Integrated Analysis of Long Noncoding RNA Expression Profiles in Acute-on-Chronic Liver Failure
Biomed Res Int. 2021 May 18;2021:5387856. doi: 10.1155/2021/5387856. eCollection 2021.ABSTRACTPeople infected with chronic hepatitis B virus (HBV) might progress to acute-on-chronic liver failure (ACLF) with a high fatality rate. Long noncoding RNAs (lncRNAs) are involved in human diseases, but it is unknown whether lncRNAs are involved in the progression of chronic HBV infection to ACLF. Hence, this study is aimed at systemically identifying and characterizing the landscape and the molecular mechanism of lncRNAs in the pathogenesis of chronic HBV infection progress to ACLF. RNA sequencing (RNA-Seq) of peripheral blood sam...
Source: Biomed Res - June 9, 2021 Category: Research Authors: Xiaoyu Fu Da Cheng Yi Ouyang Ying Li Ronghua Li Shifang Peng Lei Fu Source Type: research

Identification of Molecular Characteristics and New Prognostic Targets for Thymoma by Multiomics Analysis
CONCLUSION: These results suggested that the molecular mechanism of thymoma may be related to immune inflammation. LIPE may be the key genes affecting prognosis of thymoma. Our findings will help to elucidate the pathogenesis and therapeutic targets of thymoma.PMID:34104648 | PMC:PMC8159640 | DOI:10.1155/2021/5587441 (Source: Biomed Res)
Source: Biomed Res - June 9, 2021 Category: Research Authors: Dazhong Liu Pengfei Zhang Jiaying Zhao Lei Yang Wei Wang Source Type: research

Interpreting the Molecular Mechanisms of Yinchenhao Decoction on Hepatocellular Carcinoma through Absorbed Components Based on Network Pharmacology
Biomed Res Int. 2021 May 19;2021:6616908. doi: 10.1155/2021/6616908. eCollection 2021.ABSTRACTTo investigate the mechanisms through which Yinchenhao decoction (YCHD) inhibits hepatocellular carcinoma (HCC), we analyzed YCHD ingredients absorbed into the bloodstream by using network pharmacology. We conducted a weighted gene coexpression network analysis on gene expression data collected from the Gene Expression Omnibus and The Cancer Genome Atlas databases to derive an HCC gene set; moreover, we used four online prediction system databases to predict the potential targets of YCHD ingredients absorbed into the bloodstream. ...
Source: Biomed Res - June 9, 2021 Category: Research Authors: Jijia Sun Tao Han Tao Yang Yunhui Chen Jihan Huang Source Type: research

Synergetic Action of Forskolin and Mevastatin Induce Normalization of Lipids Profile in Dyslipidemic Rats through Adenosine Monophosphate Kinase Upregulation
In conclusion, forskolin acts synergistically with mevastatin to lower lipid profile and improve lipid metabolism in dyslipidemic rats through upregulation of AMPK expression.PMID:34104650 | PMC:PMC8159628 | DOI:10.1155/2021/6687551 (Source: Biomed Res)
Source: Biomed Res - June 9, 2021 Category: Research Authors: Aaser M Abdelazim Tamer Ahmed Ismail Mosleh M Abumaghaid Islam M Saadaldin Source Type: research

The Prognostic Role of Prothrombin Time and Activated Partial Thromboplastin Time in Patients with Newly Diagnosed Multiple Myeloma
Biomed Res Int. 2021 May 19;2021:6689457. doi: 10.1155/2021/6689457. eCollection 2021.ABSTRACTPURPOSE: To evaluate the prognostic role of prothrombin time (PT) and activated partial thromboplastin time (APTT) for newly diagnosed multiple myeloma (MM).METHODS: We retrospectively analyzed 354 patients with newly diagnosed MM who received primary treatment in our center. The propensity score matching technique was used to reduce the bias between groups.RESULTS: Among 354 patients, lengthened PT or APTT was observed in 154 (43.5%) patients and 200 (56.5%) patients had normal PT and APTT. Patients with lengthened PT or APTT had...
Source: Biomed Res - June 9, 2021 Category: Research Authors: Chuanying Geng Guangzhong Yang Huijuan Wang Zhiyao Zhang Huixing Zhou Wenming Chen Source Type: research

Hemoglobin Level and Associated Factors among Pregnant Women in Rural Southwest Ethiopia
This study is aimed at assessing the hemoglobin level and associated factors among pregnant women in rural communities of Jimma zone, Southwest Ethiopia.METHODS: A community-based cross-sectional study design was carried out among 367 pregnant women from June 1 to 30, 2020. Systematic random sampling was used to select study subjects. Hemoglobin level was measured by using HemoCue HB 301. An interviewer-administered structured questionnaire was used to collect the data. Descriptive statistics were used to describe the study subjects. A multivariable linear regression model was employed after the linearity, normality, multi...
Source: Biomed Res - June 9, 2021 Category: Research Authors: Melesse Niguse Kuma Dessalegn Tamiru Tefera Belachew Source Type: research

