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Condition: Rheumatoid Arthritis
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Total 292 results found since Jan 2013.
Histone deacetylase 1 regulates tissue destruction in rheumatoid arthritis
In this study, we have investigated the role of histone deacetylase (HDAC) enzymes in RA synovial fibroblasts (RASFs), a key cellular mediator of cartilage and bone destruction and determined effects of HDAC1 inhibition on both RASF phenotype in vitro, and joint inflammation and damage in the collagen-induced arthritis (CIA) model. Expression of HDACs 1–11 messenger ribonucleic acid (mRNA) was compared between RASFs and osteoarthritic synovial fibroblast (OASFs) using quantitative polymerase chain reaction. HDAC1 expression in RASFs was inhibited using small interfering RNA (siRNA) technology to assess effects on inv...
Source: Human Molecular Genetics - September 10, 2015 Category: Genetics & Stem Cells Authors: Hawtree, S., Muthana, M., Wilkinson, J. M., Akil, M., Wilson, A. G. Tags: ORIGINAL ARTICLES Source Type: research
Inhibition of G-Protein βγ Signaling Decreases Levels of Messenger RNAs Encoding Proinflammatory Cytokines in T Cell Receptor-Stimulated CD4+ T Helper Cells
Conclusions: Inhibiting Gβγ to produce these shifts in cytokine mRNA production might be beneficial for patients with autoimmune diseases such as rheumatoid arthritis (RA), Crohn’s disease (CD), psoriasis, multiple sclerosis (MS), and Hashimoto’s thyroiditis (HT), in which both IFN-γ and IL-17A are elevated. Published on None
Source: Journal of Molecular Signaling - August 21, 2015 Category: Molecular Biology Source Type: research
Inhibition of smoothened decreases proliferation of synoviocytes in rheumatoid arthritis.
The objective of this study was to investigate the role of Smo in RA synoviocyte proliferation. FLSs were isolated from RA synovium. Shh signaling was studied using a Smo antagonist (GDC-0449) and small interfering RNA (siRNA) targeting the Smo gene in FLSs. Cell proliferation was quantified by using kit-8 assay and cell cycle distribution and apoptosis were evaluated by flow cytometry. Cell cycle-related genes and proteins were detected by real-time PCR and western blot. FLSs treated with GDC-0449 or Smo-siRNA showed significantly decreased proliferation compared to controls (P < 0.05). Incubation with GDC-0449 or tran...
Source: Cellular and Molecular Immunology - July 20, 2015 Category: Molecular Biology Authors: Zhu SL, Huang JL, Peng WX, Wu DC, Luo MQ, Li QX, Li ZX, Feng XX, Liu F, Wang MX, Chen WQ, Olsen N, Zheng SG Tags: Cell Mol Immunol Source Type: research
Monocarboxylate transporter 4, associated with the acidification of synovial fluid, is a novel therapeutic target for inflammatory arthritis
Conclusion. RA activity correlated with decreased synovial fluid pH. This may be due to increased MCT4 expression in RASFs. Silencing MCT4 induced apoptosis in RASFs and reduced the severity of CIA, suggesting that MCT4 is a potential therapeutic target for inflammatory arthritis. This article is protected by copyright. All rights reserved.
Source: Arthritis and Rheumatism - July 20, 2015 Category: Rheumatology Authors: Wataru Fujii, Yutaka Kawahito, Hidetake Nagahara, Yuji Kukida, Takahiro Seno, Aihiro Yamamoto, Masataka Kohno, Ryo Oda, Daigo Taniguchi, Hiroyoshi Fujiwara, Akika Ejima, Tsunao Kishida, Osam Mazda, Eishi Ashihara Tags: Full Length Source Type: research
Knockdown of ADAM10 inhibits migration and invasion of fibroblast-like synoviocytes in rheumatoid arthritis.
Authors: Li D, Xiao Z, Wang G, Song X
Abstract
Rheumatoid arthritis (RA) is a systemic autoimmune disease with high rates of morbidity and mortality. Previous studies proposed that the A disintegrin and metalloprotease (ADAM) family is involved in the regulation of inflammation and arthritis. Thus, the present study investigated whether ADAM10 is involved in the progression of RA. The effects of ADAM10 small interfering (si)RNA on the expression levels of tumor necrosis factor (TNF)‑α, interleukin (IL)‑6, IL‑8 and chemokine (C-X-C motif) ligand 16 (CXCL16) were determined in fibroblast‑like synoviocytes (...
