Dissolution, bioactivity behavior, and cytotoxicity of 19.58Li < sub > 2 < /sub > O ·11.10ZrO < sub > 2 < /sub > ·69.32SiO < sub > 2 < /sub > glass-ceramic
J Biomed Mater Res B Appl Biomater. 2021 Jun 14. doi: 10.1002/jbm.b.34889. Online ahead of print.ABSTRACTGlass and bioactive glass-ceramic can be used in several applications. In bone growth where good bone/biomaterial adhesion was required, bioactive coatings for implants can improve bone formation. The glass and glass-ceramics of the LZS (Li2 O-ZrO2 -SiO2 ) system are very interesting because of their mechanical, electrical, and thermal properties. Very recently, their biological response in contact with human osteoblast has been evaluated. However, despite several initiatives, there are still no studies that systematica...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Nat ália Morelli Possolli Daiara Floriano da Silva Jaqueline Vieira Natasha Maurmann Patricia Pranke Ketner Bendo Dem étrio Elidio Angioletto Oscar Rubem Klegues Montedo Sabrina Arcaro Source Type: research

The Efficacy of Little Lovely Dentist, Dental Song, and Tell-Show-Do Techniques in Alleviating Dental Anxiety in Paediatric Patients: A Clinical Trial
CONCLUSION: The paediatric dental anxiety is a common finding in dental clinics. Behavior modification techniques like smartphone applications, "little lovely dentist," and "dental songs" can alleviate dental anxiety experienced by paediatric patients. The "tell-show-do" technique although most commonly used did not prove to be beneficial in the reduction of the anxiety levels.PMID:34124238 | PMC:PMC8166486 | DOI:10.1155/2021/1119710 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Hira Abbasi Muhammad Saqib Rizwan Jouhar Abhishek Lal Naseer Ahmed Muhammad Adeel Ahmed Mohammad Khursheed Alam Source Type: research

Importance of KIM-1 and MCP-1 in Determining the Leptospirosis-Associated AKI: A Sri Lankan Study
Biomed Res Int. 2021 Jun 2;2021:1752904. doi: 10.1155/2021/1752904. eCollection 2021.ABSTRACTBACKGROUND: Acute kidney injury (AKI) is one of most prevalent and serious complications of leptospirosis, a prevalent zoonotic disease in tropical countries. Prompt diagnosis of the leptospirosis-associated AKI is a challenge as there are no proper diagnostic tools that can identify patients in the early stage. Kidney injury molecule-1 (KIM-1) and monocyte chemoattractant protein-1 (MCP-1) are widely used novel AKI biomarkers that are studied in various disease conditions with AKI, but not in leptospirosis. Thus, this study is aim...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Thilini Nisansala Manjula Weerasekera Nilantha Ranasinghe Chamil Marasinghe Chandika Gamage Neluka Fernando Chinthika Gunasekara Source Type: research

Modelling the Transmission Dynamics of COVID-19 in Six High-Burden Countries
We presented an age-structured Susceptible-Latent-Mild-Critical-Removed (SLMCR) compartmental model of COVID-19 disease transmission with nonlinear incidence during the pandemic period. We provided the model calibration to estimate parameters with day-wise COVID-19 data, i.e., reported cases by worldometer from 15th February to 30th March 2020 in six high-burden countries, including Australia, Italy, Spain, the USA, the UK, and Canada. We estimate transmission rates for each country and found that the country with the highest transmission rate is Spain, which may increase the new cases and deaths than the other countries. ...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Azizur Rahman Md Abdul Kuddus Source Type: research

Titanium and Other Metal Hypersensitivity Diagnosed by MELISA ® Test: Follow-Up Study
This study is aimed at proving the clinical benefit of the MELISA® test in the minimization or complete elimination of health problems in patients with confirmed hypersensitivity to metals used for tissue replacements. A group of 305 patients aged 20-75 years with previously proven metal hypersensitivity (initial MELISA® test), mainly to titanium and then to another fifteen metals, was chosen from the database at the Institute of Dental Medicine. From these patients, a final group of 42 patients agreed to participate in the study, 35 of which were female and 7 were male. The patients completed a special questionnai...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Radka Vrbova Stepan Podzimek Lucie Himmlova Adela Roubickova Marketa Janovska Tatjana Janatova Martin Bartos Alex Vinsu Source Type: research

Identifying Mitochondrial-Related Genes NDUFA10 and NDUFV2 as Prognostic Markers for Prostate Cancer through Biclustering
Biomed Res Int. 2021 May 22;2021:5512624. doi: 10.1155/2021/5512624. eCollection 2021.ABSTRACTProstate cancer is currently associated with higher morbidity and mortality in men in the United States and Western Europe, so it is important to identify genes that regulate prostate cancer. The high-dimension gene expression profile impedes the discovery of biclusters which are of great significance to the identification of the basic cellular processes controlled by multiple genes and the identification of large-scale unknown effects hidden in the data. We applied the biclustering method MCbiclust to explore large biclusters in ...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Haokun Zhang Yuanhua Shao Weijun Chen Xin Chen Source Type: research