High Neutrophil-to-Lymphocyte Ratio and Platelet-to-Lymphocyte Ratio Are Associated with Poor Survival in Patients with Hemodialysis
CONCLUSION: NLR and PLR are cheap and reliable biomarkers for all-cause and CV mortality to predict survival in HD patients.PMID:34104653 | PMC:PMC8159629 | DOI:10.1155/2021/9958081 (Source: Biomed Res)
Source: Biomed Res - June 9, 2021 Category: Research Authors: Jialing Zhang Xiangxue Lu Shixiang Wang Han Li Source Type: research

Hypofractionation and Stereotactic Body Radiation Therapy in Inoperable Locally Advanced Non-small Cell Lung Cancer
The objective of this article is to introduce professionals to the role that hypoRT and SBRT can play in the treatment of LA-NSCLC to offer the best treatment to their patients.PMID:34104822 | PMC:PMC8177839 (Source: Biomed Res)
Source: Biomed Res - June 9, 2021 Category: Research Authors: Mikel Rico Maribel Mart ínez Maitane Rodr íguez Lombardo Rosas Andrea Barco Enrique Mart ínez Source Type: research

Platelet and leukocyte count assay for thrombogenicity screening of biomaterials and medical devices: Evaluation and improvement of ASTM F2888 test standard
J Biomed Mater Res B Appl Biomater. 2021 Jun 9. doi: 10.1002/jbm.b.34887. Online ahead of print.ABSTRACTAn appropriate preclinical thrombogenicity evaluation of a blood-contacting device is important to reduce thrombosis and thromboembolism risks to patients. The in vitro platelet and leukocyte count assay, as described in the ASTM F2888 test standard, aims to assess thrombogenic potentials of blood-contacting materials. The goals of this study were to evaluate whether this standardized test method can effectively differentiate materials with different thrombogenic potentials and to investigate the impact of anticoagulatio...
Source: Biomed Res - June 9, 2021 Category: Research Authors: Qijin Lu Joshua M Nehrer Jiaming Li Megan A Jamiolkowski Jean E Rinaldi Richard A Malinauskas Source Type: research

Specific inhibition of α5 subunit-containing GABA < sub > A < /sub > receptors enhances locomotor activity and neuronal activity in the motor cortex
This study examined the effect of specific inhibition of α5 subunit-containing GABAA receptors (α5GABAAR) on the behavioral profile and neuronal activity of the CNS using a compound called L-655,708, which is a selective negative allosteric modulator of α5GABAAR. L-655,708 administration significantly increased locomotor activity without anxiety-related behavior. Furthermore, L-655,708 administration significantly increased c-Fos mRNA expression (a neuronal activity marker) in motor area of the cerebral cortex, whereas it hardly altered c-Fos mRNA expression in the sensory cortex, hippocampus, and spinal ...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Takahiro Inoue Yasuyuki Takamatsu Misato Okamura Hiroki Mani Naoya Hasegawa Hiroshi Maejima Source Type: research

Overexpressed exocyst complex component 3-like 1 spontaneously induces apoptosis
Biomed Res. 2021;42(3):109-113. doi: 10.2220/biomedres.42.109.ABSTRACTExocyst complex component 3-like 1 (EXOC3L1), which regulates insulin secretion, is ubiquitously present in heart, lung, liver, spleen, kidney, muscle, cerebellum, pituitary, adrenal grand, and pancreatic islets. Its deduced amino acid sequence has 31% identity and 53% similarity with Sec6, so they are considered isoforms. Since Sec6 suppresses apoptosis via HSP27, we investigated the involvement of EXOC3L1 expression in apoptosis. We found that overexpressed EXOC3L1 in Chinese hamster ovary cells significantly reduced cultured cell numbers. It also sign...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Shunichi Matsumoto Junichi Okada Eijiro Yamada Tsugumichi Saito Kazuya Okada Takuya Watanabe Yasuyo Nakajima Atsushi Ozawa Shuichi Okada Masanobu Yamada Source Type: research

Effects of aging on basement membrane-related gene expression of the skeletal muscle in rats
Biomed Res. 2021;42(3):115-119. doi: 10.2220/biomedres.42.115.ABSTRACTThe basement membrane (BM), with collagen IV as a major component, plays an important role in the maintenance of muscle structure and its robustness. To investigate the effects of aging on factors related to BM construction, we compared the expression status of these factors in 3- and 20-month-old male Wistar rats. The expression levels of Col4a1 and Col4a2 (encoding collagen IV), Sparc (involved in collagen IV functionalization), and Mmp14 (a collagen IV degradation factor) were decreased. These results suggest that aging suppresses collagen IV syntheti...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yuji Kanazawa Mamoru Nagano Satoshi Koinuma Shinichi Sugiyo Yasufumi Shigeyoshi Source Type: research

