistry, morphology and genetics. Noteworthy, transcriptional regulator NRF2 plays a key role in the cell antioxidant system, and many genes related to FPT are under the control of NRF2, including genes for iron regulation, thiol-de...
Source: Histology and Histopathology - November 28, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Astrocyte phenotypes: Emphasis on potential markers in neuroinflammation.
Authors: Bozic I, Savic D, Lavrnja I Abstract Astrocytes, the most abundant glial cells in the central nervous system (CNS), have numerous integral roles in all CNS functions. They are essential for synaptic transmission and support neurons by providing metabolic substrates, secreting growth factors and regulating extracellular concentrations of ions and neurotransmitters. Astrocytes respond to CNS insults through reactive astrogliosis, in which they go through many functional and molecular changes. In neuroinflammatory conditions reactive astrocytes exert both beneficial and detrimental functions, depending on the...
Source: Histology and Histopathology - November 25, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Outside-in signaling by femoral cuff injury induces a distinct vascular lesion in adipose triglyceride lipase knockout mice.
We report the effect of cuff injury on femoral arteries of ATGL-knockout (ATGL⁻/⁻) mice. Cuff-induced concentric neointimal formation with migrating SMCs was exacerbated in ATGL⁻/⁻ mice, mimicking atherosclerotic lesions in patients with TGCV. In the media, cell death of SMCs and loss of elastic fibers increased. Perivascular infiltrating cells expressing tumor necrosis factor-α (TNF-α) were more prominent in ATGL⁻/⁻ mice than in wild-type (WT) mice. In Boyden chamber experiments, a greater number of ATGL⁻/⁻ SMCs migrated in response to TNF-α compared to WT SMCs. These data, for the first ...
Source: Histology and Histopathology - November 25, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

The BRD4 inhibitor JQ1 protects against chronic obstructive pulmonary disease in mice by suppressing NF- κB activation.
CONCLUSION: The BRD4 inhibitor JQ1 can downregulate MMP-2 and MMP-9 expression, reduce inflammatory responses, and alleviate oxidative stress in mice with COPD, and this mechanism might be related to the inhibition of NF-κB. PMID: 33215396 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - November 21, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Expressions of IL-8 and CXCL5 in uterine endometrioid carcinomas which have frequent neutrophil infiltration and comparison to colorectal adenocarcinoma.
In this study, we investigated IL-8, CXCL5 and C-X-C motif chemokine receptor 2 (CXCR2) (their chemokine receptor) expressions in ECs by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). There are few reports on the relationship between these chemokines and ECs. For comparison, we enrolled samples of colorectal adenocarcinoma (CRAC), it is another representative tumor with neutrophil infiltration. We analyzed 30 ECs and 30 CRACs. We confirmed IL-8 expression (H-score ≥50 points) in 40% of EC and 7% of CRAC samples; CXCL5 expression in 7% of EC and 10% of CRAC samples; CXCR2 expression in...
Source: Histology and Histopathology - November 17, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Sympathetic nervous system contributes to orthodontic tooth movement by central neural regulation from hypothalamus.
In conclusion, this study provided the first evidence for the involvement of the hypothalamus in the promotion effect of SNS on OTM. This work could provide a novel theoretical and experimental basis for further understanding of the molecular mechanism of OTM. PMID: 33179759 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - November 14, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research