Virulence-Associated Genes and Antimicrobial Resistance of Aeromonas hydrophila Isolates from Animal, Food, and Human Sources in Brazil.
In this study, 110 selected Aeromonas hydrophila isolates isolated from food, animals, and human clinical material from 2010 to 2015 were analyzed. Antimicrobial susceptibility testing was performed by the disk diffusion method, and polymerase chain reaction was conducted to investigate the virulence genes hemolysin (hlyA), cytotoxic enterotoxin (act), heat-labile cytotonic enterotoxin (alt), aerolysin (aerA), and DNase-nuclease (exu). At least 92.7% of the isolates had one of the investigated virulence genes. Twenty different virulence profiles among the isolates were recognized, and the five investigated virulence genes ...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Roges EM, Gonçalves VD, Cardoso MD, Festivo ML, Siciliano S, Berto LH, Pereira VLA, Rodrigues DDP, de Aquino MHC Tags: Biomed Res Int Source Type: research

Isorhamnetin Induces Melanoma Cell Apoptosis via the PI3K/Akt and NF- κB Pathways.
Isorhamnetin Induces Melanoma Cell Apoptosis via the PI3K/Akt and NF-κB Pathways. Biomed Res Int. 2020;2020:1057943 Authors: Duan R, Liang X, Chai B, Zhou Y, Du H, Suo Y, Chen Z, Li Q, Huang X Abstract Malignant melanoma is characterized by its bad prognosis for aggressiveness, drug resistance, and early metastasis. Isorhamnetin (3'-methoxy-3,4',5,7-tetrahydroxyflavone; IH) is a natural flavonoid that has been investigated for its antitumor effects in breast cancer, colon cancer, and gastric cancer through inducing cell apoptosis. Given its role in tumor inhibition, no research has been conducte...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Duan R, Liang X, Chai B, Zhou Y, Du H, Suo Y, Chen Z, Li Q, Huang X Tags: Biomed Res Int Source Type: research

The Impact of Intraoperative Hypothermia on Blood Loss and Allogenic Blood Transfusion in Total Knee and Hip Arthroplasty: A Retrospective Study.
Abstract Background: Total joint arthroplasty (TJA) usually leads to substantial blood loss, which may cause allogenic blood transfusion. Hypothermia occurring during operation has been reported to increase blood loss and transfusion rates in nonorthopedic cohorts. However, the relationship between intraoperative hypothermia and blood loss remains controversial in patients undergoing orthopedic surgeries. The aims of this study were to investigate the incidence of hypothermia and identify the impact of intraoperative body temperature and hypothermia on blood loss and transfusion rates in total knee and hip arthrop...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Pan P, Song K, Yao Y, Jiang T, Jiang Q Tags: Biomed Res Int Source Type: research

Sorafenib-Loaded Long-Circulating Nanoliposomes for Liver Cancer Therapy.
Abstract A type of sorafenib- (SOR-) loaded long-circulating nanoliposome was constructed, and the targeting performance and antitumor effects of the prepared liposome were evaluated in the present study. Polyethylene glycol- (PEG-) modified long-circulating nanoliposomes (LC-NPs) were designed and prepared using reverse evaporation, and the LC-NPs were used for delivering sorafenib (LC-PEG-SOR-NPs). Then, the anti-VEGFR antibody as a targeting moiety was chemically coupled with LC-PEG-SOR-NPs to form liver cancer-targeted nanoliposomes (anti-VEGFR-LC-PEG-SOR-NPs). The drug entrapment and loading efficiency were m...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Ye H, Zhou L, Jin H, Chen Y, Cheng D, Jiang Y Tags: Biomed Res Int Source Type: research

The Predictive Role of PIK3CA Mutation Status on PI3K Inhibitors in HR+ Breast Cancer Therapy: A Systematic Review and Meta-Analysis.
Conclusion: In this meta-analysis, it suggests that the association between clinical outcomes of PI3K inhibitors and PIK3CA mutation status is dramatic. PIK3CA mutations were a favorable factor in the clinical outcomes of HR+ breast cancer treated with PI3K inhibitors. PMID: 32461963 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Wang M, Li J, Huang J, Luo M Tags: Biomed Res Int Source Type: research

