Optimization and Comparison of Different Methods and Factors for Efficient Transformation of Brucella abortus RB51strain.
Abstract Background: The development of protective vaccines for Brucella spp. has been hampered by the difficulty in transformation of Brucella cells with foreign DNA for genetic manipulation. It seems that the formation of Brucella spheroplasts would increase the efficiency of transformation. The aim of this study was to devise an efficient method for the transformation of Brucella spp. Materials and Methods: At first, spheroplast of Brucella was prepared by glycine and ampicillin induction and transformed using optimized protocols of CaCl2, electroporation, and lipofection methods. Then, the efficacy of tra...
Source: Biomed Res - June 16, 2019 Category: Research Authors: Gheibi A, Khanahmad H, Kardar GA, Boshtam M, Rezaie S, Kazemi B, Khorramizadeh MR Tags: Adv Biomed Res Source Type: research

Study on the Potential Biomarkers of Maternal Urine Metabolomics for Fetus with Congenital Heart Diseases Based on Modified Gas Chromatograph-Mass Spectrometer.
Conclusion: The major potential biomarkers in maternal urine associated with CHD were 4-hydroxybenzeneacetic acid, 5-trimethylsilyloxy-n-valeric acid, propanedioic acid, hydracrylic acid, and uric acid, respectively. These results indicated that the short chain fatty acids (SCFAs) and aromatic amino acid metabolism may be relevant with CHD. PMID: 31198782 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 16, 2019 Category: Research Authors: Xie D, Luo Y, Xiong X, Lou M, Liu Z, Wang A, Xiong L, Kong F, Wang Y, Wang H Tags: Biomed Res Int Source Type: research

Clinical and Laboratory Characteristics of Symptomatic and Asymptomatic Neurosyphilis in HIV-Negative Patients: A Retrospective Study of 264 Cases.
Abstract A retrospective study was performed to compare the differences in clinical and laboratory features of asymptomatic neurosyphilis (ANS) and symptomatic neurosyphilis (SNS). A total of 264 HIV-negative inpatients with neurosyphilis were enrolled from Beijing Ditan Hospital and Beijing Tiantan Hospital between January 2014 and May 2018, including 110 SNS and 154 ANS. The SNS group had more patients in males, older median age and without antisyphilis treatment than ANS group (P
Source: Biomed Res - June 16, 2019 Category: Research Authors: Li W, Jiang M, Xu D, Kou C, Zhang L, Gao J, Qin K, Wu W, Zhang X Tags: Biomed Res Int Source Type: research

Motor Evoked Potentials after Supraspinal Stimulation in Pre- and Postoperative Evaluations of Patients with Cervical Radiculopathy.
Conclusions: Tests comparing MRI sensitivity to neurophysiological tests show that neuroimaging is more sensitive in diagnostics of patients with cervical radiculopathy; however, clinical neurophysiology tests are more specific in reference to clinical trials. PMID: 31198784 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 16, 2019 Category: Research Authors: Bryndal A, Wojtysiak M, Moskal J, Lipiec-Kowalska J, Borowczyk M, Tańska M, Grochulska A, Huber J, Majchrzycki M Tags: Biomed Res Int Source Type: research

Differences in Leukocyte Telomere Length between Coronary Heart Disease and Normal Population: A Multipopulation Meta-Analysis.
Abstract Coronary heart disease (CHD) is one of the most common causes of death in the world. Numerous studies have shown that as the degree of atherosclerotic disease increases, leukocyte telomere length gradually decreases. Short telomeres increase the risk of all-cause death and cardiovascular death. However, the reported results are not consistent, since the experimental design method, the measurement method, and the disease outcome are different. Therefore, we searched five major literature databases (Pubmed, Web of science, Embase, CNKI, and Wangfang) and finally included 18 eligible articles (including 5,15...
Source: Biomed Res - June 16, 2019 Category: Research Authors: Xu X, Hu H, Lin Y, Huang F, Ji H, Li Y, Lin S, Chen X, Duan S Tags: Biomed Res Int Source Type: research

Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers.
This study found that SSR marker was more informative compared to ISSR marker in determination of gene diversity, polymorphic information content (PIC), and heterozygosity in this population. SSR also revealed high ability in evaluating diversity levels, genetic structure, and relationships of torch ginger due to their codominance and rich allelic diversity. High level of genetic diversity discovered by SSR markers showed the effectiveness of this marker to detect the polymorphism in this germplasm collection. PMID: 31198786 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 16, 2019 Category: Research Authors: Ismail NA, Rafii MY, Mahmud TMM, Hanafi MM, Miah G Tags: Biomed Res Int Source Type: research

