Dihydroartemisinin attenuates lipopolysaccharide ‑induced acute lung injury in mice by suppressing NF‑κB signaling in an Nrf2‑dependent manner.
Dihydroartemisinin attenuates lipopolysaccharide‑induced acute lung injury in mice by suppressing NF‑κB signaling in an Nrf2‑dependent manner. Int J Mol Med. 2019 Oct 29;: Authors: Huang XT, Liu W, Zhou Y, Hao CX, Zhou Y, Zhang CY, Sun CC, Luo ZQ, Tang SY Abstract Acute lung injury (ALI) is a severe health issue with significant morbidity and mortality. Artemisinin is used for the treatment of fever and malaria in clinical practice. Dihydroartemisinin (DHA), the major active metabolite of artemisinin, plays a role in anti‑organizational fibrosis and anti‑neuronal cell death. However, w...
Source: International Journal of Molecular Medicine - October 29, 2019 Category: Molecular Biology Authors: Huang XT, Liu W, Zhou Y, Hao CX, Zhou Y, Zhang CY, Sun CC, Luo ZQ, Tang SY Tags: Int J Mol Med Source Type: research

RFC3 induces epithelial ‑mesenchymal transition in lung adenocarcinoma cells through the Wnt/β‑catenin pathway and possesses prognostic value in lung adenocarcinoma.
In conclusion, RFC3 may be considered a coactivator that promotes the Wnt/β‑catenin signaling pathway, and induces EMT and metastasis in lung adenocarcinoma. PMID: 31661124 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 29, 2019 Category: Molecular Biology Authors: Gong S, Qu X, Yang S, Zhou S, Li P, Zhang Q Tags: Int J Mol Med Source Type: research

Glutamine affects T24 bladder cancer cell proliferation by activating STAT3 through ROS and glutaminolysis.
In this study, we aimed to to explore the mechanisms through which bladder cancer cells utilize Gln to fulfill their biosynthetic needs during proliferation. In addition, the roles of Gln in the tricarboxylic acid (TCA) cycle, reactive oxygen species (ROS) regulation, and signal transducer and activator of transcription 3 (STAT3) expression were examined in vitro in the T24 bladder cancer cell line. The results revealed that the T24 cell line was markedly Gln‑dependent and that Gln supplementation promoted T24 proliferation through the actions of Gln as a ROS moderator and as a metabolic fuel in ...
Source: International Journal of Molecular Medicine - October 25, 2019 Category: Molecular Biology Authors: Sun N, Liang Y, Chen Y, Wang L, Li D, Liang Z, Sun L, Wang Y, Niu H Tags: Int J Mol Med Source Type: research

Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in  vitro.
Rutin promotes the formation and osteogenic differentiation of human periodontal ligament stem cell sheets in vitro. Int J Mol Med. 2019 Oct 25;: Authors: Zhao B, Zhang Y, Xiong Y, Xu X Abstract Cell sheet technology is a novel tissue engineering technology that has been rapidly developed in recent years. As a novel technology, cell sheet technology is expected to become one of the preferred methods for cell transplantation. The present study investigated the biological effects of rutin on the formation of periodontal ligament stem cell (PDLSC) sheets and their resultant osteogenic properties. Th...
Source: International Journal of Molecular Medicine - October 25, 2019 Category: Molecular Biology Authors: Zhao B, Zhang Y, Xiong Y, Xu X Tags: Int J Mol Med Source Type: research

The goat β‑casein/CMV chimeric promoter drives the expression of hLF in transgenic goats produced by cell transgene microinjection.
The objective of this study was to produce transgenic cloned goats that would serve as living bioreactors, expressing high levels of recombinant human LF (rhLF) in their milk. We designed a pCL25 expression vector containing goat β‑casein/CMV chimeric promoter in order to facilitate rhLF expression. This pCL25‑rhLF‑Neo vector was microinjected into goat fetal fibroblasts. G418 selection and PCR analysis were used to identify transgenic donor cells suitable for somatic cell nuclear transfer (SCNT). After SCNT and embryo transplantation, goats harboring the hLF gene were produced, as confirmed via PCR and southern...
Source: International Journal of Molecular Medicine - October 24, 2019 Category: Molecular Biology Authors: Zhang T, Yuan Y, Lu R, Xu S, Zhou M, Yuan T, Lu Y, Yan K, Cheng Y Tags: Int J Mol Med Source Type: research

Roles of p38 α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells.
Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells. Int J Mol Med. 2019 Oct 24;: Authors: Wen SY, Cheng SY, Ng SC, Aneja R, Chen CJ, Huang CY, Kuo WW Abstract Skin cancer is one of the most common cancers worldwide. Melanoma accounts for ~5% of skin cancers but causes the large majority of skin cancer‑related deaths. Recent discoveries have shown that the mitogen‑activated protein kinase (MAPK) signaling pathway is critical for melanoma development and progression. Many oncogenic pathways that cause melanoma tumorigenesis have been ...
Source: International Journal of Molecular Medicine - October 24, 2019 Category: Molecular Biology Authors: Wen SY, Cheng SY, Ng SC, Aneja R, Chen CJ, Huang CY, Kuo WW Tags: Int J Mol Med Source Type: research

