Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37705. Online ahead of print.ABSTRACTRNA-based therapeutics have gained traction for the prevention and treatment of a variety of diseases. However, their fragility and immunogenicity necessitate a drug carrier. Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing evidence that the structure of IL lipid tails significantly impacts the e...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Kaitlin Mrksich Marshall S Padilla Ryann A Joseph Emily L Han Dongyoon Kim Rohan Palanki Junchao Xu Michael J Mitchell Source Type: research
Mesenchymal stem cell-secretome laden photopolymerizable hydrogels for wound healing
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37697. Online ahead of print.ABSTRACTMesenchymal stem cell-derived secretome represents an emerging acellular therapeutic which possess significant opportunity for clinical applications due to its anti-inflammatory, immunomodulatory, and wound healing properties. However, maintaining therapeutic efficacy and ensuring stability of cell-based products is challenging, requiring a robust delivery method. Therefore, we designed a hydrogel-based scaffold loaded with CK Cell Technologies' proprietary Mesenchymal stem cell-secretome for controlled release treatment of acute and...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Riddhesh B Doshi Devashree Vakil Thomas G Molley Md Shariful Islam Kristopher A Kilian Corey Cunningham Kuldip S Sidhu Source Type: research
pO < sub > 2 < /sub > reporter composite hydrogel macroencapsulation devices for magnetic resonance imaging oxygen quantification
In this study, we engineer pO2 reporter composite hydrogels (PORCH) to enable spatiotemporal measurement of oxygen tension within macroencapsulation devices using the proton Imaging of siloxanes to map tissue oxygenation levels (PISTOL) magnetic resonance imaging approach. We engineer two methods of incorporating siloxane oximetry reporters within hydrogel devices, an emulsion and microbead-based approach, and evaluate PORCH cytotoxicity on co-encapsulated cells and accuracy in quantifying oxygen tension in vitro. We find that both emulsion and microbead PORCH approaches enable accurate in situ oxygen quantification using ...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Amy E Emerson Yuka Sugamura Jad Mazboudi Tuhfah M Abdallah Charmayne D Seaton Azin Ghasemi Vikram D Kodibagkar Jessica D Weaver Source Type: research
A nontoxic strontium nanoparticle that holds the potential to act upon osteocompetent cells: An in vitro and in vivo characterization
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37708. Online ahead of print.ABSTRACTEstrogen deficiency, long-term immobilization, and/or aging are commonly related to bone mass loss, thus increasing the risk of fractures. One option for bone replacement in injuries caused by either traumas or pathologies is the use of orthopedic cement based on polymethylmethacrylate (PMMA). Nevertheless, its reduced bioactivity may induce long-term detachment from the host tissue, resulting in the failure of the implant. In view of this problem, we developed an alternative PMMA-based porous cement (pPMMA) that favors cell invasion...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Larwsk Hayann Vitor Freire da Rocha Marina Ferreira C ândido Raphael Martini Vicente Luiz H S Andrilli Sandra Y Fukada Mar ía Sol Brassesco Pietro Ciancaglini Edgard Eduard Engel Ana Paula Ramos Source Type: research
Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37705. Online ahead of print.ABSTRACTRNA-based therapeutics have gained traction for the prevention and treatment of a variety of diseases. However, their fragility and immunogenicity necessitate a drug carrier. Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing evidence that the structure of IL lipid tails significantly impacts the e...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Kaitlin Mrksich Marshall S Padilla Ryann A Joseph Emily L Han Dongyoon Kim Rohan Palanki Junchao Xu Michael J Mitchell Source Type: research
Mesenchymal stem cell-secretome laden photopolymerizable hydrogels for wound healing
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37697. Online ahead of print.ABSTRACTMesenchymal stem cell-derived secretome represents an emerging acellular therapeutic which possess significant opportunity for clinical applications due to its anti-inflammatory, immunomodulatory, and wound healing properties. However, maintaining therapeutic efficacy and ensuring stability of cell-based products is challenging, requiring a robust delivery method. Therefore, we designed a hydrogel-based scaffold loaded with CK Cell Technologies' proprietary Mesenchymal stem cell-secretome for controlled release treatment of acute and...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Riddhesh B Doshi Devashree Vakil Thomas G Molley Md Shariful Islam Kristopher A Kilian Corey Cunningham Kuldip S Sidhu Source Type: research
pO < sub > 2 < /sub > reporter composite hydrogel macroencapsulation devices for magnetic resonance imaging oxygen quantification
In this study, we engineer pO2 reporter composite hydrogels (PORCH) to enable spatiotemporal measurement of oxygen tension within macroencapsulation devices using the proton Imaging of siloxanes to map tissue oxygenation levels (PISTOL) magnetic resonance imaging approach. We engineer two methods of incorporating siloxane oximetry reporters within hydrogel devices, an emulsion and microbead-based approach, and evaluate PORCH cytotoxicity on co-encapsulated cells and accuracy in quantifying oxygen tension in vitro. We find that both emulsion and microbead PORCH approaches enable accurate in situ oxygen quantification using ...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Amy E Emerson Yuka Sugamura Jad Mazboudi Tuhfah M Abdallah Charmayne D Seaton Azin Ghasemi Vikram D Kodibagkar Jessica D Weaver Source Type: research
A nontoxic strontium nanoparticle that holds the potential to act upon osteocompetent cells: An in vitro and in vivo characterization
