Mechanisms of neuroinflammation and inflammatory mediators involved in brain injury following subarachnoid hemorrhage.
Authors: Okada T, Suzuki H Abstract Subarachnoid hemorrhage (SAH) is a devastating cerebrovascular disorder. Neuroinflammation is a critical cause of brain injury following SAH in both acute and chronic phases. While accumulating evidence has shown that therapies targeting neuroinflammation exerted beneficial effects in experimental SAH, there is little clinical evidence. One of the factors making neuroinflammation complicated is that inflammatory signaling pathways and mediators act as protective or detrimental responses at different phases. In addition, biomarkers to detect neuroinflammation are little known in c...
Source: Histology and Histopathology - February 8, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

CSN6: a promising target for cancer prevention and therapy.
Authors: Hou J, Cui H Abstract CSN6 has recently received increased attention as a multifunctional protein involved in protein stability. CSN6 plays an important role in controlling cellular proliferation, apoptosis and metastasis, modulating signal transduction, as well as regulating DNA damage and repair. Most studies have demonstrated that CSN6 is significantly upregulated in human malignant tumors such as cervical cancer, papillary thyroid cancer, colorectal cancer, breast cancer, lung adenocarcinoma, and glioblastoma, and its expression is usually correlated with poor prognosis. In this review, we summarize re...
Source: Histology and Histopathology - February 7, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Growth pattern of experimental glioblastoma.
Authors: Ahlstedt J, Förnvik K, Helms G, Salford LG, Ceberg C, Skagerberg G, Redebrandt HN Abstract Glioblastoma multiforme (GBM) is an aggressive primary brain malignancy with a very poor prognosis. Researchers employ animal models to develop potential therapies. It is important that these models have clinical relevance. This means that old models, propagated for decades in cultures, should be questioned. Parameters to be evaluated include whether animals are immune competent or not, the infiltrative growth pattern of the tumor, tumor volume resulting in symptoms and growth rate. We here describe the growth p...
Source: Histology and Histopathology - February 7, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Histological villous maturation in placentas of complicated pregnancies.
Authors: Vangrieken P, Vanterpool SF, van Schooten FJ, Al-Nasiry S, Andriessen P, Degreef E, Alfer J, Kramer BW, von Rango U Abstract Chorioamnionitis and preeclampsia account for the majority of preterm births worldwide. Thus far, adequate methods for early detection or prevention of these diseases are lacking. In preeclampsia, accelerated villous maturation is believed to compensate placental insufficiency. However, little is known about the effects of placental inflammation in chorioamnionitis on villous maturation. Therefore, we established a set of morphological parameters to evaluate histological villous matu...
Source: Histology and Histopathology - January 29, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Cathepsin-B dependent autophagy ameliorates steatoheaptitis in chronic exercise rats.
CONCLUSIONS: Chronic aerobic exercise reduces oxidative stress/ROS and ROS may cause lysosomal membrane destabilisation and disrupts the autophagic process. The beneficial effect of chronic exercise may further inhibit the process of lysosome membrane permeabilisation and facilitate lysosome fusion with autophagosomes to trigger autophagy. This process may possibly contribute to the inhibition of cathepsin B released into cytosol which further reduces inflammation and mitochondrial-dependent apoptosis. PMID: 31975365 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 26, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Overexpression of YES1 is associated with favorable prognosis and increased platinum-sensitivity in patients with epithelial ovarian cancer.
CONCLUSIONS: High YES1 cytoplasmic expression in EOC patient tissue is significantly correlated with favorable prognosis. Patients with high YES1 expression tend to be sensitive to platinum-based chemotherapy. PMID: 31970720 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 25, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Chaperone-mediated autophagy in cancer: Advances from bench to bedside.
Authors: Hou T, Fan Y, Dan W, Liu B, Wang Z, Zeng J, Li L Abstract Chaperone-mediated autophagy (CMA), a selective form of autophagy, where cellular proteins with KFERQ-like motif are targeted to the lysosome for degradation, is necessary to maintain cellular homeostasis. The role of CMA in neurodegenerative diseases has been extensively studied in the past decades, with defects in the pathway being strongly associated with disease. Recently, accumulating evidence has demonstrated a consistent increase in basal CMA activity in a wide array of cancer cell lines and human tumor biopsies, suggesting a potential link b...