Kaempferol ‑3‑O‑β‑rutinoside suppresses the inflammatory responses in lipopolysaccharide‑stimulated RAW264.7 cells via the NF‑κB and MAPK pathways.
Kaempferol‑3‑O‑β‑rutinoside suppresses the inflammatory responses in lipopolysaccharide‑stimulated RAW264.7 cells via the NF‑κB and MAPK pathways. Int J Mol Med. 2019 Oct 22;: Authors: Hwang D, Kang MJ, Kang CW, Kim GD Abstract Kaempferol‑3‑O‑β‑rutinoside is one of the compounds isolated from tartary buckwheat (Fagopyrum tatricum), and its biological effects have not been studied yet. The present study examined the anti‑inflammatory effects of kaempferol‑3‑O‑β‑rutinoside and explore its regulatory mechanisms in lipopolysaccharide (LPS)‑induced macro...
Source: International Journal of Molecular Medicine - October 22, 2019 Category: Molecular Biology Authors: Hwang D, Kang MJ, Kang CW, Kim GD Tags: Int J Mol Med Source Type: research

miR ‑146a‑5p suppresses ATP‑binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor‑associated kinase 1 downregulation.
miR‑146a‑5p suppresses ATP‑binding cassette subfamily G member 1 dysregulation in patients with refractory Mycoplasma pneumoniae via interleukin 1 receptor‑associated kinase 1 downregulation. Int J Mol Med. 2019 Oct 22;: Authors: Li HN, Zhao X, Zha YJ, Du F, Liu J, Sun L Abstract In the present study, we examined the function of microRNA (miR)‑146a‑5p in patients with refractory Mycoplasma pneumoniae pneumonia. In brief, the expression of miR‑146a‑5p was reduced in patients with refractory Mycoplasma pneumoniae pneumonia. Downregulation of miR‑146a‑5p reduced inflammation in a...
Source: International Journal of Molecular Medicine - October 22, 2019 Category: Molecular Biology Authors: Li HN, Zhao X, Zha YJ, Du F, Liu J, Sun L Tags: Int J Mol Med Source Type: research

Leiomyoma phosphoproteins involved in inhibition of oxidative stress and synthesis of reactive oxygen species.
Abstract Uterine leiomyomas are benign smooth muscle cell tumors originating from the myometrium. The present study focused on leiomyoma and myometrium phosphoproteome enrichment by using immobilized metal affinity chromatography (IMAC). The phosphoproteome was analyzed by two‑dimensional gel electrophoresis coupled with mass spectrometry. Western blotting was used for data validation. The results from IMAC identified 26 proteins significantly differentially phosphorylated in leiomyomas compared with normal myometrium. Three upregulated proteins (peroxiredoxin 2, protein disulfide isomerase family A member 3 and...
Source: International Journal of Molecular Medicine - October 21, 2019 Category: Molecular Biology Authors: Ura B, Monasta L, Arrigoni G, Licastro D, Di Lorenzo G, Romano F, Gaita B, Scrimin F, Ricci G Tags: Int J Mol Med Source Type: research

TROP2 increases growth and metastasis of human oral squamous cell carcinoma through activation of the PI3K/Akt signaling pathway.
Abstract Most malignant neoplasms of the oral cavity are oral squamous cell carcinoma (OSCC), which is a type of highly malignant tumor with a propensity for forming distant metastases. Trophoblast cell surface antigen 2 (TROP2) is a transmembrane protein that is overexpressed in several types of tumor cells, although its role and regulatory mechanism in OSCC have not been determined. The aim of the present study was to examine the effects of TROP2 in human OSCC cell lines. The present study demonstrated that TROP2 protein expression was upregulated in OSCC cell lines. Transfection of short hairpin RNA (shRNA) tar...
Source: International Journal of Molecular Medicine - October 21, 2019 Category: Molecular Biology Authors: Tang G, Tang Q, Jia L, Chen Y, Lin L, Kuai X, Gong A, Feng Z Tags: Int J Mol Med Source Type: research

Upregulation of sphingosine kinase  1 contributes to ventilator-associated lung injury in a two-hit model.
Upregulation of sphingosine kinase 1 contributes to ventilator-associated lung injury in a two-hit model. Int J Mol Med. 2019 Oct 21;: Authors: Wang Y, Gao TT, Xu DF, Zhu XY, Dong WW, Lv Z, Liu YJ, Jiang L Abstract Ventilator‑associated lung injury (VALI) remains a significant medical problem in intensive care units. The present study aimed to investigate the role of sphingosine kinase 1 (SPHK1) in VALI using a two‑hit model and explore the potential underlying molecular mechanism. Mice were divided into five groups: i) Non‑ventilated group; ii) non‑ventilated + lipopo...
Source: International Journal of Molecular Medicine - October 21, 2019 Category: Molecular Biology Authors: Wang Y, Gao TT, Xu DF, Zhu XY, Dong WW, Lv Z, Liu YJ, Jiang L Tags: Int J Mol Med Source Type: research

Role and mechanisms of action of microRNA ‑21 as regards the regulation of the WNT/β‑catenin signaling pathway in the pathogenesis of non‑alcoholic fatty liver disease.
Role and mechanisms of action of microRNA‑21 as regards the regulation of the WNT/β‑catenin signaling pathway in the pathogenesis of non‑alcoholic fatty liver disease. Int J Mol Med. 2019 Oct 18;: Authors: Wang XM, Wang XY, Huang YM, Chen X, Lü MH, Shi L, Li CP Abstract The aim of the present study was to investigate the role of microRNA‑21 (miR‑21) in regulating the classical WNT/β‑catenin signaling pathway by targeting low‑density lipoprotein‑related receptor 6 (LRP6) in non‑alcoholic fatty liver disease (NAFLD). For this purpose, we established a NAFLD model by...
Source: International Journal of Molecular Medicine - October 18, 2019 Category: Molecular Biology Authors: Wang XM, Wang XY, Huang YM, Chen X, Lü MH, Shi L, Li CP Tags: Int J Mol Med Source Type: research

