Potential therapeutic options for celiac Disease: An update on Current evidence from Gluten-Free diet to cell therapy
Int Immunopharmacol. 2024 Apr 11;133:112020. doi: 10.1016/j.intimp.2024.112020. Online ahead of print.ABSTRACTCeliac disease (CD) is a chronic autoimmune enteropathy and multifactorial disease caused by inappropriate immune responses to gluten in the small intestine. Weight loss, anemia, osteoporosis, arthritis, and hepatitis are among the extraintestinal manifestations of active CD. Currently, a strict lifelong gluten-free diet (GFD) is the only safe, effective, and available treatment. Despite the social burden, high expenses, and challenges of following a GFD, 2 to 5 percent of patients do not demonstrate clinical or pa...
Source: International Immunopharmacology - April 12, 2024 Category: Allergy & Immunology Authors: Effat Noori Nader Hashemi Delsuz Rezaee Reza Maleki Forough Shams Bahram Kazemi Mojgan Bandepour Fardin Rahimi Source Type: research
Disruption of IFN γ, GZMB, PRF1, or LYST Results in Reduced Suppressive Function in Human CD8+ T Cells
In this study, we further investigated the importance of IFNγ-, GZMB-, PRF1-, and LYST-associated pathways in CD8+ T cell-mediated suppression. Using the CRISPR-Cas9 ribonucleoprotein transfection system, we first optimized efficient gene knockout while maintaining high viability in primary bulk human CD8+ T cells. Knockout was confirmed through quantitative real-time PCR assays in all cases, combined with flow cytometry where appropriate, as well as confirmation of insertions and/or deletions at genomic target sites. We observed that the knockout of IFNγ, GZMB, PRF1, or LYST, but not the knockout of IL4 or IL5, resulted...
Source: Journal of Immunology - April 12, 2024 Category: Allergy & Immunology Authors: Chakrapani Vemulawada Pranav S Renavikar Michael P Crawford Scott Steward-Tharp Nitin J Karandikar Source Type: research
A Rare Case of Benign Recurrent Intrahepatic Cholestasis Initially Diagnosed in Middle-age
CONCLUSION: The comprehensive diagnosis of a middle-aged male patient with BRIC-2, which involved extensive radiological, hematological, and genetic investigations, informed a tailored tapering prednisone regimen, highlighting the importance of personalized medicine in managing atypical presentations of this rare cholestatic disorder.PMID:38607191 (Source: Alternative Therapies in Health and Medicine)
Source: Alternative Therapies in Health and Medicine - April 12, 2024 Category: Complementary Medicine Authors: Fang Liu Wei Li Ming-Gang Zhang Zhen-Mei Song Xiao-di Wang Xiu-Hong Wang Shi-Yu Du Source Type: research
Safety of Anti-Reelin Therapeutic Approaches for Chronic Inflammatory Diseases
In this study, we have investigated the safety and efficacy of selectively depleting plasma Reelin as a potential therapeutic strategy for chronic inflammatory diseases. We found that Reelin expression remains stable throughout adulthood and that peripheral anti-Reelin antibody treatment with CR-50 efficiently depletes plasma Reelin without affecting its levels or functionality within the CNS. Notably, this approach preserves essential neuronal functions and synaptic plasticity. Furthermore, in mice induced with experimental autoimmune encephalomyelitis (EAE), selective modulation of endothelial responses by anti-Reelin an...
Source: Atherosclerosis - April 12, 2024 Category: Cardiology Authors: Laurent Calvier Anna Alexander Austin T Marckx Maria Z Kounnas Murat Durakoglugil Joachim Herz Source Type: research
Cardiovascular risk and inflammation in a population with autoimmune diseases: a narrative review
Front Immunol. 2024 Mar 28;15:1380372. doi: 10.3389/fimmu.2024.1380372. eCollection 2024.ABSTRACTJuvenile Systemic Connective Tissue Diseases (JSCTD) are a heterogeneous group of chronic autoimmune diseases, associated with dyslipidemia and increased cardiovascular risk are related. Studies from the last 10 years, from 2013 to 2022, on lipid profiles in JSCTD were collected. Different studies on lipid profiles in children affected by JSCTD were selected, because the aim is to analyze the cardiovascular risk and the possibility of atherosclerosis in these patients in whom, sometimes, corticosteroid therapies and immunosuppr...