Source: Histology and Histopathology - January 23, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Spatial and phenotypic characterization of pancreatic cancer-associated fibroblasts after neoadjuvant treatment.
Authors: Nielsen MFB, Mortensen MB, Sørensen MD, Wirenfeldt M, Kristensen BW, Schrøder HD, Pfeiffer P, Detlefsen S Abstract Pancreatic ductal adenocarcinoma (PC) is characterized by a highly fibrotic desmoplastic stroma. Subtypes of cancer-associated fibroblasts (CAFs) have been identified in chemotherapy-naïve PC (CTN-PC), but their precise functions are still unclear. Our knowledge regarding the properties of CAFs in the regressive stroma after neoadjuvant treatment (NAT) of PC (NAT-PC) is particularly limited. We aimed to examine the marker phenotypic properties of CAFs in the regressive strom...
Source: Histology and Histopathology - January 22, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Pancreas is a preeminent source of ghrelin after sleeve gastrectomy in Wistar rats.
We reported a significant increase of ghrelin epsilon-cells in the pancreas and basal serum after Sleeve gastrectomy versus the control groups. The epsilon cellular increment was related to neogenesis, as the neurogenin-3 marker revealed. The Roux-en Y Gastric Bypass showed neither epsilon cell increase nor basal serum changes in ghrelin release. As a conclusion, we reported that the severe suppression of the fundus gastric produced the recovery of ghrelin released by the epsilon cells, which was indicative of an ontogenic embryonic pancreatic function. PMID: 31951010 [PubMed - as supplied by publisher] (Source: Histol...
Source: Histology and Histopathology - January 19, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

How galectins have become multifunctional proteins.
Authors: García Caballero G, Kaltner H, Kutzner TJ, Ludwig AK, Manning JC, Schmidt S, Sinowatz F, Gabius HJ Abstract Having identified glycans of cellular glycoconjugates as versatile molecular messages, their recognition by sugar receptors (lectins) is a fundamental mechanism within the flow of biological information. This type of molecular interplay is increasingly revealed to be involved in a wide range of (patho)physiological processes. To do so, it is a vital prerequisite that a lectin (and its expression) can develop more than a single skill, that is the general ability to bind glycans. By studying the...
Source: Histology and Histopathology - January 12, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Effects of estrogen on STIM1/Orai1 in the sublingual gland of ovariectomized rats.
CONCLUSION: This study demonstrates that low estrogen status significantly reduced the expression of STIM1 and Orai1 in the sublingual gland of rats, along with cellular Ca²⁺ levels. These data provide insight into the likely mechanisms underlying sublingual gland secretion dysfunction during menopause. PMID: 31916583 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - January 11, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Downregulation of nuclear ING3 expression and translocalization to cytoplasm promotes tumorigenesis and progression in head and neck squamous cell carcinoma (HNSCC).
In conclusion, decreases in nuclear ING3 may play important roles in tumorigenesis, progression and tumor differentiation in HNSCC. Increases in cytoplasmic ING3 may be due to 14-3-3η binding and may also be involved in malignant progression. Nuclear ING3 may modulate the transactivation of target genes, promoting apoptosis through interactions with p300 and p21. Moreover, ING3 may interact with p300 to upregulate the level of acetylation of p53, and promote p53-mediated cell cycle arrest, senescence and/or apoptosis. Therefore, ING3 may be a potential tumor suppressor and a possible therapeutic target in HNSCC. PM...
Source: Histology and Histopathology - January 1, 2020 Category: Cytology Tags: Histol Histopathol Source Type: research

Therapeutic effects of platelet derived growth factor overexpressed-mesenchymal stromal cells and sheets in canine skin wound healing model.