Osteoadherin serves roles in the regulation of apoptosis and growth in MC3T3 ‑E1 osteoblast cells.
Osteoadherin serves roles in the regulation of apoptosis and growth in MC3T3‑E1 osteoblast cells. Int J Mol Med. 2019 Oct 18;: Authors: Hamaya E, Fujisawa T, Tamura M Abstract Small leucine‑rich proteoglycans (SLRPs) are a class of proteoglycans that are characterized by small protein cores and structures of leucine‑rich repeats. SLRPs are expressed in most extracellular matrices and share numerous biological functions that are associated with binding of collagens and cell surface receptors. Osteoadherin (also termed osteomodulin) is encoded by the Omd gene and is a keratan sulfate proteoglycan ...
Source: International Journal of Molecular Medicine - October 18, 2019 Category: Molecular Biology Authors: Hamaya E, Fujisawa T, Tamura M Tags: Int J Mol Med Source Type: research

Adipose ‑derived stem cells overexpressing SK4 calcium‑activated potassium channel generate biological pacemakers.
In conclusion, SK4 induced ADSCs to differentiate into cardiomyocyte‑like and pacemaker‑like cells via activation of the extracellular signal‑regulated kinase 1/2 and p38 mitogen‑activated protein kinase pathways. Therefore, ADSCs transduced with SK4 may be used to generate biological pacemakers in ex vivo rat hearts. PMID: 31638180 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 16, 2019 Category: Molecular Biology Authors: Yang M, Zhao Q, Zhao H, Yang A, Wang F, Wang X, Tang Y, Huang C Tags: Int J Mol Med Source Type: research

Bioinformatics analysis of Ras homologue enriched in the striatum, a potential target for Huntington's disease therapy.
Abstract Huntington's disease (HD) is a lethal neurodegenerative disorder for which no cure is available yet. It is caused by abnormal expansion of a CAG triplet in the gene encoding the huntingtin protein (Htt), with consequent expansion of a polyglutamine repeat in mutated Htt (mHtt). This makes mHtt highly unstable and aggregation prone. Soluble mHtt is linked to cytotoxicity and neurotoxicity, whereas mHtt aggregates are thought to be neuroprotective. While Htt and mHtt are ubiquitously expressed throughout the brain and peripheral tissues, HD is characterized by selective degradation of the corpus striatum, w...
Source: International Journal of Molecular Medicine - October 15, 2019 Category: Molecular Biology Authors: Carbo M, Brandi V, Pascarella G, Staid DS, Colotti G, Polticelli F, Ilari A, Morea V Tags: Int J Mol Med Source Type: research

TPX2 silencing exerts anti ‑tumor effects on hepatocellular carcinoma by regulating the PI3K/AKT signaling pathway.
In conclusion, TPX2 may suppress the growth of HCC by regulating the PI3K/AKT signaling pathway and thus, TPX2 may be a potential target for the treatment of liver cancer. PMID: 31638175 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 14, 2019 Category: Molecular Biology Authors: Huang DH, Jian J, Li S, Zhang Y, Liu LZ Tags: Int J Mol Med Source Type: research

Feasibility of tumor ‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia.
Feasibility of tumor‑derived exosome enrichment in the onco‑hematology leukemic model of chronic myeloid leukemia. Int J Mol Med. 2019 Oct 14;: Authors: Bernardi S, Foroni C, Zanaglio C, Re F, Polverelli N, Turra A, Morello E, Farina M, Cattina F, Gandolfi L, Zollner T, Buttini EA, Malagola M, Russo D Abstract Due to the discovery of their role in intra‑cellular communications, exosomes, which carry information specific to the cell of origin, have garnered considerable attention in cancer research. Moreover, there is evidence to suggest the possibility of isolating different exosome sub‑popula...
Source: International Journal of Molecular Medicine - October 14, 2019 Category: Molecular Biology Authors: Bernardi S, Foroni C, Zanaglio C, Re F, Polverelli N, Turra A, Morello E, Farina M, Cattina F, Gandolfi L, Zollner T, Buttini EA, Malagola M, Russo D Tags: Int J Mol Med Source Type: research

lncRNA/mRNA profiling of endometriosis rat uterine tissues during the implantation window.
Abstract Endometriosis is associated with changes in long non‑coding RNA (lncRNA) and mRNA expression, but the exact changes during the implantation window are unknown. Therefore, this study aimed to explore the lncRNA and mRNA expression profiles in the uterus of rats with endometriosis during the implantation window. A total of 35 non‑pregnant female rats were randomized to the endometriosis (n=13), adipose tissue control (n=8) and blank control (n=14) groups. On the 5th day of pregnancy, the rats were sacrificed to obtain uterine tissues. lncRNA and mRNA were analyzed using gene chips. A total of five diffe...
Source: International Journal of Molecular Medicine - October 14, 2019 Category: Molecular Biology Authors: Cai H, Zhu X, Li Z, Zhu Y, Lang J Tags: Int J Mol Med Source Type: research

Influence of radiation exposure pattern on the bone injury and osteoclastogenesis in a rat model.
Abstract Radiotherapy, one of the clinical treatments of cancer, is accompanied by a high risk of damage to healthy tissues, such as bone loss and increased risk of fractures. The aim of the present study was to establish a rat model of local and systemic bone injury by focal irradiation, in order to study the etiological mechanism and intervention. The proximal metaphyseal region of the left hindlimb of male Sprague‑Dawley rats were exposed to a single 2 Gy or three 8 Gy doses delivered on days 1, 3 and 5 using a small animal irradiator, the changes in bone volume and microarchitecture were evaluated,...
Source: International Journal of Molecular Medicine - October 11, 2019 Category: Molecular Biology Authors: Zhai J, He F, Wang J, Chen J, Tong L, Zhu G Tags: Int J Mol Med Source Type: research