TROAP Promotes Breast Cancer Proliferation and Metastasis.
Abstract Trophinin-associated protein (TROAP) is a cytoplasmic protein required for microtubular cytoskeleton regulation and spindle assembly, and its expression plays a critical role in the initiation and progression of various types of cancer. However, little is known about the role of TROAP in breast cancer (BC). TROAP mRNA expression levels and clinical data from Gene Expression Omnibus (GEO) datasets (GSE42568, 104 BC patients; GSE1456, 159 BC patients; and GSE21653, 266 BC patients) were analyzed by the R2: Genomics Analysis and Visualization Platform to estimate overall survival (OS). We also analyzed the g...
Source: Biomed Res - June 16, 2019 Category: Research Authors: Li K, Zhang R, Wei M, Zhao L, Wang Y, Feng X, Yang Y, Yang S, Zhang L Tags: Biomed Res Int Source Type: research

Erratum.
ERRATUM. J Biomed Mater Res A. 2019 Aug;107(8):1860 Authors: PMID: 31199604 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 16, 2019 Category: Research Tags: J Biomed Mater Res A Source Type: research

Aspergillus flavus Promoted the Growth of Soybean and Sunflower Seedlings at Elevated Temperature.
Abstract The current climate changes in the form of global warming are one of the leading threats to agricultural crops (including soybean and sunflower). To enable the crops to cope with the heat stress, innovative steps are needed to be taken as soon as possible. Fungal endophytes are known to secrete secondary metabolites that promote the growth of host plants under stress conditions. Therefore, we have isolated endophytic fungus from Euphorbia indica (a wild desert plant) and tested it for plant growth promoting activities. The culture filtrate of the fungal strains exhibited the presence of secondary metaboli...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Ismail, Hamayun M, Hussain A, Afzal Khan S, Iqbal A, Lee IJ Tags: Biomed Res Int Source Type: research

Treatment-Related Prognostic Factors in Managing Osteosarcoma around the Knee with Limb Salvage Surgery: A Lesson from a Long-Term Follow-Up Study.
Abstract Purpose: The aim of this study was to assess the treatment-related factors associated with local recurrence and overall survival of patients with osteosarcoma treated with limb-salvage surgery. Patients and Methods: Treatment-related factors were analyzed to evaluate their effects on local recurrence-free survival (LRFS) and overall survival (OS) in 182 patients from 2004 to 2013. Results: The mean length of follow-up was 73.4 ± 34.7 months (median, 68 months; range, 12-173 months), and 63 patients died by the end of the follow-up. The 5-year and 10-year overall survival rates were 68.6 &...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Hu J, Zhang C, Zhu K, Zhang L, Cai T, Zhan T, Luo X Tags: Biomed Res Int Source Type: research

Evaluation of Metabolic Syndrome and Its Associated Risk Factors in Type 2 Diabetes: A Descriptive Cross-Sectional Study at the Komfo Anokye Teaching Hospital, Kumasi, Ghana.
Conclusions. The prevalence of metabolic syndrome observed among the study population was 90.6%, with a higher percentage in females than males. High triglyceride levels and high waist circumference were the main risk factors for MS in the diabetic population. PMID: 31187045 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 14, 2019 Category: Research Authors: Agyemang-Yeboah F, Eghan BAJ, Annani-Akollor ME, Togbe E, Donkor S, Oppong Afranie B Tags: Biomed Res Int Source Type: research

Milk Fat Globule Membrane Supplementation Promotes Neonatal Growth and Alleviates Inflammation in Low-Birth-Weight Mice Treated with Lipopolysaccharide.
Abstract Impaired intestinal mucosal integrity and immunity are frequently observed in low-birth-weight (LBW) animals, which lead to inadequate growth and high neonatal mortality. However, the mechanisms of intestinal dysfunction in LBW animals are still unclear. Milk fat globule membrane (MFGM), a protein-lipid complex surrounding the fat globules in milk, has many healthful benefits for animals. Therefore, this study was conducted to explore the effect of MFGM supplementation on intestinal injury and inflammation in LBW mouse pups while being challenged with lipopolysaccharide (LPS). C57BL/6J LBW female neonatal...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Huang S, Wu Z, Liu C, Han D, Feng C, Wang S, Wang J Tags: Biomed Res Int Source Type: research

Feasibility of T2 Mapping and Magnetic Transfer Ratio for Diagnosis of Intervertebral Disc Degeneration at the Cervicothoracic Junction: A Pilot Study.
Conclusion: We proved the better accuracy of T2 mapping over MTR imaging to quantitatively evaluate the intervertebral disc degeneration of the cervicothoracic junction. PMID: 31187047 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 14, 2019 Category: Research Authors: Zhang C, Lin Y, Han Z, Gao L, Guo R, Shi Q, Chen L, Chen C Tags: Biomed Res Int Source Type: research