Different Outcomes of Anti-VEGF Treatment for Neovascular AMD according to Neovascular Sutypes and Baseline Features: 2-Year Real-Life Clinical Outcomes
Biomed Res Int. 2021 May 24;2021:5516981. doi: 10.1155/2021/5516981. eCollection 2021.ABSTRACTPURPOSE: To evaluate the effects of anti-VEGF treatment of neovascular age-related macular degeneration (nAMD) in a real-life clinical setting.METHODS: Study design is a retrospective case series. Naïve nAMD patients treated with intravitreal injection of aflibercept or ranibizumab were analyzed over a 24-month follow-up. Each patient received the loading dose, followed by a PRN regimen. Patients were further subdivided into subgroups according to macular neovascularization type, best corrected visual acuity (BCVA) at baselin...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Alessandro Arrigo Andrea Saladino Emanuela Aragona Stefano Mercuri Ugo Introini Francesco Bandello Maurizio Battaglia Parodi Source Type: research

The Influence of Serum Uric Acid Level on Alzheimer's Disease: A Narrative Review
Biomed Res Int. 2021 Jun 2;2021:5525710. doi: 10.1155/2021/5525710. eCollection 2021.ABSTRACTAs a powerful antioxidant in the human body, uric acid (UA) has been the subject of increasing research that focused on its influence on Alzheimer's disease (AD) in recent years. The latest literature was gathered to describe the influence of serum uric acid (SUA) level on the onset and progression of AD and to analyze the possibility that SUA is a biomarker of Alzheimer's disease. A large number of existing studies suggested that the SUA level was lower or tended to decrease in patients with AD, and increased SUA level may have a ...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Mengyuan Qiao Chongli Chen Yuqing Liang Yuxi Luo Wenbin Wu Source Type: research

The Application of Digital Design Combined with 3D Printing Technology in Skin Flap Transplantation for Fingertip Defects during the COVID-19 Epidemic
CONCLUSIONS: During the COVID-19 epidemic, fingertip defects were treated with preoperative digital design of fingertip defect flaps combined with 3D printing. Precision design saves surgery time and improves the success rate of surgery and the survival rates of skin flaps. In addition, 3D model simulations improve preoperative communication efficiency, and the personalized design improves patient satisfaction.PMID:34124245 | PMC:PMC8189776 | DOI:10.1155/2021/5554500 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Hui Lu Hanshu Peng Ze Peng Dingxi Liu Qimei Wu Rong Liu Source Type: research

Effects of TheraBand and Theratube Eccentric Exercises on Quadriceps Muscle Strength and Muscle Mass in Young Adults
CONCLUSION: Both the TheraBand and theratube are equally effective in the strengthening of the quadriceps muscle in young adults. Therefore, either the TheraBand or theratube may be used according to the availability and feasibility of the subjects for training intervention.PMID:34124246 | PMC:PMC8172275 | DOI:10.1155/2021/5560144 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Shahnawaz Anwer Shayista I Jeelani Sohrab Ahmad Khan Nishat Quddus Sumit Kalra Ahmad H Alghadir Source Type: research

Multichannel Retinal Blood Vessel Segmentation Based on the Combination of Matched Filter and U-Net Network
In this study, we use a multiscale matched filter with vessel enhancement capability and a U-Net model with a coding and decoding network structure. Three channels are used to extract vessel features separately, and finally, the segmentation results of the three channels are merged. The algorithm proposed in this paper has been verified and evaluated on the DRIVE, STARE, and CHASE_DB1 datasets. The experimental results show that the proposed algorithm can segment small blood vessels better than most other methods. We conclude that our algorithm has reached 0.8745, 0.8903, and 0.8916 on the three datasets in the sensitivity...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Yuliang Ma Zhenbin Zhu Zhekang Dong Tao Shen Mingxu Sun Wanzeng Kong Source Type: research

Score for Predicting Active Cancer in Patients with Ischemic Stroke: A Retrospective Study
Biomed Res Int. 2021 May 25;2021:5585206. doi: 10.1155/2021/5585206. eCollection 2021.ABSTRACTBACKGROUND: We aimed to examine the differences of clinical characteristics between patients with ischemic stroke with active cancer and those without cancer to develop a clinical score for predicting the presence of occult cancer in patients with ischemic stroke.METHODS: This retrospective study enrolled consecutive adult patients with acute ischemic stroke who were admitted to our department between December 2017 and January 2019. The demographic, clinical, laboratory, and neuroimaging characteristics were compared between patie...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Jiwei Jiang Xiuli Shang Jinming Zhao Meihui Cao Jirui Wang Runzhi Li Yanli Wang Jun Xu Source Type: research

Evaluation of Fibroblast Viability Seeded on Acellular Human Amniotic Membrane
CONCLUSION: Our findings showed the viability of fibroblasts seeded on the acellular HAM using MTT assay, DAPI, and PI staining; however, this study had some limitations. It would be an interesting subject for future research to compare the viability and proliferation rate of fibroblasts seeded on both cellular and acellular HAM.PMID:34124249 | PMC:PMC8169243 | DOI:10.1155/2021/5597758 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Hamideh Moravvej Hamed Memariani Mojtaba Memariani Maryam Kabir-Salmani Alireza Shoae-Hassani Fahimeh Abdollahimajd Source Type: research