TBC1D8B, a GTPase-activating protein, is a novel apoptosis inducer
Biomed Res. 2021;42(3):95-102. doi: 10.2220/biomedres.42.95.ABSTRACTOverexpressed TBC1D8B, a GTPase-activating protein, significantly reduced cultured HCT116 human colon cancer cell number. We tested N-terminal TBC1D8B, which is identical to wild type TBC1D8B from amino acid positions 1 to 427 and possesses a modified sequence from position 428 to 435 (ECGGLFLL) because of the introduction of a premature stop codon at position 436 to narrow down the minimum requirement element. The N-terminal TBC1D8B contains two GRAM domains but not the TBC domain essential for Rab-GTPase activity. The N-terminal TBC1D8B overexpression si...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Junichi Okada Shunichi Matsumoto Eijiro Yamada Tsugumichi Saito Kazuya Okada Takuya Watanabe Yasuyo Nakajima Atsushi Ozawa Masanobu Yamada Kihachi Ohshima Shuichi Okada Source Type: research

The Role of Platelet-Rich Fibrin (PRF) in the Prevention of Medication-Related Osteonecrosis of the Jaw (MRONJ)
Biomed Res Int. 2021 May 13;2021:4948139. doi: 10.1155/2021/4948139. eCollection 2021.ABSTRACTDentoalveolar surgery is probably the major risk factor for MRONJ and for other complications following a tooth extraction, especially in patients affected by systemic diseases. The aim of this retrospective study is to evaluate whether a PRF plug inserted in the post extraction socket can prevent the onset of MRONJ. The patients were divided into two groups according to the surgical protocol that included the insertion or not of the PRF following the extraction and all the anamnestic, and clinical data were analyzed. In the contr...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Michele Miranda Francesco Gianfreda Carlo Raffone Donato Antonacci Valeria Pistilli Patrizio Bollero Source Type: research

Bacterial Pathogens and Their Antimicrobial Resistance Patterns of Inanimate Surfaces and Equipment in Ethiopia: A Systematic Review and Meta-analysis
CONCLUSION: This study has shown a high prevalence of bacterial contamination of inanimate surfaces and equipment in Ethiopia.PMID:34095296 | PMC:PMC8137297 | DOI:10.1155/2021/5519847 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Teklehaimanot Kiros Shewaneh Damtie Tahir Eyayu Tegenaw Tiruneh Wasihun Hailemichael Lemma Workineh Source Type: research

A Prediction Model Based on Noninvasive Indicators to Predict the 8-Year Incidence of Type 2 Diabetes in Patients with Nonalcoholic Fatty Liver Disease: A Population-Based Retrospective Cohort Study
CONCLUSIONS: We developed and validated a nomogram for the 8-year risk of incident T2D among Japanese patients with NAFLD. Our nomogram can effectively predict the 8-year incidence of T2D in Japanese patients with NAFLD and helps to identify people at high risk of T2D early, thus contributing to effective prevention programs for T2D.PMID:34095297 | PMC:PMC8140840 | DOI:10.1155/2021/5527460 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Xintian Cai Qing Zhu Yuanyuan Cao Shasha Liu Mengru Wang Ting Wu Jing Hong Ayguzal Ahmat Xiayire Aierken Nanfang Li Source Type: research

Promotion of Differentiating Bone Marrow Mesenchymal Stromal Cells (BMSCs) into Cardiomyocytes via HCN2 and HCN4 Cotransfection
CONCLUSION: HCN2 and HCN4 overexpression can considerably enhance the MSC ability to differentiate into cardiomyocytes in vitro and restore the ionic current.PMID:34095298 | PMC:PMC8140823 | DOI:10.1155/2021/5529276 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Xue Luo Hongxiao Li Xiaolin Sun Qisheng Zuo Bichun Li Ye Zhu Wei Wei Xiang Gu Source Type: research

Risk Factors for Gastric Cancer-Associated Thrombotic Diseases in a Han Chinese Population
Biomed Res Int. 2021 May 21;2021:5544188. doi: 10.1155/2021/5544188. eCollection 2021.ABSTRACTThe aim of the present work was to investigate the risk factors for gastric cancer- (GC-) associated thrombotic diseases in a Han Chinese population. A total of 333 patients diagnosed with GC, 68 with thrombotic diseases included in the case group and the remaining 265 in the control group, were enrolled. The relevant data for the participants, including general information (gender, age, smoking, and drinking), comorbidities (diabetes, hypertension, and anemia), tumor-related data (tumor site, histology, degree of differentiation,...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Bo Song Yamei Wang Xiuzhi Zhu Li Zhang Hui Zhou Hongmei Zhang Tianliang Zhang Wansheng Ji Source Type: research