Tacrolimus increases the expression level of the chemokine receptor CXCR2 to promote renal fibrosis progression.
Abstract Tacrolimus is one of the most used and effective immunosuppressive agents currently available in the clinic; however, its use is limited by nephrotoxicity, which is the main secondary effect of this drug. The mechanisms underlying tacrolimus‑induced nephrotoxicity remain unknown. The present study aimed to investigate the mechanism underlying tacrolimus‑induced nephrotoxicity and to identify novel potential targets. Masson staining, Sirius red staining and periodic acid‑silver methenamine staining were used to observe kidney pathological changes. Immunohistochemical and immunofluorescent analyses we...
Source: International Journal of Molecular Medicine - October 10, 2019 Category: Molecular Biology Authors: Wang D, Chen X, Fu M, Xu H, Li Z Tags: Int J Mol Med Source Type: research

Pistacia weinmannifolia root exerts a protective role in ovalbumin ‑induced lung inflammation in a mouse allergic asthma model.
Pistacia weinmannifolia root exerts a protective role in ovalbumin‑induced lung inflammation in a mouse allergic asthma model. Int J Mol Med. 2019 Oct 07;: Authors: Lee JW, Min JH, Kim MG, Kim SM, Kwon OK, Oh TK, Lee JK, Kim TY, Lee SW, Choi S, Li WY, Ryu HW, Ahn KS, Oh SR Abstract Pistacia weinmannifolia (Anacardiaceae) has been used in herbal medicine for the treatment of influenza, dysentery and enteritis in China. It was recently observed that P. weinmannifolia root extract (PWRE) exerts anti‑inflammatory effects both in in vitro and in vivo models. Based on the results from previous studies, ...
Source: International Journal of Molecular Medicine - October 7, 2019 Category: Molecular Biology Authors: Lee JW, Min JH, Kim MG, Kim SM, Kwon OK, Oh TK, Lee JK, Kim TY, Lee SW, Choi S, Li WY, Ryu HW, Ahn KS, Oh SR Tags: Int J Mol Med Source Type: research

Redd1 protects against post ‑infarction cardiac dysfunction by targeting apoptosis and autophagy.
In conclusion, this study demonstrated that Redd1 overexpression protects against the development and persistence of heart failure post MI by reducing apoptosis and enhancing autophagy via the mTOR signaling pathway. The present study clearly demonstrated that Redd1 is a therapeutic target in the development of heart failure after MI. PMID: 31638187 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 4, 2019 Category: Molecular Biology Authors: Huang P, Fu J, Chen L, Ju C, Wu K, Liu H, Liu Y, Qi B, Qi B, Liu L Tags: Int J Mol Med Source Type: research

The A3 adenosine receptor agonist, namodenoson, ameliorates non ‑alcoholic steatohepatitis in mice.
The A3 adenosine receptor agonist, namodenoson, ameliorates non‑alcoholic steatohepatitis in mice. Int J Mol Med. 2019 Oct 03;: Authors: Fishman P, Cohen S, Itzhak I, Amer J, Salhab A, Barer F, Safadi R Abstract The Wnt/β‑catenin pathway confers a chain of molecular events in livers affected by non‑alcoholic steatohepatitis (NASH). Namodenoson, a selective agonist of the A3 adenosine receptor (A3AR), which is highly expressed in pathological liver cells, induces a robust anti‑inflammatory effect in the liver, mediated via the de‑regulation of the Wnt/β‑catenin path...
Source: International Journal of Molecular Medicine - October 3, 2019 Category: Molecular Biology Authors: Fishman P, Cohen S, Itzhak I, Amer J, Salhab A, Barer F, Safadi R Tags: Int J Mol Med Source Type: research

Genistein improves viability, proliferation and mitochondrial function of cardiomyoblasts cultured in physiologic and peroxidative conditions.
Abstract Phytoestrogens exert protective effects on the cardiovascular system through mechanisms that have yet to be clearly demonstrated. The aim of this study was to evaluate the protective effects exerted by genistein on cardiomyoblasts (H9C2) against oxidative stress, nitric oxide (NO) release, viability, proliferation/migration and mitochondrial function. H9C2 cultured in physiological or peroxidative conditions, were treated with genistein in the absence or presence of estrogen receptors (ERs), G protein‑​coupled‑estrogenic‑receptors (GPER), Akt, extracellular‑​signal‑regulated kinases 1/2 (ERK...
Source: International Journal of Molecular Medicine - October 3, 2019 Category: Molecular Biology Authors: Farruggio S, Raina G, Cocomazzi G, Librasi C, Mary D, Gentilli S, Grossini E Tags: Int J Mol Med Source Type: research

Apoptosis induced by bruceine  D in human non‑small‑cell lung cancer cells involves mitochondrial ROS‑mediated death signaling.
Apoptosis induced by bruceine D in human non‑small‑cell lung cancer cells involves mitochondrial ROS‑mediated death signaling. Int J Mol Med. 2019 Oct 03;: Authors: Xie JH, Lai ZQ, Zheng XH, Xian YF, Li Q, Ip SP, Xie YL, Chen JN, Su ZR, Lin ZX, Yang XB Abstract Bruceine D is one of the active components of Brucea javanica (L.) Merr., which is widely used to treat cancer in China. The aim of the present study was to evaluate the potential effect of bruceine D against non‑small‑cell lung cancer (NSCLC) cells and delineate its underlying mechanisms. The results indicated that tre...
Source: International Journal of Molecular Medicine - October 3, 2019 Category: Molecular Biology Authors: Xie JH, Lai ZQ, Zheng XH, Xian YF, Li Q, Ip SP, Xie YL, Chen JN, Su ZR, Lin ZX, Yang XB Tags: Int J Mol Med Source Type: research