Authors: Kim N, Choi KU, Lee E, Lee S, Oh J, Kim WK, Woo SH, Kim DY, Kim WH, Kweon OK Abstract Adipose-derived mesenchymal stromal cells (Ad-MSCs) have excellent potential for skin wound repair. Moreover, platelet-derived growth factor (PDGF) has strong wound healing properties. The purpose of the present study was to compare the healing effects of PDGF-overexpressing canine allogeneic Ad-MSCs (PDGF-MSCs) and their cell sheets (PDGF-CSs) as compared to unexpressed Ad-MSCs (U-MSCs) and their cell sheets (UCSs) in a cutaneous wound healing model induced upon dogs. In in vitro study, the expression of immunomodulatory...
Source: Histology and Histopathology - December 27, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Next generation sequencing identifies novel potential actionable mutations for grade I meningioma treatment.
Authors: Pepe F, Pisapia P, de Caro MLDB, Conticelli F, Malapelle U, Troncone G, Martinez JC Abstract Meningiomas are common brain tumors that arise from the meningeal membranes that envelope the brain and spinal cord. The World Health Organization classifies these tumors into three histopathological grades. Because of tumor recurrence, treating meningiomas may be challenging even in well-differentiated grade I (GI) neoplasms. Indeed, around 5% of completely resected GI meningiomas relapse within 5 years. Therefore, identifying driver mutations in GI meningiomas through next generation sequencing (NGS) assays is pa...
Source: Histology and Histopathology - December 26, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Hepatocellular carcinoma in non-alcoholic fatty liver disease (NAFLD) - pathological evidence for a predominance of steatohepatitic/ inflammatory non-proliferative subtype.
CONCLUSION: NAFLD-related HCC was found in non-cirrhotic patients in 42% of cases, alpha-fetoprotein level was normal in 63% and "steatohepatitic HCC" was the predominant histological type. Immunoexpression of K19 and/or Ki-67 occurred in 32% of the nodules and were associated with intratumoral inflammation and ballooning, suggesting that HCC in MtS may be preferentially "an inflammatory, non-proliferative subtype of HCC". PMID: 31858523 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 22, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Effect of short-term betamethasone administration on the regeneration process of tissue-engineered bone.
Authors: Chihara T, Zhang Y, Li X, Shinohara A, Kagami H Abstract Local inflammation at the transplanted site of tissue-engineered bone may cause apoptosis of the transplanted cells, thus negatively affecting bone regeneration. To maximize the efficacy of bone tissue engineering, the local effect of short-term corticosteroid administration at the transplanted site of tissue-engineered bone was studied with respect to the expression of inflammatory cytokines. Compact bone-derived cells from mouse leg bones were isolated, cultured and seeded onto β-tricalcium phosphate granules. The constructs were transplanted ...
Source: Histology and Histopathology - December 20, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Therapeutic effects of an azaphenothiazine derivative in mouse experimental colitis.
In conclusion we demonstrated a therapeutic efficacy of the compound in amelioration of local and systemic pathological changes associated with chemically-induced colitis in mice. A possible mechanism of action of the compound is discussed. PMID: 31833559 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - December 15, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

The role of mitochondrial fusion and fission in the process of cardiac oxidative stress.
Authors: Yu F, Abdelwahid E, Xu T, Hu L, Wang M, Li Y, Mogharbel BF, de Carvalho KAT, Guarita-Souza LC, An Y, Li P Abstract Mitochondria are the energy suppliers in the cell and undergo constant fusion and fission to meet metabolic demand during the cell life cycle. Well-balanced mitochondrial dynamics are extremely important and necessary for cell survival as well as for tissue homeostasis. Cardiomyocytes contain large numbers of mitochondria to satisfy the high energy demand. It has been established that deregulated processes of mitochondrial dynamics play a major role in myocardial cell death. Currently, cardiac...
Source: Histology and Histopathology - December 12, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Triptolide inhibits tonsillar IgA production by upregulating FDC-SP in IgA nephropathy.
Authors: Li H, Yang X, Yao G, Zhang Y, Xu Y, Cao Y, An X, Li H, Chen H, Geng J, Yuan D, Jin X, Meng H Abstract IgA nephropathy (IgAN) is primarily resulted of qualitative abnormality of IgA. The occurrence of IgAN is associated with affected tonsils which enhances the IgA production via IgA class switching and immuno-activation. Follicular dendritic cell-secreted protein (FDC-SP) was found to be a negative effect for IgA production in tonsil. The previous studies suggested that Triptolide might reduce IgA production by its immunosuppression role. Given this background, this study investigated the mechanisms underly...