Serum Antibody and Glomerular Antigen of Antiphospholipase A2 Receptor in Chinese Patients with Idiopathic Membranous Nephropathy.
Abstract Background: M-type phospholipase A2 receptor (PLA2R) is the first autoantigen responsible for idiopathic membranous nephropathy (IMN). However, serum PLA2R antibody (PLA2R-Ab) can be inaccurate in distinguishing between IMN and secondary membranous nephropathy, while renal PLA2R antigen (PLA2R-Ag) emerges as an ancillary diagnostic. The present study is aimed at examining the associations between PLA2R-Ab in sera and PLA2R-Ag in kidneys in IMN patients. Methods: A total of 93 patients with IMN were retrospectively identified. Their serum PLA2R-Ab and renal PLA2R-Ag expression levels were determined, ...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhang QH, Wu M, Hu ZG, Liu XB, Huang B, Zhang Y, Liu B, Zhu YS, Wang L, Sun ZX Tags: Biomed Res Int Source Type: research

The SLC Family Are Candidate Diagnostic and Prognostic Biomarkers in Clear Cell Renal Cell Carcinoma.
This study suggests that SLC22A6, SLC22A7, SLC22A13, SLC25A4, SLC34A1, and SLC44A4 may be potential targets for the clinical diagnosis, prognosis, and treatment of ccRCC patients. PMID: 32461965 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Kang W, Zhang M, Wang Q, Gu D, Huang Z, Wang H, Xiang Y, Xia Q, Cui Z, Jin X Tags: Biomed Res Int Source Type: research

Clinical and Biological Correlates of Preoperative Cognitive Functioning of Glioma and Meningioma Patients.
This study aimed to investigate the association of high-sensitivity C-reactive protein (hsCRP) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) serum concentrations with cognitive functions of glioma and meningioma patients. Methods: 177 brain tumor patients awaiting for brain tumor surgery participated in the study. Patients were assessed preoperatively, using neuropsychological tests for verbal memory, psychomotor speed, mental flexibility, and verbal fluency. The functional status of patients was evaluated using the Karnofsky Performance Index. Blood samples were drawn for evaluation of serum hsCRP and NT-...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Pranckeviciene A, Deltuva VP, Tamasauskas A, Zegliene J, Bunevicius A Tags: Biomed Res Int Source Type: research

Positive Microbiological Findings at the Site of Presumed Aseptic Revision Arthroplasty Surgery of the Hip and Knee Joint: Is a Surgical Revision Always Necessary?
Abstract Little is known about patients that undergo presumed aseptic revision arthroplasty surgery of the hip and knee joint and having positive microbiological findings of the intraoperatively taken tissue samples. 228 "aseptic" operations were retrospectively analyzed from prospectively collected data with regard to the following parameters: demographic data; reasons for primary and revision surgery, respectively; time between primary and revision surgery; preoperative laboratory findings; microbiological and histopathological findings; type and length of systemic antibiotic therapy; clinical outcome;...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Anagnostakos K, Thiery A, Meyer C, Sahan I Tags: Biomed Res Int Source Type: research

A Multicenter Study Evaluating the Stages of Change in Food Consumption with Warning Labels among Chilean University Students.
lería JM Abstract Objective: To analyze the stage of change in food consumption with warning labels among Chilean university students. Materials and Methods: Cross-sectional study which applied surveys in universities from all over the country. Study included 4807 participants of 18 to 40 years of both sexes who were asked about the level of knowledge of the new food law and food consumption with warning signals, including questions regarding their willingness to behavior change according to Prochaska's transtheoretical model. To compare continuous variables, Student's t-test was used in the statistica...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Durán Agüero S, Araneda J, Ahumada D, Silva Rojas J, Bonacich RB, Caichac A, Salamanca MF, Villarroel P, Fernandez E, Pacheco V, Martinovic PA, Wilson W, Neira AM, Encina C, Tillería JM Tags: Biomed Res Int Source Type: research

Novel Discoveries Targeting Pathogenic Gut Microbes and New Therapies in Pancreatic Cancer: Does Pathogenic E. coli Infection Cause Pancreatic Cancer Progression Modulated by TUBB/Rho/ROCK Signaling Pathway? A Bioinformatic Analysis.
This study is aimed at obtaining the pathway in the microbial tumorigenesis of PC. Microarray datasets GSE27890, GSE46234, and GSE17610 were downloaded from the GEO (Gene Expression Omnibus) database. Differential analysis was performed for every single gene chip using the R software package ("Limma" package), and functional enrichment analyses were carried out by DAVID (Database for Annotation, Visualization and Integrated Discovery). The PPI (protein-protein interaction) network was constructed with the Search Tool for the Retrieval of Interacting Genes (STRING). The survival analysis was performed by GEPIA and...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Luo W, Cao Z, Qiu J, Liu Y, Zheng L, Zhang T Tags: Biomed Res Int Source Type: research