Source: Atherosclerosis - April 12, 2024 Category: Cardiology Authors: Camilla Bertoni Alessandra Mazzocchi Ludovica Leone Carlo Agostoni Giovanni Filocamo Source Type: research
A case of multiple autoimmune syndrome comprising autoimmune thyroid disease, vitiligo, morphea, and lichen sclerosus
We report a unique case of a 55-year-old female patient that presented with four autoimmune diseases: autoimmune thyroid disease, vitiligo, morphea, and lichen sclerosus. She was evaluated for progression of morphea and lichen sclerosus, and we confirmed histopathological overlapping of these two diseases in the same lesion. We discuss the increasing prevalence of autoimmune diseases and similar case reports on dermatological polyautoimmunity.PMID:38606648 (Source: Acta Dermatovenerologica Alpina, Panonica, et Adriatica)
Source: Acta Dermatovenerologica Alpina, Panonica, et Adriatica - April 12, 2024 Category: Dermatology Authors: Hana Ga šper Vesna Breznik Source Type: research
Collage of cases and brief review of the laboratory diagnosis and molecular testing in autoimmune haemolytic anaemia
J Family Med Prim Care. 2024 Feb;13(2):409-416. doi: 10.4103/jfmpc.jfmpc_2131_22. Epub 2024 Mar 6.ABSTRACTAutoimmune haemolytic anaemia (AIHA) is an acquired heterogenous clinical entity with variable presentations like acute haemolysis or mild, chronic haemolysis compounded with acute exacerbation in winters or fatal uncompensated haemolysis. A step-wise approach to the diagnosis and characterisation of AIHA should be undertaken, firstly the diagnosis of haemolysis followed by the establishment of immune nature with the aid of direct agglutination tests (DAT). Simultaneously the other causes of immune haemolysis need to b...
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Harkiran Arora Shalini Trivedi Pooja Jain Udita Singhal Arunpreet Kaur Aditi Raina Source Type: research
Thyroidectomy for Euthyroid Patients with Hashimoto Disease and Persistent Symptoms: An Observational, Postrandomization Study
CONCLUSIONS: Thyroidectomy had a beneficial symptom-reducing effect in euthyroid patients with Hashimoto disease and persistent symptoms. The pathophysiology of residual symptoms remains unclear, and surgical complication rates are high. If thyroidectomy is considered as a treatment option, it should be performed in dedicated centers with experienced endocrine surgeons and as part of further studies on persistent symptoms. This trial is registered with NCT-02319538.PMID:38606313 | PMC:PMC11008973 | DOI:10.1155/2024/5518720 (Source: Journal of Thyroid Research)
Source: Journal of Thyroid Research - April 12, 2024 Category: Endocrinology Authors: Geir Hoff Tomm Bernklev Lene Johnsen Laurens Reitsma Dirk Sina Andromeda Lauzike Charlotte Gibbs Tone Hoel Lende Jon Kristian Narvestad Rasmus Kildahl Roald Omdal Jan Terje Kval øy H åvard Søiland Source Type: research
Autoimmune comorbidities associated with sarcoidosis: a case-control study in the All of Us research program
CONCLUSION: We demonstrate an association between sarcoidosis and multiple autoimmune diseases in a large and diverse cohort based in the USA. These results underscore the need for careful screening of sarcoidosis patients for concomitant autoimmune disease.PMID:38606002 | PMC:PMC11007907 | DOI:10.1093/rap/rkad030 (Source: Adv Data)
Source: Adv Data - April 12, 2024 Category: Epidemiology Authors: Michael J Murphy Princess Edemobi Audrey C Leasure Mridu Gulati Edward J Miller William Damsky Jeffrey M Cohen Source Type: research
CRISPR-Cas9 applications in T cells and adoptive T cell therapies
Cell Mol Biol Lett. 2024 Apr 12;29(1):52. doi: 10.1186/s11658-024-00561-1.ABSTRACTT cell immunity is central to contemporary cancer and autoimmune therapies, encompassing immune checkpoint blockade and adoptive T cell therapies. Their diverse characteristics can be reprogrammed by different immune challenges dependent on antigen stimulation levels, metabolic conditions, and the degree of inflammation. T cell-based therapeutic strategies are gaining widespread adoption in oncology and treating inflammatory conditions. Emerging researches reveal that clustered regularly interspaced palindromic repeats-associated protein 9 (C...
Source: Mol Biol Cell - April 12, 2024 Category: Molecular Biology Authors: Xiaoying Chen Shuhan Zhong Yonghao Zhan Xuepei Zhang Source Type: research
CRISPR-Cas9 applications in T cells and adoptive T cell therapies
Cell Mol Biol Lett. 2024 Apr 12;29(1):52. doi: 10.1186/s11658-024-00561-1.ABSTRACTT cell immunity is central to contemporary cancer and autoimmune therapies, encompassing immune checkpoint blockade and adoptive T cell therapies. Their diverse characteristics can be reprogrammed by different immune challenges dependent on antigen stimulation levels, metabolic conditions, and the degree of inflammation. T cell-based therapeutic strategies are gaining widespread adoption in oncology and treating inflammatory conditions. Emerging researches reveal that clustered regularly interspaced palindromic repeats-associated protein 9 (C...