Source: Histology and Histopathology - December 10, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Role of androgen receptor expression in non-muscle-invasive bladder cancer: a systematic review and meta-analysis.
Authors: Sanguedolce F, Cormio L, Carrieri G, Calò B, Russo D, Menin A, Pastore AL, Greco F, Bozzini G, Galfano A, Pini G, Porreca A, Mugavero F, Falsaperla M, Ceruti C, Cindolo L, Antonelli A, Minervini A Abstract In order to evaluate the potential prognostic/predictive role of androgen receptor (AR) expression in non-muscle-invasive bladder cancer (NMIBC), and whether it may represent a therapeutic target, we conducted a systematic search of the literature using 'androgen receptor or AR', 'testosterone', 'bladder cancer' and 'non-muscle invasive bladder cancer or NMIBC' as keywords. Eleven studies met the ...
Source: Histology and Histopathology - December 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

The regulatory roles and potential prognosis implications of long non-coding RNAs in gastric cancer.
Authors: Wang Y, Yang F, Yang Q Abstract Accumulating dysregulated lncRNAs have been demonstrated to execute vital functions in the pathogenesis and progress of gastric cancer (GC) through versatile molecular mechanisms. In this review, we classify the mechanisms of dysregulated lncRNAs in GC into several governing types according to their roles at molecular level. For each regulatory role, we illustrate several instructive examples and introduce significant effects of lncRNAs on cellular biological properties of GC. Besides, we summarize a group of lncRNA-signatures that are potential biomarkers in the prediction ...
Source: Histology and Histopathology - December 5, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Chronic atrophic gastritis aggravate chronic periodontitis with Helicobacter pylori infection and CD4+Th cytokines infiltration.
CONCLUSIONS: Datients with CAG demonstrated that significant elevated attachment loss in periodontitis lesions, while elevated Th17 cytokines IL-17, IL-21 and IL-23 participate in immunopathogenesis of both diseases. PMID: 31749138 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - November 23, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Expression of glucose-regulated protein 78 as prognostic biomarkers for triple-negative breast cancer.
Authors: Yang C, Zhang Z, Zou Y, Gao G, Liu L, Xu H, Liu F Abstract INTRODUCTION: glucose-regulated protein78(GRP78) is a stress - induced endoplasmic reticulum chaperone protein. it is closely related to the occurrence, development, proliferation, differentiation and drug resistance of breast cancer. However, the association and clinicopathological features between GRP78 and triple negative breast cancer (TNBC) remain to be studied. MATERIAL AND METHODS: Clinical and pathological characteristics and overall survival were analysed retrospectively in 179 surgically resected TNBC patients. GRP78 was detected by i...
Source: Histology and Histopathology - November 22, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Overexpression of EIF5A2 is associated with poor survival and aggressive tumor biology in gallbladder cancer.
In this study, our results showed for the first time that EIF5A2 was overexpressed in GBC samples compared with non-tumor tissue. Overexpression of EIF5A2 was associated with lymph node metastasis, tumor differentiation, UICC (Union for International Cancer Control) staging, histological type, metastasis, and tumor size. Overexpression of EIF5A2 in gallbladder carcinoma tissues is also associated with poor prognosis in patients. The interference of EIF5A2 significantly inhibited the proliferation, cell cycle, migration and colony formation of GBC-SD cells in vitro. Our results suggest that EIF5A2 is a target oncogene and m...
Source: Histology and Histopathology - November 22, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Use of nanoparticles in skeletal tissue regeneration and engineering.
Authors: Filippi M, Born G, Felder-Flesch D, Scherberich A Abstract Bone and osteochondral defects represent one of the major causes of disabilities in the world. Derived from traumas and degenerative pathologies, these lesions cause severe pain, joint deformity, and loss of joint motion. The standard treatments in clinical practice present several limitations. By producing functional substitutes for damaged tissues, tissue engineering has emerged as an alternative in the treatment of defects in the skeletal system. Despite promising preliminary clinical outcomes, several limitations remain. Nanotechnologies could ...