Intracranial Infections in Neurological Surgery: The Changes of Circular RNA Expression and Their Possible Function Mechanism.
Conclusions: hsa_circRNA_402632, hsa_circRNA_008636, and hsa_circRNA_405481 may be potential diagnostic markers for central nervous system infection after neurological surgery. PMID: 32461970 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Niu J, Xu H, Yang B Tags: Biomed Res Int Source Type: research

The Cataleptic, Asymmetric, Analgesic, and Brain Biochemical Effects of Parkinson's Disease Can Be Affected by Toxoplasma gondii Infection.
Conclusions: The current study indicates that TG infection could improve the cataleptic, asymmetric, nociceptive and behaviors; the level of striatal dopamine release; BDNF levels; TAC; and MDA in PD rats. PMID: 32461971 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Taherianfard M, Riyahi M, Razavi M, Bavandi Z, Eskandari Roozbahani N, Namavari MM Tags: Biomed Res Int Source Type: research

Tetrandrine Attenuated Doxorubicin-Induced Acute Cardiac Injury in Mice.
In conclusion, our study found that tetrandrine could improve cardiac function and prevent the development of DOX-related cardiac injury through activation of Nrf2. PMID: 32461972 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Li G, Li WR, Jin YG, Jie QQ, Wang CY, Wu L Tags: Biomed Res Int Source Type: research

Design of a Multiepitope-Based Peptide Vaccine against the E Protein of Human COVID-19: An Immunoinformatics Approach.
Conclusion: The T-cell epitope-based peptide vaccine was designed for COVID-19 using the envelope protein as an immunogenic target. Nevertheless, the proposed vaccine rapidly needs to be validated clinically in order to ensure its safety and immunogenic profile to help stop this epidemic before it leads to devastating global outbreaks. PMID: 32461973 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Abdelmageed MI, Abdelmoneim AH, Mustafa MI, Elfadol NM, Murshed NS, Shantier SW, Makhawi AM Tags: Biomed Res Int Source Type: research

Flavonoids in Ageratum conyzoides L. Exert Potent Antitumor Effects on Human Cervical Adenocarcinoma HeLa Cells In Vitro and In Vivo.
In conclusion, our results demonstrated the obvious antitumor effects of flavonoids in A. conyzoides on HeLa cells, suggesting that flavonoids in A. conyzoides could be provided as a novel therapeutic compound for human cervical adenocarcinoma. PMID: 32461974 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Lin Z, Lin Y, Shen J, Jiang M, Hou Y Tags: Biomed Res Int Source Type: research

Alphavirus Replication: The Role of Cardiac Glycosides and Ion Concentration in Host Cells.
NP, Burth P Abstract Alphaviruses are arthropod-borne viruses that can cause fever, rash, arthralgias, and encephalitis. The mosquito species Aedes aegypti and Aedes albopictus are the most frequent transmitters of alphaviruses. There are no effective vaccines or specific antivirals available for the treatment of alphavirus-related infections. Interestingly, changes in ion concentration in host cells have been characterized as critical regulators of the alphavirus life cycle, including fusion with the host cell, glycoprotein trafficking, genome translation, and viral budding. Cardiac glycosides, which are classica...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Souza-Souza KFC, Gonçalves-de-Albuquerque CF, Cirne-Santos C, Paixão ICNP, Burth P Tags: Biomed Res Int Source Type: research

Low-Frequency Repetitive Transcranial Magnetic Stimulation for the Treatment of Chronic Tinnitus: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
Conclusion: Low-frequency rTMS is well tolerated but not effective in treating chronic tinnitus based on the current analysis of pooled data. Further studies with modified and uniform protocols are required to investigate the potential benefit of rTMS in chronic tinnitus. PMID: 32461976 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Dong C, Chen C, Wang T, Gao C, Wang Y, Guan X, Dong X Tags: Biomed Res Int Source Type: research

Efficacy of Neoadjuvant Chemotherapy with Epirubicin and Cyclophosphamide and Weekly Paclitaxel and Trastuzumab in Human Epidermal Growth Factor Receptor 2-Positive Breast Carcinoma: A Real-World Study.
Conclusions: HER2+ BC patients showed improvement in pCR and DFS after neoadjuvant trastuzumab treatment. Patients without pCR had prolonged DFS after trastuzumab maintenance. Although the prognosis of luminal B/HER2+ BC showed favorable outcomes in the non-pCR subgroup, those receiving trastuzumab showed no survival advantage. PMID: 32461977 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhang M, Li L, Zhang S, Zhu W, Yang S, Di G, Ma X, Yang H Tags: Biomed Res Int Source Type: research