Source: Molecular Medicine Reports - July 3, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats.
Authors: Wang Y, Li Q, Wei X, Xu J, Chen Q, Song S, Lu Z, Wang Z
Abstract
Subacromial bursitis (SAB) is the major source of pain in rotator cuff disease. Although multiple investigations have provided support for the role of inflammatory cytokines in SAB, few have focussed on the use these cytokines in the treatment of SAB. The aim of the present study was to observe the therapeutic efficacy of lentivirus‑mediated RNA interference (RNAi) on carrageenan‑induced SAB by injecting lentivirus‑tumor necrosis factor (TNF)‑α‑RNAi expressing TNF‑α small interfering (si)RNA. Using screened siRNA segments, an si...
Source: Molecular Medicine Reports - July 3, 2015 Category: Molecular Biology Tags: Mol Med Rep Source Type: research
KPNA2 Contributes to the Inflammatory Processes in Synovial Tissue of Patients with Rheumatoid Arthritis and SW982 Cells
Abstract
Karyopherin-α2 (KPNA2) functions as an adaptor that transports several proteins to the nucleus. We investigated the function and possible mechanisms of KPNA2 involved in rheumatoid arthritis (RA). Western blotting and immunohistochemistry showed the protein expression of KPNA2 increased in synovial tissue of RA patients compared with the healthy controls. Double immunofluorescent staining indicated that KPNA2 co-localized with T cells, macrophage-like synoviocytes, fibroblast-like synoviocytes, and neutrophils in synovial tissue of RA patients. Moreover, the expression of KPNA2 in SW982 cells was increas...
Source: Inflammation - July 2, 2015 Category: Allergy & Immunology Source Type: research
Interleukin‐1 Acts via the JNK‐2 Signaling Pathway to Induce Aggrecan Degradation by Human Chondrocytes
ConclusionThe signaling axis TRAF‐6/TAK‐1/MKK‐4/JNK‐2 mediates IL‐1–induced aggrecanolysis. The level of aggrecanase is controlled by its endocytosis, which may be reduced upon IL‐1 stimulation because of LRP‐1 shedding.
Source: Arthritis and Rheumatism - June 26, 2015 Category: Rheumatology Authors: Heba M. Ismail, Kazuhiro Yamamoto, Tonia L. Vincent, Hideaki Nagase, Linda Troeberg, Jeremy Saklatvala Tags: Chondrocyte Biology Source Type: research
siRNA Delivery Impedes the Temporal Expression of Cytokine-Activated VCAM1 on Endothelial Cells.
Abstract
Leukocyte recruitment plays a key role in chronic inflammatory diseases such as cardiovascular disease, rheumatoid arthritis, and cancer. Leukocyte rolling and arrest are mediated in part by the temporally-regulated surface expression of vascular cell adhesion molecule-1 (VCAM1) on endothelial cells (ECs). In this paper, we engineered a pH-responsive vehicle comprised of 30 mol% dimethylaminoethyl methacrylate (30D) and 70 mol% hydroxyethyl methacrylate (70H) to encapsulate, protect, and deliver VCAM1 small interfering RNA (siRNA). The ability of siRNA to reduce VCAM1 gene expression is in direct opposi...
Source: Annals of Biomedical Engineering - June 23, 2015 Category: Biomedical Engineering Authors: Ho TT, You JO, Auguste DT Tags: Ann Biomed Eng Source Type: research
Expression of PADI4 in patients with ankylosing spondylitis and its role in mediating the effects of TNF-α on the proliferation and osteogenic differentiation of human mesenchymal stem cells.
In conclusion, the findings of this study demonstrate that the expression of PADI4 differs between patients with AS and normal subjects. In addition, our data suggest that PADI4 plays a role in hMSC proliferation and differentiation, which are induced by TNF-α.
