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Procedure: Transplants

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Total 526 results found since Jan 2013.

Review of Current Machine Perfusion Therapeutics for Organ Preservation
Because of the high demand of organs, the usage of marginal grafts has increased. These marginal organs have a higher risk of developing ischemia-reperfusion injury, which can lead to posttransplant complications. Ex situ machine perfusion (MP), compared with the traditional static cold storage, may better protect these organs from ischemia-reperfusion injury. In addition, MP can also act as a platform for dynamic administration of pharmacological agents or gene therapy to further improve transplant outcomes. Numerous therapeutic agents have been studied under both hypothermic (1–8°C) and normothermic settings. Here, we...
Source: Transplantation - August 26, 2020 Category: Transplant Surgery Tags: Reviews Source Type: research

The role of machine perfusion in liver xenotransplantation.
Abstract PURPOSE OF REVIEW: To review the role of machine perfusion in advancing the study and clinical application of liver xenotransplantation to liver transplantation. RECENT FINDINGS: Recent multicenter trial has shown the benefits of normothermic machine perfusion (NMP) in the assessment and selection of suitable allografts for liver transplantation, especially marginal liver allografts. Advances in ex-vivo therapeutic intervention with proof-of-concept studies demonstrating successful ex-vivo genetic modification of donor allografts and blockade of gene expression with siRNA. SUMMARY: Xenotransplan...
Source: Current Opinion in Organ Transplantation - August 20, 2020 Category: Transplant Surgery Authors: MacConmara M, Feizpour CA, Shubin A, Vagefi PA Tags: Curr Opin Organ Transplant Source Type: research

Epigenetic upregulation of HOXC10 in non-small lung cancer cells.
Abstract The homeobox genes (HOX) have emerged as a new family of master regulators of development and cancer. In the current study, we examined the expression and function of HOXC10 in human non-small cell lung cancer (NSCLC). We observed increased expression of HOXC10 in the more aggressive human NSCLC cell line NCI-H23 over the well differentiated A549 cells. To elucidate the expression and function of HOXC10 in NSCLC cells, we employed RT-PCR, immunoblotting, methylation-specific PCR, apoptosis assays, and xenograft model. Overexpression of HOXC10 in A549 cells conveyed increased proliferation, reduced apoptos...
Source: Aging - July 18, 2020 Category: Biomedical Science Authors: Li M, Alsager JS, Wang Z, Cheng L, Shan B Tags: Aging (Albany NY) Source Type: research

FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells
Conclusions: Our data therefore demonstrate the importance of FGF9 in the determination of SC fate via the FGF9-FGFR2-Akt pathway and reveal the therapeutic benefit of FGF9-NLCs.
Source: Theranostics - July 3, 2020 Category: Molecular Biology Authors: Chia-Wei Huang, Shih-Yu Lu, Tzu-Chieh Huang, Bu-Miim Huang, H. Sunny Sun, Shang-Hsun Yang, Jih-Ing Chuang, Yuan-Yu Hsueh, Yi-Ting Wu, Chia-Ching Wu Tags: Research Paper Source Type: research

Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis
Conclusion: Our studies for the first time demonstrated that the acetate produced from gut microbiota mediated the dysregulation of cholesterol homeostasis through the activation of GPR43, thereby contributing to the tubulointerstitial injury of DN, suggesting that gut microbiota reprogramming might be a new strategy for DN prevention and therapy.
Source: Theranostics - July 3, 2020 Category: Molecular Biology Authors: Ze Bo Hu, Jian Lu, Pei Pei Chen, Chen Chen Lu, Jia Xiu Zhang, Xue Qi Li, Ben Yin Yuan, Si Jia Huang, Xiong Zhong Ruan, Bi Cheng Liu, Kun Ling Ma Tags: Research Paper Source Type: research