α 1-Adrenergic Receptor Blockade by Prazosin Synergistically Stabilizes Rat Peritoneal Mast Cells.
Conclusions: This study provided electrophysiological evidence for the first time that adrenaline dose-dependently inhibited the process of exocytosis, confirming its usefulness as a potent mast cell stabilizer. The pharmacological blockade of α 1-adrenergic receptor by prazosin synergistically potentiated such mast cell-stabilizing property of adrenaline, which is primarily mediated by β 2-adrenergic receptors. PMID: 32461978 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Abe N, Toyama H, Ejima Y, Saito K, Tamada T, Yamauchi M, Kazama I Tags: Biomed Res Int Source Type: research

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro.
Abstract It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three in vitro models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 34...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Segan S, Jakobi M, Khokhani P, Klimosch S, Billing F, Schneider M, Martin D, Metzger U, Biesemeier A, Xiong X, Mukherjee A, Steuer H, Keller BM, Joos T, Schmolz M, Rothbauer U, Hartmann H, Burkhardt C, Lorenz G, Schneiderhan-Marra N, Shipp C Tags: Biomed Res Int Source Type: research

Ten-Year Surgical Outcomes and Prognostic Factors for French-Door Laminoplasty in the Treatment of Multilevel Cervical Spondylotic Myelopathy.
Abstract Objective: To analyze the ten-year surgical outcomes and postoperative complications of French-Door laminoplasty (FDL) in the management of multilevel cervical spondylotic myelopathy (MCSM) and analyze the prognostic factors for FDL in treating MCSM. Methods: 64 patients with MCSM, who were operated by FDL, were included in this study and followed up for at least 10 years. Clinical assessments including modified Japanese Orthopaedic Association (mJOA) score, age at surgery, preoperative symptom duration, operative time, blood loss and postoperative complications, radiological assessments including Co...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Chen G, Liu X, Chen N, Chen B, Zou X, Wei F, Liu S Tags: Biomed Res Int Source Type: research

Analysis of the Transmissibility Change of 2019-Novel Coronavirus Pneumonia and Its Potential Factors in China from 2019 to 2020.
Conclusion: The current prevention and control measures have effectively reduced the transmissibility of COVID-19; however, R 0t is still larger than the threshold 1. The results show that the government adopting the Wuhan lockdown strategy plays an important role in restricting the potential infected persons in Wuhan outflow from the epidemic area and avoiding a nationwide spread by quickly controlling the potential infection in Wuhan. Meanwhile, since Jan. 18, 2020, the people successively accessed COVID-19-related information via the Internet, which may help to effectively implement the government's prevention and contr...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhao Y, Wang R, Li J, Zhang Y, Yang H, Zhao Y Tags: Biomed Res Int Source Type: research

Hematological Indexes Can Be Used to Predict the Incidence of Hypothyroidism in Nasopharyngeal Carcinoma Patients after Radiotherapy.
Conclusion: A model combining TPO-Ab, TG-Ab, TG, and Fb can be used to screen populations at a high risk of developing hypothyroidism after radiotherapy. PMID: 32461982 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhou L, Chen J, Tao CJ, Huang S, Zhang J, Shen W, Zhu CN, Chen M, Yu ZH, Chen YY Tags: Biomed Res Int Source Type: research

Tetanus Toxoid Vaccination Uptake and Associated Factors among Mothers Who Gave Birth in the Last 12 Months in Errer District, Somali Regional State, Eastern Ethiopia.
Abstract Background: Maternal tetanus is defined as tetanus acquired during pregnancy or within 6 weeks after the end of conception. As tetanus is a vaccine-preventable disease, immunization of pregnant mothers with a TT (tetanus toxoid) dose is one of the most effective ways to protect against the disease. Some studies showed that 94% of neonatal mortality reduction could be achieved through immunization of pregnant and childbearing-age mothers with at least two doses of TT vaccination. Objective: To assess the uptake of tetanus toxoid vaccine and associated factors among mothers who gave birth in the last 1...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Gebremedhin TS, Welay FT, Mengesha MB, Assefa NE, Werid WM Tags: Biomed Res Int Source Type: research

Wealth Status, Health Insurance, and Maternal Health Care Utilization in Africa: Evidence from Gabon.
Conclusion: Women enrollment in health insurance and improved household's financial status can improve key maternal health services utilization. PMID: 32461984 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Sanogo NA, Yaya S Tags: Biomed Res Int Source Type: research