Pharmacokinetics and Safety of Icotinib Hydrochloride Cream in Patients with Mild to Moderate Chronic Plaque Psoriasis: A Randomized Double-Blind Vehicle-Controlled Phase 1 Study.
Conclusion: This phase 1 study demonstrated favorable safety, tolerable toxicity, and preliminary efficacy of icotinib cream in patients with mild to moderate psoriasis. The dose concentration of 2.0% (twice daily based on the fingertip unit method) is recommended for further study. Study Design: This is a single-center, randomized, double-blind, and vehicle-controlled study. PMID: 31187048 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 14, 2019 Category: Research Authors: Liu L, Lou H, Zhou J, Shen Y, Zheng M, Ruan Z Tags: Biomed Res Int Source Type: research

Longitudinal Changes of PAI-1, MMP-2, and VEGF in Peritoneal Effluents and Their Associations with Peritoneal Small-Solute Transfer Rate in New Peritoneal Dialysis Patients.
Abstract Patients on peritoneal dialysis (PD) encounter peritoneal functional and structural alterations. It is still unknown whether levels of plasminogen activator inhibitor type 1 (PAI-1), matrix metalloproteinases- (MMP-) 2, and vascular endothelial growth factor (VEGF) exhibit dynamic changes in peritoneal effluents. The aim of the present study was to investigate the longitudinal changes in these biomarkers in PD patients and their association with peritoneal small-solute transfer rate (PSTR). This prospective, single-center cohort study included 70 new PD patients. The presence of PAI-1, MMP-2, and VEGF in ...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Hao N, Chiou TT, Wu CH, Lei YY, Liang PL, Chao MC, Yang H, Chen JB Tags: Biomed Res Int Source Type: research

Noninvasive Indirect Markers of Liver Fibrosis in Alcoholics.
Abstract The aim of this study was to evaluate the diagnostic values of noninvasive indirect markers of liver fibrosis: APRI, GAPRI, Forns, FIB-4, Age-Platelet, and Hepascore in alcoholics. Blood samples were collected from a randomized group of 142 alcohol-dependent patients. The diagnosis of dependency was made according to the ICD-10 WHO criteria. The values of noninvasive markers were calculated with specific algorithms. The fibrosis stage was evaluated on the basis of FibroTest. The values of APRI, Forns, FIB-4, GAPRI, AP, and Hepascore differ between various stages of liver fibrosis. Patients with fibrosis s...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Chrostek L, Przekop D, Gruszewska E, Gudowska-Sawczuk M, Cylwik B Tags: Biomed Res Int Source Type: research

Gadolinium Retention and Clearance in the Diabetic Brain after Administrations of Gadodiamide, Gadopentetate Dimeglumine, and Gadoterate Meglumine in a Rat Model.
Abstract Purpose: To evaluate gadolinium (Gd) retention and clearance in the brain of diabetic rats after administrations of gadodiamide, gadopentetate dimeglumine, and gadoterate meglumine. Materials and Methods: Both diabetic rats (n = 52) and normal rats (n = 52) intravenously received 20 injections of 0.6 mmol Gd/kg gadodiamide, gadopentetate dimeglumine, gadoterate meglumine, or saline. Both diabetic rats and normal rats were divided into 2 subgroups of 24 and 28 rats for the 7-day and 42-day evaluations (i.e., they were sacrificed at 7 days (n = 6 per group) and 42 days (n = 7 per group)), respectively,...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Wang ST, Hua ZX, Fan DX, Zhang X, Ren K Tags: Biomed Res Int Source Type: research

Analysis of Plasma EBV-DNA and Soluble Checkpoint Proteins in Nasopharyngeal Carcinoma Patients after Definitive Intensity-Modulated Radiotherapy.
Conclusions: This study demonstrated that the concentration of sPD-1 was significantly increased and EBV-DNA was significantly reduced in the NPC patients after IMRT. Plasma EBV-DNA level was a highly specific and sensitive biomarker for NPC diagnosis. Both sPD-1 expression and EBV-DNA concentration in plasma were related to the survival of patients. PMID: 31192256 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 14, 2019 Category: Research Authors: Ruan Y, Hu W, Li W, Lu H, Gu H, Zhang Y, Zhu C, Chen Q Tags: Biomed Res Int Source Type: research