Source: Histology and Histopathology - November 15, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

PTK7 expression is associated with lymph node metastasis, ALK and EGFR mutations in lung adenocarcinomas.
Authors: Jiang W, He J, Lv B, Xi X, He G, He J Abstract Non-small cell lung cancer (NSCLC) is one of the leading causes of cancer death worldwide. Lung adenocarcinoma is the main tumor type of NSCLC. Recent advances in the molecular characterization and personalized therapies have improved NSCLC patient prognosis. Previous studies showed that protein tyrosine kinase 7 (PTK7) plays an important role in human cancers. However, the role of PTK7 has not been investigated. PTK7 expression was assessed by immunohistochemistry in 95 patients with lung adenocarcinoma. Correlations of PTK7 expression levels with clinicopath...
Source: Histology and Histopathology - November 10, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Ewing's sarcoma of the cervix: A case report and review of literature.
We report a 76-year-old woman who had groin mass. ES was diagnosed in accordance with morphological and immunohistochemical maps. Fluorescence in situ hybridization and RT-PCR (reverse transcription PCR) revealed ESWR1 gene rearrangement and fusion gene formation (EWS-FLI-1), both of which confirmed the diagnosis of ES. Although the patient underwent surgical resection, the patient died without chemotherapy and radiotherapy. This case is the first one to involve a patient aged over 70 years and the fifth one to show metastasis occurrence. PMID: 31688946 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - November 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

The regulatory role of HIF-1 in tubular epithelial cells in response to kidney injury.
Authors: Qiu Y, Huang X, He W Abstract The high sensitivity to changes in oxygen tension makes kidney vulnerable to hypoxia. Both acute kidney injury and chronic kidney disease are almost always accompanied by hypoxia. Tubular epithelial cells (TECs), the dominant intrinsic cells in kidney tissue, are believed to be not only a victim in the pathological process of various kidney diseases, but also a major contributor to kidney damage. Hypoxia inducible factor-1 (HIF-1) is the main regulator of adaptive response of cells to hypoxia. Under various clinical and experimental kidney disease conditions, HIF-1 plays a piv...
Source: Histology and Histopathology - November 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Moderate-to-strong expression of FGFR3 and TP53 alterations in a subpopulation of choroid plexus tumors.
CONCLUSIONS: FGFR3 and FGFR1 proteins are expressed in a subpopulation of choroid plexus tumors. Further studies using larger cohorts of patients will allow identification of the clinicopathological implications of FGFR1 and FGFR3 expression in choroid plexus tumors. PMID: 31660579 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - October 31, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

CK20 and lymph node involvement predict adverse outcome of malignant intraductal papillary neoplasm of the bile duct.
CONCLUSIONS: Lymph node involvement and positive expression of CK20 are independent prognostic factors for shorter OS of m-IPNB, while patients with lymph node involvement and positive resection margin are at higher risk of tumor recurrence. PMID: 31657857 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - October 30, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

p66Shc regulates podocyte autophagy in high glucose environment through the Notch-PTEN-PI3K/Akt/mTOR pathway.
CONCLUSION: These findings indicate that p66Shc inhibits podocyte autophagy and induces apoptosis through the Notch -PTEN-PI3K/Akt/ mTOR signaling pathway in high glucose environment, providing novel evidence for its potential role in DKD treatment. PMID: 31650524 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - October 28, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Reduced histologic neo in-stent restenosis after use of a paclitaxel-coated cutting balloon in porcine coronary arteries.
Authors: Traxler D, Hemetsberger R, Spannbauer A, Zlabinger K, Gugerell A, Lukovic D, Mandic L, Pavo N, Winkler J, Gyöngyösi M Abstract The incidence of in-stent restenosis (ISR) has declined dramatically, but once it develops, no current treatment option, such as drug-eluting stents, drug-coated balloons, or cutting balloons (CBs), prevents re-narrowing of the stented atherosclerotic artery. In this preclinical study, we aimed to improve the efficacy of ISR treatment by coating CBs with paclitaxel (paclitaxel-eluting cutting balloon; PECB) and to characterize the histological features of neo-ISRs that ar...