The Effects of Age and Cell Isolation on Collagen II Synthesis by Articular Chondrocytes: Evidence for Transcriptional and Posttranscriptional Regulation.
Abstract Adult articular cartilage synthesises very little type II collagen in comparison to young cartilage. The age-related difference in collagen II synthesis is poorly understood. This is the first systematic investigation of age-related differences in extracellular matrix synthesis in fresh articular cartilage and following isolation of chondrocytes. A histological comparison of 3-year-old skeletally mature and 6-month-old juvenile porcine cartilage was made. Differences in collagen II, aggrecan, and Sox5, 6, and 9 mRNA and protein expression and mRNA stability were measured. Adult cartilage was found to be t...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Asopa V, Vincent T, Saklatvala J Tags: Biomed Res Int Source Type: research

The Proosteogenic and Proangiogenic Effects of Small Extracellular Vesicles Derived from Bone Marrow Mesenchymal Stem Cells Are Attenuated in Steroid-Induced Osteonecrosis of the Femoral Head.
The objective of the present study was to compare the proosteogenic and proangiogenic effects of sEVs derived from BMMSCs from rats with steroid-induced ONFH (oBMMSCs-sEVs) and sEVs derived from BMMSCs from normal rats (nBMMSCs-sEVs). BMMSCs were isolated from steroid-induced ONFH rats and healthy rats. sEVs were isolated and characterized by Western blotting analysis of exosomal surface biomarkers and by transmission electron microscopy. The impacts of nBMMSCs-sEVs and oBMMSCs-sEVs on the proliferation and osteogenic differentiation of BMMSCs were determined via cell proliferation assay, alizarin red staining, and alkalin...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Li J, Ge Z, Ji W, Yuan N, Wang K Tags: Biomed Res Int Source Type: research

Pathological Features and Surgical Strategies of Cervical Deformity.
The objective in this article is to summarize studies on cervical scoliosis, identify clinical problems, and provide directions for researchers interested in delving deep into this specific topic. In this review, we found that the lack of standard classification system could lead to an absence of clinical guidance; in addition, the osseous landmarks and vascular distributions could be variable in CD patients, which might cause the risk of vascular or neurological complications; furthermore, multiple deformities were usually presented in CD patients, which might cause chain reaction after the correction of CD; this would pr...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Sun X, Sun S, Kong C, Wang W, Zhang T, Ding J, Li X, Lu S Tags: Biomed Res Int Source Type: research

Activation of Inward Rectifier K+ Channel 2.1 by PDGF-BB in Rat Vascular Smooth Muscle Cells through Protein Kinase A.
Abstract Platelet-derived growth factor-BB (PDGF-BB) can induce the proliferation, migration, and phenotypic modulation of vascular smooth muscle cells (VSMCs). We used patch clamp methods to study the effects of PDGF-BB on inward rectifier K+ channel 2.1 (Kir2.1) channels in rat thoracic aorta VSMCs (RASMCs). PDGF-BB (25 ng/mL) increased Kir2.x currents (-11.81 ± 2.47 pA/pF, P
Source: Biomed Res - May 30, 2020 Category: Research Authors: Tang C, Wang D, Luo E, Yan G, Liu B, Hou J, Qiao Y Tags: Biomed Res Int Source Type: research

Autophagy Suppresses Toll-Like Receptor 3-Mediated Inflammatory Reaction in Human Epidermal Keratinocytes.
Abstract Autophagy, one mechanism of programmed cell death, is fundamental to cellular homeostasis. Previous studies have identified autophagy as a novel mechanism by which cytokines control the immune response. However, its precise role in immune-related inflammatory skin diseases such as psoriasis remains unclear. Thus, this study explored the functional role of autophagy in psoriatic inflammation of epidermal keratinocytes. Strong light chain 3 immunoreactivity was observed in epidermal keratinocytes of both human psoriatic lesions and imiquimod-induced mice psoriatic model, and it was readily induced by polycy...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Li XM, Jung KE, Yim SH, Hong DK, Kim CD, Hong JY, Lee HJ, Lee SY, Kim JE, Park CW Tags: Biomed Res Int Source Type: research

