A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - April 23, 2024 Category: Cytology Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
A Novel DNase Assay Reveals Low DNase Activity in Severe Asthma
Am J Physiol Lung Cell Mol Physiol. 2024 Apr 23. doi: 10.1152/ajplung.00081.2024. Online ahead of print.ABSTRACTSecreted deoxyribonucleases (DNases), such as DNase-1 and DNase-IL3, degrade extracellular DNA, and endogenous DNases have roles in resolving airway inflammation and guarding against autoimmune responses to nucleotides. Subsets of patients with asthma have high airway DNA levels, but information about DNase activity in health and in asthma is lacking. To characterize DNase activity in health and in asthma, we developed a novel kinetic assay using a Taqman probe sequence that is quickly cleaved by DNase-I to produ...
Source: Am J Physiol Lung Ce... - April 23, 2024 Category: Respiratory Medicine Authors: Annabelle R Charbit Maude A Liegeois Wilfred W Raymond Suzy A A Comhair Mats W Johansson Annette T Hastie Eugene R Bleecker Merritt Fajt Mario Castro Kaharu Sumino Serpil C Erzurum Elliot Israel Nizar N Jarjour David T Mauger Wendy C Moore Sally E Wenzel Source Type: research
5Z ‐7‐Oxozaenol attenuates cuprizone‐induced demyelination in mice through microglia polarization regulation
ConclusionThese findings suggest that inhibiting TAK1 may be an effective approach for treating demyelinating diseases. (Source: Brain and Behavior)
Source: Brain and Behavior - April 23, 2024 Category: Neurology Authors: Shiyu Chen,
Siyao Liu,
Yalun Huang,
Shiwen Huang,
Wanzhou Zhang,
Huifang Xie,
Lingli Lu Tags: ORIGINAL ARTICLE Source Type: research
GSE264288 The effect of CRISPLD2 on TGF β-induced fibroblast fibrosis
In this study, human cysteine-rich secreted protein LCCL domain protein 2 (CRISPLD2) was found increased reactivity in TGFβ induced fibroblasts, and we further confirmed that CRISPLD2 can participate in TGFβ induced fibroblast fibrosis from multiple perspectives and levels, and investigated the mechanism of CRISPLD2 in fibrosis, providing evidence for CRISPLD2 to become a clinical potentia l therapeutic target and a new biomarker. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - April 23, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
A Prospective Multimodal Education Intervention for Providers Does Not Increase Hepatic Encephalopathy Treatment Rates
ConclusionThis MMEI did not increase HE treatment rates, suggesting that alternative strategies are needed to identify and address barriers to treatment. (Source: Digestive Diseases and Sciences)
Source: Digestive Diseases and Sciences - April 23, 2024 Category: Gastroenterology Source Type: research
Retrospective Analysis of Efgartigimod Use in Patients with Double-seronegative Generalized Myasthenia Gravis: A Case Series
Myasthenia gravis (MG), an autoimmune disease caused by autoantibodies that target proteins at the neuromuscular junction (NMJ), leads to impairment in synaptic transmission at the NMJ, resulting in variable weakness and skeletal muscle fatigability in this disease [1]. MG is classified based on clinical phenotype and type of autoantibodies involved. Most patients (75% –80%) are seropositive for antibodies against acetylcholine receptor (AChR), which consist primarily of IgG1 and IgG3 subtypes; their binding reduces the number and function of AChRs. (Source: Neuromuscular Disorders)
Source: Neuromuscular Disorders - April 23, 2024 Category: Neurology Authors: Kazuhiro Horiuchi, Shuntaro Nakamura, Kazuki Yamada, Takashi Inoue, Kei Oiwa Tags: Case report Source Type: research
Emerging Therapeutics in Chronic Urticaria
Chronic urticaria (CU) is a common and long-lasting mast cell-mediated skin disease associated with psychiatric and autoimmune comorbidities, high economic costs, and considerable impact on quality of life. Available therapies show limited efficacy in many CU patients, which may be related to distinct underlying pathophysiology. Targeted and disease-modifying treatments with higher and broader efficacy are needed and are under development for CU. These novel drugs, small molecules, and monoclonal antibodies target mast cells and their receptors, signaling pathways, or mediators and other immune cells. In this article, the ...
Source: Immunology and Allergy Clinics of North America - April 23, 2024 Category: Allergy & Immunology Authors: Melba Mu ñoz, Emek Kocatürk, Marcus Maurer, Pavel Kolkhir Source Type: research
EMAS position statement: Thyroid disease and menopause
Women experience menopause at a particular stage of their lifetime. On the other hand, subclinical hypothyroidism, mainly due to autoimmune thyroiditis, is a common endocrine disease in women during their reproductive years (the prevalence rate is 6 –10 %) [1] and is often present or arises during the menopausal transition. (Source: Maturitas)
Source: Maturitas - April 23, 2024 Category: Primary Care Authors: Gesthimani Mintziori, Stavroula Veneti, Kris Poppe, Dimitrios G. Goulis, Eleni Armeni, C. Tamer Erel, Ivan Fistoni ć, Timothy Hillard, Angelica Lindén Hirschberg, Blazej Meczekalski, Nicolás Mendoza, Alfred O. Mueck, Tommaso Simoncini, Petra Stute, Dor Tags: Original article Source Type: research
Hepatic and Extrahepatic Characteristics of Autoimmune Hepatitis: A 23-year Hospital-Based Cohort Study
ConclusionIn an Asian country endemic for viral hepatitis, the female-to-male and baseline cirrhosis rates of AIH patients were lower than expected, while over 60% of the patients eventually developed cirrhosis. The high posttherapy relapse rate warrants cautious monitoring, particularly for patients with high baseline Alk-p levels. Baseline age, cirrhosis status and ANA titers are crucial for outcomes. (Source: Digestive Diseases and Sciences)
Source: Digestive Diseases and Sciences - April 23, 2024 Category: Gastroenterology Source Type: research
A Prospective Multimodal Education Intervention for Providers Does Not Increase Hepatic Encephalopathy Treatment Rates
ConclusionThis MMEI did not increase HE treatment rates, suggesting that alternative strategies are needed to identify and address barriers to treatment. (Source: Digestive Diseases and Sciences)
Source: Digestive Diseases and Sciences - April 23, 2024 Category: Gastroenterology Source Type: research
Hepatic and Extrahepatic Characteristics of Autoimmune Hepatitis: A 23-year Hospital-Based Cohort Study
ConclusionIn an Asian country endemic for viral hepatitis, the female-to-male and baseline cirrhosis rates of AIH patients were lower than expected, while over 60% of the patients eventually developed cirrhosis. The high posttherapy relapse rate warrants cautious monitoring, particularly for patients with high baseline Alk-p levels. Baseline age, cirrhosis status and ANA titers are crucial for outcomes. (Source: Digestive Diseases and Sciences)
Source: Digestive Diseases and Sciences - April 23, 2024 Category: Gastroenterology Source Type: research