The Accuracy of 16S rRNA Polymerase Chain Reaction for the Diagnosis of Neonatal Sepsis: A Meta-Analysis
CONCLUSIONS: The results from this meta-analysis indicate that 16S rRNA PCR testing is effective for the rapid diagnosis of neonatal sepsis.PMID:34095300 | PMC:PMC8140837 | DOI:10.1155/2021/5550387 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Ying Wang Jingyi Zhao Yinhui Yao Lan Yang Dan Zhao Shiquan Liu Source Type: research

Gentiopicroside Produces Endothelium-Independent Vasodilation by Deactivating the PI3K/Akt/Rho-Kinase Pathway in Isolated Rat Thoracic Aorta
Biomed Res Int. 2021 May 14;2021:5565748. doi: 10.1155/2021/5565748. eCollection 2021.ABSTRACTGentiopicroside (GPS), a main active secoiridoid glucoside derived from the roots of perennial herbs in the Gentianaceae family, has antispasmodic and relaxant effects. However, the vasorelaxant effects of GPS on aortic rings and the molecular mechanisms involved in these effects are not yet clear. Therefore, we investigated whether GPS inhibits phenylephrine- (PE-) or KCl-induced contractions in isolated rat thoracic aortic rings. The present study found that GPS produced a dose-dependent relaxation in aortic rings precontracted ...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Shangping Xing Feifei Nong Jialiang Qin Huicai Huang Ruoting Zhan Weiwen Chen Source Type: research

Effect of Deep Cervical Flexor Muscle Training Using Pressure Biofeedback on Pain and Forward Head Posture in School Teachers with Neck Pain: An Observational Study
CONCLUSIONS: This study shows that although pain and FHP improved following conventional exercises in school teachers with neck pain, mean improvement was more significant among those who received additional DCF muscle training using pressure biofeedback.PMID:34095302 | PMC:PMC8164544 | DOI:10.1155/2021/5588580 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Ahmad H Alghadir Zaheen A Iqbal Source Type: research

Mitophagy Impairment Aggravates Cisplatin-Induced Ototoxicity
In this study, HEI-OC1 cells were treated with either cisplatin alone (10 μM, 0, 8, 16, and 24 h); cisplatin (10 μM, 24 h) post transfection with small-interfering (si)RNAs targeting mitophagy-associated mRNAs; cisplatin (10 μM, 24 h) succeeding pretreatment with the mitophagy suppressor, 3-methyladenine (3-MA; 5 or 10 mM, 6 h); or cisplatin (30 μM, 24 h) following pretreatment with the mitophagy promoter, carbonyl cyanide m-chlorophenylhydrazone (CCCP; 1 or 2 μM, 2 h). The viability of cells, expression of mitophagy marker, and mitochondrial functions were then assessed in these cells. Cell viability was de...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Sung Il Cho Eu-Ri Jo Hansoo Song Source Type: research

Prognostic Value of Blood Urea Nitrogen/Creatinine Ratio for Septic Shock: An Analysis of the MIMIC-III Clinical Database
This study is aimed at determining the relationship between BCR and all-cause mortality in adult septic shock patients.METHODS: Data were extracted from the MIMIC-III database. The clinical endpoints were 28-, 90-, and 365-day all-cause mortality rates in critically ill septic shock patients. Cox proportional hazards models and subgroup analyses were used to analyze the relationship between BCR quartiles and all-cause mortality in septic shock patients. Receiver operator characteristic (ROC) curves and areas under the ROC curves (AUCs) were calculated to evaluate how accurately BCR predicts the mortality of septic shock pa...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Didi Han Luming Zhang Shuai Zheng Fengshuo Xu Chengzhuo Li Rui Yang Wen Ma Haiyan Yin Jun Lyu Source Type: research

A Combination of Geniposide and Chlorogenic Acid Combination Ameliorates Nonalcoholic Steatohepatitis in Mice by Inhibiting Kupffer Cell Activation
Biomed Res Int. 2021 May 13;2021:6615881. doi: 10.1155/2021/6615881. eCollection 2021.ABSTRACTThe incidence of nonalcoholic steatohepatitis (NASH) is increasing worldwide. Activation of Kupffer cells (KCs) is central to the development of diet-induced NASH. We investigated whether a combination of two active chemical components, geniposide and chlorogenic acid (GC), at a specific ratio (67 : 1), ameliorates diet-induced NASH and the underlying mechanisms involved. C57BL/6J mice exposed to a high-fat and high-cholesterol (HFHC) diet containing cholesterol, choline, and high-sugar drinking water, as well as RAW264.7 cells st...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Xin Xin Yue Jin Xin Wang Beiyu Cai Ziming An Yi-Yang Hu Qin Feng Source Type: research