GINS2 affects cell viability, cell apoptosis, and cell cycle progression of pancreatic cancer cells via MAPK/ERK pathway
Conclusions: GINS2 interference inhibited cell viability, induced cell cycle arrest, and promoted cell apoptosis of pancreatic cancer cell lines via the MAPK/ERK pathway, and our findings may be valuable for treating pancreatic cancer.
Source: Journal of Cancer - July 2, 2020 Category: Cancer & Oncology Authors: Miao Zhang, Saifei He, Xing Ma, Ying Ye, Guoyu Wang, Juhua Zhuang, Yanan Song, Wei Xia Tags: Research Paper Source Type: research

Bone marrow mesenchymal stem cells upregulate PI3K/AKT pathway and down-regulate NF- κB pathway by secreting glial cell-derived neurotrophic factors to regulate microglial polarization and alleviate deafferentation pain in rats.
In this study, we investigated whether DP could be alleviated by BMSCs and the underlying mechanism. In vitro study, microglia was stimulated by lipopolysaccharide and then co-cultured with BMSC, GDNF or siRNA GDNF-BMSC. In vivo study, BMSC or siRNA GDNF-BMSC was transplanted intramedullarily on the 21st day after DP surgery. The expression of inflammatory-related factors were detected by RT-PCR and ELISA, RT-PCR,flow cytometry and immunofluorescence staining were performed to detect the expression of microglial surface markers, and Western blot was used to detect the expression levels of p-NF-kb, pPI3K, and pAKT. The pain...
Source: Neurobiology of Disease - May 15, 2020 Category: Neurology Authors: Zhong Z, Chen A, Fa Z, Ding Z, Xiao L, Wu G, Wang Q, Zhang R Tags: Neurobiol Dis Source Type: research

Hepatic CEACAM1 expression indicates donor liver quality and prevents early transplantation injury
Although CEACAM1 (CC1) glycoprotein resides at the interface of immune liver injury and metabolic homeostasis, its role in orthotopic liver transplantation (OLT) remains elusive. We aimed to determine whether/how CEACAM1 signaling may affect hepatic ischemia-reperfusion injury (IRI) and OLT outcomes. In the mouse, donor liver CC1 null mutation augmented IRI-OLT (CC1-KO→WT) by enhancing ROS expression and HMGB1 translocation during cold storage, data supported by in vitro studies where hepatic flush from CC1-deficient livers enhanced macrophage activation in bone marrow–derived macrophage cultures. Although hepatic CC1 ...
Source: Journal of Clinical Investigation - April 21, 2020 Category: Biomedical Science Authors: Kojiro Nakamura, Shoichi Kageyama, Fady M. Kaldas, Hirofumi Hirao, Takahiro Ito, Kentaro Kadono, Kenneth J. Dery, Hidenobu Kojima, David W. Gjertson, Rebecca A. Sosa, Maciej Kujawski, Ronald W. Busuttil, Elaine F. Reed, Jerzy W. Kupiec-Weglinski Source Type: research

Normothermic Preservation of Liver - What Does the Future Hold?
Authors: Bral M, Shapiro AMJ Abstract Recent years have demonstrated a surging interest in normothermic ex situ liver perfusion, with iterative experimental and clinical studies establishing this technology as providing obvious advantages over static cold storage. In particular, the safe prolongation of liver graft preservation even up to 1 week opens up possibilities of 'on circuit' interventions, which may radically change the logistics and scope of liver transplant practice. Such approaches are rife with potential, and have yet to be fully explored. Possibilities may include, but are not limited to mitochondria...
Source: Advances in Experimental Medicine and Biology - April 15, 2020 Category: Research Tags: Adv Exp Med Biol Source Type: research

Inhibitory effect of siRNA-Annexin A7 on growth, migration, and invasion in BGC823 cells and gastric cancer xenograftsin nude mice.
CONCLUSION: Gastric cancer xenografts were established in nude mice with human gastric cancer BGC823 cells. The volume and weight of tumor were decreased after inhibition of Annexin A7 expression in BGC823 cells. Tumor cells were arranged sparsely after inhibition of Annexin A7 expression in BGC823 cells. The siRNA-Annexin A7 inhibits Annexin A7 expression in transplanted gastric cancer of nude mice, and influences the growth, migration, and invasion of tumors by down-regulating the expression of PCNA and MMP-2, as well as up-regulating the expression of p27. PMID: 32211092 [PubMed]
Source: International Journal of Clinical and Experimental Pathology - March 28, 2020 Category: Pathology Authors: Yuan HF, Li Y, Tan BB, Zhao Q, Fan LQ, An ZJ Tags: Int J Clin Exp Pathol Source Type: research