Development and Validation of a Prediction Pneumothorax Model in CT-Guided Transthoracic Needle Biopsy for Solitary Pulmonary Nodule.
This study aimed to establish a predictive model for pneumothorax following CT-TNB for SPN. The prediction model was developed in a cohort that consisted of 311 patients with SPN who underwent CT-TNB. An independent external validation cohort contained 227 consecutive patients. The least absolute shrinkage and selection operator (Lasso) regression analysis was used for data dimension reduction and predictors selection. Multivariable logistic regression was used to develop the predictive model, which was presented with a nomogram. Area under the curve (AUC) was used to determine the discrimination of the proposed model. The...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Wang S, Tu J, Chen W Tags: Biomed Res Int Source Type: research

MMP2 and MMP10 Polymorphisms Are Related to Steroid-Induced Osteonecrosis of the Femoral Head among Chinese Han Population.
Conclusion: Our results verify that genetic polymorphisms of MMP2 and MMP10 contribute to steroid-induced ONFH susceptibility in the population of Chinese Han population, and our study provides new insights into the role that MMP2 and MMP10 plays in the mechanism of ONFH. PMID: 31192258 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 14, 2019 Category: Research Authors: Tian Y, An F, Wang J, Liu C, Wu H, Cao Y, Wang J, Wang G Tags: Biomed Res Int Source Type: research

Species, Risk Factors, and Antimicrobial Susceptibility Profiles of Bacterial Isolates from HIV-Infected Patients Suspected to Have Pneumonia in Mekelle Zone, Tigray, Northern Ethiopia.
This study estimated the prevalence of bacterial pneumonia in HIV patients, antimicrobial susceptibility patterns of pathogens implicated in pneumonia, and associated risk factors in Mekelle zone, Tigray, Northern Ethiopia, during August-December 2016. Methods: Sputum specimens were collected from 252 HIV seropositive individuals with suspected pneumonia. Data on sociodemographics and risk factors were also collected using a structured questionnaire. Blood, Chocolate, and Mac Conkey agar plates (Oxoid, Hampshire, UK) were used to grow the isolates. The isolated colonies were identified based on Gram stain, colony morp...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Adhanom G, Gebreegziabiher D, Weldu Y, Gebreyesus Wasihun A, Araya T, Legese H, Lopes BS, Saravanan M Tags: Biomed Res Int Source Type: research

Exosomes Derived from Human Bone Marrow Mesenchymal Stem Cells Stimulated by Deferoxamine Accelerate Cutaneous Wound Healing by Promoting Angiogenesis.
This study, aimed to investigate whether exosomes originated from bone marrow-derived MSCs (BMSCs) preconditioned by deferoxamine (DFO-Exos) exhibited superior proangiogenic property in wound repair and to explore the underlying mechanisms involved. Human umbilical vein endothelial cells (HUVECs) were used for assays involving cell proliferation, scratch wound healing, and tube formation. To test the effects in vivo, streptozotocin-induced diabetic rats were established. Two weeks after the procedure, histological analysis was used to measure wound-healing effects, and the neovascularization was evaluated as well. Our find...
Source: Biomed Res - June 14, 2019 Category: Research Authors: Ding J, Wang X, Chen B, Zhang J, Xu J Tags: Biomed Res Int Source Type: research

Cholesterol Degradation and Production of Extracellular Cholesterol Oxidase from Bacillus pumilus W1 and Serratia marcescens W8.
Abstract Cholesterol is a waxy substance present in all types of the body cells. The presence of higher concentration of low density lipoprotein (LDL) is characterized by abnormal cholesterol level and is associated with cardiovascular diseases which lead to the development of atheroma in arteries known as atherosclerosis. The transformation of cholesterol by bacterial cholesterol oxidase can provide a key solution for the treatment of diseases related to cholesterol and its oxidized derivatives. Previously isolated bacteria from oil-contaminated soil were screened for cholesterol degradation. Among fourteen, five...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Wali H, Rehman FU, Umar A, Ahmed S Tags: Biomed Res Int Source Type: research

Biomarkers of Community-Acquired Pneumonia: A Key to Disease Diagnosis and Management.
Abstract Community-acquired pneumonia (CAP) is a dangerous disease caused by a spectrum of bacterial and viral pathogens. The choice of specific therapy and the need for hospitalization or transfer to the intensive care unit are determined by the causative agent and disease severity. The microbiological analysis of sputum largely depends on the quality of the material obtained. The prediction of severity and the duration of therapy are determined individually, and existing prognostic scales are used generally. This review examines the possibilities of using specific serological biomarkers to detect the bacterial o...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Savvateeva EN, Rubina AY, Gryadunov DA Tags: Biomed Res Int Source Type: research