Effects of Platelet-Rich Plasma on Proliferation, Viability, and Odontogenic Differentiation of Neural Crest Stem-Like Cells Derived from Human Dental Apical Papilla.
This study is aimed at evaluating the effects of platelet-rich plasma (PRP) on proliferation, viability, and odontogenic differentiation of neural crest stem-like cells (NCSCs) derived from human dental apical papilla. Materials and Methods: Cells from apical papillae were obtained and then induced to form neural spheres. The expression of NCSC markers p75NTR and HNK-1 in neural sphere cells was detected by immunofluorescence staining. Human PRP was prepared by a 2-step centrifugation method and activated by CaCl2 and thrombin. The concentrations of PDGF-BB and TGF-β1 in whole blood and PRP were measured by an EL...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Li J, Xiang L, Guan C, Yang X, Hu X, Zhang X, Zhang W Tags: Biomed Res Int Source Type: research

Evaluation of Zhenwu Decoction Effects on CYP450 Enzymes in Rats Using a Cocktail Method by UPLC-MS/MS.
Abstract This thesis is aimed at shedding light on the effects of the Zhenwu decoction (ZWD) on the activities and mRNA expressions of seven CYP450 isoenzymes. In the first step, we determined the main chemical compounds of ZWD by high-performance liquid chromatography (HPLC). Next, 48 male (SD) rats were randomly divided into the normal saline (NS) group and the ZWD low- (2.1875 g/kg), medium- (4.375 g/kg), and high- (8.75 g/kg) dose groups (12 per group). All rats were gavaged once daily for 28 consecutive days. A mixed solution of seven probe drugs was injected into 24 rats through the caud...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Hong LL, Wang Q, Zhao YT, Zhang S, Zhang KQ, Chen WD, Peng C, Liu L, Wang HS Tags: Biomed Res Int Source Type: research

Heme Oxygenase-1 Suppresses Wnt Signaling Pathway in Nonalcoholic Steatohepatitis-Related Liver Fibrosis.
Conclusion: Our study demonstrated that HO-1 inhibited the activation of canonical and noncanonical Wnt signaling pathways in NASH-related liver fibrosis. Thus, these results may suggest a new therapeutic strategy for NASH-related liver fibrosis. PMID: 32461992 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Du J, Ren W, Zhang Q, Fu N, Han F, Cui P, Li W, Kong L, Zhao S, Wang R, Zhang Y, Yang L, Kong L, Nan Y Tags: Biomed Res Int Source Type: research

Histopathology of Cutaneous Leishmaniasis Caused by Leishmania donovani in Sri Lanka.
Abstract Cutaneous leishmaniasis (CL) is a neglected tropical disease that is gaining importance in Sri Lanka and internationally. The clinical presentation, pathology, and method of parasite elimination in CL vary according to the species. Leishmania donovani is the causative organism for leishmaniasis in Sri Lanka. This collaborative cross-sectional study describes the clinicopathological features of cutaneous leishmaniasis among personnel of the tri-forces serving in the North and East of the country. The histology of fifty cases of CL confirmed by at least two methods (slit skin smear, lesion aspirate, tissue ...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Wijesinghe H, Gunathilaka N, Semege S, Pathirana N, Manamperi N, de Silva C, Fernando D Tags: Biomed Res Int Source Type: research

The Efficacy and Safety of PD-1/PD-L1 Inhibitors in Combination with Conventional Therapies for Advanced Solid Tumors: A Meta-Analysis.
Abstract Objectives: To evaluate the efficacy of immuno-oncology combinational therapy (IOCT) versus monotherapy with programmed cell death 1 (PD-1) or PD-ligand 1 (PD-L1) inhibitors or conventional therapies, i.e., non-IOCT, in patients with advanced solid tumors. Methods: We systematically searched the PubMed, Embase, and Cochrane Library databases from January 2015 to October 2018 for eligible studies. We included randomized trials of IOCT with available hazard ratios (HR) for death. The random effects model was used to calculate pooled HR for death; heterogeneity was assessed using I 2 statistics. The mai...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Nie RC, Zhao CB, Xia XW, Luo YS, Wu T, Zhou ZW, Yuan SQ, Wang Y, Li YF Tags: Biomed Res Int Source Type: research

Malnutrition-Related Factors Increased the Risk of Anastomotic Leak for Rectal Cancer Patients Undergoing Surgery.
This article will make a further study on this point. Methods: We collected perioperative clinical data from 382 patients with rectal cancer who underwent surgery from September 2015 to May 2017. After 1 month of follow-up, relevant risk factor data were collected and analyzed. Results: Data analysis showed that the incidence of AL was 14.65%. In single factor analysis, patients with high score of NRS-2002, high score of PG-SGA, diabetes, perioperative blood transfusion, postoperative diarrhea, later tumor stage, high score of ASA, low postoperative albumin, and rectal cancer patients with tumor close to the anus...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Xu H, Kong F Tags: Biomed Res Int Source Type: research