Source: Cellular and Molecular Biology Letters - April 12, 2024 Category: Biochemistry Authors: Xiaoying Chen Shuhan Zhong Yonghao Zhan Xuepei Zhang Source Type: research
Potential therapeutic options for celiac Disease: An update on Current evidence from Gluten-Free diet to cell therapy
Int Immunopharmacol. 2024 Apr 11;133:112020. doi: 10.1016/j.intimp.2024.112020. Online ahead of print.ABSTRACTCeliac disease (CD) is a chronic autoimmune enteropathy and multifactorial disease caused by inappropriate immune responses to gluten in the small intestine. Weight loss, anemia, osteoporosis, arthritis, and hepatitis are among the extraintestinal manifestations of active CD. Currently, a strict lifelong gluten-free diet (GFD) is the only safe, effective, and available treatment. Despite the social burden, high expenses, and challenges of following a GFD, 2 to 5 percent of patients do not demonstrate clinical or pa...
Source: International Immunopharmacology - April 12, 2024 Category: Allergy & Immunology Authors: Effat Noori Nader Hashemi Delsuz Rezaee Reza Maleki Forough Shams Bahram Kazemi Mojgan Bandepour Fardin Rahimi Source Type: research
Disruption of IFN γ, GZMB, PRF1, or LYST Results in Reduced Suppressive Function in Human CD8+ T Cells
In this study, we further investigated the importance of IFNγ-, GZMB-, PRF1-, and LYST-associated pathways in CD8+ T cell-mediated suppression. Using the CRISPR-Cas9 ribonucleoprotein transfection system, we first optimized efficient gene knockout while maintaining high viability in primary bulk human CD8+ T cells. Knockout was confirmed through quantitative real-time PCR assays in all cases, combined with flow cytometry where appropriate, as well as confirmation of insertions and/or deletions at genomic target sites. We observed that the knockout of IFNγ, GZMB, PRF1, or LYST, but not the knockout of IL4 or IL5, resulted...
Source: Journal of Immunology - April 12, 2024 Category: Allergy & Immunology Authors: Chakrapani Vemulawada Pranav S Renavikar Michael P Crawford Scott Steward-Tharp Nitin J Karandikar Source Type: research
Collage of cases and brief review of the laboratory diagnosis and molecular testing in autoimmune haemolytic anaemia
J Family Med Prim Care. 2024 Feb;13(2):409-416. doi: 10.4103/jfmpc.jfmpc_2131_22. Epub 2024 Mar 6.ABSTRACTAutoimmune haemolytic anaemia (AIHA) is an acquired heterogenous clinical entity with variable presentations like acute haemolysis or mild, chronic haemolysis compounded with acute exacerbation in winters or fatal uncompensated haemolysis. A step-wise approach to the diagnosis and characterisation of AIHA should be undertaken, firstly the diagnosis of haemolysis followed by the establishment of immune nature with the aid of direct agglutination tests (DAT). Simultaneously the other causes of immune haemolysis need to b...
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Harkiran Arora Shalini Trivedi Pooja Jain Udita Singhal Arunpreet Kaur Aditi Raina Source Type: research
Thyroidectomy for Euthyroid Patients with Hashimoto Disease and Persistent Symptoms: An Observational, Postrandomization Study
CONCLUSIONS: Thyroidectomy had a beneficial symptom-reducing effect in euthyroid patients with Hashimoto disease and persistent symptoms. The pathophysiology of residual symptoms remains unclear, and surgical complication rates are high. If thyroidectomy is considered as a treatment option, it should be performed in dedicated centers with experienced endocrine surgeons and as part of further studies on persistent symptoms. This trial is registered with NCT-02319538.PMID:38606313 | PMC:PMC11008973 | DOI:10.1155/2024/5518720 (Source: Journal of Thyroid Research)
Source: Journal of Thyroid Research - April 12, 2024 Category: Endocrinology Authors: Geir Hoff Tomm Bernklev Lene Johnsen Laurens Reitsma Dirk Sina Andromeda Lauzike Charlotte Gibbs Tone Hoel Lende Jon Kristian Narvestad Rasmus Kildahl Roald Omdal Jan Terje Kval øy H åvard Søiland Source Type: research