Analysis of Facial Skeletal Morphology: Nasal Bone, Maxilla, and Mandible
In conclusion, nasal and maxillary dimensions exhibited no significant difference among the 3 skeletal patterns. Mandibular body and entire mandibular lengths were significantly positively correlations with Class III skeletal patterns.PMID:34124250 | PMC:PMC8169245 | DOI:10.1155/2021/5599949 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Han-Sheng Chen Szu-Yu Hsiao Kun-Tsung Lee Source Type: research

Identification and Verification of a 17 Immune-Related Gene Pair Prognostic Signature for Colon Cancer
CONCLUSION: We have successfully established a signature model composed of 17 IRGPs, which provides a novel idea to predict the prognosis of CC patients.PMID:34124251 | PMC:PMC8166469 | DOI:10.1155/2021/6057948 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Qianshi Zhang Zhen Feng Yongnian Zhang Shasha Shi Yu Zhang Shuangyi Ren Source Type: research

Topography and Anatomical Variations of the Axillary Artery
Biomed Res Int. 2021 May 24;2021:6393780. doi: 10.1155/2021/6393780. eCollection 2021.ABSTRACTKnowledge of anatomical variations of the limb's main arteries is significant for the clinicians. Thus, this study is aimed at examining the branching pattern and anatomical variations of the axillary artery. We conducted research on 59 upper limbs of adult human donated cadavers. All axillary artery branches' origins were assessed, and the correlations between points of origins and variations of specific branches were evaluated. The average length of the axillary artery was found to be 11.22 cm, and this length was defined as ref...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Kiwook Yang Hyunsu Lee In-Jang Choi Woonhyeok Jeong Hong-Tae Kim Qun Wei Jae-Ho Lee Source Type: research

Overexpression of RIPK4 Predicts Poor Prognosis and Promotes Metastasis in Ovarian Cancer
This study was conducted to evaluate the prognostic value of receptor-interacting protein kinase 4 (RIPK4) in ovarian cancer (OC) and its role in tumorigenesis. RNA expression and the corresponding clinical data were obtained from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. The relationship between clinical-pathological characteristics and RIPK4 expression was analyzed using the Wilcoxon signed-rank test and logistic regression. The Cox regression and the Kaplan-Meier method were used to evaluate the relationship between clinicopathological features and overall survival (OS). Gene set en...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Shaoqiu Liu Lewei He Chenchen Sheng Rongjia Su Xiaomei Wu Yunyan Sun Xiaowei Xi Source Type: research

F1012-2 Induced ROS-Mediated DNA Damage Response through Activation of MAPK Pathway in Triple-Negative Breast Cancer
In conclusion, the activity of F1012-2-induced DNA damage in TNBC was found in vivo and in vitro, which might trigger the MAPK pathway through ROS accumulation. These results indicate that F1012-2 may be an effective anti-TNBC therapeutic agent.PMID:34124254 | PMC:PMC8189789 | DOI:10.1155/2021/6650045 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Lingjie Dai Shasha Tian Jinyao Zhang Mengyuan Lu Jingchao Zhu Huajun Zhao Source Type: research

Tumor Microenvironment Subtypes and Immune-Related Signatures for the Prognosis of Breast Cancer
CONCLUSIONS: We developed and validated a prognostic model based on immune-related signatures for breast cancer. This promising model is justified for validation and optimized in future clinical practice.PMID:34124255 | PMC:PMC8189770 | DOI:10.1155/2021/6650107 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Yiqun Han Jiayu Wang Binghe Xu Source Type: research

Seroprevalence and Associated Factors of Human Immunodeficiency Virus, < em > Treponema pallidum < /em > , Hepatitis B Virus, and Hepatitis C Virus among Female Sex Workers in Dessie City, Northeast Ethiopia
Biomed Res Int. 2021 May 25;2021:6650333. doi: 10.1155/2021/6650333. eCollection 2021.ABSTRACTINTRODUCTION: Sexually transmitted infections (STIs) are prevalent in Ethiopia and elsewhere among different population groups particularly among female sex workers (FSWs). Because of their work and their behavior, FSWs are at high risk to acquire STIs. The aim of the study was to assess the seroprevalence and associated factors of HIV, HBV, HCV, and T. pallidum among FSWs in Dessie City, Northeast Ethiopia.METHODS: This cross-sectional study was conducted in Dessie City, Amhara Region, Northeastern Ethiopia, from November 2017 to...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Yeshi Metaferia Abdurahaman Ali Solomon Eshetu Daniel Gebretsadik Source Type: research

Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction
Biomed Res Int. 2021 Jun 8;2021:6653950. doi: 10.1155/2021/6653950. eCollection 2021.ABSTRACTThe study is aimed at establishing the optimal parameters for RNA purification of pooled specimens, in SARS-CoV-2 assay. This research work evaluates the difference of extracted RNA purity of pooled samples with and without treatment with isopropyl alcohol and its effect on real-time RT-PCR. As per the protocol of the Indian Council of Medical Research (ICMR), 5 sample pools were analysed using qRT-PCR. A total of 100 pooled samples were selected for the study by mixing 50 μL of one COVID-19 positive nasopharyngeal/oropharyngeal...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Mayank Gangwar Alka Shukla Virendra Kumar Patel Pradyot Prakash Gopal Nath Source Type: research

Pain Management through Neurocognitive Therapeutic Exercises in Hypermobile Ehlers-Danlos Syndrome Patients with Chronic Low Back Pain
CONCLUSION: The clinical results observed in our study seem to confirm the role of a specific neurocognitive rehabilitation program in the chronic pain management in the Ehlers-Danlos syndrome; the rehabilitation treatment should be tailored on patient problems and focused not only in the recovery of movement but also on pain perception.PMID:34124258 | PMC:PMC8189767 | DOI:10.1155/2021/6664864 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Claudia Celletti Teresa Paolucci Loredana Maggi Giordana Volpi Mariangela Billi Roberta Mollica Filippo Camerota Source Type: research

Active Learning and the Potential of Neural Networks Accelerate Molecular Screening for the Design of a New Molecule Effective against SARS-CoV-2
Biomed Res Int. 2021 May 25;2021:6696012. doi: 10.1155/2021/6696012. eCollection 2021.ABSTRACTA global pandemic has emerged following the appearance of the new severe acute respiratory virus whose official name is the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), strongly affecting the health sector as well as the world economy. Indeed, following the emergence of this new virus, despite the existence of a few approved and known effective vaccines at the time of writing this original study, a sense of urgency has emerged worldwide to discover new technical tools and new drugs as soon as possible. In this con...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Rabhi Yassine Mrabet Makrem Fnaiech Farhat Source Type: research

Analysis of the Efficacy and Mechanism of Action of Xuebijing Injection on ARDS Using Meta-Analysis and Network Pharmacology
CONCLUSION: Our results recommended Xuebijing treatment for patients with ARDS. Xuebijing has therapeutic effects on ARDS patients partly by regulating the immune cell/cytokine pathways and thus inhibiting the cytokine storm. TNF-α is the cytokine both directly and indirectly inhibited by Xuebijing, and IL-6 is the cytokine mainly indirectly inhibited by Xuebijing.PMID:34124260 | PMC:PMC8166476 | DOI:10.1155/2021/8824059 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Yun Zhang Jie Wang Yong-Mei Liu Hui Yang Guang-Jun Wu Xuan-Hui He Source Type: research

Efficacy Evaluation of 3D Navigational Template for Salter Osteotomy of DDH in Children
Biomed Res Int. 2021 May 22;2021:8832617. doi: 10.1155/2021/8832617. eCollection 2021.ABSTRACTBACKGROUND: The aim of this study is to retrospectively evaluate the efficacy of 3D navigational template for Salter osteotomy of DDH in children.METHODS: Thirty-two consecutive patients with DDH who underwent Salter osteotomy were evaluated between July 2014 and August 2017, and they were divided into the conventional group (n = 16) and navigation template group (n = 16) according to different surgical methods. The corrective acetabular degrees, radiation exposure, and operation time were compared between the two groups.RESULTS: ...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Jie Yu Qiang Shi Source Type: research

Altered Genes and Biological Functions in Response to Severe Burns
Biomed Res Int. 2021 May 24;2021:8836243. doi: 10.1155/2021/8836243. eCollection 2021.ABSTRACTSevere burns are acute wounds caused by local heat exposure, resulting in life-threatening systemic effects and poor survival. However, the specific molecular mechanisms remain unclear. First, we downloaded gene expression data related to severe burns from the GEO database (GSE19743, GSE37069, and GSE77791). Then, a gene expression analysis was performed to identify differentially expressed genes (DEGs) and construct protein-protein interaction (PPI) network. The molecular mechanism was identified by enrichment analysis and Gene S...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Xinheng Liu Yongxian Rong Donglin Huang Zhijie Liang Xiaolin Yi Fangxiao Wu Dandan Zhu Steven Mo Wenhai Nong Hongmian Li Source Type: research

Voltage-Gated Sodium Channel ( < em > Vgsc < /em > ) Mutation-Based Pyrethroid Resistance in < em > Aedes aegypti < /em > Populations of Three Endemic Dengue Risk Areas of Sri Lanka
CONCLUSIONS: The findings clearly indicate that long-term insecticide applications and multiple use of pyrethroids have led to the acquisition of kdr mutations, leading to the development of insecticide resistance among local Ae. aegypti populations, especially in the Colombo and Gampaha districts. Therefore, evaluation of the prevalence levels of these kdr mutations highlights the necessity for shifting towards novel vector control strategies.PMID:34124263 | PMC:PMC8166465 | DOI:10.1155/2021/8874092 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Tharaka Ranathunge Lahiru Udayanga Sumudu Sarasija Samudra Karunathilaka Shavindhya Nawarathne Haruthra Rathnarajah Fathima Fazla Dulficar Fathima Nafla Shafi Ranil S Dassanayake Y I Nilmini Silva Gunawardene Source Type: research