JMJD1A Represses the Development of Cardiomyocyte Hypertrophy by Regulating the Expression of Catalase.
Abstract The histone demethylase JMJD family is involved in various physiological and pathological functions. However, the roles of JMJD1A in the cardiovascular system remain unknown. Here, we studied the function of JMJD1A in cardiac hypertrophy. The mRNA and protein levels of JMJD1A were significantly downregulated in the hearts of human patients with hypertrophic cardiomyopathy and the hearts of C57BL/6 mice underwent cardiac hypertrophy induced by transverse aortic constriction (TAC) surgery or isoproterenol (ISO) infusion. In neonatal rat cardiomyocytes (NRCMs), siRNA-mediated JMJD1A knockdown facilitated ISO...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zang R, Tan Q, Zeng F, Wang D, Yu S, Wang Q Tags: Biomed Res Int Source Type: research

Lanthanum Chloride Causes Neurotoxicity in Rats by Upregulating miR-124 Expression and Targeting PIK3CA to Regulate the PI3K/Akt Signaling Pathway.
Conclusion: La causes neurotoxicity by upregulating miR-124 expression and targeting PIK3CA through the PI3K/Akt signaling pathway. PMID: 32461997 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zheng L, Zhang J, Yu S, Ding Z, Song H, Wang Y, Li Y Tags: Biomed Res Int Source Type: research

Gene-Gene Interaction Analysis for the Survival Phenotype Based on the Kaplan-Meier Median Estimate.
In this study, we propose a simple and computationally efficient method based on the multifactor dimensional reduction algorithm to identify gene-gene interactions associated with the survival phenotype. The proposed method, referred to as KM-MDR, uses the Kaplan-Meier median survival time as a classifier. The KM-MDR method classifies multilocus genotypes into a binary attribute for high- or low-risk groups using median survival time and replaces balanced accuracy with log-rank test statistics as a score to determine the best model. Through intensive simulation studies, we compared the power of KM-MDR with that of Surv-MDR...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Park M, Lee JW, Park T, Lee S Tags: Biomed Res Int Source Type: research

The Prevalence of Dental Caries in Primary Dentition in 3- to 5-Year-Old Preschool Children in Northern China.
Conclusions: The oral health status of children aged 3-5 years is not optimistic. Many parents have a low awareness of oral health. Strengthening the promotion of oral health knowledge is an effective way to change the situation. PMID: 32461999 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhang K, Li J, Lu Z Tags: Biomed Res Int Source Type: research

Identification and Validation of Prognostically Relevant Gene Signature in Melanoma.
Conclusions: We constructed a 6-gene signature (IQCE, RFX6, GPAA1, BAHCC1, CLEC2B, and AGAP2) as a novel prognostic marker for predicting the survival of melanoma patients. PMID: 32462000 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Gao Y, Li Y, Niu X, Wu Y, Guan X, Hong Y, Chen H, Song B Tags: Biomed Res Int Source Type: research

Quantitative Assessment of Motor Function for Patients with a Stroke by an End-Effector Upper Limb Rehabilitation Robot.
Abstract With the popularization of rehabilitation robots, it is necessary to develop quantitative motor function assessment methods for patients with a stroke. To make the assessment equipment easier to use in clinics and combine the assessment methods with the rehabilitation training process, this paper proposes an anthropomorphic rehabilitation robot based on the basic movement patterns of the upper limb, point-to-point reaching and circle drawing movement. This paper analyzes patients' movement characteristics in aspects of movement range, movement accuracy, and movement smoothness and the output force charact...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Liu Y, Song Q, Li C, Guan X, Ji L Tags: Biomed Res Int Source Type: research

The Optimal Regimen for the Treatment of Temporomandibular Joint Injury Using Low-Intensity Pulsed Ultrasound in Rats with Chronic Sleep Deprivation.
This study is aimed at observing the preventive and therapeutic effects of different modes of LIPUS and at identifying the optimal LIPUS treatment regimen for temporomandibular joint injury. In the present study, rat models of temporomandibular joint injury were established using a chronic sleep deprivation (CSD) method, and the effect of LIPUS as intensities of 30, 45, and 60 mW/cm2 was observed at 7, 14, and 21 days. After CSD, the condylar cartilage of the rats demonstrated variable degrees of surface roughening, collagen fiber disarrangement or even partial exfoliation, decreased proteoglycan synthesis and carti...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Liang C, Yang T, Wu G, Li J, Geng W Tags: Biomed Res Int Source Type: research