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37708. Online ahead of print.ABSTRACTEstrogen deficiency, long-term immobilization, and/or aging are commonly related to bone mass loss, thus increasing the risk of fractures. One option for bone replacement in injuries caused by either traumas or pathologies is the use of orthopedic cement based on polymethylmethacrylate (PMMA). Nevertheless, its reduced bioactivity may induce long-term detachment from the host tissue, resulting in the failure of the implant. In view of this problem, we developed an alternative PMMA-based porous cement (pPMMA) that favors cell invasion...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Larwsk Hayann Vitor Freire da Rocha Marina Ferreira C ândido Raphael Martini Vicente Luiz H S Andrilli Sandra Y Fukada Mar ía Sol Brassesco Pietro Ciancaglini Edgard Eduard Engel Ana Paula Ramos Source Type: research
Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37705. Online ahead of print.ABSTRACTRNA-based therapeutics have gained traction for the prevention and treatment of a variety of diseases. However, their fragility and immunogenicity necessitate a drug carrier. Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing evidence that the structure of IL lipid tails significantly impacts the e...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Kaitlin Mrksich Marshall S Padilla Ryann A Joseph Emily L Han Dongyoon Kim Rohan Palanki Junchao Xu Michael J Mitchell Source Type: research
Mesenchymal stem cell-secretome laden photopolymerizable hydrogels for wound healing
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37697. Online ahead of print.ABSTRACTMesenchymal stem cell-derived secretome represents an emerging acellular therapeutic which possess significant opportunity for clinical applications due to its anti-inflammatory, immunomodulatory, and wound healing properties. However, maintaining therapeutic efficacy and ensuring stability of cell-based products is challenging, requiring a robust delivery method. Therefore, we designed a hydrogel-based scaffold loaded with CK Cell Technologies' proprietary Mesenchymal stem cell-secretome for controlled release treatment of acute and...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Riddhesh B Doshi Devashree Vakil Thomas G Molley Md Shariful Islam Kristopher A Kilian Corey Cunningham Kuldip S Sidhu Source Type: research
pO < sub > 2 < /sub > reporter composite hydrogel macroencapsulation devices for magnetic resonance imaging oxygen quantification
In this study, we engineer pO2 reporter composite hydrogels (PORCH) to enable spatiotemporal measurement of oxygen tension within macroencapsulation devices using the proton Imaging of siloxanes to map tissue oxygenation levels (PISTOL) magnetic resonance imaging approach. We engineer two methods of incorporating siloxane oximetry reporters within hydrogel devices, an emulsion and microbead-based approach, and evaluate PORCH cytotoxicity on co-encapsulated cells and accuracy in quantifying oxygen tension in vitro. We find that both emulsion and microbead PORCH approaches enable accurate in situ oxygen quantification using ...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Amy E Emerson Yuka Sugamura Jad Mazboudi Tuhfah M Abdallah Charmayne D Seaton Azin Ghasemi Vikram D Kodibagkar Jessica D Weaver Source Type: research
A nontoxic strontium nanoparticle that holds the potential to act upon osteocompetent cells: An in vitro and in vivo characterization
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37708. Online ahead of print.ABSTRACTEstrogen deficiency, long-term immobilization, and/or aging are commonly related to bone mass loss, thus increasing the risk of fractures. One option for bone replacement in injuries caused by either traumas or pathologies is the use of orthopedic cement based on polymethylmethacrylate (PMMA). Nevertheless, its reduced bioactivity may induce long-term detachment from the host tissue, resulting in the failure of the implant. In view of this problem, we developed an alternative PMMA-based porous cement (pPMMA) that favors cell invasion...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Larwsk Hayann Vitor Freire da Rocha Marina Ferreira C ândido Raphael Martini Vicente Luiz H S Andrilli Sandra Y Fukada Mar ía Sol Brassesco Pietro Ciancaglini Edgard Eduard Engel Ana Paula Ramos Source Type: research
Influence of ionizable lipid tail length on lipid nanoparticle delivery of mRNA of varying length
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37705. Online ahead of print.ABSTRACTRNA-based therapeutics have gained traction for the prevention and treatment of a variety of diseases. However, their fragility and immunogenicity necessitate a drug carrier. Lipid nanoparticles (LNPs) have emerged as the predominant delivery vehicle for RNA therapeutics. An important component of LNPs is the ionizable lipid (IL), which is protonated in the acidic environment of the endosome, prompting cargo release into the cytosol. Currently, there is growing evidence that the structure of IL lipid tails significantly impacts the e...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Kaitlin Mrksich Marshall S Padilla Ryann A Joseph Emily L Han Dongyoon Kim Rohan Palanki Junchao Xu Michael J Mitchell Source Type: research
Mesenchymal stem cell-secretome laden photopolymerizable hydrogels for wound healing
J Biomed Mater Res A. 2024 Mar 15. doi: 10.1002/jbm.a.37697. Online ahead of print.ABSTRACTMesenchymal stem cell-derived secretome represents an emerging acellular therapeutic which possess significant opportunity for clinical applications due to its anti-inflammatory, immunomodulatory, and wound healing properties. However, maintaining therapeutic efficacy and ensuring stability of cell-based products is challenging, requiring a robust delivery method. Therefore, we designed a hydrogel-based scaffold loaded with CK Cell Technologies' proprietary Mesenchymal stem cell-secretome for controlled release treatment of acute and...
Source: Biomed Res - March 15, 2024 Category: Research Authors: Riddhesh B Doshi Devashree Vakil Thomas G Molley Md Shariful Islam Kristopher A Kilian Corey Cunningham Kuldip S Sidhu Source Type: research
Extrusion 3D-printed tricalcium phosphate-polycaprolactone biocomposites for quercetin-KCl delivery in bone tissue engineering
This study provides valuable insights as a promising strategy for bone tissue engineering (BTE) in orthopedic repair.PMID:38477071 | DOI:10.1002/jbm.a.37692 (Source: Biomed Res)
Source: Biomed Res - March 13, 2024 Category: Research Authors: Connor Toulou Vishal Sharad Chaudhari Susmita Bose Source Type: research