PMID: 26082376 [PubMed - as supplied by publisher]
Source: International Journal of Molecular Medicine - June 16, 2015 Category: Molecular Biology Authors: Yang Y, Dai M Tags: Int J Mol Med Source Type: research
Anti-Citrullinated Protein Antibodies Induce Macrophage Subset Disequilibrium in RA Patients
Abstract
We used samples from rheumatoid arthritis (RA) patients to examine whether Anti-citrullinated protein antibodies (ACPAs) alter macrophage subset distribution and promote RA development. Macrophage subset distributions and interferon regulatory factor 4 (IRF4) and IRF5 expressions were analyzed. ACPAs were purified by affinity column. After RA and osteoarthritis (OA) patients’ macrophages were cocultured with ACPAs, macrophage subsets and IRF4 and IRF5 expressions were measured. Small interfering RNAs (siRNAs) were transfected into ACPA-activated cells to suppress IRF4 or IRF5. Fluorescence-activated cel...
Source: Inflammation - June 11, 2015 Category: Allergy & Immunology Source Type: research
Sonic hedgehog signalling pathway regulates apoptosis through Smo protein in human umbilical vein endothelial cells
Conclusion. The Shh signalling pathway plays a role in regulating endothelial cell apoptosis in a Smo-dependent manner.
Source: Rheumatology - May 19, 2015 Category: Rheumatology Authors: Zhu, S.-l., Luo, M.-q., Peng, W.-x., Li, Q.-x., Feng, Z.-y., Li, Z.-x., Wang, M.-x., Feng, X.-x., Liu, F., Huang, J.-l. Tags: Rheumatoid Arthritis BASIC SCIENCE Source Type: research
Evidence for cadherin-11 cleavage in the synovium and partial characterization of its mechanism
Conclusions:
Cadherin-11 undergoes ectodomain shedding followed by regulated intramembrane proteolysis in synovial fibroblasts, triggered by a novel sheddase that generates extracelluar cadherin-11 fragments. Cadherin-11 fragments were enriched in RA synovial fluid, suggesting they may be a marker of synovial burden and may function to modify cadherin-11 interactions between synovial fibroblasts.
Source: Arthritis Research and Therapy - May 15, 2015 Category: Rheumatology Authors: Erika NossGerald WattsDavide ZoccoTracy KellerMalcolm WhitmanCarl BlobelDavid LeeMichael Brenner Source Type: research
Hypoxia and STAT3 signalling interactions regulate pro-inflammatory pathways in rheumatoid arthritis
Conclusions
This is the first study to provide evidence of a functional link between HIF1α, STAT3 and Notch-1 signalling in the regulation of pro-inflammatory mechanisms in RA, and further supports a role for STAT blockade in the treatment of RA.
Source: Annals of the Rheumatic Diseases - May 8, 2015 Category: Rheumatology Authors: Gao, W., McCormick, J., Connolly, M., Balogh, E., Veale, D. J., Fearon, U. Tags: Immunology (including allergy), Connective tissue disease, Degenerative joint disease, Musculoskeletal syndromes, Rheumatoid arthritis Basic and translational research Source Type: research
A disintegrin and metalloprotease-10 is correlated with disease activity and mediates monocyte migration and adhesion in rheumatoid arthritis
We examined the secretion of ADAM-10 in biological fluids from patients with rheumatoid arthritis (RA) and the role it plays in monocyte migration. ADAM-10 levels were measured using enzyme-linked immunosorbent assays and immunofluorescence. To examine the role of ADAM-10 in RA synovial fluids, we studied THP-1 and monocyte chemotaxis. To determine whether ADAM-10 plays a role in cell proliferation in the RA synovium, we assayed the proliferation of ADAM-10 siRNA-transfected RA fibroblast-like synoviocytes (FLS).
Source: Translational Research - February 28, 2015 Category: Research Authors: Takeo Isozaki, Sho Ishii, Shinichiro Nishimi, Airi Nishimi, Nao Oguro, Shinya Seki, Yoko Miura, Yusuke Miwa, Koei Oh, Yoichiro Toyoshima, Masanori Nakamura, Katsunori Inagaki, Tsuyoshi Kasama Source Type: research