Bioinformatics Analysis of Gene Expression Profiles of Sex Differences in Ischemic Stroke.
In conclusion, the above ten genes may have a protective effect on IS females through their direct or indirect involvement in biological processes of immune response and apoptotic process, as well as in TNF and NOD-like receptor signaling pathways. The results of this study may help to gain new insights into the sex-specific mechanisms underlying IS females and may suggest potential therapeutic targets for disease treatment. PMID: 31183363 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Zhu W, Nan Y, Wang S, Liu W Tags: Biomed Res Int Source Type: research

Fourier-Based Footfall Placement Variability in Parkinson's Disease.
Abstract The current investigation examined whether Parkinson's patients (PD) have greater Fourier-based footfall placement gait with the greatest mobility dysfunction variability (FPV) than the age and gender matched control group and that variability would be the greatest in the PD participants with the greatest mobility dysfunction indexed Hoehn/Yahr scale. 35 persons undergoing PD and 30 age-matched controls participated in this investigation. Participants repeated two trials' normal walking and average and variability parameters of gait were measured using a 3.66 m electronic walkway. FPV was quantified as a ...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Shin S, Lim BO, Socie MJ, Sosonff JJ, Lee KK Tags: Biomed Res Int Source Type: research

Whole-Exome Sequencing Identified a Novel Compound Heterozygous Genotype in ASL in a Chinese Han Patient with Argininosuccinate Lyase Deficiency.
Abstract Pathogenic variants in the argininosuccinate lyase (ASL) gene have been shown to cause argininosuccinate lyase deficiency (ASLD); therefore, sequencing analysis offers advantages for prenatal testing and counseling in families afflicted with this condition. Here, we performed a genetic analysis of an ASLD patient and his family with an aim to offer available information for clinical diagnosis. The research subjects were a 23-month-old patient with a high plasma level of citrulline and his unaffected parents. Whole-exome sequencing identified potential related ASL gene mutations in this trio. Enzymatic act...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Zhao M, Hou L, Teng H, Li J, Wang J, Zhang K, Yang L Tags: Biomed Res Int Source Type: research

The Role of Ultrasound and Shear-Wave Elastography in Evaluation of Cervical Lymph Nodes.
Conclusion: A combination of epidemiologic and classic ultrasound parameters can discriminate between malignant and benign lymph nodes with satisfactory sensitivity and specificity. Examining the stiffness of lymph nodes by means of SWE does not add much new predictive power. PMID: 31183367 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Heřman J, Sedláčková Z, Fürst T, Vachutka J, Salzman R, Vomáčka J, Heřman M Tags: Biomed Res Int Source Type: research

The Empowerment of Adolescents with Type 1 Diabetes Is Associated with Their Executive Functions.
Conclusions: To conclude, we propose individualized diabetes education in this group of patients based on the assessment of readiness to change and executive functions. PMID: 31183368 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Łuczyński W, Łazarczyk I, Szlachcikowska I, Kiernozek Ż, Kaczmarek A, Szylaj O, Szadkowska A, Jarosz-Chobot P, Głowińska-Olszewska B, Bossowski A Tags: Biomed Res Int Source Type: research

Biomechanical Evaluation of Dual Plate Configurations for Femoral Shaft Fracture Fixation.
This study aimed at comparing the mechanical properties of conventional and locking dual plates in adjacent and orthogonal orientations for the surgical fixation of transverse femoral shaft fractures. It also assessed the failure mechanics after dual adjacent and orthogonal locking plate removal. Methods: Thirty-two composite femurs were transversally osteotomized and randomly assigned for fixation with either dual locking or compression plates in an adjacent or orthogonal configuration. Sixteen specimens were preloaded axially to 20 N and single-leg stance loads were simulated. The remaining sixteen constructs were s...
Source: Biomed Res - June 13, 2019 Category: Research Authors: El Beaino M, Morris RP, Lindsey RW, Gugala Z Tags: Biomed Res Int Source Type: research

Pinelliae Rhizoma Praeparatum Cum Alumine Extract: Sedative and Hypnotic Effects in Mice and Component Compounds.
Abstract Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) is useful for eliminating dampness and phlegm in clinical settings, targeting the main mechanisms of insomnia as defined in traditional Chinese medicine. However, little is known regarding the sedative and hypnotic effects of PRPCA. In the present study, we examined the sedative effects of PRPCA via a locomotor activity test and aimed to determine the most appropriate concentration of PRPCA for achieving these effects. The strongest sedative effects were observed at a PRPCA concentration of 0.45 g/ml. In addition, we investigated the hypnotic effect...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Lin S, Nie B, Song K, Ye R, Yuan Z Tags: Biomed Res Int Source Type: research