High FAM189B Expression and Its Prognostic Value in Patients with Gastric Cancer
In conclusion, the overexpression of the FAM189B protein and mRNA might enhance the incidence of GC.PMID:34124264 | PMC:PMC8172284 | DOI:10.1155/2021/8875971 (Source: Biomed Res)
Source: Biomed Res - June 14, 2021 Category: Research Authors: Chang-Liang Wu Quan-Xiao Tan Dan Liu Jun-E Jiang Ting Lu Zu-Mei Huang Ying-Jie Su Source Type: research

PD-1 Coexpression Gene Analysis and the Regulatory Network in Endometrial Cancer Based on Bioinformatics Analysis
Biomed Res Int. 2021 May 25;2021:9923434. doi: 10.1155/2021/9923434. eCollection 2021.ABSTRACTGynecological malignancies are tumors of the female reproductive system, mainly cervical cancer, endometrial cancer, and ovarian cancer. Endometrial cancer (EC) is the most common gynecological malignant tumor in developed countries. The aim of this study was to construct a network of programmed cell death protein 1 (PD-1) coexpressed genes through bioinformatics analysis and screen the potential biomarkers of PD-1 in endometrial cancer. In addition, genes and pathways involved in PD-1 and modulating tumor immune status were ident...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Lina Wang Zhen Liu Wenwen Zhang Aihua Zhang Pengpeng Qu Source Type: research

Trends of Antibacterial Resistance at the National Reference Laboratory in Cameroon: Comparison of the Situation between 2010 and 2017
Biomed Res Int. 2021 May 22;2021:9957112. doi: 10.1155/2021/9957112. eCollection 2021.ABSTRACTINTRODUCTION: Antimicrobial resistance represents a growing public health threat. One of the World Health Organization's strategic objectives is "strengthening knowledge through surveillance and research." Sub-Saharan African countries are still far from achieving this objective. We aimed to estimate and compare the prevalence of antibacterial resistance in 2010 and 2017 in Cameroon.METHODS: We conducted a retrospective study on all clinical specimens cultured in Centre Pasteur du Cameroun (CPC) in 2010 and 2017. Data we...
Source: Biomed Res - June 14, 2021 Category: Research Authors: M Massongo L Ngando E W Pefura Yone Ariane NZouankeu W Mbanzouen M C Fonkoua A Ngandjio J Tchatchueng D Barger M C Tejiokem Source Type: research

Antibacterial Potency of Medicinal Plants including < em > Artemisia annua < /em > and < em > Oxalis corniculata < /em > against Multi-Drug Resistance < em > E. coil < /em >
Biomed Res Int. 2021 Jun 1;2021:9981915. doi: 10.1155/2021/9981915. eCollection 2021.ABSTRACTAntibacterial activity of ethanolic and aqueous extracts of two medicinal plants including Oxalis corniculata (EtOc, AqOc) and Artemisia annua (EtAa, AqAa) as well as A. annua essential oil (EoAa) was investigated on multi-drug resistance (MDR) E. coli. Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) as well as the inhibition zone. The phytconstituents of these products were analyzed using Reverse-phase High- performance liquid...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Hassan Golbarg Mohammad Javad Mehdipour Moghaddam Source Type: research

The Study on the Pathogenesis of Pediatric Lymphoma Based on the Combination of Pseudotargeted and Targeted Metabolomics
Biomed Res Int. 2021 May 25;2021:9984357. doi: 10.1155/2021/9984357. eCollection 2021.ABSTRACTPediatric lymphoma is a kind of malignant tumor with high mortality. The complexity of pediatric lymphoma shows a great challenge for effective diagnosis and treatment. In order to meet the challenge, the combination of pseudotargeted and targeted metabolomics was used to analyze the serum metabolites in pediatric lymphoma patients and healthy controls for discovering the metabolites related to pediatric lymphoma. The serum samples were obtained from the treatment group (n = 43), the control group (n = 26), and the patients group ...
Source: Biomed Res - June 14, 2021 Category: Research Authors: Hongqi Sun Nan Chen Xuchen Wang Na Li Shanshan Wang Zhengyan Zhang Ying Zhou Junmei Yang Source Type: research

Ginsenoside Rk3 alleviates gut microbiota dysbiosis and colonic inflammation in antibiotic-treated mice
In conclusion, Rk3 improves intestinal inflammation and induces potentially beneficial changes in the gut microbiota, and these findings help elucidate host-microbe interactions.PMID:34119248 | DOI:10.1016/j.foodres.2021.110465 (Source: Biomed Res)
Source: Biomed Res - June 13, 2021 Category: Research Authors: Xue Bai Rongzhan Fu Zhiguang Duan Pan Wang Chenhui Zhu Daidi Fan Source Type: research