HLA-B influences integrin beta-1 expression and pancreatic cancer cell migration.
In this study, we examined the effect of HLA-B on pancreatic cancer cell migration. HLA-B siRNA transfection increased the migration of the S2-013 pancreatic cancer cells but, in contrast, reduced migration of the PANC-1 and MIA PaCa-2 pancreatic cancer cell lines. Integrin molecules have previously been implicated in the upregulation of pancreatic cancer cell migration, and knockdown of HLA-B in S2-013 cells heightened the expression of integrin beta 1 (ITGB1), but in the PANC-1 and MIA PaCa-2 cells HLA-B knockdown diminished ITGB1 expression. A transmembrane sequence in an S2-013 HLA-B heavy chain matches a correspon...
Source: Experimental Cell Research - March 15, 2020 Category: Cytology Authors: Sliker BH, Goetz BT, Barnes R, King H, Maurer HC, Olive KP, Solheim JC Tags: Exp Cell Res Source Type: research

Exosome-based targeted RNA delivery for immune tolerance induction in skin transplantation.
In conclusion, we constructed a new exosome-based targeted delivery system which could effectively induce the immune tolerance in transplantation. This article is protected by copyright. All rights reserved. PMID: 32170897 [PubMed - as supplied by publisher]
Source: Biomed Res - March 13, 2020 Category: Research Authors: Li C, Guo F, Wang X, Liu D, Wu B, Wang F, Chen W Tags: J Biomed Mater Res A Source Type: research

Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer
We examined the role of dihydrolipoamide dehydrogenase (DLD) in ferroptosis induction in head and neck cancer (HNC). The effects of cystine deprivation or sulfasalazine treatment and of DLD gene silencing/overexpression were tested on HNC cell lines and mouse tumor xenograft models. These effects were analyzed with regard to cell death, lipid reactive oxygen species (ROS) and mitochondrial iron production, mitochondrial membrane potential, mRNA/protein expression, and α-ketoglutarate dehydrogenase (KGDH)/succinate/aconitase activities. Cystine deprivation induced ferroptosis via glutaminolysis. Cystine deprivation or impo...
Source: Redox Biology - January 8, 2020 Category: Biology Source Type: research

BMP Antagonists Secreted by Mesenchymal Stromal Cells Improve Colonic Organoid Formation: Application for the Treatment of Radiation-induced Injury.
This study provides evidence of the potential of ECO to limit late radiation effects on the colon and opens perspectives on combined strategies to improve their amplification abilities and therapeutic effects. PMID: 33108903 [PubMed - in process]
Source: Cell Transplantation - January 1, 2020 Category: Cytology Authors: Moussa L, Lapière A, Squiban C, Demarquay C, Milliat F, Mathieu N Tags: Cell Transplant Source Type: research

Resveratrol Ameliorates the Malignant Progression of Pancreatic Cancer by Inhibiting Hypoxia-induced Pancreatic Stellate Cell Activation.
The objective of this study was to explore whether PSCs and hypoxia synergistically mediate aggressiveness in pancreatic cancer and detect the potential pleiotropic protective effects of resveratrol on hypoxia-induced pancreatic cancer progression. Human PSCs were treated with vehicle or resveratrol under normoxic or hypoxic conditions (3% O2), and PSC activation was assessed by immunofluorescence staining. SiRNA was used to silence hypoxia-inducible factor 1 (HIF-1) expression. The invasive capacity of Panc-1 and Mia Paca-2 cells cocultured with conditioned medium from PSCs was assessed by Transwell assays. To examine tum...
Source: Cell Transplantation - December 31, 2019 Category: Cytology Authors: Xiao Y, Qin T, Sun L, Qian W, Li J, Duan W, Lei J, Wang Z, Ma J, Li X, Ma Q, Xu Q Tags: Cell Transplant Source Type: research