Genome-Wide Screening Identifies Prognostic Long Noncoding RNAs in Hepatocellular Carcinoma
Biomed Res Int. 2021 May 20;2021:6640652. doi: 10.1155/2021/6640652. eCollection 2021.ABSTRACTHepatocellular carcinoma (HCC) is a common malignancy with a poor prognosis. Therefore, there is an urgent call for the investigation of novel biomarkers in HCC. In the present study, we identified 6 upregulated lncRNAs in HCC, including LINC01134, RHPN1-AS1, NRAV, CMB9-22P13.1, MKLN1-AS, and MAPKAPK5-AS1. Higher expression of these lncRNAs was correlated to a more advanced cancer stage and a poorer prognosis in HCC patients. Enrichment analysis revealed that these lncRNAs played a crucial role in HCC progression, possibly through...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yujie Feng Xiao Hu Kai Ma Bingyuan Zhang Chuandong Sun Source Type: research

Utilization of Deworming Drugs and Its Individual and Community Level Predictors among Pregnant Married Women in Cameroon: A Multilevel Modeling
CONCLUSION: Access to education and economic empowerment of pregnant married women in remote areas and the north region should be the primary focus of the Cameroon government to enhance deworming coverage in the country.PMID:34095307 | PMC:PMC8137305 | DOI:10.1155/2021/6645336 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Betregiorgis Zegeye Bright Opoku Ahinkorah Edward Kwabena Ameyaw Abdul-Aziz Seidu Sanni Yaya Source Type: research

Virtual Screening of Phytochemicals by Targeting HR1 Domain of SARS-CoV-2 S Protein: Molecular Docking, Molecular Dynamics Simulations, and DFT Studies
Biomed Res Int. 2021 May 20;2021:6661191. doi: 10.1155/2021/6661191. eCollection 2021.ABSTRACTThe recent COVID-19 pandemic has impacted nearly the whole world due to its high morbidity and mortality rate. Thus, scientists around the globe are working to find potent drugs and designing an effective vaccine against COVID-19. Phytochemicals from medicinal plants are known to have a long history for the treatment of various pathogens and infections; thus, keeping this in mind, this study was performed to explore the potential of different phytochemicals as candidate inhibitors of the HR1 domain in SARS-CoV-2 spike protein by u...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Arshia Majeed Waqar Hussain Farkhanda Yasmin Ammara Akhtar Nouman Rasool Source Type: research

Preoperative Nutrition Support May Reduce the Prevalence of Postoperative Pancreatic Fistula after Open Pancreaticoduodenectomy in Patients with High Nutritional Risk Determined by NRS2002
CONCLUSIONS: Patients with high nutritional risk (NRS2002 score ≥ 5) may profit from preoperative nutritional support manifested in the reduction of CR-POPF.PMID:34095309 | PMC:PMC8163530 | DOI:10.1155/2021/6691966 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Jing-Yong Xu Xiao-Dong Tian Jing-Hai Song Jian Chen Yin-Mo Yang Jun-Min Wei Source Type: research

Decreased Levels of Serum IL-34 Associated with Cognitive Impairment in Vascular Dementia
Biomed Res Int. 2021 May 21;2021:6793860. doi: 10.1155/2021/6793860. eCollection 2021.ABSTRACTOBJECTIVE: Interleukin- (IL-) 34 is a new type of cytokine with neuroprotective effects discovered in recent years. However, the relationship between IL-34 and vascular dementia (VaD) has not yet been elucidated. The purpose of this study is to determine whether IL-34 is involved in cognitive impairment of VaD.METHODS: From January 2017 to December 2020, 84 VaD patients and 60 healthy controls who attended Qingpu Branch of Zhongshan Hospital were prospectively included in the study. Once included in the study, demographic features...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yang Wang Wei Lu Wenjing Ning Yan Chen Lingxing Li Source Type: research

Ageing Effects, Generation Means, and Path Coefficient Analyses on High Kernel Elongation in Mahsuri Mutan and Basmati 370 Rice Populations
The objectives of this study were to determine the effects of ageing treatments, gene actions, and inheritance pattern of kernel elongation on cooking quality in two populations of rice and determine the path of influence and contribution of other traits to kernel elongation in rice. Two rice populations derived from crosses between MR219 × Mahsuri Mutan and MR219 × Basmati 370 were used. The breeding materials included two F1 progenies from the two populations, and their respective parents were grown in four different batches at a week interval to synchronize the flowering between the female and male plants. S...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Anna Arina Bt Ab Halim Mohd Y Rafii Mohamad B Osman Yusuff Oladosu Samuel C Chukwu Source Type: research

A Chimera of Th1 Stimulatory Proteins of < em > Leishmania donovani < /em > Offers Moderate Immunotherapeutic Efficacy with a Th1-Inclined Immune Response against Visceral Leishmaniasis
Biomed Res Int. 2021 Feb 27;2021:8845826. doi: 10.1155/2021/8845826. eCollection 2021.ABSTRACTImmunotherapy, a treatment based on host immune system activation, has been shown to provide a substitute for marginally effective conventional chemotherapy in controlling visceral leishmaniasis (VL), the deadliest form of leishmaniasis. As the majority of endemic inhabitants exhibit either subclinical or asymptomatic infection which often develops into the active disease state, therapeutic intervention seems to be an important avenue for combating infections by stimulating the natural defense system of infected individuals. With ...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Sneha Ratnapriya None Keerti Narendra Kumar Yadav Anuradha Dube Amogh Anant Sahasrabuddhe Source Type: research

Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells
CONCLUSION: This study shows that curcumin simultaneously induces apoptosis and necroptosis by oxidative mitochondrial dysfunction and subsequent ATP depletion, providing a mechanistic basis for understanding the novel role of curcumin for prostate carcinoma cells.PMID:34095313 | PMC:PMC8164543 | DOI:10.1155/2021/8859181 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yoon-Jin Lee Kwan-Sik Park Sang-Han Lee Source Type: research

Cell Heterogeneity Analysis in Single-Cell RNA-seq Data Using Mixture Exponential Graph and Markov Random Field Model
Biomed Res Int. 2021 May 22;2021:9919080. doi: 10.1155/2021/9919080. eCollection 2021.ABSTRACTAdvanced single-cell profiling technologies promote exploration of cell heterogeneity, and clustering of single-cell RNA (scRNA-seq) data enables discovery of coexpression genes and network relationships between genes. In particular, single-cell profiling of circulating tumor cells (CTCs) can provide unique insights into tumor heterogeneity (including in triple-negative breast cancer (TNBC)), while scRNA-seq leads to better understanding of subclonal architecture and biological function. Despite numerous reports suggesting a direc...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yishu Wang Xuehan Tian Dongmei Ai Source Type: research

Quality of Life in Adult Patients Receiving Cervical Fusion for Fresh Subaxial Cervical Injury: The Role of Associated Spinal Cord Injury
CONCLUSION: At the final observation, HRQOL scores were lower in patients with SCI than in their non-SCI counterparts, obviously because of the associated neurologic impairment. SF-36 scores correlated with several sagittal roentgenographic parameters. These correlations should be taken in consideration by spine surgeons when performing cervical spine surgery for fresh cervical spine injuries.PMID:34095315 | PMC:PMC8140844 | DOI:10.1155/2021/9931535 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Panagiotis Korovessis Evangelia Mpountogianni Vasileios Syrimpeis Maria Andriopoulou Alkis Korovesis Source Type: research

Peptidome Analysis of Pancreatic Tissue Derived from T1DM Mice: Insights into the Pathogenesis and Clinical Treatments of T1DM
In conclusion, this study highlights new candidate peptides and provides a new perspective for exploring T1DM pathogenesis and clinical treatments.PMID:34095316 | PMC:PMC8164536 | DOI:10.1155/2021/9987042 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Fan Zhang Meiyun Zhou Shuangshuang Li Jinhua Gu Yuanyuan Qian Sisi He Li Hong Linlin Sun Xiaohua Zhang Weigang Ji Source Type: research

Laser-induced cavitation in plasmonic nanoparticle solutions: A comparative study between gold and titanium nitride
This study advances our understanding of the optical properties of TiN and discusses significant differences compared to gold, with important implications for future applications of this material in water treatment, nonlinear signal converting, and laser-induced cavitation for medical implementations, among others.PMID:34096159 | DOI:10.1002/jbm.a.37242 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Ariana Nushin Sabzeghabae Carla Berrospe-Rodriguez Lorenzo Mangolini Guillermo Aguilar Source Type: research

The performance of heparin modified poly( ε-caprolactone) small diameter tissue engineering vascular graft in canine-A long-term pilot experiment in vivo
In this study, a SDTEVG is fabricated by the electrospinning of poly(ε-caprolactone) and subsequent heparin modification. SDTEVG is implanted into canine's abdominal aorta for 511 days in order to investigate its clinical feasibility. An active and robust remodeling process was characterized by a confluent endothelium, macrophage infiltrate, extracellular matrix deposition and remodeling on the explanted graft. The immunohistochemical and immunofluorescence analysis further exhibit the regeneration of endothelium and smooth muscle layer on tunica intima and tunica media, respectively. Thus, long-term follow-up reve...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Lin Ye Toshitaka Takagi Chengzhao Tu Akeo Hagiwara Xue Geng Zengguo Feng Source Type: research

Specific inhibition of α5 subunit-containing GABA < sub > A < /sub > receptors enhances locomotor activity and neuronal activity in the motor cortex
This study examined the effect of specific inhibition of α5 subunit-containing GABAA receptors (α5GABAAR) on the behavioral profile and neuronal activity of the CNS using a compound called L-655,708, which is a selective negative allosteric modulator of α5GABAAR. L-655,708 administration significantly increased locomotor activity without anxiety-related behavior. Furthermore, L-655,708 administration significantly increased c-Fos mRNA expression (a neuronal activity marker) in motor area of the cerebral cortex, whereas it hardly altered c-Fos mRNA expression in the sensory cortex, hippocampus, and spinal ...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Takahiro Inoue Yasuyuki Takamatsu Misato Okamura Hiroki Mani Naoya Hasegawa Hiroshi Maejima Source Type: research