Tumor cell ‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer.
Tumor cell‑fibroblast heterotypic aggregates in malignant ascites of patients with ovarian cancer. Int J Mol Med. 2019 Oct 02;: Authors: Han Q, Huang B, Huang Z, Cai J, Gong L, Zhang Y, Jiang J, Dong W, Wang Z Abstract Ascitic multicellular aggregates (MCAs) promote peritoneal metastasis of ovarian cancer. The aim of the present study was to elucidate the role of cancer‑associated fibroblasts (CAFs) in MCA formation and metastasis in patients with high‑grade serous ovarian cancer (HGSOC). Immunohistochemistry was used to identify the cell phenotypes and the presence of CAFs in ascitic MCAs....
Source: International Journal of Molecular Medicine - October 2, 2019 Category: Molecular Biology Authors: Han Q, Huang B, Huang Z, Cai J, Gong L, Zhang Y, Jiang J, Dong W, Wang Z Tags: Int J Mol Med Source Type: research

Screening for differentially ‑expressed microRNA biomarkers in Saudi colorectal cancer patients by small RNA deep sequencing.
Screening for differentially‑expressed microRNA biomarkers in Saudi colorectal cancer patients by small RNA deep sequencing. Int J Mol Med. 2019 Oct 02;: Authors: Al-Sheikh YA, Ghneim HK, Alharbi KK, Aboul-Soud MAM Abstract Colorectal cancer (CRC) is mostly diagnosed at late stages leading to high mortality rates due to the scarcity of efficient screening approaches exhibiting high diagnostic utility. The current study employed a small‑RNA deep‑sequencing approach for screening microRNA (miRNA) differentially expressed genes (DEGs), and evaluating their potential as early dia...
Source: International Journal of Molecular Medicine - October 2, 2019 Category: Molecular Biology Authors: Al-Sheikh YA, Ghneim HK, Alharbi KK, Aboul-Soud MAM Tags: Int J Mol Med Source Type: research

Stop codons and the +4 nucleotide may influence the efficiency of G418 in rescuing nonsense mutations of the HERG gene.
Abstract The importance of the local sequence context in determining how efficiently aminoglycosides rescue nonsense mutations has been established previously in disease models. Different stop codons appear to facilitate the termination process with differing efficiencies. Furthermore, the efficiency with which termination is suppressed may also be influenced by the local sequence context surrounding the stop codon. The strongest bias has usually been identified with the nucleotide base that immediately follows the stop codon in the majority of experiments. However, how the sequence context influences the efficien...
Source: International Journal of Molecular Medicine - October 1, 2019 Category: Molecular Biology Authors: Yu H, Meng Y, Zhang S, Tian C, Wu F, Li N, Li Q, Jin Y, Pu J Tags: Int J Mol Med Source Type: research

Protective effect of hydrogen sulfide on monocrotaline ‑induced pulmonary arterial hypertension via inhibition of the endothelial mesenchymal transition.
In conclusion, the current study provides novel information elucidating the beneficial effect of H2S on PAH through inhibiting the induction of the NF‑κB‑Snail pathway and the subsequent process of EndMT in pulmonary arteries. PMID: 31573044 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 1, 2019 Category: Molecular Biology Authors: Zhang H, Lin Y, Ma Y, Zhang J, Wang C, Zhang H Tags: Int J Mol Med Source Type: research

Resveratrol attenuates pulmonary embolism associated cardiac injury by suppressing activation of the inflammasome via the MALAT1 ‑miR‑22‑3p signaling pathway.
In conclusion, it was demonstrated that RS has been demonstrated to prevent the development of cardiac injury induced by PE via modulating the expression of MALAT1 and further affect miR‑22‑3p and NLRP3. PMID: 31573048 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - October 1, 2019 Category: Molecular Biology Authors: Yang K, Li W, Duan W, Jiang Y, Huang N, Li Y, Ren B, Sun J Tags: Int J Mol Med Source Type: research

miR ‑19a promotes vascular smooth muscle cell proliferation, migration and invasion through regulation of Ras homolog family member B.
miR‑19a promotes vascular smooth muscle cell proliferation, migration and invasion through regulation of Ras homolog family member B. Int J Mol Med. 2019 Sep 27;: Authors: Sun G, Song H, Wu S Abstract Diabetic patients with high glucose exhibit vascular smooth muscle cell (VSMC) alteration. Thrombotic disease is related to erosion of an unstable plaque, the instability of which leads to ruptures, for example, a thin fibrous cap derived from VSMCs. VSMC proliferation, migration and invasion are related to thrombotic diseases, including atherosclerosis. MicroRNA‑19a (miR‑19a) has been reported to ...
Source: International Journal of Molecular Medicine - September 27, 2019 Category: Molecular Biology Authors: Sun G, Song H, Wu S Tags: Int J Mol Med Source Type: research