Immunologic and Clinical Failure of Antiretroviral Therapy in People Living with Human Immunodeficiency Virus within Two Years of Treatment.
This study is aimed at determining the prevalence of immunologic and clinical antiretroviral treatment failure. Methods: A cross-sectional study design using clinical and immunologic treatment failure definition was used to conduct the study. Sociodemographic characteristics and clinical features of patients were retrieved from patients' medical registry between the years 2009 and 2015. All patients who fulfilled the inclusion criteria in the study period were studied. Predictors of treatment failure were identified using Kaplan-Meier curves and multivariable Cox regression analysis. Data analysis was done using SPSS ...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Asgedom SW, Maru M, Berihun B, Gidey K, Niriayo YL, Atey TM Tags: Biomed Res Int Source Type: research

The Feasibility of All-Blastocyst-Culture and Single Blastocyst Transfer Strategy in Elderly Women: A Retrospective Analysis.
Abstract Objective: To investigate the feasibility and clinical outcome of the all-blastocyst-culture and single blastocyst transfer strategy in women aged ≥35 years. Methods: A retrospective analysis of patients aged ≥35 years undergoing IVF/ICSI was performed from January 2017 to April 2019 in the reproductive center of the Second Affiliated Hospital of Wenzhou Medical University. A total of 155 cases treated with ovarian hyperstimulation by prolonged protocol and implemented single (84 cases) or double (71 cases) blastocyst transfer were collected. Then, patients were further divided into
Source: Biomed Res - May 30, 2020 Category: Research Authors: Su W, Xu J, Arhin SK, Liu C, Zhao J, Lu X Tags: Biomed Res Int Source Type: research

A Centralized Outbreak of Varicella among Children Attending Preschool in Suzhou, China.
Abstract Background: Varicella vaccine is available for voluntary purchase with a single dose currently recommended for children aged ≥12 months. An epidemiological study was undertaken in order to determine the characteristics of the outbreak, assess vaccine effectiveness, and examine risk factors for vaccine failure. Methods: A varicella case was defined as a generalized papulovesicular rash (without other apparent causes) in a child without prior varicella attending the kindergarten during February 22 to April 7 of 2016. Varicella among vaccinated children (breakthrough varicella) was defined as varicel...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Zhang M, Gui GP, Guo F, Fan XF, Zha RS Tags: Biomed Res Int Source Type: research

Uncuffed Endotracheal Tube Experience in Pediatric Patients with Laparotomy and Laparoscopic Surgeries.
Conclusion: There was no significant difference between the groups in terms of tube changes and side effects. So that we can start with the largest possible uncuffed tube to decrease ETT leak, both laparotomy and laparoscopic operations in children can be achieved with safe mechanical ventilation and target tidal volume. PMID: 32462006 [PubMed - in process] (Source: Biomed Res)
Source: Biomed Res - May 30, 2020 Category: Research Authors: Şanal Baş S, Erdoğan Kayhan G, Onay M, Kılıç Y Tags: Biomed Res Int Source Type: research

A Revised Surgical Strategy for the Distal Tibiofibular Interosseous Osteochondroma.
Abstract Osteochondroma is one of the most common benign bone tumor; however, the surgical treatment still remains a challenge for those that occur at the distal tibiofibular interosseous location. Previously, the transfibular approach has been successfully described, but the potential damage of the syndesmosis would give rise to the instability of the ankle joint and thus may result in the unfavorable long-term outcome. Here, a revised strategy which can protect the syndesmotic complex is introduced. From 2010 to 2017, eleven patients with the distal tibiofibular interosseous osteochondroma who underwent the revi...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Yang H, Shou K, Wei S, Fang Z, Hu Q, Wan Q, Yang Y, Bao T Tags: Biomed Res Int Source Type: research

Extensively Drug-Resistant (XDR) Typhoid: Evolution, Prevention, and Its Management.
Abstract Typhoid fever is the result of a human host-restricted Salmonella enteric serotype typhi infection that causes enteric fever. Around 21 million people contract typhoid annually, with Pakistan's inhabitants at most risk amongst Asian countries where typhoid remains prevalent. Decades of indiscriminate antibiotic usage has driven the evolution of multidrug-resistant strains and more recently, extensively drug-resistant (XDR) strains of Salmonella enteric serotype typhi. Current reports of extensively drug-resistant typhoid fever outbreak in Pakistan are not only a major concern for Pakistan but also for hea...
Source: Biomed Res - May 30, 2020 Category: Research Authors: Akram J, Khan AS, Khan HA, Gilani SA, Akram SJ, Ahmad FJ, Mehboob R Tags: Biomed Res Int Source Type: research