Overexpressed exocyst complex component 3-like 1 spontaneously induces apoptosis
Biomed Res. 2021;42(3):109-113. doi: 10.2220/biomedres.42.109.ABSTRACTExocyst complex component 3-like 1 (EXOC3L1), which regulates insulin secretion, is ubiquitously present in heart, lung, liver, spleen, kidney, muscle, cerebellum, pituitary, adrenal grand, and pancreatic islets. Its deduced amino acid sequence has 31% identity and 53% similarity with Sec6, so they are considered isoforms. Since Sec6 suppresses apoptosis via HSP27, we investigated the involvement of EXOC3L1 expression in apoptosis. We found that overexpressed EXOC3L1 in Chinese hamster ovary cells significantly reduced cultured cell numbers. It also sign...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Shunichi Matsumoto Junichi Okada Eijiro Yamada Tsugumichi Saito Kazuya Okada Takuya Watanabe Yasuyo Nakajima Atsushi Ozawa Shuichi Okada Masanobu Yamada Source Type: research

Effects of aging on basement membrane-related gene expression of the skeletal muscle in rats
Biomed Res. 2021;42(3):115-119. doi: 10.2220/biomedres.42.115.ABSTRACTThe basement membrane (BM), with collagen IV as a major component, plays an important role in the maintenance of muscle structure and its robustness. To investigate the effects of aging on factors related to BM construction, we compared the expression status of these factors in 3- and 20-month-old male Wistar rats. The expression levels of Col4a1 and Col4a2 (encoding collagen IV), Sparc (involved in collagen IV functionalization), and Mmp14 (a collagen IV degradation factor) were decreased. These results suggest that aging suppresses collagen IV syntheti...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Yuji Kanazawa Mamoru Nagano Satoshi Koinuma Shinichi Sugiyo Yasufumi Shigeyoshi Source Type: research

TBC1D8B, a GTPase-activating protein, is a novel apoptosis inducer
Biomed Res. 2021;42(3):95-102. doi: 10.2220/biomedres.42.95.ABSTRACTOverexpressed TBC1D8B, a GTPase-activating protein, significantly reduced cultured HCT116 human colon cancer cell number. We tested N-terminal TBC1D8B, which is identical to wild type TBC1D8B from amino acid positions 1 to 427 and possesses a modified sequence from position 428 to 435 (ECGGLFLL) because of the introduction of a premature stop codon at position 436 to narrow down the minimum requirement element. The N-terminal TBC1D8B contains two GRAM domains but not the TBC domain essential for Rab-GTPase activity. The N-terminal TBC1D8B overexpression si...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Junichi Okada Shunichi Matsumoto Eijiro Yamada Tsugumichi Saito Kazuya Okada Takuya Watanabe Yasuyo Nakajima Atsushi Ozawa Masanobu Yamada Kihachi Ohshima Shuichi Okada Source Type: research

The Role of Platelet-Rich Fibrin (PRF) in the Prevention of Medication-Related Osteonecrosis of the Jaw (MRONJ)
Biomed Res Int. 2021 May 13;2021:4948139. doi: 10.1155/2021/4948139. eCollection 2021.ABSTRACTDentoalveolar surgery is probably the major risk factor for MRONJ and for other complications following a tooth extraction, especially in patients affected by systemic diseases. The aim of this retrospective study is to evaluate whether a PRF plug inserted in the post extraction socket can prevent the onset of MRONJ. The patients were divided into two groups according to the surgical protocol that included the insertion or not of the PRF following the extraction and all the anamnestic, and clinical data were analyzed. In the contr...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Michele Miranda Francesco Gianfreda Carlo Raffone Donato Antonacci Valeria Pistilli Patrizio Bollero Source Type: research

Bacterial Pathogens and Their Antimicrobial Resistance Patterns of Inanimate Surfaces and Equipment in Ethiopia: A Systematic Review and Meta-analysis
CONCLUSION: This study has shown a high prevalence of bacterial contamination of inanimate surfaces and equipment in Ethiopia.PMID:34095296 | PMC:PMC8137297 | DOI:10.1155/2021/5519847 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Teklehaimanot Kiros Shewaneh Damtie Tahir Eyayu Tegenaw Tiruneh Wasihun Hailemichael Lemma Workineh Source Type: research

A Prediction Model Based on Noninvasive Indicators to Predict the 8-Year Incidence of Type 2 Diabetes in Patients with Nonalcoholic Fatty Liver Disease: A Population-Based Retrospective Cohort Study
CONCLUSIONS: We developed and validated a nomogram for the 8-year risk of incident T2D among Japanese patients with NAFLD. Our nomogram can effectively predict the 8-year incidence of T2D in Japanese patients with NAFLD and helps to identify people at high risk of T2D early, thus contributing to effective prevention programs for T2D.PMID:34095297 | PMC:PMC8140840 | DOI:10.1155/2021/5527460 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Xintian Cai Qing Zhu Yuanyuan Cao Shasha Liu Mengru Wang Ting Wu Jing Hong Ayguzal Ahmat Xiayire Aierken Nanfang Li Source Type: research