Platelet-like particles reduce coagulopathy-related and neuroinflammatory pathologies post-experimental traumatic brain injury
The objective of this study was to diminish coagulopathy in a mouse TBI model (controlled cortical impact) via PLP treatment, and subsequently decrease blood-brain barrier (BBB) permeability and neuroinflammation. Following an acute intravenous injection of PLPs post-TBI, we analyzed BBB permeability, ex vivo coagulation parameters, and neuroinflammation at 24 hr and 7 days post-TBI. Both PLP-treatment and control particle-treatment had significantly decreased BBB permeability and improved clot structure 24 hr post-injury. Additionally, no significant change in tissue sparing was observed between 24 hr and 7 days for PLP-t...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Jordan Todd Vimala N Bharadwaj Kimberly Nellenbach Seema Nandi Emily Mihalko Connor Copeland Ashley C Brown Sarah E Stabenfeldt Source Type: research

Chitosan and alginate salt as biomaterials are potential natural adjuvants for killed cholera vaccine
J Biomed Mater Res A. 2021 Jun 11. doi: 10.1002/jbm.a.37240. Online ahead of print.ABSTRACTChitosan and alginate salts are natural biopolymers that have gained recent attention in the biomedical sectors. Their properties allow them to become potential candidates as safe, cheap, and effective vaccine adjuvants. The present study aimed to enhance the immunogenic response of a current injectable killed cholera vaccine (KCV) using chitosan and alginate salt as natural adjuvants against alum. We tested KCV adjuvanted with alum, chitosan, and sodium alginate in mice. Mice were immunized intraperitoneally with KCV adjuvanted with...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Nourhan H AbdelAllah Yasser Gaber Sameh AbdelGhani Mohamed E Rashed Ahmed F Azmy Source Type: research

Platelet-like particles reduce coagulopathy-related and neuroinflammatory pathologies post-experimental traumatic brain injury
The objective of this study was to diminish coagulopathy in a mouse TBI model (controlled cortical impact) via PLP treatment, and subsequently decrease blood-brain barrier (BBB) permeability and neuroinflammation. Following an acute intravenous injection of PLPs post-TBI, we analyzed BBB permeability, ex vivo coagulation parameters, and neuroinflammation at 24 hr and 7 days post-TBI. Both PLP-treatment and control particle-treatment had significantly decreased BBB permeability and improved clot structure 24 hr post-injury. Additionally, no significant change in tissue sparing was observed between 24 hr and 7 days for PLP-t...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Jordan Todd Vimala N Bharadwaj Kimberly Nellenbach Seema Nandi Emily Mihalko Connor Copeland Ashley C Brown Sarah E Stabenfeldt Source Type: research

Chitosan and alginate salt as biomaterials are potential natural adjuvants for killed cholera vaccine
J Biomed Mater Res A. 2021 Jun 11. doi: 10.1002/jbm.a.37240. Online ahead of print.ABSTRACTChitosan and alginate salts are natural biopolymers that have gained recent attention in the biomedical sectors. Their properties allow them to become potential candidates as safe, cheap, and effective vaccine adjuvants. The present study aimed to enhance the immunogenic response of a current injectable killed cholera vaccine (KCV) using chitosan and alginate salt as natural adjuvants against alum. We tested KCV adjuvanted with alum, chitosan, and sodium alginate in mice. Mice were immunized intraperitoneally with KCV adjuvanted with...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Nourhan H AbdelAllah Yasser Gaber Sameh AbdelGhani Mohamed E Rashed Ahmed F Azmy Source Type: research

Platelet-like particles reduce coagulopathy-related and neuroinflammatory pathologies post-experimental traumatic brain injury
The objective of this study was to diminish coagulopathy in a mouse TBI model (controlled cortical impact) via PLP treatment, and subsequently decrease blood-brain barrier (BBB) permeability and neuroinflammation. Following an acute intravenous injection of PLPs post-TBI, we analyzed BBB permeability, ex vivo coagulation parameters, and neuroinflammation at 24 hr and 7 days post-TBI. Both PLP-treatment and control particle-treatment had significantly decreased BBB permeability and improved clot structure 24 hr post-injury. Additionally, no significant change in tissue sparing was observed between 24 hr and 7 days for PLP-t...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Jordan Todd Vimala N Bharadwaj Kimberly Nellenbach Seema Nandi Emily Mihalko Connor Copeland Ashley C Brown Sarah E Stabenfeldt Source Type: research

Chitosan and alginate salt as biomaterials are potential natural adjuvants for killed cholera vaccine
J Biomed Mater Res A. 2021 Jun 11. doi: 10.1002/jbm.a.37240. Online ahead of print.ABSTRACTChitosan and alginate salts are natural biopolymers that have gained recent attention in the biomedical sectors. Their properties allow them to become potential candidates as safe, cheap, and effective vaccine adjuvants. The present study aimed to enhance the immunogenic response of a current injectable killed cholera vaccine (KCV) using chitosan and alginate salt as natural adjuvants against alum. We tested KCV adjuvanted with alum, chitosan, and sodium alginate in mice. Mice were immunized intraperitoneally with KCV adjuvanted with...
Source: Biomed Res - June 12, 2021 Category: Research Authors: Nourhan H AbdelAllah Yasser Gaber Sameh AbdelGhani Mohamed E Rashed Ahmed F Azmy Source Type: research