Aberrant Expression of Collagen Gene Family in the Brain Regions of Male Mice with Behavioral Psychopathologies Induced by Chronic Agonistic Interactions.
Abstract Chronic agonistic interactions promote the development of experimental psychopathologies in animals: a depression-like state in chronically defeated mice and the pathology of aggressive behavior in the mice with repeated wins. The abundant research data indicate that such psychopathological states are associated with significant molecular and cellular changes in the brain. This paper aims to study the influence of a 20-day period of agonistic interactions on the expression patterns of collagen family genes encoding the proteins which are basic components of extracellular matrix (ECM) in different brain re...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Smagin DA, Galyamina AG, Kovalenko IL, Babenko VN, Kudryavtseva NN Tags: Biomed Res Int Source Type: research

A Preliminary Study of Neonatal Cranial Venous System by Color Doppler.
Conclusion: The measurements percent of visualization of cerebral deep veins was higher than the percent of cerebral venous sinuses. The pulsation percent of measurement and the velocities of cerebral venous sinuses were absolutely higher than the cerebral deep venous system. The pairs of vascular blood flow velocities were nonsignificantly different from one another. PMID: 31183374 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Liu LY, Hong JL, Wu CJ Tags: Biomed Res Int Source Type: research

Exposure to Messages on Risk Factors for Noncommunicable Diseases in a Rural Province of Vietnam.
Conclusion: Intervention programs should focus on providing information primarily through television, considering influencing factors as well ensuring that messages reach target audiences. PMID: 31183375 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Pham BD, Kim BG, Nguyen TTH, Hoang VM Tags: Biomed Res Int Source Type: research

Development and Validation of Online Textual Pediatrician-Parent Communication Instrument Based on the SEGUE Framework.
This study aimed to develop and validate an OPPC instrument to provide measurement and quality characteristics for quality assessment and management. 490 OPPC exchanges of 61 tertiary hospitals from 9 Chinese provinces were obtained from the Spring Rain Doctor website. The SEGUE framework, OPPC feature, and a pilot study were considered to establish the instrument. An empirical study was conducted to validate it and the incidence of OPPC items was also analyzed. As a result, a four-dimensional, 15-item OPPC instrument was developed. The empirical results are as follows. Cronbach's α values of dimensions were 0.80, 0....
Source: Biomed Res - June 13, 2019 Category: Research Authors: Xiong Y, Wang D, Chen H, Wang X, Zhang X Tags: Biomed Res Int Source Type: research

Optimization and Standardization of Thermal Treatment as a Plasma Prefractionation Method for Proteomic Analysis.
This study aimed to optimize thermal treatment as a reliable prefractionation method and to compare it with two commercial kits, including HSA and IgG immunodepletion (IMDP) and combinatorial peptide ligand libraries (CPLL), using untreated plasma as a control condition. By varying the temperature and the incubation period, the optimal condition was found as treatment at 95°C for 20 min, which maintained about 1% recovery yield of soluble proteins. Consistency and reproducibility of thermal treatment-derived plasma subproteome were checked by two-dimensional electrophoresis. The coefficient of variation regarding prote...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Chiangjong W, Changtong C, Panachan J, Weeraphan C, Srisomsap C, Hongeng S, Svasti J, Chutipongtanate S Tags: Biomed Res Int Source Type: research

Prevalence of Metabolic Syndrome and Its Components in Bamboutos Division's Adults, West Region of Cameroon.
This study aimed to evaluate the prevalence of MetS, its individual components, and associated risk factors among Bamboutos Division's adults population using a Joint Interim Statement of the International Diabetes Federation (IDF) Task Force on Epidemiology and Prevention definitions parameters. A cross-sectional study was conducted from May 2016 to May 2018 in Mbouda ADLUCEM Hospital and Mbouda District Hospital, two reference hospitals in Bamboutos Division, west region of Cameroon. Interview, physical and clinical examinations, and lipid and fasting blood glucose measurements were conducted for 604 adults. The definiti...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Marbou WJT, Kuete V Tags: Biomed Res Int Source Type: research

Noninvasive Evaluation of the Pathologic Grade of Hepatocellular Carcinoma Using MCF-3DCNN: A Pilot Study.
Conclusions: This study indicates that MCF-3DCNN can be a promising technology for evaluating the pathologic grade of HCC based on DCE-MR images. PMID: 31183380 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 13, 2019 Category: Research Authors: Yang DW, Jia XB, Xiao YJ, Wang XP, Wang ZC, Yang ZH Tags: Biomed Res Int Source Type: research