Source: Histology and Histopathology - October 26, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Clinical significance of SPRY4-IT1 in efficacy and survival prediction in breast cancer patients undergoing neoadjuvant chemotherapy.
In this study, SPRY4-IT1 was significantly more expressed in cancer tissues than in normal tissues (P
Source: Histology and Histopathology - October 24, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Oral sarcomatoid squamous cell carcinoma: a retrospective study based on 14 cases.
Authors: Liu J, Xiao M, Wang Y Abstract The treatment outcomes for oral sarcomatoid squamous cell carcinoma (OSSCC) are far from satisfactory in our hospital. The aim of this study was to retrospectively summarize the OSSCC cases admitted to our department. From 2003 to 2017, 14 patients were hospitalized and diagnosed with OSSCC. We summarized and analysed the medical histories, diagnostic examinations, treatment strategies, and clinical outcomes of the involved cases. Of the 14 cases, 8 were located in the gingiva. The imageological diagnosis identified the existence of a mass with an infiltrative morphology pre-...
Source: Histology and Histopathology - October 24, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Silencing of Synaptotagmin 7 regulates osteosarcoma cell proliferation, apoptosis, and migration.
In this study, we aimed to investigate the role of SYT7 in osteosarcoma. METHODS: We defined the expression levels of SYT7 in osteosarcoma tissues and para-sarcoma tissues by immunohistochemistry and analyzed the possible correlation between SYT7 expression and pathological characteristics via Mann-Whitney U analysis and Spearman correlation analysis. The effects of SYT7 silencing in vitro cell growth were assessed by MTT assay. Cell cycle and cell apoptosis were assessed by flow cytometry analysis. Wound healing assay and transwell assay were applied to assess the migration and invasion capacity. RESULTS: The resu...
Source: Histology and Histopathology - October 23, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Aliskiren improves renal morphophysiology and inflammation in Wistar rats with 2K1C renovascular hypertension.
Authors: Pereira PG, Rabelo K, da Silva JFR, Ciambarella BT, Argento JGC, Nascimento ALR, Vieira AB, de Carvalho JJ Abstract Hypertension is characterized by persistent elevated blood pressure levels, one of the leading causes of death in the world. Renovascular hypertension represents the most common cause of secondary hypertension, and its progress is associated with overactivation of the renin angiotensin aldosterone system (RAAS), causing systemic and local changes. Aliskiren is a renin-inhibiting drug that optimizes RAAS suppression. In this sense, the objective of the present study was to analyze the morphoph...
Source: Histology and Histopathology - October 20, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Bovine oviduct epithelial cells suppress the phagocytic activity of neutrophils towards sperm but not for bacteria in vitro: Immunofluorescence and electron microscopic observations.
This study aimed to visually investigate the role of oviduct epithelium in regulating the phagocytic behavior of PMNs toward sperm as a physiological stimulus, with Escherichia coli (E. coli) as a pathological stimulus. In our experiment, PMNs were incubated for 2 h in BOEC-CM. Phagocytosis was then assayed by co-incubation of these PMNs either with sperm, E. coli, or latex beads. BOEC-CM significantly suppressed the direct phagocytosis of PMNs for sperm, but did not affect their phagocytic activity for E. coli or latex beads. Additionally, an investigation with scanning electron microscopy revealed that BOEC-CM suppressed...
Source: Histology and Histopathology - October 19, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Fiber type diversity in skeletal muscle explored by mass spectrometry-based single fiber proteomics.
Authors: Schiaffino S, Reggiani C, Murgia M Abstract Mammalian skeletal muscles are composed of a variety of muscle fibers with specialized functional properties. Slow fibers are suited for long lasting and low intensity contractile activity, while various subtypes of fast fibers are optimized to produce high force and power even with a significant fatigue. The functional specialization of muscle fibers is based on selective gene expression regulation, which provides each fiber with a specific protein complement. The recent refinement of small-scale sample preparation, combined with the development of mass spectrom...
Source: Histology and Histopathology - October 17, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Roles of microRNAs as non-invasive biomarker and therapeutic target in colorectal cancer.