FSH1 regulates the phenotype and pathogenicity of the pathogenic dermatophyte Microsporum canis.
Abstract Microsporum canis (M. canis) is a common pathogen that causes tinea capitis and is present worldwide. The incidence of M. canis infection, particularly tinea capitis, has been increasing in China. In our previous studies, family of serine hydrolases 1 (FSH1) was identified as a potential virulence factor in tinea capitis infection caused by M. canis. To determine the function of this gene in M. canis, FSH1 was knocked down using double‑stranded RNA interference mediated by Agrobacterium tumefaciens. Reverse transcription‑quantitative PCR analysis was used to confirm gene knockdown. Loss of FSH1 expres...
Source: International Journal of Molecular Medicine - September 27, 2019 Category: Molecular Biology Authors: Zhang F, Tan C, Xu Y, Yang G Tags: Int J Mol Med Source Type: research

Biological effects of melatonin on human adipose ‑derived mesenchymal stem cells.
Biological effects of melatonin on human adipose‑derived mesenchymal stem cells. Int J Mol Med. 2019 Sep 27;: Authors: Heo JS, Pyo S, Lim JY, Yoon DW, Kim BY, Kim JH, Kim GJ, Lee SG, Kim J Abstract Mesenchymal stem cells (MSCs) are capable of differentiating into other cell types and exhibit immunomodulatory effects. MSCs are affected by several intrinsic and extrinsic signaling modulators, including growth factors, cytokines, extracellular matrix and hormones. Melatonin, produced by the pineal gland, is a hormone that regulates sleep cycles. Recent studies have shown that melatonin improves the the...
Source: International Journal of Molecular Medicine - September 27, 2019 Category: Molecular Biology Authors: Heo JS, Pyo S, Lim JY, Yoon DW, Kim BY, Kim JH, Kim GJ, Lee SG, Kim J Tags: Int J Mol Med Source Type: research

Improved HCN channels in pyramidal neurons and their new expression levels in pericytes and astrocytes in the gerbil hippocampal CA1 subfield following transient ischemia.
Abstract Hyperpolarization‑activated cyclic nucleotide‑gated (HCN) channels have been known to participate in the regulation of neuronal excitability, synaptic transmission and long‑term potentiation in the hippocampus. The present study investigated transient ischemia‑induced changes of HCN1 and HCN2 expressions in the Cornu Ammonis 1 (CA1) subfield of the hippocampus in gerbils subjected to 5 min transient global cerebral ischemia (tgCI). Neuronal death was exhibited in pyramidal neurons of the striatum pyramidale in the CA1 subfield 4 days after tgCI. HCN1 and HCN2 immunoreactivities were demonstrated i...
Source: International Journal of Molecular Medicine - September 26, 2019 Category: Molecular Biology Authors: Park JH, Kim DW, Lee TK, Park CW, Park YE, Ahn JH, Lee HA, Won MH, Lee CH Tags: Int J Mol Med Source Type: research

S ‑allyl‑cysteine sulfoxide (alliin) alleviates myocardial infarction by modulating cardiomyocyte necroptosis and autophagy.
S‑allyl‑cysteine sulfoxide (alliin) alleviates myocardial infarction by modulating cardiomyocyte necroptosis and autophagy. Int J Mol Med. 2019 Sep 26;: Authors: Yue LJ, Zhu XY, Li RS, Chang HJ, Gong B, Tian CC, Liu C, Xue YX, Zhou Q, Xu TS, Wang DJ Abstract S‑allyl‑cysteine sulfoxide (alliin) is the main organosulfur component of garlic and its preparations. The present study aimed to examine the protective effect of alliin on cardiac function and the underlying mechanism in a mouse model of myocardial infarction (MI). Notably, alliin treatment preserved heart function, attenuated the area of...
Source: International Journal of Molecular Medicine - September 26, 2019 Category: Molecular Biology Authors: Yue LJ, Zhu XY, Li RS, Chang HJ, Gong B, Tian CC, Liu C, Xue YX, Zhou Q, Xu TS, Wang DJ Tags: Int J Mol Med Source Type: research

The prospect of discovering new biomarkers for ovarian cancer based on current knowledge of  susceptibility loci and genetic variation (Review).
The prospect of discovering new biomarkers for ovarian cancer based on current knowledge of susceptibility loci and genetic variation (Review). Int J Mol Med. 2019 Sep 26;: Authors: Christophersen MK, Høgdall C, Høgdall E Abstract Ovarian cancer is the most lethal gynaecological malignancy. The cancer initially presents with non‑specific symptoms; thus, it is typically not discovered until the patient has reached the late, considerably more lethal, stages of the disease. Research focus is currently on finding novel biomarkers, especially for early detection and stratification of t...
Source: International Journal of Molecular Medicine - September 26, 2019 Category: Molecular Biology Authors: Christophersen MK, Høgdall C, Høgdall E Tags: Int J Mol Med Source Type: research

IL ‑21/IL‑21R inhibit tumor growth and invasion in non‑small cell lung cancer cells via suppressing Wnt/β‑catenin signaling and PD‑L1 expression.
In conclusion, the IL‑21/IL‑21R axis reduced the growth and invasion of NSCLC cells via inhibiting Wnt/β‑catenin signaling and PD‑L1 expression. The present results may provide a novel molecular target for NSCLC diagnosis and therapy. PMID: 31573051 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - September 26, 2019 Category: Molecular Biology Authors: Xue D, Yang P, Wei Q, Li X, Lin L, Lin T Tags: Int J Mol Med Source Type: research