Family-Centered Social Cognitive Factors Preventing Primary Tooth Caries in Children Based on Intervention Mapping Approach
Biomed Res Int. 2021 May 18;2021:6626090. doi: 10.1155/2021/6626090. eCollection 2021.ABSTRACTBACKGROUND: Given the increasing prevalence of primary tooth caries in Iran and the importance of providing evidence- and theory-based family-centered prevention programs, the present study is aimed at determining the family-centered social cognitive factors preventing deciduous tooth caries among children using the intervention mapping protocol.METHODS: This cross-sectional study was performed on 240 Iranian mothers in Ilam who were randomly selected to participate in the study. The data were collected using a self-designed quest...
Source: Biomed Res - June 11, 2021 Category: Research Authors: Bahareh Kabiri Ali Reza Hidarnia Mehdi Mirzaei Alavijeh Mohammad Esmaeel Motlagh Ali Montazeri Source Type: research

Imaging Assessment of the Postoperative Spine: An Updated Pictorial Review of Selected Complications
This article presents a pictorial narrative review of the most common findings observed in noninstrumented and instrumented postoperative spines. Complications in the noninstrumented spine were grouped in early (hematomas, pseudomeningocele, and postoperative spine infection) and late findings (arachnoiditis, radiculitis, recurrent disc herniation, spinal stenosis, and textiloma). Complications in the instrumented spine were also sorted in early (hardware fractures) and late findings (adjacent segment disease, hardware loosening, and implant migration). This review also includes a short description of the most used diagnos...
Source: Biomed Res - June 11, 2021 Category: Research Authors: Roberto Corona-Cedillo Melanie-Tessa Saavedra-Navarrete Juan-Jose Espinoza-Garcia Alexela-Nerey Mendoza-Aguilar Sergey K Ternovoy Ernesto Roldan-Valadez Source Type: research

Combining stem cells in myocardial infarction: The road to superior repair?
Med Res Rev. 2021 Jun 11. doi: 10.1002/med.21839. Online ahead of print.ABSTRACTMyocardial infarction irreversibly destroys millions of cardiomyocytes in the ventricle, making it the leading cause of heart failure worldwide. Over the past two decades, many progenitor and stem cell types were proposed as the ideal candidate to regenerate the heart after injury. The potential of stem cell therapy has been investigated thoroughly in animal and human studies, aiming at cardiac repair by true tissue replacement, by immune modulation, or by the secretion of paracrine factors that stimulate endogenous repair processes. Despite so...
Source: Biomed Res - June 11, 2021 Category: Research Authors: Hanne Beli ën Lize Evens Marc Hendrikx Virginie Bito Annelies Bronckaers Source Type: research

The evaluation of a multiphasic 3D-bioplotted scaffold seeded with adipose derived stem cells to repair osteochondral defects in a porcine model
The objective of this study was to evaluate this scaffold in a large animal model. Osteochondral defects were generated in the trochlear groove of Yucatan minipigs and repaired with scaffolds that either contained or lacked an electrospun tidemark and were either unseeded or seeded with ASC. Implants were monitored via computed tomography (CT) over the course of 4 months of in vivo implantation and compared to both open lesions and autologous explants. ICRS II evaluation indicated that defects with ASC-seeded scaffolds had healing that most closely resembled the aulogous explant. Scaffold-facilitated subchondral bone repai...
Source: Biomed Res - June 11, 2021 Category: Research Authors: Rachel C Nordberg Pedro Huebner Karl G Schuchard Liliana F Mellor Rohan A Shirwaiker Elizabeth G Loboa Jeffery T Spang Source Type: research

Drug resistance mutations in a population prior to antiretroviral therapy initiation in Northern South Africa
AIDS Res Hum Retroviruses. 2021 Jun 9. doi: 10.1089/AID.2021.0026. Online ahead of print.ABSTRACTSouth Africa introduced the "diagnose and treat" HIV treatment programme in September 2016. The programme enables diagnosed HIV positive patients to start treatment. However, the presence of drug resistant (DR) viruses in the drug naïve population complicates the choice of ART. We used next generation sequencing (NGS) to determine the prevalence and diversity of HIV DR mutations in patients entering HIV treatment programmes in northern South Africa. RNA was isolated from the plasma of drug naive HIV-1 infected pa...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Bixa Okech Ogola Nontokozo D Matume Lufuno Grace Mavhandu Denis Manga Tebit Pascal Obong Bessong Source Type: research

Preparation of a water soluble aminated β-1,3-D-glucan for gene carrier: The in vitro study of the anti-inflammatory activity and transfection efficiency
J Biomed Mater Res A. 2021 Jun 10. doi: 10.1002/jbm.a.37244. Online ahead of print.ABSTRACTβ-1,3-D-glucan has been reported to have a series of bioactivities including antitumor, antimicrobial, anti-inflammatory and antioxidative effects; however, its insolubility in neutral aqueous solution significantly restricts the potential application in biological and medicine fields. Herein, a water-soluble aminated β-1,3-D-glucan (AG) was synthesized and the anti-inflammatory effect, cytotoxicity and plasmid DNA (pDNA) binding capacity of AG, serum stability, the particle sizes and zeta potentials of AG/pDNA nanocomposit...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Jing Wei Jin Min Zhi Rong Ming Qiu Zhang Wing-Leung Wong Source Type: research