Internal Biomechanical Study of a 70-Year-Old Female Human Lumbar Bi-Segment Finite Element Model and Comparison with a Middle-Aged Male Model.
In this study, the L3-L5 lumbar bi-segmental finite element model for elderly female was obtained from the Advanced Human Modeling Laboratory of the Bioengineering Center at Wayne State University. The effects of flexion and extension on bone geometry, distribution of ligament fibers, location of nucleus, and changes in intervertebral disc height were studied by comparing the results obtained before and after the update of older female and middle-aged male models. For the purpose of comparing the calculated range of motion (ROM) with the experimental data, additional calculations for axial rotation and lateral bending were...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Wu H, Peng J, Jin X Tags: Biomed Res Int Source Type: research

Evaluation of Anterior-Posterior Spine Curvatures and Incidence of Sagittal Defects in Children and Adolescents Practicing Traditional Karate.
Abstract Background and Study Aim: To evaluate anterior-posterior spine curvatures and incidence of sagittal defects in children and adolescents practicing traditional Karate. Material and Methods: 152 people aged 6-16 yrs, mean age 10.5±3.03. The study group (SG), subjects attending traditional Karate classes for min one year, 60 minutes twice a week (76 people). The control group (CG) randomly selected on 1:1 basis to match SG. The anterior-posterior curvatures were measured with a gravitational inclinometer. Sauder's norms were used to assess postural defects. Body mass, height, and BMI were measure...
Source: Biomed Res - June 13, 2019 Category: Research Authors: Katarzyna WC, Ewa S, Agnieszka G, Mirosław M, Marta N, Paweł O, Iwona TB Tags: Biomed Res Int Source Type: research

Gender-Related Effect of Sodium Dichloroacetate on the Number of Hassall's Corpuscles and RNA NKCC1 Expression in Rat Thymus.
tis D Abstract The aim was to investigate the effect of dichloroacetate (DCA) on thymus weight, Hassall's corpuscle number (HCs), and NKCC1 RNA expression in Wistar rats aged 4-5 weeks. They were investigated in the controls and DCA-treated gonad-intact and castrated males and females. The treatment lasted 4 weeks with DCA 200 mg/kg/day. At the end of the experiment, rat thymus was weighted, and its lobe was taken for the expression of NKCC1 RNA determined by the PCR method and of Hassall's corpuscles by immunohistochemistry. DCA caused a thymus weight decrease in DCA-treated gonad-intact rats of both genders as c...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Stanevičiūtė J, Juknevičienė M, Balnytė I, Valančiūtė A, Lesauskaitė V, Fadejeva J, Stakauskas R, Stakišaitis D Tags: Biomed Res Int Source Type: research

Demographic and Lifestyle Predictors of Prehypertension: A Cross-Sectional Study among Apparently Healthy Adults in Kumasi, Ghana.
Conclusion: The prevalence of prehypertension is high among apparently healthy Ghanaian adults (49.0%). Lower educational level, sedentary lifestyle, and alcohol consumption are the predominant risk factors for prehypertension in Kumasi. PMID: 31179316 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 11, 2019 Category: Research Authors: Owiredu EW, Dontoh E, Essuman SES, Bazanfara BB Tags: Biomed Res Int Source Type: research

N-Methyl-N-Nitrosourea-Induced Photoreceptor Degeneration Is Inhibited by Nicotinamide via the Blockade of Upstream Events before the Phosphorylation of Signalling Proteins.
Abstract N-methyl-N-nitrosourea (MNU), a known carcinogen, is generally used in animal models to chemically induce photoreceptor degeneration. It has been reported that nicotinamide (NAM) exerts a protective effect on MNU-induced photoreceptor degeneration. We investigated the molecular mechanisms on MNU-induced photoreceptor degeneration. Intraperitoneal MNU injection (75 mg/kg) in rats induced selective photoreceptor degeneration in 7 days. NAM administration completely inhibited photoreceptor degeneration. Photoreceptor layer abnormality was observed within 6 hours after MNU injection, whereas it was restored i...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Sugano E, Tabata K, Takezawa T, Shiraiwa R, Muraoka H, Metoki T, Kudo A, Iwama Y, Nakazawa M, Tomita H Tags: Biomed Res Int Source Type: research

Evaluation and Prediction of Mass Transport Properties for Porous Implant with Different Unit Cells: A Numerical Study.
This study would provide great helps for unit cell selection and biological performance optimization for 3D printed bone implants. PMID: 31179318 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 11, 2019 Category: Research Authors: Li J, Chen D, Fan Y Tags: Biomed Res Int Source Type: research

Examination of the Effects of Curvature in Geometrical Space on Accuracy of Scaling Derived Projections of Plant Biomass Units: Applications to the Assessment of Average Leaf Biomass in Eelgrass Shoots.
os-López A Abstract Conservation of eelgrass relies on transplants and evaluation of success depends on nondestructive measurements of average leaf biomass in shoots among other variables. Allometric proxies offer a convenient way to assessments. Identifying surrogates via log transformation and linear regression can set biased results. Views conceive this approach to be meaningful, asserting that curvature in geometrical space explains bias. Inappropriateness of correction factor of retransformation bias could also explain inconsistencies. Accounting for nonlinearity of the log transformed response relied ...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Echavarría-Heras H, Leal-Ramírez C, Villa-Diharce E, Montesinos-López A Tags: Biomed Res Int Source Type: research