Fatty liver and alteration of the gut microbiome induced by diallyl disulfide.
Abstract Diallyl disulfide (DADS) is one of the primary components of garlic and it exhibits a broad range of biological activities. In the present study, the effects of DADS on lipid metabolism and its potential role in the modulation of the gut microbiome were determined. Hematoxylin and eosin and oil‑red O staining were used to assess the liver and intestinal tissues of mice treated with DADS. The expression of lipid metabolism‑associated genes was measured using reverse transcription‑quantitative PCR (RT‑qPCR). The effects of DADS on the gut microbiome were measured using 16S recombinant (r)DNA gene an...
Source: International Journal of Molecular Medicine - September 24, 2019 Category: Molecular Biology Authors: Yang Y, Yang F, Huang M, Wu H, Yang C, Zhang X, Yang L, Chen G, Li S, Wang Q, Liu S, Liu Y, Lei Y, Lei Z, Guo J Tags: Int J Mol Med Source Type: research

Overexpressed CD39 mitigates sepsis ‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3.
Overexpressed CD39 mitigates sepsis‑induced kidney epithelial cell injury via suppressing the activation of NLR family pyrin domain containing 3. Int J Mol Med. 2019 Sep 23;: Authors: Yang M, Lu L, Kang Z, Ma T, Wang Y Abstract Unfettered inflammation is a leading cause of multiple organ failures in sepsis. The anti‑inflammatory role of cluster of differentiation (CD)39 has been previously reported. The present study aimed to investigate the role of unfettered inflammation in sepsis‑induced acute kidney injury (AKI). Lipopolysaccharide (LPS) was introduced to construct a sepsis mouse model. Kidn...
Source: International Journal of Molecular Medicine - September 23, 2019 Category: Molecular Biology Authors: Yang M, Lu L, Kang Z, Ma T, Wang Y Tags: Int J Mol Med Source Type: research

CUDC ‑907 reverses pathological phenotype of keloid fibroblasts in vitro and in vivo via dual inhibition of PI3K/Akt/mTOR signaling and HDAC2.
CUDC‑907 reverses pathological phenotype of keloid fibroblasts in vitro and in vivo via dual inhibition of PI3K/Akt/mTOR signaling and HDAC2. Int J Mol Med. 2019 Sep 23;: Authors: Tu T, Huang J, Lin M, Gao Z, Wu X, Zhang W, Zhou G, Wang W, Liu W Abstract Keloids are benign skin tumors with a high recurrence rate following surgical excision. Abnormal intracellular signaling is one of the key mechanisms involved in its pathogenesis. Over‑activated phosphoinositide 3‑kinase/RAC‑alpha serine/threonine‑protein kinase/mammalian target of rapamycin (PI3K/Akt/mTOR) signaling pathway and overproducti...
Source: International Journal of Molecular Medicine - September 23, 2019 Category: Molecular Biology Authors: Tu T, Huang J, Lin M, Gao Z, Wu X, Zhang W, Zhou G, Wang W, Liu W Tags: Int J Mol Med Source Type: research

Metabolomic profiling identifies novel biomarkers and mechanisms in human bladder cancer treated with submucosal injection of gemcitabine.
Abstract Bladder cancer (BCa) is a common urinary tract malignancy with frequent recurrences after initial resection. Submucosal injection of gemcitabine prior to transurethral resection of bladder tumor (TURBT) may prevent recurrence of urothelial cancer. However, the underlying mechanism remains unknown. In the present study, ultra‑performance liquid chromatography Q‑Exactive mass spectrometry was used to profile tissue metabolites from 12 BCa patients. The 48 samples included pre‑ and post‑gemcitabine treatment BCa tissues, as well as adjacent normal tissues. Principal component analysis (PCA) revealed ...
Source: International Journal of Molecular Medicine - September 23, 2019 Category: Molecular Biology Authors: Yang C, Sun X, Wang H, Lu T, Wu K, Guan Y, Tang J, Liang J, Sun R, Guo Z, Zheng S, Wu X, Jiang H, Jiang X, Zhong B, Niu X, Sun S, Wang X, Chen M, Fu G Tags: Int J Mol Med Source Type: research

Conditioned medium derived from human amniotic stem cells delays H2O2 ‑induced premature senescence in human dermal fibroblasts.
In conclusion, the findings indicated that the paracrine effects derived from human amniotic stem cells aided delaying oxidative stress‑induced premature senescence. PMID: 31545472 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - September 20, 2019 Category: Molecular Biology Authors: Pan C, Lang H, Zhang T, Wang R, Lin X, Shi P, Zhao F, Pang X Tags: Int J Mol Med Source Type: research

Identification of immunodominant IgE epitopes of the major house dust mite allergen Der f 24.
Abstract Previously, a ubiquinol‑cytochrome c reductase binding protein (UQCRB) homolog was identified in the house dust mite (HDM) species Dermatophagoides farinae (Der f) as a major allergen. In the present study, the immunodominant immunoglobulin E (IgE) epitope of the protein Der f 24 was investigated. Analysis of the homologous amino acid (aa) sequences in Der f and human UQCRB was performed. Four different recombinant Der f 24 and hybrid proteins formed by integrating Der f and human UQCRB sequences were expressed in Escherichia coli, purified using Ni‑NTA resins, and IgE‑binding activity was...
Source: International Journal of Molecular Medicine - September 18, 2019 Category: Molecular Biology Authors: Cai ZL, Zhang Z, Luo WL, Hou YB, He YS, Chen JJ, Ji K Tags: Int J Mol Med Source Type: research

Incremental load training improves renal fibrosis by regulating the TGF ‑β1/TAK1/MKK3/p38MAPK signaling pathway and inducing the activation of autophagy in aged mice.
Incremental load training improves renal fibrosis by regulating the TGF‑β1/TAK1/MKK3/p38MAPK signaling pathway and inducing the activation of autophagy in aged mice. Int J Mol Med. 2019 Sep 17;: Authors: Bao C, Yang Z, Cai Q, Li Q, Li H, Shu B Abstract Recent studies have confirmed that kidney tissue fibrosis is closely linked to the natural aging of organs. One of its major characteristics is the reduction of autophagic activity. However, to date, few studies have assessed whether incremental load training is able to improve the occurrence of renal fibrosis caused by natural aging and the unde...
Source: International Journal of Molecular Medicine - September 17, 2019 Category: Molecular Biology Authors: Bao C, Yang Z, Cai Q, Li Q, Li H, Shu B Tags: Int J Mol Med Source Type: research