Authors: Wan TM, Iyer DN, Ng L Abstract Evolutionary medicine has proven helpful to understand the origin of human disease, e.g. in identifying causal roles of recent environmental changes impacting on human physiology (environment-phenotype mismatch). In contrast, diseases affecting only a limited number of members of a species often originate from evolutionary trade-offs for usually physiologic adaptations assuring reproductive success in the context of extrinsic threats. For example, the G1 and G2 variants of the APOL1 gene supporting control of Trypanosoma infection come with the trade-off that they promote the...
Source: Histology and Histopathology - October 17, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Potential therapeutic effect of SO ₂ on fibrosis.
Potential therapeutic effect of SO₂ on fibrosis. Histol Histopathol. 2019 Oct 11;:18169 Authors: Wang XB, Cui H, Du JB Abstract Fibrosis is a pathological feature of most chronic diseases and leads to the dysfunction of various organs. However, there is currently no effective method for treating fibrosis. In recent years, a small gas, sulfur dioxide (SO₂), which can be generated endogenously in mammals, has been found to have vasorelaxation activity, improve cardiac function and decrease myocardial injury. Endogenous SO₂ also mediates the process of fibrosis. Inhibition of endogenous SO₂ can ag...
Source: Histology and Histopathology - October 13, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

The relationship between members of the canonical NF-kB pathway, tumour microenvironment and cancer specific survival in colorectal cancer patients.
CONCLUSION: Cytoplasmic pTAK1 was significantly associated with CSS and this was enhanced in patients with tumours that expressed wild type BRAF. High expression of cytoplasmic IKKβ was significantly associated with decreased CSS and with markers of the tumour microenvironment. These results support the hypothesis that NF-κB pathway members are poor prognostic markers in patients with CRC, but this requires to be validated in a large independent cohort. PMID: 31592535 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - October 10, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Pyloric and foveolar type metaplasia are important diagnostic features in Crohn's disease that are frequently missed in routine pathology.
Authors: Meditskou S, Grekou A, Toskas A, Papamitsou T, Miliaras D Abstract Pyloric type metaplasia (PYME) as evidence of chronic mucosal damage, is one of the main histopathological findings for diagnosing Crohn's Disease (CD) in terminal ileum biopsies, according to the latest guidelines but still frequently underdiagnosed in routine pathology. Foveolar metaplasia (FOME) changes in mucosa, another aspect of the chronic post -inflammatory Ulcer Associated Cell Lineage (UACL), have only been reported in a few cases. However, their clinical significance has not been investigated in depth except in pouchitis. The aim...
Source: Histology and Histopathology - September 21, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

The role of the non-ciliated bronchiolar cell in tolerance to inhaled vanadium of the bronchiolar epithelium.
Authors: López-Valdez N, Guerrero-Palomo G, Rojas-Lemus M, Bizarro-Nevares P, Gonzalez-Villalva A, Ustarroz-Cano M, Rivera-Fernández N, Fortoul TI Abstract The Non-Ciliated Bronchiolar Cell (NCBC) is responsible for the defense and maintenance of the bronchiolar epithelium. Several cellular defense mechanisms have been associated with an increase in the secretion of CC16 and changes in the phenotype of the cell; these mechanisms could be linked to tolerance to the damage due to exposure to inhaled Particulate Matter (PM) of the epithelium. These defense mechanisms have not been sufficiently explored. ...
Source: Histology and Histopathology - September 20, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Distribution and microstructure of intrarenal arteries in Bactrian Camels (Camelus Bactrianus).
Authors: Li H, Cui Y, Wang Y, Qiu H, Afedo SY, Huang Y, Bai X Abstract Studies and reports focusing on the Bactrian camels' kidney structure from an anatomical perspective are scanty, therefore, this work aims to systemically investigate the anatomical structure of the kidney and examine the distribution and microstructure of intrarenal arteries. Ten pairs of healthy adult kidneys from male and female Bactrian camels were used in the study. The kidney of Bactrian camel appeared like a broad bean with a smooth surface. Using artery casting, we observed that the renal artery divided into dorsal and ventral branches; ...
Source: Histology and Histopathology - September 20, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Histology of skeletal muscle reconstructed by means of the implantation of autologous adipose tissue: an experimental study.