Promotion of Differentiating Bone Marrow Mesenchymal Stromal Cells (BMSCs) into Cardiomyocytes via HCN2 and HCN4 Cotransfection
CONCLUSION: HCN2 and HCN4 overexpression can considerably enhance the MSC ability to differentiate into cardiomyocytes in vitro and restore the ionic current.PMID:34095298 | PMC:PMC8140823 | DOI:10.1155/2021/5529276 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Xue Luo Hongxiao Li Xiaolin Sun Qisheng Zuo Bichun Li Ye Zhu Wei Wei Xiang Gu Source Type: research

Risk Factors for Gastric Cancer-Associated Thrombotic Diseases in a Han Chinese Population
Biomed Res Int. 2021 May 21;2021:5544188. doi: 10.1155/2021/5544188. eCollection 2021.ABSTRACTThe aim of the present work was to investigate the risk factors for gastric cancer- (GC-) associated thrombotic diseases in a Han Chinese population. A total of 333 patients diagnosed with GC, 68 with thrombotic diseases included in the case group and the remaining 265 in the control group, were enrolled. The relevant data for the participants, including general information (gender, age, smoking, and drinking), comorbidities (diabetes, hypertension, and anemia), tumor-related data (tumor site, histology, degree of differentiation,...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Bo Song Yamei Wang Xiuzhi Zhu Li Zhang Hui Zhou Hongmei Zhang Tianliang Zhang Wansheng Ji Source Type: research

The Accuracy of 16S rRNA Polymerase Chain Reaction for the Diagnosis of Neonatal Sepsis: A Meta-Analysis
CONCLUSIONS: The results from this meta-analysis indicate that 16S rRNA PCR testing is effective for the rapid diagnosis of neonatal sepsis.PMID:34095300 | PMC:PMC8140837 | DOI:10.1155/2021/5550387 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Ying Wang Jingyi Zhao Yinhui Yao Lan Yang Dan Zhao Shiquan Liu Source Type: research

Gentiopicroside Produces Endothelium-Independent Vasodilation by Deactivating the PI3K/Akt/Rho-Kinase Pathway in Isolated Rat Thoracic Aorta
Biomed Res Int. 2021 May 14;2021:5565748. doi: 10.1155/2021/5565748. eCollection 2021.ABSTRACTGentiopicroside (GPS), a main active secoiridoid glucoside derived from the roots of perennial herbs in the Gentianaceae family, has antispasmodic and relaxant effects. However, the vasorelaxant effects of GPS on aortic rings and the molecular mechanisms involved in these effects are not yet clear. Therefore, we investigated whether GPS inhibits phenylephrine- (PE-) or KCl-induced contractions in isolated rat thoracic aortic rings. The present study found that GPS produced a dose-dependent relaxation in aortic rings precontracted ...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Shangping Xing Feifei Nong Jialiang Qin Huicai Huang Ruoting Zhan Weiwen Chen Source Type: research

Effect of Deep Cervical Flexor Muscle Training Using Pressure Biofeedback on Pain and Forward Head Posture in School Teachers with Neck Pain: An Observational Study
CONCLUSIONS: This study shows that although pain and FHP improved following conventional exercises in school teachers with neck pain, mean improvement was more significant among those who received additional DCF muscle training using pressure biofeedback.PMID:34095302 | PMC:PMC8164544 | DOI:10.1155/2021/5588580 (Source: Biomed Res)
Source: Biomed Res - June 7, 2021 Category: Research Authors: Ahmad H Alghadir Zaheen A Iqbal Source Type: research

Mitophagy Impairment Aggravates Cisplatin-Induced Ototoxicity
In this study, HEI-OC1 cells were treated with either cisplatin alone (10 μM, 0, 8, 16, and 24 h); cisplatin (10 μM, 24 h) post transfection with small-interfering (si)RNAs targeting mitophagy-associated mRNAs; cisplatin (10 μM, 24 h) succeeding pretreatment with the mitophagy suppressor, 3-methyladenine (3-MA; 5 or 10 mM, 6 h); or cisplatin (30 μM, 24 h) following pretreatment with the mitophagy promoter, carbonyl cyanide m-chlorophenylhydrazone (CCCP; 1 or 2 μM, 2 h). The viability of cells, expression of mitophagy marker, and mitochondrial functions were then assessed in these cells. Cell viability was de...
Source: Biomed Res - June 7, 2021 Category: Research Authors: Sung Il Cho Eu-Ri Jo Hansoo Song Source Type: research