Comprehensive circular RNA profiling reveals the  regulatory role of the hsa_circ_0137606/miR‑1231 pathway in bladder cancer progression.
This study explored changes in circRNA expression during BC progression by sequencing different grade BC samples and normal controls to reveal the circRNA expression profiles of different BC grades. Gene Ontology (GO) and Kyoto Encyclopedia of Gens and Genomes (KEGG) pathway analyses, and protein‑protein interaction network construction were used to predict pathways that the differentially expressed circRNAs may participate in. circRNA expression levels were detected using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and dual‑luciferase reporter assays were used to investigate the interact...
Source: International Journal of Molecular Medicine - September 17, 2019 Category: Molecular Biology Authors: Li W, Li Y, Sun Z, Zhou J, Cao Y, Ma W, Xie K, Yan X Tags: Int J Mol Med Source Type: research

SKA1 overexpression is associated with the  prognosis of esophageal squamous cell carcinoma and regulates cell proliferation and migration.
SKA1 overexpression is associated with the prognosis of esophageal squamous cell carcinoma and regulates cell proliferation and migration. Int J Mol Med. 2019 Sep 17;: Authors: Hu D, Li Z, Li X, Fu H, Zhang M Abstract Spindle and kinetochore‑associated protein 1 (SKA1), a microtubule‑binding subcomplex of the outer kinetochore, is essential for complete chromosomal separation. SKA1 has been suggested as a potential biomarker for various types of cancer. However, the exact role of SKA1 in esophageal squamous cell carcinoma (ESCC) remains unclear. The present study investigated whether SKA...
Source: International Journal of Molecular Medicine - September 17, 2019 Category: Molecular Biology Authors: Hu D, Li Z, Li X, Fu H, Zhang M Tags: Int J Mol Med Source Type: research

Quantitative proteomics reveal the alterations in the spinal  cord after myocardial ischemia‑reperfusion injury in rats.
Quantitative proteomics reveal the alterations in the spinal cord after myocardial ischemia‑reperfusion injury in rats. Int J Mol Med. 2019 Sep 17;: Authors: Li SY, Li ZX, He ZG, Wang Q, Li YJ, Yang Q, Wu DZ, Zeng HL, Xiang HB Abstract There is now substantial evidence that myocardial ischemia‑reperfusion (IR) injury affects the spinal cord and brain, and that interactions may exist between these two systems. In the present study, the spinal cord proteomes were systematically analyzed after myocardial IR injury, in an attempt to identify the proteins involved in the processes. The myocardial ...
Source: International Journal of Molecular Medicine - September 17, 2019 Category: Molecular Biology Authors: Li SY, Li ZX, He ZG, Wang Q, Li YJ, Yang Q, Wu DZ, Zeng HL, Xiang HB Tags: Int J Mol Med Source Type: research

Isosteviol prevents the development of isoprenaline ‑induced myocardial hypertrophy.
In conclusion, the present study demonstrated that STVNa protects against Iso‑induced myocardial hypertrophy by reducing oxidative stress, restoring ΔΨm and maintaining Ca2+ homeostasis. PMID: 31545484 [PubMed - as supplied by publisher] (Source: International Journal of Molecular Medicine)
Source: International Journal of Molecular Medicine - September 17, 2019 Category: Molecular Biology Authors: Chen Y, Beng H, Su H, Han F, Fan Z, Lv N, Jovanović A, Tan W Tags: Int J Mol Med Source Type: research

Kappa opioid receptor agonists improve postoperative cognitive dysfunction in rats via the JAK2/STAT3 signaling pathway.
Abstract Postoperative cognitive dysfunction (POCD) is a common and well‑known complication following surgery, particularly cardiopulmonary bypass (CPB) surgery. There are currently no suitable treatments for POCD, which is associated with increased illness and mortality rates. The present study aimed to identify a novel treatment for POCD. The protective effect of kappa opioid receptor (KOR) agonists on POCD in rats following CPB was determined and the regulatory mechanism of the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway was examined. The rats were randomly...
Source: International Journal of Molecular Medicine - September 13, 2019 Category: Molecular Biology Authors: Li X, Sun Y, Jin Q, Song D, Diao Y Tags: Int J Mol Med Source Type: research

Role of IGFBP1 in the senescence of vascular endothelial cells and severity of aging ‑related coronary atherosclerosis.
Role of IGFBP1 in the senescence of vascular endothelial cells and severity of aging‑related coronary atherosclerosis. Int J Mol Med. 2019 Sep 12;: Authors: Wu X, Zheng W, Jin P, Hu J, Zhou Q Abstract The senescence of vascular endothelial cells (ECs) plays a critical role in aging‑related cardiovascular diseases. We previously reported the causal relation of Jagged1 in ECs and the thickening of the arterial wall in aging mice. The aim of the present study was to further investigate the correlation between insulin‑like growth factor‑binding protein 1 (IGFBP1), one of the secretory proteins reg...
Source: International Journal of Molecular Medicine - September 12, 2019 Category: Molecular Biology Authors: Wu X, Zheng W, Jin P, Hu J, Zhou Q Tags: Int J Mol Med Source Type: research