Mg(OH) < sub > 2 < /sub > nanoparticles enhance the antibacterial activities of macrophages by activating the reactive oxygen species
In this study, Escherichia coli was selected as the pathogenic bacteria and it was found that Mg(OH)2 NPs significantly inhibited the growth of E. coli by promoting nucleic acid leakage, inhibiting protein synthesis, and suppressing the metabolic activity. The minimum inhibitory concentration for these bacteria was determined to be 4.4 μg/ml. In vitro flow cytometry and immunofluorescence tests indicated that Mg(OH)2 NPs induced the macrophages to generate reactive oxygen species to kill the bacteria. To understand the mechanisms involved in this process, western blotting was performed and it was found that Mg(OH)2 NPs ...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Yong Zhu Yifu Tang Zhe Ruan Yilong Dai Zhaohui Li Zhangyuan Lin Shushan Zhao Liang Cheng Buhua Sun Ming Zeng Jianxi Zhu Ruibo Zhao Bangbao Lu Haitao Long Source Type: research

Drug resistance mutations in a population prior to antiretroviral therapy initiation in Northern South Africa
AIDS Res Hum Retroviruses. 2021 Jun 9. doi: 10.1089/AID.2021.0026. Online ahead of print.ABSTRACTSouth Africa introduced the "diagnose and treat" HIV treatment programme in September 2016. The programme enables diagnosed HIV positive patients to start treatment. However, the presence of drug resistant (DR) viruses in the drug naïve population complicates the choice of ART. We used next generation sequencing (NGS) to determine the prevalence and diversity of HIV DR mutations in patients entering HIV treatment programmes in northern South Africa. RNA was isolated from the plasma of drug naive HIV-1 infected pa...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Bixa Okech Ogola Nontokozo D Matume Lufuno Grace Mavhandu Denis Manga Tebit Pascal Obong Bessong Source Type: research

Preparation of a water soluble aminated β-1,3-D-glucan for gene carrier: The in vitro study of the anti-inflammatory activity and transfection efficiency
J Biomed Mater Res A. 2021 Jun 10. doi: 10.1002/jbm.a.37244. Online ahead of print.ABSTRACTβ-1,3-D-glucan has been reported to have a series of bioactivities including antitumor, antimicrobial, anti-inflammatory and antioxidative effects; however, its insolubility in neutral aqueous solution significantly restricts the potential application in biological and medicine fields. Herein, a water-soluble aminated β-1,3-D-glucan (AG) was synthesized and the anti-inflammatory effect, cytotoxicity and plasmid DNA (pDNA) binding capacity of AG, serum stability, the particle sizes and zeta potentials of AG/pDNA nanocomposit...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Jing Wei Jin Min Zhi Rong Ming Qiu Zhang Wing-Leung Wong Source Type: research

Mg(OH) < sub > 2 < /sub > nanoparticles enhance the antibacterial activities of macrophages by activating the reactive oxygen species
In this study, Escherichia coli was selected as the pathogenic bacteria and it was found that Mg(OH)2 NPs significantly inhibited the growth of E. coli by promoting nucleic acid leakage, inhibiting protein synthesis, and suppressing the metabolic activity. The minimum inhibitory concentration for these bacteria was determined to be 4.4 μg/ml. In vitro flow cytometry and immunofluorescence tests indicated that Mg(OH)2 NPs induced the macrophages to generate reactive oxygen species to kill the bacteria. To understand the mechanisms involved in this process, western blotting was performed and it was found that Mg(OH)2 NPs ...
Source: Biomed Res - June 10, 2021 Category: Research Authors: Yong Zhu Yifu Tang Zhe Ruan Yilong Dai Zhaohui Li Zhangyuan Lin Shushan Zhao Liang Cheng Buhua Sun Ming Zeng Jianxi Zhu Ruibo Zhao Bangbao Lu Haitao Long Source Type: research

Current Perspectives on the Diagnosis and Management of Primary Urethral Cancer: A Systematic Review
Res Rep Urol. 2021 Jun 1;13:325-334. doi: 10.2147/RRU.S264720. eCollection 2021.ABSTRACTPrimary urethral cancer (PUC) is a rare but highly aggressive malignancy that causes malignant urethral obstruction. We conducted a literature review using PubMed to identify original research studies that assessed the diagnosis and management of primary urethral cancer. PUC affects men more than women, is more common in African Americans than Caucasians, and is associated with history of chronic inflammation and irritation of the urinary tract. Patients suspected of PUC should undergo a complete work-up including cystoscopy, magnetic r...
Source: Biomed Res - June 9, 2021 Category: Research Authors: M Ryan Farrell Jonathan T Xu Alex J Vanni Source Type: research