Authors: Leiva-Cepas F, Jimena I, Ruz-Caracuel I, Luque E, Villalba R, Peña-Amaro J Abstract The purpose of this study was to determine the histological characteristics of a skeletal muscle reconstructed by means of the implantation of autologous adipose tissue following an experimentally-induced volumetric muscle loss. A cylindrical piece in the belly of the rat anterior tibial muscle was removed. In the hole, inguinal subcutaneous adipose tissue of the same rat was grafted. Animals were sacrificed 7, 14, 21, 28 and 60 days posttransplantation. Histological, histochemical, immunohistochemical and morphometr...
Source: Histology and Histopathology - September 17, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Diagnostic utility of CD205 in breast cancer: Simultaneous detection of myoepithelial cells and dendritic cells in breast tissue by CD205.
The objective of this study was to re-evaluate CD205 co-expression with other MEC markers, such as p63 and CD10, in nonneoplastic and neoplastic breast tissue and to evaluate its pathological diagnostic utility in these types of breast cancer. MATERIAL AND METHODS: Nonneoplastic breast tissue samples with a terminal duct lobular unit and duct were obtained from fibroadenoma and mastopathy patients. Neoplastic breast tissue samples included ductal carcinoma in situ (DCIS) (n=43) and invasive ductal carcinoma (IDC) (n=60), including the tubule-forming type (n=20). These specimens were investigated by CD205, p63, and CD10...
Source: Histology and Histopathology - September 17, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Microscopic study of chronic charcot arthropathy foot bones contributes to understanding pathogenesis - A preliminary report.
CONCLUSION: Chronic stage CA foot bones presented features of both healing and fragile bone. The compromised bone quality may be due to thin and fragmented trabecular structure and reduced cellularity. PMID: 31508805 [PubMed - as supplied by publisher] (Source: Histology and Histopathology)
Source: Histology and Histopathology - September 13, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Alendronate effect in esophagus, stomach and liver: an animal based pathological study.
Authors: Papamitsou T, Sotiriou S, Papakoulas A, Toskas A, Kamperis D, Karachrysafi S, Dietrich EM, Lialiaris S, Sioga A Abstract Bisphosphonates are commonly used in clinical practice. Their effectiveness is indisputable, however their adverse effects, especially in the GI tract, are still controversial. In our report, we demonstrate pathological findings of the effect of systematic alendronate administration in esophagus, stomach and the liver of an in-vivo animal model of 15 Wistar rats. Light microscopy with immunohistochemistry and electron microscopy were used. Microscopic findings of inflammation of the stom...
Source: Histology and Histopathology - September 11, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Gap junctions and expression of Cx36, Cx43 and Cx45 in the posterodorsal medial amygdala of adult rats.
Authors: Zancan M, Malysz T, Moura DJ, Morás AM, Steffens L, Rasia-Filho AA Abstract The posterodorsal medial amygdala (MePD) has an adapted synaptic organization that dynamically modulates reproduction and other social behaviors in rats. Discrete gap junctions between glial cells were previously reported in the MePD neuropil. Connexins (Cx) are components of gap junctions and indicative of cellular electrical coupling. Here, we report the ultrastructural occurrence of gap junctions between neurons in the MePD and demonstrate the expression and immunofluorescent labeling of Cx36, Cx43 and Cx45 in this subcor...
Source: Histology and Histopathology - September 10, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research

Innervation and nerve-immune cell contacts in mouse Peyer's patches.
Authors: Al-Shalan HAM, Hu D, Nicholls PK, Greene WK, Ma B Abstract Neural regulation of the function of the gastrointestinal tract (GIT) relies on a delicate balance of the two divisions of its nervous system, namely, the intrinsic and extrinsic divisions. The intrinsic innervation is provided by the enteric nervous system (ENS), whereas the extrinsic innervation includes sympathetic/parasympathetic nerve fibers and extrinsic sensory nerve fibers. In the present study, we used immunofluorescent staining of neurofilament-heavy (NF-H) to reveal the distribution of nerve fibers and their associations with immune cell...
Source: Histology and Histopathology - September 7, 2019 Category: Cytology Tags: Histol Histopathol Source Type: research