Antimicrobial Activity of 4-Chlorocinnamic Acid Derivatives.
In this study, a collection of structurally related 4-chlorocinnamic acid esters was prepared using Fischer esterification reactions, alkyl or aryl halide esterification, and Mitsunobu and Steglich reactions. All of the esters were submitted to antimicrobial tests against strains of the species Candida albicans, Candida glabrata, Candida krusei, Candida guilliermondii, Pseudomonas aeruginosa, and Staphylococcus aureus. The compounds also were subjected to molecular docking study with the enzyme 14α-demethylase. Twelve esters derived from 4-chlorocinnamic acid were obtained, with yields varying from 26.3% to 97.6%, th...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Silva RHN, Andrade ACM, Nóbrega DF, de Castro RD, Pessôa HLF, Rani N, de Sousa DP Tags: Biomed Res Int Source Type: research

Quantitative Proteomic Analysis Reveals the Deregulation of Nicotinamide Adenine Dinucleotide Metabolism and CD38 in Inflammatory Bowel Disease.
Abstract Inflammatory bowel disease (IBD) has become a major health challenge worldwide. However, the precise etiological and pathophysiological factors involved in IBD remain unclear. Proteomics can be used for large-scale protein identification analysis. In the current study, using tandem mass tag- (TMT-) based shotgun proteomics, proteomic differences between intestinal tissue from health controls, patients with Crohn's disease (CD), and patients with ulcerative colitis (UC) were compared. Proteins with fold change>2 or
Source: Biomed Res - June 11, 2019 Category: Research Authors: Ning L, Shan G, Sun Z, Zhang F, Xu C, Lou X, Li S, Du H, Chen H, Xu G Tags: Biomed Res Int Source Type: research

Novel Hybrid Gels Made of High and Low Molecular Weight Hyaluronic Acid Induce Proliferation and Reduce Inflammation in an Osteoarthritis In Vitro Model Based on Human Synoviocytes and Chondrocytes.
Abstract High molecular weight hyaluronan (H-HA) has a pivotal role in the maintenance of normal functions of synovial fluid and structure of the articular joint, but it has been shown that its concentration is reduced in patients affected by degenerative cartilage diseases, such as osteoarthritis (OA). The aim of this study was to investigate the anti-inflammatory effects and properties of hybrid cooperative complexes based on high and low molecular weight hyaluronan (HCC) compared to H-HA on human primary cells derived by pathological joints. In addition, the rheological behavior of HCC was evaluated in order to...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Stellavato A, Vassallo V, La Gatta A, Pirozzi AVA, De Rosa M, Balato G, D'Addona A, Tirino V, Ruosi C, Schiraldi C Tags: Biomed Res Int Source Type: research

Eradication of HCV Infection with the Direct-Acting Antiviral Therapy in Renal Allograft Recipients.
Abstract Hepatitis C virus (HCV) infection unfavorably affects the survival of both renal patients undergoing hemodialysis and renal transplant recipients. In this subset of patients, the effectiveness and safety of different combinations of interferon-free direct-acting antiviral agents (DAAs) have been analyzed in several small studies. Despite fragmentary, the available data demonstrate that DAA treatment is safe and effective in eradicating HCV infection, with a sustained virologic response (SVR) rates nearly 95% and without an increased risk of allograft rejection. This review article analyzes the results of ...
Source: Biomed Res - June 11, 2019 Category: Research Authors: Armando C, Evangelista S, Massimiliano C, Silvia A, Gaia P, Paola I, Maria C, Gianluca M, Nicola C, Concetta Anna D, Massimo C, Caterina S Tags: Biomed Res Int Source Type: research

Intimate Partner Violence among Pregnant Women and Postpartum Depression in Vietnam: A Longitudinal Study.
Conclusions: The results showed strong and statistically significant associations between partner violence and postpartum depression. These findings clearly demonstrate a crucial need for relevant health professionals to identify women who are exposed to partner violence and screen for postpartum depression in order to mitigate the negative mental health outcomes among Vietnamese women. PMID: 31179324 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - June 11, 2019 Category: Research Authors: Tho Nhi T, Hanh NTT, Hinh ND, Toan NV, Gammeltoft T, Rasch V, Meyrowitsch DW Tags: Biomed Res Int Source Type: research